Norman Wagner

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Norman Wagner Amphibienschutz und Pestizideinsätze Vom Fachbereich VI (Raum- und Umweltwissenschaften) der Universität Trier zur Erlangung des akademischen Grades Doktor der Naturwissenschaften (Dr. rer. nat.) genehmigte Dissertation vorgelegt von Norman Wagner Betreuender: PD Dr. Stefan Lötters Berichterstattende: PD Dr. Stefan Lötters und Prof. Dr. Reinhard Hendler Datum der wissenschaftlichen Aussprache: 16. April 2015 Trier, Juni 2015 Diese Dissertation ist auf dem Online-Publikations-Server (OPUS) der Universität Trier elektronisch publiziert. Holotypus von Xenopus laevis. Aus: DAUDIN, F.-M. (1802): An. XI. Histoire Naturelle des Rainettes, des Grenouilles et des Crapauds. Quarto version. – Levraut (Paris). I Inhaltsverzeichnis Verwendete Abkürzungen ..................................................................................................................... IV Danksagungen ...................................................................................................................................... VII Einleitende Worte ................................................................................................................................ VIII 1. Naturwissenschaftlicher Teil ......................................................................................................... 15 Einleitung .......................................................................................................................................... 15 Weltweite Populationsrückgänge und Artensterben bei Amphibien ............................................. 15 Vorkommen von Amphibien in landwirtschaftlich genutzten Gebieten ....................................... 16 Expositionspfade gegenüber Pestiziden ........................................................................................ 18 Effekte von Pestiziden auf Amphibien .......................................................................................... 19 Der potenzielle Einfluss glyphosatbasierter Herbizide auf Amphibien ......................................... 21 Bewertung des Pestizid-Expositionsrisikos der Amphibienarten des Anhangs II der FFH- Richtlinie in ihren Schutzgebieten des Natura 2000-Netzwerkes ................................................. 22 Laborexperimente mit den beiden Anuren-Modellorganismen Xenopus laevis und Discoglossus scovazzi .......................................................................................................................................... 24 Rezente Einflüsse landwirtschaftlicher Nutzung auf die Ausbildung von Deformationen bei frühen Entwicklungsstadien des Grasfrosches (Rana temporaria) ............................................... 28 Verhaltensversuche zur potenziellen Meidung von kontaminierten Gewässern durch Amphibien ....................................................................................................................................................... 30 In der Einleitung verwendete Literatur .......................................................................................... 31 Kapitel I: Risikobewertungen ............................................................................................................ 45 Questions concerning the potential impact of glyphosate-based herbicides on amphibians ......... 45 Evaluating the risk of pesticide exposure for amphibian species listed in Annex II of the European Union Habitats Directive ............................................................................................................... 85 Kapitel II: Laborexperimente .......................................................................................................... 115 Acute toxic effects of the herbicide formulation used in cycloxydim-tolerant maize cultivation on embryos and larvae of the African clawed frog, Xenopus laevis................................................. 115 Acute toxic effects of the herbicide formulation Focus® Ultra on embryos and larvae of the Moroccan painted frog, Discoglossus scovazzi ........................................................................... 128 No developmental effects but time-lag mortality of environmentally relevant Focus® Ultra concentrations on Xenopus laevis larvae. .................................................................................... 148 Kapitel III: Freilandarbeiten ............................................................................................................ 162 Are deformation rates of anuran developmental stages suitable indicators for environmental pollution? Possibilities and limitations ........................................................................................ 162 Effects of water contamination on site selection by amphibians: experiences from an arena approach with European frogs and newts .................................................................................... 186 Zusammenfassung ........................................................................................................................... 201 In der Zusammenfassung verwendete Literatur .............................................................................. 208 II Schlussfolgerungen.......................................................................................................................... 211 2. Rechtswissenschaftlicher Teil ..................................................................................................... 216 Vermeidung schädlicher Stoffeinträge in Fortpflanzungsgewässer von Amphibien durch Gewässerrandstreifenschutz ................................................................................................................ 216 1. Einleitung .................................................................................................................................... 216 1.1. Gewässerrandstreifen .......................................................................................................... 216 1.2. Rechtlicher Schutzstatus der einheimischen Amphibien und ihr Vorkommen in landwirtschaftlich genutzten Gebieten ............................................................................................ 225 2. Rechtliche Vorgaben zu Gewässerrandstreifen ........................................................................... 233 2.1. Europäische Rechtsgrundlage ............................................................................................. 233 2.2. Nationale Rechtsgrundlage .................................................................................................. 234 2.3. Umsetzung durch landesrechtliche Regelungen .................................................................. 238 2.3.1. Hoher Umsetzungsstandard ......................................................................................... 241 2.3.2. Mittlerer Umsetzungsstandard ..................................................................................... 243 2.3.3. Niedriger Umsetzungsstandard .................................................................................... 245 2.4. Formulierungsspezifische Vorgaben nach dem Pflanzenschutzgesetz ................................ 248 3. Rechtliche Bewertung von Beeinträchtigungen durch den Einsatz von Pflanzenschutz- und Düngemittel aus Sicht des Artenschutzrechtes .................................................................................... 250 3.1. Verbotstatbestände nach § 44 BNatSchG ............................................................................ 250 3.2. Möglichkeiten zu verbesserten Vermeidungs- und Minimierungsmaßnahmen .................. 253 3.2.1. Ausweisung von Gewässerrandstreifen um Amphibienlaichgewässer ....................... 253 3.2.2. Amphibienmonitoring ................................................................................................. 254 3.2.3. Gewässerrandstreifen und Minderertragsareale für den Amphibienschutz ................. 255 4. Zusammenfassung des rechtswissenschaftlichen Teils ............................................................... 256 5. Literaturverzeichnis des rechtswissenschaftlichen Teils ............................................................. 257 Anhänge ............................................................................................................................................... 263 Curriculum Vitae ................................................................................................................................. 386 Erklärung ............................................................................................................................................. 387 III Verwendete Abkürzungen ABl. EG Amtsblatt der Europäischen Gemeinschaft ABl. EU Amtsblatt der Europäischen Union Abs. Absatz AMPA AminoMethylPhosphonic Acid ANOVA ANalysis Of VAriance Art. Artikel ASTM American Society for Testing and Materials BGBl. Bundesgesetzblatt BfN Bundesamt für Naturschutz BMU Bundesministerium für Umwelt, Naturschutz und Reaktorsicherheit BNatSchG Bundesnaturschutzgesetz BVL Bundesamt für Verbraucherschutz und Lebensmittelsicherheit bzw. beziehungsweise cf. vergleiche (aus dem Lateinischen confer) DFG Deutsche Forschungsgemeinschaft EC Effective Concentration (Wirkstoffkonzentration, welche bei einem bestimmten Prozentsatz der Versuchsorganismen innerhalb einer bestimmten Zeit einen definierten Effekt bewirkt) EG Europäische Gemeinschaft e.g. exempli gratia (Latein: zum Beispiel) EU Europäische Union EWG Europäische Wirtschaftsgemeinschaft et al. et alii / et aliae
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