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Heated Nebulizer Microchips for Mass Spectrometry

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Heated Nebulizer Microchips for Mass Spectrometry Recent Publications in this Series: for Mass Spectrometry Heated Nebulizer Microchips MARKUS HAAPALA 31/2009 Timo Sipilä Plasmids and Aromatic Degradation in Sphingomonas for Bioremediation - Aromatic Ring Cleavage Genes in Soil and Rhizosphere 32/2009 Jack C. Leo Structural and Functional Studies on Trimeric Autotransporters 33/2009 Päivi Uutela Liquid Chromatography-Tandem Mass Spectrometry in Studies of Neurotransmitters and Their Metabolites in the Brain 34/2009 Ranad Shaheen Bacillus Cereus Spores and Cereulide in Food-Borne Illness 35/2009 Katri Berg Heterotrophic Bacteria Associated with Cyanobacteria in Recreational and Drinking Water 36/2009 Aneta Skwarek-Maruszewska Actin Dynamics in Muscle Cells 37/2009 Laura Riihimäki-Lampén Interactions of Natural Products with β-Lactoglobulins, Members of the Lipocalin Family 38/2009 Miia R. Mäkelä The White-Rot Fungi Phlebia radiata and Dichomitus squalens in Wood-Based Cultures: Expression of Laccases, Lignin Peroxidases, and Oxalate Decarboxylase 39/2009 Esko Oksanen Enzyme Molecular Choreography - Studies of Soluble Inorganic Pyrophosphatases 40/2009 Maxim M. Bespalov GDNF Receptors: Veterans and Novices 1/2010 Taina Sten Stereoselectivity of the Human UDP-glucuronosyltransferases (UGTs); Studies on Androgens and Propranolol Glucuronidation 2/2010 Marika Suomalainen Fine-Tuning of The Signalling Network Controlling Morphogenesis and Stem Cell Development in Teeth Heated Nebulizer Microchips 3/2010 Kimmo Rantalainen Biochemical and Structural Properties of Potato virus A VPg for Mass Spectrometry 4/2010 Sanna Kaivosaari N-Glucuronidation of Drugs and Other Xenobiotics 5/2010 Johanna Mantela Role of Cell Cycle Regulators in Development of The Inner Ear 6/2010 Pinja Jaspers Stress and Developmental Responses in Arabidopsis Thaliana: Regulation Through The Transcription Factor –Interacting Protein RCD1 7/2010 Marika Pohjanoksa-Mäntylä Medicines Information Sources and Services for Consumers: A Special Focus on the Internet and People with Depression MARKUS HAAPALA 8/2010 Satu Lakio Towards Real-Time Understanding of Processes in Pharmaceutical Powder Technology 9/2010 Manu Eeva Division of Pharmaceutical Chemistry Plant Secondary Metabolites in Peucedanum palustre and Angelica archangelica and Their Plant Cell Cultures Faculty of Pharmacy 10/2010 Niina Kivikero Granulation in Miniaturised Fluid Bed Using Electrostatic Atomisation University of Helsinki 11/2010 Tiina Heikkilä Miniaturization of Drug Solubility and Dissolution Testings Helsinki 2010 ISSN 1795-7079 ISBN 978-952-10-6257-5 Dissertationes bioscientiarum molecularium Universitatis Helsingiensis in Viikki 12/2010 12/2010 Division of Pharmaceutical Chemistry Faculty of Pharmacy University of Helsinki Finland Heated Nebulizer Microchips for Mass Spectrometry by Markus Haapala ACADEMIC DISSERTATION To be presented, with the permission of the Faculty of Pharmacy of the University of Helsinki, for public examination in Auditorium 1041, Viikki Biocenter 2 (Viikinkaari 5), on May 21st, 2010, at 12 noon. Helsinki 2010 Supervisors: Professor Risto Kostiainen Division of Pharmaceutical Chemistry, Faculty of Pharmacy University of Helsinki Finland Professor Tapio Kotiaho Division of Pharmaceutical Chemistry, Faculty of Pharmacy and Laboratory of Analytical Chemistry, Department of Chemistry, Faculty of Science University of Helsinki Finland Reviewers: Professor Janne Jänis Department of Chemistry, Faculty of Science and Forestry University of Eastern Finland Finland Professor Elisabeth Verpoorte Department of Pharmacy, Faculty of Mathematics and Natural Sciences University of Groningen The Netherlands Opponent: Professor Frants Lauritsen Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences University of Copenhagen Denmark © Markus Haapala 2010 ISBN 978-952-10-6257-5 (paperback) ISBN 978-952-10-6258-2 (PDF) ISSN 1795-7079 http://ethesis.helsinki.fi Helsinki University Print Helsinki 2010 CONTENTS PREFACE ................................................................................................................................................ 5 ABSTRACT.............................................................................................................................................. 6 LIST OF ORIGINAL PUBLICATIONS............................................................................................... 8 ABBREVIATIONS................................................................................................................................ 10 1 INTRODUCTION .............................................................................................................................. 12 1.1 MINIATURIZATION OF ANALYTICAL DEVICES................................................................................. 12 1.1.1 Materials for microfabrication.............................................................................................. 13 1.1.2 Methods for microfabrication................................................................................................ 14 1.2. ATMOSPHERIC PRESSURE IONIZATION SOURCES AND THEIR MINIATURIZED VERSIONS.................. 15 1.2.1 Electrospray ionization ......................................................................................................... 16 1.2.2 Sonic spray ionization ........................................................................................................... 19 1.2.3 Atmospheric pressure chemical ionization............................................................................ 19 1.2.4 Atmospheric pressure photoionization .................................................................................. 21 1.2.5 Heated nebulizer microchips................................................................................................. 22 1.3 MINIATURIZATION OF CHROMATOGRAPHY .................................................................................... 25 1.4 AMBIENT MASS SPECTROMETRY .................................................................................................... 26 1.5 TEMPERATURE AND FLUIDIC MEASUREMENTS IN MICROSCALE ...................................................... 27 2 AIMS OF THE STUDY...................................................................................................................... 30 3 MATERIALS AND METHODS ....................................................................................................... 31 3.1 CHEMICALS AND MATERIALS ......................................................................................................... 31 3.2 INSTRUMENTATION ........................................................................................................................ 33 3.3 MICROCHIP FABRICATION .............................................................................................................. 36 3.3.1 Silicon–glass heated nebulizer microchips............................................................................ 36 3.3.2 All-glass heated nebulizer microchips................................................................................... 37 3.3.3 Integrated liquid chromatography – heated nebulizer microchips........................................ 38 3.4 EXPERIMENTAL SETUPS.................................................................................................................. 39 3.4.1 Thermal and fluidic measurements........................................................................................ 39 3.4.2 Chromatography and mass spectrometry.............................................................................. 41 4 RESULTS AND DISCUSSION ......................................................................................................... 44 4.1 THERMAL AND FLUIDIC PROPERTIES OF HEATED NEBULIZER MICROCHIPS ..................................... 44 4.1.1 Performance of the thermocouple sensor.............................................................................. 45 4.1.2 Thermal and fluidic characterization of heated nebulizer microchips.................................. 45 4.2 DIRECT INFUSION STUDIES ............................................................................................................. 50 4.2.1 Microchip atmospheric pressure photoionization – Fourier transform ion cyclotron resonance mass spectrometry................................................................................................ 51 4.2.2 Microchip sonic spray ionization ..........................................................................................55 4.3 CONNECTING HEATED NEBULIZER MICROCHIPS WITH CHROMATOGRAPHY .................................... 56 4.3.1 Gas chromatography – microchip atmospheric pressure photoionization – mass spectrometry ..........................................................................................................................57 4.3.2 Capillary liquid chromatography – microchip atmospheric pressure photoionization – mass spectrometry .................................................................................................................59 4.4 INTEGRATION OF CHROMATOGRAPHIC SEPARATION AND HEATED NEBULIZER ...............................61 4.4.1 Microchip structure and operation........................................................................................61 4.4.2. Liquid chromatography ........................................................................................................63 4.5 DESORPTION
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