For research use only use research For raldehyde raldehyde and 0.3 mM
rate and immediately mix mix by and rate immediately rious concentrations rious (2.5 concentrations g,
nutes. nutes. ntainer. The final concentrations ntainer. The final concentrations (Cat. No. 6333-50) No. (Cat. (Cat. No. 6339-50) No. (Cat. (Cat. No. 6337-50) No. (Cat. (Cat. No. 6336-50) No. (Cat. (Cat. No. 6335-50) No. (Cat. (Cat. No. 6334-50) No. (Cat. (Cat. No. No. 6332-100) (Cat. Aldose Reductase, human recombinant recombinant human Reductase, Aldose : 408-493-1800 | Fax: 408-493-1801 408-493-1801 Fax: | 408-493-1800 :
www.biovision.com | [email protected] | [email protected] www.biovision.com
AKR1B10 recombinant Human ALDH2 recombinant Human AKR1C3 recombinant Human ALDH3A1 recombinant Human AKR1C1 recombinant Human AKR7A2 recombinant Human AKR7A3 recombinant Human 5 g, 10 g) in 750 l reaction buffer. buffer. reaction l 750 in g) 10 5 g, • are are 0.1 M sodium phosphate (pH 7.0), 10 mM DL glyce NADPH. inversion. • • • •
• • 2/14 2/14
3. mi for 2.5 25°C at Incubate and inversion by Mix 4. Add 200 las a of subst mM 50 DL glyceraldehyde 2. Add 50 l of solution AKR1B1 recombinant with va 5. minutes. 3 for nm in 340 increase the Record 1. a Prepare 750 l reaction intomix a suitable co
ASSAY: ACTIVITY PRODUCTS: RELATED FOR RESEARCH USE ONLY! Not to be used in humans. in used be to Not ONLY! USE RESEARCH FOR Tel
P P LE LE LW LW g g term . Enzymatic LSSQDMTTLL LSSQDMTTLL LAL method) method) LAL keto keto reductase
reduction of a wide variety of ein ein was expressed in E.coli and nzyme nzyme catalyzing the oxidation of 1 alyzing alyzing the reduction of glucose to g g alcohols. This protein is implicated expressed in units/mg of protein. of protein. units/mg in expressed iques. iques. in in 20 mM Tris HCl buffer (pH 8.0) repeated freezing and thawing cycles. cycles. thawing and freezing repeated CA 95035 USA USA 95035 CA
containing 1 mM DTT and 10% glycerol. glycerol. 10% and mM DTT 1 containing E. coli coli E. SDS PAGE by > 95% 1 mg/ml 7361 100 7361 100 g 100 Aldo keto reductase family1, member B1, B1, member family1, reductase Aldo keto AR ALR2, ALDR1, ADR, AKR1B1,
MASRLLLNNG MASRLLLNNG AKMPILGLGT WKSPPGQVTE
Can Can be stored at 4°C short term (1 2 weeks). For lon Specific Specific activity is approximately 0.2 0.9 units/mg
Incorporated BioVision < 1.0 EU per 1 microgram of protein (determined by (determined protein of microgram 1 per EU < 1.0 35.8 kDa (316 aa, 1 316 aa), confirmed by MALDI TOF. by confirmed aa), 1 316 aa, (316 kDa 35.8 : Aldose reductase (AKR1B1) is a member of the aldo Liquid
(AKR) (AKR) superfamily and catalyzes the NADPH dependent compounds to carbonyl containing their correspondin in the development of diabetic complications by cat sorbitol. Recombinant Aldose reductase (AKR1B) prot techn chromatography conventional using by purified AMINO ACID SEQUENCE: CTYHEKGLVK CTYHEKGLVK GACQKTLSDL VVPSDTNILD KLDYLDLYLI TWAAMEELVD HWPTGFKPGK EGLVKAIGIS EFFPLDESGN NFNHLQVEMI LNKPGLKYK DPRIKAIAAK DPRIKAIAAK HNKTTAQVLI IPKSVTPERI RFPMQRNLVV AENFKVFDFE PFHEEF LLSCTSHKDY SYNRNWRVCA BIOLOGICAL ACTIVITY: storage, aliquot and store at 20°C or 70°C. Avoid 70°C. or 20°C at store and aliquot storage, DESCRIPTION activity activity was confirmed by measuring the amount of e was activity Specific 25°C. at NADPH/min micromole STORAGE CONDITIONS: WEIGHT: MOL. LEVEL: ENDOTOXIN Reductase, Aldose human recombinant #: CATALOG SOURCE: PURITY: FORM: FORMULATION: ALTERNATE NAMES: NAMES: ALTERNATE 155 S. Milpitas Boulevard, Milpitas, Boulevard, Milpitas S. 155 AVKVAIDVGY AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSK BioVision BioVision AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSL