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Photosynthetic Bacteria for Bioremediation of Hydrogen Sulphide in Aquatic Systems

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Photosynthetic Bacteria for Bioremediation of Hydrogen Sulphide in Aquatic Systems PHOTOSYNTHETIC BACTERIA FOR BIOREMEDIATION OF HYDROGEN SULPHIDE IN AQUATIC SYSTEMS Thesis Submitted to the COCHIN UNIVERSITY OF SCIENCE AND TECHNOLOGY In partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in ENVIRONMENTAL MICROBIOLOGY AND BIOTECHNOLOGY under the Faculty of Environmental Studies by AMMU THOMAS (Reg. No.3471) NATIONAL CENTRE FOR AQUATIC ANIMAL HEALTH COCHIN UNIVERSITY OF SCIENCE AND TECHNOLOGY KOCHI 682016, KERALA, INDIA August 2017 Photosynthetic Bacteria for Bioremediation of Hydrogen Sulphide in Aquatic Systems Ph.D. Thesis under the Faculty of Environmental Studies Ammu Thomas Research Scholar National Centre for Aquatic Animal Health School of Environmental Studies Cochin University of Science and Technology Kerala, India Supervising Guide Prof. I. S. Bright Singh UGC BSR Faculty National Centre for Aquatic Animal Health Cochin University of Science and Technology Kerala, India. National Centre for Aquatic Animal Health Cochin University of Science and Technology Kochi – 682016, Kerala, India August 2017. This is to certify that research work presented in the thesis entitled is “PHOTOSYNTHETIC BACTERIA FOR BIOREMEDIATION OF HYDROGEN SULPHIDE IN AQUATIC SYSTEMS” based on the original work done by Mrs. Ammu Thomas under my guidance at National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin- 682016, in partial fulfillment of the requirements for the Degree of Doctor of Philosophy and that no part of this work has previously formed the basis for the award of any degree, diploma, associateship, fellowship or any other similar title or recognition. All the relevant corrections and modifications suggested by the audience and recommended by the doctoral committee of the candidate during the pre-synopsis seminar have been incorporated in the thesis. Cochin-682016 Prof. I. S. Bright Singh August 2017 (Supervising Guide) UGC BSR Faculty National Centre for Aquatic Animal Health Cochin University of Science and Technology I hereby do declare that the work presented in this thesis entitled “PHOTOSYNTHETIC BACTERIA FOR BIOREMEDIATION OF HYDROGEN SULPHIDE IN AQUATIC SYSTEMS” is based on the original work done by me under the guidance of Prof. I. S. Bright Singh, UGC BSR Faculty, National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin- 682016, in partial fulfillment of the requirements for the Degree of Doctor of Philosophy and that no part of this work has previously formed the basis for the award of any degree, diploma, associateship, fellowship or any other similar title or recognition. Cochin-682016 Ammu Thomas August 2017 First and above all, I praise God, the Almighty for providing me this opportunity and granting me the capability to proceed successfully. This thesis appears in its current form due to the assistance and guidance from several people and without their help this would not have been accomplished successfully. I would therefore like to express my sincere thanks to all of them. I express my profound sense of reverence to my guide and promoter Prof. I.S. Bright Singh, UGC BSR Faculty, National Centre for Aquatic Animal Health, Cochin University of Science and Technology, for his constant guidance, support, motivation and untiring help during the course of my PhD. His in-depth knowledge on a broad spectrum of life science especially in Microbial Biotechnology topics has been extremely beneficial for me. He has given me all the support and freedom during my research and he has always been nice to me. I will always remember his calm and patient nature, his unflinching courage and conviction will always inspire me, I am thankful to the Almighty for giving me a mentor like him. I would like to acknowledge my sincere gratitude to Dr.A Mohandas for his kind support, motivation and numerous discussions on related topics that helped me improve my knowledge in the area. The funny moments, especially during my pregnancy time, support he gave and the courage throughout the time with him made the years so joyful. I would like to thank to Dr. Rosamma Philip for her friendly support and inspirations. Her valuable suggestions, approachability and advices helped me to proceed with my tasks successfully. I sincerely state my thanks to Dr.Rajathy, the director, School of Environmental Studies, for facilitating me as a scholar and for all the support rendered throughout the period. I take this opportunity to acknowledge Dr.Valsamma Joseph, Director, National Centre for Aquatic Animal Health. She always gave care, love and support throughout the time. Her energy, pleasant attitude and enthusiasm inspired me to face any challenges. I am equally thankful to Dr. Sajeevan. T.P, Associate Professor, NCAAH. I am grateful to both of them for their intellectual contributions and friendly approach. I am greatly thankful to Dr.Ammini Joseph, Dr. E.P.Yesodharan, Dr.Suguna Yesodharan, Dr.V.Harindranathan Nair, Dr.Sivanandan Achari and Mr.M.Anand and all the other faculty members, School of Environmental studies for their help and support. All the teachers and staffs provided their seamless support throughout the period of my PhD programme. I am also thankful to Dr.Swapna.P.Antony and Ms.Limmy,T.P for their whole hearted support and timely suggestions. I wholeheartedly thank the library staff of School of Environment studies and Marine Sciences, Cochin University of Science and Technology for all the support and help given. I am thankful to them for teaching me to use the facilities provided by University Grants Commission. The road to my Ph.D. started with training for one year at National Centre for Aquatic Animal Health, CUSAT. I take this opportunity to say heartfelt thanks to Dr. Dhamodharan sir as well. He introduced me to Dr. Bright Sigh and provided excellent training in the field of Microbiology under the guidance of Dr. Manju N, J and Dr Deepesh Valsan. During the period, both of them gave sisterly affection to me and helped me to learn and enhance my knowledge in the field of Microbiology. I take this opportunity to thank both of them and let God the Almighty shall grant all their wishes to be fruitful. It has been a great privilege to be a part of National Centre for Aquatic Animal Health for my research work. It is my pleasant duty to thank all the fellow researchers at the centre. Especially I would like to mention the seniors, Dr.Sreedharan, Dr.Rejesh kumar, Dr.Sudheer.N.S, Dr.Ranjit Kanjoor, Dr.Seena Jose, Dr. Jiji Poulose, Dr. B. Sreelekshmi, Dr.Rose Mary Jose, Dr.Prem Gopinath, Dr.Sareen Sarah John, Dr.Arun Augustine, Dr. Deepa.S. Nair and Dr.Rojith G for all the wonderful help rendered. I would like to thank Dr.Jayesh Puthumana and Dr.Vrinda Sukumaren for the constant encouragement, valuable suggestions and continuous feedback. Without their support I could not make this a success and thanks a lot to both of them. I express my sincere thanks to my colleagues Archana Nair and Dr. Preetha who helped me a lot and gave me remarkable support and encouragement during my time here. I take this opportunity to thank my best friends Dr.Haseeb (Ekka) and Dr.Riya George. They both gave a lot of encouragement and their deep knowledge in the subject areas was always a helping hand. They were always supportive in my personal life as well and their advices and help made my life more cheerful at the Research centre and of course I really thank their family members also for their love and support. It gives me immense pleasure in acknowledging my heartfelt thanks to Dr.Priyaja P, Ms. Ramya.R.Nair and Dr. Jasmin Anas for their instructions and help in Molecular Biology works. I am extremely thankful to my colleague, Dr.Preena for her constant support, prayers and encouragement throughout the time. Mere words of thanks will be inadequate for the unconditional love, prayers and help rendered by my sweet friends, Dr.Sunitha Poulose, Dr.Surekha Mol. I.S, and Ms.Deepa G.D. My thesis would never have come to completion if I were not endowed with their constant support, care, encouragement and compassion. I deeply appreciate the extreme sincerity and suggestions rendered by my beloved brothers Boobal. R and Linu Balan for their meticulous reviews and feedbacks. I really enjoyed the cheerful moments with them throughout the period. I cherish the joyful moments with my „nearest‟ and dearest colleagues, Ms.Sanyo Sabu and Ms. Merlin T.S and Deepa. It was a great time that will be always in my memory sharing everyone‟s happiness and sorrows together as a family. I wish to extend my warmest thanks to all the past and present Post-Doctoral Fellows, Dr.Goplakrishnan, Dr. Sabu, Dr.Sijo, Dr.Divya, and Dr.Shalini.K for their priceless encouragement. I owe my deepest gratitude to all other colleagues and juniors at NCAAH for providing me a pleasant atmosphere throughout the period. My loving thanks to Ms.Soumya, Ms.Dhaneesha, Ms.Lakshmi, Ms.Vinu Sree, Ms.Asha, Ms.Jisha Kumaran, Mr.Anoop.B.S, Mr.Devassy Christo, Ms.Ajitha Ms.Krishna Priya, Mrs.Indira Mr.Vinod and Mr.Vipin Mohanan for being with me with all the support and encouragement. The technical staff at NCAAH Ms.Blessy Jose, Amja (former staff) and Ms.Surya Sugunan helped me a lot in following my dream and I acknowledge my thanks to them for the sincere help, support, lovely and homely talks. I wish to thank Ms.Parisachechi, Kusumam Chechi and Soman chettan for their love and affection and I always enjoyed our “Lunch time” with the homely and mouth-watering food brought by chechi and chettan. The sweet memories will always be carried forward throughout my life. I use this opportunity to acknowledge thanks to all the batches of M.Tech Marine Biotechnology students.
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