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PLS3 Rabbit pAb

Catalog No.: A3627

Basic Information Background

Catalog No. Plastins are a family of actin-binding proteins that are conserved throughout eukaryote A3627 evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Observed MW Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in 71kDa the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have Calculated MW replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). 65kDa/69kDa/70kDa The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing Category results in multiple transcript variants. Primary antibody

Applications WB, IHC, IP

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:200 - 1:2000 Gene ID Swiss Prot 5358 P13797 IHC 1:50 - 1:100 Immunogen 1:50 - 1:100 IP Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2).

Synonyms PLS3;BMND18;T-plastin;plastin-3

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using PLS3 antibody (A3627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PLS3 antibody (A3627). Western blot was performed from the immunoprecipitate using PLS3 antibody (A3627) at a dilition of 1:1000.

Immunohistochemistry of paraffin- embedded human stomach using PLS3 antibody (A3627) at dilution of 1:100 (40x lens).

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