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Genetic Diversity of Brassica Carinata with Emphasis on the Interspecific

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Genetic Diversity of Brassica Carinata with Emphasis on the Interspecific Plant Breeding 126, 487—491 (2007) doi:10.1111/j.1439-0523.2007.01393.x Ó 2007 The Authors Journal compilation Ó 2007 Blackwell Verlag, Berlin Genetic diversity of Brassica carinata with emphasis on the interspecific crossability with B. rapa Y. Jiang1,2, E. Tian1 , R. Li1 , L. Chen1 and J. Meng1 1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2Present address: Institute of Crop Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China; 3Corresponding author, E-mail: [email protected] With 3 figures and 1 table Received 23 March, 2006/Accepted 9 February, 2007 Communicated by A.-M. Chevre Abstract strated that a hexaploid hybrid (AABBCC), derived from an Brassica carinata (BBCC, 2n ¼ 34) possesses many good agronomic interspecific cross between B. carinata and B. rapa (AA, 2n ¼ characters and, to provide more genetic information about this species 20), could be used as a bridge hybrid (Li et al. 2004). By and utilize it further, 110 accessions of B. carinata were tested for crossing the hexaploid hybrid with B. napus,itwaspossibleto genetic variation by using 233 amplified fragment length polymorph- create a pentaploid hybrid (AABCC). This pentaploid hybrid ism markers, so that a dendrogram could be constructed. To combine wasthenusedtocreateavariantofB. napus whose DNA the good traits of B. carinata and B. rapa (AA, 2n ¼ 20) with those of comprised half the A genome of B. rapa and half the B. napus (AACC, 2n ¼ 38) which is the major rapeseed variety in CgenomeofB. carinata (Li et al. 2004, 2006). The crossability China, interspecific crosses between these two species have been made between B. carinata and the three basic species of Brassica and which have resulted in 276 doubled hybrids (AABBCC). These B. napus is very low (Stebbins 1958). Several interspecific hexaploids combine positive characters and can be used for crossing with B. napus. Brassica carinata as the female parents exhibited crosses involving B. carinata have been made, which have extensive differences in interspecific crossability and 20 high crossabil- resulted in the transfer of some desirable traits from B. carinata ity accessions were identified. The Poisson Regression Model analysis to other Brassica species (Choudhary et al. 2000, Rahman result (P < 0.0255) indicated that such differences were due to the 2001, Tonguc and Griffiths 2004). Therefore, exploring geno- genotype of the accessions. Accessions with high crossability could types of B. carinata that have high interspecific crossability promote gene flow within the genus Brassica. would be useful in order to promote gene flow within the genus Brassica. Key words: Brassica carinata — Brassica napus — genetic This investigation has three objectives. First, to evaluate the variation — amplified fragment length polymorphism — genetic diversity of 110 accessions of B. carinata. Secondly, to interspecific cross — gene pool create a new bridge germplasm (AABBCC) for transferring genetic material from B. carinata and B. rapa to B. napus. Ethiopian mustard (also called Abyssinian Mustard or Abys- Thirdly, to screen genotypes of B. carinata for high crossability sinian Cabbage Brassica carinata, BBCC, 2n ¼ 34) possesses to B. rapa. many desirable agronomic characteristics that are rare in the other Brassica oil crops: heat and drought tolerance, disease and pest resistance, and a yellow-seeded germplasm (Gugel Materials and Methods et al. 1990, Bayeh and Gebre Medhin 1992, Yitbarek 1992). Its Plant materials: One hundred and seven accessions of Brassica. importance to breeders has increased because of rapid carinata, mainly of Ethiopian origin, were obtained from the improvement in its seed quality (Getinet et al. 1994, Velasco Centre for Genetic Resources, Wageningen, the Netherlands et al. 2003, Teklewold and Becker 2005). As a result, its genetic (CGN; Supplementary Table 1). An additional three accessions diversity has prompted considerable interest among Brassica (88-216845, 80-139, 88-219790) of B. carinata were obtained from breeders. Alemayehu and Becker (2002) analysed 13 morpho- Germany (Supplementary Table 1). Twenty-nine accessions of logical and seed quality characteristics of 36 accessions of B. rapa were collected, mainly from various locations in China B. carinata and found extensive genetic variability. Warwick and grown for at least four generations in Wuhan, China (Supplementary Table 2). et al. (2006) used amplified fragment length polymorphism (AFLP) to evaluate the patterns and extent of genetic diversity Field trial: All accessions were sown in the field station of the of B. carinata. Exploring the genetic diversity of B. carinata Huazhong Agricultural University, Wuhan in September 2003. Each may provide more information for plant breeders. accession was planted in three rows with 10 plants per row. To ensure Interspecific hybridization is a useful way to transfer pollination by B. rapa throughout the flowering period of B. carinata, desirable traits from one species to another, and has enriched the seeds of B. rapa were sown 43 days and 55 days after sowing the the gene pool of Brassica crops (Allard 1960, Prakash and accessions of B. carinata, respectively. When the plants flowered in Chopra 1998, Liu 2000). Over the past few decades, numerous March 2004, buds from three or four plants of each B. carinata efforts to widen the genetic basis of B. napus (AACC, 2n ¼ 38) accession were picked and randomly fertilized with pollen of B. rapa. which is narrow when compared to B. rapa and B. carinata At harvest, the crossability of different parents was analysed. In 2005, have been undertaken. In previous research, it was demon- four accessions (CGN03941, CGN03949, CGN03995 and CGN04004) www.blackwell-synergy.com 488 Jiang, Tian, Li, Chen and Meng of B. carinata with high interspecific crossability and one accession AFLP procedure: DNA samples were prepared from young leaves (CGN03931) with low crossability to B. rapa were selected for crossing of five plants of each accession of B. carinata to estimate the with B. rapa to assess the environmental effect on the interspecific genetic variation amongst accessions. DNA was extracted using a crossability. To make the triploid interspecific hybrids fertile, the modified Cetyltrimethyl Ammonium Bromide (CTAB) procedure putative hybrids were treated with 0.1% (w/v) colchicine. (Doyle and Doyle 1990), concentration and purity were measured Fig. 1: Dendrogram generated with 233 amplified fragment length polymorphism markers on 110 accessions of Brassica carinata. The scale shows SM similarity coefficients and the dendrogram was developed using the Unweighted Pair Group Method with Arithmetic mean clustering procedure Genetic diversity of Brassica carinata 489 by a Beckman spectrophotometer (Beckman Coulter Inc., Fuller- Interspecific crossability ton, CA, USA). AFLP–PCR was performed according to the In total, 409 interspecific combinations were made. Eventually, method described by Vos et al. (1995), with minor modifications. 13 000 seeds were obtained from 40,000 cross-pollinated buds Genomic DNA (200 ng) was restricted with EcoR1 (2 U/200 ng DNA) and Mse1 (2 U/200 ng DNA) for 5 h. After ligation for involving 375 combinations. As in most cases B. carinata and 10 h (1 U ligase/200 ng DNA), the preamplification was carried B. rapa could be distinguished easily by their morphology, it out in a Perkin-Elmer 9600 thermocycler (Foster City, CA, USA). was not difficult to identify true hybrid plants. Few of the The pre-PCR products were diluted 1 : 20 with deionized water hybrids required verification by molecular marker analysis and and used for a second PCR with selective primers. The PCR chromosome counting because they resembled their maternal products were separated on a 6% polyacrylamide gel followed by parents. Eventually, more than 5000 hybrid plants were silver staining. generated from 326 interspecific combinations (Supplementary Table 1). However, colchicine treatment doubled the number TM Data analysis: To facilitate scoring across gels, a GeneRuler 50 bp of chromosomes in only 23.3% of the hybrid plants, which AFLP DNA ladder (MBI Fermentas, GmbH, Germany) was used. resulted in 276 combinations of hexaploid hybrid plants. Polymorphic fragments between 50 and 400 bp in size were scored as The ICIs of accessions of B. carinata and B. rapa differed either present (1) or absent (0). DNA markers were analysed by the considerably (Fig. 2). Some accessions of B. carinata yielded Unweighted Pair Group Method with Arithmetic mean. A dendro- more than 80 hybrid seeds per 100 interspecific pollinated buds, gram was constructed by software NTSYS-PC 2.1 (Rohlf 2002) using SM coefficients (Sokal and Michener 1958). The SM coefficient was whereas some produced none. A set of data selected from the calculated with the formula: random interspecific combinations met the requirement of PRM where the male parent, of B. rapa, ÔBaijian 13Õ, was crossed to SMij ¼ðn10 þ n01Þ=ðn11 þ n10 þ n01 þ n00Þ; nine female parents each involving three or four individual plants. The Value/DF of Deviance and Pearson chi-square were where n was the number of bands in common between accessions 11 0.21 and 0.16, respectively, which indicated that PRM was fitting i and j,n was the number of bands present in i and absent in j,n 10 01 the data set for the analysis. The PRM analysis result (v2 ¼ was the number of bands absent in i and present in j, and n00 was the number of bands both absent in i and j. 17.48, df ¼8, P < 0.0255) showed that the ICI for each female Bootstrap analysis was performed with the Phylogenetic Analysis, parent was statistically significant, indicating that such differ- using Parsimony (PAUP) software (Swofford 1999) to evaluate the ences were caused by the genotype of the accessions. robustness of the nodes of the dendrogram. 90 80 Crossability analysis: The interspecific crossability index (ICI) 70 between B. carinata and B. rapa was defined as the number of 60 hybrid plants obtained from 100 cross-pollinated buds, an ICI value 50 40 bigger than 20 being considered an accession with high crossability.
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