St. Marys River Monitoring Projects for TMDL Development
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FINAL REPORT ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT PREPARED FOR: MICHIGAN DEPARTMENT OF NATURAL RESOURCES AND ENVIRONMENT 525 WEST ALLEGAN STREET, PO BOX 30473 LANSING, MI 48909-7973 U.S. ENVIRONMENTAL PROTECTION AGENCY REGION 5 77 WEST JACKSON BOULEVARD CHICAGO, IL 60604-3507 USEPA CONTRACT NUMBER EP-RS-10-03 TASK ORDER 0001 DECEMBER, 2010 ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT FINAL REPORT Citation Format: Montgomery Associates (2010). St. Marys River Monitoring Project for TMDL Development: Final Report. Prepared for U.S. Environmental Protection Agency Region 5 and Michigan Department of Natural Resources and Environment. ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT FINAL REPORT TABLE OF CONTENTS 1. PROJECT SUMMARY................................................................................................................................1 1.1. Project Purpose.....................................................................................................................................1 1.2. Background ..........................................................................................................................................1 1.3. Project Team Organization...................................................................................................................2 2. METHODS...................................................................................................................................................6 2.1 Sampling Overview..............................................................................................................................6 2.2 Sampling LocationS .............................................................................................................................6 2.3 SAMPLing METHODS .......................................................................................................................6 2.4 Laboratory Analytical Methods..........................................................................................................10 3. SAMPLING RESULTS .............................................................................................................................10 3.1 E. coli analysis ...................................................................................................................................10 3.2 BST analysis.......................................................................................................................................12 3.3 Precipitation .......................................................................................................................................14 4. QUALITY ASSURANCE SUMMARY ....................................................................................................14 5. SOURCE IDENTIFICATION ...................................................................................................................15 6. REFERENCES...........................................................................................................................................17 TABLES Table 1: St. Marys River impaired segments on Michigan’s approved 2008 303(d) List .........................2 Table 2: Key project team members................................................................................................................3 Table 3: Sampling locations ..............................................................................................................................8 Table 4: Sampling schedule ..............................................................................................................................9 Table 5: Analytical methods and details.......................................................................................................10 Table 6: Frequency of exceedance of MDNR total body contact WQSs (daily maximum of 300 E. coli per 100mL and 30-day geometric mean of 130 E. coli per 100mL) and partial body contact WQS (daily maximum of 1,000 E. coli per 100mL). ...............................................................................................11 Table 7: Bacterial source tracking results......................................................................................................13 Table 8: Summary of field and laboratory completeness for E. coli samples...........................................15 ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT FINAL REPORT PAGE i FIGURES Figure 1: St. Marys River transect sampling locations............................................................................... 19 Figure 2: Tributary sampling locations........................................................................................................ 20 Figure 3: Week 1 E. coli results...................................................................................................................... 21 Figure 4: Week 2 E. coli results...................................................................................................................... 22 Figure 5: Week 3 E. coli results...................................................................................................................... 23 Figure 6: Week 4 E. coli results...................................................................................................................... 24 Figure 7: Week 5 E. coli results...................................................................................................................... 25 Figure 8: Week 6 E. coli results...................................................................................................................... 26 Figure 9: Week 7 E. coli results...................................................................................................................... 27 Figure 10: Week 8 E. coli results.................................................................................................................... 28 Figure 11: Week 9 E. coli results.................................................................................................................... 29 Figure 12: Week 10 E. coli results.................................................................................................................. 30 Figure 13: Week 11 E. coli results.................................................................................................................. 31 Figure 14: Week 12 E. coli results.................................................................................................................. 32 Figure 15: Week 13 E. coli results.................................................................................................................. 33 Figure 16: Week 14 E. coli results.................................................................................................................. 34 Figure 17: Week 15 E. coli results.................................................................................................................. 35 Figure 18: Week 16 E. coli results.................................................................................................................. 36 Figure 19: Week 17 E. coli results.................................................................................................................. 37 Figure 20: Week 18 E. coli results.................................................................................................................. 38 Figure 21: Water quality exceedance summary: total body contact daily maximum ........................... 39 Figure 22: Water quality exceedance summary: total body contact 30-day geometric mean .............. 40 Figure 23: Water quality exceedance summary: partial body contact daily maximum........................ 41 APPENDICES Appendix A Global Positioning System coordinates of sampling locations Appendix B Description of sampling locations Appendix C Quality Assurance Project Plan (QAPP) Appendix D Complete E. coli sampling results Appendix E Precipitation records Appendix F Summary of deviations from the QAPP ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT FINAL REPORT PAGE ii ACRONYMS AND ABBREVIATIONS ARL LSSU Aquatic Research Laboratory BST Bacterial Source Tracking CFU Colony Forming Unit CN Copy Number CSO Combined Sewer Overflow EAL LSSU Environmental Analysis Laboratory E. coli Escherichia coli EPA United States Environmental Protection Agency ERP Ecological Research Partners GLWI UWM-Great Lakes Water Institute GPS Global Positioning System LSSU Lake Superior State University MARS Montgomery Associates Resource Solutions mL Milliliter MDNRE Michigan Department of Natural Resources and Environment NOAA National Oceanic and Atmospheric Administration QA Quality Assurance QAPP Quality Assurance Project Plan QC Quality Control qPCR Quantitative Polymerase Chain Reaction SOP Standard Operating Procedure SSO Sanitary Sewer Overflow TMDL Total Maximum Daily Load. UWM University of Wisconsin – Milwaukee ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT FINAL REPORT PAGE iii This page intentionally left blank to facilitate double-sided printing. ST. MARYS RIVER MONITORING PROJECT FOR TMDL DEVELOPMENT FINAL REPORT PAGE iv 1. PROJECT SUMMARY 1.1. PROJECT PURPOSE The main purpose of this project was to collect Escherichia coli (E. coli) data from the main stem of the St. Marys River and its tributaries in an effort to characterize the bacterial contamination in the area around