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CV-Wstsai-16.Pdf CURRICULUM VITAE Wen-Hsi Tsai, Ph.D. (蔡文錫) Associate Specialist Virology Unit, AVRDC- The World Vegetable Center PO Box 42, Shanhua District, Tainan City, 74199 Taiwan, ROC 60 Yi-Min Liao, Shanhua District, Tainan City, 74151 Taiwan, ROC Phone: 886-(0)6-5837801 ext. 351; (O) 886-(0)6-3554662 Fax: 886-6-5830009 E-mail: wen-shi.tsai@ worldveg.org;[email protected] EDUCATION AND ACADEMIC DEGREES 2005-2008 National Chung Hsing University, Taichung, Taiwan Department of Plant Pathology 1991-1993 National Chung Hsing University, Taichung, Taiwan Graduate Institute of Molecular Biology 1987-1991 National Chung Hsing University, Taichung, Taiwan Department of Botany BSc, major in plant science, minor in molecular biology EMPLOYMENT 2009-present Associate Specialist, Virology Unit, AVRDC- The World Vegetable Center 1996-2008 Principal Research Assistant, Virology Unit, AVRDC- The World Vegetable Center LANGUAGES English, Chinese and Taiwanese EXPERIENCE WSTsai- 1 - 1996 - ongoing Begomovirus survey, detection, cloning and sequencing, including tomato, pepper, cucurbits, legume, eggplant, weeds, etc. Locations: Cambodia, Ethiopia, Indonesia, Honduras, Mali, Taiwan, Tanzania, Thailand and Vietnam ACIAR projects, Australia; COA projects, ROC; GTZ projects, Germany (principal/co-principal investigator) 2012 – ongoing Multilocation test for screening of tomato begomovirus resistances 2008 Targeting Taiwan, Thailand and Vietnam COA project, ROC (principal/co-principle investigator) 2012 – ongoing Multilocation trial for screening of pepper begomovirus resistances 2006 – 2009 Targeting Africa (Mali), central America (Honduras) and Thailand USAID/IPM CRISP project (principle/co-principle investigator) 2006-ongoing Screening and study of cucurbit virus resistances Targeting the Philippines, Taiwan and Thailand APSA, NSC, COA (principle/co-principle investigator) 2006-2010 Integrated disease management (IDM) for anthracnose, Phytophthora blight, and whitefly-transmitted geminivirus in chili pepper in Indonesia. ACIAR project (HORT/2004/048) (co-principle investigator) 2009-2010 Multilocation test for screening of cucurbit begomovirus resistances Locations: Taiwan and Thailand COA project, ROC (principle investigator) 2004 - 2008 Development of transgenic tomato with virus-derived resistance against tomato-infecting geminiviruses, NSC project, ROC (principal investigator) 2002 - 2009 ChiVMV genomic RNA detection, cloning and sequencing GTZ Chilli projects, Germany (co-principal investigator) 2005 - 2006 Development of transgenic tomato with virus-derived resistance against tomato-infecting CMV ABSPII project, USAID, USA (co-principal investigator) 2005 ToCV and TICV survey, detection, and resistance screening of the project intile, “Development and application of detection methods for transgenic plants and surveillance of newly emerging pathogens” BAPHIQ project, Council of Agriculture, ROC (principal investigator) 2004 - 2005 Development of transgenic tomato with CP and truncated CP gene against tomato leaf curl virus ABSPII project, USAID, USA (co-principal investigator) 2003 Dec Tomato virus survey, 1 week survey conducted in Vietnam and Cambodia 2000 Jun - Dec “Development of diagnostic probes for the identification of different strains of chilli veinal mottle virus (ChiVMV) in Taiwan”. Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ) WSTsai- 2 - project, Council of Agriculture, ROC (principal investigator) TRAINING 2011 July Training course in Project Management, India Office of the International Rice Research Institute (IRRI), New Delhi, India, 11-15 July 2011. 2003 Aug “New knowledge of biotechnology and chip technology”. hands-on training course (5 days), National Chung Hsing University 2002 Sep/Oct Construction of infectious geminivirus clones. Visiting Research Scholar (5 weeks) with Dr. Ali Rezaian, CSIRO Plant Industry, Adelaide, Australia 2000 Mar Hands-on workshop on virus elimination and indexing of garlic and shallot. (3 wk) 1990 Graduate Institute of Basic Medical Science, Chang Gung University, Taiwan 3 months scholarship of the National Science Council, Taiwan, ROC Study topic: “Erwinia pigment gene cloning and sequencing” WSTsai- 3 - PROFESSIONAL EXPERIENCE - survey of plant virus diseases in Cambodia, Ethiopia, Honduras, Indonesia, Mali, Taiwan, Tanzania and Vietnam - multilocation screening of begomovirus resistances (cucurbit, pepper and tomato) - plant virus diagnostics, epidemiology and host rang study - characterization of DNA viruses by PCR and nucleic acid hybridization - design of probes and primers for virus general and specific detection (including RNA and DNA viruses) - characterization of viruses by RT-PCR, IC-RT-PCR, Northern hybridization, ELISA and Western blot - cloning, sequencing and sequence analysis of geminiviruses, cucumber mosaic virus and potyviruses - development of infectious clones (geminiviruses) - resistance screening and rating of germplasm, breeding lines (potyviruses, geminiviruses, tospoviruses, cucumoviruses, tobamoviruses) - development of transgenic tomato resistant to CMV, potyviruses and geminiviruses using CP, antisense gene and gene silencing strategy - chloroplast isolation, study the interaction between plant virus and chloroplast - project proposal development and project management - supervising /training of research scholars in virological methods and research topics WSTsai- 4 - PUBLICATIONS Full-length paper 1. Knierim, D., Tsai, W. S., Deng,T. C., Green, S. K. and Kenyon, L.* 2013. Full-length genome sequences of four polerovirus isolates infecting cucurbits in Taiwan determined from total RNA extracted from field samples. Plant Pathology, 62:633-641. (doi: 10.1111/j.1365-3059.2012.02653.x) (NSC 97-2811-B-125-001 and NSC 98-2811-B-125-001) 2. Kadirvel, P., de la Pen˜a, R., Schafleitner, R., Huang, S., Geethanjali, S., Kenyon, L., Tsai, W. and Hanson, P.* 2013. Mapping of QTLs in tomato line FLA456 associated with resistance to a virus causing tomato yellow leaf curl disease. Euphytica, 190(2): 297-308. (doi 10.1007/s10681-012-0848-0) 3. Shih, S. L., Tsai, W. S., Lee, L. M. and Kenyon, L.* 2013. Molecular characterization of begomoviruses infecting Sauropus androgynus in Thailand. Journal of Phytopathology 161:76-85. (doi: 10.1111/jph.12028) 4. Sengoda, V. G.*, Tsai, W. S., de la Peña, R. C., Green, S. K., Liu, C. A., Kenyon, L. and Hughes, J. 2012. Expression of full length coat protein gene of Tomato leaf curl Taiwan virus is not necessary for recovery phenotype in transgenic tomato. Journal of Phytopathology 160:213-219. 5. Lin, C.-Y., Tsai, W. S., Ku, H.-M., and Jan, F.-J.* 2012. Evaluation of DNA fragments covering the entire genome of a monopartite begomovirus for induction of viral resistance in transgenic plants via gene silencing. Transgenic Research 21:231-241 (doi:10.1007/s11248-011-9523-9) (NSC95-2752-B-005-002-PAE) 6. Tsai, W. S., Shih, S. L., Kenyon, L., Green, S. K. and Jan, F.-J.* 2011. Temporal distribution and pathogenicity of the predominant tomato-infecting begomoviruses in Taiwan. Plant Pathology 60:787-799. (NSC94-2317-B-125-001, COA92AS-1.8.1-BQ-B4 and COA97AS-4.1.2-IC-I1) 7. Tsai, W. S., Shih, S. L., Venkatesan, S. G., Aquino, M. U., Green, S. K., Kenyon, L. and Jan, F.-J.* 2011. Distribution and genetic diversity of begomoviruses infecting tomato and pepper plants in the Philippines. Annals of Applied Biology 158:275-287. (NSC95-2317-B-125-001 and GTZ No.2001.7860.8-001.00) 8. Lin, C.-Y., Ku, H.-M., Tsai, W. S., Green, S. K. and Jan, F.-J.* 2011. Resistance to a DNA and a RNA virus in transgenic plants by using a single chimeric transgene construct. Transgenic Research 20:261-270. (NSC97-2317-B-005-004) 9. Chang, H.-H., Ku, H.-M., Tsai, W. S., Chien, R.-C. and Jan, F.-J.* 2010. Identification and characterization of a mechanical transmissible begomovirus causing leaf curl on oriental melon. European Journal of Plant Pathology 127:219-228. (COA98AS-9.1.2-BQ-B1) 10. Knierim, D., Deng, T. C., Tsai, W. S., Green, S. K. and Kenyon, L.* 2010. Molecular identification of three distinct Polerovirus species and a recombinant Cucurbit aphid-borne yellows virus strain infecting cucurbit crops in Taiwan. Plant Pathology 59(5):991-1002. (NSC 97-2811-B-125-001 and NSC WSTsai- 5 - 98-2811-B-125-001) 11. Tsai, W. S., Huang, Y. C., Zang, D. Y., Reddy, K., Hidayat, S. H., Srithongchai, W., Green, S. K. and Jan, F.-J.* 2008. Molecular characterization of the CP gene and 3’UTR of Chilli veinal mottle virus from South and Southeast Asia. Plant Pathology 57:408-416. (GTZ 2001.7860.8-001.00 and COA89-ST-6.2-BQ-90) Brief Report, disease note, new disease report and annotated sequence report 1. Tsai, W. S., Shih, S. L., Lee, L. M., Wang, J. T., Duangsong, U., and Kenyon, L.* 2013. First report of Bhendi yellow vein mosaic virus associated with yellow vein mosaic of okra (Abelmoschus esculentus) in Thailand. Plant Disease, 97(2):291. (COA101AS-4.1.1-ST.a1 and GIZ 11.7860.7-001.00) 2. Tsai, W. S., Hu, C.-J., Shung, D.-P., Lee, L. M., Wang, J. T. and Kenyon, L.* 2011. First report of Squash leaf curl Philippines virus infecting chayote (Sechium edule) in Taiwan. Plant Disease 95(9):1197. (COA99AS-4.1.1-IC-I1) 3. Tsai, W. S., Abdourhamane, I. K. and Kenyon, L.* 2010. First report of Pepper veinal mottle virus associated with mosaic and mottle diseases of tomato and pepper in Mali. Plant Disease 94(3):378. 4. Tsai, W. S., Abdourhamane, I. K., Knierim, D., Wang, J.T. and Kenyon, L.* 2010. First report of Zucchini yellow mosaic virus associated with leaf crinkle and yellow mosaic diseases of cucurbit plants in Mali. Plant Disease 94 (7):923. 5. Shih, S. L., Tsai, W. S., Lee, L. M., Wang, J. T., Green, S. K. and Kenyon, L.* 2010. First report of Tomato yellow leaf curl Thailand virus associated with pepper leaf curl disease in Taiwan. Plant Disease 94(5):637. (COA97AS-4.1.2-IC-I1) 6. Tsai, W. S., Shih, S. L., Green, S. K., Lee, L. M., Luther, G. C., Ratulangi, M., Sembel, D. T. and Jan, F.-J.* 2009. Identification of a new begomovirus associated with yellow leaf curl diseases of tomato and pepper in Sulawesi, Indonesia.
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