Recombinant Human MYLPF Protein Catalog Number: ATGP1097

Recombinant human MYLPF protein Catalog Number: ATGP1097 PRODUCT INPORMATION Expression system E.coli Domain 1-169aa UniProt No. Q96A32 NCBI Accession No. NP_037424 Alternative Names Myosin regulatory light chain 2 skeletal muscle isoform, Myosin regulatory light chain 2, skeletal muscle isoform, MRLC2, MYL11, Fast skeletal myosin light chain 2, MLC2B PRODUCT SPECIFICATION Molecular Weight 21.2 kDa (189aa) confirmed by MALDI-TOF Concentration 0.25mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl Purity > 85% by SDS-PAGE Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development. Recombinant human MYLPF protein, fused to His-tag at N-terminus, was expressed in E. 1 Recombinant human MYLPF protein Catalog Number: ATGP1097 coli and purified by using conventional chromatography techniques. Amino acid Sequence MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI THGDAKDQE General References Wang Y, et al. (2007) FASEB J. 21(9):2205-14. Gittings W, et al. (2011) J Muscle Res Cell Motil. 31(5-6):337-48. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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