sign in sign up
<<
Home , 40S ribosomal protein S9, Activating protein 2, ADPRHL1, AKAP10, ALG14, Annexin A13

Temporal Induction Pattern of STAT4 Target Defines Potential for Th1 Lineage-Specific Programming

This information is current as Seth R. Good, Vivian T. Thieu, Anubhav N. Mathur, Qing of October 1, 2021. Yu, Gretta L. Stritesky, Norman Yeh, John T. O'Malley, Narayanan B. Perumal and Mark H. Kaplan J Immunol published online 26 August 2009 http://www.jimmunol.org/content/early/2009/08/26/jimmuno l.0901411 Downloaded from

Supplementary http://www.jimmunol.org/content/suppl/2009/08/26/jimmunol.090141 Material 1.DC1 http://www.jimmunol.org/

Why The JI? Submit online.

• Rapid Reviews! 30 days* from submission to initial decision

• No Triage! Every submission reviewed by practicing scientists

• Fast Publication! 4 weeks from acceptance to publication by guest on October 1, 2021 *average

Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts

The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2009 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published August 26, 2009, doi:10.4049/jimmunol.0901411 The Journal of Immunology

Temporal Induction Pattern of STAT4 Target Genes Defines Potential for Th1 Lineage-Specific Programming1

Seth R. Good,2* Vivian T. Thieu,2† Anubhav N. Mathur,† Qing Yu,† Gretta L. Stritesky,† Norman Yeh,† John T. O’Malley,† Narayanan B. Perumal,3* and Mark H. Kaplan3†

STAT4 is a critical component in the development of inflammatory adaptive immune responses. It has been extensively charac- terized as a lineage-determining factor in Th1 development. However, the genetic program activated by STAT4 that results in an inflammatory type is not well defined. In this report, we use DNA isolated from STAT4- immunoprecipitation to perform chromatin immunoprecipitation-on-chip analysis of over 28,000 mouse promoters to identify STAT4 targets. We demonstrate that STAT4 binds multiple gene-sets that program distinct components of the Th1 lineage. Although many STAT4 target genes display STAT4-dependent IL-12-inducible expression, other genes displayed IL-12-induced modifications but lack induction, possibly due to high relative basal expression. In the subset of genes that STAT4 programs for expression in Th1 Downloaded from cells, IL-12-induced mRNA levels remain increased for a longer time than mRNA from genes that are not programmed. This suggests that STAT4 binding to target genes, while critical, is not the only determinant for STAT4-dependent gene programming during Th1 differentiation. The Journal of Immunology, 2009, 183: 3839–3847.

he Th1 cell is responsible for cell-mediated immune tin immunoprecipitation(ChIP)4-on-chip) or large-scale se- functions including eradication of intracellular patho- quencing of the bound targets (ChIP-seq) to identify, charac- gens. STAT4 is required for the development of Th1 terize, and analyze of genes controlled by http://www.jimmunol.org/ T ϩ cells from naive CD4 T cells and most IL-12-stimulated func- transcription factors (13, 14). These studies provide a global tions (1, 2). STAT4-deficient mice are highly susceptible to picture of -target gene interactions in the infection by intracellular pathogens but are resistant to - physiological state. Such a ChIP-on-chip study may reveal mediated autoimmune diseases (1). In , STAT4 also me- STAT4-mediated transcriptional regulatory networks active in diates IL-12 functions and single polymorphisms in Th1 cell development. the STAT4 gene correlate with susceptibility to autoimmune In this report, we have performed ChIP-on-chip experiments for disease (3, 4). How STAT4 establishes the Th1 genetic program STAT4. Using informatics and biological interrogation of the data,

remains unclear. we have identified additional aspects of the STAT4-dependent ge- by guest on October 1, 2021 Following activation, STAT4 binds to cis-regulatory regions netic program and activation patterns that predict potential pro- of several genes to induce , though relatively gramming of Th1-specific gene expression. Moreover, we identify few of these have been directly identified. In humans STAT4 additional STAT4 target genes that may play a role in the inflam- has been shown to bind to IFNG and IL12RB2, and in mouse matory cell phenotype. These data establish a framework for ge- Ifng, Hlx1, and Il18r1 (5–10). However, a much larger set of nome-wide understanding of the transcription factor network that genes appears to rely on STAT4 for expression in the Th1 dif- regulates inflammatory immune responses. ferentiation program (9, 11, 12). It is possible that the ability of STAT4 to activate an inflammatory genetic program can be Materials and Methods understood better by a genome-wide analysis of target gene T cell preparation and analysis promoters. Recently, a number of studies have used chromatin For ChIP-on-chip experiments CD4ϩ T cells from C57BL/6 mice were acti- immunoprecipitation followed by microarray analysis (chroma- vated for 3 days with anti-CD3 and anti-CD28. Cells were washed and incu- bated in the presence or absence of 5 ng/ml IL-12 for 4 h before being pro- cessed for ChIP analysis by Genpathway. For analysis of gene induction and histone modification, wild-type and Stat4Ϫ/Ϫ BALB/c mice were activated for *School of Informatics, Indiana University-Purdue University Indianapolis; and 3 days as above before RNA was isolated from cells that were unstimulated or †Departments of Pediatrics, H.B. Wells Center for Pediatric Research and De- stimulated with 5 ng/ml IL-12 for 4 or 18 h. Generation of Th1 cultures and partment of Microbiology and Immunology, Indiana University School of Med- quantitative (real-time) PCR (qPCR) was performed as described (15). ELISA icine, Indianapolis, IN 46202 for TNF-␣ was performed using reagents from BD Pharmingen. DNA affinity Received for publication May 5, 2009. Accepted for publication July 16, 2009. precipitation assay and ChIP was performed as described (8). ERK immuno- blots were performed using standard methods (8). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance FactorPath ChIP-on-chip with 18 U.S.C. Section 1734 solely to indicate this fact. 1 This work was supported by Public Health Service Grant AI45515 (to M.H.K.). G.L.S., Preparation of cells for ChIP-on-chip was performed at GenPathway (16). N.Y., and J.T.O. were supported by T32AI060519; V.T.T. was supported by T32HL007910. Cells prepared as above were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine. Cell lysates were sonicated to an average 2 S.R.G. and V.T.T. contributed equally to this study. 3 Address correspondence and reprint requests to Dr. Mark H. Kaplan, Depart- ments of Pediatrics, and Microbiology, and Immunology, Indiana University 4 Abbreviations used in this paper: ChIP, chromatin immunoprecipitation; qPCR, School of Medicine, H.B. Wells Center for Pediatric Research, 702 Barnhill quantitative (real-time) PCR; GO, ; TSS, transcription start site; Drive, RI 2600, Indianapolis, IN 46202 or Dr. Narayanan B. Perumal, School of MEME, multiple expectation maximization for motif elicitation. Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202. E-mail address: [email protected] or [email protected] Copyright © 2009 by The American Association of Immunologists, Inc. 0022-1767/09/$2.00

www.jimmunol.org/cgi/doi/10.4049/jimmunol.0901411 3840 STAT4 TARGET GENES IN Th1 CELLS Downloaded from

FIGURE 1. STAT4 binding in target gene promoters. A, Wild-type CD4ϩ T cells were activated for 3 days and cultured in the presence or absence of http://www.jimmunol.org/ IL-12 for 4 h. ChIP analysis was performed for STAT4 or control IgG and qPCR was performed to determine STAT4 binding at the indicated target sequences. The scale indicates relative binding that is normalized to input DNA. B, The number of genes was graphed against binding intensity. Intensity of 4669 intervals ranged from 2.2–32.4. The cut-off intensity for subsequent analysis was 4. C, Histograms of the frequency of STAT4 binding across the arrayed sequences relative to the TSS. The curated list has all 1540 genes with binding intensity Ͼ4. The exact match list is a subset of 660 genes that contain a consensus STAT4 in the promoter. D, Comparison of the frequency of subsets of genes in the curated list (binding intensity Ͼ4) and the frequency on the Affymetrix chip using GOstat to assign gene function. p values for significant differences are indicated. E, Venn diagram comparing the overlap of IL-12-induced genes from published microarray analysis (see text) with the ChIP-on-chip dataset. by guest on October 1, 2021 DNA fragment length of 300–500 bp. Genomic DNA from aliquots was under Th1 conditions for 0, 2, 6, and 48 h (MG-U74Av2 arrays containing purified for use as input. Chromatin was precleared with A agarose 12,488 probes) (12), differentiated C57BL/6 mouse Th1 cells stimulated beads (Invitrogen) and STAT4-bound DNA sequences were isolated using with IL-12 for 18 h (custom array with 24,000 probes) (11), differentiated specific Abs (Santa Cruz sc-486). Complexes were precipitated with pro- Th1 cells stimulated with IL-12 for 4 or 24 h (HuGeneFL array tein A agarose beads, washed, eluted from the beads with SDS buffer, and containing 6000 genes) (17), and PHA/IL-2 activated human T cells stim- subjected to RNase and proteinase K treatment. Crosslinks were reversed ulated with IL-12 for 6 h (U133A and U133B arrays) (18). by incubation overnight at 65°C, and ChIP DNA was purified by phenol- chloroform extraction and ethanol precipitation. qPCR reactions were con- Multiple expectation maximization for motif elicitation (MEME) ducted in triplicate on specific genomic regions using SYBR Green Su- analysis permix (Bio-Rad). The resulting signals were normalized for primer Ͼ efficiency by carrying out quantitative PCR for each primer pair using The 1111 interval sequences with peak intensity 4, which were in close input DNA. proximity to 1540 genes, were screened by MEME (19) to find over-rep- Analysis of ChIP DNA by Affymetrix tiling arrays was conducted by resented motifs. The parameters for MEME included looking at the reverse GenPathway. ChIP (multiple reactions) and input were amplified complement sequence, a maximum motif width of 15, and a cutoff of 30 using ligation-mediated PCR. The resulting amplified DNA was purified, motifs ordered by increasing E-value. The analysis was run using three quantified, and tested by qPCR at the same specific genomic regions as the different approaches regarding the number of times the motif repeated, any original ChIP DNA to assess quality of the amplification reactions. Am- number of repeats, zero or one repeat, and one or more repeats with vir- plified DNA was fragmented and labeled using the DNA Terminal Label- tually the same results each time. WebLogo (20) generated the motif logos ing Kit from Affymetrix, and hybridized to the Affymetrix GeneChip using the aligned site occurrences for each motif given by MEME. Mouse Promoter 1.0R Array at 45°C overnight. This array contains over Gene ontology (GO) analysis 4.6 million 25-mer oligos tiled to interrogate over 28,000 mouse promoter regions (Ϫ7.5 kb to ϩ2.5kb relative to the transcription start site). Arrays The GO analysis was performed by GOstat (21). Using an input list and a were scanned and the resulting CEL files were analyzed using Affymetrix selected background, GOstat determines enriched GO terms based on a TAS software that computed estimates of fold enrichment over hybrid- Fisher exact test. The p-value from the Fisher exact test is altered using a ization with input DNA in linear scale. This metric is referred to as peak Benjamini correction. Only terms with a corrected p-value less than 0.1 are binding intensity. Thresholds were selected, and the resulting BED files reported. The analysis was performed three times with the curated gene list containing lists of sequence intervals with significant binding detected as the input and the Affymetrix chip gene list as the background. were analyzed using Genpathway software that provides comprehensive information on genomic annotation, peak metrics, and sample compar- Results isons for all peaks (intervals). Graphing of peak binding values were generated using the Affymetrix Integrated Genome Browser. The entire dataset Generation and analysis of STAT4 ChIP-on-chip data is submitted to the NCBI GEO database (GSE16845; www.ncbi.nlm. To identify STAT4 target genes, CD4ϩ T cells were activated for nih.gov/geo/query/acc.cgi?accϭGSE16845). Intersects of our dataset with previously published datasets were deter- three days with anti-CD3 before washed cells were stimulated with mined using Microsoft Excel. Published datasets that were used for anal- IL-12 for 4 h and chromatin was immunoprecipitated with anti- ysis of common genes were from naive BALB/c mouse Th cells cultured STAT4 Ab. The preactivation was performed to allow cells to The Journal of Immunology 3841 acquire IL-12 responsiveness (22). IL-12 stimulation of activated cells resulted in 70–80% of cells in the population becoming pSTAT4-positive with a 6-fold increase in mean fluorescence in- tensity, as assessed by intracellular staining (data not shown). To confirm STAT4 binding to target genes, we tested Il18r1 promoter and the Il2ra PRRIII element DNA in the precipitate following IL-12 stimulation (8, 23) and observed a greater than 30-fold in- duction of STAT4 binding, compared with unstimulated cells (Fig. 1A). DNA from the ChIP was then hybridized to an Affymetrix array containing sequences spanning Ϫ7.5kB to ϩ2.5kb of over 28,000 promoters in the mouse genome and enrichment was cal- culated based on comparison to an array hybridized with input DNA. In replicate experiments, the ChIP-on-chip analysis identi- fied a total of 4,669 genes as near a STAT4 binding site in vivo. The peak binding intensities (see definition in Materials and Meth- ods) of immunoprecipitated DNA hybridizing to the promoter oli- gonucleotides showed a wide range (2.2 to 32.4, with the highest value being the most intense binding) indicating the diverse of STAT4 binding to target genes. Mean and median of this data Downloaded from set were at 4.4 and 3.3, respectively (Fig. 1B). A manual inspection of the data showed many of the known STAT4 target and Th1- associated genes to have peak intensities of four or higher. Thus, we filtered the list of genes using a cutoff peak intensity of 4 (1111 binding sites corresponding to 1540 genes) referred to as the

“curated” list and all subsequent analyses were performed on this http://www.jimmunol.org/ curated gene set (Table S1).5 We confirmed binding of STAT4 to genes with a range of peak intensities from 4.5 to 21 using ChIP assay in wild-type and Stat4Ϫ/Ϫ activated T cells. The enrichment of percent input between wild-type and Stat4Ϫ/Ϫ samples was sim- ilar to the enrichment observed between IL-12 stimulated and un- stimulated cells (Figs. 1A and 2). These results further confirm the identification of STAT4 targets in this dataset.

We then analyzed the frequency of STAT4 binding between 7.5 by guest on October 1, 2021 kb upstream to 2.5 kb downstream of the transcription start site (TSS) to define chromatin location preferences for STAT4. A pre- ponderance of STAT4 binding is in and around the target gene TSS (Fig. 1C). There was no difference in frequency between the curated gene list and a subset of genes that had an exact STAT4 consensus motif (TTCNNNGAA in 660 of 1111 binding sites or 59.4%) (Fig. 3A) in the association of STAT4 with specific regions in the promoter. To determine whether STAT4 target genes were enriched for any specific category, we defined the enrichment of GO terms in the curated set compared with an Affymetrix mouse genome back- ground using the GOstat program (21). The major statistically sig- Ϫ/Ϫ nificant enrichments ( p Ͻ 0.05) were in genes involved in cyto- FIGURE 2. STAT4 binding to target genes. WT and Stat4 activated kine binding, including factors with IL activity and TNF T cells were stimulated with IL-12 for 4 h before nuclei were isolated for chromatin immunoprecipitation with anti-STAT4. Results are shown as D receptor binding activity (Fig. 1 ). There was also a significant percent input as calculated using qPCR results of amplification with prim- enrichment for nucleic acid binding factors. These analyses dem- ers specific for the promoter region of each of the genes indicated. The onstrate selectivity in STAT4 binding target genes associated with peak intensity of STAT4 binding derived from the ChIP-on-chip dataset specific cellular functions. and the fold-enrichment of STAT4 binding in WT, compared with Ϫ Ϫ Ϫ Ϫ A number of published reports have examined IL-12-induced Stat4 / cells, are indicated. STAT4 binding in the Stat4 / cells was gene expression using microarrays from human or mouse cells within 1 SD of the background (IgG control). treated with IL-12 for various times during or after Th1 differen- tiation (11, 12, 17, 18). We generated a cumulative list of IL-12- induced genes from these studies and compared them to the ChIP- Computational identification of the STAT4 consensus motif on-chip dataset. The Venn diagram in Fig. 1E identifies an overlap To define the proportion of STAT4-bound genes that contained of 72 genes (Table S2A) between these two analyses. a STAT4 consensus motif, TTCNNNGAA (24), we analyzed the curated gene set by directly examining consensus sites using a motif search algorithm. A Perl script to identify exact matches allowing the ‘NNN’ degeneracy at positions 4–6 identified mo- 5 The online version of this article contains supplemental material. tif 1 shown in Fig. 3A at a frequency of 59.4% in the curated 3842 STAT4 TARGET GENES IN Th1 CELLS Downloaded from http://www.jimmunol.org/

FIGURE 3. Motif analysis of STAT4 target genes. A, WebLogo representations of STAT4 consensus sequences identified by direct search for the TTCNNNGAA consensus (motif 1) or sequences identified by MEME motif search (motifs 2–5). The percentage of genes/intervals that contain sequences related to each motif is indicated with E-values for MEME motifs. B, Percentage of sequences in each range of peak values that contain a consensus STAT binding site (A, motif 1). Note that as percentages of sequences with the consensus decreases, the total number of sequences in a range increases (compare with Fig. 1B). C, Histogram of the proximity of motifs 4 and 5 to the consensus STAT motif 2 graphed as the frequency of each motif occurring in 100 bp intervals from the STAT consensus. D, DNA affinity purification analysis (DAPA) of motif 3 using biotinylated oligonucleotides. Total cell extracts isolated from purified CD4 T cells activated for 3 days and left unstimulated or stimulated for 4 h with IL-12 or IL-18 as indicated were incubated with

oligonucleotides before complexes were precipitated with streptavidin agarose. Precipitated were visualized using immunoblot. E, DAPA analysis by guest on October 1, 2021 as in C with the addition of competition (fold molar excess indicated) of binding with a STAT consensus (motif 3) or a nonspecific competitor. gene list. In the second approach, we used the expectation- motif 5 occurrences within 100 bp intervals relative to the po- maximization motif search algorithm, MEME (19) to identify sition of the consensus STAT site (motif 2). Motifs 4 and 5 were motifs. We observed a number of motifs with low E-values. enriched in proximity to the STAT site, almost entirely within Sequences containing variants of a STAT4 consensus site were 1 kb of the consensus sequence (Fig. 3C). We functionally as- found in all STAT4 bound sequences (Fig. 3A, motif 2), further sessed binding to motif 3 using biotinylated oligonucleotides validating the dataset and suggesting that the false-positive rate that spanned the motif. We detected STAT4 binding specifically is low. The percentage of the genes containing the consensus to motif 3 that was competed by STAT consensus oligonucle- (motif 1) was highest in sequences that had high peak values, otides to a greater extent than by nonspecific sequences (Fig. 3, and decreased as peak values decreased (Fig. 3B). Although D and E). these data shows that some sequences containing the STAT consensus site were not included in the curated dataset (peak STAT4 binds multiple genes associated with the Th1 phenotype value less than 4), the problem of the exclusion of these false- Although the Th1 genetic program is known to be largely de- negatives is offset by avoiding the inclusion of large numbers of pendent on STAT4, and STAT4 binding to a subset of Th1 genes (Fig. 1B) that would lack other identifiable motifs. If all genes has been demonstrated, all targets of STAT4 required to bound genes in the array are considered, the percentage with the establish Th1 differentiation are not clear. We examined the consensus falls to 43%, and the MEME algorithm identified curated list for genes demonstrated in the literature to be func- Ͻ10% of the genes as having motif 2 if the cut-off was reduced tionally associated with the Th1 phenotype, or preferentially to a peak value of 3. Thus, our analysis minimizes false-posi- expressed in Th1 cells, and have demonstrated STAT4- or T- tives at the expense of potentially excluding a limited number bet-dependence in their expression (Table S3). We identified of valid targets. binding to a set of genes (18/28 Th1 genes searched (Fig. 4A, Three additional motifs shown in Fig. 3A were obtained from Table S3); significantly enriched from the genome p Ͻ 10Ϫ8) the MEME analysis that were present in 5–10% of the total including Furin, Ifng, and Il18r1, genes that are known to be sequences and had low E-values. Manual and JASPAR-medi- STAT4 targets (5, 6, 8, 26) as well as Th1 genes that are ex- ated (25) sequence analysis identified a STAT-like site in motif pressed independently of STAT4 including Egr2 and Ms4a4b. Using 3, an NF-␬B site in motif 4 and a PPAR␥/RXR site in motif 5. Affymetrix’s Integrated Genome Browser we mapped the peak inten- These motifs were not significantly enriched in any GO cate- sities of binding in a subset of these genes (Fig. 4B). Although the gorized gene set. We mapped the frequency of motif 4 and microarray in these experiments is focused on promoter sequences, The Journal of Immunology 3843

FIGURE 4. STAT4 binding in Th1 genes. A, Heatmap of Th1 associated genes based on binding intensity of STAT4 to gene promoters. B, The In- tegrated Genome Browser (IGB) used BAR files generated from the ChIP-on- chip data to identify binding peaks Downloaded from relative to annotated genes. The graphs indicate the significance (p-value prob- ability) of enrichment at promoter-ar- rayed sites. C, Wild type and Stat4Ϫ/Ϫ CD4ϩ T cells were cultured under Th1 conditions for five days before RNA was isolated for qPCR using the indi- http://www.jimmunol.org/ cated primers. RNA levels are relative to wild type Th1 cells. by guest on October 1, 2021

STAT4 peak binding was largely localized to the gene regions im- served a set of genes that are associated with TCR signaling iden- mediately upstream of transcription initiation. The exceptions to this tified from the literature (29) (7/23 TCR signaling genes searched included Gadd45g, which had peaks both 5-prime and 3-prime of the (Table S2B); significant enrichment from the genome p ϭ 1 ϫ gene, and Tnf that had intragenic peak binding, closely linked to 10Ϫ4) including Cd3z, Prkcq/PKC␪, and Lcp2/SLP-76 (Fig. 5A) STAT4 binding at the Lta promoter (Fig. 4B). that are important for the stimulation of T cells in response to Several of these genes, while associated with Th1 function, specific Ag. To test whether expression of these genes was affected had not been demonstrated to be STAT4-dependent. To test a by STAT4-deficiency, we differentiated wild-type and Stat4Ϫ/Ϫ requirement for STAT4 in Th1 expression of these genes, we Th1 cells and used qPCR to examine relative levels of gene ex- Ϫ/Ϫ ϩ differentiated wild-type and Stat4 CD4 T cells under Th1 pression, using Hlx1 as a STAT4-dependent control. Although conditions and used quantitative PCR to examine relative ex- expression of Prkcq and a number of other TCR signaling genes pression. Gadd45g, which plays a role in IFN-␥ production and Ϫ Ϫ was not significantly decreased in Stat4 / Th1 cells, Lcp2 ex- potentially synergy between IL-12 and IL-18 (27), was de- Ϫ/Ϫ Ϫ Ϫ pression in Stat4 cultures was decreased to one-half the creased more than 5-fold in Stat4 / Th1 cultures compared level seen in wild type cultures (Fig. 5B). To determine whether with wild-type cultures (Fig. 4C). Myd88, which we have pre- there are consequences of this decrease in expression, we ana- viously shown to be IL-12-inducible in a STAT4-dependent manner (28), also required STAT4 for maximal expression in lyzed the TCR-induced of the ERK MAPKs Ϫ/Ϫ Th1 cells (Fig. 4C). and observed decreased ERK phosphorylation in Stat4 Th1 cells, compared with wild-type cells (Fig. 5C). These observa- STAT4 binds a subset of genes associated with TCR signaling tions agree with previous data suggesting that strong stimuli Ϫ Ϫ In a manual examination of the curated gene list, we defined sub- minimize the defect in IFN-␥ production by Stat4 / Th1 cells sets of genes that would have specific functional consequences for (30), though it is difficult to test using IFN-␥ as a readout be- T cell differentiation or function. Among these clusters we ob- cause STAT4 plays an important role in programming Ifng for 3844 STAT4 TARGET GENES IN Th1 CELLS

manually based on involvement in Th differentiation, immune re- sponses or gene expression, in wild-type and Stat4Ϫ/Ϫ activated T cells stimulated for four or 18 h with IL-12. In Fig. 6A, an analysis of genes that demonstrated STAT4-dependent IL-12 induction in- dicated that there is no correlation between binding intensity and level of gene induction. This is exemplified by Il18rap, which was one of the highest intensity STAT4 binding genes, but was induced only 2-fold at 4 h, while Il18r1, which bound with less intensity, was induced 5-fold. The three genes that showed over 20-fold induction, Furin, Ifng, and Il24, had intensity ranging from 7.5 to 21. We also performed this analysis using an IL-12-induced mi- croarray dataset (18), and observed a similar pattern when the pub- lished fold-induction was graphed against our binding intensity values (Fig. 6B). To further characterize this subset of genes, we divided genes into categories based on the patterns of gene induction by IL-12. “Noninduced” genes had Յ2-fold induction at four or 18 h; “Tran- sient” genes had Ͼ2-fold induction at4hbutϽ2-fold induction at 18 h, and “sustained” genes had Ͼ2-fold induction at 4 and 18 h. For each subset we graphed the fold IL-12-induced gene expres- Downloaded from sion vs the basal gene expression normalized to the endogenous ␤ control 2-microglobulin. We observed a general trend that genes with lower basal expression had greater induction, and observed that those STAT4 target genes not induced by IL-12 were among the most highly expressed genes that we analyzed (Fig. 6C). This suggested that the noninduced genes did not lack induction be- http://www.jimmunol.org/ cause they were repressed in T cells, but rather that expression was high and induction by IL-12 might have a more limited effect. To determine whether IL-12 was having effects on the noninduced genes we compared histone modifications at the promoters of three sustained genes and four noninduced genes. We observed that IL-12 stimulation increased H3 and decreased H3K27 methylation at both sets of genes, but had significantly greater FIGURE 5. STAT4 regulates TCR signaling. A, Heatmap of TCR sig- effects at the promoters of the induced genes (Fig. 6D). These data, by guest on October 1, 2021 naling-associated genes based on binding intensity of STAT4 to gene pro- coupled with the demonstration that STAT4 binds to the promoter Ϫ/Ϫ ϩ moters. B, Wild-type and Stat4 CD4 T cells were cultured under Th1 of Irf1 (Fig. 2), one of the noninduced genes, suggests that they are conditions for 5 days before RNA was isolated for qPCR using the indi- bona fide STAT4 targets, but that the effects of IL-12 are limited cated primers. RNA levels are relative to wild-type Th1 cells. C, Wild-type Ϫ Ϫ ϩ at these promoters for reasons that may include high basal expres- and Stat4 / CD4 T cells were cultured under Th1 conditions for 5 days before cells were stimulated with anti-CD3 for the indicated times. Total sion or lack of appropriate promoter context for induction. protein extracts were immunoblotted for phospho- and total ERK. D, Wild- Upon examining the genes that were in each group, we observed type and Stat4Ϫ/Ϫ CD4ϩ T cells were cultured under Th1 conditions for 5 some interesting patterns (Fig. 6E). Within the noninduced set days before cells were stimulated with anti-CD3 or PMA plus ionomycin were Foxp3 and Gzmb, genes not expressed in Th1 cells, and for 24 h. TNF-␣ was tested in cell-free supernatants using ELISA. Ms4a4b, a STAT4-independent Th1 gene. The transiently induced set included receptors such as Il2ra and Cd40l, and transcription factors such as Irf4, Irf8, and Bcl6 where transient expression Th1 expression (31). To avoid this problem, we analyzed might confer temporary function to the cell. The sustained subset Ϫ Ϫ TNF-␣ production from wild-type and Stat4 / Th1 cultures contained many of the genes that require STAT4 to program high stimulated with either anti-CD3 or PMA and ionomycin. Al- expression in Th1 cells including Ifng, Furin, Il18r1, and though TNF-␣ production following anti-CD3 stimulation is Gadd45g. We analyzed two additional genes that STAT4 is known Ϫ Ϫ decreased in Stat4 / cultures compared with wild-type, stim- to program but were not found on the ChIP-on-chip list, Etv5 and ulation with PMA and ionomycin, which bypasses the require- Hlx1, and found that they also were in this category. Thus, the ment for proximal signaling molecules such as SLP-76, resulted induction kinetics of STAT4 target genes following IL-12 stimu- in similar levels of TNF-␣ production (Fig. 5D). Thus, STAT4 lation of activated T cells is a strong predictor of the requirement targets genes associated with TCR signaling and contributes to for STAT4 in programming expression for Th1 cells. the potency of Ag receptor stimulation. Discussion Distinct patterns of STAT4-dependent gene induction among STAT4 is a critical regulator of adaptive inflammatory responses, STAT4 target genes yet there is still little known as to how it performs these functions. From the dataset of genes bound by STAT4, we wanted to deter- In previous work, we have shown that STAT4 binds to specific mine whether there was a relationship between the intensity of target genes and mediates programming for high levels of expres- STAT4 binding to a gene and the level of gene induction partic- sion in Th1 cells. It is still not well understood what genes are ularly because the overlap between our ChIP-on-chip targets and STAT4 targets on a genome-wide scale and how selectivity in the public domain expressed gene lists was limited (Fig. 1E). To activation is achieved. Previous microarray analyses have not pro- test this, we analyzed mRNA levels of over 30 genes, selected vided significant insight into gene programs activated by STAT4. The Journal of Immunology 3845 Downloaded from http://www.jimmunol.org/

Ϫ/Ϫ ϩ FIGURE 6. Patterns of STAT4-dependent gene induction. A, Wild-type and Stat4 CD4 T cells were activated for 3 days and stimulated with IL-12 by guest on October 1, 2021 for 4 or 18 h before RNA was isolated for qPCR. Graphs are plot of STAT4 binding intensity vs fold IL-12-stimulated gene induction at 4 h for genes that showed STAT4-dependent gene induction. B, Graph of STAT4 binding intensity vs fold-IL-12 induction of the genes identified as overlap between the ␤ ChIP-on-chip data and published microarray data (18) using fold-induction values from the microarray data. C, Graph of gene expression relative to 2m vs fold induction as determined in A. Genes that showed no induction or STAT4-dependent induction were divided into subsets based on the kinetics of expression (minimal induction, Յ 2-fold induction at 4 or 18 h; transient, Ͼ2-fold induction at 4 h, Յ 2-fold induction at 18 h; sustained induction, Ͼ2-fold induction at 4 and 18 h). D, ChIP assay for acetylated H3K9/18 or trimethylated H3K27 in purified CD4 T cells activated for three days and cultured with or without IL-12 for 4 h. qPCR for the promoters of induced genes (Furin, Ifng, IL18r1) and noninduced genes (Foxp3, Irf1, Mbd2, Ms4a4b) is expressed significantly different from induced genes (p Ͻ ,ء .as the fold-increase in acetylation or the percent decrease in H3K27 methylation from unstimulated cells 0.05) as determined by Student’s t test. E, Expression pattern of genes examined in A at 4 and 18 h after stimulation with IL-12 divided based on criteria in C. A partial list of gene names in each subset is indicated to the right of the graph.

In this report, we have used a ChIP-on-chip approach to identify 1B) would greatly skew subsequent analyses. For example, while STAT4 target genes and characterized STAT4 activity at these MEME identified a STAT-related site in 100% of the genes in our targets. curated list with a cut-off above 4, the algorithm identified Ͻ10% of One of the important issues in examining a large dataset such as genes with a similar motif when a list with a cut-off of three was used, the one in this report is the level of false positives and false neg- resulting from a large number of genes that lacked the site and de- atives identified. As we demonstrated in Fig. 3A, sequences from creased the enrichment potential for that motif. Regardless, this is a 60% of the genes in our curated list contained a consensus STAT limitation in the interpretation of this and related datasets. binding site, and 100% were identified with a match to a motif The Th1 genetic program involves many genes, which are either similar to a STAT consensus. This suggests a low rate of false- STAT4-dependent or STAT4-independent (9, 11). The 28 genes positives in the list, which is further confirmed by showing that we considered functionally linked to the Th1 phenotype are shown STAT4 binds to targets across a broad range of peak intensity in Table S3 and the STAT4 target genes in this dataset include values (Fig. 2). However, using the cut-off of peak value 4 for our (Ifng, Tnf, Lta), receptors (Il18r1, Il18rap, Il12rb2), and curated list might omit some valid STAT4 targets. When divided signaling factors (Gadd45b, Gadd45g, Myd88) (Fig. 7). STAT4 into ranges of peak values, the percent of sequences that contain a also bound Furin, which has critical roles in maintaining tolerance, STAT consensus decreases with decreasing peak value (Fig. 3B). In a function that suggests STAT4 might have both pro- and anti- the peak value 4.0–4.9 set the percentage is 50%, but decreases fur- inflammatory activities (32). It is interesting that 6 of the 20 known ther to 40% from 3.0–3.9 and 32% from 2.2–2.9, with an average of STAT4-dependent Th1 genes (Table S3), including Hlx1 and Etv5, 43% for the entire list. Thus, there might be STAT4 targets below our were not in our dataset. This likely represents either indirect acti- cut-off. However, the large number of genes in the lower ranges (Fig. vation of genes by STAT4, or STAT4 binding to target genes 3846 STAT4 TARGET GENES IN Th1 CELLS

might be compromised in Stat4Ϫ/Ϫ T cells, both from decreased growth in vitro, and the ability of strong stimuli, like anti-CD3 plus anti-CD28 or PMA plus ionomycin, to minimize the difference in IFN-␥ production between wild-type and Stat4Ϫ/Ϫ Th1 cells (30). Although we observed binding to a number of STAT4-bound TCR signaling genes in our dataset, we only identified one, Lcp2 en- coding SLP-76, that showed dependence on STAT4 for expression in Th1 cells. We did not observe decreased expression of Lcp2 in Stat4Ϫ/Ϫ naive T cells or Th2 cells (data not shown). As SLP-76 plays a central role in TCR signaling (29), multiple pathways could be affected. We demonstrated that anti-CD3-induced ERK activa- tion was diminished in Stat4Ϫ/Ϫ Th1 cells. However, activation of downstream signaling molecules using PMA and ionomycin by- passes proximal signaling molecules and minimized the differ- ences in production between wild-type and Stat4Ϫ/Ϫ Th1 cells. Thus, Stat4Ϫ/Ϫ T cells not only have a defect in the acqui- sition of the Th1 phenotype, but also in T cell activation of cells differentiated in a Th1-promoting environment. FIGURE 7. Classification of STAT4 target genes. STAT4 target genes Several important features of STAT4 binding vs gene regulation Downloaded from are clustered by function (receptors, cytokines) or functional subset (Th1, were also realized in this study including the lack of correlation TCR signaling). The Th1 cluster contains a subcluster of IL-18R-associ- between the intensity of binding and the fold-induction of gene ated genes and includes receptors and cytokines that are color-coded with expression. Although one might expect that more binding would other clusters. Genes are classified as STAT4-dependent, -independent, or result in greater transactivation, there are several reasons that may undetermined in relation to acute IL-12 restimulation. explain a disconnect between binding and gene induction. First, the context of the promoter may dictate activity, demonstrating outside of the promoter regions analyzed in this study, the latter that even in the context of a consensus-binding site, binding is not http://www.jimmunol.org/ highlighting the limitation of a promoter- vs a genome-wide ChIP- the sole predictor of biological function. The focused time frame of on-chip. Regardless, Etv5 and Hlx1 are clearly STAT4-dependent our analysis might prevent observing induction of genes that re- Th1 genes with induction kinetics similar to other STAT4-depen- quire additional cofactors that were not present in IL-12-stimulated dent genes identified in the array (Fig. 6) (9). Conversely, 2 of the activated T cells. There may also be tissue-specific factors required 18 STAT4-bound Th1 genes (Fig. 4A) are not dependent on for STAT4 function to regulate these genes in other cell types. STAT4 for expression in Th1 cells, at least under standard in vitro Although no function for STAT4 in testis has been determined, we differentiation conditions. The Th1 lineage-promoting factor T-bet found 113 genes enriched in a testis microarray shared with the is also partially dependent upon STAT4 (9), though Tbx21 was not STAT4-bound dataset (Table S2C) that are unlikely to be induced by guest on October 1, 2021 identified in the STAT4 ChIP-on-chip dataset. As we recently in T cells. Second, the basal transcription of the gene may deter- demonstrated, many of the genes in the Th1 program are depen- mine the sensitivity of the gene to induction. We demonstrated that dent on both STAT4 and T-bet, including at least 7 of the 18 of the genes we tested where IL-12 had minimal induction, all had STAT4-bound Th1 genes (Fig. 4A and Table S3). The T-bet-de- high expression, compared with the control gene (Fig. 6C). Fi- pendence of all the genes in the STAT4-bound set has not been nally, STAT4 may bind to redundant consensus sites that mediate determined but these preliminary data suggest that there is at least gene activation by other STAT proteins. For example, Irf1 was a 30% overlap in the target genes of these two factors. bound by STAT4 but was not induced by IL-12. In contrast, IFN- Although STAT4 is clearly required for the establishment of the ␥/STAT1 induce Irf1 in a number of cell types. Similar results Th1 genetic program, how it mediates programming is still un- were observed for T-bet targets, where T-bet was found to bind but clear. STAT4 binds to target loci including Ifng, Hlx1, and Il18r1, not regulate gene targets (33). and mediates recruitment of Brg1-containing complexes, induces The benefit of genome-scale experiments is the opportunity to histone acetylation, and decreases histone methylation and the as- identify clusters of genes involved in a biological process within sociation of repressive such as DNMT3a and EZH2 (6, the activated gene regulatory network that contribute to specific 8–10). All of these events contribute to increased gene transcrip- cellular functions or phenotypes. Apart from the Th1 cluster, we tion and programming. In this report we identified distinct sets of identified a TCR signaling gene (Lcp2) that STAT4 programs for genes that are either transiently induced or have sustained induc- increased Ag receptor sensitivity and an IL-18 response cluster tion. Moreover, there was a correlation between sustained gene (Il18r1, Il18rap, Gadd45b, Gadd45g, Myd88) (Fig. 7) that sup- induction and programmed expression in Th1 cells. We have pre- ports previous observations on the requirement for STAT4 in viously analyzed an example of each of these gene categories; IL-18 sensitivity (28). A large number of IFN-induced genes were Il2ra is transiently induced, while Il18r1 is programmed for ex- also identified (Fig. 7), though the genes examined were not in- pression in Th1 cells (8, 23). At this point it is not clear whether duced to the same levels reported for IFN-␥ stimulation in other there are specific chromatin modifications that distinguish transient cell types (34). Recently, two groups have analyzed high-through- from sustained induction. However, having established many of put data from STAT1 ChIP (14, 35). Although these experiments the STAT4-dependent events that occur during Th1 differentiation were done in cultured cells (HeLa S3 or NIH3T3), and STAT1 has (9), and finding a set of genes, including Il2ra, that is only tran- differing spatial and temporal specificities of expression from siently induced, it will be possible to determine specific factors or STAT4, the proportion of chromatin immunoprecipitated STAT1 histone modifications that distinguish the effects of STAT4 at var- target sequences that contain a match to the consensus-binding ious gene promoters. sequences was similar to our studies. One of the subsets of genes identified in this study was involved In this report, using a combination of in silico and in vivo tech- in TCR signaling. Earlier reports had suggested that TCR signaling niques, we have identified STAT4 target genes in primary T cells. The Journal of Immunology 3847

Subsets of STAT4 targets mediate specific functions in Th1 cells. 15. Mathur, A. N., H. C. Chang, D. G. Zisoulis, R. Kapur, M. L. Belladonna, Analysis suggests that STAT4 target genes programmed for Th1 G. S. Kansas, and M. H. Kaplan. 2006. T-bet is a critical determinant in the instability of the IL-17-secreting T-helper phenotype. 108: 1595–1601. expression have distinct activation kinetics, suggesting that it may 16. Labhart, P., S. Karmakar, E. M. Salicru, B. S. Egan, V. Alexiadis, B. W. be possible, using bioinformatics analysis, to identify genes that O’Malley, and C. L. Smith. 2005. Identification of target genes in breast cells directly regulated by the SRC-3/AIB1 coactivator. Proc. Natl. Acad. Sci. play a role in inflammatory cell development and function. USA 102: 1339–1344. 17. Rogge, L., E. Bianchi, M. Biffi, E. Bono, S. Y. Chang, H. Alexander, C. Santini, G. Ferrari, L. Sinigaglia, M. Seiler, et al. 2000. Transcript imaging of the devel- Acknowledgments opment of human T helper cells using oligonucleotide arrays. Nat. Genet. 25: We thank C. Short for technical assistance and H. Nakshatri, C. Y. Lin, and 96–101. P. Labhart for review of this manuscript. 18. Watford, W. T., B. D. Hissong, L. R. Durant, H. Yamane, L. M. Muul, Y. Kanno, C. M. Tato, H. L. Ramos, A. E. Berger, L. Mielke, et al. 2008. Tpl2 regulates T cell -␥ production and host resistance to Toxoplasma gon- Disclosures dii. J. Exp. Med. 205: 2803–2812. 19. Bailey, T. L., and C. Elkan. 1994. Fitting a mixture model by expectation - The authors have no financial conflict of interest. imization to discover motifs in biopolymers. Proc. Int. Conf. Intell. Syst. Mol. Biol. 2: 28–36. References 20. Crooks, G. E., G. Hon, J. M. Chandonia, and S. E. Brenner. 2004. WebLogo: a sequence logo generator. Genome Res. 14: 1188–1190. 1. Kaplan, M. H. 2005. STAT4: a critical regulator of inflammation in vivo. Im- 21. Beissbarth, T., and T. P. Speed. 2004. GOstat: find statistically overrepresented munol. Res. 32: 231–241. Gene Ontologies within a group of genes. Bioinformatics 20: 1464–1465. 2. Murphy, K. M., and S. L. Reiner. 2002. The lineage decisions of helper T cells. 22. Szabo, S. J., A. S. Dighe, U. Gubler, and K. M. Murphy. 1997. Regulation of the Nat. Rev. Immunol. 2: 933–944. ␤ interleukin (IL)-12R 2 subunit expression in developing T helper 1 (Th1) and 3. Robertson, M. J., H. C. Chang, D. Pelloso, and M. H. Kaplan. 2005. Impaired Th2 cells. J. Exp. Med. 185: 817–824. ␥ interferon- production as a consequence of STAT4 deficiency after autologous 23. O’Sullivan, A., H. C. Chang, Q. Yu, and M. H. Kaplan. 2004. STAT4 is required hematopoietic stem cell transplantation for . Blood 106: 963–970. for interleukin-12-induced chromatin remodeling of the CD25 . J. Biol. Downloaded from 4. Remmers, E. F., R. M. Plenge, A. T. Lee, R. R. Graham, G. Hom, T. W. Behrens, Chem. 279: 7339–7345. P. I. de Bakker, J. M. Le, H. S. Lee, F. Batliwalla, et al. 2007. STAT4 and the risk 24. Xu, X., Y.-L. Sun, and T. Hoey. 1996. Cooperative DNA binding and sequence- of and systemic erythematosus. N. Engl. J. Med. 357: selective recognition conferred by the STAT amino-terminal domain. Science 977–986. 273: 794–797. 5. Nguyen, K. B., W. T. Watford, R. Salomon, S. R. Hofmann, G. C. Pien, 25. Sandelin, A., W. Alkema, P. Engstrom, W. W. Wasserman, and B. Lenhard. A. Morinobu, M. Gadina, J. J. O’Shea, and C. A. Biron. 2002. Critical role for 2004. JASPAR: an open-access database for eukaryotic transcription factor bind- STAT4 activation by type 1 in the interferon-␥ response to viral ing profiles. Nucleic Acids Res. 32: D91–94.

infection. Science 297: 2063–2066. 26. Pesu, M., L. Muul, Y. Kanno, and J. J. O’Shea. 2006. Proprotein convertase furin http://www.jimmunol.org/ 6. Zhang, F., and M. Boothby. 2006. T helper type 1-specific Brg1 recruitment and is preferentially expressed in T helper 1 cells and regulates interferon ␥. Blood remodeling of positioned at the IFN-␥ promoter are Stat4 depen- 108: 983–985. dent. J. Exp. Med. 203: 1493–1505. 27. Lu, B., H. Yu, C. Chow, B. Li, W. Zheng, R. J. Davis, and R. A. Flavell. 2001. 7. Letimier, F. A., N. Passini, S. Gasparian, E. Bianchi, and L. Rogge. 2007. Chro- GADD45␥ mediates the activation of the p38 and JNK MAP kinase pathways matin remodeling by the SWI/SNF-like BAF complex and STAT4 activation and cytokine production in effector TH1 cells. 14: 583–590. synergistically induce IL-12R␤2 expression during human Th1 cell differentia- 28. Lawless, V. A., S. Zhang, O. N. Ozes, H. A. Bruns, I. Oldham, T. Hoey, tion. EMBO J. 26: 1292–1302. M. J. Grusby, and M. H. Kaplan. 2000. Stat4 regulates multiple components of 8. Yu, Q., V. T. Thieu, and M. H. Kaplan. 2007. Stat4 limits DNA methyltransferase IFN-␥-inducing signaling pathways. J. Immunol. 165: 6803–6808. recruitment and DNA methylation of the IL-18R␣ gene during Th1 differentia- 29. Smith-Garvin, J. E., G. A. Koretzky, and M. S. Jordan. 2009. T cell activation. tion. EMBO J. 26: 2052–2060. Annu. Rev. Immunol. 27: 591–619. 9. Thieu, V. T., Q. Yu, H. C. Chang, N. Yeh, E. T. Nguyen, S. Sehra, and 30. Afkarian, M., J. R. Sedy, J. Yang, N. G. Jacobson, N. Cereb, S. Y. Yang,

M. H. Kaplan. 2008. Signal transducer and activator of transcription 4 is required T. L. Murphy, and K. M. Murphy. 2002. T-bet is a STAT1-induced regulator of by guest on October 1, 2021 for the transcription factor T-bet to promote T helper 1 cell-fate determination. IL-12R expression in naive CD4ϩ T cells. Nat. Immunol. 3: 549–557. Immunity 29: 679–690. 31. Chang, S., and T. M. Aune. 2005. Histone hyperacetylated domains across the 10. Yu, Q., H. C. Chang, A. N. Ahyi, and M. H. Kaplan. 2008. Transcription factor- IFN␥ gene region in natural killer cells and T cells. Proc. Natl. Acad. Sci. USA dependent chromatin remodeling of Il18r1 during Th1 and Th2 differentiation. 102: 17095–17100. J. Immunol. 181: 3346–3352. 32. Pesu, M., W. T. Watford, L. Wei, L. Xu, I. Fuss, W. Strober, J. Andersson, 11. Hoey, T., S. Zhang, N. Schmidt, Q. Yu, S. Ramchandani, X. Xu, L. K. Naeger, E. M. Shevach, M. Quezado, N. Bouladoux, et al. 2008. T-cell-expressed pro- Y. L. Sun, and M. H. Kaplan. 2003. Distinct requirements for the naturally oc- protein convertase furin is essential for maintenance of peripheral immune tol- curring splice forms Stat4␣ and Stat4␤ in IL-12 responses. EMBO J. 22: erance. Nature 455: 246–250. 4237–4248. 33. Beima, K. M., M. M. Miazgowicz, M. D. Lewis, P. S. Yan, T. H. Huang, and 12. Lund, R. J., Z. Chen, J. Scheinin, and R. Lahesmaa. 2004. Early target genes of A. S. Weinmann. 2006. T-bet binding to newly identified target gene promoters IL-12 and STAT4 signaling in Th cells. J. Immunol. 172: 6775–6782. is cell type-independent but results in variable context-dependent functional ef- 13. Ren, B., F. Robert, J. J. Wyrick, O. Aparicio, E. G. Jennings, I. Simon, fects. J. Biol. Chem. 281: 11992–12000. J. Zeitlinger, J. Schreiber, N. Hannett, E. Kanin, et al. 2000. Genome-wide lo- 34. Ramana, C. V., M. P. Gil, R. D. Schreiber, and G. R. Stark. 2002. Stat1-depen- cation and function of DNA binding proteins. Science 290: 2306–2309. dent and -independent pathways in IFN-␥-dependent signaling. Trends Immunol. 14. Robertson, G., M. Hirst, M. Bainbridge, M. Bilenky, Y. Zhao, T. Zeng, 23: 96–101. G. Euskirchen, B. Bernier, R. Varhol, A. Delaney, et al. 2007. Genome-wide 35. Hartman, S. E., P. Bertone, A. K. Nath, T. E. Royce, M. Gerstein, S. Weissman, profiles of STAT1 DNA association using chromatin immunoprecipitation and and M. Snyder. 2005. Global changes in STAT target selection and transcription massively parallel . Nat. Methods 4: 651–657. regulation upon interferon treatments. Genes Dev. 19: 2953–2968. Supplementary Table Legends

Supplementary Table 1. Curated list of STAT4 target genes. The curated list of STAT4 target genes, defined as genes that demonstrated a peak intensity of greater than or equal to 4 and were present in both data replicates) are shown sorted from highest to lowest peak value. Columns indicate Gene ID, location, the peak value from chip analysis, gene name, alternate gene names and the full name of the gene.

Supplementary Table 2. Intersect of STAT4 target genes and public databases. A, List of genes that are in the intersect of STAT4 target genes from the ChIP- chip dataset in this report and published microarray studies of IL-12-inducible genes. B, List of genes associated with TCR signaling that was compared to the STAT4 ChIP-chip dataset. The presence or absence of the gene in the STAT4 ChIP-chip dataset is noted. C, List of genes that are in the intersect of STAT4 target genes from the ChIP-chip dataset in this report and published microarray studies of testes-specific genes. Testis-specific gene set was generated using publicly available datasets GEO (Series GSE1986). Genes that were 5-fold higher in testis than in either liver or were compared to the STAT4 ChIP- chip dataset. There was an overlap of 113 genes in the dataset including Cyct, Prm1 and Stat4.

Supplementary Table 3. Genes associated with the Th1 phenotype. To search for genes associated with the Th1 phenotype present in the STAT4 ChIP-chip dataset, we generated a list of Th1 genes that have been shown to have STAT4- or T-bet-dependence for expression in Th1 cells, based on published studies. The Table lists the genes, protein name, whether they were present in the STAT4 ChIP-chip dataset, and based on cited reports, whether the genes show STAT4- or T-bet-dependence for expression in Th1 cells. References are noted below. Of 20 STAT4-dependent genes, 6 were not identified in the ChIP-on-chip. Of 18 STAT4 ChIP-on-chip targets, 2 are not dependent upon STAT4 for expression in Th1 cells

Gene ID Chromosome Peak Value Gene Name Alternate Symbols Full Description GeneID:68550 11 32.41 1110002N22Rik FLJ22729 RIKEN cDNA 1110002N22 gene GeneID:237877 11 32.41 C130052G03Rik FRAG1, RP23-328O2.1 RIKEN cDNA C130052G03 gene GeneID:216991 11 32.41 Centa2 centaurin, alpha 2 GeneID:108954 1 29.399 Ppp1r15b CReP protein phosphatase 1, regulatory (inhibitor) subunit 15b GeneID:217340 11 29.164 Rnf157 mKIAA1917 ring finger protein 157 GeneID:240539 19 28.123 Gm336 gene model 336, (NCBI) GeneID:69369 19 28.123 1700017D01Rik RIKEN cDNA 1700017D01 gene GeneID:21838 9 28.099 Thy1 CD90, Thy-1.2, Thy1.1, Thy1.2 thymus cell antigen 1, theta GeneID:241489 2 26.698 Pde11a PDE11A1 phosphodiesterase 11A GeneID:224938 17 26.596 Pja2 MGC27629, mKIAA0438 praja 2, RING-H2 motif containing GeneID:621650 17 26.596 LOC621650 hypothetical protein LOC621650 GeneID:228139 2 25.418 P2rx3 MGC28163, P2X3 purinergic receptor P2X, -gated ion channel, 3 GeneID:20833 2 25.418 Ssrp1 T160 structure specific recognition protein 1 GeneID:54167 1 25.405 Icos AILIM, H4, Ly115 inducible T-cell co-stimulator GeneID:11569 6 24.94 Aebp2 AE binding protein 2 GeneID:232288 6 24.762 Frmd4b GOBLIN, GRSP1, mKIAA1013 FERM domain containing 4B GeneID:67921 1 24.748 2510010F15Rik RIKEN cDNA 2510010F15 gene GeneID:50880 1 24.748 Scly 9830169H08, SCL, Scly1, Scly2 selenocysteine GeneID:211253 14 24.313 Mtrf1 MGC19099 mitochondrial translational 1 GeneID:66897 14 24.313 Narg1l NMDA receptor regulated 1-like GeneID:20318 4 23.978 Sdf4 stromal cell derived factor 4 GeneID:22163 4 23.978 Tnfrsf4 ACT35, CD134, Ly-70, TXGP1L receptor superfamily, member 4 GeneID:194357 6 23.854 Moxd2 monooxygenase, DBH-like 2 GeneID:621968 6 23.854 LOC621968 similar to T-cell receptor beta chain V region E1 precursor GeneID:232717 6 23.854 1700020H15 hypothetical protein 1700020H15 GeneID:71340 13 23.828 Riok1 Ad034, MGC7300 RIO kinase 1 (yeast) GeneID:50780 4 23.308 Rgs3 C2PA-RGS3, C2pa, PDZ-RGS3, RGS3Sregulator of G-protein signaling 3 GeneID:214106 4 23.308 4933430I17Rik bN189G18.7 RIKEN cDNA 4933430I17 gene GeneID:20850 11 23.205 Stat5a STAT5 signal transducer and activator of transcription 5A GeneID:317758 6 23.144 Gimap9 A630002K24 GTPase, IMAP family member 9 GeneID:107526 6 23.144 Gimap4 IMAP4, MGC11734, mIAN1 GTPase, IMAP family member 4 GeneID:14744 12 23.117 Gpr65 Dig1, TDAG8 G-protein coupled receptor 65 GeneID:73212 5 22.748 3110082I17Rik RIKEN cDNA 3110082I17 gene GeneID:320197 5 22.748 D830046C22Rik RIKEN cDNA D830046C22 gene GeneID:80290 5 22.748 Gpr146 MGC7035, PGR8 -coupled receptor 146 GeneID:20848 11 22.431 Stat3 Aprf signal transducer and activator of transcription 3 GeneID:18439 5 22.406 P2rx7 purinergic receptor P2X, ligand-gated ion channel, 7 GeneID:13640 17 22.34 Efna5 AL-1, EFL-5, Ephrin-A5, LERK-7, RAGSephrin A5 GeneID:16174 1 21.987 Il18rap interleukin 18 receptor accessory protein GeneID:18761 2 21.785 Prkcq PKC-0, PKC-theta C, theta GeneID:242125 3 21.76 BC037703 MGC47256 cDNA sequence BC037703 GeneID:16145 11 21.722 Igtp induced GTPase GeneID:54396 11 21.722 Iigp2 GTPI interferon inducible GTPase 2 GeneID:21819 15 21.357 Tgn thyroglobulin GeneID:20491 15 21.357 Sla Slap, Slap-1 src-like adaptor GeneID:72077 9 21.055 Gcnt3 glucosaminyl (N-acetyl) 3, mucin type GeneID:381319 1 21.023 9130211I03Rik Snft RIKEN cDNA 9130211I03 gene GeneID:22035 3 20.91 Tnfsf10 APO-2L, Ly81, TL2 tumor necrosis factor (ligand) superfamily, member 10 GeneID:13350 15 20.889 Dgat1 Dgat diacylglycerol O-acyltransferase 1 GeneID:170729 15 20.889 Scrt1 scratch homolog 1, protein (Drosophila) GeneID:626334 15 20.889 LOC626334 hypothetical protein LOC626334 GeneID:628281 13 20.884 LOC628281 hypothetical protein LOC628281 GeneID:20723 13 20.884 Serpinb9 CAP-3, CAP3, PI-9, PI9, ovalbumin (or cysteine) peptidase inhibitor, clade B, member 9 GeneID:18550 7 20.877 Furin PACE, SPC1 furin (paired basic cleaving ) GeneID:110197 16 20.696 Dgkg 90kDa, mKIAA4131 diacylglycerol kinase, gamma GeneID:69863 4 20.277 1810054D07Rik RP23-17O21.2 RIKEN cDNA 1810054D07 gene GeneID:14468 3 20.274 Gbp1 5830475C06, Mag-1 guanylate nucleotide binding protein 1 GeneID:14469 3 20.274 Gbp2 guanylate nucleotide binding protein 2 GeneID:545378 1 19.708 Sh2d1c EAT-2B EAT-2-related transducer GeneID:16642 6 19.386 Klrc2 NKG2C killer cell lectin-like receptor subfamily C, member 2 GeneID:215418 9 18.863 Axud1 URAX1, taip-3 AXIN1 up-regulated 1 GeneID:74052 9 18.863 ttc21a tetratricopeptide repeat domain 21A GeneID:52392 1 18.716 D1Ertd622e DNA segment, Chr 1, ERATO Doi 622, expressed GeneID:269941 7 18.587 Chsy1 KIAA0990, mKIAA0990 carbohydrate (chondroitin) synthase 1 GeneID:245666 X 17.973 Iqsec2 KIAA0522, mKIAA0522 IQ motif and Sec7 domain 2 GeneID:20591 X 17.973 Jarid1c mKIAA0234 jumonji, AT rich interactive domain 1C (Rbp2 like) GeneID:16970 6 17.874 Lrmp D6Int3, D6Int4, D6Int5, D6Int7, D6Int8,lymphoid-restricted Jaw1 GeneID:20347 9 17.725 Sema3b LUCA-1, SemA, sema5, semaV sema-Ig domains, short basic domain, secreted, (semaphorin) 3B GeneID:14678 9 17.725 Gnai2 Galphai2 guanine nucleotide binding protein, alpha inhibiting 2 GeneID:17874 9 17.615 Myd88 myeloid differentiation primary response gene 88 GeneID:113868 9 17.615 Acaa1 Acaa, MGC7090, PTL acetyl-Coenzyme A acyltransferase 1 GeneID:19057 14 17.491 Ppp3cc protein phosphatase 3, catalytic subunit, gamma isoform GeneID:20449 6 17.383 St8sia1 9330109E03Rik, GD3S, Sia-T ST8 alpha-N-acetyl-neuraminide alpha-2,8- 1 GeneID:66367 7 17.335 2310022A10Rik RIKEN cDNA 2310022A10 gene GeneID:18538 2 17.12 Pcna proliferating cell nuclear antigen GeneID:110911 2 17.12 Cds2 CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 GeneID:100273 4 17.107 Osbpl9 MGC30367, MGC38458, ORP-9 oxysterol binding protein-like 9 GeneID:72512 18 17.008 2610307O08Rik RIKEN cDNA 2610307O08 gene GeneID:58179 6 16.975 Klrc3 Nkg2e killer cell lectin-like receptor subfamily C, member 3 GeneID:71805 8 16.875 Nup93 93 GeneID:20497 8 16.875 Slc12a3 NCC, NCCT, TSC solute carrier family 12, member 3 GeneID:75665 5 16.856 2210403N09Rik RIKEN cDNA 2210403N09 gene GeneID:107094 19 16.782 AA408556 mKIAA0690 expressed sequence AA408556 GeneID:226182 19 16.746 Taf5 TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor GeneID:99237 2 16.704 Tm9sf4 B930079E06, mKIAA0255 transmembrane 9 superfamily protein member 4 GeneID:229898 3 16.635 Gbp5 guanylate nucleotide binding protein 5 GeneID:621548 3 16.635 LOC621548 similar to HYPOTHETICAL PROTEIN ORF-1137 GeneID:226519 1 16.352 Lamc1 Lamb2 , gamma 1 GeneID:320201 11 16.3 4930412M03Rik RIKEN cDNA 4930412M03 gene GeneID:17690 5 16.3 Msi1h Msi1, Musahi1, m-Msi-1 Musashi homolog 1(Drosophila) GeneID:170799 10 16.253 Plekhk1 RTKN2 pleckstrin homology domain containing, family K member 1 GeneID:225884 19 16.188 BC021614 MGC37914 cDNA sequence BC021614 GeneID:67501 16 16.173 2610529H08Rik C3orf6 RIKEN cDNA 2610529H08 gene GeneID:80291 5 16.158 BC003324 MGC7036 cDNA sequence BC003324 GeneID:606492 5 16.158 LOC606492 Ppia peptidylprolyl A GeneID:76167 5 16.158 6330548G22Rik RIKEN cDNA 6330548G22 gene GeneID:75695 5 16.158 2900002H16Rik FLJ39378 RIKEN cDNA 2900002H16 gene GeneID:14421 10 16.079 Galgt1 Gal-NAc-T, GalNAc-T, GalNAcT UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramide-beta-1, 4-N-acetylgalactosaminyltransferase GeneID:12380 13 15.935 Cast GeneID:622204 13 15.935 LOC622204 similar to Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing, Single-strand binding protein) (hnRNP core protein A1) (HDP-1) GeneID:16211 11 15.867 Kpnb1 IPOB karyopherin () beta 1 GeneID:60505 3 15.867 Il21 interleukin 21 GeneID:12508 3 15.846 Cd53 Ox-44, Tspan25 CD53 antigen GeneID:54353 6 15.793 Scap2 RA70, SKAP-HOM, Saps, mSKAP55Rsrc family associated phosphoprotein 2 GeneID:22640 8 15.675 Zfp1 mkr-1 zinc finger protein 1 GeneID:382112 9 15.365 Gm1127 gene model 1127, (NCBI) GeneID:71449 1 15.345 5630401D24Rik RIKEN cDNA 5630401D24 gene GeneID:435626 1 15.225 F930048N03Rik RIKEN cDNA F930048N03 gene GeneID:12765 1 15.225 Il8rb CD128, CDw128, CXCR2, IL-8Rh, IL-8rb,interleukin IL8RA, 8 receptor mIL-8RH, beta GeneID:57911 11 15.201 Gsdm1 Gsdm, H312E gasdermin 1 GeneID:22123 11 15.201 Psmd3 AntP91a, D11Bwg1349e, Psd3 (prosome, macropain) 26S subunit, non-ATPase, 3 GeneID:71982 6 15.147 Snx10 sorting nexin 10 GeneID:54711 2 15.045 Plagl2 mKIAA0198 pleiomorphic adenoma gene-like 2 GeneID:140484 2 15.045 Pofut1 O-FucT-1, mKIAA0180 protein O- 1 GeneID:622054 18 14.931 LOC622054 hypothetical protein LOC622054 GeneID:21417 18 14.931 Zfhx1a AREB6, BZP, MEB1, ZEB, Zfhep, Zfx1ha,zinc finger [delta]EF1 1a GeneID:19650 2 14.85 Rbl1 PRB1, p107 -like 1 (p107) GeneID:56045 2 14.85 Samhd1 Mg11 SAM domain and HD domain, 1 GeneID:12557 4 14.821 Cdh17 HPT-1, HPT-1/LI cadherin 17 GeneID:16569 2 14.804 Kif3b mKIAA0359 family member 3B GeneID:74901 8 14.752 Kbtbd11 mKIAA0711 kelch repeat and BTB (POZ) domain containing 11 GeneID:100855 5 14.742 Tbc1d14 mKIAA1322 TBC1 domain family, member 14 GeneID:72900 17 14.741 Ndufv2 NADH dehydrogenase (ubiquinone) flavoprotein 2 GeneID:68767 17 14.741 ORF19 open 19 GeneID:66632 2 14.424 5730421E18Rik RIKEN cDNA 5730421E18 gene GeneID:12040 9 14.388 Bckdhb branched chain ketoacid dehydrogenase E1, beta polypeptide GeneID:619606 5 14.29 LOC619606 skip GeneID:215387 2 14.281 Brrn1 HCAP-H, MGC38424, mKIAA0074 barren homolog (Drosophila) GeneID:73338 2 14.281 1700041B20Rik RIKEN cDNA 1700041B20 gene GeneID:66713 11 14.265 Actr2 Arp2, MGC36604 ARP2 -related protein 2 homolog (yeast) GeneID:217410 12 14.246 Trib2 TRB-2 tribbles homolog 2 (Drosophila) GeneID:432636 12 14.246 LOC432636 hypothetical gene supported by AK045700 GeneID:80287 15 14.193 Apobec3 Arp3, CEM15, D730005E14Rik, MGC7002apolipoprotein B editing complex 3 GeneID:74732 10 14.037 Stx11 syntaxin 11 GeneID:16184 2 13.991 Il2ra CD25 interleukin 2 receptor, alpha chain GeneID:327957 11 13.952 LOC327957 similar to DTFT5783 GeneID:50783 8 13.828 Lsm4 LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) GeneID:108689 19 13.812 Obfc1 oligonucleotide/oligosaccharide-binding fold containing 1 GeneID:140859 11 13.802 Nek8 NIMA (never in mitosis gene a)-related expressed kinase 8 GeneID:68385 11 13.802 0610007A15Rik RP23-185A18.3 RIKEN cDNA 0610007A15 gene GeneID:268449 11 13.802 Rpl23a MDA20, MGC36820 L23a GeneID:12305 17 13.784 Ddr1 6030432F18, CD167a, PTK3A discoidin domain receptor family, member 1 GeneID:21753 6 13.745 Tes TESS, Tes1, Tes2, testin2 testis derived transcript GeneID:232599 6 13.745 B930018B01 hypothetical protein B930018B01 GeneID:229900 3 13.726 9830147J24Rik RIKEN cDNA 9830147J24 gene GeneID:18569 19 13.69 Pdcd4 MA-3, TIS programmed cell death 4 GeneID:330260 6 13.636 Pon2 MGC68232 paraoxonase 2 GeneID:628494 7 13.62 LOC628494 hypothetical protein LOC628494 GeneID:627906 7 13.62 LOC627906 similar to of outer mitochondrial membrane 7 homolog GeneID:67003 7 13.62 Uqcrc2 4930408O21Rik ubiquinol cytochrome c reductase core protein 2 GeneID:12934 14 13.598 Dpysl2 DRP2, Musunc33, TOAD-64, Ulip2 -like 2 GeneID:15216 13 13.533 Hfe MR2 hemochromatosis GeneID:19073 10 13.401 Prg1 Prg proteoglycan 1, secretory granule GeneID:16874 2 13.338 Lhx6 LIM homeobox protein 6 GeneID:18030 13 13.284 Nfil3 E4BP4 nuclear factor, , regulated GeneID:14762 12 13.229 Gpr33 G protein-coupled receptor 33 GeneID:16478 8 13.211 Jund1 MGC6245 Jun proto- related gene d1 GeneID:235283 9 13.144 A930008A22Rik 3222402H23, KIAA1201, mKIAA1201RIKEN cDNA A930008A22 gene GeneID:102626 9 13.143 Mapkapk3 3PK, MAPKAP3, MapkKapk3 mitogen-activated protein kinase-activated protein kinase 3 GeneID:12700 9 13.143 Cish CIS1, Cis, F17, F23 cytokine inducible SH2-containing protein GeneID:21454 17 13.107 Tcp1 CCT, Ccta, TRic, c-cpn t-complex protein 1 GeneID:67681 17 13.107 Mrpl18 HSPC071, MRP-L18 mitochondrial ribosomal protein L18 GeneID:240023 17 13.107 Gm313 gene model 313, (NCBI) GeneID:19296 15 13.103 Pvt1 plasmacytoma variant translocation 1 GeneID:14682 19 13.074 Gnaq Galphaq, Gq guanine nucleotide binding protein, alpha q polypeptide GeneID:26938 3 13.073 St6galnac5 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 GeneID:11776 10 13.068 Ap3d1 Ap3d, mBLVR1, mocha adaptor-related 3, delta 1 subunit GeneID:208266 10 13.068 Dot1l A630076O07, Dot1, mDot1 DOT1-like, methyltransferase (S. cerevisiae) GeneID:319503 12 13.032 9930038B18Rik RIKEN cDNA 9930038B18 gene GeneID:22780 11 12.992 Zfpn1a3 Znfn1a3 zinc finger protein, subfamily 1A, 3 (Aiolos) GeneID:69376 11 12.992 Zpbp2 2610022C02Rik, ZPBPL zona pellucida binding protein 2 GeneID:216850 11 12.946 Jmjd3 RP23-5O23.5, mKIAA0346 jumonji domain containing 3 GeneID:16477 8 12.884 Junb Jun-B oncogene GeneID:21672 8 12.884 Prdx2 Band-8, NkefB, PRP, PrxII, TDX1, TPx,peroxiredoxin TPx-B, TR, 2 TSA, Torin GeneID:170833 8 12.884 Hook2 MGC28586 hook homolog 2 (Drosophila) GeneID:243963 7 12.869 Zfp473 4932441F21 zinc finger protein 473 GeneID:101568 7 12.869 Vrk3 vaccinia related kinase 3 GeneID:208449 19 12.849 Tmem23 MGC30540, MOB, SMS1, Sor1 transmembrane protein 23 GeneID:104215 17 12.819 Rhoq TC10A, Tc10 ras homolog gene family, member Q GeneID:75796 8 12.8 Cdyl2 chromodomain protein, Y chromosome-like 2 GeneID:108078 6 12.737 Olr1 LOX-1, SR-EI, Scare1 oxidized low density lipoprotein (lectin-like) receptor 1 GeneID:330428 6 12.737 D630042F21Rik RIKEN cDNA D630042F21 gene GeneID:21391 6 12.727 Tbxas1 CYP5, CYP5A1, THAS, TS, TXAS, TXSthromboxane A synthase 1, platelet GeneID:210126 16 12.714 Lpp D630048H16 LIM domain containing preferred translocation partner in lipoma GeneID:624582 16 12.714 LOC624582 similar to mortality factor 4 like 1 GeneID:100604 5 12.704 Lrrc8c AD158, fad158 rich repeat containing 8 family, member C GeneID:67457 19 12.679 1200004M23Rik RIKEN cDNA 1200004M23 gene GeneID:14345 9 12.66 Fut4 FAL, FucT-IV, LeX fucosyltransferase 4 GeneID:69325 9 12.66 1700012B09Rik RIKEN cDNA 1700012B09 gene GeneID:16197 15 12.609 Il7r CD127 interleukin 7 receptor GeneID:107702 7 12.605 Rnh1 RNH ribonuclease/angiogenin inhibitor 1 GeneID:546015 7 12.605 LOC546015 similar to GeneID:23806 9 12.559 Arih1 ARI, Ari1, HARI, HHARI, UBCH7BP, ariadneUIP77 -conjugating enzyme E2 binding protein homolog 1 (Drosophila) GeneID:29809 1 12.525 Rabgap1l 8430421H08Rik, HHL, Hh1, mKIAA0471RAB GTPase activating protein 1-like GeneID:15978 10 12.448 Ifng IFN-g, IFN-gamma interferon gamma GeneID:20849 1 12.406 Stat4 signal transducer and activator of transcription 4 GeneID:20846 1 12.406 Stat1 signal transducer and activator of transcription 1 GeneID:627127 8 12.395 LOC627127 similar to open reading frame 50 GeneID:74187 8 12.395 Katnb1 KAT katanin p80 (WD40-containing) subunit B 1 GeneID:14011 6 12.38 Etv6 Tel ets variant gene 6 (TEL oncogene) GeneID:270152 9 12.258 Amica1 AMICA, Crea7, MGC61025 adhesion molecule, interacts with CXADR antigen 1 GeneID:54630 X 12.201 Lmo6 Sfc16 LIM domain only 6 GeneID:18824 X 12.201 Plp2 mIMA4 proteolipid protein 2 GeneID:54634 X 12.201 Pdzx Sfc18 PDZ domain containing, GeneID:54483 16 12.188 Mefv FMF, TRIM20, pyrin Mediterranean fever GeneID:620395 16 12.188 LOC620395 similar to Teratocarcinoma-derived growth factor precursor (Epidermal growth factor-like Cripto protein) (Cripto growth factor) GeneID:18195 11 12.098 Nsf SKD2 N-ethylmaleimide sensitive fusion protein GeneID:11841 11 12.098 Arf2 ADP-ribosylation factor 2 GeneID:72567 10 12.076 Bclaf1 Btf, MGC30519, mKIAA0164 BCL2-associated transcription factor 1 GeneID:71804 10 12.076 2610016C23Rik RIKEN cDNA 2610016C23 gene GeneID:101187 6 12.063 Parp11 HIN1L poly (ADP-ribose) polymerase family, member 11 GeneID:231507 5 11.963 Plac8 C15 placenta-specific 8 GeneID:76421 5 11.958 1700028K03Rik RIKEN cDNA 1700028K03 gene GeneID:110454 15 11.939 Ly6a Ly-6A.2, Ly-6A/E, Ly-6E.1, Sca-1, Sca1, TAP antigen 6 complex, locus A GeneID:13870 7 11.891 Ercc1 excision repair cross-complementing rodent repair deficiency, complementation group 1 GeneID:20511 2 11.861 Slc1a2 GLT-1, GLT1, MGLT1 solute carrier family 1 (glial high affinity glutamate transporter), member 2 GeneID:22381 X 11.847 Wbp5 WW domain binding protein 5 GeneID:266645 1 11.806 Acmsd amino carboxymuconate semialdehyde decarboxylase GeneID:72949 1 11.806 Ccnt2 T2 GeneID:16364 13 11.713 Irf4 LSIRF interferon regulatory factor 4 GeneID:383075 15 11.701 Gm1242 gene model 1242, (NCBI) GeneID:72747 18 11.658 2810439F02Rik RIKEN cDNA 2810439F02 gene GeneID:381157 18 11.627 AK220484 mKIAA4095 cDNA sequence AK220484 GeneID:73449 18 11.626 1700066B19Rik RIKEN cDNA 1700066B19 gene GeneID:23882 13 11.564 Gadd45g CR6, DDIT2, OIG37 growth arrest and DNA-damage-inducible 45 gamma GeneID:12053 16 11.481 Bcl6 B-cell /lymphoma 6 GeneID:622871 7 11.463 LOC622871 similar to Protein C6orf115 GeneID:623101 X 11.445 LOC623101 similar to 40S ribosomal protein S28 GeneID:258712 1 11.43 Olfr433 MOR123-1 olfactory receptor 433 GeneID:258711 1 11.43 Olfr432 MOR123-2 olfactory receptor 432 GeneID:226695 1 11.43 Ifi205 D3, D3cDNA interferon activated gene 205 GeneID:66570 15 11.411 2610019I03Rik Pane1 RIKEN cDNA 2610019I03 gene GeneID:76505 15 11.411 1500009C09Rik RIKEN cDNA 1500009C09 gene GeneID:330671 7 11.391 BC038881 MGC48196, beta4GalNAcT4 cDNA sequence BC038881 GeneID:20340 8 11.391 Glg1 CFR, CFR-1, ESL-1, MG-160, MG160golgi apparatus protein 1 GeneID:21981 12 11.354 Ppp1r13b ASPP1, Tp53bp2, Trp53bp2, p85 protein phosphatase 1, regulatory (inhibitor) subunit 13B GeneID:229687 3 11.342 Bclp2 Bclp1 like protein 2 GeneID:270035 8 11.331 D030041N04Rik 6030453H13 RIKEN cDNA D030041N04 gene GeneID:627692 8 11.331 LOC627692 similar to Wolf-Hirschhorn syndrome candidate 1-like 1 (human) GeneID:234135 8 11.331 Whsc1l1 A530023P05, NSD3 Wolf-Hirschhorn syndrome candidate 1-like 1 (human) GeneID:72181 4 11.329 2810405F18Rik RIKEN cDNA 2810405F18 gene GeneID:56066 5 11.327 Cxcl11 CXC11, H174, I-TAC, IP9, ITAC, SCYB9B,chemokine b-R1, (C-X-C betaR1 motif) ligand 11 GeneID:15945 5 11.327 Cxcl10 C7, CRG-2, INP10, IP-10, IP10, gIP-10,chemokine mob-1 (C-X-C motif) ligand 10 GeneID:50874 3 11.316 Tmod4 MTMOD, Sk-Tmod 4 GeneID:69269 3 11.316 Scnm1 sodium channel modifier 1 GeneID:217779 3 11.316 Lysmd1 MGC38837 LysM, putative peptidoglycan-binding, domain containing 1 GeneID:69769 3 11.316 Tnfaip8l2 tumor necrosis factor, alpha-induced protein 8-like 2 GeneID:73067 8 11.313 3110005G23Rik RIKEN cDNA 3110005G23 gene GeneID:69890 14 11.296 Zfp219 zinc finger protein 219 GeneID:619301 14 11.296 G630016D24Rik RIKEN cDNA G630016D24 gene GeneID:22367 12 11.273 Vrk1 51PK vaccinia related kinase 1 GeneID:435787 4 11.253 Rpl17-ps bN312B5.2 ribosomal protein L17 (Rpl17) pseudogene GeneID:12362 9 11.202 Casp1 Caspase-1, ICE caspase 1 GeneID:12363 9 11.202 Casp11 Caspase-11, ich-3 caspase 11, -related cysteine peptidase GeneID:238896 13 11.167 LOC238896 similar to CDC20-like protein form 2 GeneID:69590 13 11.167 2310016C16Rik RIKEN cDNA 2310016C16 gene GeneID:20663 12 11.137 Sos2 homolog 2 (Drosophila) GeneID:217666 12 11.137 L2hgdh MGC28775 L-2-hydroxyglutarate dehydrogenase GeneID:56404 9 11.136 Trip4 ASC-1 thyroid interactor 4 GeneID:623410 9 11.067 LOC623410 hypothetical protein LOC623410 GeneID:76459 9 11.067 Car12 carbonic anyhydrase 12 GeneID:16330 4 11.064 Inpp5b 75kDa, INPP5P inositol polyphosphate-5-phosphatase B GeneID:75062 4 11.064 Sf3a3 60kDa splicing factor 3a, subunit 3 GeneID:67344 4 11.061 1700055O19Rik RIKEN cDNA 1700055O19 gene GeneID:94092 11 11.045 Trim16 EBBP tripartite motif protein 16 GeneID:56460 7 10.992 Pkp3 3 GeneID:24058 7 10.992 AI256711 Sigirr, TIR8 expressed sequence AI256711 GeneID:71345 7 10.992 Trp53i5 Trp53 inducible protein 5 GeneID:20787 11 10.848 Srebf1 ADD-1, D630008H06, SREBP-1, SREBP-1a,sterol regulatory SREBP-1c, element SREBP1 binding factor 1 GeneID:216810 11 10.848 Tom1l2 RP23-456O18.4, Srebf1 target of myb1-like 2 (chicken) GeneID:225432 18 10.832 Rbm27 A730010I06, mKIAA1311 RNA binding motif protein 27 GeneID:65112 2 10.829 Tmepai N4wbp4, PMEPA1, STAG1 transmembrane, prostate androgen induced RNA GeneID:58203 2 10.829 Zbp1 Dlm1, mZaDLM Z-DNA binding protein 1 GeneID:67456 6 10.756 1200009B18Rik Ptx1 RIKEN cDNA 1200009B18 gene GeneID:216161 10 10.745 Stno F730040C21, Sno strawberry notch homolog (Drosophila) GeneID:545260 18 10.708 LOC545260 similar to arylsulfatase I GeneID:12322 18 10.708 Camk2a CaMKII, mKIAA0968 calcium/-dependent protein kinase II alpha GeneID:21899 5 10.688 Tlr6 toll-like receptor 6 GeneID:68303 5 10.688 9130005N14Rik RIKEN cDNA 9130005N14 gene GeneID:27007 6 10.652 Klrk1 NKG2-D, Nkg2d killer cell lectin-like receptor subfamily K, member 1 GeneID:12702 11 10.626 Socs3 CIS3, EF-10, SOCS-3, SSI-3 suppressor of cytokine signaling 3 GeneID:18810 15 10.581 Plec1 EBS1, PCN, PLTN 1 GeneID:244694 9 10.559 4932416A15 hypothetical protein 4932416A15 GeneID:622953 9 10.559 LOC622953 hypothetical protein LOC622953 GeneID:66070 9 10.559 0610040D20Rik Ed1, c11orf5, mED1 RIKEN cDNA 0610040D20 gene GeneID:68098 5 10.541 Rchy1 ARNIP, CHIMP, PRO1996, Pirh2, mARNIPring finger and CHY zinc finger domain containing 1 GeneID:207175 3 10.52 Cetn4 centrin 4 GeneID:241950 3 10.52 Gm1805 gene model 1805, (NCBI) GeneID:68259 3 10.483 Wdr56 mKIAA1374 WD repeat domain 56 GeneID:70099 3 10.483 Smc4l1 SMC4 structural maintenance of 4-like 1 (yeast) GeneID:67513 4 10.457 2610002J02Rik RIKEN cDNA 2610002J02 gene GeneID:110960 15 10.402 Tars ThrRS threonyl-tRNA synthetase GeneID:228019 2 10.393 BC004636 MGC67805, MGC7995 cDNA sequence BC004636 GeneID:621795 2 10.393 LOC621795 similar to DnaJ-like protein 2 GeneID:75763 2 10.393 4833418A01Rik RIKEN cDNA 4833418A01 gene GeneID:622044 19 10.381 LOC622044 similar to reduced expression 2 GeneID:13340 19 10.381 Slc29a2 ENT2, HNP36 solute carrier family 29 (nucleoside transporters), member 2 GeneID:246198 11 10.321 Mllt6 myeloid/lymphoid or mixed lineage-leukemia translocation to 6 homolog (Drosophila) GeneID:408065 13 10.32 Zfp456 Rslcan-13 zinc finger protein 456 GeneID:629027 13 10.32 LOC629027 hypothetical protein LOC629027 GeneID:71683 18 10.32 Gypc Cd236r GeneID:14225 2 10.318 Fkbp1a 12kDa, FKBP12, mFKBP1, mFKBP12FK506 binding protein 1a GeneID:242341 4 10.312 Atp6v0d2 V-ATPase ATPase, H+ transporting, V0 subunit D, isoform 2 GeneID:246177 11 10.184 Myo1g 1G myosin IG GeneID:328699 16 10.148 Gabrr3 gamma-aminobutyric acid (GABA) receptor, rho 3 GeneID:67014 16 10.148 Mina induced nuclear antigen GeneID:14727 10 10.12 Gp49a gp49 glycoprotein 49 A GeneID:14728 10 10.12 Lilrb4 CD85K, HM18, ILT3, LIR-5, gp49 leukocyte immunoglobulin-like receptor, subfamily B, member 4 GeneID:75410 7 10.113 Wbp7 mKIAA0304 WW domain binding protein 7 GeneID:58206 7 10.113 Zbtb32 FAXF, FAZF, PLZP, Tzfp zinc finger and BTB domain containing 32 GeneID:109637 7 10.113 Upk1a Tspan21 uroplakin 1A GeneID:331000 9 10.108 LOC331000 similar to phospholipid scramblase 1 GeneID:22038 9 10.108 Plscr1 MmTRA1a, MmTRA1b, NOR1, TRA1phospholipid scramblase 1 GeneID:75608 2 10.099 Chmp4b chromatin modifying protein 4B GeneID:22187 11 10.09 Ubb ubiquitin B GeneID:209378 2 10.071 Itih5 E130106B02 inter-alpha (globulin) inhibitor H5 GeneID:12503 1 10.059 Cd3z CD247, CD3-eta, CD3-zeta, TCRk CD3 antigen, zeta polypeptide GeneID:70797 5 10.044 Ankib1 mKIAA1386 repeat and IBR domain containing 1 GeneID:79264 5 10.044 Krit1 Krit1A, Krit1B KRIT1, containing GeneID:546714 18 10.044 LOC546714 similar to LOC435565 protein GeneID:56306 6 10 Tera teratocarcinoma expressed, serine rich GeneID:109163 6 10 3010003L21Rik RIKEN cDNA 3010003L21 gene GeneID:211948 14 9.977 E430028B21Rik 2'-PDE RIKEN cDNA E430028B21 gene GeneID:224180 16 9.963 B130050K08 hypothetical protein B130050K08 GeneID:75216 12 9.958 4930534B04Rik E230024F20 RIKEN cDNA 4930534B04 gene GeneID:257851 1 9.949 GA_x5J8B7W3B3M-30314-31216 olfactory receptor GA_x5J8B7W3B3M-30314-31216 GeneID:57248 15 9.923 Ly6i Ly-6I, Ly-6M lymphocyte antigen 6 complex, locus I GeneID:208228 10 9.907 Mobkl2a A630029F06 MOB1, Mps One Binder kinase activator-like 2A (yeast) GeneID:71564 10 9.907 9030607L17Rik RIKEN cDNA 9030607L17 gene GeneID:20933 2 9.898 Surf5 surfeit gene 5 GeneID:20930 2 9.898 Surf1 surfeit gene 1 GeneID:20935 2 9.898 Surf6 surfeit gene 6 GeneID:20934 2 9.898 Surf5-ps surfeit gene 5, pseudogene GeneID:17966 11 9.828 Nbr1 mKIAA0049 neighbor of Brca1 gene 1 GeneID:217203 11 9.828 0610008L10Rik MGC30290, MGC37887 RIKEN cDNA 0610008L10 gene GeneID:52530 11 9.789 Nola2 Nhp2 nucleolar A, member 2 GeneID:66089 11 9.789 0610039K22Rik RIKEN cDNA 0610039K22 gene GeneID:228413 2 9.745 Prrg4 TMG4 proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) GeneID:106878 18 9.703 2010002N04Rik cI-41 RIKEN cDNA 2010002N04 gene GeneID:629304 18 9.703 LOC629304 hypothetical protein LOC629304 GeneID:17184 18 9.655 Matr3 mKIAA0723 matrin 3 GeneID:67869 18 9.655 Paip2 polyadenylate-binding protein-interacting protein 2 GeneID:210711 7 9.616 1110007A13Rik MGC36596 RIKEN cDNA 1110007A13 gene GeneID:207352 7 9.616 Sec23ip Sec23 interacting protein GeneID:108150 8 9.565 Galnt7 UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7 GeneID:230787 4 9.564 BC013712 ICB-1 cDNA sequence BC013712 GeneID:21947 X 9.54 Cd40lg CD154, HIGM1, IGM, IMD3, T-BAM,CD40 TRAP ligand, gp39 GeneID:12545 5 9.523 Cdc7 muCdc7 cycle 7 (S. cerevisiae) GeneID:210004 11 9.516 6030413G23Rik B3GNTL1 RIKEN cDNA 6030413G23 gene GeneID:14194 1 9.488 Fh1 fumarate hydratase 1 GeneID:98256 1 9.488 Kmo kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) GeneID:625760 11 9.484 LOC625760 similar to ribosomal protein L18a GeneID:18413 11 9.484 Osm oncostatin M GeneID:12317 8 9.456 Calr CRT, Calregulin GeneID:546080 8 9.456 LOC546080 similar to ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 2 GeneID:66590 8 9.456 Farsla phenylalanine-tRNA synthetase-like, alpha subunit GeneID:20355 6 9.455 Sema4f sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain GeneID:236798 X 9.445 Gpr112 PGR17 G protein-coupled receptor 112 GeneID:626663 X 9.445 LOC626663 similar to G-protein coupled receptor 112 GeneID:14772 5 9.44 Gprk2l GRK, Gprk4, Grk4 G protein-coupled receptor kinase 2, groucho gene related (Drosophila) GeneID:15194 5 9.44 Hdh HD, IT15, htt, huntingtin Huntington disease gene homolog GeneID:107321 19 9.434 Lpxn LDPL leupaxin GeneID:99382 2 9.428 Abtb2 ankyrin repeat and BTB (POZ) domain containing 2 GeneID:13835 6 9.423 Epha1 Eph Eph receptor A1 GeneID:629627 14 9.407 LOC629627 similar to aspartoacylase-3 GeneID:22070 14 9.407 Tpt1 TCTP tumor protein, translationally-controlled 1 GeneID:72083 16 9.371 2410018G20Rik RIKEN cDNA 2410018G20 gene GeneID:623267 16 9.371 LOC623267 similar to ribosomal protein L30 GeneID:21822 11 9.371 Tgtp Gtp2, Mg21 T-cell specific GTPase GeneID:621855 11 9.371 LOC621855 similar to poly(rC) binding protein 2 GeneID:107221 19 9.318 Gpr120 GT01 G protein-coupled receptor 120 GeneID:77590 7 9.303 4631426J05Rik BRAG, mKIAA0598 RIKEN cDNA 4631426J05 gene GeneID:66249 11 9.282 1810003N24Rik RP23-32H19.2 RIKEN cDNA 1810003N24 gene GeneID:103784 11 9.282 AI553587 HZGJ, RP23-32H19.3 expressed sequence AI553587 GeneID:328801 17 9.25 Zfp414 AY665714, MGC49274 zinc finger protein 414 GeneID:378460 17 9.25 Pram1 PML-RAR alpha-regulated adaptor molecule 1 GeneID:110542 15 9.247 Amhr2 MISIIR, Misrii anti-Mullerian hormone type 2 receptor GeneID:66151 15 9.247 1110020C13Rik RIKEN cDNA 1110020C13 gene GeneID:545051 14 9.189 LOC545051 similar to T-CELL RECEPTOR ALPHA CHAIN C REGION GeneID:110168 14 9.173 Gpr18 G protein-coupled receptor 18 GeneID:11938 5 9.164 Atp2a2 SERCA2, mKIAA4195 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 GeneID:268749 14 9.145 Rnf31 Flj10111, Paul, mFLJ00217 ring finger protein 31 GeneID:16391 14 9.145 Isgf3g Irf-9, p48 interferon dependent positive acting transcription factor 3 gamma GeneID:207269 18 9.116 BC023105 MGC28198 cDNA sequence BC023105 GeneID:27218 1 9.094 Slamf1 4933415F16, CD150, CDw150, IPO-3signaling lymphocytic activation molecule family member 1 GeneID:74012 3 9.072 Rap2b RAP2B, member of RAS oncogene family GeneID:12960 5 9.051 Crybb1 BB1CRY crystallin, beta B1 GeneID:22022 5 9.051 Tpst2 protein-tyrosine sulfotransferase 2 GeneID:53872 2 9.039 Gpiap1 Gpiap, MMGPIP137, P137 GPI-anchored membrane protein 1 GeneID:28081 11 9.039 D11Wsu99e DNA segment, Chr 11, Wayne State University 99, expressed GeneID:276852 11 9.039 D11Wsu47e MGC30773, MGC57008 DNA segment, Chr 11, Wayne State University 47, expressed GeneID:231293 5 9.027 C130090K23Rik RIKEN cDNA C130090K23 gene GeneID:101497 7 9.01 Plekhg2 Clg, Cslg pleckstrin homology domain containing, family G (with RhoGef domain) member 2 GeneID:22695 7 9.01 Zfp36 Gos24, Nup475, TIS11D, TISII zinc finger protein 36 GeneID:67224 7 9.01 Ixl intersex-like (Drosophila) GeneID:54427 10 9.006 Dnmt3l ecat7 DNA (cytosine-5-)-methyltransferase 3-like GeneID:50723 10 9.006 Icosl B7-H2, B7RP-1, B7h, GI50, GL50, GL50-B,icos ligand ICOS-L, LICOS, Ly115l, mKIAA0653 GeneID:15894 9 8.997 Icam1 CD54, MALA-2, MGC6195 intercellular adhesion molecule GeneID:66163 9 8.997 Mrpl4 mitochondrial ribosomal protein L4 GeneID:21942 4 8.996 Tnfrsf9 4-1BB, CDw137, ILA tumor necrosis factor receptor superfamily, member 9 GeneID:57320 4 8.996 Park7 DJ-1, Dj1 Parkinson disease (autosomal recessive, early onset) 7 GeneID:17067 15 8.996 Ly6c lymphocyte antigen 6 complex, locus C GeneID:209200 16 8.983 Dtx3l deltex 3-like (Drosophila) GeneID:80285 16 8.983 Parp9 Bagl, Bal, MGC7868 poly (ADP-ribose) polymerase family, member 9 GeneID:94090 12 8.974 Trim9 mKIAA0282 tripartite motif protein 9 GeneID:225997 19 8.973 Trpm6 TRPM6/CHAK2 transient receptor potential cation channel, subfamily M, member 6 GeneID:66395 19 8.964 Ahnak DY6 AHNAK (desmoyokin) GeneID:212933 1 8.939 4732466D17Rik RIKEN cDNA 4732466D17 gene GeneID:98396 1 8.939 Slc41a1 solute carrier family 41, member 1 GeneID:238693 13 8.937 A530094I17Rik Rslcan5 RIKEN cDNA A530094I17 gene GeneID:629056 13 8.937 LOC629056 similar to zinc finger protein 181 (HHZ181) GeneID:237887 11 8.932 Slfn10 mFLJ00257 schlafen 10 GeneID:50877 7 8.927 Neu3 3 GeneID:66624 7 8.927 Spcs2 MGC60452, mKIAA0102 signal peptidase complex subunit 2 homolog (S. cerevisiae) GeneID:19342 7 8.926 Rab4b RAB4B, member RAS oncogene family GeneID:12587 7 8.926 Mia1 CD-RAP, MIA inhibitory activity 1 GeneID:53607 7 8.926 Snrpa Rnu1a-1, Rnu1a1, U1A small nuclear ribonucleoprotein polypeptide A GeneID:13730 6 8.883 Emp1 TMP epithelial membrane protein 1 GeneID:67911 13 8.869 Zfp169 zinc finger protein 169 GeneID:442803 13 8.869 A830005F24Rik RIKEN cDNA A830005F24 gene GeneID:16476 4 8.865 Jun AP-1, c-jun Jun oncogene GeneID:12211 17 8.864 Birc6 mKIAA1289 baculoviral IAP repeat-containing 6 GeneID:241732 2 8.862 Tspyl3 MGC58351 TSPY-like 3 GeneID:16205 6 8.834 Gimap1 IAP38, imap GTPase, IMAP family member 1 GeneID:317757 6 8.834 Gimap5 D630024P16 GTPase, IMAP family member 5 GeneID:23834 11 8.828 Cdc6 CDC18L cell division cycle 6 homolog (S. cerevisiae) GeneID:12515 6 8.814 Cd69 AIM, VEA CD69 antigen GeneID:606496 7 8.801 Gsk3a kinase 3 alpha GeneID:77124 7 8.801 9130221H12Rik RIKEN cDNA 9130221H12 gene GeneID:13875 7 8.801 Erf Ets2 repressor factor GeneID:235661 9 8.772 Dnclic1 LIC-1, MGC32416 , cytoplasmic, intermediate chain 1 GeneID:67213 9 8.772 Cklfsf6 chemokine-like factor super family 6 GeneID:625093 X 8.767 LOC625093 hypothetical protein LOC625093 GeneID:59026 X 8.767 Huwe1 Ib772, LASU1, Mule HECT, UBA and WWE domain containing 1 GeneID:30056 12 8.734 Timm9 Tim10a translocase of inner mitochondrial membrane 9 homolog (yeast) GeneID:76967 12 8.734 2700049A03Rik mKIAA0586 RIKEN cDNA 2700049A03 gene GeneID:620931 X 8.712 LOC620931 similar to tumor protein, translationally-controlled 1 GeneID:54646 X 8.706 Ppp1r3f Sfc15 protein phosphatase 1, regulatory (inhibitor) subunit 3F GeneID:20371 X 8.706 Foxp3 JM2, scurfin forkhead box P3 GeneID:621618 5 8.689 LOC621618 hypothetical protein LOC621618 GeneID:67238 5 8.689 2810453I06Rik Sipar RIKEN cDNA 2810453I06 gene GeneID:233276 7 8.68 Tubgcp5 GCP5, MGC38608, mKIAA1899 , gamma complex associated protein 5 GeneID:71371 10 8.663 Arid5b 4930580B11, Mrf2, Mrf2alpha, Mrf2betaAT rich interactive domain 5B (Mrf1 like) GeneID:224647 17 8.663 D17Wsu92e C030010A15 DNA segment, Chr 17, Wayne State University 92, expressed GeneID:331623 10 8.65 AK122525 mKIAA1553 cDNA sequence AK122525 GeneID:67851 10 8.65 1700021F05Rik RIKEN cDNA 1700021F05 gene GeneID:54721 9 8.643 Tyk2 JTK1 2 GeneID:12539 9 8.643 Cdc37 p50 cell division cycle 37 homolog (S. cerevisiae) GeneID:214855 1 8.611 Arid5a MGC36895, Mrf1 AT rich interactive domain 5A (Mrf1 like) GeneID:100647 5 8.554 Upk3b P35, PMS2L14, UpIIIb uroplakin 3B GeneID:69665 5 8.554 2310043J07Rik RIKEN cDNA 2310043J07 gene GeneID:68632 10 8.553 Myct1 Mtmc1 myc target 1 GeneID:14281 12 8.514 Fos c-fos FBJ osteosarcoma oncogene GeneID:229663 3 8.511 Csde1 MGC19174, mKIAA0885, unr cold shock domain containing E1, RNA binding GeneID:18176 3 8.511 Nras N-ras neuroblastoma ras oncogene GeneID:215194 9 8.5 BC021438 MGC29361 cDNA sequence BC021438 GeneID:12581 9 8.5 Cdkn2d INK4d, p19, p19INK4d cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) GeneID:11768 9 8.5 Ap1m2 [m]1B, mu1B adaptor protein complex AP-1, mu 2 subunit GeneID:22195 16 8.482 Ube2l3 UbcM4 ubiquitin-conjugating enzyme E2L 3 GeneID:239731 16 8.482 Gm603 mKIAA1666 gene model 603, (NCBI) GeneID:70397 1 8.438 Tmem70 transmembrane protein 70 GeneID:67923 1 8.438 Tceb1 2610043E24Rik transcription B (SIII), polypeptide 1 GeneID:68581 12 8.423 Tmed10 Tmp21 transmembrane emp24-like trafficking protein 10 (yeast) GeneID:84036 8 8.412 Kcnn1 SK1 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 GeneID:70807 8 8.412 Arrdc2 Ilad1 arrestin domain containing 2 GeneID:212073 12 8.404 4831426I19Rik RIKEN cDNA 4831426I19 gene GeneID:628999 12 8.404 LOC628999 similar to Histone H3.4 (Embryonic) GeneID:11796 9 8.402 Birc3 Api2, Birc2, HIAP2, IAP1, IAP2, MIAP1,baculoviral MIAP2, IAP MIHB, repeat-containing MIHC, RNF49, 3 cIAP-1, cIAP-2, cIAP1, cIAP2 GeneID:12189 11 8.401 Brca1 1 GeneID:21354 17 8.391 Tap1 ABC17, APT1, MTP1, PSF1, RING4,transporter TAP, Y3 1, ATP-binding cassette, sub-family B (MDR/TAP) GeneID:16913 17 8.391 Psmb8 proteosome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) GeneID:16912 17 8.391 Psmb9 proteosome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2) GeneID:19255 18 8.38 Ptpn2 TC-PTP protein tyrosine phosphatase, non-receptor type 2 GeneID:74352 7 8.374 Zfp84 KRAB18, Zfp69 zinc finger protein 84 GeneID:105352 13 8.364 Dusp22 JKAP, JSP1, MKPX, RP23-217J3.1 dual specificity phosphatase 22 GeneID:76295 3 8.348 Atp11b ATPIF, ATPIR, mKIAA0956 ATPase, Class VI, type 11B GeneID:67229 2 8.339 Prpf18 PRP18 pre-mRNA processing factor 18 homolog (yeast) GeneID:66054 18 8.323 Cndp2 CNDP 2 (metallopeptidase M20 family) GeneID:214230 2 8.322 Pak6 4732456M09 (CDKN1A)-activated kinase 6 GeneID:243924 7 8.305 zfp507 mKIAA1084 zinc finger protein 507 GeneID:75859 2 8.299 4930568D16Rik RIKEN cDNA 4930568D16 gene GeneID:74854 2 8.299 4930402F06Rik RIKEN cDNA 4930402F06 gene GeneID:14758 X 8.298 Gpm6b M6B glycoprotein m6b GeneID:76073 19 8.284 Pcgf5 5830406C17Rik polycomb group ring finger 5 GeneID:81018 2 8.278 Zfp313 Zfp228, Znf228 zinc finger protein 313 GeneID:55961 6 8.276 Slc13a1 NaSi-1, Nas1 solute carrier family 13 (sodium/sulphate symporters), member 1 GeneID:217304 11 8.266 Cd300lb Clm7 CD300 antigen like family member B GeneID:104271 8 8.233 Tex15 testis expressed gene 15 GeneID:13609 3 8.233 Edg1 IpB1, S1P1, lpB1 endothelial differentiation sphingolipid G-protein-coupled receptor 1 GeneID:15130 7 8.228 Hbb-b2 hemoglobin, beta adult minor chain GeneID:15129 7 8.228 Hbb-b1 hemoglobin, beta adult major chain GeneID:17187 12 8.215 Max Max protein GeneID:18356 11 8.206 Olfr56 IF7, MOR276-1 olfactory receptor 56 GeneID:432556 11 8.206 LOC432556 similar to hypothetical protein 9930111J21 GeneID:15953 11 8.206 Ifi47 47kDa, IRG-47, Iigp4, Iipg4, Olfr56 interferon gamma inducible protein 47 GeneID:319710 12 8.193 Frmd6 FERM domain containing 6 GeneID:93970 6 8.188 Klra18 Ly49r killer cell lectin-like receptor, subfamily A, member 18 GeneID:27424 6 8.188 Klra16 Klra24, Ly49P, Ly49x killer cell lectin-like receptor, subfamily A, member 16 GeneID:16635 6 8.188 Klra4 Chok, ly49r<129> killer cell lectin-like receptor, subfamily A, member 4 GeneID:56708 19 8.179 Clcf1 CLC cardiotrophin-like cytokine factor 1 GeneID:69745 19 8.179 Pold4 POLDS, p12 polymerase (DNA-directed), delta 4 GeneID:623548 17 8.172 LOC623548 similar to pyridoxal (pyridoxine, vitamin B6) kinase GeneID:67102 16 8.17 D16Ertd472e DNA segment, Chr 16, ERATO Doi 472, expressed GeneID:243655 6 8.166 Klre1 Klre-1, NKG2I killer cell lectin-like receptor family E member 1 GeneID:60361 19 8.15 Ms4a4b Chandra membrane-spanning 4-domains, subfamily A, member 4B GeneID:73656 19 8.15 Ms4a6c membrane-spanning 4-domains, subfamily A, member 6C GeneID:69748 7 8.148 Aldh16a1 MGC19339, mFLJ00209 aldehyde dehydrogenase 16 family, member A1 GeneID:68845 7 8.148 1110061L23Rik RIKEN cDNA 1110061L23 gene GeneID:620371 9 8.129 LOC620371 similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 GeneID:620388 9 8.129 LOC620388 similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 GeneID:620403 9 8.129 LOC620403 hypothetical protein LOC620403 GeneID:11750 14 8.121 Anxa7 Anx7, synexin A7 GeneID:74843 14 8.121 Zmynd17 zinc finger, MYND domain containing 17 GeneID:14709 7 8.108 Gng8 G(y)8 guanine nucleotide binding protein (G protein), gamma 8 subunit GeneID:19222 7 8.108 Ptgir IP I receptor (IP) GeneID:17155 10 8.071 Man1a PCR1 1, alpha GeneID:226517 1 8.025 9430023P16Rik mKIAA0250 RIKEN cDNA 9430023P16 gene GeneID:209334 12 8.007 5830483C08Rik RIKEN cDNA 5830483C08 gene GeneID:67241 12 8.007 Smc6l1 SMC6, mKIAA4103 SMC6 structural maintenance of chromosomes 6-like 1 (yeast) GeneID:53817 17 7.997 Bat1a MGC19235, MGC38799 HLA-B-associated transcript 1A GeneID:14967 17 7.997 H2-D4 histocompatibility 2, D region locus 4 GeneID:14964 17 7.997 H2-D1 H2-D, MHC B7.2 histocompatibility 2, D region locus 1 GeneID:436448 1 7.994 LOC436448 similar to ribosomal protein L18a GeneID:12477 1 7.994 Ctla4 Ly-56 cytotoxic T-lymphocyte-associated protein 4 GeneID:628457 1 7.994 LOC628457 hypothetical protein LOC628457 GeneID:27103 2 7.979 Eif2ak4 2610011M03, GCN2, MGCN2 eukaryotic 2 alpha kinase 4 GeneID:20813 2 7.979 Srp14 14kDa signal recognition particle 14 GeneID:69277 2 7.977 3300002I08Rik RIKEN cDNA 3300002I08 gene GeneID:622232 6 7.971 LOC622232 similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa isoform 1 GeneID:16172 6 7.971 Il17r Cdw217, Il17ra, VDw217 interleukin 17 receptor GeneID:433387 1 7.948 LOC433387 similar to ribosomal protein L21 GeneID:54343 6 7.941 Atf7ip AM activating transcription factor 7 interacting protein GeneID:237339 10 7.941 L3mbtl3 MBT-1 l(3)mbt-like 3 (Drosophila) GeneID:435208 10 7.941 LOC435208 similar to Aldo-keto reductase family 1, member B8 GeneID:234072 8 7.921 Adprhl1 Arh2 ADP-ribosylhydrolase like 1 GeneID:102220 8 7.921 E330037G11Rik RIKEN cDNA E330037G11 gene GeneID:14198 14 7.92 Fhit Fra14A2 fragile histidine triad gene GeneID:225825 18 7.891 Cd226 DNAM-1, DNAM1, Pta1, TLiSA1 CD226 antigen GeneID:12445 17 7.887 Ccnd3 cyclin D3 GeneID:53414 17 7.887 Bysl Bys, Enp1 bystin-like GeneID:235442 9 7.876 Rab8b 5930437D16, D330025I23Rik RAB8B, member RAS oncogene family GeneID:269966 7 7.875 Nup98 4732457F17 nucleoporin 98 GeneID:233575 7 7.875 Frag1 FGF receptor activating protein 1 GeneID:67886 1 7.873 Camsap1l1 mKIAA1078 calmodulin regulated -associated protein 1-like 1 GeneID:103967 1 7.865 Dnm3 C530045C17, mKIAA0820 3 GeneID:474332 1 7.865 Dnm3os Hag2, P/L01 dynamin 3, opposite strand GeneID:19139 X 7.858 Prps1 phosphoribosyl pyrophosphate synthetase 1 GeneID:432800 13 7.856 LOC432800 similar to ets-related protein ER81 GeneID:67759 19 7.855 5033414D02Rik RIKEN cDNA 5033414D02 gene GeneID:60533 19 7.855 Cd274 B7-H1, PD-, Pdcd1l1 CD274 antigen GeneID:232987 7 7.847 BC028440 MGC41256 cDNA sequence BC028440 GeneID:21803 7 7.847 Tgfb1 TGF-beta1 transforming growth factor, beta 1 GeneID:320208 7 7.847 A830041P22Rik RIKEN cDNA A830041P22 gene GeneID:627183 12 7.835 LOC627183 similar to NACHT-, LRR- and PYD-containing protein 2 (PYRIN-containing APAF1-like protein 2) (Nucleotide-binding site protein 1) GeneID:382561 12 7.835 LOC382561 similar to NACHT, leucine rich repeat and PYD containing 5 GeneID:74173 12 7.835 1700012B15Rik RIKEN cDNA 1700012B15 gene GeneID:75547 7 7.818 1700026G02Rik RIKEN cDNA 1700026G02 gene GeneID:140579 2 7.782 Elmo2 CED-12, mKIAA1834 engulfment and cell motility 2, ced-12 homolog (C. elegans) GeneID:224794 17 7.778 Enpp4 MGC29265, mKIAA0879 ectonucleotide pyrophosphatase/phosphodiesterase 4 GeneID:12521 2 7.774 Cd82 C33, Tspan27 CD82 antigen GeneID:11758 1 7.771 Prdx6 1-cysPrx, Aop2-rs3, CP-3, GPx, ORF06,peroxiredoxin Prdx5, Prdx6-rs3, 6 aiPLA2, mKIAA0106 GeneID:66607 19 7.764 Ms4a4d membrane-spanning 4-domains, subfamily A, member 4D GeneID:232201 6 7.758 Arhgap25 Rho GTPase activating protein 25 GeneID:244237 7 7.755 Tnfrsf26 Tnfrsf24 tumor necrosis factor receptor superfamily, member 26 GeneID:79202 7 7.755 Tnfrsf22 SOBa, Tnfrh2, Tnfrsf1al2, mDcTrailr2tumor necrosis factor receptor superfamily, member 22 GeneID:19386 10 7.749 Ranbp2 NUP358 binding protein 2 GeneID:20555 11 7.745 Slfn1 schlafen 1 GeneID:74841 8 7.741 Usp38 mKIAA1891 ubiquitin specific peptidase 38 GeneID:70793 8 7.741 4631402N15Rik RIKEN cDNA 4631402N15 gene GeneID:240921 1 7.727 LOC240921 similar to Ifi204 protein GeneID:12048 2 7.727 Bcl2l1 Bcl-XL, Bcl-Xs, Bcl2l, BclX, bcl-x Bcl2-like 1 GeneID:27405 5 7.725 Abcg3 Abcp2, Mxr2 ATP-binding cassette, sub-family G (WHITE), member 3 GeneID:626471 5 7.725 LOC626471 similar to Aldose reductase (AR) (Aldehyde reductase) GeneID:545790 5 7.725 LOC545790 similar to Protein transport protein SEC61 beta subunit GeneID:433637 3 7.719 LOC433637 hypothetical LOC433637 GeneID:12022 13 7.719 Barx1 BarH-like homeobox 1 GeneID:12775 11 7.717 Ccr7 CD197, Cdw197, EBI1 chemokine (C-C motif) receptor 7 GeneID:18826 14 7.715 Lcp1 L-fimbrin lymphocyte cytosolic protein 1 GeneID:21926 17 7.702 Tnf DIF, TNF-alpha, TNFSF2, TNFalpha,tumor Tnfsf1a, necrosis cytokine factor GeneID:16992 17 7.702 Lta LT, LT-[a], LT-alpha, LT[a], TNF-beta,lymphotoxin TNFSF1, Tnfsf1b, A lymphotoxin GeneID:16994 17 7.702 Ltb LTbeta, Tnfsf3, p33 lymphotoxin B GeneID:71481 3 7.684 Alpk1 LAK alpha-kinase 1 GeneID:211550 3 7.684 T2bp TIFA Traf2 binding protein GeneID:546644 15 7.679 LOC546644 similar to Lymphocyte antigen Ly-6G.1 precursor GeneID:72504 18 7.66 Taf4b 105kDa, TAFII105 TAF4B RNA polymerase II, TATA box binding protein (TBP)-associated factor GeneID:17918 9 7.66 Myo5a MVa, MyoVA, d-120J myosin Va GeneID:218194 13 7.651 Phactr1 phosphatase and actin regulator 1 GeneID:74481 19 7.643 4933430F08Rik RIKEN cDNA 4933430F08 gene GeneID:78016 1 7.634 4930511H11Rik RIKEN cDNA 4930511H11 gene GeneID:69169 1 7.626 Faim3 Toso Fas apoptotic inhibitory molecule 3 GeneID:93672 1 7.626 Il24 FISP, Mda-7, Mda7, St16 interleukin 24 GeneID:66482 13 7.621 Sec5l1 RP23-213G18.3, Sec5 SEC5-like 1 (S. cerevisiae) GeneID:245944 11 7.618 Vps54 Hcc8, Vps54l, mSLP8 vacuolar protein sorting 54 (yeast) GeneID:278097 X 7.604 AW060994 MGC27863 expressed sequence AW060994 GeneID:71703 X 7.604 Armcx3 ALEX3 armadillo repeat containing, X-linked 3 GeneID:105083 13 7.597 Pelo pelota homolog (Drosophila) GeneID:625487 1 7.578 LOC625487 hypothetical protein LOC625487 GeneID:208768 1 7.578 BC031781 MGC30618 cDNA sequence BC031781 GeneID:13033 7 7.577 Ctsd CD, CatD cathepsin D GeneID:26448 12 7.563 Rage MGC46883, MOK, RAGE1 renal tumor antigen GeneID:629622 12 7.563 LOC629622 hypothetical protein LOC629622 GeneID:72805 12 7.563 2810455K09Rik RIKEN cDNA 2810455K09 gene GeneID:432502 10 7.529 LOC432502 similar to ribosomal protein L6 GeneID:237542 10 7.529 Osbpl8 ORP-8 oxysterol binding protein-like 8 GeneID:629132 10 7.529 LOC629132 hypothetical protein LOC629132 GeneID:69296 3 7.526 1700001D09Rik RIKEN cDNA 1700001D09 gene GeneID:11542 3 7.526 Adora3 A3AR, ARA3 A3 receptor GeneID:234542 8 7.514 A330096I21Rik MGC31644, MGC38683, MGC38855,RIKEN Rtbnd cDNA A330096I21 gene GeneID:69724 8 7.514 Rnaseh2a RNASEHI, RNHIA, RNHL ribonuclease H2, large subunit GeneID:19267 7 7.508 Ptpre PTPe, PTPepsilon protein tyrosine phosphatase, receptor type, E GeneID:257630 1 7.498 Il17f IL-17F interleukin 17F GeneID:257632 8 7.496 Card15 ACUG, BLAU, CD, IBD1, Nod2 caspase recruitment domain family, member 15 GeneID:20540 14 7.473 Slc7a7 my+lat1 solute carrier family 7 (cationic amino acid transporter, y+ system), member 7 GeneID:627930 14 7.473 LOC627930 similar to peptidylprolyl isomerase A ( A)-like 4 GeneID:68836 14 7.473 Mrpl52 mitochondrial ribosomal protein L52 GeneID:17387 14 7.473 Mmp14 MT-MMP-1, MT1-MMP matrix metallopeptidase 14 (membrane-inserted) GeneID:258055 7 7.471 Olfr524 MOR103-14P olfactory receptor 524 GeneID:244233 7 7.471 E430002D04Rik B430307C05 RIKEN cDNA E430002D04 gene GeneID:17687 17 7.459 Msh5 G7, Mut5 mutS homolog 5 (E. coli) GeneID:114584 17 7.459 Clic1 Clcp, G6 chloride intracellular channel 1 GeneID:53857 6 7.452 Tuba8 tubulin, alpha 8 GeneID:15944 11 7.441 Irgm Iigp3, Iipg3, LRG-47 immunity-related GTPase family, M GeneID:17191 18 7.42 Mbd2 MBD2a methyl-CpG binding domain protein 2 GeneID:216860 11 7.41 0610025P10Rik KIAA1787, RP23-172M21.17 RIKEN cDNA 0610025P10 gene GeneID:56310 11 7.41 Gps2 G protein pathway suppressor 2 GeneID:276770 11 7.41 Eif5a Eif4d, MGC6725 initiation factor 5A GeneID:18628 4 7.405 Per3 mPer3 period homolog 3 (Drosophila) GeneID:22319 4 7.405 Vamp3 ceb vesicle-associated membrane protein 3 GeneID:20810 4 7.399 Srm SpdSy spermidine synthase GeneID:17175 4 7.399 Masp2 MASP-2, MAp19 mannan-binding lectin serine peptidase 2 GeneID:230908 4 7.399 Tardbp TAR DNA binding protein GeneID:269637 5 7.397 9630008K15Rik RIKEN cDNA 9630008K15 gene GeneID:72016 17 7.386 1600002H07Rik RIKEN cDNA 1600002H07 gene GeneID:12449 17 7.386 Ccnf CycF cyclin F GeneID:16195 13 7.384 Il6st CD130, gp130 interleukin 6 signal transducer GeneID:215900 10 7.374 A630077B13Rik RIKEN cDNA A630077B13 gene GeneID:15950 1 7.372 Ifi203 interferon activated gene 203 GeneID:276950 11 7.367 Slfn8 schlafen 8 GeneID:78388 7 7.366 Mvp LRP, VAULT1 GeneID:67278 7 7.366 2900092E17Rik RIKEN cDNA 2900092E17 gene GeneID:17188 7 7.366 Maz PUR1, Pur-1, SAF-1, SAF-2 MYC-associated zinc finger protein (purine-binding transcription factor) GeneID:13170 7 7.363 Dbp D site albumin promoter binding protein GeneID:56632 7 7.363 Sphk2 sphingosine kinase 2 GeneID:243967 7 7.363 Gm484 gene model 484, (NCBI) GeneID:12348 7 7.363 Car11 carbonic anhydrase 11 GeneID:328833 17 7.357 Treml2 triggering receptor expressed on myeloid cells-like 2 GeneID:76681 7 7.346 Trim12 tripartite motif protein 12 GeneID:625269 7 7.346 LOC625269 hypothetical protein LOC625269 GeneID:66536 4 7.344 Nipsnap3a Nipsnap3b nipsnap homolog 3A (C. elegans) GeneID:11910 1 7.339 Atf3 LRG-21 activating transcription factor 3 GeneID:21961 1 7.328 Tns1 tensin 1 GeneID:228790 2 7.323 Asxl1 mKIAA0978 additional sex combs like 1 (Drosophila) GeneID:12399 4 7.318 Runx3 AML2, Pebp2a3 runt related transcription factor 3 GeneID:72691 19 7.316 2810048G17Rik RIKEN cDNA 2810048G17 gene GeneID:546729 19 7.316 LOC546729 similar to family with sequence similarity 26, member C GeneID:240669 19 7.316 LOC240669 similar to family with sequence similarity 26, member A GeneID:78329 7 7.307 2310010J17Rik RIKEN cDNA 2310010J17 gene GeneID:233489 7 7.307 Picalm CALM, CLTH, MGC19382, MGC36413,phosphatidylinositol MGC36616, PAP180, binding mKIAA41 clathrin14 assembly protein GeneID:277360 2 7.3 BC067047 G630042G04 cDNA sequence BC067047 GeneID:17713 5 7.277 Grpel1 MGC8152, mt-GrpE#1, mt-Grpel1 GrpE-like 1, mitochondrial GeneID:231151 5 7.277 AA474455 expressed sequence AA474455 GeneID:17873 10 7.276 Gadd45b growth arrest and DNA-damage-inducible 45 beta GeneID:21887 9 7.272 Tle3 ESG, Grg3a, Grg3b, mKIAA1547 -like enhancer of split 3, homolog of Drosophila E(spl) GeneID:233280 7 7.237 Nipa1 FSP3 non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) GeneID:21949 4 7.233 Tnfsf8 CD153, CD30LG tumor necrosis factor (ligand) superfamily, member 8 GeneID:71336 5 7.205 Rbks RBSK ribokinase GeneID:107976 5 7.205 Bre brain and reproductive organ-expressed protein GeneID:20448 2 7.192 St6galnac4 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 GeneID:227733 2 7.192 Pip5kl1 phosphatidylinositol-4-phosphate 5-kinase-like 1 GeneID:319245 2 7.192 9430097D07Rik RIKEN cDNA 9430097D07 gene GeneID:14675 19 7.185 Gna14 guanine nucleotide binding protein, alpha 14 GeneID:20971 2 7.177 Sdc4 ryudocan, syndecan-4 syndecan 4 GeneID:50930 17 7.171 Tnfsf14 HVEM-L, HVEML, LIGHT, LTg, Ly113tumor necrosis factor (ligand) superfamily, member 14 GeneID:12266 17 7.171 C3 ASP, Plp complement component 3 GeneID:67725 14 7.169 Nudt13 nudix (nucleoside diphosphate linked moiety X)-type motif 13 GeneID:435565 18 7.169 LOC435565 similar to interferon-inducible GTPase GeneID:60440 18 7.169 Iigp1 Iigp interferon inducible GTPase 1 GeneID:69459 9 7.168 Ubl7 ubiquitin-like 7 ( marrow stromal cell-derived) GeneID:20361 9 7.168 Sema7a CDw108, H-Sema-L, M-Sema-L sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A GeneID:14284 5 7.164 Fosl2 Fra-2 fos-like antigen 2 GeneID:20194 3 7.163 S100a10 42C, CAL12, CLP11, p10, p11 S100 calcium binding protein A10 (calpactin) GeneID:17289 2 7.143 Mertk Eyk, Nyk c-mer proto-oncogene tyrosine kinase GeneID:17222 2 7.143 Anapc1 Apc1, tsg24 anaphase promoting complex subunit 1 GeneID:13030 14 7.143 Ctsb cathepsin B GeneID:241633 2 7.142 Atp8b4 A530043E15, Im ATPase, class I, type 8B, member 4 GeneID:628329 19 7.129 LOC628329 similar to high mobility group nucleosomal binding domain 1 (predicted) GeneID:14102 19 7.129 Fas APO-1, APT1, CD95, TNFR6 Fas (TNF receptor superfamily member) GeneID:74450 2 7.108 Pank2 pantothenate kinase 2 (Hallervorden-Spatz syndrome) GeneID:69542 17 7.084 2300002M23Rik RIKEN cDNA 2300002M23 gene GeneID:225348 18 7.074 Wdr36 MGC38421, Ta-wdrp WD repeat domain 36 GeneID:102644 9 7.039 D9Ucla1 DNA segment, Chr 9, University of California at Los Angeles 1 GeneID:235302 9 7.039 D630033O11Rik RIKEN cDNA D630033O11 gene GeneID:71865 10 7.03 Fbxo30 F-box protein 30 GeneID:109889 7 7.011 Zfp98 Mzf-2, Mzf1, Mzf2, Znf42 zinc finger protein 98 GeneID:67287 9 7.002 Parp6 poly (ADP-ribose) polymerase family, member 6 GeneID:18746 9 7.002 Pkm2 pyruvate kinase, muscle GeneID:623121 1 6.996 LOC623121 similar to Interferon-activatable protein 203 (Ifi-203) (Interferon-inducible protein p203) GeneID:627018 13 6.981 LOC627018 hypothetical protein LOC627018 GeneID:78521 13 6.981 B230219D22Rik RIKEN cDNA B230219D22 gene GeneID:12175 9 6.968 Bnip2 BNIP2beta, Nip21 BCL2/adenovirus E1B 19kDa-interacting protein 1, NIP2 GeneID:624164 9 6.968 LOC624164 hypothetical protein LOC624164 GeneID:235459 9 6.968 Gtf2a2 12kDa, TfIIg general transcription factor II A, 2 GeneID:68146 16 6.962 Arl2l1 ADP-ribosylation factor-like 2-like 1 GeneID:19128 16 6.962 Pros1 protein S (alpha) GeneID:54324 6 6.955 Arhgef5 Tim1 Rho guanine nucleotide exchange factor (GEF) 5 GeneID:622672 14 6.953 LOC622672 similar to esterase D/formylglutathione GeneID:69740 3 6.944 Dph5 DPH5 homolog (S. cerevisiae) GeneID:625609 13 6.943 LOC625609 hypothetical protein LOC625609 GeneID:223693 15 6.942 4732495E13Rik 2610507A11 RIKEN cDNA 4732495E13 gene GeneID:14270 1 6.93 Srgap2 FBP2, srGAP3 SLIT-ROBO Rho GTPase GeneID:56489 1 6.93 Ikbke IKK-i, IKKepsilon, Ikki inhibitor of kappaB kinase epsilon GeneID:23958 9 6.924 Nr2e3 PNR, RNR subfamily 2, group E, member 3 GeneID:231932 6 6.918 Gimap7 IAN7, MGC41480 GTPase, IMAP family member 7 GeneID:16438 6 6.918 Itpr1 IP3R1, InsP3R, P400 inositol 1,4,5-triphosphate receptor 1 GeneID:67045 17 6.901 Riok2 RIO kinase 2 (yeast) GeneID:66643 17 6.901 Lix1 limb expression 1 homolog (chicken) GeneID:226539 1 6.893 Dars2 aspartyl-tRNA synthetase 2 (mitochondrial) GeneID:70454 1 6.893 2610300B10Rik RIKEN cDNA 2610300B10 gene GeneID:216393 10 6.88 D930020B18Rik RIKEN cDNA D930020B18 gene GeneID:71778 5 6.865 Klhl5 kelch-like 5 (Drosophila) GeneID:78581 15 6.861 D530033C11Rik RIKEN cDNA D530033C11 gene GeneID:229504 3 6.852 Isg20l2 4930429N24 interferon stimulated exonuclease gene 20-like 2 GeneID:12904 3 6.852 Crabp2 CrabpII cellular retinoic acid binding protein II GeneID:69368 1 6.841 Wdfy1 FENS-1, Jr1, WDF1, ZFYVE17, mKIAA1435WD repeat and FYVE domain containing 1 GeneID:69163 1 6.841 Mrpl44 mitochondrial ribosomal protein L44 GeneID:17454 3 6.839 Mov10 Moloney leukemia virus 10 GeneID:12340 3 6.839 Capza1 CAPZ, CAZ1 capping protein (actin filament) muscle Z-line, alpha 1 GeneID:67895 10 6.839 Ppa1 pyrophosphatase (inorganic) 1 GeneID:27054 2 6.836 Sec23b SEC23B (S. cerevisiae) GeneID:228715 2 6.836 Gm561 gene model 561, (NCBI) GeneID:66044 2 6.836 Hars2 histidyl tRNA synthetase 2 GeneID:11783 10 6.835 Apaf1 Apaf-1, mKIAA0413 apoptotic peptidase activating factor 1 GeneID:67454 10 6.835 1200009F10Rik Ikip RIKEN cDNA 1200009F10 gene GeneID:21984 13 6.835 Tpbpa 4311 trophoblast specific protein alpha GeneID:116913 13 6.835 Tpbpb trophoblast specific protein beta GeneID:50493 10 6.832 Txnrd1 TR, TR1, Tgr, TrxR1 reductase 1 GeneID:66341 10 6.832 1700027M21Rik RIKEN cDNA 1700027M21 gene GeneID:21652 17 6.823 Phf1 PHF2, mPcl1 PHD finger protein 1 GeneID:67675 17 6.823 0610039D01Rik Cuta RIKEN cDNA 0610039D01 gene GeneID:240057 17 6.823 Syngap1 Syngap synaptic Ras GTPase activating protein 1 homolog (rat) GeneID:16580 17 6.823 Kifc1 HSET, KNSL2 kinesin family member C1 GeneID:59027 12 6.796 Pbef1 Nampt, Pbef pre-B-cell colony-enhancing factor 1 GeneID:14546 12 6.796 Gdap10 ganglioside-induced differentiation-associated-protein 10 GeneID:16975 4 6.79 Lrp8 apoER2 low density lipoprotein receptor-related protein 8, apolipoprotein e receptor GeneID:17132 8 6.788 Maf c- avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog GeneID:58208 12 6.788 Bcl11b Ctip2, Rit1 B-cell leukemia/lymphoma 11B GeneID:319630 12 6.788 A130014H13Rik RIKEN cDNA A130014H13 gene GeneID:381695 5 6.787 BC037393 MGC25830 cDNA sequence BC037393 GeneID:74377 17 6.783 Hsf2bp heat shock transcription factor 2 binding protein GeneID:72462 17 6.783 2600005C20Rik D030064A17, Kiaa0179, mKIAA0179RIKEN cDNA 2600005C20 gene GeneID:100072 4 6.77 Camta1 mKIAA0833 calmodulin binding transcription activator 1 GeneID:224661 17 6.762 Slc26a8 MGC38861 solute carrier family 26, member 8 GeneID:26416 17 6.762 Mapk14 CSBP2, Mxi2, PRKM14, PRKM15, p38,mitogen p38-alpha, activated p38MAPK protein kinase 14 GeneID:231713 5 6.76 C330023M02Rik A630046C11 RIKEN cDNA C330023M02 gene GeneID:11540 10 6.756 Adora2a A2AAR, A2aR GeneID:19347 7 6.749 Rab6ip1 ORF37, mKIAA1091 Rab6 interacting protein 1 GeneID:71704 14 6.737 Arhgef3 Rho guanine nucleotide exchange factor (GEF) 3 GeneID:14375 15 6.729 Xrcc6 70kDa, Ku70 X-ray repair complementing defective repair in Chinese hamster cells 6 GeneID:20826 15 6.729 Nhp2l1 FA-1 NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) GeneID:15900 8 6.726 Irf8 ICSBP, IRF-8 interferon regulatory factor 8 GeneID:67711 7 6.723 Nsmce1 non-SMC element 1 homolog (S. cerevisiae) GeneID:71198 2 6.722 Otud1 OTU domain containing 1 GeneID:210293 1 6.72 Dock10 Jr4, Jr5, ZIZ3, Zizimin3, mKIAA0694dedicator of cytokinesis 10 GeneID:14607 11 6.718 Gip gastric inhibitory polypeptide GeneID:27681 11 6.718 Snf8 MGC7335 SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) GeneID:19219 15 6.71 Ptger4 EP4 prostaglandin E receptor 4 (subtype EP4) GeneID:16890 7 6.703 Lipe HSL lipase, hormone sensitive GeneID:71472 9 6.698 Usp19 Zmynd9 ubiquitin specific peptidase 19 GeneID:97541 9 6.698 Qars GLNRS glutaminyl-tRNA synthetase GeneID:69232 9 6.698 2610028H07Rik RIKEN cDNA 2610028H07 gene GeneID:269587 4 6.692 Epb4.1 4.1R, mKIAA4056 erythrocyte protein band 4.1 GeneID:18034 19 6.691 Nfkb2 NF-kappaB2, lyt, p49, p49/p100, p50B,nuclear p52 factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100 GeneID:73728 19 6.691 Psd A930015K15, AI426354, Efa6, mKIAA201pleckstrin1 and Sec7 domain containing GeneID:244713 9 6.667 Zfp75 4932416G03, KRAB9, Zfp67, mszf40zinc finger protein 75 GeneID:242860 5 6.655 Rsbn1l round spermatid basic protein 1-like GeneID:19186 14 6.651 Psme1 PA28a proteasome (prosome, macropain) 28 subunit, alpha GeneID:85308 14 6.651 1500005A01Rik Cgi112 RIKEN cDNA 1500005A01 gene GeneID:19188 14 6.651 Psme2 PA28b proteasome (prosome, macropain) 28 subunit, beta GeneID:19191 14 6.651 Psme2b PA28b-p, PA28b2, Psme2-like (prosome, macropain) 28 subunit, beta, b GeneID:234733 8 6.648 Ddx19b DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b GeneID:234734 8 6.648 Aars MGC37368 alanyl-tRNA synthetase GeneID:110826 7 6.642 Etfb electron transferring flavoprotein, beta polypeptide GeneID:233187 7 6.642 Lim2 19kDa, 4833403J20, MP19 lens intrinsic membrane protein 2 GeneID:72310 7 6.642 Nkg7 group 7 sequence GeneID:74276 7 6.642 1700071E18Rik RIKEN cDNA 1700071E18 gene GeneID:360213 3 6.605 Trim46 tripartite motif protein 46 GeneID:21827 3 6.605 Thbs3 thrombospondin 3 GeneID:17829 3 6.605 Muc1 CD227, EMA mucin 1, transmembrane GeneID:66940 9 6.603 Scotin scotin gene GeneID:235610 9 6.603 6620401K05Rik 5930438A12 RIKEN cDNA 6620401K05 gene GeneID:22040 9 6.603 Trex1 1661 three prime repair exonuclease 1 GeneID:223453 15 6.599 Dap MGC19154, MGC6998 death-associated protein GeneID:14191 4 6.599 Fgr Gardner-Rasheed feline sarcoma viral (Fgr) oncogene homolog GeneID:381605 4 6.592 A630005A06Rik RIKEN cDNA A630005A06 gene GeneID:105559 14 6.589 Mbnl2 MBLL, mKIAA4072 muscleblind-like 2 GeneID:620177 6 6.579 LOC620177 hypothetical protein LOC620177 GeneID:620192 6 6.579 LOC620192 similar to ribosomal protein L38 GeneID:231821 5 6.567 Centa1 GC1L, p42IP4 centaurin, alpha 1 GeneID:192136 13 6.566 AF397014 cDNA sequence AF397014 GeneID:66308 13 6.566 2810021B07Rik RIKEN cDNA 2810021B07 gene GeneID:56353 6 6.562 Rybp MGC62213, YEAF1 RING1 and YY1 binding protein GeneID:13034 1 6.558 Ctse CE, CatE cathepsin E GeneID:22294 X 6.551 Uxt ubiquitously expressed transcript GeneID:320742 X 6.551 A230072C01Rik RIKEN cDNA A230072C01 gene GeneID:622905 7 6.532 LOC622905 similar to nuclear mitotic apparatus protein 1 GeneID:12609 16 6.515 Cebpd CCAAT/enhancer binding protein (C/EBP), delta GeneID:224008 16 6.515 2310008H04Rik MGC30630 RIKEN cDNA 2310008H04 gene GeneID:12192 12 6.515 Zfp36l1 Berg36, Brf1, ERF1, TIS11b, cMG1 zinc finger protein 36, C3H type-like 1 GeneID:21915 1 6.507 Dtymk CDC8, TMPK, TYPK, mtmk deoxythymidylate kinase GeneID:66262 1 6.507 Ing5 1700001C14Rik inhibitor of growth family, member 5 GeneID:72244 7 6.505 1600014C10Rik RIKEN cDNA 1600014C10 gene GeneID:26932 12 6.504 Ppp2r5e protein phosphatase 2, regulatory subunit B (B56), epsilon isoform GeneID:16822 11 6.5 Lcp2 SLP-76 lymphocyte cytosolic protein 2 GeneID:69787 15 6.498 Anxa13 annexin A13 GeneID:236451 14 6.494 LOC236451 similar to PHD finger protein 11 GeneID:331476 X 6.49 LOC331476 similar to sentrin 15 GeneID:69713 X 6.49 Pin4 EPVH, Par14 protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin) GeneID:112419 19 6.49 2010002M12Rik RIKEN cDNA 2010002M12 gene GeneID:16768 6 6.474 Lag3 CD223, LAG-3 lymphocyte-activation gene 3 GeneID:69202 6 6.474 2610009E16Rik Ptms RIKEN cDNA 2610009E16 gene GeneID:442825 6 6.474 A230083G16Rik RIKEN cDNA A230083G16 gene GeneID:208583 9 6.471 Nek11 NIMA (never in mitosis gene a)-related expressed kinase 11 GeneID:66595 9 6.471 1100001A21Rik RIKEN cDNA 1100001A21 gene GeneID:50760 7 6.47 Fbxo17 FBXO26, Fbg4, Fbx17 F-box protein 17 GeneID:109929 12 6.459 Zbtb25 zinc finger and BTB domain containing 25 GeneID:268564 12 6.459 Zbtb1 9430077A12, Bsg2 zinc finger and BTB domain containing 1 GeneID:30927 8 6.445 Snai3 Smuc snail homolog 3 (Drosophila) GeneID:68718 8 6.445 Rnf166 Zfp313l ring finger protein 166 GeneID:13654 10 6.439 Egr2 Krox20, NGF1-B, Zfp-6 early growth response 2 GeneID:211488 10 6.439 Gm237 MGC73511 gene model 237, (NCBI) GeneID:67283 11 6.422 Slc25a19 DNC, MUP1 solute carrier family 25 (mitochondrial deoxynucleotide carrier), member 19 GeneID:225594 18 6.417 LOC225594 similar to CDNA sequence BC023105 GeneID:629284 18 6.417 LOC629284 hypothetical protein LOC629284 GeneID:66141 7 6.396 Ifitm3 Cd225, Cdw217, IP15, mil-1 interferon induced transmembrane protein 3 GeneID:213002 7 6.396 Ifitm6 A330075D07, Fgls5, fragilis5 interferon induced transmembrane protein 6 GeneID:19046 5 6.391 Ppp1cb 1200010B19 protein phosphatase 1, catalytic subunit, beta isoform GeneID:74326 4 6.387 Hnrpr hnRNPR heterogeneous nuclear ribonucleoprotein R GeneID:625009 4 6.387 LOC625009 hypothetical protein LOC625009 GeneID:14872 10 6.377 Gstt2 Yrs, mGSTT2 S-transferase, theta 2 GeneID:75886 10 6.377 4930583C14Rik RIKEN cDNA 4930583C14 gene GeneID:380840 13 6.377 BC034664 MGC38622 cDNA sequence BC034664 GeneID:69955 13 6.377 Fars2 phenylalanine-tRNA synthetase 2 (mitochondrial) GeneID:84653 11 6.362 Hes7 hairy and enhancer of split 7 (Drosophila) GeneID:23801 11 6.362 Aloxe3 e-LOX-3 arachidonate lipoxygenase 3 GeneID:18626 11 6.362 Per1 Per, m-rigui, mPer1 period homolog 1 (Drosophila) GeneID:213027 8 6.346 B130050I23Rik EVI5L, MGC29047 RIKEN cDNA B130050I23 gene GeneID:628324 3 6.342 LOC628324 similar to S100 calcium-binding protein A2 (S-100L protein) (CAN19) GeneID:64051 3 6.34 Sv2a mKIAA0736 synaptic vesicle glycoprotein 2 a GeneID:69168 3 6.34 Bola1 bolA-like 1 (E. coli) GeneID:218210 13 6.336 Nup153 GeneID:19331 6 6.333 Rab19 RAB19, member RAS oncogene family GeneID:53860 11 6.329 9-Sep MSF1, PNUTL4, Sint1 septin 9 GeneID:239273 14 6.328 Abcc4 MOAT-B, MOATB, MRP4 ATP-binding cassette, sub-family C (CFTR/MRP), member 4 GeneID:240087 17 6.322 Mdc1 6820401C03, NFBD1, mKIAA0170 mediator of DNA damage checkpoint 1 GeneID:106582 17 6.322 Nrm nurim ( membrane protein) GeneID:76448 17 6.322 2310014H01Rik RIKEN cDNA 2310014H01 gene GeneID:19345 11 6.32 Rab5c Rabl RAB5C, member RAS oncogene family GeneID:380728 11 6.32 LOC380728 similar to potassium voltage-gated channel, subfamily H, member 4 GeneID:79263 17 6.315 Trim39 RBCC-B30.2, mKIAA4179, tfp tripartite motif protein 39 GeneID:625892 17 6.315 LOC625892 hypothetical protein LOC625892 GeneID:333715 17 6.315 H2-M10.2 4.7H histocompatibility 2, M region locus 10.2 GeneID:27357 3 6.291 Gyg1 1 GeneID:52715 11 6.288 D11Ertd707e DNA segment, Chr 11, ERATO Doi 707, expressed GeneID:629955 11 6.288 LOC629955 similar to Dbf4-related factor 1 isoform 1 GeneID:18613 11 6.285 Pecam1 PECAM-1, Pecam platelet/endothelial 1 GeneID:66614 3 6.285 Gpatc4 G patch domain containing 4 GeneID:237859 11 6.279 AI851076 8030451K07, RP23-100P23.2 expressed sequence AI851076 GeneID:433501 2 6.273 LOC433501 similar to RIKEN cDNA 5330426L24 GeneID:28193 10 6.265 D10Ucla1 Reep3 DNA segment, Chr 10, University of California at Los Angeles 1 GeneID:66611 X 6.255 Ribc1 RIB43A domain with coiled-coils 1 GeneID:24061 X 6.255 Smc1l1 SB1.8, SMCB, Smc1, Smc1alpha, mKIAA0178SMC (structural maintenance of chromosomes 1)-like 1 (S. cerevisiae) GeneID:20359 17 6.241 Sema6b Sema, VIb, semaZ sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B GeneID:66464 4 6.241 Taf12 20kDa, Taf2J TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor GeneID:14425 2 6.231 Galnt3 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 GeneID:620893 2 6.231 LOC620893 hypothetical protein LOC620893 GeneID:17082 1 6.227 Il1rl1 DER4, Fit-1, ST2L, T1, T1/ST2 interleukin 1 receptor-like 1 GeneID:16182 1 6.227 Il18r1 Il18ralpha interleukin 18 receptor 1 GeneID:17133 15 6.221 Maff v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian) GeneID:239790 16 6.219 Ostn Ostc osteocrin GeneID:224065 16 6.219 Uts2d U2B urotensin 2 domain containing GeneID:208263 1 6.218 Tor1aip1 MGC6357 interacting protein 1 GeneID:64164 1 6.218 Ifrg15 15kDa interferon alpha responsive gene GeneID:16641 6 6.218 Klrc1 CD159a, NKG2A, NKG2B killer cell lectin-like receptor subfamily C, member 1 GeneID:384497 6 6.218 LOC384497 similar to RIKEN cDNA E030024M05 GeneID:19401 11 6.216 Rara Nr1b1, RAR, RARalpha1 , alpha GeneID:234076 8 6.214 Tmco3 transmembrane and coiled-coil domains 3 GeneID:12895 15 6.212 Cpt1b M-CPTI carnitine palmitoyltransferase 1b, muscle GeneID:12651 15 6.212 Chkb CK/EK, CK/EK-beta choline kinase beta GeneID:319362 15 6.212 C730034F03Rik RIKEN cDNA C730034F03 gene GeneID:54325 4 6.209 Elovl1 Ssc1 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1 GeneID:107995 4 6.209 Cdc20 p55CDC cell division cycle 20 homolog (S. cerevisiae) GeneID:14939 14 6.207 Gzmb CCP-1/C11, CCP1 granzyme B GeneID:628418 14 6.207 LOC628418 similar to High mobility group protein 1 (HMG-1) (High mobility group protein B1) (Amphoterin) (Heparin-binding protein p30) GeneID:278279 10 6.206 8430438D04Rik RIKEN cDNA 8430438D04 gene GeneID:71722 7 6.205 Cic mKIAA0306 capicua homolog (Drosophila) GeneID:110052 13 6.204 Dek DEK oncogene (DNA binding) GeneID:22720 11 6.198 Zfp62 zinc finger protein 62 GeneID:14794 6 6.19 Spsb2 C9, SSB2 splA/ domain and SOCS box containing 2 GeneID:21991 6 6.19 Tpi1 Tpi triosephosphate isomerase 1 GeneID:22225 6 6.19 Usp5 ISOT, ISOT-1 ubiquitin specific peptidase 5 (isopeptidase T) GeneID:14251 17 6.188 Flot1 flotillin 1 GeneID:15937 17 6.188 Ier3 IEX-1, cI-3, gly96 immediate early response 3 GeneID:230917 4 6.184 D4Ertd429e 9430091K04 DNA segment, Chr 4, ERATO Doi 429, expressed GeneID:70556 4 6.184 5730438N18Rik MGC4399 RIKEN cDNA 5730438N18 gene GeneID:66687 10 6.183 Tbc1d15 TBC1 domain family, member 15 GeneID:20842 9 6.179 Stag1 SA-1 stromal antigen 1 GeneID:22781 10 6.177 Zfpn1a4 Eos, Znfn1a4 zinc finger protein, subfamily 1A, 4 (Eos) GeneID:15976 16 6.169 Ifnar2 Ifnar-2 interferon (alpha and beta) receptor 2 GeneID:76074 5 6.161 5830443L24Rik RIKEN cDNA 5830443L24 gene GeneID:236573 5 6.161 E430029F06 hypothetical protein E430029F06 GeneID:626509 5 6.161 LOC626509 similar to membrane-associated ring finger (C3HC4) 2 GeneID:17472 5 6.161 Mpa2 Gbp4, Mag-2, mKIAA4245 activation 2 GeneID:73287 10 6.161 1700040L02Rik RIKEN cDNA 1700040L02 gene GeneID:15441 4 6.148 Hp1bp3 Hp1bp74, MGC28927 heterochromatin protein 1, binding protein 3 GeneID:230861 4 6.148 Eif4g3 4833436O05, eIF4GII eukaryotic translation initiation factor 4 gamma, 3 GeneID:76551 10 6.148 2810012H18Rik RIKEN cDNA 2810012H18 gene GeneID:225280 18 6.125 D030070L09Rik RIKEN cDNA D030070L09 gene GeneID:625515 18 6.125 LOC625515 hypothetical protein LOC625515 GeneID:56275 19 6.125 Rbm14 Sytip, p16, p16K RNA binding motif protein 14 GeneID:19653 19 6.125 Rbm4 4921506I22Rik, Mlark, lark RNA binding motif protein 4 GeneID:109658 4 6.124 Txlna taxilin alpha GeneID:16650 4 6.124 Kpna6 IPOA7, Kpna5, NPI-2 karyopherin (importin) alpha 6 GeneID:219131 14 6.104 Phf11 PHD finger protein 11 GeneID:11891 9 6.096 Rab27a RAB27A, member RAS oncogene family GeneID:14385 9 6.091 Slc37a4 G6PT, GSD-1b, mG6PT solute carrier family 37 (glycerol-6-phosphate transporter), member 4 GeneID:60409 9 6.091 Trappc4 HSPC172, PTD009, Sbd, Sdcbp2, TRS23trafficking protein particle complex 4 GeneID:75617 9 6.091 Rps25 ribosomal protein S25 GeneID:382073 9 6.091 D630044F24Rik RIKEN cDNA D630044F24 gene GeneID:230822 4 6.088 A330049M08Rik RIKEN cDNA A330049M08 gene GeneID:53902 4 6.088 Dscr1l2 Down syndrome critical region gene 1-like 2 GeneID:263876 2 6.086 Spata2 MGC38980, mKIAA0757 spermatogenesis associated 2 GeneID:58249 19 6.078 Fibp fibroblast growth factor (acidic) intracellular binding protein GeneID:13041 19 6.078 Ctsw lymphopain cathepsin W GeneID:14283 19 6.078 Fosl1 Fra1, fra-1 fos-like antigen 1 GeneID:240514 19 6.078 AI842788 MGC67311 expressed sequence AI842788 GeneID:20351 3 6.072 Sema4a SemB sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A GeneID:67702 1 6.072 Rnf149 Greul4 ring finger protein 149 GeneID:67182 4 6.07 Pdzk1ip1 PDZK1 interacting protein 1 GeneID:319172 13 6.07 Hist1h2ab H2A.1, H2a-53 histone 1, H2ab GeneID:319150 13 6.07 Hist1h3b H3-53 histone 1, H3b GeneID:326620 13 6.07 Hist1h4b histone 1, H4b GeneID:326619 13 6.07 Hist1h4a histone 1, H4a GeneID:360198 13 6.07 Hist1h3a histone 1, H3a GeneID:80838 13 6.07 Hist1h1a H1.1, H1a, H1var3 histone 1, H1a GeneID:72649 6 6.07 2700094F01Rik RIKEN cDNA 2700094F01 gene GeneID:75647 6 6.07 1700025E21Rik RIKEN cDNA 1700025E21 gene GeneID:232566 6 6.069 C730024G19Rik A230083D09 RIKEN cDNA C730024G19 gene GeneID:109552 5 6.061 Sri sorcin GeneID:433840 5 6.061 LOC433840 similar to ribosomal protein L27 GeneID:14012 9 6.054 Eva1 Eva, Mpzl2 epithelial V-like antigen 1 GeneID:319742 9 6.054 A530065I17Rik RIKEN cDNA A530065I17 gene GeneID:20768 7 6.043 Sephs2 selenophosphate synthetase 2 GeneID:628838 2 6.037 LOC628838 hypothetical protein LOC628838 GeneID:66892 6 6.035 Eif4e3 eIF4E-3 eukaryotic translation initiation factor 4E member 3 GeneID:230257 4 6.034 Rod1 MGC11742 ROD1 regulator of differentiation 1 (S. pombe) GeneID:620625 4 6.034 LOC620625 hypothetical protein LOC620625 GeneID:72290 11 6.022 Lsm11 RP23-298M7.5 U7 snRNP-specific Sm-like protein LSM11 GeneID:66628 11 6.022 5730409G07Rik RP23-298M7.6 RIKEN cDNA 5730409G07 gene GeneID:22388 5 6.022 Wdr1 WD repeat domain 1 GeneID:67181 11 6.02 Dullard Dullard homolog (Xenopus laevis) GeneID:56486 11 6.02 Gabarap gamma-aminobutyric acid receptor associated protein GeneID:78246 11 6.02 Phf23 JUNE-1, RP23-172M21.13 PHD finger protein 23 GeneID:13543 11 6.02 Dvl2 dishevelled 2, dsh homolog (Drosophila) GeneID:72590 7 6.019 2700017M01Rik Pme1 RIKEN cDNA 2700017M01 gene GeneID:277939 7 6.019 AU020772 expressed sequence AU020772 GeneID:99696 3 6.007 E430012K20Rik mKIAA1223, mKIAA3019 RIKEN cDNA E430012K20 gene GeneID:16154 9 5.998 Il10ra CDw210, mIL-10R receptor, alpha GeneID:14201 4 5.982 Fhl3 four and a half LIM domains 3 GeneID:67776 9 5.965 Loh11cr2a BCSC-1, E130119J22 loss of heterozygosity, 11, chromosomal region 2, gene A homolog (human) GeneID:70122 4 5.958 Mllt3 Af9 myeloid/lymphoid or mixed lineage-leukemia translocation to 3 homolog (Drosophila) GeneID:13478 9 5.95 Dpagt1 dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) GeneID:15270 9 5.95 H2afx H2A.X H2A histone family, member X GeneID:15288 9 5.95 Hmbs PBGD hydroxymethylbilane synthase GeneID:12045 9 5.949 Bcl2a1b A1-b B-cell leukemia/lymphoma 2 related protein A1b GeneID:11629 17 5.945 Aif1 AIF-1, G1, Iba1 allograft inflammatory factor 1 GeneID:53761 17 5.945 Bat2 G2, Wbp12, mKIAA4178 HLA-B associated transcript 2 GeneID:56336 2 5.939 B4galt5 UDP-Gal:betaGlcNAc beta 1,4-, polypeptide 5 GeneID:66206 19 5.934 1110059E24Rik RIKEN cDNA 1110059E24 gene GeneID:226016 19 5.934 5730446C15Rik Cgi67, MGC40949 RIKEN cDNA 5730446C15 gene GeneID:271457 17 5.931 Rab5a RAB5A, member RAS oncogene family GeneID:66497 16 5.92 2610528E23Rik RIKEN cDNA 2610528E23 gene GeneID:16373 8 5.917 Irx3 Iroquois related homeobox 3 (Drosophila) GeneID:319388 8 5.917 D230002A01Rik RIKEN cDNA D230002A01 gene GeneID:26410 18 5.909 Map3k8 Cot/Tpl2, Est, Estf, Tpl-2, c-COT mitogen activated protein kinase kinase kinase 8 GeneID:74121 2 5.907 Acoxl acyl-Coenzyme A oxidase-like GeneID:12125 2 5.907 Bcl2l11 Bod BCL2-like 11 (apoptosis facilitator) GeneID:76183 9 5.906 Brunol6 CELF6 bruno-like 6, RNA binding protein (Drosophila) GeneID:217166 11 5.901 Nr1d1 nuclear receptor subfamily 1, group D, member 1 GeneID:21833 11 5.901 Thra Nr1a1, T3R[a], T3Ralpha alpha GeneID:629554 9 5.896 LOC629554 hypothetical protein LOC629554 GeneID:66660 9 5.888 5730555F13Rik Met RIKEN cDNA 5730555F13 gene GeneID:109284 10 5.887 C030046I01Rik RIKEN cDNA C030046I01 gene GeneID:114229 10 5.887 Gpr54 G protein-coupled receptor 54 GeneID:13496 10 5.887 Arid3a Bright AT rich interactive domain 3A (Bright like) GeneID:12522 13 5.878 Cd83 CD83 antigen GeneID:22352 2 5.868 Vim GeneID:108907 2 5.85 Nusap1 ANKT, BM037, LNP, NuSAP, Q0310,nucleolar SAPL and spindle associated protein 1 GeneID:66253 10 5.846 Aig1 CGI-103 androgen-induced 1 GeneID:72084 16 5.839 Pigx phosphatidylinositol glycan, class X GeneID:224105 16 5.839 Pak2 PAK-2, mKIAA4182 p21 (CDKN1A)-activated kinase 2 GeneID:627233 16 5.839 LOC627233 similar to H2A histone family, member Z GeneID:19664 5 5.838 Rbpsuh CBF1, Igkjrb, RBP-J, RBPjk recombining binding protein suppressor of hairless (Drosophila) GeneID:69861 19 5.837 2010003K11Rik RIKEN cDNA 2010003K11 gene GeneID:626257 14 5.826 LOC626257 similar to zinc finger protein 277 isoform 1 GeneID:554327 14 5.826 LOC554327 hypothetical protein LOC554327 GeneID:70381 5 5.819 2210010N04Rik mKIAA1358 RIKEN cDNA 2210010N04 gene GeneID:68479 15 5.817 Phf5a PHD finger protein 5A GeneID:11429 15 5.817 Aco2 aconitase 2, mitochondrial GeneID:20821 7 5.81 Trim21 Ro52 tripartite motif protein 21 GeneID:384699 7 5.81 LOC384699 similar to Non-catalytic region of tyrosine kinase adaptor protein 1 GeneID:74597 17 5.798 4833418N02Rik RIKEN cDNA 4833418N02 gene GeneID:225049 17 5.798 Ttc7 tetratricopeptide repeat domain 7 GeneID:27049 3 5.798 Etv3 METS ets variant gene 3 GeneID:103573 11 5.794 Xpo1 Crm1 exportin 1, CRM1 homolog (yeast) GeneID:215413 1 5.793 A230074B11Rik RIKEN cDNA A230074B11 gene GeneID:13713 10 5.791 Elk3 Erp, Etrp, Net, Sap-2 ELK3, member of ETS oncogene family GeneID:16012 15 5.787 Igfbp6 IGFBP-6 -like growth factor binding protein 6 GeneID:223920 15 5.787 Soat2 ACAT2, MGC36039 sterol O-acyltransferase 2 GeneID:23959 9 5.783 Nt5e CD73, NT, eNT 5' nucleotidase, ecto GeneID:319178 13 5.783 Hist1h2bb H2b-143 histone 1, H2bb GeneID:319148 13 5.783 Hist1h3c H3-143 histone1, H3c GeneID:50708 13 5.783 Hist1h1c H1.2, H1c, H1var1, His1a histone 1, H1c GeneID:227929 2 5.775 Pscdbp CYTIP, Cbp, tamalin pleckstrin homology, Sec7 and coiled-coil domains, binding protein GeneID:19142 3 5.774 Prss12 Bssp-3 protease, serine, 12 neurotrypsin (motopsin) GeneID:11845 12 5.765 Arf6 ADP-ribosylation factor 6 GeneID:67980 5 5.762 Gnpda2 GNPDA, Gnp2, Sb52, mKIAA4008 glucosamine-6-phosphate deaminase 2 GeneID:12874 11 5.751 Cpd D GeneID:233902 7 5.749 Fbxl19 6030430O11, Fbl19 F-box and leucine-rich repeat protein 19 GeneID:69774 19 5.746 Ms4a6b membrane-spanning 4-domains, subfamily A, member 6B GeneID:381214 19 5.746 LOC381214 similar to membrane-spanning 4-domains, subfamily A, member 4C GeneID:56323 4 5.743 Dnajb5 Hsc40, Hsp40-3 DnaJ (Hsp40) homolog, subfamily B, member 5 GeneID:436348 16 5.738 LOC436348 similar to ribosomal protein L35a GeneID:621868 11 5.737 LOC621868 similar to HYPOTHETICAL PROTEIN ORF-1137 GeneID:12912 1 5.733 Creb1 3526402H21Rik cAMP responsive element binding protein 1 GeneID:67099 1 5.733 2310038H17Rik RIKEN cDNA 2310038H17 gene GeneID:330828 8 5.726 A630085K21 hypothetical protein A630085K21 GeneID:320377 8 5.726 9330175E14Rik RIKEN cDNA 9330175E14 gene GeneID:320587 4 5.72 A230069A22Rik RIKEN cDNA A230069A22 gene GeneID:242805 4 5.72 E230028L10Rik RIKEN cDNA E230028L10 gene GeneID:16534 7 5.7 Kcnn4 IK1, IKCA1, KCA4, SK4, mIKCa1 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 GeneID:74349 7 5.686 4632419K20Rik RIKEN cDNA 4632419K20 gene GeneID:233649 7 5.686 Cnga4 cyclic nucleotide gated channel alpha 4 GeneID:244189 7 5.686 LOC244189 similar to hypothetical protein GeneID:435351 13 5.667 4930482L21Rik RIKEN cDNA 4930482L21 gene GeneID:19766 13 5.667 Ripk1 D330015H01Rik, RIP receptor (TNFRSF)-interacting serine- kinase 1 GeneID:73230 9 5.664 Bmper CV-2, Crim3, Cv2 BMP-binding endothelial regulator GeneID:26891 5 5.655 Cops4 COP9 (constitutive photomorphogenic) homolog, subunit 4 () GeneID:66309 5 5.643 2810021O14Rik RIKEN cDNA 2810021O14 gene GeneID:26456 19 5.643 Sema4g sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G GeneID:94067 19 5.643 Mrpl43 bMRP36a mitochondrial ribosomal protein L43 GeneID:226153 19 5.643 Peo1 MGC41750, PEO progressive external ophthalmoplegia 1 (human) GeneID:226154 19 5.643 Lzts2 LAPSER1, MGC7094, mKIAA1813 , putative tumor suppressor 2 GeneID:78781 6 5.642 Zc3hav1 ZAP zinc finger CCCH type, antiviral 1 GeneID:75909 11 5.633 Tmem49 transmembrane protein 49 GeneID:13557 13 5.633 E2F3b, E2f3a, mKIAA0075 transcription factor 3 GeneID:217864 12 5.629 Rcor1 5730409O11, mKIAA0071 REST corepressor 1 GeneID:16952 19 5.625 Anxa1 Anx-1, Anx-A1 GeneID:66978 17 5.624 Luc7l Luc7 homolog (S. cerevisiae)-like GeneID:383229 17 5.624 LOC383229 similar to hemoglobin alpha chain - slender loris GeneID:621421 17 5.624 LOC621421 hypothetical protein LOC621421 GeneID:432552 11 5.614 LOC432552 similar to Hypothetical protein MGC26988 GeneID:20316 11 5.612 Sdf2 stromal cell derived factor 2 GeneID:72503 11 5.612 2610507B11Rik E1, RP23-185A18.9, mKIAA0100 RIKEN cDNA 2610507B11 gene GeneID:100340 4 5.611 Smpdl3b sphingomyelin phosphodiesterase, acid-like 3B GeneID:622847 1 5.608 LOC622847 similar to ribosomal protein S23 GeneID:71884 1 5.608 Chit1 chitinase 1 (chitotriosidase) GeneID:629639 15 5.601 LOC629639 similar to Isocitrate dehydrogenase [NADP] cytoplasmic (Oxalosuccinate decarboxylase) (IDH) (NADP(+)-specific ICDH) (IDP) GeneID:68614 15 5.601 Letmd1 HCCR-2, HCCR1, MCC-32, Mccr LETM1 domain containing 1 GeneID:12524 16 5.598 Cd86 B7, B7-2, B70, CLS1, MB7, TS/A-2 CD86 antigen GeneID:269999 7 5.594 BC061259 9930124N15 cDNA sequence BC061259 GeneID:233904 7 5.594 BC010250 MGC55143, mKIAA0339 cDNA sequence BC010250 GeneID:100978 5 5.586 Nfxl1 DNABF, GCF, TCF9 nuclear transcription factor, X-box binding-like 1 GeneID:627478 5 5.586 LOC627478 hypothetical protein LOC627478 GeneID:238690 13 5.578 Zfp458 Rslcan-7, mszf59-2 zinc finger protein 458 GeneID:67554 14 5.578 Slc25a30 KMCP1 solute carrier family 25, member 30 GeneID:14782 8 5.575 Gsr glutathione reductase 1 GeneID:17000 6 5.573 Ltbr CD18, LTbetaR, TNF-R-III, TNFCR, TNFR-RPlymphotoxin, TNFR2-RP B receptor, TNFRrp GeneID:12703 16 5.569 Socs1 Cish7, JAB, SOCS-1, SSI-1 suppressor of cytokine signaling 1 GeneID:21959 16 5.569 Tnp2 TP2 transition protein 2 GeneID:19120 16 5.569 Prm3 PX protamine 3 GeneID:19119 16 5.569 Prm2 protamine 2 GeneID:19118 16 5.569 Prm1 protamine 1 GeneID:545369 1 5.563 LOC545369 similar to (L1) GeneID:15500 10 5.551 Hsf2 2 GeneID:55963 11 5.548 Slc1a4 ASCT1, SATT solute carrier family 1 (glutamate/neutral amino acid transporter), member 4 GeneID:22689 7 5.548 Zfp27 mkr-4, mszf76 zinc finger protein 27 GeneID:16819 2 5.535 Lcn2 24p3, NGAL lipocalin 2 GeneID:96979 2 5.535 Ptges2 GBF-1, GBF1, MGC6845, PGE2 2 GeneID:67590 19 5.532 4930521E07Rik RIKEN cDNA 4930521E07 gene GeneID:56454 19 5.532 Aldh18a1 MGC32233 aldehyde dehydrogenase 18 family, member A1 GeneID:101471 7 5.532 AA673488 expressed sequence AA673488 GeneID:54123 7 5.532 Irf7 interferon regulatory factor 7 GeneID:72040 7 5.532 Mucdhl mucin and cadherin like GeneID:319236 7 5.521 9230105E10Rik TRIM5 RIKEN cDNA 9230105E10 gene GeneID:244183 7 5.521 A530023O14Rik RIKEN cDNA A530023O14 gene GeneID:170737 8 5.517 Znrf1 Rnf42, Zrfp1, nin283 zinc and ring finger 1 GeneID:21813 9 5.516 Tgfbr2 DNIIR, RIIDN, TbetaR-II, TbetaRII transforming growth factor, beta receptor II GeneID:211978 12 5.515 Zfyve26 4930465A13, mKIAA0321 zinc finger, FYVE domain containing 26 GeneID:19363 12 5.515 Rad51l1 R51H2, mREC2 RAD51-like 1 (S. cerevisiae) GeneID:621519 19 5.514 LOC621519 region containing PRP31 pre-mRNA processing factor 31 homolog (yeast); RIKEN cDNA 1810009A15 gene GeneID:80889 7 5.501 Mesdc1 mesoderm development candidate 1 GeneID:67943 7 5.501 Mesdc2 MGC25959, mKIAA0081 mesoderm development candiate 2 GeneID:19942 11 5.499 Rpl27 ribosomal protein L27 GeneID:70110 11 5.499 Ifi35 IFP35 interferon-induced protein 35 GeneID:26949 11 5.499 Vat1 VAT-1 vesicle amine transport protein 1 homolog (T californica) GeneID:100465 4 5.493 Mobkl2c MOB3C MOB1, Mps One Binder kinase activator-like 2C (yeast) GeneID:67602 6 5.485 Necap1 mFLJ00061 NECAP endocytosis associated 1 GeneID:106840 5 5.482 AA407659 expressed sequence AA407659 GeneID:216441 10 5.476 C78409 D630010I07 expressed sequence C78409 GeneID:70750 1 5.472 Fvt1 follicular lymphoma variant translocation 1 GeneID:16878 11 5.471 Lif leukemia inhibitory factor GeneID:21788 2 5.465 Tfpi EPI, LACI pathway inhibitor GeneID:67878 5 5.464 Tmem33 db83 transmembrane protein 33 GeneID:100715 13 5.463 Papd4 PAP associated domain containing 4 GeneID:320664 2 5.461 F730031O20Rik RIKEN cDNA F730031O20 gene GeneID:56278 13 5.451 Gkap1 42kDa G kinase anchoring protein 1 GeneID:212398 19 5.45 Frat2 MGC37615 frequently rearranged in advanced T-cell 2 GeneID:22682 19 5.444 Za20d2 zinc finger, A20 domain containing 2 GeneID:622598 9 5.439 LOC622598 similar to zinc finger protein 3 isoform 2 GeneID:67561 9 5.439 Wdr48 mKIAA1449 WD repeat domain 48 GeneID:18023 11 5.435 Nfe2l1 LCR-F1, Lcrf1, NRF1, TCF-11, TCF1nuclear1 factor, erythroid derived 2,-like 1 GeneID:20558 11 5.433 Slfn4 schlafen 4 GeneID:213988 15 5.431 Tnrc6b MGC28739 trinucleotide repeat containing 6b GeneID:58861 X 5.422 Cysltr1 CysLT1R, Cyslt1 cysteinyl receptor 1 GeneID:74748 1 5.408 Slamf8 Blame, SBBI42 SLAM family member 8 GeneID:67680 4 5.402 Sdhb succinate dehydrogenase complex, subunit B, iron sulfur (Ip) GeneID:74772 4 5.402 Atp13a2 ATPase type 13A2 GeneID:69536 9 5.385 Hemk1 HemK methyltransferase family member 1 GeneID:235599 9 5.385 6430571L13Rik RIKEN cDNA 6430571L13 gene GeneID:12394 16 5.38 Runx1 AML1, Pebp2a2 runt related transcription factor 1 GeneID:12209 X 5.378 Brs3 BRS-3 bombesin-like receptor 3 GeneID:72459 X 5.378 Htatsf1 TAT-SF1 HIV TAT specific factor 1 GeneID:56706 3 5.377 Ccnl1 Ccnl, ania-6a cyclin L1 GeneID:110532 10 5.373 Adarb1 ADAR2, RED1 , RNA-specific, B1 GeneID:227157 1 5.372 Mpp4 DLG6 membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) GeneID:74018 1 5.372 Als2 Als2cr6, Alsin, MGC27807, mKIAA1563amyotrophic lateral sclerosis 2 (juvenile) homolog (human) GeneID:17901 1 5.361 Myl1 MLC1f, MLC3f myosin, light polypeptide 1 GeneID:66599 11 5.358 Rad52b Rdm1 RAD52 homolog B (S. cerevisiae) GeneID:627798 9 5.357 LOC627798 similar to tumor protein, translationally-controlled 1 GeneID:56444 12 5.356 Actr10 Actr11, Arp11 ARP10 actin-related protein 10 homolog (S. cerevisiae) GeneID:19167 12 5.356 Psma3 proteasome (prosome, macropain) subunit, alpha type 3 GeneID:319845 9 5.353 E130103I17Rik RIKEN cDNA E130103I17 gene GeneID:15467 5 5.343 Eif2ak1 eukaryotic translation initiation factor 2 alpha kinase 1 GeneID:231872 5 5.343 Jtv1 MGC30261, MGC36346, MGC36522,JTV1 MGC37389 gene GeneID:66729 5 5.343 4921520G13Rik RIKEN cDNA 4921520G13 gene GeneID:20743 19 5.339 Spnb3 mKIAA0302 spectrin beta 3 GeneID:66704 19 5.339 4921506I22Rik RIKEN cDNA 4921506I22 gene GeneID:21943 14 5.335 Tnfsf11 Ly109l, ODF, OPG, OPGL, RANKL tumor necrosis factor (ligand) superfamily, member 11 GeneID:16153 1 5.331 Il10 CSIF interleukin 10 GeneID:12367 8 5.314 Casp3 Apopain, CPP32, Caspase-3, Lice, Ycaspaseama 3 GeneID:408022 8 5.314 MGC86034 MGC86034 hypothetical MGC86034 GeneID:63986 7 5.311 Gmfg glia maturation factor, gamma GeneID:68776 17 5.301 Taf11 28kDa, PRO214, TAF2I, TAFII28 TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor GeneID:224650 17 5.301 Anks1 MGC59413, mKIAA0229 ankyrin repeat and SAM domain containing 1 GeneID:319197 7 5.299 Gpr4 G protein-coupled receptor 4 GeneID:403187 7 5.299 Opa3 optic atrophy 3 (human) GeneID:23807 9 5.297 Arih2 ARI2, TRIAD1, UIP48 ariadne homolog 2 (Drosophila) GeneID:625928 9 5.297 LOC625928 hypothetical protein LOC625928 GeneID:76443 1 5.295 2310016D23Rik RIKEN cDNA 2310016D23 gene GeneID:170740 11 5.289 Zfp287 SKAT-2, Skat2, Znf287, mszf16, mszf74zinc finger protein 287 GeneID:16848 5 5.279 Lfng lunatic fringe gene homolog (Drosophila) GeneID:78339 5 5.279 Ttyh3 tweety homolog 3 (Drosophila) GeneID:108960 6 5.278 Irak2 IRAK-2 interleukin-1 receptor-associated kinase 2 GeneID:18854 9 5.277 Pml Trim19 promyelocytic leukemia GeneID:69106 9 5.277 Stoml1 SLP-1, STORP, UNC-24, WPB72 stomatin-like 1 GeneID:52892 11 5.276 Sco1 SCO cytochrome oxidase deficient homolog 1 (yeast) GeneID:66358 11 5.276 2310004I24Rik MDS006, RP23-67J22.7 RIKEN cDNA 2310004I24 gene GeneID:17428 11 5.273 Mnt Rox max binding protein GeneID:68487 6 5.253 1110007F12Rik RIKEN cDNA 1110007F12 gene GeneID:78412 6 5.253 3110062M04Rik RIKEN cDNA 3110062M04 gene GeneID:66789 3 5.249 Alg14 asparagine-linked 14 homolog (yeast) GeneID:381677 5 5.246 Gm1052 gene model 1052, (NCBI) GeneID:11769 5 5.246 Ap1s1 AP19 adaptor protein complex AP-1, sigma 1 GeneID:192656 4 5.244 Ripk2 CARD3, CARDIAK, CCK, RICK, RIP2receptor (TNFRSF)-interacting serine-threonine kinase 2 GeneID:51796 4 5.239 Srrm1 POP101, Srm160 serine/arginine repetitive matrix 1 GeneID:67464 14 5.238 Entpd4 4930513O14Rik, mKIAA0392 ectonucleoside triphosphate diphosphohydrolase 4 GeneID:71520 11 5.238 Grap GRB2-related adaptor protein GeneID:109901 15 5.229 Ela1 PC-TsF elastase 1, pancreatic GeneID:207839 15 5.229 Galnt6 4632410F13, GalNAc-T6 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 GeneID:244059 7 5.228 Chd2 chromodomain DNA binding protein 2 GeneID:54161 6 5.227 Copg Copg1, MGC66965 coatomer protein complex, subunit gamma GeneID:232210 6 5.227 8430410A17Rik RIKEN cDNA 8430410A17 gene GeneID:626007 7 5.227 LOC626007 similar to upstream transcription factor 2 GeneID:17136 7 5.227 Mag siglec-4a -associated glycoprotein GeneID:66438 7 5.227 Hamp2 HEPC2 hepcidin antimicrobial peptide 2 GeneID:77219 12 5.227 Zadh1 zinc binding alcohol dehydrogenase, domain containing 1 GeneID:107586 2 5.22 Ovol2 M-OVO, M-OVO-A, M-OVO-B, MOVO,ovo-like Ovo2 2 (Drosophila) GeneID:12953 2 5.218 Cry2 cryptochrome 2 (photolyase-like) GeneID:244585 8 5.211 1700047E16Rik 4931437C01, Ftm RIKEN cDNA 1700047E16 gene GeneID:26383 8 5.211 Fto mKIAA1752 fatso GeneID:320509 11 5.203 9930022D16Rik RIKEN cDNA 9930022D16 gene GeneID:13929 11 5.203 X83328 Amz2 EST X83328 GeneID:52815 8 5.194 Ldhd lactate dehydrogenase D GeneID:57437 8 5.175 Golga7 GCP16, GOLGA3AP1, HSPC041 golgi autoantigen, golgin subfamily a, 7 GeneID:66194 15 5.173 Pycrl pyrroline-5-carboxylate reductase-like GeneID:22122 15 5.173 Tsta3 FX tissue specific transplantation antigen P35B GeneID:20218 4 5.172 Khdrbs1 p62 KH domain containing, RNA binding, associated 1 GeneID:320302 3 5.172 Glt28d2 GLT28D1L 28 domain containing 2 GeneID:319865 4 5.169 E130114P18Rik RIKEN cDNA E130114P18 gene GeneID:18027 4 5.169 Nfia NF1-A /A GeneID:68966 14 5.167 1500001L15Rik RIKEN cDNA 1500001L15 gene GeneID:11465 11 5.166 Actg1 Actg, E51, MGC28612 actin, gamma, cytoplasmic 1 GeneID:238021 11 5.166 Fscn2 A930022G03 fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) GeneID:226278 19 5.162 Gpr10 GR3, PrRPR G protein-coupled receptor 10 GeneID:53625 11 5.162 B3gnt1 B3Galt6, Beta3gnt, beta-3Gnt UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 GeneID:85305 8 5.162 Kars LysRS, mKIAA0070 lysyl-tRNA synthetase GeneID:57321 8 5.162 Terf2ip telomeric repeat binding factor 2, interacting protein GeneID:627828 8 5.162 LOC627828 similar to heterogeneous nuclear ribonucleoprotein A3 GeneID:23908 3 5.162 Hs2st1 Hs2st, mKIAA0448 heparan sulfate 2-O-sulfotransferase 1 GeneID:93684 3 5.162 15-Sep 15kDa selenoprotein GeneID:21936 4 5.161 Tnfrsf18 AITR tumor necrosis factor receptor superfamily, member 18 GeneID:22334 14 5.156 Vdac2 Vdac6 voltage-dependent anion channel 2 GeneID:69156 14 5.156 Comtd1 catechol-O-methyltransferase domain containing 1 GeneID:319486 14 5.156 A430057M04Rik RIKEN cDNA A430057M04 gene GeneID:74203 11 5.147 Eif4enif1 Clast4 eukaryotic translation initiation factor 4E nuclear import factor 1 GeneID:54446 8 5.145 Nfat5 CAG-8, CAG80, NFATL1, TonEBP, mKIAA0827,nuclear factor nfatz of activated T-cells 5 GeneID:67459 1 5.137 Nvl nuclear VCP-like GeneID:98417 1 5.137 Cnih4 HSPC163 cornichon homolog 4 (Drosophila) GeneID:384179 5 5.131 LOC384179 similar to ribosomal protein L15 GeneID:16502 7 5.13 Kcnc1 C230009H10Rik, KShIIIB, KV4, Kv3.1,potassium NGK2 voltage gated channel, Shaw-related subfamily, member 1 GeneID:21872 7 5.129 Tjp1 ZO-1, ZO1 tight junction protein 1 GeneID:55932 3 5.128 Gbp4 Gbp3 guanylate nucleotide binding protein 4 GeneID:70560 3 5.125 Wars2 tryptophanyl tRNA synthetase 2 (mitochondrial) GeneID:621836 11 5.125 LOC621836 similar to HYPOTHETICAL PROTEIN ORF-1137 GeneID:68045 14 5.121 2700060E02Rik RIKEN cDNA 2700060E02 gene GeneID:12866 9 5.12 Cox7a2 COX7AL, CoxVIIa-L , subunit VIIa 2 GeneID:69981 9 5.12 Tmem30a transmembrane protein 30A GeneID:216742 11 5.12 A730024A03Rik KIAA1961, RP23-297J14.6, mKIAA1961RIKEN cDNA A730024A03 gene GeneID:192786 11 5.12 Rapgef6 A530068K01, PDZ-GEF2, RA-GEF-2,Rap Ragef2, guanine mKIAA4052 nucleotide exchange factor (GEF) 6 GeneID:109019 1 5.104 5830411E10Rik Nabp1 RIKEN cDNA 5830411E10 gene GeneID:19720 13 5.103 Trim27 tripartite motif protein 27 GeneID:66825 4 5.102 Rnf186 ring finger protein 186 GeneID:77056 4 5.102 Tmco4 ransmembrane and coiled-coil domains 4 GeneID:625817 4 5.102 LOC625817 hypothetical protein LOC625817 GeneID:243382 6 5.101 Ppm1k protein phosphatase 1K (PP2C domain containing) GeneID:22222 2 5.101 Ubr1 ubiquitin protein E3 component n-recognin 1 GeneID:96957 2 5.101 Tmem62 transmembrane protein 62 GeneID:17308 11 5.098 Mgat1 mannoside acetylglucosaminyltransferase 1 GeneID:78896 1 5.096 1500015O10Rik Ecrg4 RIKEN cDNA 1500015O10 gene GeneID:23877 7 5.096 Fiz1 mFLJ00416 Flt3 interacting zinc finger protein 1 GeneID:66056 7 5.096 Zfp524 zinc finger protein 524 GeneID:319748 7 5.096 6430526N21Rik RIKEN cDNA 6430526N21 gene GeneID:270066 8 5.096 6030458H05 hypothetical protein 6030458H05 GeneID:70625 8 5.096 Crsp7 required for Sp1 transcriptional activation, subunit 7 GeneID:17215 1 5.095 Mcm3 P1, p1.m minichromosome maintenance deficient 3 (S. cerevisiae) GeneID:622917 1 5.095 LOC622917 similar to T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta) GeneID:11535 7 5.084 Adm AM adrenomedullin GeneID:27410 17 5.075 Abca3 ABC-C, Abc3 ATP-binding cassette, sub-family A (ABC1), member 3 GeneID:381072 17 5.075 Abca17 ATP-binding cassette, sub-family A (ABC1), member 17 GeneID:623005 8 5.072 LOC623005 similar to CAMP GeneID:16363 8 5.072 Irf2 Irf-2 interferon regulatory factor 2 GeneID:232334 6 5.069 Vgll4 MGC54805, VGL-4 vestigial like 4 (Drosophila) GeneID:72027 15 5.067 Slc39a4 AWMS2, ZIP4 solute carrier family 39 (zinc transporter), member 4 GeneID:66914 15 5.067 Vps28 vacuolar protein sorting 28 (yeast) GeneID:72749 15 5.067 Nfkbil2 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 GeneID:14043 2 5.066 Ext2 exostoses (multiple) 2 GeneID:108154 13 5.064 Adamts6 5031426K13, ADAM-TS6 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 6 GeneID:18787 5 5.062 Serpine1 PAI-1, PAI1 serine (or cysteine) peptidase inhibitor, clade E, member 1 GeneID:69181 10 5.061 Dyrk2 1810038L18Rik dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 GeneID:328234 13 5.06 Rnf182 ring finger protein 182 GeneID:622586 14 5.059 LOC622586 hypothetical protein LOC622586 GeneID:626189 14 5.059 LOC626189 similar to zinc finger protein 277 isoform 1 GeneID:625673 10 5.059 LOC625673 hypothetical protein LOC625673 GeneID:18245 10 5.059 Oaz1 AZ-1, Antizyme, ODC-Az, Oaz ornithine decarboxylase antizyme GeneID:237403 10 5.059 LOC237403 similar to hypothetical protein FLJ31810 GeneID:52857 7 5.058 D7Bwg0611e DNA segment, Chr 7, Brigham & Women's Genetics 0611 expressed GeneID:629974 11 5.052 LOC629974 similar to open reading frame 27 GeneID:75345 1 5.047 Slamf7 19A, 19A24, CRACC, CS1 SLAM family member 7 GeneID:50917 8 5.042 Galns mFLJ00319 galactosamine (N-acetyl)-6-sulfate sulfatase GeneID:59005 8 5.042 1810017G16Rik Hspc176 RIKEN cDNA 1810017G16 gene GeneID:382035 8 5.042 Pabpnl1 ePABP2 poly(A)binding protein nuclear-like 1 GeneID:74315 11 5.037 3732413I11Rik RP23-103H9.1, TMRF1 RIKEN cDNA 3732413I11 gene GeneID:232989 7 5.036 Hnrpul1 E1B-AP5, E1BAP5, MGC36621 heterogeneous nuclear ribonucleoprotein U-like 1 GeneID:26362 7 5.036 Axl Ark, Tyro7 AXL receptor tyrosine kinase GeneID:66314 2 5.034 Tpd52l2 D54 tumor protein D52-like 2 GeneID:241850 2 5.034 1700095H12 hypothetical protein 1700095H12 GeneID:71365 10 5.03 5430420P03Rik Plmp RIKEN cDNA 5430420P03 gene GeneID:230784 4 5.028 Sesn2 HI95, MGC11758, SEST2, Ses2 sestrin 2 GeneID:214162 9 5.025 Mll 6430520K01, ALL-1, Cxxc7, HRX, HTRX1,myeloid/lymphoid mKIAA4050 or mixed-lineage leukemia GeneID:16475 14 5.022 Jub ajuba GeneID:74359 14 5.022 4931414P19Rik RIKEN cDNA 4931414P19 gene GeneID:230398 4 5.022 Ifna6T interferon alpha 6T GeneID:15965 4 5.022 Ifna2 interferon alpha family, gene 2 GeneID:15974 4 5.022 Ifnab interferon alpha family, gene B GeneID:72238 17 5.021 Tbc1d5 mKIAA0210 TBC1 domain family, member 5 GeneID:19325 12 5.019 Rab10 RAB10, member RAS oncogene family GeneID:234421 8 5.016 Cib3 KIP3 calcium and integrin binding family member 3 GeneID:67922 8 5.016 2510049I19Rik RIKEN cDNA 2510049I19 gene GeneID:21858 11 5.016 Timp2 tissue inhibitor of metalloproteinase 2 GeneID:12773 9 5.011 Ccr4 LESTR chemokine (C-C motif) receptor 4 GeneID:67429 15 5.008 Nudcd1 CML66-L, Cml66 NudC domain containing 1 GeneID:223527 15 5.008 Eny2 6720481I12, DC6, Ey2 enhancer of yellow 2 homolog (Drosophila) GeneID:68758 5 5.008 Abhd11 abhydrolase domain containing 11 GeneID:21941 4 5.006 Tnfrsf8 D1S166E, Ki, Ki-1 tumor necrosis factor receptor superfamily, member 8 GeneID:319370 11 5.005 1110014K08Rik RIKEN cDNA 1110014K08 gene GeneID:217341 11 5.005 Gm66 gene model 66, (NCBI) GeneID:317750 2 5.004 Slc24a5 JSX, NCX5 solute carrier family 24, member 5 GeneID:16423 16 5.002 Cd47 IAP CD47 antigen (Rh-related antigen, integrin-associated signal transducer) GeneID:64291 18 5.002 Osbpl1a Osbpl1b oxysterol binding protein-like 1A GeneID:24116 5 4.997 Whsc2 NELFA Wolf-Hirschhorn syndrome candidate 2 (human) GeneID:104709 11 4.996 BB220380 expressed sequence BB220380 GeneID:215723 11 4.996 BC024997 MGC32231 cDNA sequence BC024997 GeneID:432442 10 4.995 Akap7 6430401D08, AKAP15, Akap18 A kinase (PRKA) anchor protein 7 GeneID:56631 11 4.986 Trim17 terf tripartite motif protein 17 GeneID:78303 11 4.986 Hist3h2ba histone 3, H2ba GeneID:382522 11 4.986 Hist3h2bb 4933432H21, B2Hist3h2bb histone 3, H2bb GeneID:319162 11 4.986 Hist3h2a histone 3, H2a GeneID:382523 11 4.986 LOC382523 similar to histone 1, H3g GeneID:12370 1 4.984 Casp8 Caspase-8, FLICE, MACH, Mch5 caspase 8 GeneID:17752 8 4.977 Mt4 MT-IV metallothionein 4 GeneID:218203 13 4.975 Mylip MGC11702, Mir myosin regulatory light chain interacting protein GeneID:545570 3 4.974 LOC545570 similar to hypothetical protein LOC66976 GeneID:66360 19 4.964 2310002J21Rik RIKEN cDNA 2310002J21 gene GeneID:56392 19 4.964 Shoc2 Sur-8, Sur8, mKIAA0862 soc-2 (suppressor of clear) homolog (C. elegans) GeneID:240793 1 4.963 Gm201 gene model 201, (NCBI) GeneID:52705 10 4.962 Hrb2 HIV-1 Rev binding protein 2 GeneID:73690 10 4.962 Glipr1 RTVP-1, RTVP1, mRTVP-1 GLI pathogenesis-related 1 (glioma) GeneID:629232 10 4.962 LOC629232 similar to calcyphosphine 2 GeneID:67266 5 4.956 2900024C23Rik RIKEN cDNA 2900024C23 gene GeneID:27397 7 4.955 Mrpl17 MRP-L26 mitochondrial ribosomal protein L17 GeneID:319997 1 4.95 A630001G21Rik RIKEN cDNA A630001G21 gene GeneID:71166 1 4.947 4933424G06Rik RIKEN cDNA 4933424G06 gene GeneID:22637 1 4.947 Zap70 70kDa, TZK, ZAP-70 zeta-chain (TCR) associated protein kinase GeneID:83925 15 4.942 Trps1 MGC46754 trichorhinophalangeal syndrome I (human) GeneID:17085 1 4.94 Ly9 CD229, Lgp100, SLAMF3, T100 lymphocyte antigen 9 GeneID:20851 11 4.93 Stat5b signal transducer and activator of transcription 5B GeneID:622444 5 4.921 LOC622444 hypothetical protein LOC622444 GeneID:24055 5 4.921 Sh3bp2 3BP2 SH3-domain binding protein 2 GeneID:230259 4 4.918 E130308A19Rik MGC47072 RIKEN cDNA E130308A19 gene GeneID:72495 7 4.917 2610206C17Rik RIKEN cDNA 2610206C17 gene GeneID:58207 2 4.916 Slc43a3 SEEEG-1 solute carrier family 43, member 3 GeneID:17750 8 4.916 Mt2 MT-II metallothionein 2 GeneID:17748 8 4.916 Mt1 MT-I metallothionein 1 GeneID:30941 1 4.916 Usp21 Usp16 ubiquitin specific peptidase 21 GeneID:66155 1 4.916 Ufc1 ubiquitin-fold modifier conjugating enzyme 1 GeneID:622236 1 4.916 LOC622236 similar to ribosomal protein L27 GeneID:26447 18 4.916 Poli polymerase (DNA directed), iota GeneID:140580 13 4.913 Elmo1 CED-12, mKIAA0281 engulfment and cell motility 1, ced-12 homolog (C. elegans) GeneID:17248 1 4.892 Mdm4 Mdmx transformed mouse 3T3 cell double minute 4 GeneID:622475 1 4.892 LOC622475 similar to ribosomal protein S8 GeneID:226169 19 4.887 Pprc1 A930018M19, MGC12121, PRC, mKIAA0595peroxisome proliferative activated receptor, gamma, coactivator-related 1 GeneID:257754 7 4.887 GA_x5J8B7W72BC-125691-125311 olfactory receptor GA_x5J8B7W72BC-125691-125311 GeneID:258512 7 4.887 Olfr530 MOR252-2 olfactory receptor 530 GeneID:13610 13 4.882 Edg3 LPb3, S1P3 endothelial differentiation, sphingolipid G-protein-coupled receptor, 3 GeneID:66934 2 4.882 1700022L09Rik RIKEN cDNA 1700022L09 gene GeneID:72477 2 4.874 2610301K12Rik RIKEN cDNA 2610301K12 gene GeneID:76630 19 4.872 1700095N21Rik ALMalpha, AMSH-FP RIKEN cDNA 1700095N21 gene GeneID:68024 13 4.872 Hist1h2bc histone 1, H2bc GeneID:107970 13 4.872 Hist1h1t H1t histone 1, H1t GeneID:319155 13 4.872 Hist1h4c histone 1, H4c GeneID:319164 13 4.872 Hist1h2ac 9030420B16 histone 1, H2ac GeneID:270166 9 4.87 Clpx E330029I21 caseinolytic peptidase X (E.coli) GeneID:58222 11 4.868 Rab37 RAB37, member of RAS oncogene family GeneID:26941 11 4.868 Slc9a3r1 EBP-50, NHE-RF, NHERF-1 solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1 GeneID:14536 2 4.868 Nr6a1 NCNF, RTR nuclear receptor subfamily 6, group A, member 1 GeneID:26423 2 4.868 Nr5a1 Ad4BP, ELP, ELP-3, Ftz-F1, SF-1, SF1nuclear receptor subfamily 5, group A, member 1 GeneID:624625 2 4.868 LOC624625 hypothetical protein LOC624625 GeneID:73178 6 4.863 Wasl N-WASP Wiskott-Aldrich syndrome-like (human) GeneID:74387 16 4.862 4932438H23Rik 4931406G06Rik RIKEN cDNA 4932438H23 gene GeneID:79560 11 4.858 Ublcp1 MGC7513 ubiquitin-like domain containing CTD phosphatase 1 GeneID:71701 11 4.852 Pnpt1 Old35, PNPase, RP23-313I4.2 polyribonucleotide nucleotidyltransferase 1 GeneID:108686 11 4.843 A430106J12Rik Ape, Giv, Hkrp1, RP23-92B18.2 RIKEN cDNA A430106J12 gene GeneID:67939 11 4.843 2010316F05Rik RP23-92B18.3 RIKEN cDNA 2010316F05 gene GeneID:16190 7 4.843 Il4ra CD124 interleukin 4 receptor, alpha GeneID:228829 2 4.842 Phf20 PHD finger protein 20 GeneID:19018 2 4.842 Scand1 Leap1, PGC-2 SCAN domain-containing 1 GeneID:628702 2 4.842 LOC628702 similar to 60S ribosomal protein L37a GeneID:19647 7 4.835 Rbbp6 MGC32263 retinoblastoma binding protein 6 GeneID:67579 11 4.835 Cpeb4 mKIAA1673 cytoplasmic element binding protein 4 GeneID:218314 13 4.835 A230042K10Rik Rslcan23 RIKEN cDNA A230042K10 gene GeneID:110651 X 4.83 Rps6ka3 MPK-9, Rsk2, S6K-alpha3, pp90RSK2ribosomal protein S6 kinase polypeptide 3 GeneID:546828 4 4.829 LOC546828 similar to SMT3 suppressor of mif two 3 homolog 2 GeneID:14319 19 4.828 Fth1 FHC, Fth, MFH ferritin heavy chain 1 GeneID:24115 19 4.828 Vmd2 BMD, mBest1 vitelliform macular dystrophy 2 homolog (human) GeneID:50778 1 4.824 Rgs1 BL34 regulator of G-protein signaling 1 GeneID:67547 3 4.824 Slc39a8 BIGM103 solute carrier family 39 (metal ion transporter), member 8 GeneID:229521 3 4.821 Syt11 1500004A13Rik, 3632445O20Rik, 5430404N14Riksynaptotagmin XI GeneID:230806 4 4.821 Aim1l absent in melanoma 1-like GeneID:23833 4 4.821 Cd52 B7, B7-Ag, CAMPATH-1, CLS1, MB7CD52 antigen GeneID:67586 4 4.821 Ubxd5 Soc, Soci UBX domain containing 5 GeneID:227059 1 4.82 Slc39a10 5430433I10, mKIAA1265 solute carrier family 39 (zinc transporter), member 10 GeneID:245240 11 4.814 9930111J21Rik RIKEN cDNA 9930111J21 gene GeneID:68539 19 4.813 1110006I15Rik RIKEN cDNA 1110006I15 gene GeneID:16612 7 4.811 Klk6 Klk1, Klk1b6, mGk-6 kallikrein 6 GeneID:70004 7 4.811 1700028J19Rik RIKEN cDNA 1700028J19 gene GeneID:66976 7 4.811 2410002F23Rik RIKEN cDNA 2410002F23 gene GeneID:11951 11 4.811 Atp5g1 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1 GeneID:17189 15 4.811 Mb myoglobin GeneID:71939 15 4.811 Apol6 apolipoprotein L, 6 GeneID:27360 19 4.809 Add3 adducin 3 (gamma) GeneID:80890 3 4.808 Trim2 mKIAA0517, narf tripartite motif protein 2 GeneID:624713 3 4.808 LOC624713 similar to large subunit ribosomal protein L36a GeneID:230125 4 4.803 Mcart1 mitochondrial carrier triple repeat 1 GeneID:624202 4 4.803 LOC624202 hypothetical protein LOC624202 GeneID:15463 1 4.791 Hrb , Rip HIV-1 Rev binding protein GeneID:22030 2 4.79 Traf2 Tnf receptor-associated factor 2 GeneID:620586 2 4.79 LOC620586 hypothetical protein LOC620586 GeneID:59022 2 4.79 Edf1 endothelial differentiation-related factor 1 GeneID:21355 17 4.79 Tap2 ABC18, APT2, MTP2, PSF2, RING11,transporter Y1 2, ATP-binding cassette, sub-family B (MDR/TAP) GeneID:18390 10 4.786 Oprm1 MOR-1, Oprm, mor, muOR, receptoropioid receptor, mu 1 GeneID:228866 2 4.783 F730014I05Rik 4930445K14Rik, C20orf67, MGC25976,RIKEN PCIF1 cDNA F730014I05 gene GeneID:74685 16 4.783 4930451C15Rik mCG12354, mCP17390 RIKEN cDNA 4930451C15 gene GeneID:18440 16 4.783 P2rxl1 P2xm purinergic receptor P2X-like 1, orphan receptor GeneID:224022 16 4.783 Slc7a4 MGC27672 solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 GeneID:70025 4 4.778 Acot7 ACH1, ACT, CTE-II, LACH1 acyl-CoA thioesterase 7 GeneID:93736 11 4.776 Aff4 AF5Q31, Alf4, MCEF AF4/FMR2 family, member 4 GeneID:259301 11 4.776 Leap2 leap-2 liver-expressed antimicrobial peptide 2 GeneID:22272 11 4.776 1500040F11Rik 9.5kDa, QP-C, Qpc, Uqcrb RIKEN cDNA 1500040F11 gene GeneID:14566 11 4.776 Gdf9 growth differentiation factor 9 GeneID:22779 1 4.771 Zfpn1a2 Znfn1a2 zinc finger protein, subfamily 1A, 2 (Helios) GeneID:110355 19 4.767 Adrbk1 GRK2, betaARK1 adrenergic receptor kinase, beta 1 GeneID:17260 13 4.767 Mef2c myocyte enhancer factor 2C GeneID:623124 13 4.767 LOC623124 hypothetical protein LOC623124 GeneID:545812 5 4.765 LOC545812 similar to paired immunoglobin-like type 2 receptor beta GeneID:17755 13 4.765 Mtap1b A230055D22, LC1, MAP1B, MAP5 -associated protein 1 B GeneID:258836 4 4.764 Olfr272 MOR262-7 olfactory receptor 272 GeneID:623777 5 4.764 LOC623777 similar to CG40351-PA.3 GeneID:208043 5 4.764 BC035291 MGC39035, mKIAA1076 cDNA sequence BC035291 GeneID:244418 8 4.761 D8Ertd82e 9830148H23, mFLJ00269 DNA segment, Chr 8, ERATO Doi 82, expressed GeneID:627936 6 4.757 LOC627936 similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) GeneID:209589 6 4.757 LOC209589 similar to (S8) GeneID:209590 6 4.757 Il23r IL-23R interleukin 23 receptor GeneID:625840 5 4.754 LOC625840 hypothetical protein LOC625840 GeneID:545732 5 4.754 LOC545732 Speer1-like GeneID:384144 5 4.754 LOC384144 similar to Anaphase promoting complex subunit 5 (APC5) (Cyclosome subunit 5) GeneID:72542 5 4.75 2610528A17Rik RIKEN cDNA 2610528A17 gene GeneID:19301 5 4.75 Pxmp2 22kDa, PMP22 peroxisomal membrane protein 2 GeneID:64213 6 4.748 St7 HELG, RAY1, SEN4, TSG7 Suppression of tumorigenicity 7 GeneID:241158 1 4.741 Ankmy1 ZMYND13 ankyrin repeat and MYND domain containing 1 GeneID:67446 1 4.741 0710001B24Rik RIKEN cDNA 0710001B24 gene GeneID:108657 1 4.741 Rnpepl1 arginyl ()-like 1 GeneID:66091 7 4.738 Ndufa3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 GeneID:69714 7 4.738 Tfpt Amida, FB1 TCF3 (E2A) fusion partner GeneID:232790 7 4.738 Oscar mOSCAR, mOSCAR-M1, mOSCAR-M2,osteoclast mOSCAR-M3 associated receptor GeneID:236792 X 4.737 Tmem32 MGC40705 transmembrane protein 32 GeneID:24015 8 4.734 Abce1 Oabp, RLI, RNS41 ATP-binding cassette, sub-family E (OABP), member 1 GeneID:73945 8 4.734 Otud4 mKIAA1046 OTU domain containing 4 GeneID:107730 8 4.734 Tpd52-ps tumor protein D52, pseudogene GeneID:238393 12 4.732 BC049975 cDNA sequence BC049975 GeneID:628891 12 4.732 LOC628891 similar to developmentally regulated GTP binding protein 2 GeneID:13498 6 4.732 Atn1 Atr1, atrophin-1 atrophin 1 GeneID:16193 5 4.731 Il6 interleukin 6 GeneID:12444 6 4.724 Ccnd2 cyclin D2 GeneID:16408 7 4.722 Itgal LFA-1 GeneID:14585 19 4.72 Gfra1 glial cell line derived neurotrophic factor family receptor alpha 1 GeneID:54698 9 4.704 Crtam cytotoxic and molecule GeneID:72828 9 4.704 2810457I06Rik p70 RIKEN cDNA 2810457I06 gene GeneID:66193 4 4.701 1110049F12Rik Trp26 RIKEN cDNA 1110049F12 gene GeneID:72404 X 4.698 Wdr44 9130203L06, DKFZp686L20145, RAB1WD1BP repeat, RPH1 domain1 44 GeneID:385247 15 4.694 LOC385247 similar to UBASH3A protein GeneID:14583 6 4.693 Gfpt1 GFA, GFAT, GFAT1, GFAT1m, Gfpt glutamine fructose-6-phosphate transaminase 1 GeneID:56748 6 4.693 Hirip5 CGI-33 histone regulation defective interacting protein 5 GeneID:497210 6 4.693 Tpt1p Tpt1 pseudogene GeneID:52906 10 4.693 Ahi1 Abelson helper integration site GeneID:628313 11 4.69 LOC628313 hypothetical protein LOC628313 GeneID:14312 17 4.686 Brd2 Frg-1, NAT, Ring3, mKIAA4005 bromodomain containing 2 GeneID:225872 19 4.685 Npas4 LE-PAS neuronal PAS domain protein 4 GeneID:74455 2 4.678 Nsun6 NOPD1 NOL1/NOP2/Sun domain family 6 GeneID:75869 2 4.678 Arl8 ADP-ribosylation factor-like 8 GeneID:106039 15 4.677 Gga1 golgi associated, gamma adaptin ear containing, ARF binding protein 1 GeneID:20401 15 4.677 Sh3bp1 3BP-1 SH3-domain binding protein 1 GeneID:67200 6 4.675 2700091N06Rik RIKEN cDNA 2700091N06 gene GeneID:214899 6 4.675 Jarid1a MGC11659, RBP2 jumonji, AT rich interactive domain 1A (Rbp2 like) GeneID:108943 3 4.671 Rg9mtd2 Rnmtd2 RNA (guanine-9-) methyltransferase domain containing 2 GeneID:53621 6 4.67 Cnot4 Not4h, Not4hp CCR4-NOT transcription complex, subunit 4 GeneID:227656 2 4.668 Rexo4 REX4, RNA exonuclease 4 homolog (S. cerevisiae) GeneID:279028 2 4.668 Adamts13 vWF-CP a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 GeneID:15251 12 4.667 Hif1a HIF-1alpha, MOP1 hypoxia inducible factor 1, alpha subunit GeneID:103161 10 4.665 Apof apolipoprotein F GeneID:20847 10 4.665 Stat2 signal transducer and activator of transcription 2 GeneID:66185 4 4.662 1110037F02Rik mKIAA1429 RIKEN cDNA 1110037F02 gene GeneID:50772 9 4.661 Mapk6 2610021I23Rik, Mapk63, Prkm6 mitogen-activated protein kinase 6 GeneID:228543 2 4.658 Rhov MGC29297 ras homolog gene family, member V GeneID:69053 16 4.658 1810013L24Rik RIKEN cDNA 1810013L24 gene GeneID:16362 11 4.655 Irf1 interferon regulatory factor 1 GeneID:319544 11 4.655 B430217B02Rik RIKEN cDNA B430217B02 gene GeneID:74343 3 4.654 4632407F12Rik Torc2 RIKEN cDNA 4632407F12 gene GeneID:30791 3 4.654 Slc39a1 Zip1 solute carrier family 39 (zinc transporter), member 1 GeneID:78284 3 4.654 Creb3l4 AIBZIP, Atce1, CREB3, CREB4, JAL,cAMP TISP40 responsive element binding protein 3-like 4 GeneID:114604 16 4.649 Prdm15 C21orf83 PR domain containing 15 GeneID:68021 13 4.648 Bphl biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) GeneID:22151 13 4.648 Tubb2 M(beta)2, Tubb2 tubulin, beta 2 GeneID:18706 3 4.645 Pik3ca caPI3K, p110 phosphatidylinositol 3-kinase, catalytic, alpha polypeptide GeneID:626862 2 4.644 LOC626862 similar to MTH2 protein GeneID:212670 2 4.639 Catsper2 cation channel, sperm associated 2 GeneID:14827 2 4.639 Pdia3 58kDa, ERp57, ERp60, ERp61, PDI,protein PDI-Q2, disulfide PI-PLC, isomerase PLC[a] associated 3 GeneID:320718 1 4.637 Slc26a9 solute carrier family 26, member 9 GeneID:77022 17 4.637 2700099C18Rik RIKEN cDNA 2700099C18 gene GeneID:627644 17 4.637 LOC627644 similar to HEC protein GeneID:76781 17 4.637 Mettl4 A730091E08Rik, HsT661 methyltransferase like 4 GeneID:30795 12 4.635 Fkbp3 25kDa, FKBP25 FK506 binding protein 3 GeneID:104806 12 4.635 Fancm Fanconi anemia, complementation group M GeneID:14581 5 4.631 Gfi1 growth factor independent 1 GeneID:627933 10 4.63 LOC627933 hypothetical protein LOC627933 GeneID:26408 10 4.63 Map3k5 ASK1, MAPKKK5 mitogen activated protein kinase kinase kinase 5 GeneID:210145 7 4.63 Irgc1 immunity-related GTPase family, cinema 1 GeneID:230393 4 4.628 BC057079 mKIAA1797 cDNA sequence BC057079 GeneID:22778 11 4.623 Zfpn1a1 LyF-1, Znfn1a1, hlk-1, mKIAA4227 zinc finger protein, subfamily 1A, 1 (Ikaros) GeneID:17210 3 4.619 Mcl1 myeloid cell leukemia sequence 1 GeneID:140810 2 4.618 Ttbk1 TTK, Ttbk, mKIAA0847 tau tubulin kinase 1 GeneID:233033 7 4.616 BC042901 MGC30302 cDNA sequence BC042901 GeneID:65969 2 4.615 Cubn cubilin (intrinsic factor-cobalamin receptor) GeneID:52589 15 4.614 Ncald delta GeneID:381122 17 4.613 Gm943 gene model 943, (NCBI) GeneID:54402 17 4.609 Stk19 G11, RP1 serine/threonine kinase 19 GeneID:112403 17 4.609 Dom3z NG6 DOM-3 homolog Z (C. elegans) GeneID:108077 17 4.609 Skiv2l SKI, Ski2w superkiller viralicidic activity 2-like (S. cerevisiae ) GeneID:18021 8 4.609 Nfatc3 NFAT4, NFATx nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 GeneID:77411 8 4.609 Rbm35b RNA binding motif protein 35b GeneID:19354 15 4.608 Rac2 RAS-related C3 botulinum 2 GeneID:626952 15 4.608 LOC626952 similar to Eso3 protein GeneID:70511 16 4.605 5730409G15Rik RIKEN cDNA 5730409G15 gene GeneID:13139 10 4.604 Dgka 80kDa diacylglycerol kinase, alpha GeneID:78428 10 4.604 Wibg Pym within bgcn homolog (Drosophila) GeneID:330192 5 4.602 Vps37b FLJ12750, MGC25614 vacuolar protein sorting 37B (yeast) GeneID:13433 9 4.6 Dnmt1 Cxxc9, Dnmt, Dnmt1o, MTase DNA methyltransferase (cytosine-5) 1 GeneID:14739 9 4.6 Edg5 H218, LPb2, S1P2 endothelial differentiation, sphingolipid G-protein-coupled receptor, 5 GeneID:12068 6 4.597 Bet1 Bet-1 blocked early in transport 1 homolog (S. cerevisiae) GeneID:13132 15 4.592 Dab2 Doc-2, Doc2, p96 disabled homolog 2 (Drosophila) GeneID:380950 15 4.592 D630005B22Rik RIKEN cDNA D630005B22 gene GeneID:78798 17 4.592 Eml4 echinoderm microtubule associated protein like 4 GeneID:97122 3 4.586 Hist2h4 H4 histone 2, H4 GeneID:319154 3 4.586 Hist2h3b H3-616 histone 2, H3b GeneID:319189 3 4.586 Hist2h2bb H2b-616 histone 2, H2bb GeneID:213391 6 4.585 Rassf4 AD037 Ras association (RalGDS/AF-6) domain family 4 GeneID:17149 4 4.584 Magoh mago-nashi homolog, proliferation-associated (Drosophila) GeneID:74098 4 4.584 0610037L13Rik RIKEN cDNA 0610037L13 gene GeneID:13057 8 4.583 Cyba b558 cytochrome b-245, alpha polypeptide GeneID:192156 8 4.583 Mvd mevalonate (diphospho) decarboxylase GeneID:319574 8 4.583 9330133O14Rik RIKEN cDNA 9330133O14 gene GeneID:102141 8 4.583 Snx25 SBBI31 sorting nexin 25 GeneID:12166 14 4.578 Bmpr1a ALK3, BMPR-IA bone morphogenetic protein receptor, type 1A GeneID:140559 1 4.569 Igsf8 EWI-2, PGRL immunoglobulin superfamily, member 8 GeneID:216724 11 4.567 Rufy1 Rabip4, ZFYVE12 RUN and FYVE domain containing 1 GeneID:17869 15 4.566 Myc Myc2, c-myc myelocytomatosis oncogene GeneID:217732 12 4.565 2310044G17Rik mKIAA1737 RIKEN cDNA 2310044G17 gene GeneID:13067 2 4.562 Cyct cytochrome c, testis GeneID:241490 2 4.562 Drbp1 Drb1 developmentally regulated RNA binding protein 1 GeneID:18438 5 4.559 P2rx4 P2X4 purinergic receptor P2X, ligand-gated ion channel 4 GeneID:16164 X 4.556 Il13ra1 CD213a1, IL-13r[a], Il13ra, NR4 interleukin 13 receptor, alpha 1 GeneID:72446 2 4.555 2600010E01Rik RIKEN cDNA 2600010E01 gene GeneID:12400 8 4.554 Cbfb PEA2, PEBP2b, Pebp2 beta GeneID:67039 12 4.549 2600011C06Rik RBM25, RNPC7 RIKEN cDNA 2600011C06 gene GeneID:19164 12 4.549 Psen1 PS-1, PS1, S182 presenilin 1 GeneID:60425 19 4.548 Doc2g double C2, gamma GeneID:17995 19 4.548 Ndufv1 NADH dehydrogenase (ubiquinone) flavoprotein 1 GeneID:109575 19 4.548 Tbx10 Tbx13, Tbx7 T-box 10 GeneID:73788 19 4.548 4833408A19Rik RIKEN cDNA 4833408A19 gene GeneID:66387 19 4.548 Nudt8 nudix (nucleoside diphosphate linked moiety X)-type motif 8 GeneID:16491 3 4.546 Kcna3 Kv1.3 potassium voltage-gated channel, shaker-related subfamily, member 3 GeneID:229694 3 4.546 AI504432 A230102G19 expressed sequence AI504432 GeneID:239647 15 4.546 MGC47262 hypothetical protein MGC47262 GeneID:105827 15 4.546 Amigo2 Ali1 adhesion molecule with Ig like domain 2 GeneID:209212 4 4.541 BC010311 MGC11786 cDNA sequence BC010311 GeneID:71890 4 4.539 Mad2l2 MAD2B, REV7 mitotic arrest deficient-like 2 (yeast) GeneID:50762 4 4.539 Fbxo6b FBG2, Fbs2, Fbx6b F-box only protein 6b GeneID:230903 4 4.539 Fbxo44 5730411K09, FBG3, FBX30, Fbx6a : F-box protein 44 GeneID:230904 4 4.539 Fbxo2 FBG1, FBX2, Fbs1, Fbs2, MGC54895,F-box NFB42 only protein 2 GeneID:243912 7 4.535 Hspb6 Hsp20 heat shock protein, alpha-crystallin-related, B6 GeneID:75660 7 4.535 1810054G18Rik RIKEN cDNA 1810054G18 gene GeneID:66340 7 4.535 Psenen presenilin enhancer 2 homolog (C. elegans) GeneID:333193 7 4.535 BC053749 MGC59495 cDNA sequence BC053749 GeneID:67778 3 4.534 Zfp639 ANC-2H01, ZASC1 zinc finger protein 639 GeneID:20788 15 4.533 Srebf2 SREBP-2, SREBP2, SREBP2gc sterol regulatory element binding factor 2 GeneID:71833 11 4.532 Wdr68 HAN11 WD repeat domain 68 GeneID:70207 11 4.532 2310066I18Rik RIKEN cDNA 2310066I18 gene GeneID:18950 14 4.532 Pnp purine-nucleoside GeneID:22235 5 4.528 Ugdh Udpgdh UDP-glucose dehydrogenase GeneID:20931 2 4.527 Surf2 surfeit gene 2 GeneID:20932 2 4.527 Surf4 surfeit gene 4 GeneID:12258 2 4.522 Serping1 C1INH serine (or cysteine) peptidase inhibitor, clade G, member 1 GeneID:70839 3 4.518 P2ry12 P2Y12 purinergic receptor P2Y, G-protein coupled 12 GeneID:623598 3 4.518 LOC623598 similar to mediator of RNA polymerase II transcription, subunit 12 homolog GeneID:19206 13 4.518 Ptch1 Ptc, Ptc1, Ptch patched homolog 1 GeneID:245651 X 4.516 LOC245651 similar to coiled-coil-helix-coiled-coil-helix domain containing 2 GeneID:234366 8 4.509 Gatad2a MGC29315, MGC36975 GATA zinc finger domain containing 2A GeneID:74150 1 4.507 Slc35f5 solute carrier family 35, member F5 GeneID:319184 13 4.507 Hist1h2bk RP23-9O16.11 histone 1, H2bk GeneID:319158 13 4.507 Hist1h4i histone 1, H4i GeneID:319167 13 4.507 Hist1h2ag histone 1, H2ag GeneID:319183 13 4.507 Hist1h2bj histone 1, H2bj GeneID:319168 13 4.507 Hist1h2ah RP23-9O16.9 histone 1, H2ah GeneID:70909 2 4.504 4921504E06Rik RIKEN cDNA 4921504E06 gene GeneID:621893 3 4.503 LOC621893 similar to GeneID:319176 3 4.503 Hist2h2ac H2a-613b histone 2, H2ac GeneID:319190 3 4.503 Hist2h2be H2b-613 histone 2, H2be GeneID:66580 2 4.503 2610101J03Rik ABTAP RIKEN cDNA 2610101J03 gene GeneID:69487 2 4.503 2310003L22Rik RIKEN cDNA 2310003L22 gene GeneID:15170 6 4.502 Ptpn6 Ptp1C, SHP-1, hcp, motheaten protein tyrosine phosphatase, non-receptor type 6 GeneID:14790 6 4.502 Grcc10 C10 gene rich cluster, C10 gene GeneID:70974 7 4.496 Pgm2l1 BM32A phosphoglucomutase 2-like 1 GeneID:320452 7 4.496 P4ha3 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III GeneID:12946 1 4.493 Crry mCRY complement receptor related protein GeneID:12902 1 4.493 Cr2 C3DR, CD21, CD35 GeneID:65256 12 4.491 Asb2 ankyrin repeat and SOCS box-containing protein 2 GeneID:78895 15 4.491 3000003F02Rik RIKEN cDNA 3000003F02 gene GeneID:266632 15 4.491 Irak4 IRAK-4, NY-REN-64 interleukin-1 receptor-associated kinase 4 GeneID:229007 2 4.489 Zgpat 1500006I01Rik, MGC25688, MGC38468zinc finger, CCCH-type with G patch domain GeneID:72699 2 4.489 Lime1 LIME Lck interacting transmembrane adaptor 1 GeneID:67899 9 4.484 2010110K16Rik RIKEN cDNA 2010110K16 gene GeneID:208650 16 4.484 Cblb Cbl-b Casitas B-lineage lymphoma b GeneID:70998 X 4.476 Phf6 mKIAA1823 PHD finger protein 6 GeneID:20230 17 4.474 Satb1 special AT-rich sequence binding protein 1 GeneID:628919 17 4.474 LOC628919 similar to shroom GeneID:628926 17 4.474 LOC628926 hypothetical protein LOC628926 GeneID:16162 6 4.474 Il12rb2 IL-12RB2 interleukin 12 receptor, beta 2 GeneID:11518 5 4.46 Add1 adducin 1 (alpha) GeneID:68294 5 4.46 0610009O03Rik Tetran RIKEN cDNA 0610009O03 gene GeneID:75416 5 4.46 2610033H07Rik RIKEN cDNA 2610033H07 gene GeneID:214359 4 4.459 Tmem51 FLJ10199 transmembrane protein 51 GeneID:63913 1 4.458 Niban niban protein GeneID:11492 11 4.451 Adam19 M[b] a disintegrin and metallopeptidase domain 19 (meltrin beta) GeneID:56501 X 4.448 Elf4 Mef E74-like factor 4 (ets domain transcription factor) GeneID:380696 11 4.448 LOC380696 similar to heterogeneous nuclear ribonucleoprotein A3 GeneID:76795 11 4.448 2700008N14Rik RP23-319B15.5 RIKEN cDNA 2700008N14 gene GeneID:60321 6 4.443 Wbp11 Npwbp, SIPP1 WW domain binding protein 11 GeneID:627096 6 4.443 LOC627096 hypothetical protein LOC627096 GeneID:320135 6 4.443 C030030A07Rik RIKEN cDNA C030030A07 gene GeneID:16923 5 4.441 Lnk linker of T-cell receptor pathways GeneID:231717 5 4.441 A230106M15Rik RIKEN cDNA A230106M15 gene GeneID:319160 13 4.436 Hist1h4k histone 1, H4k GeneID:319169 13 4.436 Hist1h2ak histone 1, H2ak GeneID:319187 13 4.436 Hist1h2bn histone 1, H2bn GeneID:76108 14 4.435 Rap2a 5830461H18Rik RAS related protein 2a GeneID:239759 16 4.429 Liph Lpdlr, PLA1B, mPA-PLA1 lipase, member H GeneID:625401 16 4.429 LOC625401 hypothetical protein LOC625401 GeneID:56226 4 4.425 Espn GeneID:85030 4 4.425 Tnfrsf25 APO-3, DDR3, DR3, LARD, TR3, TRAMPtumor, necrosisWSL-1, WSL-LR, factor receptor Wsl superfamily, member 25 GeneID:19184 11 4.42 Psmc5 mSUG1 protease (prosome, macropain) 26S subunit, ATPase 5 GeneID:83796 11 4.42 Smarcd2 Baf60b SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 GeneID:75870 11 4.42 Tcam1 testicular cell adhesion molecule 1 GeneID:75089 10 4.418 E030041M21Rik KIAA0701, mKIAA0701 RIKEN cDNA E030041M21 gene GeneID:433254 19 4.417 LOC433254 similar to novel protein GeneID:73713 19 4.417 1110018J23Rik RIKEN cDNA 1110018J23 gene GeneID:207742 11 4.414 4732452J19Rik RP23-449P23.1 RIKEN cDNA 4732452J19 gene GeneID:20922 11 4.414 Supt4h1 Supt4h suppressor of Ty 4 homolog 1 (S. cerevisiae) GeneID:20923 11 4.414 Supt4h2 suppressor of Ty 4 homolog 2 (S. cerevisiae) GeneID:626012 4 4.413 LOC626012 hypothetical protein LOC626012 GeneID:20525 4 4.413 Slc2a1 solute carrier family 2 (facilitated glucose transporter), member 1 GeneID:17281 9 4.404 Fyco1 RUFY3, ZFYVE7 FYVE and coiled-coil domain containing 1 GeneID:80901 9 4.404 Cxcr6 BONZO, BONZO-like, STRL33 chemokine (C-X-C motif) receptor 6 GeneID:22410 15 4.398 Wnt10b wingless related MMTV integration site 10b GeneID:22408 15 4.398 Wnt1 swaying wingless-related MMTV integration site 1 GeneID:223455 15 4.396 6-Mar 3830408G03, mKIAA0597 membrane-associated ring finger (C3HC4) 6 GeneID:320713 4 4.392 Mysm1 -like, SWIRM and MPN domains 1 GeneID:30934 2 4.39 Tor1b DQ1, torsinB torsin family 1, member B GeneID:30931 2 4.39 Tor1a DQ2, MGC18883, torsinA torsin family 1, member A (torsin A) GeneID:97820 13 4.389 4833439L19Rik RIKEN cDNA 4833439L19 gene GeneID:56795 13 4.389 Arl10a Arl10 ADP-ribosylation factor-like 10A GeneID:433294 1 4.387 LOC433294 similar to RIKEN cDNA 4832428D23 gene GeneID:24056 14 4.387 Sh3bp5 Sab SH3-domain binding protein 5 (BTK-associated) GeneID:69780 4 4.384 Smap1l stromal membrane-associated protein 1-like GeneID:245843 16 4.38 4632417D23 hypothetical protein 4632417D23 GeneID:21423 10 4.38 Tcfe2a A1, ALF2, E12, E12/E47, E2A, E47, transcriptionME2, Pan1, Pan2,factor E2aTCF3, VDIR GeneID:74694 10 4.362 4930505D03Rik RIKEN cDNA 4930505D03 gene GeneID:56734 7 4.362 Tulp2 tubby-like protein 2 GeneID:18220 7 4.362 Nucb1 MTEST82, Nucb nucleobindin 1 GeneID:629023 7 4.362 LOC629023 similar to Histone H3.4 (Embryonic) GeneID:546071 8 4.36 LOC546071 similar to KIAA0561 protein GeneID:16161 8 4.36 Il12rb1 CD212, IL-12R[b] interleukin 12 receptor, beta 1 GeneID:70551 14 4.358 4930403J22Rik RIKEN cDNA 4930403J22 gene GeneID:230857 4 4.357 Ece1 ECE-1a, ECE-1b, ECE-1d endothelin converting enzyme 1 GeneID:83430 10 4.354 Il23a p19 interleukin 23, alpha subunit p19 GeneID:103135 10 4.354 Usp52 mKIAA0710 ubiquitin specific peptidase 52 GeneID:67578 2 4.354 4930424G05Rik RIKEN cDNA 4930424G05 gene GeneID:12010 2 4.354 B2m beta2-m beta-2 microglobulin GeneID:70928 2 4.354 Rnf36 Trif, Trimless ring finger protein 36 GeneID:27225 12 4.35 Ddx24 DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 GeneID:52668 12 4.35 D12Ertd647e ISG12a DNA segment, Chr 12, ERATO Doi 647, expressed GeneID:76933 12 4.35 Ifi27 Isg12, Isg12(b1) interferon, alpha-inducible protein 27 GeneID:50908 6 4.349 C1s C1sa, MGC37773 complement component 1, s subcomponent GeneID:623541 6 4.349 LOC623541 hypothetical protein LOC623541 GeneID:58805 5 4.349 Wbscr14 ChREBP, WS-bHLH Williams-Beuren syndrome chromosome region 14 homolog (human) GeneID:11964 16 4.347 Atp6v1a Atp6a2, Atp6v1a1, VA68, VPP2 ATPase, H+ transporting, V1 subunit A1 GeneID:72117 16 4.347 Mak3 Mak3 homolog (S. cerevisiae) GeneID:51897 2 4.347 D2Ertd391e DNA segment, Chr 2, ERATO Doi 391, expressed GeneID:241547 2 4.347 D230010M03Rik RIKEN cDNA D230010M03 gene GeneID:268448 11 4.341 Phf12 F630045O13, PF1, mKIAA1523 PHD finger protein 12 GeneID:70451 11 4.341 2610209N15Rik RP23-43J1.3 RIKEN cDNA 2610209N15 gene GeneID:74401 2 4.341 4933406J08Rik RIKEN cDNA 4933406J08 gene GeneID:329154 1 4.339 E130014H08Rik A130096K20 RIKEN cDNA E130014H08 gene GeneID:103677 11 4.336 AI317223 MGC7717, RP23-143A14.6 expressed sequence AI317223 GeneID:627835 11 4.336 LOC627835 similar to ribosomal protein S12 GeneID:66072 19 4.333 0610038F07Rik MGC61338 RIKEN cDNA 0610038F07 gene GeneID:269061 19 4.333 5730453I16Rik MGC46982 RIKEN cDNA 5730453I16 gene GeneID:67131 11 4.332 Acbd4 acyl-Coenzyme A binding domain containing 4 GeneID:192231 11 4.332 Hexim1 CLP-1, HIS1 hexamethylene bis-acetamide inducible 1 GeneID:66043 10 4.331 Atp5d ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit GeneID:59090 10 4.331 Midn midnolin GeneID:216164 10 4.331 Dos 9630008F14, R29144/1 downstream of Stk11 GeneID:30046 4 4.33 Zfp292 5730450D02Rik, 5830493J20Rik, Zn-15,zinc finger Zn-16, protein mKIAA0530 292 GeneID:621115 4 4.33 LOC621115 hypothetical protein LOC621115 GeneID:20184 13 4.33 Rxrip110 9430016E08Rik, RIP110 interacting protein 110 GeneID:26919 13 4.33 Zfp346 zinc finger protein 346 GeneID:78334 10 4.328 Cdc2l6 CDK11, mKIAA1028 cell division cycle 2-like 6 (CDK8-like) GeneID:107951 2 4.327 Cdk9 PITALRE cyclin-dependent kinase 9 (CDC2-related kinase) GeneID:27387 2 4.327 Sh2d3c Chat, Shep1 SH2 domain containing 3C GeneID:320333 14 4.325 D830030K20Rik RIKEN cDNA D830030K20 gene GeneID:64009 10 4.324 Syne1 8B, CPG2, MGC62825, Myne1, SYNE-1,synaptic SYNE-1B, nuclear mKIAA1756envelope 1 GeneID:67042 15 4.323 Rabl4 RAB, member of RAS oncogene family-like 4 GeneID:68742 7 4.322 1110032O16Rik RIKEN cDNA 1110032O16 gene GeneID:233877 7 4.322 Kctd13 PDIP1alpha, Poldip1 potassium channel tetramerisation domain containing 13 GeneID:625681 12 4.321 LOC625681 similar to Nesprin-2 (Nuclear envelope spectrin repeat protein 2) (Syne-2) (Synaptic nuclear envelope protein 2) (Nucleus and actin connecting element protein) (NUANCE protein) GeneID:257835 11 4.32 GA_x5J8B7W3UM0-970645-971221 olfactory receptor GA_x5J8B7W3UM0-970645-971221 GeneID:257886 11 4.32 Olfr225 MOR256-51 olfactory receptor 225 GeneID:624708 4 4.318 LOC624708 hypothetical protein LOC624708 GeneID:14105 4 4.318 Fusip1 FUSIP2, NSSR1, NSSR2, SRrp40, TFUSASR, interacting TASR1, T ASR2protein (serine-arginine rich) 1 GeneID:431706 13 4.317 Zfp457 Rslcan-6 zinc finger protein 457 GeneID:68731 18 4.312 1110032A13Rik RIKEN cDNA 1110032A13 gene GeneID:27366 18 4.312 Txnl4 Dim1, U5-15kD, U5-15kDa thioredoxin-like 4 GeneID:28062 18 4.312 D18Wsu98e DNA segment, Chr 18, Wayne State University 98, expressed GeneID:77128 17 4.312 A930001N09Rik RIKEN cDNA A930001N09 gene GeneID:208606 5 4.312 1500011J06Rik RIKEN cDNA 1500011J06 gene GeneID:208628 5 4.312 Kntc1 D330012D13 kinetochore associated 1 GeneID:56697 11 4.312 Akap10 D-AKAP2 A kinase (PRKA) anchor protein 10 GeneID:170458 19 4.31 Gpha2 GPA2, zsig51 glycoprotein hormone alpha 2 GeneID:225849 19 4.31 Ppp2r5b protein phosphatase 2, regulatory subunit B (B56), beta isoform GeneID:329015 19 4.31 BC023754 1810013C15Rik, A830054M12, MGC67447,cDNA sequence mKIAA0404 BC023754 GeneID:67201 11 4.309 2700085E05Rik RP23-147P4.6 RIKEN cDNA 2700085E05 gene GeneID:67390 11 4.309 Rnmtl1 HC90, RP23-147P4.7 RNA methyltransferase like 1 GeneID:71952 12 4.309 2410016O06Rik NO66 RIKEN cDNA 2410016O06 gene GeneID:229700 3 4.307 Rbm15 mKIAA1438 RNA binding motif protein 15 GeneID:383901 3 4.307 LOC383901 similar to 14-3-3 protein sigma GeneID:68988 7 4.305 Prpf31 PRP31, RP11 PRP31 pre-mRNA processing factor 31 homolog (yeast) GeneID:208777 1 4.303 Sned1 SST3, Snep sushi, nidogen and EGF-like domains 1 GeneID:69821 1 4.303 Mterfd2 MTERF domain containing 2 GeneID:269224 1 4.303 Pask mKIAA0135 PAS domain containing serine/threonine kinase GeneID:76498 17 4.302 Paqr4 progestin and adipoQ receptor family member IV GeneID:73016 17 4.302 Kremen2 kringle containing transmembrane protein 2 GeneID:224613 17 4.302 Flywch1 E030034P13Rik, mKIAA1552 FLYWCH-type zinc finger 1 GeneID:51793 17 4.299 Ddah2 1110003M04Rik, Ddah, G6a, NG30 dimethylarginine dimethylaminohydrolase 2 GeneID:19652 X 4.29 Rbm3 RNA binding motif protein 3 GeneID:209815 X 4.29 Oatl1 A530047E07, mMg81 ornithine aminotransferase-like 1 GeneID:11747 3 4.289 Anxa5 Anx5 GeneID:22029 2 4.287 Traf1 Tnf receptor-associated factor 1 GeneID:66349 7 4.287 2310004L02Rik RIKEN cDNA 2310004L02 gene GeneID:232984 7 4.287 B3galt7 B7galt7, MGC32391 UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 7 GeneID:22375 12 4.285 Wars WRS tryptophanyl-tRNA synthetase GeneID:213068 15 4.282 Tmem71 E430024D12 ransmembrane protein 71 GeneID:624085 15 4.282 LOC624085 similar to PHD finger protein 20-like 1 GeneID:619719 3 4.282 LOC619719 hypothetical protein LOC619719 GeneID:269423 3 4.282 3110057O12Rik 4732404H23 RIKEN cDNA 3110057O12 gene GeneID:73297 3 4.282 1700034I23Rik RIKEN cDNA 1700034I23 gene GeneID:21814 5 4.281 Tgfbr3 TBRIII transforming growth factor, beta receptor III GeneID:252966 2 4.278 Cables2 ik3-2 Cdk5 and Abl enzyme substrate 2 GeneID:271887 2 4.278 4732416F18 hypothetical protein 4732416F18 GeneID:13595 X 4.275 Ebp Pabp, mSI phenylalkylamine Ca2+ antagonist (emopamil) binding protein GeneID:53627 X 4.275 Porcn Mporc, Mporc-a, Mporc-b, Mporc-c, Mporc-d,porcupine Ppn, homolog mMg61, (Drosophila) porc GeneID:26450 2 4.274 Rbbp9 Bog retinoblastoma binding protein 9 GeneID:52065 8 4.274 Mfhas1 malignant fibrous histiocytoma amplified sequence 1 GeneID:18173 1 4.274 Slc11a1 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 GeneID:227292 1 4.274 Ctdsp1 GIP, NLIIF, Nif3, SCP1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 GeneID:319161 13 4.273 Hist1h4m histone 1, H4m GeneID:319171 13 4.273 Hist1h2ao histone 1, H2ao GeneID:54563 6 4.27 Nup210 9830001L10, gp190, gp210 nucleoporin 210 GeneID:28080 16 4.263 Atp5o ATPO, OSCP ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit GeneID:15968 4 4.261 Ifna5 interferon alpha family, gene 5 GeneID:15967 4 4.261 Ifna4 interferon alpha family, gene 4 GeneID:15962 4 4.261 Ifna1 interferon alpha family, gene 1 GeneID:68808 13 4.26 1110046J04Rik RIKEN cDNA 1110046J04 gene GeneID:18105 13 4.26 Nqo2 NMO2 NAD(P)H dehydrogenase, quinone 2 GeneID:71690 13 4.259 Esm1 ESM-1 endothelial cell-specific molecule 1 GeneID:80281 3 4.257 Cttnbp2nl MGC7720, mKIAA1433 CTTNBP2 N-terminal like GeneID:75269 3 4.257 4930564D02Rik RIKEN cDNA 4930564D02 gene GeneID:230983 4 4.256 Pex10 peroxisome biogenesis factor 10 GeneID:67830 4 4.256 Rer1 RER1 retention in 1 homolog (S. cerevisiae) GeneID:76866 4 4.256 4930417P05Rik RIKEN cDNA 4930417P05 gene GeneID:224671 17 4.253 Btbd9 4930402L05 BTB (POZ) domain containing 9 GeneID:12021 1 4.251 Bard1 BRCA1 associated RING domain 1 GeneID:20893 6 4.248 Bhlhb2 CR8, Clast5, Dec1, Sharp2, Stra13 basic helix-loop-helix domain containing, class B2 GeneID:27015 13 4.245 Polk polymerase (DNA directed), kappa GeneID:209387 7 4.242 AI451617 expressed sequence AI451617 GeneID:320207 11 4.239 Pik3r5 RP23-432M23.2 phosphoinositide-3-kinase, regulatory subunit 5, p101 GeneID:71776 11 4.238 Tha1 GLY1 threonine aldolase 1 GeneID:546519 11 4.238 LOC546519 hypothetical protein LOC546519 GeneID:226652 1 4.237 Arhgap30 mFLJ00267 Rho GTPase activating protein 30 GeneID:22278 1 4.237 Usf1 upstream transcription factor 1 GeneID:226654 1 4.237 LOC226654 similar to KAT protein GeneID:545622 4 4.234 LOC545622 similar to Tyrosine-protein phosphatase, non-receptor type 3 (Protein-tyrosine phosphatase H1) (PTP-H1) GeneID:67441 7 4.232 0610042E07Rik Isoc2 RIKEN cDNA 0610042E07 gene GeneID:114565 16 4.229 Zfp295 Znf295, mKIAA1227 zinc finger protein 295 GeneID:56551 15 4.225 Txn2 thioredoxin 2 GeneID:625042 1 4.225 LOC625042 hypothetical protein LOC625042 GeneID:68226 1 4.225 Efcab2 EF-hand calcium binding domain 2 GeneID:57915 5 4.212 Tbc1d1 Tbc1, mKIAA1108 TBC1 domain family, member 1 GeneID:66540 2 4.203 3110001A13Rik RIKEN cDNA 3110001A13 gene GeneID:622055 2 4.2 LOC622055 hypothetical protein LOC622055 GeneID:73137 18 4.198 1190002C06Rik RIKEN cDNA 1190002C06 gene GeneID:329509 2 4.192 1810024B03Rik RIKEN cDNA 1810024B03 gene GeneID:320632 2 4.192 Ascc3l1 HELIC2, KIAA0788, U5-200-KD, U5-200KDactivating signal cointegrator 1 complex subunit 3-like 1 GeneID:338363 18 4.189 6030446N20Rik RIKEN cDNA 6030446N20 gene GeneID:66878 18 4.189 Riok3 RIO kinase 3 (yeast) GeneID:69550 8 4.188 Bst2 DAMP-1 bone marrow stromal cell antigen 2 GeneID:73711 8 4.188 1110012M11Rik RIKEN cDNA 1110012M11 gene GeneID:22021 5 4.187 Tpst1 protein-tyrosine sulfotransferase 1 GeneID:20535 5 4.186 Slc4a2 B3RP solute carrier family 4 (anion exchanger), member 2 GeneID:66587 5 4.186 Fastk 610011K02Rik Fas-activated serine/threonine kinase GeneID:64295 5 4.186 2010004O20Rik Hops RIKEN cDNA 2010004O20 gene GeneID:213990 5 4.186 Centg3 MGC37541, MRIP-1 centaurin, gamma 3 GeneID:65098 7 4.186 Za20d3 Awp1 zinc finger, A20 domain containing 3 GeneID:106369 16 4.184 Ypel1 Dgl1, mdgl-1 yippee-like 1 (Drosophila) GeneID:66053 16 4.184 Ppil2 0610009L05Rik, 1700016N17Rik, 4921520K19Rik,peptidylprolyl isomerase 4930511F14Rik (cyclophilin)-like 2 GeneID:545015 14 4.181 2610042L04Rik RIKEN cDNA 2610042L04 gene GeneID:30841 5 4.173 Fbxl10 Cxxc2, E430001G17, Fbl10, KIAA3014,F-box PCCX2, and leucine-rich mKIAA3014 repeat protein 10 GeneID:109202 5 4.173 A930024E05Rik RIKEN cDNA A930024E05 gene GeneID:66923 14 4.173 2610016F04Rik BAF180, Pb1 RIKEN cDNA 2610016F04 gene GeneID:66487 14 4.173 2010107H07Rik RIKEN cDNA 2010107H07 gene GeneID:223646 15 4.173 Naprt1 nicotinate phosphoribosyltransferase domain containing 1 GeneID:66656 15 4.173 Eef1d eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) GeneID:56463 6 4.172 AL033314 Snd1 expressed sequence AL033314 GeneID:320040 11 4.168 9930039A11Rik RP23-396M19.3 RIKEN cDNA 9930039A11 gene GeneID:629029 7 4.168 LOC629029 similar to Sulfotransferase 1A1 (Aryl sulfotransferase) (Phenol sulfotransferase) (Sulfokinase) (Phenol/aryl sulfotransferase) (mSTp1) (ST1A4) GeneID:75764 7 4.168 Giyd2 GIY-YIG domain containing 2 GeneID:66162 7 4.168 Bola2 BolA-2 bolA-like 2 (E. coli) GeneID:12721 7 4.168 Coro1a TACO, p57 coronin, actin binding protein 1A GeneID:17937 10 4.167 Nab2 Ngfi-A binding protein 2 GeneID:210035 10 4.167 BC030440 MGC40914 cDNA sequence BC030440 GeneID:20852 10 4.167 Stat6 signal transducer and activator of transcription 6 GeneID:83431 11 4.166 Ndel1 MITAP1, NUDEL nuclear distribution gene E-like homolog 1 (A. nidulans) GeneID:71728 1 4.158 Stk11ip LIP1, LKB1IP serine/threonine kinase 11 interacting protein GeneID:13723 13 4.157 Emb MGC21425 embigin GeneID:19332 8 4.155 Rab20 RAB20, member RAS oncogene family GeneID:98828 2 4.153 G431001I09Rik RIKEN cDNA G431001I09 gene GeneID:53893 2 4.153 Nudt5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 GeneID:237433 10 4.153 LOC237433 similar to ribosomal protein S12 GeneID:104303 10 4.153 Arl1 ADP-ribosylation factor-like 1 GeneID:18582 1 4.152 Pde6d phosphodiesterase 6D, cGMP-specific, rod, delta GeneID:26895 1 4.152 Cops7b E130114M23 COP9 (constitutive photomorphogenic) homolog, subunit 7b (Arabidopsis thaliana) GeneID:22027 10 4.152 Tra1 ERp99, GRP94, gp96 tumor rejection antigen gp96 GeneID:103220 10 4.152 Gnn MGC40847 Grp94 neighboring nucleotidase variant 4 GeneID:12352 8 4.149 Car5a CAV carbonic anhydrase 5a, mitochondrial GeneID:53325 8 4.149 Banp SMAR1 Btg3 associated GeneID:227627 2 4.148 Lcn13 MGC41397 lipocalin 13 GeneID:107771 2 4.148 Bmyc brain expressed myelocytomatosis oncogene GeneID:332578 2 4.148 Lcn10 lipocalin 10 GeneID:269282 2 4.143 Myo3b myosin IIIB GeneID:13006 19 4.143 Cspg6 Bamacan, HCAP, SMC3, SmcD chondroitin sulfate proteoglycan 6 GeneID:66625 4 4.134 5730406M06Rik RIKEN cDNA 5730406M06 gene GeneID:230027 4 4.134 Coq3 4732433J24 coenzyme Q3 homolog, methyltransferase (yeast) GeneID:226641 1 4.127 Atf6 9630036G24 activating transcription factor 6 GeneID:80915 1 4.127 Dusp12 LMW-DSP4, T-DSP4, VH1, mVH1 dual specificity phosphatase 12 GeneID:74471 6 4.126 4933440N22Rik RIKEN cDNA 4933440N22 gene GeneID:98758 6 4.126 Hnrpf MGC36971 heterogeneous nuclear ribonucleoprotein F GeneID:170752 9 4.125 Bcdo2 B-diox-II beta-carotene 9', 10'-dioxygenase 2 GeneID:66654 9 4.125 Tex12 testis expressed gene 12 GeneID:78809 16 4.123 4930562C15Rik RIKEN cDNA 4930562C15 gene GeneID:19286 9 4.12 Pts PTPS 6-pyruvoyl-tetrahydropterin synthase GeneID:74717 1 4.119 4930513F16Rik MSRG-11 RIKEN cDNA 4930513F16 gene GeneID:67769 1 4.119 Gpatc2 G patch domain containing 2 GeneID:623164 8 4.114 LOC623164 similar to zinc finger protein 177 GeneID:623183 8 4.114 LOC623183 similar to zinc finger protein 97 GeneID:623223 8 4.114 LOC623223 hypothetical protein LOC623223 GeneID:232906 7 4.114 6430596G11Rik mKIAA1722 RIKEN cDNA 6430596G11 gene GeneID:620871 3 4.113 LOC620871 similar to Cyclin-dependent regulatory subunit 1 (CKS-1) (Sid 1334) GeneID:216152 10 4.111 BC005764 Prg2, mKIAA4076 cDNA sequence BC005764 GeneID:19152 10 4.111 Prtn3 mPR3 proteinase 3 GeneID:50701 10 4.111 NE NE elastase GeneID:75387 5 4.111 Sirt4 4 (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) GeneID:227612 2 4.109 A830007P12Rik MGC7940 RIKEN cDNA A830007P12 gene GeneID:58202 2 4.109 A730008L03Rik Cobra1, Nelf-b RIKEN cDNA A730008L03 gene GeneID:620897 8 4.109 LOC620897 similar to Ubiquitin-conjugating enzyme E2 E3 (Ubiquitin-protein ligase E3) (Ubiquitin carrier protein E3) (Ubiquitin-conjugating enzyme E2-23 kDa) (UbcM2) GeneID:20399 7 4.108 Sh2bpsm1 SH2-B, SH2-Bb, mKIAA1299 SH2-B PH domain containing signaling mediator 1 GeneID:13197 6 4.105 Gadd45a GADD45 growth arrest and DNA-damage-inducible 45 alpha GeneID:27403 10 4.104 Abca7 ABCX, Abc51 ATP-binding cassette, sub-family A (ABC1), member 7 GeneID:70719 10 4.104 6330406L22Rik Ha-1 RIKEN cDNA 6330406L22 gene GeneID:93691 1 4.104 Klf7 Kruppel-like factor 7 (ubiquitous) GeneID:114715 2 4.104 Spred1 sprouty protein with EVH-1 domain 1, related sequence GeneID:386649 2 4.103 Nsfl1c MGC61403, MGC86097, Munc-18c, NSFL1Stxbp3a, (p97) p47 cofactor (p47) GeneID:66161 7 4.099 Pop4 Rpp29 processing of precursor 4, ribonuclease P/MRP family, (S. cerevisiae) GeneID:57316 11 4.097 C1d SUN-CoR nuclear DNA binding protein GeneID:13435 12 4.094 Dnmt3a DNA methyltransferase 3A GeneID:17999 9 4.094 Nedd4 Nedd4-1, mKIAA0093 neural precursor cell expressed, developmentally down-regulted gene 4 GeneID:317677 6 4.093 C1sb complement component C1SB GeneID:108017 6 4.093 Fxyd4 Chif FXYD domain-containing ion transport regulator 4 GeneID:14718 19 4.093 Got1 cAspAT glutamate oxaloacetate transaminase 1, soluble GeneID:20308 11 4.089 Ccl9 CCF18, MRP-2 chemokine (C-C motif) ligand 9 GeneID:414102 11 4.089 E230016K23Rik RIKEN cDNA E230016K23 gene GeneID:20305 11 4.089 Ccl6 MRP-1, c10 chemokine (C-C motif) ligand 6 GeneID:13175 3 4.088 Dcamkl1 Cpg16, DCLK, mKIAA0369 double cortin and calcium/calmodulin-dependent protein kinase-like 1 GeneID:54354 1 4.088 Rassf5 Maxp1, Nore1, Nore1A, Nore1B, RaplRas association (RalGDS/AF-6) domain family 5 GeneID:628074 7 4.087 LOC628074 hypothetical protein LOC628074 GeneID:20679 7 4.087 Sox6 SRY-box containing gene 6 GeneID:75965 14 4.083 Zdhhc20 zinc finger, DHHC domain containing 20 GeneID:70497 7 4.083 Arhgap17 Nadrin, Rich1, Wbp15 Rho GTPase activating protein 17 GeneID:30949 7 4.083 Lcmt1 MGC11807 leucine carboxyl methyltransferase 1 GeneID:229715 3 4.076 Amigo1 A, Amigo, MGC25558, ali2 adhesion molecule with Ig like domain 1 GeneID:72023 3 4.076 Cyb561d1 cytochrome b-561 domain containing 1 GeneID:72522 3 4.076 Atxn7l2 ataxin 7-like 2 GeneID:243025 5 4.075 Gm446 gene model 446, (NCBI) GeneID:68219 8 4.074 Nudt21 25kDa nudix (nucleoside diphosphate linked moiety X)-type motif 21 GeneID:270086 8 4.074 4930415J21Rik mKIAA1612 RIKEN cDNA 4930415J21 gene GeneID:226412 1 4.074 R3hdm1 R3hdm R3H domain 1 (binds single-stranded nucleic acids) GeneID:67812 1 4.074 Ubxd2 mKIAA0242 UBX domain containing 2 GeneID:20698 11 4.07 Sphk1 SK1 sphingosine kinase 1 GeneID:217342 11 4.07 B230113M03Rik 9630022H21, E2-230K, mKIAA1734RIKEN cDNA B230113M03 gene GeneID:236915 X 4.068 Arhgef9 A230067K14, mKIAA0424 Cdc42 guanine nucleotide exchange factor (GEF) 9 GeneID:72119 2 4.068 Tpx2 DIL2, REPP86, p100 TPX2, microtubule-associated protein homolog (Xenopus laevis) GeneID:71223 16 4.065 Gpr15 G protein-coupled receptor 15 GeneID:224250 16 4.065 1110019C08Rik RIKEN cDNA 1110019C08 gene GeneID:78617 2 4.065 Cstad CSA-conditional, T cell activation-dependent protein GeneID:226922 1 4.063 Kcnq5 7730402H11 potassium voltage-gated channel, subfamily Q, member 5 GeneID:319615 17 4.062 6330416L07Rik RIKEN cDNA 6330416L07 gene GeneID:224598 17 4.062 BC021442 MGC29357, MGC67807 cDNA sequence BC021442 GeneID:20439 3 4.061 Siah2 seven in absentia 2 GeneID:623388 3 4.061 LOC623388 hypothetical protein LOC623388 GeneID:59052 7 4.056 0610012D09Rik Drev, MNCb-5680 RIKEN cDNA 0610012D09 gene GeneID:15926 1 4.055 Idh1 IDPc isocitrate dehydrogenase 1 (NADP+), soluble GeneID:18711 1 4.055 Pip5k3 Pip5k, Pipk5k3, p235 phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III GeneID:19134 13 4.053 Prpf4b 2610037H07, MGC5960, Prp4, Prp4k,PRP4 cbp143 pre-mRNA processing factor 4 homolog B (yeast) GeneID:433948 5 4.052 LOC433948 hypothetical LOC433948 GeneID:109305 5 4.052 D730049H07Rik RIKEN cDNA D730049H07 gene GeneID:30940 16 4.051 Usp25 ubiquitin specific peptidase 25 GeneID:93675 6 4.048 Clec2i Clr-g, Clrg, LCL-1, OCILrP2 C-type lectin domain family 2, member i GeneID:28042 5 4.046 D5Wsu178e KIAA1724, SELI, mKIAA1724 DNA segment, Chr 5, Wayne State University 178, expressed GeneID:14056 6 4.044 Ezh2 Enx-1, mKIAA4065 enhancer of zeste homolog 2 (Drosophila) GeneID:66336 13 4.042 1700022C02Rik RIKEN cDNA 1700022C02 gene GeneID:27047 13 4.042 Omd SLRR2C osteomodulin GeneID:13002 2 4.041 Dnajc5 DnaJ (Hsp40) homolog, subfamily C, member 5 GeneID:66441 6 4.038 2010012C16Rik mago, magoh RIKEN cDNA 2010012C16 gene GeneID:243659 6 4.038 Styk1 9130025L13, Nok serine/threonine/tyrosine kinase 1 GeneID:106021 4 4.037 Topors LUN, TP53BPL, p53BP3/LUN topoisomerase I binding, arginine/serine-rich GeneID:230075 4 4.037 Gm137 gene model 137, (NCBI) GeneID:20556 11 4.035 Slfn2 Shlf2 schlafen 2 GeneID:384856 8 4.031 LOC384856 similar to GeneID:81489 8 4.031 Dnajb1 HSPF1, Hsp40 DnaJ (Hsp40) homolog, subfamily B, member 1 GeneID:50776 11 4.028 Polg2 polymerase (DNA directed), gamma 2, accessory subunit GeneID:13207 11 4.028 Ddx5 G17P1, HUMP68, p68 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 GeneID:320162 11 4.028 4732496G21Rik D12Mgi16, D330027A14Rik RIKEN cDNA 4732496G21 gene GeneID:13714 1 4.023 Elk4 Sap1 ELK4, member of ETS oncogene family GeneID:59125 1 4.023 Nek7 NIMA (never in mitosis gene a)-related expressed kinase 7 GeneID:226243 19 4.021 Habp2 HABP, HGFAL, MGC28705, PHBP hyaluronic acid binding protein 2 GeneID:80782 6 4.016 Klrb1d NKR-P1D, Nkrp1d killer cell lectin-like receptor subfamily B member 1D GeneID:17058 6 4.016 Klrb1b NKR-P1 34, NKR-P1B, Nkrp1-b killer cell lectin-like receptor subfamily B member 1B GeneID:11767 8 4.015 Ap1m1 AP47, Adtm1A, [m]1A, mu1A adaptor-related protein complex AP-1, mu subunit 1 GeneID:27392 1 4.014 Pign phosphatidylinositol glycan, class N GeneID:227446 1 4.014 2310035C23Rik 6430401N10, mKIAA1468 RIKEN cDNA 2310035C23 gene GeneID:230793 4 4.007 Ahdc1 MGC29331 AT hook, DNA binding motif, containing 1 GeneID:620487 4 4.006 LOC620487 similar to Midasin (MIDAS-containing protein) GeneID:114716 11 4.002 Spred2 sprouty-related, EVH1 domain containing 2 Supplementary Table 2 A. Common B. TCR STAT4 C. Common genes in genes in signaling ChIP-on- testis microarray and microarrays and genes chip STAT4 ChIP-on-chip STAT4-ChIP- target on-chip Il18r1 Cblb YES Arih1 Luc7l Cd3z YES Trp53i5 Plp2 Ikbke YES Crsp7 Serpine1 Lcp2 YES D030041N04Rik Gbp2 Lnk YES Plp2 Igtp Prkcq YES Dgkg Ifi35 Wasl YES Cdc6 Gzmb Zap70 NO 4921520G13Rik Per3 Lck NO Rnaseh2a Sdc4 Malt1 NO Trim27 Pml Itk NO Cyct Ext2 Tcra NO Prm1 Gbp1 Tcrb NO 2410018G20Rik Il12rb2 Cd3e NO Rg9mtd2 Ifng CD3d NO Nkg7 Tnf CD3g NO Gfpt1 Muc1 Cd3eta NO Psmb9 Socs1 Vav NO Actr10 Cxcl10 Carma NO Pvt1 Casp8 Fyn NO Rab27a Ly6c Lat NO Kpnb1 Irf4 Bcl10 NO Gcnt3 Cd69 Fyb NO Ubxd5 Lif Mcm3 Ptger4 Hist1h4a Mllt3 Jmjd3 Entpd4 Stat4 Mcm3 Nsmce1 Rnh1 Akap10 Pdcd4 Eif2ak1 Camta1 Cast Pcgf5 Sema3b Furin Plac8 Lrp8 Prkcq Pold4 Pja2 Tnfsf10 X83328 Ccnd2 Kpna6 Ier3 Ccr4 Pank2 D16Ertd472e Dap Irf8 Ctsw 1700017D01Rik Ifi47 Clic1 Plac8 Aif1 Pscdbp Nbr1 Nfil3 4930534B04Rik Lag3 Zbp1 Irf8 Ppp2r5e Ahnak Tor1aip1 Adarb1 Zbtb32 Vamp3 Zpbp2 Fvt1 Cd52 Znrf1 Pdzk1ip1 Zbtb32 Anxa13 Gadd45g Ltb Prr13 Smpdl3b Birc3 Lpxn Abtb2 Cdh17 Irf1 Tbc1d14 Ltb Frag1 Osm Trim17 Per1 Cubn Lpxn Tcam1 Cebpd Mobkl2c Ly6a Prm2 Stat1 Ppp3cc Mtrf1 Klrc1 Tgtp C730024G19Rik Mesdc2 Mapkapk3 Tap1 Tnp2 Irf7 Gkap1 Pak2 Tns1 Cpd 1700040L02Rik D11Wsu99e Nusap1 Wdfy1 Tes Samhd1 Adora3 Tex12 Slc7a7 Amhr2 Muc1 Nt5e Wdr48 Cpt1b Ptger4 Ms4a4b Tex15 Gm603 Pcgf5 Asxl1 Gprk2l Ddx19b D11Wsu47e Slamf1 Ahi1 Treml2 Cdc20 Pscdbp Efcab2 Rbl1 Emp1 1700025E21Rik 2610528E23Rik Creb1 Dgat1 Sec23ip Ccr7 BC030440 Fbxo30 Galnt3 Cetn4 Kcnn4

Supplementary Table 3

Gene Protein STAT4 ChIP-on- Stat4-dependent T-bet-dependent chip target expression in Th1 expression in Th1 (citation) (citation) Ifng IFNγ Yes Yes (1, 2) Yes (3) Lta Lymphotoxin-α Yes Yes (4) Yes(4) Ltb Lymphotoxin-β Yes ? ? Tnf Tumor necrosis Yes Yes (5) ? factor-α Xcl1 Lymphotactin No No (6) Yes (4) Osm Oncostatin M Yes Yes (7) ? Hlx1 Hlx No Yes (4) Yes Etv5 ERM No Yes (8) Yes (4) Egr2 Egr2 Yes No (4) Yes (4) Egr3 Egr3 No No (4) Yes (4) Irf1 IRF-1 Yes Yes (9) ? Twist1 Twist1 No Yes (10) No (10) Runx3 Runx3 Yes Yes (4) Yes (11) Il12rb1 IL-12Rβ1 Yes Yes (12) ? Il12rb2 IL-12Rβ2 Yes Yes (12) Yes (13) Il18r1 IL-18Rα Yes Yes (12) Yes (4) Il18rap IL-18Rβ Yes Yes (14) Yes (4) Ccr5 CCR5 No Yes (15) Yes (4, 16) Cxcr3 CXCR3 No No (17) Yes (16) Fut7 FucTVII No No (18) Yes (19) St3gal6 ST3GalVI No Yes (19) Yes (19) Gcnt1 C2GlcNacT No Yes (18) Yes (19) Furin Furin Yes Yes (20) No (4) Gadd45b GADD45β Yes Yes (this report) ? Gadd45g GADD45γ Yes Yes (this report) ? Map3k8 Tpl2 Yes Yes (21) ? Ms4a4b Tetraspanin Yes No (22) ? 4a4b Myd88 MyD88 Yes Yes (this report) ?

References 1. Kaplan, M. H., Y.-L. Sun, T. Hoey, and M. J. Grusby. 1996. Impaired IL-12 responses and enhanced development of Th2 cells in Stat4-deficient mice. Nature 382:174-177. 2. Thierfelder, W. E., J. M. van Deursen, K. Yamamoto, R. A. Tripp, S. R. Sarawar, R. T. Carson, M. Y. Sangster, D. A. A. Vignali, P. C. Doherty, G. C. Grosveld, and J. N. Ihle. 1996. Requirement for Stat4 in interleukin-12 mediated responses of natural killer and T cells. Nature 382:171-174. 3. Szabo, S. J., B. M. Sullivan, C. Stemmann, A. R. Satoskar, B. P. Sleckman, and L. H. Glimcher. 2002. Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science 295:338-342. 4. Thieu, V. T., Q. Yu, H. C. Chang, N. Yeh, E. T. Nguyen, S. Sehra, and M. H. Kaplan. 2008. Signal transducer and activator of transcription 4 is required for the transcription factor T-bet to promote T helper 1 cell-fate determination. Immunity 29:679-690. 5. O'Malley, J. T., R. D. Eri, G. L. Stritesky, A. N. Mathur, H. C. Chang, H. HogenEsch, M. S. Srinivasan, and M. H. Kaplan. 2008. STAT4 isoforms differentially regulate Th1 cytokine production and the severity of inflammatory bowel disease. J. Immunol. 181:5062-5070. 6. Zhang, S., N. W. Lukacs, V. A. Lawless, S. L. Kunkel, and M. H. Kaplan. 2000. Differential expression of chemokines in Th1 and Th2 cells is dependent on Stat6 but not Stat4. J. Immunol. 165:10-14. 7. Broxmeyer, H. E., H. A. Bruns, S. Zhang, S. Cooper, G. Hangoc, A. N. J. McKenzie, A. L. Dent, U. Schindler, L. K. Naeger, T. Hoey, and M. H. Kaplan. 2002. Th1 cells regulate hematopoietic progenitor cell homeostasis by production of Oncostatin M. Immunity 16:815-825. 8. Ouyang, W., N. G. Jacobson, D. Bhattacharya, J. D. Gorham, D. Fenoglio, W. C. Sha, T. L. Murphy, and K. M. Murphy. 1999. The Ets transcription factor ERM is Th1-specific and induced by IL-12 through a Stat4-dependent pathway. Proc Natl Acad Sci U S A 96:3888-3893. 9. Hoey, T., S. Zhang, N. Schmidt, Q. Yu, S. Ramchandani, X. Xu, L. K. Naeger, Y. L. Sun, and M. H. Kaplan. 2003. Distinct requirements for the naturally occurring splice forms Stat4α and Stat4β in IL-12 responses. EMBO J 22:4237-4248. 10. Niesner, U., I. Albrecht, M. Janke, C. Doebis, C. Loddenkemper, M. H. Lexberg, K. Eulenburg, S. Kreher, J. Koeck, R. Baumgrass, K. Bonhagen, T. Kamradt, P. Enghard, J. Y. Humrich, S. Rutz, U. Schulze-Topphoff, O. Aktas, S. Bartfeld, H. Radbruch, A. N. Hegazy, M. Lohning, D. C. Baumgart, R. Duchmann, M. Rudwaleit, T. Haupl, I. Gitelman, V. Krenn, J. Gruen, J. Sieper, M. Zeitz, B. Wiedenmann, F. Zipp, A. Hamann, M. Janitz, A. Scheffold, G. R. Burmester, H. D. Chang, and A. Radbruch. 2008. Autoregulation of Th1-mediated inflammation by twist1. J Exp Med 205:1889-1901. 11. Djuretic, I. M., D. Levanon, V. Negreanu, Y. Groner, A. Rao, and K. M. Ansel. 2007. Transcription factors T-bet and Runx3 cooperate to activate Ifng and silence Il4 in T helper type 1 cells. Nat Immunol 8:145-153. 12. Lawless, V. A., S. Zhang, O. N. Ozes, H. A. Bruns, I. Oldham, T. Hoey, M. J. Grusby, and M. H. Kaplan. 2000. Stat4 regulates multiple components of IFN-γ- inducing signaling pathways. J. Immunol. 165:6803-6808. 13. Usui, T., J. C. Preiss, Y. Kanno, Z. J. Yao, J. H. Bream, J. J. O'Shea, and W. Strober. 2006. T-bet regulates Th1 responses through essential effects on GATA- 3 function rather than on IFNG gene acetylation and transcription. J Exp Med 203:755-766. 14. Nakahira, M., M. Tomura, M. Iwasaki, H.-J. Ahn, Y. Blan, T. Hamaoka, T. Ohta, M. Kurimoto, and H. Fujiwara. 2001. An absolute requirement for Stat4 and a role for IFN-γ as an amplifying factor in IL-12 induction of the functional IL-18 receptor complex. J. Immunol. 167:1306-1312. 15. Iwasaki, M., T. Mukai, C. Nakajima, Y. F. Yang, P. Gao, N. Yamaguchi, M. Tomura, H. Fujiwara, and T. Hamaoka. 2001. A mandatory role for STAT4 in IL- 12 induction of mouse T cell CCR5. J Immunol 167:6877-6883. 16. Lord, G. M., R. M. Rao, H. Choe, B. M. Sullivan, A. H. Lichtman, F. W. Luscinskas, and L. H. Glimcher. 2005. T-bet is required for optimal proinflammatory CD4+ T-cell trafficking. Blood 106:3432-3439. 17. Nakajima, C., T. Mukai, N. Yamaguchi, Y. Morimoto, W. R. Park, M. Iwasaki, P. Gao, S. Ono, H. Fujiwara, and T. Hamaoka. 2002. Induction of the chemokine receptor CXCR3 on TCR-stimulated T cells: dependence on the release from persistent TCR-triggering and requirement for IFN-gamma stimulation. Eur J Immunol 32:1792-1801. 18. White, S. J., G. H. Underhill, M. H. Kaplan, and G. S. Kansas. 2001. Cutting edge: differential requirements for Stat4 in expression of responsible for ligand formation in Th1 cells. J Immunol 167:628-631. 19. Underhill, G. H., D. G. Zisoulis, K. P. Kolli, L. G. Ellies, J. D. Marth, and G. S. Kansas. 2005. A crucial role for T-bet in selectin ligand expression in T helper 1 (Th1) cells. Blood 106:3867-3873. 20. Pesu, M., L. Muul, Y. Kanno, and J. J. O'Shea. 2006. Proprotein convertase furin is preferentially expressed in T helper 1 cells and regulates interferon gamma. Blood 108:983-985. 21. Watford, W. T., B. D. Hissong, L. R. Durant, H. Yamane, L. M. Muul, Y. Kanno, C. M. Tato, H. L. Ramos, A. E. Berger, L. Mielke, M. Pesu, B. Solomon, D. M. Frucht, W. E. Paul, A. Sher, D. Jankovic, P. N. Tsichlis, and J. J. O'Shea. 2008. Tpl2 kinase regulates T cell interferon-gamma production and host resistance to Toxoplasma gondii. J Exp Med 205:2803-2812. 22. Venkataraman, C., G. Schaefer, and U. Schindler. 2000. Cutting edge: Chandra, a novel four-transmembrane domain protein differentially expressed in helper type 1 . J Immunol 165:632-636.