GLIPR1 Antibody

Product Datasheet GLIPR1 Antibody Catalog No: #36505 Orders: [email protected] Description Support: [email protected] Product Name GLIPR1 Antibody Host Species Rabbit Clonality Polyclonal Purification Antigen affinity purification. Applications WB IHC Species Reactivity Hu Ms Specificity The antibody detects endogenous levels of total GLIPR1 protein. Immunogen Type Recombinant Protein Immunogen Description Fusion protein corresponding to a region derived from internal residues of human GLI pathogenesis-related 1 Target Name GLIPR1 Other Names GLIPR; RTVP1; CRISP7 Accession No. Swiss-Prot#: P48060NCBI Gene ID: 11010Gene Accssion: BC012510 SDS-PAGE MW 30kd Concentration 2.6mg/ml Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C Application Details Western blotting: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Images Gel: 10%SDS-PAGE Lysates (from left to right): Mouse kidney tissue Amount of lysate: 40ug per lane Primary antibody: 1/450 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #36505 at dilution 1/60. Background This gene encodes a protein with similarity to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family. Increased expression of this gene is associated with myelomocytic differentiation in macrophage and decreased expression of this gene through gene methylation is associated with prostate cancer. The protein has proapoptotic activities in prostate and bladder cancer cells. This gene is a member of a cluster on chromosome 12 containing two other similar genes. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2.

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Aneuploidy: Using Genetic Instability to Preserve a Haploid Genome?
Health Science Campus FINAL APPROVAL OF DISSERTATION Doctor of Philosophy in Biomedical Science (Cancer Biology) Aneuploidy: Using genetic instability to preserve a haploid genome? Submitted by: Ramona Ramdath In partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biomedical Science Examination Committee Signature/Date Major Advisor: David Allison, M.D., Ph.D. Academic James Trempe, Ph.D. Advisory Committee: David Giovanucci, Ph.D. Randall Ruch, Ph.D. Ronald Mellgren, Ph.D. Senior Associate Dean College of Graduate Studies Michael S. Bisesi, Ph.D. Date of Defense: April 10, 2009 Aneuploidy: Using genetic instability to preserve a haploid genome? Ramona Ramdath University of Toledo, Health Science Campus 2009 Dedication I dedicate this dissertation to my grandfather who died of lung cancer two years ago, but who always instilled in us the value and importance of education. And to my mom and sister, both of whom have been pillars of support and stimulating conversations. To my sister, Rehanna, especially- I hope this inspires you to achieve all that you want to in life, academically and otherwise. ii Acknowledgements As we go through these academic journeys, there are so many along the way that make an impact not only on our work, but on our lives as well, and I would like to say a heartfelt thank you to all of those people: My Committee members- Dr. James Trempe, Dr. David Giovanucchi, Dr. Ronald Mellgren and Dr. Randall Ruch for their guidance, suggestions, support and confidence in me. My major advisor- Dr. David Allison, for his constructive criticism and positive reinforcement.
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Role and Regulation of the P53-Homolog P73 in the Transformation of Normal Human Fibroblasts
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Anti-GLIPR1 Antibody Ab118382
Product Datasheet Anti-GLIPR1 antibody ab118382 3 Images Overview Product name Anti-GLIPR1 antibody Description Mouse monoclonal to GLIPR1 Tested applications WB, ELISA, Flow Cyt Species reactivity Reacts with: Human Immunogen Recombinant fragment, corresponding to amino acids 23-100 of Human GLIPR1 with proprietary tag (NP_006842). Positive control HeLa whole cell lysate. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Storage buffer pH: 7.20 Constituent: 99% PBS Purity Protein A purified Clonality Monoclonal Isotype IgG2a Light chain type kappa Research Areas Cell Biology Apoptosis Intracellular p53 Pathway Cancer Oncoproteins/suppressors Tumor suppressors p53 pathway Cancer Invasion/microenvironment Apoptosis Other Applications Our Abpromise guarantee covers the use of ab118382 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Notes WB WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa. ELISA ELISA: Use at an assay dependent concentration. Flow Cyt Flow Cyt: Use 0.1µg for 106 cells. Target Tissue specificity According to PubMed:8973356, it is ubiquitously expressed with high levels in lung and kidney and low levels in heart and liver. Highly expressed in cell lines derived from nervous system tumors arising from glia, low or absent in non-glial-derived nervous system tumor cell lines. Also found in fetal kidney. According to PubMed:7607567 it is expressed only in brain tumor glioblastoma multiforme/astrocytoma and not in other nervous system tumors or normal fetal or adult tissues.
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Casein Kinase 1Α: Biological Mechanisms and Theranostic Potential Shaojie Jiang1†, Miaofeng Zhang2†, Jihong Sun1 and Xiaoming Yang1,3*
Jiang et al. Cell Communication and Signaling (2018) 16:23 https://doi.org/10.1186/s12964-018-0236-z REVIEW Open Access Casein kinase 1α: biological mechanisms and theranostic potential Shaojie Jiang1†, Miaofeng Zhang2†, Jihong Sun1 and Xiaoming Yang1,3* Abstract Casein kinase 1α (CK1α) is a multifunctional protein belonging to the CK1 protein family that is conserved in eukaryotes from yeast to humans. It regulates signaling pathways related to membrane trafficking, cell cycle progression, chromosome segregation, apoptosis, autophagy, cell metabolism, and differentiation in development, circadian rhythm, and the immune response as well as neurodegeneration and cancer. Given its involvement in diverse cellular, physiological, and pathological processes, CK1α is a promising therapeutic target. In this review, we summarize what is known of the biological functions of CK1α, and provide an overview of existing challenges and potential opportunities for advancing theranostics. Keywords: Casein kinase 1α,Wnt/β-catenin signaling, NF-κB signaling, Hedgehog signaling, Autophagy, Neurodegenerative disease, Cell cycle, Host defense response Background deletion of chromosome 5q [MDS del(5q)] that exerts Casein kinase 1α (CK1α)(encodedbyCSNK1A1 in its effects by inducing CK1α ubiquitination and degrad- humans) is a member of the CK1 family of proteins that ation [12, 13]. These findings suggest that CSNK1A1 is has broad serine/threonine protein kinase activity [1–4] a conditionally essential malignancy gene and a poten- (Fig. 1a) and is one of the main components of the Wnt/ tial target for anti-cancer drugs. β-catenin signaling pathway. CK1α phosphorylates β-catenin at Ser45 as part of the β-catenin destruction com- α plex for subsequent β-transducin repeat-containing E3 ubi- Overview of CK1 quitin protein ligase (β-TrCP)-mediated ubiquitination and CSNK1A1 is located on chromosome 5q32 and is proteasomal degradation [5, 6].
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