<<

碧云天生物技术/Beyotime Biotechnology 订货热线:400-168-3301或800-8283301 订货e-mail:[email protected] 技术咨询:[email protected] 碧云天网站 微信公众号 网址:http://www.beyotime.com

Hoechst 33258

产品编号 产品名称 包装 C1011 Hoechst 33258 10mg

产品简介:

 Hoechst 33258,也称bisBenzimide H 33258或HOE 33258。分子式为C25H24N6O·3HCl,分子量为533.88,CAS Number 23491-45-4。  Hoechst 33258是一种可以穿透细胞膜的蓝色荧光染料,对细胞的毒性较低。  Hoechst 33258染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33258也常用于普通的细胞 核染色,或常规的DNA染色。  Hoechst 33258的最大激发波长为346nm,最大发射波长为460nm;Hoechst 33258和双链DNA结合后,最大激发波长为352nm, 最大发射波长为461nm。  Hoechst 33258溶于水,溶解度可达10mg/ml。  用于细胞核染色时,推荐的Hoechst 33258工作浓度为0.5-10µg/ml。 包装清单: 产品编号 产品名称 包装 C1011 Hoechst 33258 10mg — 说明书 1份 保存条件: -20ºC避光保存,一年有效。 注意事项:  荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。  为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。  Hoechst 33258对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。  本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。  为了您的安全和健康,请穿实验服并戴一次性手套操作。 相关产品: 产品编号 产品名称 包装 C1002 DAPI 5mg/ml×0.2ml C1005 DAPI 染色液 10ml C1006 DAPI 染色液 50ml C1011 Hoechst 33258 10mg C1017 Hoechst 33258 染色液 10ml C1018 Hoechst 33258 染色液 50ml C1022 Hoechst 33342 10mg C1025 Hoechst 33342 染色液 10ml C1026 Hoechst 33342 染色液 50ml C1027 Hoechst 33342 活细胞染色液(100X) 0.1ml C1028 Hoechst 33342 活细胞染色液(100X) 0.5ml C1029 Hoechst 33342 活细胞染色液(100X) 3ml 使用本产品的文献: 1. Yao G, Yang L, Hu Y, Liang J, Liang J, Hou Y. Nonylphenol-induced nucleopolyhedrovirus. Virus Res. 2006 Mar;116(1-2):129-35. thymocyte apoptosis involved caspase-3 activation and mitochondrial 3. Li HG, Liao AH, Ding XF, Zhou H, Xiong CL. The expression and depolarization. Mol Immunol. 2006 Mar;43(7):915-26. significance of CATSPER1 in human testis and ejaculated spermatozoa. 2. Nie Y, Wang Q, Liang C, Fang M, Yu Z, Chen X. Characterization of Asian J Androl. 2006 May;8(3):301-6. ORF2 and its encoded protein of the Helicoverpa armigera 4. Yao G, Ling L, Luan J, Ye D, Zhu P. Nonylphenol induces apoptosis of Jurkat cells by a caspase-8 dependent mechanism. Int Immunopharmacol. apoptosis. J Hepatol. 2009 Apr;50(4):766-78. 2007 Apr;7(4):444-53. 26. Zhu C, Huang H, Hua R, Li G, Yang D, Luo J, Zhang C, Shi L, Benovic 5. Gao MQ, Guo SB, Chen XH, Du W, Wang CB. Molecular mechanisms of JL, Zhou N. Molecular and functional characterization of adipokinetic polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis hormone receptor and its ligands in Bombyx mori. FEBS Lett. induced by UVA plus UVB. Acta Pharmacol Sin. 2007 Jul;28(7):1007-14. 2009 May 6;583(9):1463-8. 6. Bao W, Pan H, Lu M, Ni Y, Zhang R, Gong X. The apoptotic effect of 27. Wang H, Xu Y, Yan J, Zhao X, Sun X, Zhang Y, Guo J, Zhu C. Acteoside sarsasapogenin from Anemarrhena asphodeloides on HepG2 human protects human neuroblastoma SH-SY5Y cells against beta-amyloid hepatoma cells. Cell Biol Int. 2007 Sep;31(9):887-92. -induced cell injury. Brain Res. 2009 Aug 4;1283:139-47. 7. Li X, Peng C, Li L, Ming M, Yang D, Le W. Glial cell-derived 28. Zeng X, Nan F, Liang C, Song J, Wang Q, Vlak JM, Chen X. Functional neurotrophic factor protects against proteasome inhibition-induced analysis of the Autographa californica nucleopolyhedrovirus IAP1 and dopamine neuron degeneration by suppression of endoplasmic reticulum IAP2. Sci China C Life Sci. 2009 Aug;52(8):761-70. stress and caspase-3 activation. J Gerontol A Biol Sci Med Sci. 2007 29. Shen L, Liu Q, Ni J, Hong G. A proteomic investigation into the human Sep;62(9):943-50. cervical cancer cell line HeLa treated with dicitratoytterbium (III) 8. Yan XB, Yang DS, Gao X, Feng J, Shi ZL, Ye Z. Caspase-8 dependent complex. Chem Biol Interact. 2009 Oct 30;181(3):455-62. osteosarcoma cell apoptosis induced by proteasome inhibitor MG132. Cell 30. Guo CJ, Pan Q, Jiang B, Chen GY, Li DG. Effects of upregulated Biol Int. 2007 Oct;31(10):1136-43. expression of microRNA-16 on biological properties of culture-activated 9. Lin YF, Zhang N, Guo HS, Kong DS, Jiang T, Liang W, Zhao ZH, Tang hepatic stellate cells. Apoptosis. 2009 Nov;14(11):1331-40. QQ, Ma D. Recombinant tissue factor pathway inhibitor induces apoptosis 31. Yu M, Cai L, Liang M, Huang Y, Gao H, Lu S, Fei J, Huang F. Alteration in cultured rat mesangial cells via its Kunitz-3 domain and C-terminal of NRSF expression exacerbating 1-methyl-4-phenyl-pyridinium through inhibiting PI3-kinase/Akt pathway. Apoptosis. 2007 ion-induced cell death of SH-SY5Y cells. Neurosci Res. 2009 Nov; Dec;12(12):2163-73. 65(3):236-44. 10. Yao H, Tao Z, Ai M, Peng L, Wang G, He B, Li Y. Raman spectroscopic 32. Zhuo L, Gong J, Yang R, Sheng Y, Zhou L, Kong X, Cao K. Inhibition of analysis of apoptosis of single human gastric cancer cells. Vibrational proliferation and differentiation and promotion of apoptosis by cyclin L2 Spectroscopy.2008 Nov;50(2009):193–7. in mouse embryonic carcinoma P19 cells. Biochem Biophys Res 11. Wu G, Liu ZS, Qian Q, Jiang CQ. Effects of on the Growth of Commun. 2009 Dec 18;390(3):451-7. Hepatocellular Carcinoma Cell lines. Medical Journal of Wuhan 33. Zhang S, Wang J, Song N, Xie J, Jiang H. Up-regulation of divalent metal University. 2008 Jan;29(1)102-5. transporter 1 is involved in 1-methyl-4-phenylpyridinium 12. Wang Q, Wang Y, Liang C, Song J, Chen X. Identification of a (MPP(+))-induced apoptosis in MES23.5 cells. Neurobiol Aging. 2009 hydrophobic domain of HA2 essential to morphogenesis of Helicoverpa Sep;30(9):1466-76. armigera nucleopolyhedrovirus. J Virol. 2008 Apr;82(8):4072-81. 34. Wang HQ, Xu YX, Zhao XY, Zhao H, Yan J, Sun XB, Guo JC, Zhu CQ. 13. Xu HM, Jiang H, Wang J, Luo B, Xie JX. Over-expressed human divalent Overexpression of F(0)F(1)-ATP synthase alpha suppresses mutant metal transporter 1 is involved in iron accumulation in MES23.5 cells. huntingtin aggregation and toxicity in vitro. Biochem Biophys Res Neurochem Int. 2008 May;52(6):1044-51. Commun. 2009;390(4):1294-8. 14. Chen P, Yang LL, Yang HS, Wang YS, Li G, Wu Y, Fang F, Liu K, Li J, 35. Li G,Shi H,Huang H,Zhang Y,Wu K,Luo J,Sun Y, Lu J, L. Benovic J and Zhao X, Hu HZ, Wei YQ. Synergistic antitumor effect of CXCL10 with Zhou N. Internalization of the human nicotinic acid receptor GPR109A is hyperthermia. J Cancer Res Clin Oncol. 2008 Jun;134(6):679-87. regulated by Gi, GRK2,and arrestin3. The Journal of Biological. 2010 Jul 15. Shen H, Qin H, Guo J. Cooperation of metallothionein and zinc 16;285(29):22605-18. transporters for regulating zinc homeostasis in human intestinal Caco-2 36. Li B, Qi XQ, Chen X, Huang X, Liu GY, Chen HR, Huang CG, Luo C, Lu cells. Nutr Res. 2008 Jun;28(6):406-13. YC. Expression of targeting protein for Xenopus kinesin-like protein 2 is 16. Qin L, Zhang X, Zhang L, Feng Y, Weng GX, Li MZ, Kong QL, Qian CN, associated with progression of human malignant astrocytoma. Brain Res. Zeng YX, Zeng MS, Liao DF, Song LB. Downregulation of BMI-1 2010;1352:200-7. enhances 5-fluorouracil-induced apoptosis in nasopharyngeal carcinoma 37. Yan YJ, Zheng MZ, Chen ZL, Yu XH, Yang XX, Ding ZL, Xu L. Studies cells. Biochem Biophys Res Commun. 2008 Jul 4;371(3):531-5. on preparation and photodynamic mechanism of chlorin 17. Zhou J, Ye J, Zhao X, Li A, Zhou J. JWA is required for arsenic trioxide P6-13,15-N-(cyclohexyl)cycloimide (Chlorin-H) and its antitumor effect induced apoptosis in HeLa and MCF-7 cells via reactive oxygen species for photodynamic therapy in vitro and in vivo. Bioorg Med Chem. and mitochondria linked signal pathway. Toxicol Appl Pharmacol. 2008 2010;18(17):6282-91. Jul 1;230(1):33-40. 38. Xu H, Yan Y, Li L, Peng S, Qu T, Wang B. Ultraviolet B-induced 18. Yang X, Zhang Y, Zhang L, Zhang L, Mao J. Silencing alpha-fetoprotein apoptosis of human skin fibroblasts involves activation of caspase-8 and expression induces growth arrest and apoptosis in human hepatocellular -3 with increased expression of vimentin. Photodermatol Photoimmunol cancer cell. Cancer Lett. 2008 Nov 28;271(2):281-93. Photomed. 2010 Aug;26(4):198-204. 19. Zhang PY, Zhang WG, He AL, Wang JL, Li WB. Identification and 39. Ma J, Lu HY, Xia YK, Dong HB, Gu AH, Li ZY, Li Z, Chen AM, Wang functional characterization of the novel acute monocytic leukemia XR, Wang SL. BCL2 Ala43Thr is a functional variant associated with associated antigen MLAA-34. Cancer Immunol Immunother. 2009 protection against azoospermia in a Han-Chinese population. Biol Reprod. Feb;58(2):281-90. 2010 Oct;83(4):656-62. 20. Liu X, Zhang Z, Li P, Zhu L, Wang Y, Wang C. Polypeptide from Chlamys 40. Liu JT, Guo X, Ma WJ, Zhang YG, Xu P, Yao JF, Bai YD. Mitochondrial farreri modulates UVB-induced activation of NF-kappaB signaling function is altered in articular chondrocytes of an endemic osteoarthritis, pathway and protection HaCaT cells from apoptosis. Regul Pept. 2009 Kashin-Beck disease. Osteoarthritis Cartilage. 2010 Sep;18(9):1218-26. Feb 25;153(1-3):49-55. 41. Zhu W, Chang Y, Wu C, Han Q, Pei R, Lu M, Chen X. The wild-type 21. Li ZX, Ouyang KQ, Jiang X, Wang D, Hu Y. induces apoptosis hepatitis C virus core inhibits initiation of antigen-specific T- and B-cell and inhibits growth of human Burkitt's lymphoma in xenograft mouse immuneresponses in BALB/c mice. Clin Vaccine Immunol. 2010 model. Mol Cells. 2009 Mar;27(3):283-9. Jul;17(7):1139-47. 22. Gong Y, Wu J, Huang Y, Shen S, Han X. Nonylphenol induces apoptosis 42. Wang J, Han X, Yang H, Lu L, Wu Y, Liu X, Guo R, Zhang Y, Zhang Y, Li in rat testicular Sertoli cells via endoplasmic reticulum stress. Toxicol Q. A novel RGD-toxin protein, Lj-RGD3, from the buccal gland secretion Lett. 2009 Apr 25;186(2):84-95. of Lampetra japonica impactsdiverse biological activities. Biochimie. 2010 Oct;92(10):1387-96 23. Zhang SP, Zhou YJ, Liu Y, Cai YQ. Effect of liquiritigenin, a flavanone existed from Radix glycyrrhizae on pro-apoptotic in SMMC-7721 cells. 43. Shen L, Lan Z, Sun X, Shi L, Liu Q, Ni J. Proteomic analysis of Food Chem Toxicol. 2009 Apr;47(4):693-701. lanthanum citrate-induced apoptosis in human cervical carcinoma SiHacells. Biometals. 2010 Dec;23(6):1179-89. 24. Hui L, Ji C, Hui B, Lv T, Ha X, Yang J, Cai W. The oncoprotein LMO3 interacts with calcium- and -binding protein CIB. Brain Res. 2009 44. Li K, Wang Y, Bai H, Wang Q, Song J, Zhou Y, Wu C, Chen X. The Apr 10;1265:24-9. putative pocket protein binding site of Autographa californica nucleopolyhedrovirus BV/ODV-C42 is required for virus-induced nuclear 25. Guo CJ, Pan Q, Li DG, Sun H, Liu BW. miR-15b and miR-16 are actin polymerization. J Virol. 2010 Aug;84(15):7857-68. implicated in activation of the rat hepatic stellate cell: An essential role for 45. Ren J, Zhu D, Liu M, Sun Y, Tian L. Downregulation of miR-21

2 / 5 C1011 Hoechst 33258 400-1683301/800-8283301 碧云天/Beyotime modulates Ras expression to promote apoptosis and suppress invasionof M, Chen X. Amino acid substitutions at positions 122 and 145 of hepatitis Laryngeal squamous cell carcinoma. Eur J Cancer. 2010;46(18):3409-16. B virus surface antigen (HBsAg)determine the antigenicity and 46. Zhu MT, Wang Y, Feng WY, Wang B, Wang M, Ouyang H, Chai ZF. immunogenicity of HBsAg and influence in vivo HBsAg clearance. J Oxidative stress and apoptosis induced by iron oxide nanoparticles in Virol. 2012 Apr;86(8):4658-69. cultured human umbilicalendothelial cells. J Nanosci Nanotechnol. 2010 65. Di S, Tian Z, Qian A, Li J, Wu J, Wang Z, Zhang D, Yin D, Brandi ML, Dec;10(12):8584-90. Shang P. Large gradient high magnetic field affects FLG29.1 cells 47. Xiong GS, Sun HL, Wu SM, Mo JZ. Small interfering RNA against the differentiation to form osteoclast-like cells. Int J Radiat Biol. 2012 apurinic or apyrimidinic endonuclease enhances the sensitivityof human Nov;88(11):806-13. pancreatic cancer cells to gemcitabine in vitro. J Dig Dis. 2010 66. Zeng F, Ni E, Wang S, Lv S, Cao C, Zhang H, Lv H, Chen X, Yan J, Luo Aug;11(4):224-30. F. A Monoclonal Antibody Against the Catalytic Domain of PTP1B. 48. Peng H, Zhou JL, Liu SQ, Hu QJ, Ming JH and Qiu B. Hyaluronic acid Hybridoma (Larchmt). 2012 Jun;31(3):209-13. inhibits nitric oxide-induced apoptosis and dedifferentiation of articular 67. Xiong H, Du W, Zhang YJ, Hong J, Su WY, Tang JT, Wang YC, Lu R, chondrocytes in vitro. INFLAMMATION RESEARCH. 2010 Fang JY. Trichostatin A, a histone deacetylase inhibitor, suppresses Jul;59(7):519-30. JAK2/STAT3 signaling via inducing the promoter-associated histone 49. Du TT, Li L, Song N, Xie JX, Jiang H. Rosmarinic Acid Antagonized acetylation of SOCS1 and SOCS3 in human colorectal cancer cells. Mol 1-Methyl-4-Phenylpyridinium (MPP+)-Induced Neurotoxicity in MES23. Carcinog. 2012 Feb;51(2):174-84. 5 Dopaminergic Cells. International Journal of Toxicology. 2010 68. Li SZ,Li YH,Li JL,Liu C,Yin W,Zhao QX,Lin Z,Ji H and Yang HM. Dec;29(6):625-33. Construction of pECFP-C1-CIRP and its effect on cold-induced apoptosis 50. Zheng J, Fang J, Yin YJ, Wang XC, Ren AJ, Bai J, Sun XJ, Yuan WJ, Lin in mouse NIH/3T3 cells. African Journal of Biotechnology. 2012 Mar;11(21):4938-48. L. protects cardiomyocytes from serum‐deprivation‐induced apoptosis by increasing anti-oxidant defence. Clinical and Experimental 69. Yuan XH, Lu CL, Yao N, An LS, Yang BQ, Zhang CL, Ma X. Arsenic Pharmacology and Physiology. 2010 Oct;37(10):955-963. induced production in a caspase-3-dependent manner and changed redoxstatus in preovulatory granulosa cells. J Cell Physiol. 2012 51. Wang X,Xu J,Ju S,Ni H,Zhu J,Wang H. Livin gene plays a role in drug Jan;227(1):194-203. resistance of colon cancer cells. Clinical Biochemistry. 2010 May;43(7-8):655–60. 70. Tang W, Zhang Y, Qian C, Yuan Z. Induction and mechanism of apoptosis by hydroxycamptothecin in human Tenon's capsulefibroblasts. Invest 52. Wang Y, Hong C, Zhou C, Xu D, Qu HB, Cheng Y. Screening Antitumor Ophthalmol Vis Sci. 2012 Jul 24;53(8):4874-80. Compounds Psoralen and Isopsoralen from Psoralea corylifolia L. Seeds. Evid Based Complement Alternat Med. 2011;2011:363052 71. Zhang L, Ruan J, Yan L, Li W, Wu Y, Tao L, Zhang F, Zheng S, Wang A, Lu Y. Xanthatin induces cell cycle arrest at G2/M checkpoint and 53. Wang Q, Jiang H, Fan Y, Huang X, Shen J, Qi H, Li Q, Lu X, Shao J. apoptosis via disrupting NF-κB pathwayin A549 non-small-cell lung Phosphorylation of the α-subunit of the eukaryotic initiation factor-2 cancer cells. Molecules.2012 Mar 26;17(4):3736-50. (eIF2α) alleviates benzo[a]pyrene-7,8-diol-9,10-epoxide induced cell cycle arrest and apoptosis in human cells. Environ Toxicol Pharmacol. 72. Jiang C, Zhang S, Liu H, Zeng Q, Xia T, Chen Y, Kuang G, Zhao G, Wu 2011 Jan;31(1):18-24. X, Zhang X, Wang A. The role of the IRE1 pathway in PBDE-47-induced toxicity in human neuroblastoma SH-SY5Y cellsin vitro. Toxicol Lett. 54. Wang F, Ding W, Wang J, Jing R, Wang X, Cong H, Wang Y, Ju S, Wang 2012 Jun 20;211(3):325-33. H. Identification of microRNA-target interaction in APRIL-knockdown colorectal cancer cells. Cancer Gene Ther. 2011 Jul;18(7):500-9. 73. Li XJ, Zhu CD, Yu W, Wang P, Chen FF, Xia XY, Luo B. Overexpression of Id3 induces apoptosis of A549 human lung adenocarcinoma cells. Cell 55. Li DL, Liu JJ, Liu BH, Hu H, Sun L, Miao Y, Xu HF, Yu XJ, Ma X, Ren J, Prolif. 2012 Feb;45(1):1-8. Zang WJ. Acetylcholine inhibits hypoxia-induced tumor necrosis factor-α production via regulation of MAPKsphosphorylation in cardiomyocytes. J 74. Zhou P, Li H, Wang H, Wang LF, Shi Z. Bat severe acute respiratory Cell Physiol. 2011 Apr;226(4):1052-9. syndrome-like coronavirus ORF3b homologues display differentinterferon antagonist activities. J Gen Virol. 2012 Feb;93(Pt 2):275-81. 56. Xu XL, Huang YJ, Wang YQ, Chen XF, Zhang W. 2,3,4',5-Tetrahydroxystilbene-2-O-β-d-glucoside inhibits platelet-derived 75. Liu M, Yang S, Jin L, Hu D, Wu Z, Yang S. Chemical Constituents of the growth factor-inducedproliferation of vascular smooth muscle cells by Ethyl Acetate Extract of Belamcanda chinensis (L.) DCRoots and Their regulating the cell cycle. Clin Exp Pharmacol Physiol. 2011 Antitumor Activities. Molecules. 2012 May 24;17(5):6156-69. May;38(5):307-13. 76. Wang JJ, Li YF, Jin YY, Wang X, Chen TX. Effects of Epstein-Barr virus 57. Jiang QH, Liu L, Shen JW, Peel S, Yang GL, Zhao SF, He FM. Influence on the development of dendritic cells derived from cord bloodmonocytes: of multilayer rhBMP-2 DNA coating on the proliferation and an essential role for apoptosis. Braz J Infect Dis. 2012 Jan-Feb; differentiation of MC3T3-E1cells seeded on roughed titanium surface. J 16(1):19-26. Biomed Mater Res A. 2012 Oct;100(10):2766-74. 77. Zhao P, Han T, Guo JJ, Zhu SL, Wang J, Ao F, Jing MZ, She YL, Wu ZH, 58. He D, Ma X, Chen Y, Cai Y, Ru X, Bruce IC, Xia Q, Shi G, Jin J. Luteolin Ye LB. HCV NS4B induces apoptosis through the mitochondrial death inhibits pyrogallol-induced apoptosis through the extracellular pathway. Virus Res. 2012 Oct;169(1):1-7. signal-regulated kinase signaling pathway. FEBS J. 2012 May; 78. Muhammad Khan, Bin Zheng, Fei Yi, Azhar Rasul, Zhuyi Gu. Pseudolaric 279(10):1834-43. Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis 59. Shi W, Nie D, Jin G, Chen W, Xia L, Wu X, Su X, Xu X, Ni L, Zhang X, in U87 Glioblastoma Cells. Evidence-Based Complementary and Zhang X, Chen J. BDNF blended chitosan scaffolds for human umbilical Alternative Medicine. 2012;2012:957568. cord MSC transplants in traumatic braininjury therapy. Biomaterials. 2012 79. Liu J, Wang J, Zhou Y. Sur-expression de la protéine BNIP3 et adressage Apr;33(11):3119-26. vers la mitochondrie au cours de l’apoptose des cellules du noyau pulpeux 60. Song J, Wang M, Huang H, Luo X, Deng F, Wang H, Hu Z. Functional induite par carence nutritionnelle. Revue du Rhumatisme. 2012 studies of per os infectivity factor 3 of Helicoverpa armigera Mar;79(2):156–61. nucleopolyhedrovirus. J Gen Virol. 2012 Feb;93(Pt 2):374-82. 80. Sun T, Luo J, Jia M, Li H, Li K, Fu Z. Small interfering RNA-mediated 61. Yang S, Ma J, Xiao J, Lv X, Li X, Yang H, Liu Y, Feng S, Zhang Y. knockdown of NF-κBp65 attenuates neuropathic pain following peripheral Anti-Tumor Activity in Bladder Cancer T24 Cell Line Through nerveinjury in rats. Eur J Pharmacol. 2012 May 5;682(1-3):79-85. Induction of Cell-CycleArrest and Apoptosis. Anat Rec (Hoboken). 2012 81. Zhang WF, Xu YY, Xu KP, Wu WH, Tan GS, Li YJ, Hu CP. Inhibitory Aug;295(8):1260-6. effect of selaginellin on high glucose-induced apoptosis in differentiated 62. Yinghui Dong, Hanhan Yao, Zhihua Lin, Dongli Zhu. The effects of PC12 cells: role of NADPHoxidase and LOX-1. Eur J Pharmacol. 2012 sperm–egg ratios on polyspermy in the blood clam,Tegillarca granosa. Nov 5;694(1-3):60-8. Aquaculture Research. 2012 Jun;43(1):44–52. 82. Guo Y, Wang G, Gao WW, Cheng SW, Wang R, Ju SM, Cao HL, Tian HL. 63. Zhong Q, Zhou Y, Ye W, Cai T, Zhang X, Deng DY. Hypoxia-Inducible Induction of apoptosis in glioma cells and upregulation of Fas expression Factor 1-α-AA-Modified Bone Marrow Stem Cells Protect PC12 Cells using the human interferon-β gene. Asian Pac J Cancer Prev. fromHypoxia-Induced Apoptosis, Partially Through 2012;13(6):2837-40. VEGF/PI3K/Akt/FoxO1 Pathway. Stem Cells Dev. 2012 Sep 20; 83. Huang EW, Xue SJ, Zhang Z, Zhou JG, Guan YY, Tang YB. Vinpocetine 21(14):2703-17. inhibits breast cancer cells growth in vitro and in vivo. Apoptosis. 2012 64. Wu C, Deng W, Deng L, Cao L, Qin B, Li S, Wang Y, Pei R, Yang D, Lu Oct;17(10):1120-30.

碧云天/Beyotime 400-1683301/800-8283301 C1011 Hoechst 33258 3 / 5 84. Zhong WX, Wang YB, Peng L, Ge XZ, Zhang J, Liu SS, Zhang XN, Xu of pH-sensitive liposomes based on zwitterionic oligopeptide lipids. ZH, Chen Z, Luo JH. Lanthionine synthetase C-like protein 1 interacts Biomaterials. 2013 Apr;34(11):2773-86. with and inhibits cystathionine β-synthase: a target for 104. Hu X, Li H, Luo S,Liu T,Jiang Y, Liu S. Thiol and pH dual-responsive neuronalantioxidant defense. J Biol Chem. 2012 Oct 5; dynamic covalent shell cross-linked micelles for triggered release of 287(41):34189-201. chemotherapeutic drugs. Polym. Chem. 2013 Sep;3:695-706. 85. Song H, Wang G, He B, Li L, Li C, Lai Y, Xu X, Gu Z. Cationic 105. He B, Jia Z, Du W, Yu C, Fan Y, Dai W, Yuan L, Zhang H, Wang X, Wang lipid-coated PEI/DNA polyplexes with improved efficiency and reduced J, Zhang X, Zhang Q. The transport pathways of polymer nanoparticles in cytotoxicity for gene delivery intomesenchymal stem cells. Int J MDCK epithelial cells. Biomaterials. 2013 Jun;34(17):4309-26. Nanomedicine. 2012;7:4637-48. 106. Wang PH, Gu ZH, Wan DH, Zhu WB, Qiu W, Chen YG, Weng SP, Yu 86. Zhang J, Cai T, Zhao F, Yao T, Chen Y, Liu X, Luo W, Chen J. The role of XQ, He JG. Litopenaeus vannamei Toll-interacting protein (LvTollip) is a α-synuclein and tau hyperphosphorylation-mediated autophagy and potential negative regulator of the shrimp Toll pathway involved in the apoptosis in lead-inducedlearning and memory injury. Int J Biol Sci. regulation of the shrimp antimicrobial peptide gene penaeidin-4 (PEN4). 2012;8(7):935-44. Dev Comp Immunol. 2013 Jul-Aug;40(3-4):266-77. 87. Yang XJ, Lu HY, Li ZY, Bian Q, Qiu LL, Li Z, Liu Q, Li J, Wang X, 107. Wang PH, Gu ZH, Wan DH, Zhu WB, Qiu W, Weng SP, Yu XQ, He JG. Wang SL. Cytochrome P450 2A13 mediates aflatoxin B1-induced Litopenaeus vannamei sterile-alpha and armadillo motif containing cytotoxicity and apoptosis in human bronchial epithelialcells. Toxicology. protein (LvSARM) is involved in regulation of Penaeidins and 2012 Oct 28;300(3):138-48. antilipopolysaccharide factors. PLoS One. 2013;8(2):e52088. 88. Meng J, Zhang Z, Zheng Z, Liu Y, Wang H. -101 from one 108. Cai X,Dong H, Ma J, Zhu H, Wu W, Chu M, Li Y, Shi D. Effects of spatial strain of H5N1 NS1 protein determines its IFN-antagonizing ability and distribution of the nuclear localization sequence on gene transfection in subcellulardistribution pattern. Sci China Life Sci. 2012 Nov; catiomer-gene polyplexes. J. Mater. Chem. 2013 Jan;12:1712-21. 55(11):933-9. 109. Wei F, Li JL, Ling JX, Chen LJ, Li N, Liu YY, Luo F, Xiong HR, Hou W, 89. Li J, Fan Y, Zhang YN, Sun DJ, Fu SB, Ma L, Jiang LH, Cui C, Ding HF, Yang ZQ. Establishment of SYBR green-based qPCR assay for rapid Yang J. The Raf-1 inhibitor GW5074 and the ERK1/2 pathway inhibitor evaluation and quantification for anti-Hantaan viruscompounds in vitro U0126 ameliorate PC12 cells apoptosis induced by6-hydroxydopamine. and in suckling mice. Virus Genes. 2013 Feb;46(1):54-62. Pharmazie. 2012 Aug;67(8):718-24. 110. Chen P, Wang L, Li N, Liu Q, Ni J. Comparative proteomics analysis of 90. Luo M, Weng Y, Tang J, Hu M, Liu Q, Jiang F, Yang D, Liu C, Zhan X, sodium selenite-induced apoptosis in human prostate cancer cells. Song P, Bai H, Li B, Shi Q. MicroRNA-450a-3p represses cell Metallomics. 2013 May 1;5(5):541-50. proliferation and regulates embryo development by regulating Bub1 expressionin mouse. PLoS One. 2012;7(10):e47914. 111. Tian S, Bai Y, Yang L, Wang X, Wu Y, Jia J, Zhu Y, Cheng Y, Zhang P, Wu J, Wang N, Xia G, Liao H, Zhang Y, Shen X, Yu H, Hu Y. Shear stress 91. Kang R, Wang ZH, Wang BQ, Zhang CM, Gao W, Feng Y, Bai T, Zhang inhibits apoptosis of ischemic brain microvascular endothelial cells. Int J HL, Huang-Pu H, Wen SX. Inhibition of autophagy-potentiated Mol Sci. 2013 Jan 11;14(1):1412-27. chemosensitivity to cisplatin in laryngeal cancer Hep-2 cells. Am J Otolaryngol. 2012 Nov-Dec;33(6):678-84. 112. Zhao W, He H, Ren K, Li B, Zhang H, Lin Y, Shao RG. MR-1 blocks the megakaryocytic differentiation and transition of CML from chronic phase 92. Liu J, Wang J, Zhou Y. Upregulation of BNIP3 and translocation to to blast crisis throughMEK dephosphorylation. Blood Cancer J. 2013 Mar mitochondria in nutrition deprivation induced apoptosis in 29;3:e107. nucleuspulposus cells. Joint Bone Spine. 2012 Mar;79(2):186-91. 113. Wu W, Gao X, Xu X, Luo Y, Liu M, Xia Y, Dai Y. Saponin-rich fraction 93. Liu MC, Yang SJ, Jin LH, Hu DY, Xue W, Song BA, Yang S. Synthesis from Clematis chinensis Osbeck roots protects rabbit chondrocytes against and cytotoxicity of novel ursolic acid derivatives containing an acyl nitric oxide-induced apoptosis via preventing mitochondria impairment piperazine moiety. Eur J Med Chem. 2012 Dec;58:128-35. and caspase-3 activation. Cytotechnology. 2013 Mar;65(2):287-95. 94. Yuan T, Luo BL, Wei TH, Zhang L, He BM, Niu RC. Salubrinal protects 114. Zhao J, Wei J, Liu M, Xiao L, Wu N, Liu G, Huang H, Zhang Y, Zheng L, against cigarette smoke extract-induced HBEpC apoptosis likely via Lin X. Cloning, characterization and expression of a cDNA encoding a regulating the activity of PERK-eIF2α signaling pathway. Arch Med Res. granulin-like polypeptide in Ciona savignyi. Biochimie. 2013 2012 Oct;43(7):522-9. Aug;95(8):1611-9. 95. Ye Y, Li Z, Xing D. Nitric oxide promotes MPK6-mediated caspase-3-like 115. Yin J, Wu N, Zeng F, Cheng C, Kang K, Yang H. Paeonol induces activation in cadmium-induced Arabidopsisthaliana programmed cell apoptosis in human ovarian cancer cells. Acta Histochem. 2013 death. Plant Cell Environ. 2013 Jan;36(1):1-15. Oct;115(8):835-9. 96. Liu L, Li W, Wei X, Cui Q, Lou W, Wang G, Hu X, Qian C. Potent 116. Huang H, Shi H, Feng S, Chen W, Yu Y, Lin D, Chen R. Confocal Raman antitumor activity of oncolytic adenovirus-mediated SOCS1 for spectroscopic analysis of the cytotoxic response to cisplatin in hepatocellular carcinoma. Gene Ther. 2013 Jan;20(1):84-92. nasopharyngeal carcinoma cells. Anal. Methods. 2013 Nov;1:260-6. 97. Xu Y, Li Y, Li H, Pang Y, Zhao Y, Jiang R, Shen L, Zhou J, Wang X, Liu 117. Lu J, Miao J, Su T, Liu Y, He R. Formaldehyde induces hyperpho Q. The accumulations of HIF-1α and HIF-2α by JNK and ERK are sphorylation and polymerization of Tau protein both in vitro and in vivo. involved in biphasic effects induced by differentlevels of arsenite in Biochim Biophys Acta. 2013 Aug;1830(8):4102-16. human bronchial epithelial cells. Toxicol Appl Pharmacol. 2013 Jan 15; 266(2):187-97. 118. Pang Y, Xu Y, Li H, Li Y, Zhao Y, Jiang R, Shen L, Zhou J, Wang X, Liu Q. The inhibition of HIF-2α on the ATM/Chk-2 pathway is involved in the 98. Jiang Q, Dai L, Cheng L, Chen X, Li Y, Zhang S, Su X, Zhao X, Wei Y, promotion effect of arsenite onbenzo(a)pyrene-induced cell Deng H. Efficient inhibition of intraperitoneal ovarian cancer growth in transformation. Toxicol Lett. 2013 Apr 12;218(2):105-17. nude mice by liposomal delivery of short hairpinRNA against STAT3. J Obstet Gynaecol Res. 2013 Mar;39(3):701-9. 119. Du Q, Hu B, An HM, Shen KP, Xu L, Deng S, Wei MM. Synergistic anticancer effects of curcumin and in Hepa1-6 hepatocellular 99. Liu Y, Wang W, Fang B, Ma F, Zheng Q, Deng P, Zhao S, Chen M, Yang carcinoma cells. Oncol Rep. 2013 May;29(5):1851-8. G, He G. Anti-tumor effect of germacrone on human hepatoma cell lines through inducing G2/M cell cycle arrest andpromoting apoptosis. Eur J 120. Wang YQ, Chen C, Chen Z, Xu Y, Wang Y, Xiao BK, Chen SM, Tao ZZ. Pharmacol. 2013 Jan 5;698(1-3):95-102. Indole-3-carbinol inhibits cell proliferation and induces apoptosis in Hep-2 laryngeal cancer cells. Oncol Rep. 2013 Jul;30(1):227-33. 100. Qi R, Feng M, Tan X, Gan L, Yan G, Sun C. FATP1 silence inhibits the differentiation and induces the apoptosis in chicken preadipocytes. Mol 121. Zhang S, Qi Y, Zhang H, He W, Zhou Q, Gui S, Wang Y. Melatonin Biol Rep. 2013 Apr;40(4):2907-14. inhibits cell growth and migration, but promotes apoptosis in gastric cancer cell line, SGC7901. Biotech Histochem. 2013 Aug;88(6):281-9. 101. Zeng J, Wang G, Li W, Zhang D, Chen X, Xin G, Jiang Z, Li K. Induction of cytopathic effect and cytokines in coxsackievirus B3-infected murine 122. Zheng M,Zheng Y, Xie L, Chang W, Gu N, Ji M. Orally Active Aurora astrocytes. Virol J. 2013 May 21;10:157. A/B Kinase Inhibitor, AM-005, Suppresses the Growth of Human Colon Carcinoma Cells. Drug Development Research. 2013 Jun;74(4):272-81. 102. Zheng W, Lu YB, Liang ST, Zhang QJ, Xu J, She ZG, Zhang ZQ, Yang RF, Mao BB, Xu Z, Li L, Hao DL, Lu J, Wei YS, Chen HZ, Liu DP. 123. Yue Y, Zhang P, Liu D, Yang JF, Nie C, Yang D. Hypoxia preconditioning SIRT1 mediates the protective function of Nkx2.5 during stress in enhances the viability of ADSCs to increase the survival rate of ischemic cardiomyocytes. Basic Res Cardiol. 2013 Jul;108(4):364. skin flaps inrats. Aesthetic Plast Surg. 2013 Feb;37(1):159-70. 103. Mo R, Sun Q, Li N, Zhang C. Intracellular delivery and antitumor effects 124. Zhang X, Zhang Y, Li Y. β-elemene decreases cell invasion by

4 / 5 C1011 Hoechst 33258 400-1683301/800-8283301 碧云天/Beyotime upregulating E-cadherin expression in MCF-7 human breast cancer cells. promotes programmed cell death and salicylic acid accumulation during Oncol Rep. 2013 Aug;30(2):745-50. the induced production of sesquiterpenes in cultured cell suspensions of 125. Ni Z, Dai X, Wang B, Ding W, Cheng P, Xu L, Lian J, He F. Natural Bcl-2 Aquilaria sinensis. Functional Plant Biology. 2014 Dec;42(4) 337-46. inhibitor (-)- gossypol induces protective autophagy via reactive oxygen 130. Wu YY, Zhou T, Wang Q, Dai PL, Xu SF, Jia HR, Wang X. Programmed species-high mobility group box 1 pathway in Burkitt lymphoma. Leuk Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Lymphoma. 2013 Oct;54(10):2263-8. Worker Brain Induced by Imidacloprid. J Econ Entomol. 2015 Aug; 126. Li X, Fan S, Li L, Wang L, Fan G, Zhao Q, Li Y. RNA interference- 108(4):1486-94. mediated knockdown of Livin suppresses cell proliferation and invasion 131. Xin YJ, Yuan B, Yu B, Wang YQ, Wu JJ, Zhou WH, Qiu Z. and enhances the chemosensitivity to cisplatin in human osteosarcoma Tet1-mediated DNA demethylation regulates neuronal cell death induced cells. Int J Oncol. 2013 Jul;43(1):159-68. by oxidative stress. Sci Rep. 2015 Jan 6;5:7645. 127. Wen X, Xu S, Liu H, Zhang Q, Liang H, Yang C, Wang H. Neurotoxicity 132. Ning H, Sun Z, Liu Y, Liu L, Hao L, Ye Y, Feng R, Li J, Li Y, Chu X, Li S, Induced by Bupivacaine via T-Type Calcium Channels in SH-SY5Y Cells. Sun C. Protects Hepatic Lipotoxicity by Regulating ER Stress PLoS One. 2013 May 2;8(5):e62942. through the PI3K/Akt/p53 InvolvedPathway Independently of Autophagy 128. Zhou DH, Wang X, Yang M, Shi X, Huang W, Feng Q. Combination of Inhibition. Nutrients. 2016 Apr 19;8(4):227. Low Concentration of (-)- (EGCG) and Curcumin 133. Li W, Tian YH, Liu Y, Wang Z, Tang S, Zhang J, Wang YP. Platycodin D Strongly Suppresses the Growth of Non-Small Cell Lung Cancer in Vitro exerts anti-tumor efficacy in H22 tumor-bearing mice via improving and in Vivo through Causing Cell Cycle Arrest. Int J Mol Sci. 2013 Jun 5; immune function andinducing apoptosis. J Toxicol Sci. 2016; 41(3): 14(6):12023-36. 417-28. 129. Juan Liu, Yanhong Xu, Zheng Zhang and Jianhe Wei. Hydrogen peroxide Version 2016.08.02

碧云天/Beyotime 400-1683301/800-8283301 C1011 Hoechst 33258 5 / 5