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Te Mentor Mauroraima USTE 20180084776A1MENTOR MAURORAIMA (19 ) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2018 / 0084776 A1 HURST et al. ( 43 ) Pub . Date : Mar . 29, 2018 (54 ) AGRICULTURAL COMPOSITION Publication Classification (51 ) Int. Ci. (71 ) Applicant : Agresearch Limited , Hamilton (NZ ) AOIN 25 / 12 (2006 .01 ) AOIN 25 /00 (2006 .01 ) (72 ) Inventors : Mark Robin Holmes HURST , AOIN 63 /04 ( 2006 . 01 ) (52 ) U . S . CI. Hamilton (NZ ) ; Jayanthi CPC . .. .. A01N 25 / 12 ( 2013 .01 ) ; AOIN 63 /04 SWAMINATHAN , Hamilton (NZ ) (2013 . 01 ) ; AOIN 25 /006 ( 2013. 01 ) ( 73 ) Assignee : Agresearch Limited , Hamilton (NZ ) ( 57 ) ABSTRACT A granular composition that comprises one or more micro organisms, two or more plant powders and a biodegradable ( 21 ) Appl. No. : 15 /566 ,156 water - absorbent agent. Also provided is a method of pro ducing a granular composition by admixing a composition (22 ) PCT Filed : Apr. 13 , 2016 comprising two or more plant powders and a biodegradable water -absorbent agent, and ( i ) one or more lubricants to ( 86 ) PCT No. : PCT/ NZ2016 /050059 form a dough , and processing the dough to form granules , or ( ii ) coating a substrate with the dough to form the granular $ 371 ( c ) ( 1 ) , composition . The microorganism can be located in the ( 2 ) Date : Oct. 12 , 2017 admixture , the dough or both the admixture and the dough . The granular composition is applied to soil to , for example , ( 30 ) Foreign Application Priority Data kill or control pests , maintain or increase plant or crop growth , maintain or increase pasture production , maintain or Apr. 13 , 2015 (NZ ) .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 706932 increase plant, crop or pasture yield , deliver probiotics to Feb . 9 , 2016 (NZ ) .. 716740 grazing animals or livestock , and /or bioremediate the soil. Patent Application Publication Mar . 29 , 2018 Sheet 1 of 6 US 2018 / 0084776 A1 1 . 00E + 11 1 . 00E + 10 1 .00E + 09 1 . 00E + 08 1 . 00E + 07 viablecellcount(cfu/g) 1 . 00E + 06 - - - - . 1 . 00E + 05 1 . 00E + 04 1 . 00E + 03 1 . 00E + O2 1 . 00E + 01 1 . 00E + 00 . 20 40 60 80 100 Storage time ( days ) aw T0 0 .6172 I 3W TO : 0 . 6981 + aW TO : 0 .5449 aw TO : 0 .6452 - * W TO = 0 .5443 Figure 1 00012 M 11 Mmmm. S 008410 GrowthofSerratiaentomophila(CFU) 0060 * * * * 10369 * * * 1. 596408 O ** ** 30694 .- .- TWE08 wwwwwwwwwwwwwwwwww 0 20 40 60 80 100 120 08 - retmeni Fies ) Figure 2 Patent Application Publication Mar . 29 , 2018 Sheet 2 of 6 US 2018 / 0084776 A1 LO0E412. 5100011 100 * 10 annnnnnnnnnn .00E 09 GrowthofYersiniaentomoplaga(CFU) 1 . 00E+ 08 ** * 1. 72E +07 he 1 . 65€ 07 . * * * * * * 3 $ 06 - 05 * * * * * * * * * * * ** * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * 1. 00E + 08 . : : , , LLLLLLLLL : . 1. 00E + 08 Pont treatment time this ) Figure 3 1 71 999999YYYYYYYYYYYYYYYYYYYYYYYYYYYYY -. GrowthofSerratiaprotecranularis(CFU) . - * * * * * * 733EOS www * 8 .906405 - segitigarea 2 .78 20T . 0 20 40 60 80 100 120 Pastireatment time this Figure 4 Patent Application Publication Mar . 29 , 2018 Sheet 3 of 6 US 2018 /0084776 A1 ? 100kV UROL W2133min SE 100V % X30 journ WO 12 am SE ! 10 .0KV X300 Or) WD 12 . 750 SE ! 100 X30 ) 10 : 07 WD 12. 6emir D SE ! 10. 05V X300 Jum wo som E SH K DV * * *XX 10 ; 6 * WO 12 .Stork SET 10 . 0KW QXW 2 & aun , SB 109V X300 10 , 02WO 12. BiG . Figure 5 Patent Application Publication Mar, 29 , 2018 Sheet 4 of 6 US 2018/ 0084776 A1 S ? 1?4 LPLITS :? { { }t { S 95 { L} { S Control-Ye Yeblinding sand Crntro {-Vet lillYeExtruded granule Yewet granulation MetZeolite Granule MetZeolitelow Granule HighMet Granule LowMet Granule High Figure 6 Patent Application Publication Mar . 29 , 2018 Sheet 5 of 6 US 2018 / 0084776 A1 e ? - - - Control - Ye Percentagemortality - – Ye Extruded granule Ye wet granulation a . Ye blinding sand - 10 ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ * * wy Day Day 4 Day 6 Day 7 Day 8 Day 14 Day 21 Day 28 Day 35 Day 47 . § w Control - Met Percentagemortality Met Zeolite Granule - Met Granule Low * * • • Met Granule High - - . P . - - - - -10 111111 Day o Day 4 Day 6 Day 7 Day 8 Day 14 Day 21 Day 28 Day 35 Day 47 Figure 7 Patent Application Publication Mar . 29 , 2018 Sheet 6 of 6 US 2018 / 0084776 A1 helstsowewe -- - Ye Extruded - moistened . -. - Ye Extruded Percentagemortality R88908909 - - - Ye Wet Granulation mammor S.. .. .. .. — Ye Blinding sand - . - Carrot only controls . - - - - - 10 cm unny requenos 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Day Figure 8 US 2018 / 0084776 A1 Mar . 29 , 2018 AGRICULTURAL COMPOSITION [ 0017 ] coating the dough with an exterior coating com prising one or more microorganisms to form the granular FIELD OF THE INVENTION composition . [0001 ] The present invention relates to an agricultural [0018 ] In a further aspect the invention relates to a method composition comprising one or more microorganisms, its of delivering one or more microorganisms to soil , compris methods of preparation and its application to soil . ing applying the granular composition of the invention on to soil . [0019 ] In a further aspect, the invention provides a method BACKGROUND TO THE INVENTION of controlling insect pests , the method comprising placing [ 0002 ] Microorganisms such as bacteria and fungi are insecticidal granular compositions in proximity to an insect useful alternatives to chemical agents for the improvement pest, the granular compositions being as claimed above . and / or maintenance of soil and plant health , including the [0020 ] Any one or more of the following embodiments control of pests . may relate to any of the aspects described herein or any [ 0003 ] The utility of microorganisms for application to combination thereof. soil is limited by the sensitivity of such microorganisms to [0021 ] In various embodiments the granular composition environmental conditions after application and during stor has age of compositions comprising microorganisms. [ 0022 ] a ) a bulk density of from about 0 . 2 g / cm² to [ 0004 ] Compositions that adequately protect the microor about 1 . 5 g / cm " , or ganism from environmental exposure are costly to manu [0023 ] b ) a mean diameter ( weight - based ) of from about facture . 1 mm to 5 mm , or 10005 ] It is an object of the present invention to provide an [ 0024 ] c ) a particle size distribution whereby at least agricultural composition comprising one or more microor 90 % of the granular composition have a diameter of ganisms, or to at least provide the public with a useful from 1 mm to 5 mm , or choice . 10025 ] d ) a particle size distribution whereby at least 90 % of the granular composition has a diameter within SUMMARY OF THE INVENTION 1 mm of the mean diameter , or [0006 ] In a first aspect, the invention provides a granular [0026 ] e ) a water activity of from about 0 . 2 to about composition comprising one or more microorganisms, two 0 . 95 , or or more plant powders and a biodegradable water- absorbent 100271 f ) a hardness ( the maximum load to crush ) of agent. from about 10 N to about 100 N , or [0007 ] In a further aspect, the invention provides a granu [0028 ] g ) a brittleness of from about 10 N to about 100 lar composition comprising one or more microorganisms N , or having a loading of less than 101° cells per gram of granular [0029 ] h ) a porosity of from about 1 to about 1000 , or composition , two or more plant powders and a biodegrad [ 0030 ] i ) a swellability of from about 50 to about 300 % , able water- absorbent agent capable of absorbing at least or 10 % of its dry weight in water . [ 0031 ] j ) any combination of two or more of ( a ) to ( i ) [ 0008 ] In a further aspect, the invention provides a granu above . lar composition comprising one or more bacteria , two or [0032 ] In some embodiments the granular composition more plant powders and a biodegradable water- absorbent has a bacterial loading of less than about 1011 , 1010 , 10 % , 108 , agent capable of absorbing at least 10 % of its dry weight in 107, 106 , 10 % or 104 cells per gram of granular composition , water . and useful ranges may be selected between any of these [0009 ] In a further aspect the invention relates to a method values . of producing a granular composition , the method comprising [0033 ] In various embodiments said two or more plant [0010 ] admixing a composition comprising one or more powders comprises a bran as at least one of said two or more microorganisms, two or more plant powders , a biodegrad plant powders . able water- absorbent agent capable of absorbing at least [0034 ] In various embodiments the granular composition 10 % of its dry weight in water, and one or more lubricants comprises a phagostimulant. to form a dough , and [0035 ] In various embodiments the granular composition [0011 ] processing the dough to form the granular compo comprises one or more polysaccharides . sition . [0036 ] In various embodiments the granular composition [ 0012 ] In a further aspect the invention relates to a method comprises one or more CO2- generating agents . of producing a granular composition , the method comprising 10037 ] In various embodiments the granular composition [0013 ] admixing a composition comprising one or more comprises at least about 1x104, 1x105 or 1x10 cfu / g after microorganisms, two or more plant powders and a biode storage at 4° C . for 14 days, and useful ranges may be gradable water - absorbent agent capable of absorbing at least selected between any of these values . 10 % of its dry weight in water , to form a dough , and [0038 ]. In various embodiments the granular composition [ 0014 ] coating a substrate with the dough to form the retains its integrity for at least about 48 hours after contact granular composition . ing about 0 . 1 , 0 . 2 , 0 . 3 , 0 . 4 , 0 . 5 , 0 . 6 , 0 . 7 , 0 . 8 , 0 . 9 or 1 ml of [ 0015 ] In a further aspect the invention relates to a method water per gram of granular composition .
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