FKBP2 Antibody Cat

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FKBP2 Antibody Cat. No.: 23-414 FKBP2 Antibody Western blot analysis of extracts of various cell lines, using FKBP2 antibody (23-414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 22-142 IMMUNOGEN: of human FKBP2 (NP_004461.2). TESTED APPLICATIONS: IF, IHC, WB October 2, 2021 1 https://www.prosci-inc.com/fkbp2-antibody-23-414.html WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:100 - 1:200 IF: ,1:50 - 1:200 POSITIVE CONTROL: 1) MCF7 2) SKOV3 3) Jurkat 4) HeLa 5) Mouse thymus 6) Mouse liver PREDICTED MOLECULAR Observed: 14kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: FKBP2 FKBP-13, FKBP13, PPIase, peptidyl-prolyl cis-trans isomerase FKBP2, 13 kDa FK506-binding protein, 13 kDa FKBP, FK506 binding protein 2, 13kDa, FK506-binding protein 2, PPIase ALTERNATE NAMES: FKBP2, epididymis secretory sperm binding protein, immunophilin FKBP13, proline isomerase, rapamycin-binding protein, rotamase GENE ID: 2286 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References October 2, 2021 2 https://www.prosci-inc.com/fkbp2-antibody-23-414.html The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the BACKGROUND: immunosuppressants FK506 and rapamycin. It is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Multiple alternatively spliced variants, encoding the same protein, have been identified. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 3 https://www.prosci-inc.com/fkbp2-antibody-23-414.html.

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Human FKBP2 Natural ORF Mammalian Expression Plasmid
Human FKBP2 natural ORF mammalian expression plasmid Catalog Number: HG12433-UT General Information Plasmid Resuspension protocol Gene : FK506 binding protein 2, 13kDa 1.Centrifuge at 5,000×g for 5 min. Official Symbol : FKBP2 2.Carefully open the tube and add 100 l of sterile water to Synonym : PPIase, FKBP-13 dissolve the DNA. Source : Human 3.Close the tube and incubate for 10 minutes at room temperature. cDNA Size: 429bp 4.Briefly vortex the tube and then do a quick spin to concentrate RefSeq : BC003384 the liquid at the bottom. Speed is less than 5000×g. Plasmid pCMV3-FKBP2 5.Store the plasmid at -20 ℃. Description Lot : Please refer to the label on the tube The plasmid is ready for: Sequence Description : • Restriction enzyme digestion Identical with the Gene Bank Ref. ID sequence. • PCR amplification Restriction site: HindIII + XbaI (6.1kb + 0.43kb) • E. coli transformation Vector : pCMV3-untagged • DNA sequencing Shipping carrier : Each tube contains approximately 10 μg of lyophilized plasmid. E.coli strains for transformation (recommended but not limited) Storage : Most commercially available competent cells are appropriate for The lyophilized plasmid can be stored at ambient temperature for three months. the plasmid, e.g. TOP10, DH5α and TOP10F´. Quality control : The plasmid is confirmed by full-length sequencing with primers in the sequencing primer list. Sequencing primer list : pCMV3-F： 5’ CAGGTGTCCACTCCCAGGTCCAAG 3’ pcDNA3-R ： 5’ GGCAACTAGAAGGCACAGTCGAGG 3’ Or Forward T7 ： 5’ TAATACGACTCACTATAGGG 3’ ReverseBGH ： 5’ TAGAAGGCACAGTCGAGG 3’ pCMV3-F and pcDNA3-R are designed by Sino Biological Inc. Customers can order the primer pair from any oligonucleotide supplier.
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Tumor Growth and Cancer Treatment
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Protein Symbol Protein Name Rank Metric Score 4F2 4F2 Cell-Surface
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• • • • • • • • • • Po3baxleu: EEF1A1
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