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(12) United States Patent (10) Patent No.: US 9,364,003 B2 Prosch Et Al

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(12) United States Patent (10) Patent No.: US 9,364,003 B2 Prosch Et Al US009364003B2 (12) United States Patent (10) Patent No.: US 9,364,003 B2 Prosch et al. (45) Date of Patent: Jun. 14, 2016 (54) MITIGATING NECROSS IN TRANSGENIC (56) References Cited GLYPHOSATE-TOLERANT COTTON PLANTS TREATED WITH HERBICIDAL U.S. PATENT DOCUMENTS GLYPHOSATE FORMULATIONS 3,455,675 A 7, 1969 Irani 3,556,762 A 1, 1971 Ham (75) Inventors: Stephen D. Prosch, Ballwin, MO (US); 3,853,530 A 12, 1974 Franz Michael E. Seitz, Dublin, CA (US); 3.969,398 A 7, 1976 Hershman David R. Eaton, Kirkwood, MO (US); 4,237,065. A 12, 1980 Ehrat 4,397,676 A 8, 1983 Bakel David Z. Becher, St. Louis, MO (US) 4.486.359 A 12/1984 Brendel 4,535,060 A 8, 1985 Comai (73) Assignee: Monsanto Technology LLC, St. Louis, 4,624,937 A 11/1986 Chou MO (US) 5,043,475 A * 8/1991 Fields, Jr. ........................ 562/17 5,087,740 A 2, 1992 Smith (*) Notice: Subject to any disclaimer, the term of this 5,094,945 A 3, 1992 Comai patent is extended or adjusted under 35 5,317,003 A 5/1994 Kassebaum et al. U.S.C. 154(b) by 0 days. (Continued) (21) Appl. No.: 13/370,625 FOREIGN PATENT DOCUMENTS Filed: Feb. 10, 2012 CN 1557820. A 12, 2004 (22) EP O274369 A1 T 1988 (65) Prior Publication Data (Continued) US 2012/O149569 A1 Jun. 14, 2012 OTHER PUBLICATIONS Database Caplus Chemical Abstracts Service, Columbus, Ohio, US; Related U.S. Application Data Database Accession No. 2005:626229, Abstract of Ma et al., CN 1557820, (2004).* (62) Division of application No. 1 1/368,344, filed on Mar. 3, 2006, now Pat. No. 8,129,564. (Continued) (60) Provisional application No. 60/659,001, filed on Mar. Primary Examiner — Karl J. Puttlitz 4, 2005, provisional application No. 60/713,948, filed (74) Attorney, Agent, or Firm — Senniger Powers LLP: on Sep. 1, 2005. Joseph A. Schaper (51) Int. C. (57) ABSTRACT AOIN 25/32 (2006.01) This invention relates generally to improved methods and AOIN 57/20 (2006.01) herbicidal glyphosate compositions for use in controlling the (52) U.S. C. growth of weeds and unwanted vegetation, and particularly CPC ................ A0IN57/20 (2013.01); A0IN 25/32 for use in controlling weeds in a crop of transgenic glypho (2013.01) sate-tolerant cotton plants by over-the-top, foliar application (58) Field of Classification Search of a herbicidal glyphosate formulation. None See application file for complete search history. 35 Claims, 11 Drawing Sheets £888 &ressis xii; R8sixts extier8X-rise fastsixties:x: xie Six ed irot 38 & 3rgii: 883& axes & kissistics: 3 sixis. : 8 US 9,364,003 B2 Page 2 (56) References Cited 2007/0O37708 A1 2/2007 Prosch et al. 2009, OO11515 A1 1/2009 Soleta et al. U.S. PATENT DOCUMENTS 2010/0273.656 A1* 10/2010 Sedun et al. .................. 504,319 5,463,175 10, 1995 Barry et al. FOREIGN PATENT DOCUMENTS 5,464,807 11, 1995 Claude et al. 5,543,383 8, 1996 Parker et al. JP O9227583 9, 1997 5,633,435 5, 1997 Barry et al. WO 9533379 A2 12/1995 5,668,085 9, 1997 Forbes et al. WO OO15037 A1 3, 2000 5,750.468 5, 1998 Wright et al. WO O236782 A2 5, 2002 6,040,497 3, 2000 Spencer et al. WO 03092360 A2 11/2003 6,083,878 T/2000 Brant et al. WO 2004O72235 A2 8, 2004 6,323,153 11, 2001 Smiley WO 2005O12515 A2 2, 2005 6,365,772 4, 2002 Cullen et al. WO 2005O16519 A1 2, 2005 6,417, 133 T/2002 Ebner et al. WO 2006096617 A2 9, 2006 6,448,476 9, 2002 Barry OTHER PUBLICATIONS 6,586,621 T/2003 Leiber et al. 6,603,039 8, 2003 Ebner et al. Repasi et al., Pesticide Science (1993), 39(4), 287-92.* 6,696,602 2, 2004 Aust et al. EPA Summary for Glyphosate intermediate (GI) 6,730,813 5, 2004 Hitzler et al. N-(Phosphonomethyl)iminodiacetic acid (PIA, MON 5735, CP 6,740,488 5, 2004 Rangwala et al. 41820) found at <http://www.epa.gov/chemrtk/pubs/summaries/ 6,956,005 10, 2005 Leiber glypintr/c14947rs.pdf>. Dec. 29, 2003.* 6,963,009 11/2005 Leiber et al. Tsui et al., Environmental Pollution 138 (2005) 59-68.* 7,049,270 5, 2006 Lennon et al. ................ 504,206 Adu-Tutu, K.O., et al., “Reduced Tillage and Crop Residue Effects on 7,932,419 4, 2011 Liu et al. ..... ... 562/11 Cotton Weed Control, Growth and Yield.” Arizona Cotton Report 2001/0051591 12, 2001 Ferrett et al. .................. 504,103 (P-138) May 2004, pp. 237-261. 2002fOO68836 6, 2002 Haupfear et al. Ambrus, et al., “Significance of Impurities in the Safety Evaluation of 2003. O104943 6, 2003 Lennon et al. Crop Protection Products.” Pure Appl. Chem., 2003, 75(7):937-973. 2003. O181332 9, 2003 Sedun et al. ............... 504,116.1 Kwiatkowski, M., “A Modern Weedkiller, 1991, Polish Technical 2004/0010160 1, 2004 Coleman et al. Review, 1992: 168294 CAPLUS, Abstract Only. 2004/OO97372 5, 2004 Abraham et al. ............. 504,127 McCloskey, W.B., et al., “Roundup Ready Flex Cotton: Glyphosate 2004/O1428.23 T/2004 Elsik et al. .................... 504,365 Tolerance and Weed Management 2002-2003.” Arizona Cotton 2004/O186022 9, 2004 Coleman ....................... 504,320 Report (P-138) May 2004, pp. 227-236. 2005/0059840 3, 2005 Haupfear et al. Herbicide Handbook, William K. Vencilled. Weed Science Society 2005/0223425 10, 2005 Clinton et al. of America, 8th Ed., 2002, pp. 69 and 329-330. 2005/0261130 11/2005 Lennon et al. Jones et al., “Tolerance of Transgenic Cotton to Tropical Applications 2006, OO2O143 1, 2006 Leiber of Glyophosate.” 1999, J. Cotton Sci, 3:19-26. 2006/0106248 5, 2006 Scaia et al. 2006/02294.66 10, 2006 Arhancet et al. * cited by examiner U.S. Patent Jun. 14, 2016 Sheet 1 of 11 US 9,364,003 B2 ?, ztt: gcr: aband, * *«??? U.S. Patent Jun. 14, 2016 Sheet 2 of 11 US 9,364,003 B2 88ssssssssssssssssss a----------------------------- 6er U.S. Patent Jun. 14, 2016 Sheet 3 of 11 US 9,364,003 B2 -------------&- *: s U.S. Patent Jun. 14, 2016 Sheet 4 of 11 US 9,364,003 B2 - CENTRIFUGE 2f 37 U.S. Patent Jun. 14, 2016 Sheet 5 of 11 US 9,364,003 B2 o K : &s (xixe is , U.S. Patent Jun. 14, 2016 Sheet 6 of 11 US 9,364,003 B2 ?uwazºspe?ua‘uo?anquasipº‘eae) U.S. Patent Jun. 14, 2016 Sheet 7 of 11 US 9,364,003 B2 & . s . S & S X------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ U.S. Patent Jun. 14, 2016 Sheet 8 of 11 US 9,364,003 B2 ----------------------------------------------------------&------------------ ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------8 U.S. Patent Jun. 14, 2016 Sheet 9 of 11 US 9,364,003 B2 U.S. Patent Jun. 14, 2016 Sheet 10 of 11 US 9,364,003 B2 & valid ses. C*x. -te8: U.S. Patent Jun. 14, 2016 Sheet 11 of 11 US 9,364,003 B2 : *:::::: :8: US 9,364,003 B2 1. 2 MTIGATING NECROSS IN TRANSGENIC lar agronomic and environmental importance is herbicide GLYPHOSATE-TOLERANT COTTON tolerance, in particular, tolerance to glyphosate herbicide. PLANTS TREATED WITH HERBICIDAL Glyphosate-resistant or tolerant crop plants may reduce the GLYPHOSATE FORMULATIONS need for tillage to control weeds, thereby effectively reducing soil erosion. Further, glyphosate-tolerant crop plants provide REFERENCE TO RELATED APPLICATIONS greater simplicity and flexibility in attaining adequate weed control. This application is a division of U.S. nonprovisional patent Glyphosate-tolerant cotton can be produced, for example, application Ser. No. 1 1/368,344, filed Mar. 3, 2006, and by introducing into the genome of the plant, the capacity to claims the benefit of U.S. provisional application Ser. No. 10 express various native and variant plant or bacterial EPSPS 60/659,001, filed Mar. 4, 2005 and U.S. provisional applica enzymes that have a lower affinity for glyphosate and there tion Ser. No. 60/713,948, filed Sep. 1, 2005, the entire con fore retain their catalytic activity in the presence of glypho tents of which are incorporated herein by reference. sate (See, for example, U.S. Pat. Nos. 5,633,435, 5,094,945, 4,535,060, 6,040.497 and 6,740,488). Glyphosate-tolerance BACKGROUND OF THE INVENTION 15 has been introduced into cotton plants and is a Successful product now widely used in cotton production. The current This invention relates generally to improved methods for commercial ROUNDUP READY cotton event designated controlling weeds in a crop of transgenic glyphosate-tolerant 1445 available from Monsanto Company provides excellent cotton plants by over-the-top, foliar application of a herbi resistance to glyphosate. Glyphosate is typically applied cidal glyphosate formulation. The present invention is further over-the-top (OTT) of ROUNDUP READY cotton from directed to herbicidal glyphosate compositions useful in prac emergence through the four leaf node stage of development ticing the weed control methods disclosed herein. (e.g., at rates of up to about 0.75 pounds glyphosate acid Cotton (i.e., Gossypium hirsutum) provides an ideal fiber equivalent per acre (Tb a.e./A or about 0.84 kg a.e./ha). for textile manufacture as well as oil for human consumption, ROUNDUP READY cotton varieties used in combination feed for livestock and base chemicals for a variety of indus 25 with ROUNDUP glyphosate herbicidal formulations have trial products. Cotton production is well-established in the become the standard program for weed management in cotton United States and many other areas of the world.
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