Sample Preparation Product Guide Your Data Depend on Your Preparation Tools

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Sample Preparation Product Guide Your data depend on your preparation tools. Get today’s samples ready for tomorrow’s analyses.

As our understanding of nature’s complexity and heterogeneity has deepened, there has evolved a demand for more sensitive, multidimensional sample analysis. Measuring a single sample with a single parameter, at a single time point, is insufficient to characterize a system. To enable measurement of multiple, dynamic parameters of multiple samples, Merck Millipore has developed sample preparation solutions that improve signal-to-noise ratios, increase throughput and/or decrease hands-on time.

Refer to this guide to sample preparation products that help you get your samples ready for sensitive analyses, such as UHPLC, next generation sequencing, flow cytometry, immunoassays, mass spectrometry and gravimetric analysis.

Selection guides to help Featured products and applications: you find the right tools for Amicon® Pro Protein Purification System 82 your experiments: Amnis® Imaging Flow Cytometers 48 Antibodies 124 Agarose Selection Guide 84 Buffer Optimization 94 Amicon® Ultra Selection Guide 97 CellASIC® ONIX Platform for 3D Cell Culture 45 Extraction Selection Guide 68 Direct Detect® Spectrometer for Lipid Analysis 112 Filter Holder Selection Guide 126 Elispot with MultiScreen® Filter Plates 37 Membrane Selection Guide 170 HTS High Performance Liquid Chromatography 148 Multiwell Plate Selection Guide 218 Immobilon® Membrane for Fluorescent Detection 117 Non-Sterile Millex® Selection Guide 150 Improved Chromatin Immunoprecipitation 66 Sterile Millex® Selection Guide 18 Lab Water Purification 215 Ultrafiltration Device Selection Guide 96 Microcon® Filters for DNA Sample Preparation 51 Ultrafiltration Membrane Selection Guide 96 Multiplexed Assays for Cancer Research 123 Muse® Cell Analyzer and guava easyCyte™ Flow Cytometers 47 One-step Lysate Preparation Speeds Protein Purification 70 Radiation Monitoring 136 SmartFlare™ Probes for Live Cell RNA Detection 64 SNAP i.d.® 2.0 Antibody Recovery 120 Stericup® Filtration Device 12 Sterile Millex® 33 mm Syringe Filters 17

Vacuum Manifold for MultiScreen®HTS Filter Plates 39 Table of Contents

Overview of Membrane Technology page 4

Cellular Sample Preparation page 9 Prepare: Trusted sterile filtration devices 9 Grow: Cell culture plates, inserts, vessels 23 Analyze: Unique cell assay tools and analysis platforms 33

Genomic Sample Preparation page 49 Prepare/Clone: PCR, DNA preparation tools, cloning kits 49 Transfect/Express: Reagents, competent cells, gene expression kits 57 Detect: Agarose, buffers, reagents 61

Protein Sample Preparation page 67 Extract: Extraction reagents, kits, inhibitors, detergents 67 Purify: Purification kits and pipette tips, agarose beads, magnetic beads 79 Optimize/Concentrate: Buffer optimization tools, centrifugal filters, ultrafiltration discs 93 Quantify/Detect: Spectrometer, membranes, reagents, Western blotting system 111

Analytical Sample Preparation page 125 Collect: Filter holders, monitors, solvent dispensers 125 Prepare: Syringe filters, membrane filter discs, solid phase extraction, pumps and accessories 147 Test: Chromatography columns, sampling kits, lab water 207

Appendix page 217 Multiwell Plate Selection Guide 218 Tables of Chemical Compatibility 220 Index by Catalogue Number 235 Index by Product/Application 246 Overview of Membrane Technology

Choosing the proper membrane device for your laboratory process is an important first step in any successful analysis. To help you select the filtration device best suited to your specific application, we have included this overview of microfiltration (MF) and ultrafiltration (UF) membrane technologies. You will find descriptions of the principal theory behind each technology, definitions of associated terms and explanations of the key criteria you should consider when choosing filtration devices.

For more information, ask our technical service team. Visit www.merckmillipore.com/techservice for contact information or browse our extensive online technical library at www.merckmillipore.com.

4 www.merckmillipore.com Membrane Processes

The advantages of using membranes for sample preparation are numerous. Separation occurs via selective diffusion of a component through membrane pores, with either pressure, vacuum or concentration gradient being the driving force for this diffusion. As a result, membrane-based separation is relatively energy-efficient. Also, precision both in membrane manufacturing, as well as in application of pressure or vacuum, allow great control over separation parameters, such as particle size, volume-to-surface area ratio, and flux. Finally, the relatively gentle conditions required for membrane separations allow for separation of chemically reactive or unstable species—for example, biomolecules.

Reverse Osmosis Ultrafiltration Microfiltration Clarification Figure 1. Membrane pore size affects type 0.0001 µm 0.001 µm 0.01 µm 0.1 µm 1 µm 10 µm 100 µm of particles retained.

0.2 kDa 200 kDa 20,000 kDa

Sugars Proteins B. diminuta Red Smallest Visible Blood Cell Particle

Amino Acids Nucleotides Carbon Black Yeast Pollens

Oligo- Salts Polio Mycoplasma E. coli Clouds, Fog nucleotides Virus

Antibiotics Bacteria Human Mammalian Cells Hair

Microfiltration Ultrafiltration UF is far gentler to solutes than processes Microfiltration (MF) is the process of Ultrafiltration (UF) is the process of separating such as precipitation, and it is more efficient removing particles or biological entities in extremely small particles and dissolved because it can simultaneously concentrate the 0.025 μm to 10.0 μm range from fluids by molecules from fluids. The primary basis for and desalt solutes. It does not require a phase passage through a microporous medium such separation is molecular size, although in change, which often denatures labile species, as a membrane filter. Although micron-sized all filtration applications, the permeability such as proteins, DNA and RNA, and UF can particles can be removed by use of non- of a filter medium can be affected by the be performed either at room temperature or membrane or depth materials such as those chemical, molecular or electrostatic properties in a cold room. UF is thus an ideal method, found in fibrous media, only a membrane of the sample. UF can only separate molecules not only for preparing protein samples, filter having a precisely defined pore size can which differ by at least an order of magnitude but also for manipulating nucleic acids, ensure quantitative retention (Figure 2). in size. Molecules of similar size cannot be including molecular cloning and purifying Membrane filters can be used for final separated by UF (see Figure 1). Materials plasmids. DNA and RNA samples with starting filtration or prefiltration, whereas a ranging from 1 to 1,000 kDa molecular weight concentrations as low as 5 ng/mL can be depth filter is generally used in clarifying (MW) are retained by certain UF membranes, routinely concentrated in minutes with 99% applications where quantitative retention is while salts and water will pass through. recovery of starting material. Solvents can be not required or as a prefilter to prolong the Colloidal and particulate matter can also be exchanged by diafiltration. In this process, the life of a downstream membrane. retained. UF membranes can be used both sample is concentrated, then diluted to the to purify material passing through the filter original volume with the desired buffer and and also to collect material retained by the concentrated again, thus “washing out” the filter. Materials significantly smaller than the original solvent. pore size rating pass through the filter and can be depyrogenated, clarified and separated from high molecular weight contaminants. Materials larger than the pore size rating are Figure 2. Depth filters (Left) have a random retained by the filter and can be concentrated network of flow channels that trap particles or separated from low molecular weight throughout the depth of the matrix. Membrane filters (Right) have a geometrically contaminants. regular matrix of pores. Particles are retained by size exclusion. www.merckmillipore.com 5 Membrane Attributes

Microfiltration (MF) Pore Size Throughput (Filtration Capacity) When choosing the optimal microfiltration 0.22 µm Membrane 0.1 µm Membrane DMEM, 10% FBS, 47 mm Disc DMEM, 2.5% NBCS, 47 mm Disc membrane for your application, pore size is 3000 500 one important variable. A MF membrane’s Express Express VVPP pore size rating, typically given in units of 2500 CA 400 PES G PES G microns, indicates that particles larger than 2000 GS GV the rating will be retained. MF membrane 300 1500 pores range from 0.025 μm (used for 200 microdialysis of nucleic acids and proteins), (mL) Volume 1000 (mL) Volume to 0.1–0.22 μm (used for sterile filtration), 500 100 to 0.3–0.45 μm (used for clarification or 0 0 microbiological retention), to 0.45–8.0 μm 0 5 10 15 20 0 400 800 1200 (used for air and fluid particle monitoring). Time (Minutes) Time (Seconds) See page 171 for a complete pore size selection chart. Figure 3. Several membrane types were compared for throughput or filtration capacity. 47 mm discs were challenged with either DMEM, 10% FBS (0.22 μm filters, left), or with DMEM, 2.5% NBCS (0.1 μm filters, right). In both cases, the Millipore Express® (PES) filters exhibited the highest filtration Membrane Diameter capacity. The filter diameter required for a particular application depends on the volume of the solution being filtered and the flow rate (flux). Flux values are listed for each product in this Prefilters Chemical Compatibility application guide to allow you to choose the Another way to extend the lifespan and and Extractables appropriate membrane diameter. throughput of your filter is to add an Researchers use MF membranes to remove upstream prefilter. Organic solvents are most dissolved gases and particulates from a Membrane Throughput or often prefiltered through polypropylene variety of aqueous solutions and organic Filtration Capacity prefilters, available in 0.6 – 30 μm pore sizes. solvents. How rapidly and efficiently you can filter These polypropylene filters can also be used your solution also depends on the filtration for aqueous solutions if they are first wetted Most lab filtration applications also demand capacity (or throughput). When filtering large with alcohol. Aqueous solutions are most that filtered solutions do not retain impurities volumes or heavily contaminated solutions, widely clarified with glass fiber prefilters from the filter unit itself. Extractability is the such as crude cell lysates, membrane pores with binder resin, which have superior wet degree to which impurities can be leached can fill up to maximum capacity, slowing the strength and can collect dirt from heavily from the membrane into the solution; rate of filtration. Of the several membranes contaminated liquids. If you are concerned membranes and filter units made from low- available, Millipore Express® (PES) membrane about glass fibers shedding into the filtrate, extractable polymers with broad chemical filters have the highest filtration capacity use Type-RW prefilters, which are made from compatibility ensure that filtrates perform (Figure 3). These filters have an asymmetric non-shedding materials (polymers of cellulose well in downstream applications. pore structure – at the top, where filtration esters formed around a polyester web). Type- starts, the pores are larger. The pore size then RW prefilters have equivalent dirt-holding For your particular application, see the decreases from the top face of the filter to the capacity to their glass fiber counterparts. Filter Selection Guide on page 170 or the bottom, where the pore size is small enough comprehensive chemical compatibility chart to retain bacteria (0.1 μm or 0.22 μm). This starting on page 220. asymmetric pore design traps large particles at the top so that they do not clog the tight pores on the retentive side of the membrane, enhancing the filtration capacity and useful life of the filter.

6 www.merckmillipore.com Adsorption relatively narrow range of sizes. In addition, unincorporated single-stranded primers from Nonspecific adsorption of molecules to slender, linear molecules (e.g., nucleic acids) double-stranded DNA fragments is required, filter membranes hinders reproducibility may find their way through pores that will the molecular weights of the primer and DNA in many analytical processes. For example, retain a globular species of the same weight. fragment should differ by at least an order of adsorption of a dissolved compound to a Retention can also be affected by hydration, magnitude for efficient separation. membrane during sample filtration will result counterions, steric effects, macromolecular in underestimation of the dissolution rate of conformation changes, solute-solvent Concentration Polarization this compound. interactions and solute-solute interactions. Another factor affecting retention Nevertheless, NMWL has proven to be an characteristics is the potential for membrane MF membranes are also often used effective general indicator of membrane fouling, or concentration polarization. This to filter cell culture media containing performance for globular proteins. occurs when there is an accumulation of protein additives, such as those in serum the retained solute on the surface of the supplements. Researchers must be confident It is best to choose a device with cut-off at membrane. At high concentrations, a gel layer that when cell culture solutions are filtered, about one half of the molecular weight of the forms that can act as a secondary membrane either to ensure sterility or to remove protein to be concentrated. This maximizes (Figure 4). This may interfere with passage of particulates, the proteins are not adsorbed protein recovery and minimizes filtration time. the molecules through the membrane and to the membrane and lost. For example, can adversely affect the flow rate. During concentrations of growth factors needed Nucleotide Cut-Off (NCO) concentration polarization, the gel layer to maintain special cell types must remain By virtue of the rod-like three-dimensional on the membrane surface superimposes constant before and after filtration. Protein structures of nucleic acids or polysaccharides, its own rejection characteristics on those binding is also undesirable in filters used to these types of molecules require a tighter of the membrane. Usually, concentration clarify cell lysates before protein purification. membrane (with a smaller cut-off) than polarization increases retention of lower globular proteins of the same molecular molecular weight species. A membrane with For dilute protein solutions, keep membrane weight. It is therefore convenient to consider a 100 kDa NMWL may reject 10–20% of diameter to a minimum to further avoid the membrane retention characteristics of albumin in a 0.1% solution of pure albumin. adsorption. nucleic acids as being related to their length However, in the presence of larger solutes (in nucleotides) rather than their molecular such as IgG, it may reject 90% of the albumin. Ultrafiltration (UF) weight. Several additional factors affect the Concentration polarization makes it very Nominal Molecular Weight Limit recovery of nucleic acid fragments from a difficult to use UF for solute fractionation UF membranes are rated according to the membrane of a given NMWL. These factors unless the solutes to be separated differ in nominal molecular weight limit (NMWL), include the strandedness of the DNA or RNA size by at least an order of magnitude. also sometimes referred to as molecular molecule, whether the DNA is linear, relaxed weight cut-off (MWCO). The NMWL indicates or supercoiled (for plasmid), the ionic strength that most dissolved macromolecules with of the solvent, the velocity of the process molecular weights higher than the NMWL will stream over the membrane and the nature be retained. An ultrafiltration membrane with of the driving force. The overall effect is that a stated NMWL should retain (reject) at least optimal nucleic acid recovery is achieved 90% of a globular solute of that molecular in low-salt buffers run under conditions weight in daltons. A lower NMWL increases of relatively low velocity (e.g., low vacuum rejection but decreases the filtration rate for pressure or low g-force). the same membrane material. If the DNA sample is in the presence of high Retention and product recovery are a function salt (or the device is run at a higher-than- recommended g-force), a significantly Ultrafiltration separates proteins from soluble of a variety of other factors, including the salts. “Concentration Polarization” slows down reduced DNA recovery may be observed. molecular shape and size of the molecule, filtration. The proteins form a gel layer on the electrostatics, sample concentration and Under these conditions, higher DNA or RNA membrane surface. composition, operating conditions and device recovery can be achieved by using a tighter or system configuration. Two membranes membrane. However, it will take significantly Figure 4. Concentration polarization at high concentrations. may have the same NMWL but will exhibit longer to complete the purification. For different retention of molecules within a applications such as PCR where removal of 7 www.merckmillipore.com Flux (UF Flow Rate) controlled by polarization (e.g., when the Fouling A membrane’s flux is defined as the flow resistance of the gel layer is much larger Flux decrease due to concentration rate divided by the membrane area. Using than that of the membrane), flux will reach polarization should not be confused with membranes with higher NMWL ratings will a plateau and may actually decrease with the effect of membrane fouling. Fouling is increase the flow, but at the same time lower increases in pressure. usually the deposition and accumulation the retention. A membrane should be selected of submicron particles and solute on the for required rejection, consistent with desired Concentration membrane surface and/or crystallization and flow rate. This is determined by surface area, When concentration of the retained precipitation of smaller solutes on or within macrosolute type, solubility, concentration species is very low, flux is independent of the pores of the membrane. There may be a and diffusivity, membrane type, temperature concentration. As solute concentration rises chemical interaction with the membrane. effects on viscosity and, to some extent, during operation, increased viscosity and the pressure. When concentration polarization polarization effect cause flux to decrease. Importance of recovery is rate-controlling, flux is affected by solute While rejection is used to characterize concentration, fluid velocity, flow channel Temperature membrane performance, it does not always dimensions, and temperature. Increasing the operating temperature directly correlate with solute recovery from a normally increases UF rates. A higher sample or volume. Actual solute recovery—the Effects of Operating Parameters temperature increases solute diffusivity amount of material recovered after UF—is on Flux (typically 3–3.5% per degree Celsius for generally based on mass balance calculations. Pressure proteins) and decreases solution viscosity. When ultrafiltering dilute protein solutions Common practice is to operate at the highest Since adsorption is a direct function of or colloid suspensions, flux will increase temperature tolerated by the solutes and the membrane and device surface area, device with increasing transmembrane pressure equipment. size must be considered when recovery (TMP). These effects are most apparent when is samples should be concentrated with operating under controlled positive pressure, pH membranes of minimal surface area, as long such as when using a stirred cell. When the Changing solution and/or pH often changes as reasonable flow rates can be achieved. process is membrane-controlled (i.e., when molecular structure. This is especially true the resistance of the gel layer is much smaller for proteins. At its isoelectric point, a protein than that of the membrane), the flux-pressure begins to precipitate, causing a flux decrease. relationship is linear. When the process is

Mode of Operation

Normal vs. Tangential streams, clarifying prefiltration, and virus/ However, in this case, the retained protein separations. components do not build up at the surface of Flow Filtration the membrane. Instead, they are swept along In normal flow filtration (NFF), fluid is In tangential flow filtration (TFF), the fluid by the tangential flow. This feature of TFF convected directly toward the membrane is moved tangentially along the surface of makes it an ideal process for finer sized-based under an applied pressure or vacuum. the membrane. An applied pressure serves separations. Although TFF is more commonly Particulates that are too large to pass through to force a portion of the fluid through the associated with large-scale processing, the pores of the membrane accumulate at membrane to the filtrate side. As in NFF, centrifugal UF devices with vertical membrane the membrane surface or in the depth of the particulates and macromolecules that are too panels, such as Amicon® Ultra devices, also filtration medium, while smaller molecules large to pass through the membrane pores are benefit from a TFF-like mode of separation, pass through to the downstream side. NFF can retained on the upstream side. particularly in a swinging bucket rotor. be used for sterile filtration of clean

8 www.merckmillipore.com Prepare, grow and analyze with advanced systems for every cell Cellular culture challenge. To complement our trusted sterile filtration tools, Merck Millipore supports both conventional and emerging formats Sample for mammalian cell culture with innovative solutions ranging from inserts and filter plates to Preparation optimized platforms for dynamic microenvironment control.

Prepare Grow Analyze

With industry-leading, innovative designs for filtration devices and over 50 years of membrane technology expertise, Merck page 23 page 33 Millipore offers a wide range of solutions to make the repetitive yet critical steps of preparing cells and media accurate, quick and easy. Prepare Cell 10 v r It pr t The Efficient media sterilization and storage up to 1L Stericup® and Steritop® Filter Units fromCertified anISO®9001/cGMPenvironmentwater quality Water for CellCulture eceiver he olumes

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Simply better membrane technology, simply better Stericup® filters.

Don’t take risks with your media. If your Trust Stericup® filters with NEW improved sterile filtration membranes are clogging and/ Millipore Express® PLUS membrane. Our or binding serum proteins in your media, your most popular Stericup® membrane, Millipore cells risk: Express® PLUS polyethersulfone (PES), now • Cellular damage delivers even faster filtration rates while • Exposure to toxins reducing overall protein and reagent loss. • Suboptimal cell growth

Learn more at: www.merckmillipore.com/sterilefiltration

Membrane design makes a difference.

Pore Membrane Membrane Main Membrane Structure Top Bottom Features Millipore Express® • High flow rate PLUS PES • Low protein binding (polyethersulfone) • Low volume retention Asymmetric Cell

Protein Binding by Membrane Type Low Protein Binding: Minimizes Protein Loss 200 Durapore® (PVDF)

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Sterile 33 mm Millex® Filters 20% more filter surface than 25 mm filters for significantly higher flow rate and throughput

Millex® syringe filters provide convenient sterilization of small volumes and are ideal for solutions such as antibiotics and tissue culture additives. Their unsurpassed quality and consistency of results have led to the development of many sample preparation methods that specify Millex® filters. Now sterile Millex® filter units are available in larger 33 mm housing with either MCE, PVDF or PES membranes for increased filtration performance and ease of use.

33 mm Millex® Filter Advantages Cell Faster flow rate for greater throughput The larger filter surface area increases flow Prepare rate and throughput. It also makes it easier to filter solutions because it reduces the pressure Preserving these additives translates into Automated ISO certified cleanroom required to empty the syringe. consistently intact, sterile cell culture media, manufacture ensuring reproducible cell growth, behavior Millex® filters are manufactured in a closed, Exceptionally high fluid recovery and downstream results. controlled environment using an automated In addition to improving filtration process. Human hands, which can introduce throughput, Millex® 33 mm filters also have Higher pressure rating for speed and variability, never touch the filter during an exceptionally low hold-up volume (less reduced risk of bursts assembly. A Certificate of Quality included in than 100 µL) despite the large size of the Millex® filters have a very high maximum each box describes our quality standards membrane surface area and housing, so there housing pressure of 10.5 bar (150 psig), in detail. is minimal sample loss within the device which means you can filter solutions faster during filtration. than before and practically eliminate the risk of bursts due to overpressurizing the fluid Choice of membranes Millex® 33 mm filters are available Ultra-low protein binding maximizes flow, which can often happen with syringe with three membranes: recovery and accuracy filtration. Whether you choose to use the fastest flow, • Millipore Express® PLUS (PES) low-protein-binding Millipore Express® PLUS Color-coded for instant visual membrane (PES) membrane-containing Millex® 33 mm identification • Durapore® (PVDF) membrane filters or the ultra-low protein binding version The color-coded band on the Millex® housing • Mixed cellulose esters (MCE) containing our Durapore® PVDF membrane, clearly indicates which membrane is inside, so membrane you will be assured maximum recovery of you don’t have to read labels or risk confusing soluble proteins, such as serum components similar-sized devices on the bench. typically added to cell culture media.

For sterile Millex® filter ordering information, see page 20. www.merckmillipore.com 17 Sterile Millex® Syringe Filter Selection Guide

Research Applications Tissue culture media and additives / Buffers / DMSO / Biological solutions

Medical Applications Drugs / Vitamins / Clinical solutions

Process Housing and Diameter Volume Sterilization 50 100 250 1,000 units/ Membrane Pore Size (µm) (mm) (hold-up) Method CE (M) units/pk units/pk units/pk pk Durapore® 0.1 µm Sterile 33 mm 100 mL Modified Acrylic, ● SLVV033RS (PVDF) Filtration & (<100 µL) RS ● SLVM33RS Membrane Mycoplasma Lowest binding removal membrane for 0.22 µm Sterile 33 mm 100 mL Modified Acrylic, ● SLGV033RS SLGV033RB SLGV033RK protein-rich Filtration (<100 µL) RS ● SLGVM33RS solutions 13 mm 100 mL HDPE, EO ● SLGV013SL (<100 µL) 4 mm 100 mL HDPE, EO SLGV004SL (<100 µL) 0.45 µm 33 mm 100 mL Modified Acrylic, ● SLHV033RS SLHV033RB SLHV033RK Clarification of (<100 µL) RS ● SLHVM33RS sterile solutions 13 mm 100 mL HDPE, EO ● SLHV013SL (<100 µL) 4 mm 100 mL HDPE, EO SLHV004SL (<100 µL) 5.0 µm 25 mm 100 mL PVC, EO SLSV025LS Clarification of (<100 µL) Cell Sterile Solutions Millipore 0.22 µm Sterile 33 mm 200 mL Modified Acrylic, ● SLGP033RS SLGP033RB SLGP033RK Express® PLUS Filtration (<100 µL) RS ● SLGPM33RS (PES) Membrane 25 mm 100 mL PVC, EO ● SLMPL25SS* Fast flow and (<100 µL)

Prepare ● SLMP025SS low binding for cell 0.45 µm 33 mm 200 mL Modified Acrylic, ● SLHP033RS SLHP033RB culture media Clarification of (<100 µL) RS ● SLHPM33RS preparation sterile solutions MF-Millipore™ 0.22 µm Sterile 33 mm 100 mL Modified Acrylic, ● SLGS033SS SLGS033SB* (MCE) Filtration (<100 µL) RS ● SLGSM33SS Membrane 25 mm 100 mL PVC, EO SLGSV255F Most referenced (<100 µL) SLGL0250S* general purpose 0.45 µm 33 mm 100 mL Modified Acrylic, ● SLHA033SS SLHA033SB membrane Clarification of (<100 µL) RS ● SLHAM33SS sterile solutions 0.80 µm 33 mm 100 mL Modified Acrylic, ● SLAA033SS SLAA033SB Clarification of (<100 µL) RS ● SLAAM33SS sterile solutions PTFE, 0.2 µm Sterile 13 mm 10 mL HDPE, EO SLLG013SL Hydrophilic Filtration of (<25 µL) Broad chemical DMSO 25 mm 100 mL HDPE, EO SLLG025SS compatibility (<100 µL)

CE = CE Marked, (M) = Medical device, * Male Luer-Lok® HDPE = High-density polyethylene, PVC = Polyvinyl chloride, RS = Radiosterilized, EO = Ethylene Oxide-treated

18 www.merckmillipore.com Sterile Millex® Syringe Filter Selection Guide

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Pore Housing and Size Diameter Sterilization Inlet Outlet Membrane (µm) (mm) Method Connection Connection 10 units/pk 25 units/pk 50 units/pk 100 units/pk Durapore® 0.22 µm 25 mm PVC, EO FLL MLS SLGVS25PS (PVDF) SE2M407H0** Membrane SE2M407H0** Super MLL SLGVS25US hydrophobic SLGVS25XS membrane for transducer SLGVS25XS protection SPIKE SLGVS25LS Fluoropore™ 0.20 µm 25 mm PVC, EO FLL MLS SLFG025LS (PTFE) MLL SLFG25BS Membrane Needle SLFGN25VS Hydrophobic chemistry for PP, MLS SLFG02550 gas filtration Autoclavable 0.20 µm 50 mm PP, SHB SHB SLFG05010 Autoclavable 1/8 in. NPTM SLFG55010 SHB (latex) 1/8 in. NPTM SLFG65010 SLFG65000 1/8 in. NPTM 1/8 in. NPTM SLFG75010 SLFG75000 SHB (silicone) SHB (silicone) SLFG85010 SLFG85000 0.45 µm 50 mm PP, SHB SHB SLFH05010 SLFH05000 Autoclavable Cell 1.0 µm 50 mm PP, SHB SHB SLFA05010 SLFA05000 Autoclavable Prepare **Solvent resistant housing PVC = Polyvinyl Chloride, PP = Polypropylene, EO = Ethylene Oxide FLL = Female Luer-Lok® outlet, FLS = Female Luer-slip™, MLL = Male Luer-Lok® outlet, MLS = Male Luer-slip™, SHB = Stepped Hose Barb

www.merckmillipore.com 19 Prepare Cell 20 Faster flow rates andhigher recoveries Sterile Syringe Millex® Filtersfor Liquid and AirSterilization Product Performance • Tailored F mar a quality aut Mille minimize with arange ofsamples andsolvents to pressures andare chemically compatible but they alsowithstand higheroperating minimal hold-upv and filt sample sol syr wit Available

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Prepare Grow Analyze

Not only do our cell culture devices and surfaces provide the most convenient, reliable, analysis-ready page 9 cell cultures, but they also yield cell page 33 growth, structure, and function that more closely mimic what occurs in vivo. Spend less time growing cells and fumbling with clumsy devices and more time on your research. Grow Cell 24 moreFor information visit: www.merckmillipore.com/cellculture Membrane-based culture for optimum growth anddifferentiation Millicell® 24&96-Well Plates CellCulture Insert Millicell®-24-Well Plates andAccessories pol Millicell®-96 Millicell®-96-Well Plates andAccessories pol Millicell®-24 Description Ordering Information 96-Well pol Millicell®-96 pol Millicell®-24 Single-Well 24-Well ycarbonate ycarbonate yethylene terephthalate yethylene terephthalate

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assays assays PCF CM HA Millicell® Standing Inserts Millicell® Standing Millicell® Hanging Inserts PET Organotypic Ordering Information Ordering Description Optimized cell growth, attachment and differentiationattachment cell growth, Optimized Millicell® Cell Culture Inserts Culture Cell Millicell® www.merckmillipore.com For more information visit: www.merckmillipore.com/cellculture visit: For information more Product Performance Product cells can access media fr cells can access mimic what occurs in closely more and function . vivo cell monolayer. sides of the both study sible to With and basolateral sides. Cell growth, structure, structure, Cell growth, sides. and basolateral Features & Benefits • For Grow Cell 26 From culture to analysis allinoneEZSLIDE Millicell® EZSLIDES For moreFor information visit: www.merckmillipore.com/cellculture sim br Easily ber from the slideprior to fixingor staining. There’s andviewstain your samples allinonedevice. Simplify c hamber Growing Area perWell, cm Millicell® Millicell® Millicell® Millicell® Millicell® Description Ordering Information Sterilization Compatibility Materials Media Volume Range, mL Chamber Capacity Specifications eaking ply

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For multidimensional analysis of cellular phenotypes with respect to time, space and changing page 9 page 23 conditions, we provide reagents, platforms and devices that successfully meld sophisticated technology with ease of use and optimization. Analyze Cell For High throughput assays for migration, invasion andmore 34 MultiScreen® Plates for Cell-BasedAssays MultiScreen® Single-well Culture Tray MultiScreen® Accessories MultiScreen®-MIC Description Ordering Information

information

96-well

Plate

Receiver

visit:

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Plate P Chemistry olycarbonate without area. sacrificing membrane surface increase throughput 4xover 24-well systems, membrane pore sizes. The96-well plates andarecompatibility available inthree filter plates are designed for broad assay transmigration assays. MultiScreen® -MIC cellgrowthsupport for co-culture and with suspension andadherent celllinesand in arange ofpore sizes to assays support MultiScreen® filter plates andkitsare available increase throughput andenableautomation. background through more thorough washing, decrease sample-to-sample variability, reduce science anddrug research discovery can Using filter plates for cell-basedassays inlife Filtration area, cm² Sterility Specifications Styrene S Styrene Device Material Configuration Pore Size (µm) Qty/Pk Catalogue No. Qty/Pk Pore Size (µm) Configuration Device Material tyrene 1 well 96 well 96 well and Assa Mig • • Range • Incubate, &Benefits Features Cell Applications • Proven Chemotaxis 0.28 Sterile sy f simult Compatible and chemotaxis assays ormats available for invasion, migration,

ration Viability Time-Resolved ys, stems 3.0 5.0 8.0

Whole

aneously, saving timeandmoney

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Cell Analyze 35 MAIPS4510 MSHAS4510 MSIPS4W10 MSIPN4510 MSIPN4W50 MSIPN4550 MSIPS4510 10 S2EM004M99 10 50 Qty/Pk Catalogue No. Clear 10 White ClearWhite 10 ClearWhite Clear 10 Clear 50 10 Color Acrylic Acrylic Styrene Acrylic Acrylic Acrylic Acrylic Acrylic Material distribution

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Sterile Sterile Sterile Non-Sterile Non-Sterile Non-Sterile Sterile Sterility morphology

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Uniform Compatible PVDF PVDF • • Easy • Applications Elispot Features& Benefits • Validated

Filter Plates and Antibody Pairs Antibody and Plates Filter

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MultiScreen® MultiScreen®-IP MultiScreen® Filter Plates Filter MultiScreen® MultiScreen® Ordering Information Ordering Description MultiScreen® he years, this assay has been optimized optimized has been assay this he years, emovable underdrain to allow for easy for allow to underdrain emovable membrane access. membrane r high protein-binding capacity low with high protein-binding sensitivity staining, reliable and background The plates reproducibility. high lot-to-lot enhanced imaging on a designed for are and including Zeiss of systems range AID www.merckmillipore.com Product Performance Product t of specifically introduction the through reader designed antibodies, automated membrane Elispot and 96-well systems, MultiScreen® plates. Improved spot recovery and spot definition recovery and spot spot Improved immunospot) (enzyme-linked The Elispot specific of detection powerful provides assay immune Elispot MultiScreen® Elispot Analyze Cell 36 For TNFα TNFα IFNγ IFNγ IL-12p40 GM-CSF IL-10 IL-6 IL-6 IL-5 IL-5 MultiScreen® IL-4 MultiScreen® SealingTape, clear IL Elipunch Kit S IL-2 Elispot Matched AntibodyPairs MultiScreen® Tool Accessories MultiScreen® MultiScreen® MultiScreen®-HA U MultiScreen®-IP Description Ordering Information -Continued trips -4 nderdrain

Elispot Elispot Elispot Elispot Elispot Elispot Elispot Elispot Elispot

Elispot Elispot Elispot

Elispot Elispot information

Antibody Antibody Antibody Antibody Antibody Antibody Antibody Antibody Antibody

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PVDF PVDF Sterility Non-Sterile Sterile Material Acrylic Styrene Acrylic Color la 10 Clear la 10 Clear White www.merckmillipore.com 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit 100 1 kit 1 1 kit 1 Qty/Pk Catalogue No. Catalogue Qty/Pk 10 10 ELI-018-M ELI-018-H ELI-016-M ELI-016-H ELI-014-M ELI-012-H ELI-010-H ELI-008-M ELI-008-H ELI-006-M ELI-006-H ELI-004-M MATAHCL00 ELI-004-H MELIPUNCH ELI-002-M MSPLRS096 M8IPS4510 M8IPFRAME MAHAS4510 MAIPSWU10 Application Spotlight

MultiScreen® plates for Elispot assays: State-of-the-art tools for functional immunology

A. CEF stimulated B. Plate Evaluation – Total spots The enzyme-linked immunospot (Elispot) 1600 IFNγ IL-2 Overlay 1400 IFNγ (single) assay detects soluble proteins secreted from 1200 Double (IFNγ + IL-2) single cells, on a membrane, using antibodies. 1000 IL-2 (single) 800 Elispot assay sensitivity, ease of use, and 600 Clear

Spots Per Well Per Spots 400 cost make these ideal multifunctional T cell 200 0 assays for the research, therapeutic, and Clear Black White Clear Black White Clear Black White Unstim (250k) CEF (250k) Anti-CD3 (50k) diagnostic communities. While Elispot assays Plate Format/Cell Stimulation (Cell #) permit frequency determination for very rare events, data interpretation can become Black ambiguous when spot numbers in antigen- Figure 1. Dual color IFNg/IL-2 fluorescent containing wells are low or spot counts in Elispot (FluoroSpot) assays were performed on total PBMC in three different Multiscreen®HTS negative control wells are elevated. Thus, it is plate formats (Clear, Black, and White). Spot clarity was roughly equivalent on the clear and important to optimize basic assay parameters White and reagents to maximize the signal-to-noise black formats. By contrast, white plates showed Cell high background signal, making spot detection ratio. Unstimulated IFNγ difficult, particularly in the Green channel (IFNg- Clear Black White FITC). A comparison of spot counts demonstrated

a significant reduction in “spots counted” on Analyze These parameters include white plates when compared to either black or (but are not limited to): clear formats.

Choice of Plate (Membrane): PVDF membrane plates are recommended over a mixed cellulose ester format, due to slightly improved binding of capture Plate Organization/Edge Effects: Coating Plates with Capture Antibody: To antibody and superior performance in Medium evaporation from peripheral wells ensure performance while maximizing cost spot detection, particularly for fluorescent in prolonged cultures may impact overall efficiency, it is critical to standardize the applications. MultiScreen® plates for Elispot assay performance. Where possible, the use amount of capture antibody used per well. For feature multiple advantages, including low of “media only” wells around the periphery optimal performance, we recommend initial background, lot-to-lot reproducibility, uniform of the true sample wells can minimize this titration of both the coating and detection spot distribution, automation compatibility effect. antibodies in tandem. and a removable underdrain for membrane access. Prewetting: Prewetting the membrane is not universally Negative/Positive Controls: applicable to all Elispots; its requirement is Negative controls routinely consist of cells dependent on the inherent hydrophobicity cultured without stimuli, whereas polyclonal of the capture antibody; therefore, the pre- T-cell activators are commonly used as wetting protocol should be optimized prior to positive controls to confirm both cell and application. assay functionality.

For more information, including ordering information for MultiScreen® Elispot plates, visit: www.merckmillipore.com/elispot www.merckmillipore.com 37 Analyze Cell 38 wholeorganismFor screening MultiScreen®-Mesh Filter Plates with 96-Well Receiver Plate For and measure the migration ofthe organisms. into the wells, treat with target compound, nematodes orother multicellularorganisms organisms. Simply transfer parasites, cytotoxicity, anddeath ofmulticellular system for assays measuring paralysis, tray fit together to provide a ready-to-use 96-well format. Thefilter plate and96-well for compound andtarget identificationina MultiScreen®-Mesh filter plate isoptimized whole, multicellularorganisms. The evaluate ofnew the compounds effect on target screening andother applicationsthat in aone-step protocol. Thesystem isidealfor for culturing, testing and reading assay results This filter plate provides acomplete system MultiScreen®-MESH Description Ordering Information Filter Plate Materials Specifications MultiScreen® MultiScreen® Single-well Culture Tray MultiScreen® 96-well Culture Tray

information

96-well

Plate

Transport

visit:

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Nylon mesh Polystyrene Receiver

Plate Pore Size (µm) Sterility Qty/Pk Catalogue No. Catalogue Qty/Pk Sterility Pore Size (µm) 0 N 100 20 40 60 NA NA NA Whole Applications • • Superior • Automation-compatible • Chemical • Multiple &Benefits Features analysis imaging micr Plates collection

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are o-trl 10 Non-sterile o-trl 10 Non-sterile o-trl 10 Non-sterile o-trl 50 Non-sterile trl 10 Sterile trl 10 Sterile

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compatibility optically oscope orother microscopic

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filtration

clear

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and

use applications MANM10010 MANMN2010 MANMN4010 MANMN6010 MATRNPS50 MAMCS0110 MAMCS9610

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with

Product Highlight

® MultiScreen HTS Vacuum Manifold Crosstalk-free for superior reproducibility

The MultiScreen®HTS Vacuum Manifold is The MultiScreen®HTS Vacuum Manifold is also DirectStack™ Technology Improves designed to improve filter-based assay ideally suited for automation. The compact Assay Reliability performance and reliability. The manifold size of the manifold base is modeled on ANSI/ supports a wide variety of MultiScreen® SBS standards for microplates to fit most platforms, including 96-well and 384-well robot deck locations. The manifold collar filter plates for bioassays and deep-well is lightweight and features a groove for 1 Solvinert™ filter plates for sample preparation. easy handling by robotic gripper systems. If additional precision is needed for placement The manifold configuration is easily adapted of the collar during assembly/disassembly 2 to accommodate filter-to-waste or collection routines, a collar holder accessory is available. 3 assays. For those assays where filtrate collection is required, the MultiScreen® • DirectStack™ technology enables crosstalk- HTS 4 Vacuum Manifold incorporates DirectStack™ free filtrate collection technology. This feature eliminates gaps • Unique design removes hanging droplets, between flow directors and receiver wells to eliminating cross-contamination risk Cell increase assay reliability and eliminate cross • Configurations for deep-well or standard 1. Manifold Collar receiver plates ® talk. The direct stacking of plates also makes 2. MultiScreen HTS Filter Plate vacuum initiation effortless. • ANSI/SBS-compliant footprint allows for 3. Collection Plate Analyze easy robotic deck integration 4. Manifold Base • Solvent-resistant Figure 1. Plate-on-plate stacking eliminates gaps between flow directors and receiver wells in applications that require filtrate collection. Featured Application Notes and Technical Notes Download from: www.merckmillipore.com

MultiScreen®HTS Filter Plates: New Plate Design for Full Automation Compatibility Data Sheet; Lit No. PF1544EN00

High throughput MultiScreen®HTS filter plates are specifically developed for use with automation. The 96-well plates are designed to standardized dimensions and meet ANSI/ SBS 2004 plate specifications. They are fully compatible with automated gripper arms, stackers, bar code labels, and plate readers.

MultiScreen®HTS Vacuum Manifold Data Sheet; Lit No. PF2014EN00

For MultiScreen®HTS Vacuum Manifold ordering information, see page 40.

www.merckmillipore.com 39 Analyze Cell 40 For vacuumFor filtration of96and384samples MultiScreen® Ordering Information Deep Adapters for MultiScreen® MultiScreen® MultiScreen® Description Solvinert, Solvinert DeepWell Solvinert Solvinert, and MultiScreen® HighVolume Filter Plates Includes manifold base,standard collar, gaskets, gasket alltubing,valves, inserts, andpressure gauge High Volume Collar Collar Holder, for automation

Well

Collar HTS HTS Vacuum Manifold for Usewith 96-and 384-well MultiScreen® Vacuum Manifold HTS Vacuum Manifold HTS Vacuum Manifold Product Performance f incorporates The MultiScreen® collection accommodate filter-to-waste orfiltrate configuration iseasily adapted to automated ormanualprotocols. Themanifold to solvents for andsuitable usewith MultiScr filtr f the 96- and384-well filter plates as well as per DirectStack™ eature eliminates gaps between flow amilies. Manifold components are resistant

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and Microfluidic Cells

(Hippocampal/Cortical),

Yeast Qty/Pk Catalogue No. Catalogue Qty/Pk EV262 1 GM230 1 MIC230 1 5 5 5 5 5 5 5 1 1 1 1

Plates) (S. cerevisiae, B04A-03-5PK C04A-01-5PK M04G-02-5PK M04L-03-5PK M04S-03-5PK Y04C-02-5PK Y04D-02-5PK ABN2 ABI1 F84-GL3-GK F84-HG3-GK

Application Spotlight

Studying drug-induced cell death of 3D cell cultures using the CellASIC® ONIX Platform

Summary The main objective in cell culture is eliciting conditions required for healthy cell culture, within the CellASIC® ONIX Microfluidic cellular responses that match physiological an application-specific microfluidic plate and Chambers. The CellASIC® ONIX system was responses as closely as possible. Current manifold with recirculating heater. The plate then programmed to follow a schedule of strategies for 3D cell culture include growing and manifold were kept on the microscope, continuous medium flow throughout the cells in a natural or synthetic 3D matrix, on enabling live imaging of the cell culture. experiment. After 96 h, two inlet wells were biodegradable polymers, in a cross-linked replaced with medium containing different hydrogel or in porous synthetic scaffolds Static culture. MCF7 cells in Matrigel® matrix concentrations of doxorubicin, and the consisting of interconnected voids in a were added to the wells of a Millicell® EZ slide protocol was resumed for another 48 h. woven mesh. However, even these advanced chambered slide. Once the matrix solidified, cell culture platforms, if subjected to static complete medium was added to each well. Automated, in-plate viability staining. The conditions of gas, nutrient medium and waste Cells were then incubated at 37 °C. LIVE/DEAD® Viability Kit (Life Technologies) buildup, may dry out and are limited by the was prepared according to the manufacturer’s

inefficient mass transport between the inside Automated microfluidic perfusion culture protocol. In this assay, live cells, stained Cell and outside of 3D cell structures. and drug treatment. MCF7 cells were with Calcein-AM, fluoresce green, and dead suspended in ice-cold Matrigel® matrix cells, stained with EthD-1, fluoresce red. The To overcome the challenges of mass transport Analyze in 3D culture, we used a microfluidic platform Imaging Window Air Channels to achieve perfusion-based microenvironment control and study drug-induced cell death of A 3D cultures of MCF7 cells. We compared these results against a traditional chambered slide. B 5.2 mm Compared to traditional slides, the C microfluidic platform provided better imaging results and consistency of cell culture, while D maintaining similar cell morphology and 1 5432 6 7 8 viability. In addition, microfluidics enables continuous media flow in and out of a 3D Cell Chamber Marker culture, increasing the efficiency with which Perfusion cells can access nutrients and eliminate Barriers waste. This setup increases the longevity Gravity Inlet (1) of 3D culture and allows one to design Flow Inlets (2,3) Cell Culture Chamber Waste experiments to study dynamic cell responses Cell Inlet (6) (2.8 mm diameter) (7-8) to drug over time. Flow Inlets (4,5) Gravity Inlet (1) Materials and Methods The CellASIC® ONIX Microfluidic platform is composed of a microfluidic system, which delivers a controlled perfusion of the cells, Figure 1. The microfluidic plate contains four independent flow units (A-D), each with four upstream solution inlets, a gravity flow inlet, a cell inlet, and two waste a microincubator controller, which provides wells. Continuous flow of solutions from inlets 2-5 can create a dynamic exposure the temperature and gas microenvironmental profile for imaging responses of live, 3D cultures. www.merckmillipore.com 45 Application Spotlight

plate was sealed to the manifold and placed static culture in the chambered slide (Figure on the lab bench, and a two-step protocol 2B, 2D). Because cell cluster growth was was created in order to flow live/dead stain, limited by the height of the microfluidic plate followed by PBS, into the cell chambers. chambers, more cells were in sharp focus in the same focal plane than were in focus in Results the chambered slide. Long-term 3D culture. MCF7 cells formed cell clusters after 24 h that maintained their Drug-induced cell death. Cell viability was morphology after 96 h of culture in both assessed using in-plate live/dead staining. We perfusion culture (Figure 2A, 2C) and in observed the expected increase in red staining with respect to increasing doxorubicin dose, indicating that the 3D cell clusters were being Microfluidic plate Chambered slide permeated by the drug as well as by the staining reagents (Figure 3). A. B. Discussion 24 h We have shown that it is possible to simultaneously maintain four, virtually hands-free, healthy, 3D cell cultures for several days using perfusion feeding. Further, we analyzed drug-induced cell death using C. D. hands-free, in-plate viability staining. This demonstrates that this platform enables the Cell analysis of the response of individual cells 96 h within 3D culture over time. In addition, the microfluidic platform enables the culture of a larger number of cell clusters per focal plane,

Analyze providing better imaging results. The ability to analyze more cell clusters at once, in parallel Figure 2. 3D cell cultures in sharp focus using the microfluidic platform (A,C). Static cultures in chambered slides (B,D) showed similar experiments, may provide increased statistical morphology but fewer cell clusters per focal plane. Scale bars = 100 μm. power to studies of cellular response and enable the routine use of 3D cultures for higher-throughput studies and screening. 200 µM Doxorubicin 20 µM Doxorubicin

100 µM Doxorubicin NO DRUG

Figure 3. Dose-dependent MCF7 cell death upon doxorubicin For product ordering information, treatment as measured by in-plate live/dead staining of 3D cultures. see page 43. Scale bars = 100 μm.

46 www.merckmillipore.com Product Highlight

Simple, scalable cellular analysis solutions: Muse® Cell Analyzer and guava easyCyte™

Plot P01: Ungated FlowPlot P04:Cytometers. Gated on CD3- cells 4 10

You’ll quickly3 find that guava easyCyte™ microcapillary flow cytometers are simpler to operate 10 and maintain than traditional sheath fluid-based instruments. They all use small sample 6000 800070009000 2 50004000300020001000 volumes,10 generate minimal waste and have lower operating costs, enabling on-demand use in any lab environment. Equipped with insightful data acquisition and analysis software, guava 1 CD19 APC (RD2-HLog) 10 Side Scatter-(SSC-HLin) easyCyte™ instruments are available with single-load and high throughput options. 0 0

100 101 102 103 104 10 100 101 102 103 104 CD45 PerCP-Cy5.5 (RED-HLog) ImmunologicalCD16+56 PhenotypingPE (YLW-HLog) 6-Color Assay

Plot P02: Gated on CD45+ cells Plot P03: GatedPlot onP01: CD3+ Ungated cells Plot P04: Gated on CD3- cells 4 4 10 10 3 3 10 10 CD3+ & CD4+ 6000 800070009000 6000 800070009000 B-Cells 2 2 50004000300020001000 50004000300020001000 10 10 CD3+ & CD8+ 1 1

CD19 APC (RD2-HLog) NK Cells CD4 PE-Cy7 (NIR-HLog) 10 10 Side Scatter-(SSC-HLin) Side Scatter-(SSC-HLin) Cell 0 0 0 0 10 10 0 1 2 3 4 0 1 2 3 4 10 10 10 10 10 10 10 10 10 10 100 101 102 103 104 100 101 102 103 104 CD3 FITC (GRN-HLog) CD8CD45 APC-Cy7 PerCP-Cy5.5 (NI2-HLog) (RED-HLog) CD16+56 PE (YLW-HLog) Analyze Plot P02: Gated on CD45+ cells Plot P03: Gated on CD3+ cells

50 μL adult human blood was stained for 15 minutes at room4 temperature with a cocktail containing CD3-FITC, CD16-PE, CD56-PE, CD45-PerCP-Cy5.5,10 CD4-PE-Cy7, CD19-APC, and

CD8-APC-Cy7, lysed, fixed and analyzed on a guava easyCyte™3 8HT system. 10 6000 800070009000 2 50004000300020001000 Learn more about guava easyCyte™ flow cytometers at:www.merckmillipore.com/guava 10 1 CD4 PE-Cy7 (NIR-HLog) 10 Side Scatter-(SSC-HLin) 0 0

With the Muse® Cell Analyzer, we’ve miniaturized flow cytometry10 and packed 3-parameter 0 1 2 3 4 10 10 10 10 10 100 101 102 103 104 analysis into a compact,CD3 FITC affordable (GRN-HLog) instrument, bringing accurate CD8cell APC-Cy7 analysis (NI2-HLog) to every benchtop. Effortlessly acquire data with our optimized Muse® assays designed for immunology, cell health, apoptosis, and cell signaling studies.

Muse® Annexin V & Dead Cell Assay

Untreated Gambogic acid Apoptotic impact of gambogic acid on Jurkat cells.

For product ordering information, see page 43.

Learn more about the Muse® Cell Analyzer at: www.merckmillipore.com/muse

www.merckmillipore.com 47 Product Highlight

Cytometry without limits: Extend the range of your research

The Amnis® FlowSight® and ImageStream®X Mark II cyometers combine the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.

Autophagy During autophagy, cytoplasmic LC3 is processed and recruited to the outer membrane of autophagosomes. Cells undergoing autophagy can be identified by visualizing LC3 puncta and enumerating the spots within each cell using the Spot Count feature of the IDEAS software package:

Spot Count Scores

Cell 3 5 10 33

Figure 1. The IDEAS image processing software included with the ImageStream®X Mark II cytometer determines the Spot Count of every cell. In this example, cells with varying number of LC3-FITC spots are shown with their corresponding Spot Count. Analyze Example: Autophagy in the Human CML Cell Line K562

Control Untreated

Figure 2. K562 cells were treated with etoposide to induce autophagy. Representative brightfield and merged LC3-FITC (green) and DAPI (purple) images are shown for control and treated cells. The number of LC3 puncta was quantified for each cell using the Spot Count feature of the IDEAS Etoposide-Treated software and each sample of over 10,000 cells was characterized by a Spot Count histogram. The percentage of cells exhibiting one or more puncta increased from 13% (control) to 44% (etoposide-treated).

For complete product information, visit: www.merckmillipore.com/amnis

48 www.merckmillipore.com Whether you need to clone a gene, modify a DNA sequence, quantify Genomic intracellular DNA and RNA or express a recombinant protein, you need a complete set of genomic analysis tools that work together. Building on Sample the molecular biology expertise of Novagen, Merck Millipore’s products support every step of your genomic Preparation analysis workflow. Learn how our tools for RNA detection, PCR, DNA purification, cloning, transfection and protein expression can help you develop smarter, more predictive model systems for your research.

Prepare/Clone Transfect/Express Detect

Successful gene discovery starts with high-fidelity PCR, high- recovery DNA purification, efficient DNA propagation and library page 57 page 61 preparation. Our 100% guaranteed polymerases, quality reagents, kits and competent cell platforms help overcome specific challenges in DNA amplification and propagation. Prepare/Clone Genomics 50 application requirements. polymeraseDNA system basedonyour PCR enzymes.Choosethe appropriate KOD PCRreactions ascomparedtypical to other polymerase systems extendthe limitsof to amplifyability longPCRtargets, KOD GC-rich sequences. Combinedwith their crude samples andtargets with upto 90% in-class amplification for awidearray of The KOD polymerase systems provide best- full-length product infewer reaction cycles. for higheryieldsof and highprocessivity low frequency, mutation extensionrate, fast thermostable polymerase DNA that provides KOD polymerase DNA isanultra high-fidelity, Optimized for the most challenging samples orDNAtemplates Novagen® KOD Polymerase PCRSystems Note: NovaTaq™ polymerase isalsoavailable for routine PCR. *KOD-XL polymerase DNA amplification results inamixture ofbluntand3’-dAproducts while other polymerasesKOD DNA generate bluntendproducts. Applications PCR Product Size (kb) Specifications O N oyeaeKDHtSatDAPlmrs KOD XLDNA*Polymerase KOD Hot Start DNAPolymerase KOD DNAPolymerase amplification Cloning, cDNA <6 lnn,cN mlfcto Crude samples, incorporation multiplex, amplification Cloning, cDNA <21 • Efficient • High &Benefits Features • Amplification • Optimized For moreFor information visit: www.merckmillipore.com/kod Product Performance NovaTaq™ NovaTaq™ Polymerase DNA K KOD KOD KOD KOD Polymerase DNA Description Ordering Information OD XL DNA PolymeraseOD XLDNA GC-r minimal sam com 24 kb polymerases

Hot Xtreme™ Hot pared to most other proofreading DNA

ich templates accuracy,

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Expression Polymerase KOD Xtreme™ Hot Start DNA and GC-rich targets Crude samples, longtargets, difficult <40 www.merckmillipore.com

Analysis ,0 71003-5 71003-4 2,500 U 71003-3 500 U 100 U 71087-4 71087-3 1,250 U 250 U 71086-4 71086-3 1,000 U 71086-5 200 U 71085-3 20 U 250 U 0 x 71842-4 500 rxn ,5 71091-4 71091-3 1,250 U 250 U 71975-3 200 U 100 r Qty/Pk Catalogue No. Catalogue Qty/Pk n71842-3 xn Application Spotlight

High recovery genomic DNA concentration using the new Microcon® PCR-grade centrifugal ultrafilter

Maximum recovery of pure intact genomic 0.50 Figure 1. Equally fast ultrafiltration DNA from crude biological samples is 0.45 flow of Microcon® DNA Fast Flow the critical first step in any DNA analysis 0.40 (Orange) and Microcon® DNA Fast 0.35 workflow. Typically, the process involves cell 0.30 Flow PCR Grade (Yellow) devices as 0.25 determined by measuring retentate disruption, chemical or ion exchange-based 0.20 volume at various time intervals and extraction of the nucleic acid fraction and 0.15 plotting retentate volume vs. centrifu- 0.10 gation time. Each point represents the concentration of the purified DNA sample. 0.05 mean and standard deviation of four While ethanol precipitation is commonly used Avg. Retentate Sample Volume (mL) 0 0 2 4 6 8 10 12 14 replicates. for DNA concentration in research settings, an Centrifuge Time @ 500 x g (min.) effective alternative is ultrafiltration via small spin columns such as the Microcon® DNA Fast A Genomics Flow device. However, one concern in genetic 100% Figure 2. DNA recovery performance 90% of Microcon® DNA Fast Flow (Orange) testing is consumable product contamination 80% and Microcon® DNA Fast Flow PCR with exogenous DNA during assembly and 70% 60% grade (Yellow) Microcon® devices as packaging. To minimize this potential, we have 50% determined by comparison of retentate released the Microcon® DNA Fast Flow PCR 40% volume to starting material. Results 30%

show percent genomic DNA recovered Prepare/Clone

Grade filter, which is ethylene oxide-treated to % Genomic DNA Recovery 20% with respect to centrifugation step. In disrupt or fragment amplifiable DNA. Here, we 10% 0% each case, the starting material was show that it matches the original Microcon® Initial 1 Wash 2 Washes 3 Washes either PCI-extracted DNA (A) or DNA Centrifugation Step filter in performance. in TE Buffer (B). For PCI samples, three successive wash steps were performed. B 100% Each bar represents the mean and stan- 90% dard deviation of four replicates. 80% 70% 60% 50% 40% 30%

% Genomic DNA Recovery 20% 10% 0% Initial 1 Wash Centrifugation Step

10.0 Figure 3. No inhibition of qPCR reactions. 1159 bp DNA qPCR average 7.5 threshold cycle (Ct) results for Micro- 5.0 con® DNA Fast Flow and Microcon® Avg. Ct DNA Fast Flow PCR Grade devices using 2.5 pLH2 template DNA (1 ng). The qPCR results for the unprocessed PCR Tube 0 Control 1159bp results (1 ng and

1 ng 10 ng) are also shown. Each bar 10 ng represents the mean and standard For Microcon® ordering information, deviation of four replicates. Template DNA Template DNA please refer to page 100. 2x EtO-treated Cat. #MCRF0R100 1 ng Template DNA 1 ng Template DNA Cat. #MCRF0R100ET

Microcon® DNA PCR Tube Controls Fast Flow Filters www.merckmillipore.com 51 Prepare/Clone Genomics 52 Remove primers andunincorporated dNTPsinonestep PCR CleanupFilter Plates MultiScreen® PCR MultiScreen® PCR Filter Plate MultiScreen® PCR Filter Plate 1159 bp:71 ng/µL 657 bp:55 ng/µL 301 bp:30 ng/µL 137 bp:10 ng/µL usingthe obtained following used.Data concentrations: concentration, load,andbuffers basedonstarting *Results willvary ++ Best Recovery + GoodRecovery -- Not Recommended Filter Plate MultiScreen® PCR Product name MultiScreen® PCR Filter Plate MultiScreen® PCR Product name Specifications Filter Plate Filter Plate 384 96 µ96 384 96 µ96

010µ 96 20–150 µL 5–0 L96 150–300 µL 010µ 384 20–100 µL ecinvlm ubro ape oeo prto eomne plctosFeatures Recommended applications Modeofoperation Number ofsamples Reaction volume + -- + PCR fragment* of137bp Recovery ++ + ++ PCR fragment* of301bp Recovery MultiScreen®PCR use reagents andsave money. volumes >150µL, For reactions. Working volumes <150µLconserve recovery oneven the smallest miniaturized handling the with plate liquid enhancescompatibility required. Full sample recovery from the top of results. No centrifugation orprecipitation is to provide aone-step protocol with excellent exclusion membrane andvacuumfiltration volumes, formats andaµ96-well format for small purification. Available in96-and384-well solutions for high-throughput PCR Our PCRfilter plates arefast, automatable

MultiScreen®PCR

MultiScreen® systems. Vacuum Vacuum Vacuum ++ ++ ++ PCR fragment* of657bp Recovery 384

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a and and ++ ++ ++ fragment* of1159bpPCR Recovery

size- • MultiScreen® • • No • High &Benefits Features Pur Microarray, PCR Cleanup,Sequencing, Genotyping, Applications com Fast (1-150 bp) recommended for smallfragments ification, Genomic DNA Concentrationification, GenomicDNA

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RNAi www.merckmillipore.com • • • • • • 99.8% 98.7% 99.5% volume % Primer removal (20bases),100 µL

and Reaction Highly Volumetric Ultra-high Reaction Highly

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for protein

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venience tabilizing target plasmids tabilizing target S Insoluble protein/no activity Insoluble protein/no Target Application Target Protein labeling Protein Protein induction from T7 induction from Protein vectors expression activity Insoluble protein/no Truncated protein Truncated No protein/cell death due to toxic toxic due to death protein/cell No protein target pecific applications con s • Easy-to-use Applications Bacterial Features & Benefits • High • Selection

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10 1 10 1 10 S384PCR10 50 50 1 WP6111560 50 S384PCR50 1 WP6122050 1 WP6110060 L volumes to to L volumes express

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and reliability, Singles™ Competent Cells Cells Competent Singles™ and reliability, in 50 μ provided are partially used vials. or waste thaw, minimizes time and money, This saves reliable contamination and ensures cell performance. eliminate the need to aliquot, freeze/ aliquot, need to the eliminate polymerase

BLR(DE3) 150–300 1 20–150 1 20–100 Reaction Volume (µL) Volume Reaction Qty/Pk Catalogue No. 150–300 20–150 1 20–100 1 1

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Feature A methionine auxotroph ideal for protein labeling applications protein ideal for auxotroph A methionine Lysogens of bacteriophage λDE3 of bacteriophage Lysogens misfolding LacY basal e Express expression protein full-length improved tRNAs for rare Supply RecA proteases that minimize protein degradation protein minimize that proteases Deficient in Lon and OmpT expression protein General TrxB/gor Hz

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B834 Strain Expression Strains Expression DE3 Hosts Application Guide Required Equiment Required MultiScreen® MultiScreen® Ordering Information Ordering Description Filter Plates Filter Tuner™ and Rosetta-gami™ B and Rosetta-gami™ Tuner™ Strains MultiScreen® Chemical (DE3) pLysS Hosts (DE3) pLysS and Rosetta-gami™ Rosetta Strains MultiScreen® MultiScreen® Chemical HMS174 and NovaBlue Strains HMS174 and NovaBlue BL21 Strains Chemical Origami™ 2 and Rosetta-gami™ 2 and Rosetta-gami™ Origami™ Strains www.merckmillipore.com , you need to obtain high yields of need to E. coli, you bacterial Our soluble protein. full-length, include the expression protein for strains and specialty strains all-purpose best all proteins, difficult-to-express for strains support to technical unwavering by backed success. For convenience ensure ultimate Optimized for high yields of full-length, soluble protein soluble high yields of full-length, for Optimized in proteins recombinant When expressing Novagen® Competent Cells for Bacterial Protein Expression Protein Bacterial for Cells Competent Novagen® For more information visit: www.merckmillipore.com/PCR visit: For information more Prepare/Clone Genomics 54 moreFor information visit: www.merckmillipore.com/compcells HT96™ NovaBlue GigaSingles™ Competent Cells NovaBlue(DE3) HMS174(DE3) Rosetta Rosetta Origami BL21(DE3) NovaBlue Singles™ Competent Cells Select Competent Cellsfor Cloning BL21(DE3) Select Competent Cellsfor Bacterial Protein Expression Description Ordering Information

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Cells >1.0 x10 >1.0 x10 >1.5 x10 >5 x10 >2 x10 >2 x10 >2 x10 >2 x10 >1.5 x10 >2 x10 Transformation Efficiency 6 6 6 6 7 7 cfu/µg cfu/µg cfu/µg cfu/µg cfu/µg cfu/µg 9 8 8 8 cfu/µg cfu/µg cfu/µg cfu/µg 50 µL 50 µL 6x2 L1pae71011-3 1plate 20 µL 96 x20µL 20 µL 50 µL 50 µL 50 µL 50 µL 50 µL ecinSz Qty/Pk Reaction Size www.merckmillipore.com 2rn71227-4 71227-3 70181-4 22 rxn 70181-3 11 rxn 22 rxn 11 rxn . L69284-3 71011-4 1 mL 0.4 mL 4 plates . L69453-3 71401-4 1 mL 71401-3 0.4 mL 71400-4 22 rxn 71400-3 11 rxn 71408-4 22 rxn 71408-3 11 rxn 70236-4 22 rxn 70236-3 11 rxn 70235-4 22 rxn 70235-3 11 rxn 22 rxn 11 rxn 69284-4 69453-4 Catalogue No.Catalogue Genomics Prepare/Clone 55

BAC

LSKPMRN30 SCGVU01RE MSVMHTS00 MSNUPSD50 MSNANLY10 MSNANLY50

reproducibility

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1 bottle 1 bottle LSKCTB500 1 WP6110060 1 bottle LSKNF0500 1 WP6122050 1 bottle LSKNS0500 1 WP6111560 100 LSKAST100 1 bottle LSKCLS500 100 LSKVBP100 12 1 bottle LSKCRS500 Qty/Pk Catalogue No. 424 LSKP09604 LSKP09624 1 50

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adio-sterilized RNase A,RNase 0.9 mL Tris Buffer, 0.5 L Buffer, Tris Chemical Nuclease-free Water, 0.5 L Water, Nuclease-free Chemical Neutralization Solution, 0.5 L Neutralization Chemical Adhesive Tape Covers Tape Adhesive Cell Lysis Solution, 0.5 L Cell Lysis Plasmid Miniprep Kits and Filter Plates Kits and Filter Plasmid Miniprep MultiScreen® V-bottom plates V-bottom MultiScreen® Reagents Solution, 0.5 L Cell Resuspension Required Equiment Required MultiScreen® Stericup®-GV r Accessories Montage® Description Ordering Information Ordering www.merckmillipore.com Simply clear, concentrate, wash, and recover wash, concentrate, clear, Simply The 96-Well Plasmid and BAC Preparation Kit Preparation BAC and Plasmid 96-Well a fast, easy-to-use kit for high-purity for kit a fast, easy-to-use chromosome artificial plasmid or bacterial protocol This simple minipreps. (BAC) methods bind/elute lengthy eliminates yield clean to steps and centrifugation DNA in 50% less time and reproducible PCR Purified methods. traditional than sensitive most the suitable for are samples including cloning, applications downstream and transformation. DNA sequencing, include sequencing, applications BAC and PCR arraying, fingerprinting, reagents the all The kit includes amplification. purify need to you materials and disposable format. DNA in a 96-well plasmid or BAC Prepare/Clone Genomics 56 moreFor information visit: www.merckmillipore.com/mobio inhighly TA-processivity orGC-rich regions. unique polymerase amplifies with DNA high Hot Start Polymerase DNA Master Mix.This amplification usingourultra-high fidelity KOD preparation procedure isfollowed by library Preparation System. Theeasy, two-step library PureGenome™ High Efficiency NGS Library libraries with lesshands-ontimewith the Prepare Generation Sequencing Next (NGS) Less bias.time.More matched reads. Novagen® Preparation Reagents PureGenome™ HighEfficiency NGS Library *Kit was optimized qPCRmastermix SYBR® usingSABiosciences Validator Kit* PureGenome™ Generation Sequencing Next Library ValidationLibrary reagent boxes. The PureGenome™ reagents sets are validated together anduniquely lot-controlled to ensure preparation. library highquality Merck Millipore doesnot recommend purchasing individual Modifier DNA PureGenome™ NGS Library Preparation Library PureGenome™ On-Spot Solutionisprovided for selectingtargeted sectionareas prior to Proteinase Kdigestion andpurification. PureGenome™ Extraction DNA Kit Tissue Genomic DNAExtraction Description Ordering Information PureGenome™ NGS Library A-TailPureGenome™ NGS Library Enhancer PureGenome™ On-Spot Kit DNA Tissue PureGenome™ NGS Library AmplifierPureGenome™ NGS Library Product Performance • Easy-to-use, &Benefits Features with minimalbiasfor better results. yieldsfrom lowermaximum library input DNA, and accuracy ofamplification enables construction The combinedefficiencyoflibrary PureGenome™ Validator Primer Mix. Kit components include(3)PureGenome™ NGS Controls DNA and componentsBlunt-ending, A-tailing &ligation SpinPrep™ Columnsand Collection Tubes.buffers, PureGenome™ optimized Kitscontain Proteinase Kfor tissuelysis, Components PureGenome™ NovaTaq Polymerase for A-tailing SpinPrep™ Columnsand Collection Tubes.buffers, PureGenome™ optimized Kitscontain Proteinase Kfor tissuelysis, amplification KOD Hot Start Polymerase DNA Mastermix enrichment/ for library 2-s tep workflow

less

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Genomic Sample Preparation

Prepare/Clone Transfect/Express Detect Choose the genes you express, the cells you use and the proteins you purify to answer your biological questions; don’t let limitations page 49 like toxicity, solubility or yield page 61 influence your choice of system. Our guaranteed transfection reagents, vectors and competent cells help tackle even the most intractable genes and gene products. Transfect/Express Genomics • Compatible • Highly &Benefits Features • Simplified • Minimal 58 moreFor information visit: www.merckmillipore.com/transfection complex formation with DEAE-dextran. microinjection, delivery, biolistic particle and phosphate coprecipitation, electroporation, techniques includinglipofection, calcium alternative to ofother awidevariety This transfection reagent isasuperior cytotoxicity for successfulgene expression. efficiency, easeofuse,andminimal optimized for maximaltransfection GeneJuice® Transfection Reagent is High-efficiency transfection ofcells for awide variety Novagen® GeneJuice®Transfection Reagent eeuc®Taseto egn Thestandard for transfection inmost celllinesinserum-containing orserum-free GeneJuice® Transfection Reagent Description ProteoJuice™ RiboJuice™ Insect GeneJuice®Transfection Reagent NanoJuice® 293-Free™ Transfection Reagent NovaCHOice® Other Transfection Reagents *The 1mLsize provides enoughreagent to perform up to 500transfections instandard 35mmplates. GeneJuice®Transfection Reagent issuppliedasaready-to-use sterile solution. Ordering Information t and ser s ypes andapplications table andtransient transfectionstable

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Ov Ov Overnight Overnight Overnight Ov *EasyPak Ordering information Ordering Description Overnight www.merckmillipore.com For more information visit: www.merckmillipore.com/OvernightExpress visit: For information more Applications Bacterial • High Features & Benefits • Effortless • Convenient convenience, allowing you to focus on your on your focus to you allowing convenience, its job. while it does research in The sim Sy or Bacterial expression without monitoring or manual IPTG induction IPTG or manual monitoring expression without Bacterial expression protein bacterial Increase Novagen® Overnight Express™ Autoinduction Systems Autoinduction Express™ Overnight Novagen® density. culture monitor to having without The Transfect/Express Genomics 60 transgene inthe chromosome. into protein, regardless ofthe positionofthe maximizing itspotential to betranscribed environment around anintegrated transgene, tr activ house-k DNA t the and highgene expression, irrespective of silencing andgiv (UC Ubiquitous cells by manipulating chromatin structure. expression instably transfected mammalian Achieve dramatically improved gene Rapid, high-yield protein production inmammaliancells MammalianGeneExpression TechnologyUCOE® echnology expression elementsare small anscriptionally active openchromatin

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Prepare/Clone Transfect/Express Detect Accurately detect nucleic acids in your samples with our well- published, well-documented agarose and electrophoresis reagents. page 49 page 57 Our molecular biology-grade and Omnipur® grade reagents are DNAse-, RNAse- and protease-free to give you peace of mind and reproducible results. Detect Genomics 62 applications. and cellculture work aswell asother sensitive RNase, andproteases for safe useintissue reagents are tested for the absenceofDNase, performance from lot to lot. OmniPur®grade anddeliver consistent highest quality molecular biologyreagents that are ofthe OmniPur® products represent agrade of DNAse-, RNAse-andprotease-free reagents Calbiochem® OmniPur®Grade Biochemicals andReagents

• DNase-, &Benefits Features For moreFor information visit: www.merckmillipore.com/OmniPur • Suitable • Stringent OmniPur® OmniPur® OmniPur® OmniPur® Description Ordering information Note: ourwebsite Visit for acomplete listing ofOmniPur®offerings. OmniPur® F f lot-to-lot best results acilities

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OmniPur® Agarose, low melting low OmniPur® Agarose, OmniPur® Agarose, high gel strength high gel OmniPur® Agarose, OmniPur® Related Products Related OmniPur® Agarose Description OmniPur® Ordering information Ordering o prevent smearing or high fluorescence or high fluorescence smearing o prevent www.merckmillipore.com For more information visit: www.merckmillipore.com/OmniPur visit: For information more t average gel strength and standard melting melting and standard strength gel average and OmniPur® Superior resolution of DNA fragments resolution Superior of a grade represent OmniPur® products of are that reagents molecular biology quality highest consistent and deliver the grade OmniPur® lot. to lot from performance DNase, absence of the for tested are reagents use in tissue and safe for and protease RNase, applications. cell culture Calbiochem® OmniPur® Grade Agarose PCR Plus PCR Agarose Grade OmniPur® Calbiochem® Application Spotlight

A novel reagent enables live cell RNA detection and enhances sorting capabilities

Detecting gene expression has traditionally products. SmartFlare™ technology enables to sort, but it also improves sorting accuracy been limited to technologies that examine users to sort cells based on the level of by using biologically relevant intracellular expression in lysed or fixed cell populations. specific RNAs with high recovery, providing a markers. As shown in Figure 2, SmartFlare™ The ability to detect RNAs in individual, live new opportunity to study cellular functions probes were successfully used to sort a mixed cells can enable an unequivocal assessment and identify rare cell types such as certain cell population based on miRNA expression, of gene expression, changes that occur in tumor cells and cancer stem cells. Not only and sort products could then be tested for direct response to specified perturbations. does this technology facilitate the sorting of functional differences. Determining which genes are up- or down- cell populations that were previously difficult regulated in these perturbed cells provides

insight into the relationships between gene In absenseShort ofDuplexes target, probe TargetTarget (mRNA)When target is present it willLonger bind Duplex Formed expression networks and cell functions. We is quenchedShortFlares Duplexes byare golddark. and does Target (mRNA) the capture strand,Longer Duplex releasing FormedFlare released. Flaresnot are dark.fluoresce the reporter strandFlare released.and fluorescing developed SmartFlare™ RNA Detection Probes, capable of detecting specific mRNAs and miRNAs in live, intact cells (Figure 1). This technology allows for carrier-free cellular endocytosis of the reagent, followed by + + detection and relative quantitative analysis of

Genomics RNA levels.

Achieve multiparametric, predictive cell analysis with no sample prep,

Detect cell lysis or toxicity. The oligo duplexes are designed with

Because the SmartFlare™ probe leaves the cell -AAAAAAATCAACCATACACCGTGACTTTGCTTGACCC Capture Strand an RNA “capture” sequence and a complementary “reporter” sequence. after the detection event, the same sample -AGTTGGTATGTGGCACT Reporter Strand -AAAAAAATCAACCATACACCGTGACTTTGCTTGACCC Capture Strand can be used for any downstream analysis, -AGTTGGTATGTGGCACT Reporter Strand enabling the measurement of multiple Figure 1. Molecular mechanism of SmartFlare™ detection probes. biomarkers or downstream functionalities in the same cells. Additionally, this reagent requires no upfront sample preparation, has A. B. no toxic effects on cellular fate and no known 250 80 Plot P02, gated on P01.R1 miR155 Low 70 miR155 High nonspecific, off-target effects. Compared 204 60 FC = 8.7 to currently used methods for interrogating 153 50 RNA that involve examination of non- 40 102 Count 30 native, amplified RNA targets, SmartFlare™ FSC-Height 51 20 probes can provide results that show greater 10 correlation to in vivo observations. 0 0 100 101 102 103 104 105 100 101 102 103 104 mIR-155 Expression Level VCAM-FITC (GRN-HLog) Sort cells based on intracellular (Cy5 Fluorescence) gene expression. Separating cells based on intracellular markers Figure 2. (A) Sorting based on miR-155 expression level differences in HeLa and HUVEC cells. typically requires fixing or permeabilizing (B) Overlay of TNFα-stimulated miR-155 low and high sort products showed an 8.7 fold difference in cells, resulting in poor recovery of viable sort VCAM expression, as expected, because HeLa cells lack TNFα receptors.

64 www.merckmillipore.com Application Spotlight

Measure RNAi-mediated knockdown In contrast, SmartFlare™ RNA Detection Probes in live cells detect RNA at single cell resolution (Figure Detecting changes in gene expression 3), thereby providing information on cell-to- in individual, live cells is crucial when cell variations in expression, knockdown and performing RNAi-mediated gene knockdown efficiency of siRNA entry. Such information may studies, in which it has traditionally been greatly facilitate the interpretation of analyses difficult to determine the cause of incomplete performed subsequent to RNAi treatment. knockdown. Traditional methods of RNA detection (which measure average RNA Also, SmartFlare™ technology makes it possible levels) cannot distinguish between inefficient to sort cells and use them in downstream knockdown due to a poorly designed siRNA analyses, such as immunocytochemistry, flow sequence, inefficient entry of the siRNA into cytometry and xenografts, potentially increasing target cells, or vast differences in endogenous the strength of observed correlations between gene expression within the target cells. gene expression and cell phenotype.

Plot P02, gated on P01.R1 60 Figure 3. SCC12 cells 50 Survivin mRNA levels detected in live, siRNA-treated SCC12 cells Genomics 40 (yellow) using SmartFlare™ probes and flow cytometry reveals a 30 bimodal distribution of survivin Count expression. 20

10 Detect

0 100 101 102 103 104 Red2 Fluorescence (RED2-HLog)

No probe Survivin SmartFlare™ probe w/Survivin siRNA Survivin SmartFlare™ probe w/Control siRNA

Endless possibilities, countless applications. We and our customers are discovering the virtually limitless potential of SmartFlare™ probes. Our ever-expanding catalogue of ready-to-order probes include mRNAs and miRNAs relevant to cancer, development, signaling, epigenetics, neuroscience and more. We’ve used them for simultaneous detection of multiple RNAs, as well as for detection of both nucleic acids and proteins in the same live cells, providing links between the transcriptome and the proteome that were missing until now.

Visit our website to view more performance data, browse our complete selection of ready-to-order SmartFlare™ probes, or design your own. www.merckmillipore.com/smartflare

www.merckmillipore.com 65 Application Spotlight

Four steps to better chromatin immunoprecipitation

Chromatin immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA

complexes. Specific antibodies enrich for 1 2 3 4 5 6 7 8 9 10 11 12 regions of chromatin that contain the protein of interest, and various detection methods are employed to detect specific DNA sequences within the enriched chromatin.

Accurate, reproducible ChIP results start with high quality chromatin and require robust and specific antibodies, reliable controls, H4 1-19 K12ac and approaches that provide high levels of enrichment with low backgrounds. With an R&D group focused on ChIP innovation and improvement, Merck Millipore has developed Rabbit IgG the products for optimizing every step:

Genomics Specificity screening of anti-Acetyl Histone H4 (Lys12) using AbSurance™ Step 1. Start with high-quality arrays. The Histone H2A, H2B, H4 Array (Cat. No. 16-665) was probed with anti-acetyl histone H4 (Lys12) antibody (1:2000 dilution, Cat. No. 04-119). chromatin specifically optimized Peptides were visualized using a donkey anti-rabbit IgG, peroxidase-conjugated, for ChIP. H+L secondary antibody (Cat. No. AP182P) and a chemiluminescence detection PureEpi™ Chromatin Preparation and system. Signal was only detected in replicate spots containing the target peptide Detect Optimization Kit: Discover the first kit and rabbit IgG positive control. specifically designed to produce high-quality chromatin for ChIP experiments. The PureEpi™ approach is detailed in the kit protocol, which addresses multiple chromatin isolation and Step 3. Verify specificity of your new Magna ChIP® HiSens Kits deliver high fragmentation parameters that impact ChIP antibody. signal and low noise from thousands of cells— performance. AbSurance™ Histone Antibody Specificity or a million. Engineered to outperform any Unlike Merck Millipore, not all competing kit, and with proven performance Step 2. Select the best performing Arrays: suppliers rigorously evaluate the specificity of for a range of sample types and amounts, antibodies and controls proven their antibodies. This simple dot blot approach the Magna ChIP® HiSens Kit may likely be the for ChIP. allows any lab to evaluate histone antibody only ChIP kit you’ll ever need. ChIPAb+™ Antibodies: Get more than just specificity in advance of critical experiments. standard ChIP antibodies with ChIPAb+™ See the complete selection of kits, assay, kits. All ChIPAb+™ antibodies are rigorously Step 4. Use a flexible and antibodies for chromatin, DNA validated in-house and quality controlled in immunoprecipitation approach that methylation and RNA analysis at: ChIP, and include a matched negative control gives high levels of enrichment and www.merckmillipore.com/epigenetics IgG antibody and a set of qPCR primers to a low background. positive locus for guaranteed success in ChIP. Magna ChIP® HiSens Kit: This specialized A/G blend of Magna ChIP® beads and advanced SCW buffer system included in the

66 www.merckmillipore.com High purity and recovery mean better discovery—so start with the Protein best tools for protein purification and preparation. Instead of arduous protocols, focus your efforts on exciting proteome analyses. From Sample protein extraction and protein purification, to protein concentration and desalting, we facilitate every step Preparation of your sample preparation workflow with ultrafiltration devices, magnetic beads, extraction kits and more.

Extract Purify Optimize/ Quantify/Detect Concentrate Choosing the right protein extraction protocol can depend on your sample type and your protein analyte(s) of interest. That’s why we offer a complete, page 79 page 93 page 111 diverse range of reagents and enzymes for gentle, efficient cell lysis and protein extraction, preserving the integrity and activity of your target protein. Protein Extraction Reagents Application Guide

Starting Applications Material Analysis

Product Total Culture Cell Pellet HT Compatible 1D PAGE 2D PAGE IEF MS Western Blot Activity Assay Purification Comments E. coli BugBuster® Protein 4 4 4 4 4 4 4 Efficient protein extraction fromE. coli under non- Extraction Reagent denaturing conditions. Extraction enhanced by the addition of rLysozyme™ Solution and Benzonase® Nuclease. Can be used on cell pellets from any size culture. BugBuster® HT 4 4 4 4 4 4 4 4 Rapid protein extraction and nucleic acid degradation. Protein Extraction Ideal for processing many samples of any volume. Reagent Benzonase® Nuclease is premixed in the lysis reagent. Extraction enhanced by the addition of rLysozyme™ Solution. BugBuster® 4 4 4 4 4 4 4 4 BugBuster® Master Mix combines BugBuster® Protein Master Mix Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution. Convenient, all-in-one protein extraction reagent efficiently lyses bacteria and digests nucleic acids. BugBuster® 4 4 4 4 4 4 4 Ideal as an extraction method for purifying metal- (primary amine- dependent proteins or proteins to be used for free) Extraction immobilization or crosslinking. Extraction enhanced by Reagent the addition of rLysozyme™ Solution and Benzonase® Nuclease. BugBuster® 10X 4 4 4 4 4 4 4 A concentrated form of BugBuster® Protein Extraction Protein Extraction Reagent. Ideal for extraction when a specific buffer is

Protein Reagent required for protein stability. Extraction enhanced by the addition of rLysozyme™ Solution and Benzonase® Nuclease. PopCulture® 4 4 4 4 4 4 Protein extraction from cells directly in the culture Reagent medium; no centrifugation required. Designed for small volumes. Extraction enhanced by the addition of Extract rLysozyme™ Solution and Benzonase® Nuclease. Yeast YeastBuster™ 4 4 4 4 4 Efficient protein extraction from yeast under Protein Extraction non-denaturing conditions from any volume of Reagent culture. Add 0.5 M THP Solution (included) and Benzonase® Nuclease for enhanced efficiency. Insect CytoBuster™ Protein 4 4 4 4 4 4 4 Gentle lysis of insect cells with retention of protein Extraction Reagent activity for assays and purification. Can use with + ** ** monolayers or pellets derived from suspension cultures. Reportasol™ 4 4 4 4 4 4 4 Optimized for maximal activity of reporter enzymes Extraction Buffer (b-gal, firefly, andRenilla luciferases). Passive lysis of + ** ** R monolayers. Insect PopCulture® 4 4 4 4 4 4 Lysis of insect cells directly in serum-free medium. Reagent Ideal for expression screening of many small samples. Compatible with affinity purification.

Key: 1D PAGE = One-dimensional Polyacrylamide Gel Electrophoresis + = Cell pellet or adherent cells MS = Mass Spectrometry * = SDS must be removed before IEF R = Reporter Assay IEF = Isoelectric Focusing G = Gel Shift 2D PAGE = Two-dimensional Polyacrylamide Gel Electrophoresis ** = Salt must be removed before IEF

68 www.merckmillipore.com Protein Extraction Reagents Application Guide

Starting Applications Material Analysis

Product Total Culture Cell Pellet HT Compatible 1D PAGE 2D PAGE IEF MS Western Blot Activity Assay Purification Comments Mammalian CytoBuster™ Protein 4 4 4 4 4 4 4 Gentle lysis of mammalian cells with retention of Extraction Reagent protein activity for assays and purification. Can use + ** ** with monolayers or pellets derived from suspension cultures. Reportasol™ 4 4 4 4 4 4 4 Optimized for maximal activity of reporter enzymes Extraction Buffer (b-gal, firefly, andRenilla luciferases). Passive lysis of + ** ** adherent cells. PhosphoSafe™ 4 4 4 4 4 4 4 4 4 Ideal for extraction of phosphorylated proteins. Extraction Reagent + ** ** NucBuster™ Protein 4 4 4 4 4 4 4 Rapid isolation of nuclear protein fraction from Extraction Kit mammalian cells. Ideal for electrophoretic mobility G ** ** shift assays. ProteoExtract® 4 4 4 4 4 4 Total proteome extracted into one fraction. Mammalian Complete Proteome Extraction Kit * * * ProteoExtract® Transmembrane 4 4 4 4 4 4 4 Enables mild and efficient extraction of Protein Extraction Kit transmembrane proteins such as GPCRs.

ProteoExtract® 4 4 4 4 4 4 4 Produces four native protein fractions Protein Subcellular Proteome based on subcellular localization. Extraction Kit (*) (*) (*) ProteoExtract® 4 4 4 4 4 4 4 Produces two native protein fractions, Native Membrane membrane and non-membrane. Proteome Extraction Kit Extract Lysis and Extraction Enhancement Gram- rLysozyme™ 4 4 4 4 4 4 4 Cleaves bond in peptidoglycan layer of negative Solution E. coli cell wall. Use alone or combined with bacteria BugBuster® or PopCulture® reagents for improved (E. coli) protein extraction. Use with Benzonase® Nuclease to reduce sample viscosity and degrade nucleic acids. Lysonase™ 4 4 4 4 4 4 4 Convenient mixture of rLysozyme™ Solution and Bioprocessing Benzonase® Nuclease minimizes pipetting steps. Reagent Gram-positive Chicken 4 4 4 4 4 4 4 Cleaves bond in peptidoglycan layer of bacteria Egg White bacterial cell wall. Lysozyme Solution All cells Benzonase® 4 4 4 4 4 4 4 Degrades all types of nucleic acids Nuclease for more efficient protein extraction, faster chromotagraphy, and reduced interference in assays.

www.merckmillipore.com 69 Application Spotlight

Automated purification of proteins from non-clarified lysate

We developed a one-step lysis protocol using Panel A. Traditional recombinant protein purification workflow with mechanical lysis and clarification. BugBuster® Master Mix to gently disrupt the HARVEST LYSIS CLARIFICATION PURIFICATION E. coli cell wall while simultaneously reducing • Home made buffer lysate viscosity. Subsequently, PureProteome™ systems Pellet culture by Centrifugation PureProteome™ Total Time • Add lysozyme, protease ~185 minutes Nickel Magnetic Beads could be used to purify centrifugation inhibitors and endoprotease Nickel Magnetic Beads (largely hands-on time) recombinant His-tagged proteins without • Sonication lysate clarification. This “condensed” purifica- • Freeze/Thaw cycles tion workflow can be automated on systems ~120 minutes ~20 minutes ~45 minutes such as the KingFisher® particle processors, providing fast and reproducible results. Panel B. Integrated lysis and purification of non-clarified lysate with magnetic beads.

• Reproducible results with minimal HARVEST LYSIS PURIFICATION hands-on time

• Significant time savings with high yields Pellet culture by • BugBuster® Master Mix PureProteome™ Total Time centrifugation lysis reagent Nickel Magnetic ~70 minutes compared to the traditional workflow • Add protease inhibitors Beads (15 minutes • Magnetic beads eliminate the need to clarify hands-on time) lysates by centrifugation Protein • Automatable on platforms such as the ~25 minutes ~45 minutes KingFisher® particle processor Figure 1. One-step lysate preparation without clarification (Panel B) saves considerable time compared to traditional recombinant protein purification, which requires manual lysis and lysate clarification (Panel A). Extract Learn more at: www.merckmillipore.com/pureproteome

35 Figure 2. BugBuster® Master 30 Mix generates higher yields 25 of total protein upon 20 purification. Automated processing generated yields 15 comparable to manual 10 µg of Total Protein processing, and omitting 5 the clarification step 0 provided similar yields. 0 Clarified Lysate Clarified Non-Claarifirifieedd NNon-on-CCllarifiearifiedd BugBuster® Lysate Lysaate BugBBugBusteuster® Lysate

Manual KingFisher® Duo System

For BugBuster® Master Mix ordering information, please refer to page 71. For EDTA-free inhibitor cocktail ordering information, please refer to page 76.

70 www.merckmillipore.com Protein Extract

Catalogue No. 71456-3 71456-4 70584-3 70584-4 70794-3 BugBuster® other using or

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Extract Purify Optimize/ Quantify/Detect Concentrate Reduce sample complexity to better understand protein function, using our solutions for affinity purification, protein- page 67 protein interaction page 93 page 111 studies and albumin/ IgG depletion. Our beads, proteomics kits and Amicon® Pro purification system help reduce background while maintaining high recovery. Purify Protein 80 Product Performance Purify, exchange andconcentrate inonedevice buffer Amicon® Pro Purification System single spin. exchange a highly efficient buffer (>99%)in for elution,concentration and simultaneous Simply attach the includedAmicon®Ultra filter the needfor multiplecentrifugation steps. a range ofsample volumes, the device reduces alargeFeaturing that reservoir accommodates exchange.sample concentration andbuffer spin columnpurification with downstream system. Thedevice combinesaffinity-based in the all-in-oneAmicon®Pro purification wash, elute and/orconcentrate your protein and reduce hands-on timewhenyou bind, Avoid the risks associated with sample transfer process with many steps andmultipledevices. Traditional protein purification isalong

• Fast: • Gentle: • High &Benefits Features R Desalting, Proteins, Protein Enrichment, Exchange, Buffer Purification, Depletion ofAffinity Abundant Applications • Flexible: eactions fit ar buf exchange continuous flo sam fer ofyour choice, injust 5spins ple loss

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Simultaneous lysis and capture using the Amicon® Pro system expedites purification of bacterially expressed recombinant proteins

The standard protein purification workflow Traditional Lysis/Sonication Gravity Column Buffer Exchange ~122 min involves extraction, affinity-based capture, Method + Clariﬁcation Puriﬁcation + Concentration and sample optimization (Figure 1). Traditional mechanical lysis methods are tedious and BugBuster® Reagent Lyse / Purify / Exchange Buffer / Concentrate harsh, leading to diminished protein integrity + Amicon® Pro Device ~69 min and prep-to-prep variability. Gravity- Resuspend pellet in BugBuster® Master Mix. driven agarose columns are frequently Transfer to Amicon® Pro Device with Ni-NTA resin. used for affinity purification. While easy to Lyse, bind, wash, elute, exchange buffer manipulate, columns may clog due to debris and concentrate, all in the Amicon® Pro Device. or high lysate viscosity, so necessitating the clarification of the lysate before it is added to Figure 1. Condensing the recombinant protein purification workflow. Compared to traditional purification involving mechanical lysis (sonication), affinity purification using gravity columns, plus the column. Moreover, final optimization of additional devices for final sample formulation, less time is required for combined chemical lysis/capture/ buffer composition and protein concentration sample optimization, using BugBuster® Master Mix and the Amicon® Pro system. requires a separate device, increasing the

Protein risk of sample loss. Here, we demonstrate a Lyse then Capture Lyse and Capture condensed workflow, combining the bacterial Rep A Rep B Rep A Rep B cell lysis and affinity capture steps (Figure 1). This is made possible by the gentle, detergent- Std Lys 1 2 3 1 2 3 1 2 3 1 2 3 10 mL Culture

Purify based BugBuster® Master Mix lysis reagent, which includes Benzonase® nuclease for Lyse then Capture Lyse and Capture reducing lysate viscosity, and the Amicon® M Ly FT W E FT W E FT W E FT W E Pro purification system, which directly links affinity-based spin column purification with buffer exchange and concentration. His-GAPDH

Following resuspension of bacterial cell pellets in BugBuster® Master Mix, all steps Figure 2. Combined lysis and capture maintains protein yield and purity. For “Lyse then Capture”, a were performed within the Amicon® Pro cell pellet was resuspended in BugBuster® Master Mix, lysed by agitation, clarified by centrifugation device. Cell pellets were converted to purified, and purified in the Amicon® Pro device. For “Lyse and Capture,” a replicate pellet was resuspended in concentrated protein in the correct buffer BugBuster® Master Mix and then mixed with resin in the Amicon® Pro device without clarification. Fractions were analyzed by SDS-PAGE. Lanes: Std - molecular weight standard, Lys - lysate pre-passage, for downstream application. This workflow 1 - flowthrough, 2 – wash fraction, and 3 – elution fraction. requires fewer process steps and less total time without sacrificing yield or sample purity (Figures 2 and 3). Confining the sample to 6.E+07 Figure 3. Relative protein recovery in the a single device reduces loss and minimizes 5.E+07 respective elution fractions was determined inter-prep variation. 4.E+07 by gel densitometry. For each method, the bar represents the average of four individual 3.E+07 replicate purifications. On average, for a 10 For Amicon® Pro System ordering 2.E+07 mL pellet, “Lyse and Capture” resulted in information, see page 80. approximately 10% greater his-GAPDH yield Gel Band Intensity 1.E+07 than the “Lyse then Capture” method. For BugBuster® Master Mix ordering 0.E+00 information, see page 71. Lyse then Capture Lyse and Capture

82 www.merckmillipore.com Protein Purify 83

ligand purification

for LSK2ABG20 LSK2ABA20 Catalogue No. 16-266 16-125 16-156

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Recommended for Amicon® Pro Description Size System Supplied As: Binding Capacity Application Cat No. Protein A Agarose 1.5 mL + 30% slurry Use 15 µL suspension/ IP: pre-blocked with BSA. Not for IP02-1.5ML Suspension µg antibody purification Protein A Agarose 10 mL ++ 50% slurry 20 ± 2 mg human IP; affinity purification 16-125 IgG/mL settled agarose Protein A Agarose 10 mL + 50% slurry; highly cross- 40 mg human IgG/mL Medium and low pressure 16-156 Fast Flow linked 6% agarose beads agarose chromatography (flow rates 50 - 300 cm/h) Protein G Agarose 10 mL ++ 50% slurry; highly cross- 20 mg human IgG/mL Medium and low pressure 16-266 Fast Flow linked 4% agarose beads agarose chromatography (flow rates 50 - 300 cm/h) Protein G Plus 1.5 mL + 30% slurry Use 15 µL suspension/ IP: pre-blocked with BSA. Not for IP04-1.5ML Agarose Suspension µg antibody purification Protein G Plus/ 1.5 mL + 30% slurry Use 15 µL suspension/ IP: pre-blocked with BSA. Not for IP05-1.5ML Protein A µg antibody purification Suspension Protein G Plus/ 10 mL + 50% slurry Use 5-10 mL of packed Antibody purification IP10-10ML Protein A Agarose beads per mL serum Streptavidin 10 mL + 50% slurry 1.5 to 2.5 mg/mL of IP; column or batch purification of 16-126 Agarose biotinylated rabbit IgG biotinylated molecules Streptavidin 5 mL + 50% slurry > 85 nmol free IP; column or batch purification of 69203-3 Agarose biotin/mL biotinylated molecules His•Bind® Resin 10 mL + Uncharged IDA agarose 8 mg/mL bed volume Uncharged resin: User can charge with 69670-3 50 mL + resin metal ion of choice. Small to medium 69670-4 100 mL + scale purifications using either gravity 69670-5 flow columns or batch method

His•Bind® 1 kit + Separate vials of: Bind, n/a Solutions are included for Ni2+ 69755-3 Buffer Kit Wash, Elution, Stripping, charging, binding, washing and elution and Charging Buffers of up to ten 2.5 mL columns

Protein His•Bind® 1 kit (comes with • 10 mL His•Bind® Resin 8 mg/mL bed volume Small scale purifications using gravity 70239-3 Purification Kit chromatography • 1 His•Bind® Buffer Kit flow columns columns) • pkg/4 Chromatography Columns Ni-NTA 10 mL ++ 50% slurry 5–10 mg His•Tag® One-step gravity flow purification 70666-3

Purify His•Bind® Resin 25 mL ++ fusion protein per mL of proteins containing a His•Tag® 70666-4 100 mL ++ resin sequence 70666-5 Ni-NTA His•Bind® 10 mL + 50% slurry Compatible with FPLC 70691-3 Superflow™ Resin 25 mL + 70691-4 100 mL + 70691-5 BugBuster® 1 kit (comes with Separate vials of: 5–10 mg His•Tag® Gentle lysis of E. coli to release soluble 70751-3 Ni-NTA His•Bind® chromatography BugBuster® Protein fusion protein per mL protein and one-step gravity flow Purification Kit columns) Extraction Reagent; resin purification of proteins containing a Benzonase® Nuclease, His•Tag® sequence purity >90%; Ni-NTA His•Bind® Resin; Chromatography Columns BugBuster® 1 kit (comes with Separate vials of: 8 mg/mL bed volume Gentle lysis of E. coli to release soluble 70793-3 His•Bind® chromatography BugBuster® Protein protein and one-step gravity flow Purification Kit columns) Extraction Reagent; purification of proteins containing a Benzonase® Nuclease, His•Tag® sequence Purity >90%; His•Bind® Resin; His•Bind® Buffer Kit; Chromatography Columns Ni-NTA Buffer Kit 1 kit ++ Separate vials of: Ni-NTA n/a Set of buffers optimized for 70899-3 Bind Buffer, Ni-NTA Wash purification of His•Tag® fusion Buffer, Ni-NTA Elute Buffer proteins on Ni-NTA His•Bind® Resin. These phosphate-buffered solutions differ from the Tris-based solutions used in the His•Bind® Buffer Kit

n Tested n Recommended, but not tested

84 www.merckmillipore.com Agarose Selector Guide

Recommended Recommended for Amicon® Pro for Amicon® Pro Description Size System Supplied As: Binding Capacity Application Cat No. Description Size System Supplied As: Binding Capacity Application Cat No. Protein A Agarose 1.5 mL + 30% slurry Use 15 µL suspension/ IP: pre-blocked with BSA. Not for IP02-1.5ML GST•Bind™ Resin 10 mL ++ 50% slurry 5–8 mg GST•Tag™ Column or batch format purification 70541-3 Suspension µg antibody purification fusion protein per of recombinant glutathione Protein A Agarose 10 mL ++ 50% slurry 20 ± 2 mg human IP; affinity purification 16-125 1 mL settled resin S-transferase (GST) fusion proteins or IgG/mL settled agarose native glutathione S-transferase or Protein A Agarose 10 mL + 50% slurry; highly cross- 40 mg human IgG/mL Medium and low pressure 16-156 glutathione-binding proteins Fast Flow linked 6% agarose beads agarose chromatography (flow rates 50 - 300 cm/h) 50 mL ++ 70541-4 Protein G Agarose 10 mL ++ 50% slurry; highly cross- 20 mg human IgG/mL Medium and low pressure 16-266 25 mL ++ 70541-5 1 kit (comes with : Gentle lysis of to release 70794-3 Fast Flow linked 4% agarose beads agarose chromatography (flow rates 50 - 300 BugBuster® Separate vials of E. coli cm/h) GST•Bind™ chromatography BugBuster® Protein soluble protein and purification Protein G Plus 1.5 mL + 30% slurry Use 15 µL suspension/ IP: pre-blocked with BSA. Not for IP04-1.5ML Purification Kit columns) Extraction Reagent; of recombinant glutathione Agarose Suspension µg antibody purification Benzonase® Nuclease; S-transferase (GST) fusion proteins or Protein G Plus/ 1.5 mL + 30% slurry Use 15 µL suspension/ IP: pre-blocked with BSA. Not for IP05-1.5ML GST•Bind™ Resin; native glutathione S-transferase or Protein A µg antibody purification Chromatography Columns; glutathione-binding proteins Suspension GST Bind/Wash and Protein G Plus/ 10 mL + 50% slurry Use 5-10 mL of packed Antibody purification IP10-10ML Reconsititution Buffers Protein A Agarose beads per mL serum Glutathione, Reduced Streptavidin 10 mL + 50% slurry 1.5 to 2.5 mg/mL of IP; column or batch purification of 16-126 GST•Bind™ 1 kit ++ Separate vials of: n/a Set of pretested buffers for binding, 70534-3 Agarose biotinylated rabbit IgG biotinylated molecules Buffer Kit GST Bind/Wash Buffer; washing and elution of GST•Tag fusion Streptavidin 5 mL + 50% slurry > 85 nmol free IP; column or batch purification of 69203-3 Glutathione Reconstitution proteins from GST•Bind™ Resin or Agarose biotin/mL biotinylated molecules Buffer; Glutathione, GST•Mag™ Agarose Beads His•Bind® Resin 10 mL + Uncharged IDA agarose 8 mg/mL bed volume Uncharged resin: User can charge with 69670-3 Reduced 50 mL + resin metal ion of choice. Small to medium 69670-4 Strep•Tactin 2 mL + 50% slurry 50-100 nmol/mL settled Low pressure and FPLC 71592-3 100 mL + scale purifications using either gravity 69670-5 Superflow Agarose 10 mL + resin, or up to 3 mg of chromatography 71592-4 flow columns or batch method 30 kDa protein per mL settled resin Protein His•Bind® 1 kit + Separate vials of: Bind, n/a Solutions are included for Ni2+ 69755-3 Strep•Tactin 1 kit + Separate vials of: n/a Pretested buffers for use with 71613-3 Buffer Kit Wash, Elution, Stripping, charging, binding, washing and elution Buffer Kit Strep•Tactin Wash, Elution, Strep•Tactin Resins to purity and Charging Buffers of up to ten 2.5 mL columns and Regeneration Buffers Strep•Tag® II fusion proteins His•Bind® 1 kit (comes with • 10 mL His•Bind® Resin 8 mg/mL bed volume Small scale purifications using gravity 70239-3 Strep•Tactin 1 kit (prepacked mini • 25 Strep•Tactin Each column purifies up Purification of Strep•Tag® II fusion 71608-3 Purification Kit chromatography • 1 His•Bind® Buffer Kit flow columns SpinPrep™ Kit spin columns) SpinPrep™ Columns and to 150 µg of Strep•Tag® proteins using mini spin columns

columns) • pkg/4 Chromatography Collection Tubes II fusion protein Purify Columns • Separate vials of Ni-NTA 10 mL ++ 50% slurry 5–10 mg His•Tag® One-step gravity flow purification 70666-3 Strep•Tactin Wash Buffer His•Bind® Resin 25 mL ++ fusion protein per mL of proteins containing a His•Tag® 70666-4 and Elution Buffer 100 mL ++ resin sequence 70666-5 D-Desthiobiotin 1 g + lyophilized powder n/a Gentle elution of Strep•Tag® II 71610-3 Ni-NTA His•Bind® 10 mL + 50% slurry Compatible with FPLC 70691-3 proteins from the biotin-binding site of Superflow™ Resin 25 mL + 70691-4 Strep•Tactin® resins 100 mL + 70691-5 T7•Tag® Affinity 1 kit (comes with Separate vials of: T7•Tag® > 300 µg T7•Tag® Rapid immunoaffinity purification of 69025-3 BugBuster® 1 kit (comes with Separate vials of: 5–10 mg His•Tag® Gentle lysis of E. coli to release soluble 70751-3 Purification Kit chromatography Antibody Agarose; T7•Tag® β-galactosidase per mL target proteins that carry the T7•Tag® Ni-NTA His•Bind® chromatography BugBuster® Protein fusion protein per mL protein and one-step gravity flow columns) Bind/Wash Buffer, Elution of settled resin sequence (i.e., the amino terminal 11 Purification Kit columns) Extraction Reagent; resin purification of proteins containing a Buffer, and Neutralization aa of the T7 gene 10 protein) Benzonase® Nuclease, His•Tag® sequence Buffer; Chromatography purity >90%; Ni-NTA Column His•Bind® Resin; T7•Tag® Antibody 2 mL + 50% slurry 69026-3 Chromatography Columns Agarose BugBuster® 1 kit (comes with Separate vials of: 8 mg/mL bed volume Gentle lysis of E. coli to release soluble 70793-3 S-Protein Agarose 2 mL + 50% slurry The capacity varies and Purification of S•Tag™ 69704-3 His•Bind® chromatography BugBuster® Protein protein and one-step gravity flow 10 mL + is based on the size and fusion proteins 69704-4 Purification Kit columns) Extraction Reagent; purification of proteins containing a S•Tag™ Thrombin 1 kit (comes with spin Separate vials of: folding characteristics 69232-3 Benzonase® Nuclease, His•Tag® sequence Purification Kit columns) S-protein Agarose; Bind/ of a given target protein Purity >90%; His•Bind® Wash Buffer; Thrombin Resin; His•Bind® Buffer Kit; Cleavage Buffer; Chromatography Columns Biotinylated Thrombin; Ni-NTA Buffer Kit 1 kit ++ Separate vials of: Ni-NTA n/a Set of buffers optimized for 70899-3 Streptavidin Agarose; Spin Bind Buffer, Ni-NTA Wash purification of His•Tag® fusion Filters Buffer, Ni-NTA Elute Buffer proteins on Ni-NTA His•Bind® Resin. S•Tag™ rEK 1 kit (comes with spin Separate vials of: 69065-3 These phosphate-buffered solutions Purification Kit columns) S-protein Agarose; differ from the Tris-based solutions Bind/Wash Buffer; rEK used in the His•Bind® Buffer Kit Dilution/Storage Buffer; Recombinant Enterokinase; EKapture™ Agarose; Spin Filters

n Tested n Recommended, but not tested www.merckmillipore.com 85 Purify Protein 86 mammalian systems. Reagents andkitsare e ar and His•Tag®, purification offusionproteins containing pr number purificationvectors bya enablesaffinity usingappropriateof fusiontags expression recombinantFor proteins, the addition His•Tag®, GST•Tag, S•Tag™, Strep•Tag II,andT7•Tag® Agarose Purification of Recombinant Fusion Proteins xpressed inbacterial, yeast, insect,or BugBuster® Ni-NTA Ni-NTA Ni-NTA BugBuster® His•Bind® His•Bind® His•Bind® His-Tag Purification Description Ordering Information e oducts specifically designed for the rapid

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Buffer SpinPrep™ Superflow Agarose GST•Bind™

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S•Tag™ Agarose Streptavidin GST-Tag Purification GST-Tag D-DESTHIOBIOTIN T7•Tag® S•Tag™ Streptavidin Streptavidin S-Protein Agarose S-Protein S-Tag Purification S-Tag T7-Tag Purification T7-Tag T7•Tag® Strep-Tag II Purification Strep-Tag Strep•Tactin BugBuster® GST•Bind™ Strep•Tactin Strep•Tactin GST•Bind™ Ordering Information - Continued Information Ordering Description www.merckmillipore.com For Purify Protein 88 Magnetic bead-basedisolationofproteins PureProteome™ Magnetic Beads Product Performance pelle pur binding purification systems feature low, non-specific magne purification, extremely andeasyprotein fast For • High &Benefits Features no sample loss. andcompleteof buffers recovery ofbeadswith using amagnetic enablingtotal stand, removal losing sample. Magnetic beadsare isolated more bindingsites for proteins combined wit ification t, followed by careful aspiration to avoid tic beads.Thesemagnetic bead

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For www SKMAGN01 SKMAGHDKIT SKMAGS08 SKMAGA02 L LSKMAGD12 L LSKMAGS15 mL LSKMAGAG02

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10 10 2 x 1 mL2 x 1 mL LSKMAGKP02 LSKMAGLM02 2 x 1 mL10 mL LSKMAGT02 LSKMAGT10 0.5 mL4 x 0.5 mL L LSKMAGN04 10 mL LSKMAG03CBX10 10 mL LSKMAG1CBX10 10 mL LSKMAG25CBX10 1 kit 1 kit 1 1 2 x 1 mL10 mL L 2 x 1 mL LSKMAGA10 10 mL LSKMAGG02 2 LSKMAGG10 2 x 1 mL10 mL LSKMAGH02 LSKMAGH10 Qty/Pk Catalogue No. Kit

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PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ Stands Magnetic Depletion Kits Depletion Active ChemistryActive Beads PureProteome™ PureProteome™ PureProteome™ PureProteome™ PureProteome™ Ordering Information Ordering Description Conjugated Beads Conjugated PureProteome™ PureProteome™ www.merckmillipore.com Purify Protein 90 Ni-NT agarose thrombin isremoved with streptavidin clea of X and r 3C Biotinylated site-specific proteases (e.g.,Thrombin, Remove with fusiontags restriction-grade Factor Xa,Thrombin, rEnterokinase, 3C HRV Site-Specific Proteases andCleavage Capture Kits Capture Kit Thrombin Cleavage Biotinylated Thrombin Thrombin Restriction-Grade Thrombin Description Specifications ecombinant arrest™

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ag•off High Cleavage Factor Xa Xarrast™ Enterokinase EKapture™ EKapture™ Tag•off™ Tag•off™ HRV *Bulk Description Restriction-Grade Ordering Information Ordering HRV 3C Protease HRV 3C Protease Bio Thrombin Restriction-Grade Recombinant Recombinant Bovine Enterokinase Bovine Recombinant Recombinant Enterokinase Specifications - Continued Specifications Description Xa Factor Bovine Grade Restriction Restriction-Grade Xa Factor Activity rEK T Enterokinase Cleavage Cleavage Enterokinase Kit Capture Tag•off rEK Cleavage rEK Cleavage Tag•off Kit Capture Factor Xa Cleavage Xa Cleavage Factor Kit Capture www.merckmillipore.com For Purify Protein 92 by step elution. you canfractionate complex peptide mixtures Todata. simplify your even analysis further, and oligonucleotides for improved analytical femtomoles to picomolesofpeptides, proteins method for concentrating andpurifying tip pr such and purifying samples for sensitive analyses fix 0.6 The Concentrating andpurifying samples for MALDI-ToF MS PipetteZipTip® Tips ZipTip® pH Range Temperature, °C OD(top),Tip mm ID(top),Tip mm Length,Tip mm Dimensions ZipTip® C Pipette Tip Materials Specifications ZipTip® Capacity Resin Bed Pipette Tip Volumes, µL SCX Resin C Resin 4 18 ed atitsend.Itisidealfor concentrating Resin

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ZTSCXS096 ZTSCXS008 ZTC18M960 ZTC18M096 ZTC18M008 ZTC04S960 ZTC04S096 ZTC04S008 ZTC18S960 ZTC18S096 ZTC18S008

recovery Protein Sample Preparation

Extract Purify Optimize/ Quantify/Detect Concentrate Downstream protein analyses, such as activity assays or structural studies, require that the protein is in its native, page 67 page 79 soluble form, dissolved page 111 in the buffer of choice and at an appropriate concentration. Our membrane-based technologies enable fast, easy concentration, desalting and buffer exchange. Application Spotlight

Exchange buffer and concentrate protein solutions over a wide range of sample types and volumes

The limitations of traditional buffer exchange the Amicon® Pro device enables concentration Only 1 Spin methods include lengthy process times, large of a wide range of volumes as well as gentle volumes of exchange buffer, and risk of buffer exchange in a single spin. sample loss. Merck Millipore’s wide selection of sample preparation devices are designed Designed for continuous diafiltration to meets the needs of specific applications. (Figure 1), the Amicon® Pro device not only For large sample volumes or proteins that offers speed and efficiency, but also greatly may aggregate if over-concentrated, D-Tube™ reduces the likelihood of protein aggregation, dialyzers provide a faster, less tedious precipitation or loss of activity. While dialysis alternative to standard dialysis cassettes is slow and gentle, most spin-based desalting Add Continuous 1000x 1.5 mL Flow Buffer Dilution (Table 1). Amicon® Ultra 4 and 15 mL methods involve small devices and multiple Buffer Exchange centrifugal filters are perfectly suited for the rounds of buffer exchange, which can alter concentration of biomarkers from biological protein stability. The Amicon® Pro device’s Figure 1. The Amicon® Pro device enables effective desalting or buffer exchange in a single fluids with high recovery. The Amicon® large upper reservoir permits continuous spin, because it features: (1) large, 10 mL buffer

Protein Ultra 0.5 mL filter gives the best balance in buffer exchange and therefore maintains reservoir, (2) minimal spacing between the time and recovery for PCR product cleanup the sample at constant volume (and Amicon® Pro device and the attached Amicon® Ultra filter, and (3) tapered exchange tip for or protein removal prior to analytical HPLC. concentration), preserving specific activity optimal buffering. These features ensure that All Amicon® Ultra filters offer desalting (Table 1). fresh buffer (blue) is slowly but consistently capability with high sample retention. Finally, metered in, mixed with sample (green dots in yellow buffer), and expelled through the membrane (green arrows). Because this is a continuous process, the sample does not undergo concentration until buffer exchange is completed. Optimize/Concentrate Dialysis Gravity Centrifugal Diafiltration D-Tube™ 2 mL Device A Amicon® Ultra-0.5 Amicon® Pro 3 mL Cassette Dialyzer Column (0.5 mL) Filter Device % Salt removal 99.9 99.5 99.6 99.4 (3 spins) 100 (3 spins) 100 (1 spin) % Protein retention 84.1 87.5 53.3 90.0 92.2 98.4 Process Time 16 Hr 5 Hr 40 min 45-60 min * 45-60 min * 15-30 min * Specific Activity 4.2 NA 3.4 3.7 3.9 4.3 Concentration required Yes Yes Yes No No No Table 1. Comparative performance analysis of representative buffer exchange devices. Data represent the results of three independent trials. The Amicon® Pro device provided the most efficient and effective buffer exchange. Dialysis was the most time-consuming, while gravity-driven columns demonstrated significant sample loss, due to fraction pooling and sample transfer. *Process Time depends on whether initial centrifugation was required to concentrate the sample.

For Amicon® Pro System ordering information, see page 80. For Amicon® Ultra Filter ordering information, see page 98. For D-Tube™ Dialyzer ordering information, see page 95.

94 www.merckmillipore.com Protein Optimize/Concentrate 95 Catalogue No. 71712-3 71713-3

10 71748-3 1010 71512-3 71514-3 5050 71745-4 dialyzers 71746-4 96 dialyzers 1 kit 71511-3 10 71513-3 Qty/Pk 6,000-8,000 10 71746-3 MWCO www.merckmillipore.com/psp visit: Volume Volume N/A N/A 20 mL 3,50010 - 250 µL 6,000-8,000 10 96 71745-3 10 - 250 µL 12,000-14,000 N/A N/A

Racks

Racks N/A N/A

Racks N/A N/A Racks N/A N/A

Assays

information syringes

Dialysis

removal

Floating

Floating (no Floating Floating

more Gels,

and

from Protein-DNA

Description Mega Dialyzer, D-Tube96™ 6-8 kDa Mini Midi Maxi Mega D-Tube96™ Dialyzer, Dialyzer, D-Tube96™ 12-14 kDa D-Tube™ Electroelution Accessory Kit

DNase-free For Functional

RNA

and

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Filtration Capacity

Membrane Membrane Page

Device Type Orientation 0.5 mL 1 mL 2 mL 4 mL 15 mL 50 mL 70 mL 200 mL 400 mL Number Small Volume Filtration Devices Microcon® Centrifugal Filter UH ● 100 Amicon® Ultra-0.5 Centrifugal Filter UV ● 98 Amicon® Pro System UV ● 80 Ultrafree®-MC Centrifugal Filter M H ● 101 Centrifree® Centrifugal Filter UH ● 104 Medium Volume Filtration Devices Amicon® Ultra-2 Centrifugal Filter UV ● 98 Ultrafree®-CL Centrifugal Filter MH ● 101 Amicon® Ultra-4 Centrifugal Filter UV ● 98 Centriprep® Centrifugal Filter UH ● 102 Amicon® Ultra-15 Centrifugal Filter UV ● 98 Large Volume Filtration Devices 8050 Amicon® Stirred Cells UH ● 105 Centricon® Plus-70 Centrifugal Device UV ● 105 8200 Amicon® Stirred Cells UH ● 105 8400 Amicon® Stirred Cells UH ● 105

Membrane Type: (U) ultrafiltration vs. (M) microfiltration Protein Membrane Orientation: (H) horizontal vs. (V) vertical

Selecting an Ultrafiltration Membrane Ultrafiltration Membrane Nominal Molecular Weight Limit (NMWL) Application 3,000 10,000 30,000 50,000 100,000 Protein concentration ● ● ● ● ●

Optimize/Concentrate Protein purification/desalting/buffer exchange ● ● ● ● ● Desalting of column fractions ● ● ● ● ● Protein isolation from cell lysates ● ● Peptide concentration/desalting/buffer exchange ● Antibody concentration ● ● ● Virus concentration or removal ● ● Nucleic acid concentration/desalting/buffer exchange ● ● ● ● ● Oligonucleotide concentration/desalting/buffer exchange ● PCR cleanup ● ● Remove linkers prior to cloning ● ● Remove labeled, unincorporated nucleotides ● ● ● Antibody purification from hybridoma cells ● Rapid restriction mapping ● Natural product screening ● ● ● ● ● Bound vs. free drugs from serum/plasma (protein removal) ● ● Removal of unincorporated label (e.g. fluorescein) from protein ● ● ● ● ● Removal of imidazole from His-tagged fusion proteins ● ● ● ● ●

96 www.merckmillipore.com Choose the right Amicon® Ultra filter for your molecule of interest

Amicon® Ultra -0.5 Amicon® Ultra-2 Amicon® Ultra-4 Amicon® Ultra-15 Staring Volume <0.5 mL <2 mL <4 mL <15 mL Final Volume 15-20 µL 15-20 µL 50 µL 200 µL

Product Concentration Factor X25-X33 X100-X133 X80 X75 Rotor Adaptor Standard 1.5 mL Standard 15 mL Standard 15 mL Standard 50 mL Fixed-Angle (35°) Rotor 14,000 g 7,500 g 5,000 g for 100 kDa 5,000 g 1,000 g reverse spin 1,000 g reverse spin 7,500 g for all other MWCO Swinging Bucket Rotor N/A 4,000 g 4,000 g 4,000 g

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Nucleic Acid (single- & double-stranded) Protein 137-1159 bp 30,000 10 min 20 min 10 min 20 min

Applications Nanoparticles 1.5 < dia < 3 nm 3,000 30 min 60 min 40 min 40 min 3 < dia < 5 nm 10,000 15 min 40 min 15 min 20 min Optimize/Concentrate 5 < dia < 7 nm 30,000 10 min 20 min 10 min 20 min 7 < dia < 10 nm 50,000 10 min 15 min 10 min 15 min 10 nm < dia 100,000 10 min 30 min 10 min 15 min

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Concentration, Buffer Exchange,Concentration, Buffer Desalting Applications • • Disc &Benefits Features • Compatible Filter Diameter (mm) Qty/Pk Catalogue No. Catalogue Qty/Pk Filter Diameter (mm) 150 90 76 63.5 47 44.5 25 150 90 76 63.5 47 44.5 25 150 90 76 63.5 47 44.5 25 ar 76, 90and150mm to meet your separation requirements Both e available inawiderange ofpore sizes

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information Membrane, Membrane, Membrane, Membrane, Membrane, Membrane, Membrane,

Biomax® Biomax® Biomax® Biomax® Biomax® Biomax® Ordering Information - Biomax® Ultrafiltration Discs (Polyethersulfone) Ultrafiltration - Biomax® Information Ordering Description Biomax® www.merckmillipore.com For 110 www.merckmillipore.com Protein Sample Preparation

Extract Purify Optimize/ Quantify/Detect Concentrate Understand protein structure and function with tools to characterize post-translational modifications and expression control. page 67 page 79 page 93 Our protein detection platforms, including our Direct Detect® spectrometer and optimized Western blotting products, help you accurately interrogate the proteins relevant to your system. Application Spotlight

Simplified analysis of lipid or detergent content in biological samples using the IR-based Direct Detect® Spectrometer

Despite the growth in lipid research, analytical The Direct Detect® spectrometer utilizes the y = 0.04519x - 0.00679 (lipid) and methods applied for their characterization typ- C-H symmetric stretching vibrational popula- y = 0.01774x + 0.00442 (detergent). These ically involve multistep procedures, requiring tion between 2870 and 2840 cm-1 to determine equations were used by the Direct Detect® extensive sample manipulation and separation lipid or detergent content (Figure 1). The Direct software to determine the concentration of from other biomolecules, like proteins, before Detect® assay-free sample card enables analy- Tetracosanoic Acid and CHAPS in subsequent the analysis. sis of aqueous-based biological samples, which samples. are normally not compatible with infrared The infrared (IR)-based Direct Detect® spectroscopy, due to their high water content. Robustness of the Relative Absorbance spectrometer enables simultaneous protein These assay-free cards are also compatible with mode was further demonstrated through quantitation and lipid analysis, directly from many organic solvents. simultaneous analysis of protein and lipid a biological sample. Given that each lipid content in samples containing mixture of possesses an IR signature uniquely defined Materials and Methods both biomolecules as well as in breast cancer by its chemical composition and structure, IR Measurements of sample concentration were tissue lysate. Frozen tissue, derived from a spectroscopy offers a means of qualitative acquired using Direct Detect® assay-free sample breast ductal carcinoma, was divided into 2 Protein lipid discrimination, as well as quantitation of cards (Merck Millipore, Cat. No. DDAC00010-8P) equal samples. Tissue was covered with 2 mL known lipid(s) in cases where a viable standard and the Direct Detect® spectrometer (Merck RIPA buffer (Merck Millipore, Cat. No. 20-188) curve has been determined. Millipore, Cat. No. DDHW00010-WW). Each as- or CytoBuster™ protein extraction reagent say-free card contains four polytetrafluoroeth- (Merck Millipore, Cat. No. 71009-50mL), both ylene (PTFE) membrane positions, sized for easy supplemented with an inhibitor cocktail, and Symmetric Stretching -CH -(CH )- disrupted with a glass tissue homogenizer. 3 2 sample application and analysis. All measurements were performed using 2 µL of sample Effective removal of the fatty fraction from solution per membrane position. Unknown the resulting tissue homogenate by a series of Quantify/Detect lipid mixtures were analyzed in the “Relative centrifugation steps was also monitored by the Cholesterol Absorbance” mode, where the system delivers Direct Detect® spectrometer. information based solely on IR signal strength. NP40 Empirical sample concentration values were Results LPS determined by interpolation from calibration Accuracy of lipid and detergent (Lipopolysaccharide) curves developed for each specific lipid or de- quantitation tergent. For the experiments reported here, the The accuracy of concentration estimation

SDS system was calibrated using Tetracosanoic Acid within the dynamic ranges established for in chloroform and 3-[(3-cholamidopropyl)di- Tetracosanoic Acid and CHAPS was assessed Triglyceride methylammonio]-1-propanesulfonate (CHAPS) using 0.8 mg/mL Tetracosanoic Acid in 3000 2800 in phosphate-buffered saline (PBS). A series of chloroform and 1.8 % CHAPS in PBS. Within a Wavenumber (cm-1) seven concentrations from 0.25 mg/mL well-defined calibration method, the instru- to 1.75 mg/mL was used to generate an ment was capable of estimating lipid and Figure 1. C-H symmetric stretching bands observed in the infrared spectra of lipids. instrument calibration curve for Tetracosanoic detergent concentration with minimal error: Acid. For CHAPS, the calibration curve was the Direct Detect® spectrometer estimated formed using a series of seven concentration the concentration of the Tetracosanoic Acid points spanning 0.25% to 4%. The strength of samples to be 0.853 ± 0.14 mg/mL (2.4%CV) IR signal for each concentration was fitted to a and the CHAPS samples were estimated to be regression line represented by linear equations 1.8 ± 0.004% (2.3%CV).

112 www.merckmillipore.com Application Spotlight

Protein quantitation and lipid 0.04 content analysis performed using 0.04 0.03 the same measurement 1.5 0.02 0.02 IR spectral profiles of proteins and lipids are 0.01 distinct, thereby enabling the simultaneous Absorbance Units Absorbance Units 0.00 1.0 0.00 Phospholipids (0.3 AU) Proteins (2.8 mg/mL) quantitation of proteins and analysis of lipids 3000 2950 2900 2850 2800 1700 1650 1600 1550 1500 using the same measurement. Protein content Wavenumber (cm-1) Wavenumber (cm-1) can be quantified using one of the pre-loaded Absorbance Units 0.5 standard curves (all prepared in PBS pH 7.4)2. Lipid analysis can be performed, using the same spectra, in the “Relative Absorbance” 0.0 mode. We analyzed phospholipids in a complex 3500 3000 2500 2000 1500 1000 500 mixture (Figure 2). The protein in the sample Wavenumber (cm-1) was quantified at 2.8 mg/mL. Figure 2. Analysis of protein and lipid content from a single sample measurement. Because the sample Monitoring the lipid profile during composition and ratio of individual components were unknown, the analysis of the phospholipids was performed using the “Relative Absorbance” mode. the preparation of a breast cancer tissue lysate Traditionally, quantitation of proteins and lipids in lysates has been tedious, requiring large 0.20 Conclusions The Direct Detect® spectrometer enables rapid sample volumes and specialized methodol- 0.16 Protein analysis of lipids and detergents in addition to ogies, and the results for either component 0.12 accurate and reproducible protein quantitation. obtained using classical methods may be 0.08 The ability to simultaneously monitor protein obscured by cross-interference. The Direct De- concentration and fat removal during sample tect® spectrometer has enabled rapid analysis 0.04 preparation provides a tool for assay optimi- Quantify/Detect of total protein with simultaneous monitor- 0.00 A1 A2 A3 B1 B2 B3 zation as well as greater confidence in final ing of lipid content, thereby simplifying and CytoBuster™ Solution RIPA sample purity. improving the analytical process3. Breast cancer tissue lysates were prepared using RIPA buffer Figure 3. Lipid content as determined using the References and CytoBuster™ protein extraction reagent. Direct Detect® spectrometer in the “Relative 1. Pidgeon C., Apostol G. and Markovich R. Anal. Biochem. 1989, Absorbance” mode in 3 different fractions 181(1): 28-32. Following tissue homogenization, the Direct 2. Mer ck Millipore. Literature No. AB3355EN00, 2012. of breast cancer tissue lysates prepared with Detect® spectrometer was used to monitor the 3. Gutierrez, S. et al. Poster Presentation, Human Proteome Orga- CytoBuster™ Protein Extraction Reagent or RIPA nization (HUPO) 11th Annual World Congress, Boston, MA, USA, efficiency of fat removal and total protein con- buffer. September 2012. centration during centrifugal extraction. Direct Detect® spectrometer data showed gradual Total protein content removal of a fatty fraction from the samples (mg/mL) (Figure 3). The same spectra were also used to Spin condition CytoBuster™ Protein Sample & fraction collected Extraction Reagent RIPA determine the total protein recovery across the A1 spin @10,000 xg (top fatty fraction) 5.0 14.0 various fractions (Table 1). From this limited A2 spin @15,000 xg (top fatty fraction) 2.7 20.0 study, it is clear that the Direct Detect® system A3 spin @15,000 xg (bottom layer) 5.0 17.0 offers a means for in-line process optimization B1 spin @10,000 xg (top fatty fraction) 3.3 3.6 for maximal yield and/or purity. B2 spin @15,000 xg (top fatty fraction) 2.1 5.0 B3 spin @15,000 xg (bottom layer) 2.8 5.0

Table 1. Total protein recovery from breast cancer tissue lysed with RIPA buffer or CytoBuster™ protein extraction reagent measured using the Direct Detect® spectrometer.

For Direct Detect® spectrometer ordering information, see page 114. www.merckmillipore.com 113 Quantify/Detect Protein 114 IR-based proteinIR-based quantitation Direct Detect® Spectrometer Product Performance preps easierthan ever! carbohydrates—making lysates andmembrane from the AmideI&IIregion, like lipidsand components ofcomplex mixtures separable (redox) potential. You canevaluate major dye bindingproperties orreduction-oxidation without relying onaminoacidcomposition, an intrinsic component protein ofevery chains, the system accurately quantitates By measuring amidebondsinprotein without the ofcolorimetric pitfalls assays. system provides more accurate results ever? TheDirectBradford Detect® orBCA, What ifyou never hadto run another uniainApoc Assay Range Direct Detect® System Approach Quantitation Specifications Bradford Assay Micro BCA™ AssayMicro BCA™ Lowry AssayLowry 5-,0 gm 2 µL 250-5,000 µg/mL 0200µ/L50µL/25µL 20-2,000 µg/mL 2-4 µg/mL 0150µ/L200 µL/40µL 10-1,500 µg/mL • Preserves • IR &Benefits Features Quantit Protein Applications • New • More • Improves Sample Volume (tube/plate) 500 µL/150µL long aliphaticc absor quantitation across allproteins andpeptides ag color information from your sample quantitation assays, providing more that interfere with traditional protein L per analysis 2 μLperanalysis

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Direct Direct Detect® Assay-free Cards (50/pk) Cards Assay-free Detect® Direct Direct Detect® Assay-free Cards (50/pk) Cards Assay-free Detect® Direct Direct Detect® Assay-free Cards (50/pk) Cards Assay-free Detect® Direct Consumables Direct Detect® Spectrometer Detect® Direct Ordering Information Ordering Description Kit and Starter Spectrometer Detect® Direct Includes: Universal Dell Software Detect® Direct Netbook Tray Spotting Cable Ethernet (50/pk) Cards Assay-free Detect® Direct Product Performance Product www.merckmillipore.com pr The or reprobing. transfer during proteins access it easy to makes structure open pore unbound probes. and remove bound proteins In protein blotting application. Convenient application. Convenient blotting protein sheets pre-cut feature sandwiches blotting paper. filter and blotting of membrane Immobilon® Immobilon® Immobilon® Membranes, Sandwiches and Blotting Filter Paper Filter and Blotting Sandwiches Immobilon® Membranes, blotting Western for membranes PVDF transfer For in t Quantify/Detect Protein 116 For Specifications Immobilon®-FL Immobilon®-FL Membrane, 0.45 Immobilon®-P Immobilon®-P Immobilon®-P Immobilon®-P Immobilon®-P PVDFTransfer Membrane, 0.45 Description Ordering Information Goat IgG BSA Insulin Protein BindingCapacity, µg/cm Filter Pore Size, µm Filter Material Immobilon®-P Immobilon®-P Immobilon® Blotting Sandwiches Immobilon®-P

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VFHydrophobic PVDF μ μ m m 448 340 262 0.2 26 x 20 x 15 x 10 x 9 x12 8.5 x13.5 8 x10 7 x8.4 No. Catalogue Qty/Pk Filter Dimensions(cmxcm) 26.5 x375 8.5 x13.5 7 x8.4 7 X8.4 26.5 x375 20 x 10 x 8.5 x13.5 7 x8.4 26.5 x375 26 x 20 x 15 x 10 x 9 x12 8.5 x13.5 8 x10 7 x8.4

SQ PVDF ebaeImmobilon®-FLMembrane Membrane www.merckmillipore.com 300 205 155 0.45 Hydrophobic 10 10 10 10 10 10 10 50 1 20 20 10 1 10 10 100 100 1 10 10 10 10 10 10 10 50

IPVH304F0 IPVH20200 IPVH15150 IPVH10100 IPVH09120 IPVH08130 IPVH08100 IPVH07850 IPVH00010 IPSN08132 IPSN07852 IPFL07810 IPFL00010 IPFL20200 IPFL10100 IBFP0813C IBFP0785C ISEQ00010 ISEQ26260 ISEQ20200 ISEQ15150 ISEQ10100 ISEQ09120 ISEQ08130 ISEQ08100 ISEQ07850 PVDF Product Highlight

Detection: Fluorescent Westerns Immobilon®-FL transfer membrane

Immobilon® Supplier W Supplier G Supplier G FL NC PVDF PVDF-FL Fluorescence-based detection of Western blots, while increasing in popularity due to multiplex detection capabilities, requires 700 nm specialized tools to obtain optimal results. Merck Millipore’s solutions for fluorescent Westerns, including Immobilon®-FL membrane, are designed to work together for fast, reproducible protein detection. 800 nm

Publications citing Immobilon®-FL: ~9,000 Figure 1. Lowest background fluorescence. Compared to nitrocellulose (NC) and PVDF membranes from other suppliers, Immobilon®-FL membrane exhibits the lowest background fluorescence at both wavelengths. Protein How does Immobilon®-FL membrane work? Ex λ: This 0.45 µm membrane is the first transfer 473 nm Cy2

membrane specifically optimized for Quantify/Detect fluorescence-based detection of Western blots. Its extremely low background autofluorescence improves sensitivity of all 532 nm fluorescence detection protocols. Cy3

Key Benefits • The only membrane that works at near- infrared wavelengths (700-800 nm) 635 nm Cy5 • Strong signals due to higher protein adsorption & retention on the membrane • Low background to detect even faint bands • High tensile strength for multiple stripping and reprobing cycles 700 nm

For more information, visit: www.merckmillipore.com/flwestern

800 nm For product ordering information, see page 115.

Figure 2. Highest sensitivity. Compared to other membranes, Immobilon®-FL membrane enables detection of smaller amounts of protein at all wavelengths, including near-infrared. www.merckmillipore.com 117 Quantify/Detect Protein 118 Product Performance Rapid system takes protein detection to new dimensions SNAP i.d.®2.0Protein Detection System or nitrocellulose blotting membranes. either small volumes for antibodyincubationswith The system’s unique designenablesthe useof (e.g., antigen, antibodyordetection reagents). no additionalreagent consumption capabilities f SNAP and wash steps to 30minutes total. The reducing the blocking, antibodyincubation membrane, enablingthorough washing and vacuum t transport, means ofreagent isthe primary diffusion Unlike conventional Western blotting, where

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Filter Accessories Chemical SNAP i.d.® 2.0 Accessories SNAP SNAP SNAP i.d.® 2.0 Consumables SNAP SNAP SNAP SNAP SNAP Chemical No. SNAP Ordering Information Ordering Description SNAP i.d.® 2.0 Systems SNAP i.d.® 2.0 SNAP SNAP SNAP Chemical Vacuum www.merckmillipore.com For Product Highlight

Antibody recovery and reuse in the SNAP i.d.® 2.0 immunodetection system

Introduction Antibody reuse for Western blotting is a 1. Assemble blot in blot holder/frame 2. Add blocking solution Align positioning common practice. While many antibodies holes on tray lose potency with time or degrade even faster 3. Apply vacuum until completely dry with pins on base 4. Turn vacuum off due to improper storage conditions, there 5. Remove blot holder frame is high potential value of recovering the 6. Wipe any residual liquid at the bottom of the frame primary antibody for possible reuse in some 7. Place antibody collection tray experiments. 8. Add primary antibody and incubate for 10 min or more 9. After incubation, turn vacuum on and wait for 1 min Here, we compare antibody recovery and 10. Turn vacuum off and remove collection tray 11. Turn vacuum on for washing reuse in the standard immunodetection 12. Continue with the SNAP i.d.® protocol protocol with the antibody recovery Figure 1. SNAP i.d.® 2.0 protocol, and reuse in the SNAP i.d.® 2.0 system using including antibody collection. the extended protocol and the original SNAP i.d.® protocol.

Blot processed with freshly Blot processed with Blot processed with antibody Method Processing time diluted antibody collected antibody Processing time used for the third time Protein MCF-7 MCF-7 T47D T47D MCF-7 MCF-7 T47D T47D Standard/ Blocking = 1 h 10 5 2.5 1.210 0.6 5 2.5 10 1.2 5 0.6 2.5 1.2 10 0.6 5 2.5 1.2 0.6 10 5 2.5 1.2 10 0.6 5 2.5 10 1.2 5 0.62.5 1.2 10 0.6 5 2.5 1.2 0.6 Primary Ab =1 h 188 188 188 188 Traditional 98 98 98 98 62 62 Immunodetection Secondary Ab =1h 62 62 49 49 49 49 6 washes (5 min each) 38 38 Method 38 38 = ½ h 28 28 28 28 17 17 Total = 3.5 h 17 17 14 14 14 14 6 6 6 6

MCF-7 MCF-7 T47D T47D MCF-7 MCF-7 T47D T47D MCF-7 T47D SNAP i.d.® Blocking = 20 s 10 5 2.5 1.2 10 0.6 5 2.5 10 1.2 5 0.6 2.5 1.2 0.610 5 2.5 1.2 0.6 10 5 2.5 1.2 10 0.6 5 2.5 10 1.2 5 0.62.5 1.2 10 0.6 5 2.5 1.2 0.6 Blocking = 20 s 10 5 2.5 1.2 0.6 10 5 2.5 1.2 0.6 188 188 188 188 188 Extended Protocol Primary Ab = 1 h 98 98 98 98 Primary Ab = Overnight 98 62 62 62 62 (Extended Secondary Ab =10 min Secondary Ab =10 min 62 49 49 49 49 Quantify/Detect 49 incubation of 8 washes (20 s each) 38 38 38 38 8 washes (20 s each) 38 28 28 28 28 primary Ab only) = 2 min 40 s = 2 min 40 s 28 17 17 Total = 1 h 13 min 17 17 Total = 14 hr 12 min 14 14 14 14 17 14 6 6 6 6 6 MCF-7 MCF-7 T47D T47D MCF-7 MCF-7 T47D T47D

SNAP i.d.® Blocking = 20 s 10 5 2.5 1.2 10 0.6 5 2.5 10 1.2 5 0.62.5 1.2 10 0.6 5 2.5 1.2 0.6 10 5 2.5 1.2 10 0.6 5 2.5 10 1.2 5 0.62.5 1.2 10 0.6 5 2.5 1.2 0.6 188 188 188 188 Original Protocol Primary Ab = 10 min 98 98 98 98 62 62 62 62 Secondary Ab =10 min 49 49 49 49 8 washes (20 s each) 38 38 38 38 = 2 min 40 s 28 28 28 28 17 17 17 17

Total = 23 min 14 14 14 14

6 6 6 6

Figure 2. Two-fold dilution series of breast cancer cell lysates (MCF-7 and T47D, 10 to 0.6 µg total protein) were subjected to SDS-PAGE and transferred to blotting membranes in seven identical blots using three different immunodetection protocols as described above. The seven blots were probed with anti-PP2A, which was either freshly diluted, collected or recovered for a second time.

Conclusion primary antibodies for future reuse. Greater in subsequent immunodetection with no The SNAP i.d.® 2.0 Protein Detection System than 90% of the primary antibody volume reduction in blot quality, even in the extended not only reduces Western blot processing can be recovered after the incubation step protocol (1 h or overnight incubations). time by as much as 80%, but it also conserves by following the recommended protocol. The precious antibodies by providing a rapid antibodies collected can be used successfully For SNAP i.d.® 2.0 system ordering and convenient method for the collection of information, see page 118.

120 www.merckmillipore.com Protein Quantify/Detect 121 S0050 S0100 S0500 WBAVDCH01 WBAVDFL01 WBAVDP001 1 2 x 25 mL WBKL 2 x 50 mL WBKL 2 x 250 mL WBKL 25 mL100 mL WBKDS0025 WBKDS0100 1 1

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multiple tumor types and metastases 1400 • Availability of both human and mouse 1200 angiogenesis/growth factor panels 1000 800

pg/mL 600 400 Bring your research to life: 200 www.merckmillipore.com/milliplex 0 MMP-1 MMP-9 Osteonectin TWEAK sFAs IL-1a sgp130

Identification of tumor-associated biomarkers (cell migration, tumor invasion, angiogenesis and metastasis) in lung tissue lysates derived from tumor-bearing and control (normal) mice. Lung tissue samples harvested 38 days after tumor inoculation and normal lung was harvested from age- matched controls. MILLIPLEX® map Human Cancer, Angiogenesis, Metastasis, Cytokine, Bone, MMP and TIMPs Panels were used to identify biomarker candidates.

www.merckmillipore.com 123 Product Highlight

Reput(Ab)le™ Antibodies We’re validated. We’re guaranteed. We’re published. We create the antibodies most cited by the research community.

Researchers trust our antibodies because we are a thoughtful antibody producer, not a reseller. We’re selective about offering the best antibodies based on the expertise of Chemicon® and Upstate®, internal R&D teams and collaborations with leading institutions. We guarantee our antibodies because of a stringent validation process that produces the highest quality antibodies on the market today.

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A. B.

-H202 / +DNPH +H202 / +DNPH

Oxidative stress detection with OxyBlot™, OxyELISA™, OxyICC™ (shown), IH, and flow cytometry assays. Under physiological and pathophysiological oxidative stress, oxygen free radicals and other reactive species result in carbonyl groups being introduced into protein side chains. Merck Millipore’s oxidative stress detection kits enable simple and sensitive immunodetection of these carbonyl groups, following specific modification with dinitrophenylhydrazine (DNPH). DNPH-modified carbonyls (red) are more abundant in peroxide-treated cells (B), compared to untreated cells (A).

Put the most reputable antibodies to work for you. www.merckmillipore.com/Ab

124 www.merckmillipore.com Trust Merck Millipore membrane filtration devices, chromatography Analytical columns and test kits for the collection, preparation and analysis of your most precious air or fluid samples. In building solutions for Sample your entire analytical research workflow, we’re also minimizing our combined impact on the Preparation environment. Offering both single- use filters, as well as reusable filtration assemblies, we help you create a customized process that’s best for your lab. You’ll get better research results faster, at a lower cost to you and the planet.

Collect Prepare Test

Collect samples from air, soil, water, fuel and industrial processes using our membrane filters, filter holders and solvent dispensers. Many of our page 147 page 207 sample collection devices feature broad chemical compatibility, low extractables and low analyte binding, making them ideal for sensitive analyses. Filter Holder Selection Guide Glass Stainless Steel (SS) Plastic Vacuum Vacuum Pressure Vacuum Pressure

13 mm Epifluorescence Swinny Filter Swinnex® Holder Filter Holder Holder (page 190) (page131) (page 191)

Analytical Filter Holder (page 130)

25 mm Microanalysis Filter Analytical Filter Holder High Pressure Filter 1225 Sampling Manifold Swinnex® Holder Holder (page 129) (page 130) Holder (page 193) (page 140) (page 190)

Solvent Filtering Dispenser (page 141)

Microsyringe Filter Holder (page 191) Analytical Filterjet™ Solvent Dispenser (page 142) Collect

47 mm All-Glass Analytical SS Pressure Filter Sterifil® Swinnex® Holder Filter Holders Filter Holder Holder (page 196) System (page 190) (page 127) (page 130) (page 131)

Classic Glass Hydrosol™ Filter Holder High Pressure Filter Sterifil® 500 Filter Holder In-Line Filter Holder Filter Holder (page 133) Holder (page 193) (page 131) (page 192) (page 128)

MilliSolve™ System (page 197)

90 mm All-Glass Filter Standing SS Holder Filter Holder (page 127) (page 195)

142 mm Standing SS Filter Holder (page 195)

126 www.merckmillipore.com Analytical Collect 127 Qty/Pk Catalogue No. 11 XX2004708 25 XX1004703 XX2004703 11 XX1009000 1 XX1009002 XX1009003 10 XX1009010

with

chemical simplifies

liquid,

cap

broad

For more information visit: www.merckmillipore.com/LabHardware visit: For information more Note: completed replacement part information available online. available part information replacement completed Note: Description support Base and Cap, stainless steel Vacuum stainlessSupport steel Screen, aluminum anodized Clamp, Spring 1 PTFE 47 mm Gasket, XX1504732 PartsReplacement (90 mm Holder) 1 L, 90 mm Glass Funnel, stainlessSupport steel Screen, aluminum anodized Clamp, Spring support Base and Cap, stainless steel Vacuum PTFE 90 mm Gasket, 1 XX1009004 contact flask

has

parts

glass

construction 90 mm Holder stainless steel PTFE-coated N/A 90 40 35 (funnel and base) 11.5 Funnel: connection

patibility ansfer of filtrate ansfer round-glass sealing surfaces round-glass com g tr • Vacuum Applications Particle Solvent Contamination Analysis, HPLC Filtration, Filtration General and Clarification Features& Benefits • Borosilicate • All-glass Qty/Pk Catalogue No. 11 XX1504705 XX1604705 13 XX7100004 XX6200006P 11 XX1504732 XX1004707 11 XX1004704 XX1504702 11 XX1504700 XX1009020 47 mm Holder aluminum cap; anodized funnel, base and tubulated glass Borosilicate clamp spring glass* Fritted glass Borosilicate 47 9.6 1 300 mL; flask: Funnel: vacuum to cap sidearm tubulated 6 mm (1/4 in.) O.D. Funnel: 1 L L 43 14; funnel: 7.6 Flask: 2 Replacement PartsReplacement (47 mm Holder) 300 mL, 47 mm Glass Funnel, 2 L Ground Joint Flask 2 L Ground Conical Bottom with Joint Flask 5 L Ground 3/16 in. I.D. x 140 cm, silicone Tubing, blunt end, stainless Forceps, Filter 1 Screen, Steel Stainless Glass Base & Cap with XX1604706 47 mm seal glass 47 mm, ground 1L Funnel, glass Base and Cap, fritted Vacuum Accessories (47 mm Holder Only) Accessories 1 L Ground Joint Flask 1 L Ground Accessories (47 mm and 90 mm Holders) Accessories Description base, Funnel, with Holder Assembly All-Glass Filter and flask, 47 mm cap, clamp, base, Funnel, with Holder Assembly All-Glass Filter 90 mm cap, clamp, * Optional glass base with stainless steel screen available. screen stainless steel base with glass * Optional Filter SupportFilter Flask mm Diameter, Filter cm Area, Filtration mL Capacity, Volume Fitting Outlet Dimensions Height, cm cm Diameter, Specifications Materials Base, and Cap Funnel, The All-Glass Filter Holder was designed Holder was Filter The All-Glass and organic aqueous of filtration the for particulate for liquids or corrosive contamination analysis. can The holder preparation. solvent HPLC also be used for into is integrated The vacuum connection the cap, above holder base and flask filter the you can this design, With exit level. filtrate into filtrate drawing unintentionally avoid simple also allows vacuum tubing. This design by solvents) (i.e., HPLC of filtrate transfer flask. receiver the from pouring Ordering Information Ordering www.merckmillipore.com For filtration of corrosive liquids and solvents and liquids For of corrosive filtration All-Glass Filter Holder (47 mm, 90 mm) 90 (47 mm, Holder Filter All-Glass Classic Glass Filter Holders For 47 mm disc filters The Classic Glass Filter Holder is an essential • Three membrane support material options tool for every research lab. The holder’s to suit a variety of applications multipurpose design is appropriate for a • UV sterilize or autoclave with filter in place variety of applications and is available in three for bacteriological analysis different support materials for compatibility with your specific application needs. Applications General Clarification, Bacteriological Analysis, Features & Benefits Particulate Contamination Analysis of Oils • Designed for easy loading and removal of and Hydraulic Fluids, Exfoliative Cytology membranes

Specifications Materials of Construction Borosilicate glass funnel and base; anodized aluminum spring clamp; silicone stopper Glass Support (cat. no. XX1004700) Coarse-frit glass filter support PTFE Support (cat. no. XX1004720) PTFE-faced funnel and base Stainless Screen Support Stainless steel screen filter support (cat. no. XX1004730) Filter Diameter, mm 47 Filtration Area, cm² 9.6 Funnel Capacity, mL 300; accessory 1 L is available Prefilter Diameter, mm 35 (thick depth prefilter) or 47 (membrane prefilter) Outlet Fitting No. 8 perforated silicone stopper mounts in standard 1 L and 4 L

Analytical filtering flasks Dimensions Height, cm 22.9 Diameter, cm 7.6 Sterilization Method Collect Glass Supported and Stainless UV sterilize or autoclave without filter in place Screen Supported Holders PTFE Supported Holder Autoclave with filter in place

Ordering Information Description Qty/Pk Catalogue No. Description Qty/Pk Catalogue No. Glass Filter Holder Assembly, with funnel, fritted 1 XX1004700 Glass Base with Screen, 47 mm 1 XX1004732 base, stopper, clamp, 47 mm Glass Base without Screen, 47 mm 1 XX1004733 Glass Filter Holder Assembly, PTFE-coated, with 1 XX1004720 Support Screen, 47 mm, stainless steel 1 XX2004708 funnel, base, stopper clamp, 47 mm Gasket, PTFE 25 XX2004703 Glass Filter Holder with stainless steel screen, 47 mm 1 XX1004730 No. 8 Perforated Stopper, silicone 5 XX1004708 Replacement Parts: Glass Filter Holder Note: complete replacement parts information available online. Glass Funnel, 300 mL, borosilcate 1 XX1004704 Accessories Spring Clamp, 47 mm, aluminum 1 XX1004703 Vacuum Filtering Flask, 1 L 1 XX1004705 Base for 47 mm glass/filter holder 1 XX1004702 Vaccum Filtering Flask, 4 L 1 XX1004744 No. 8 Perforated Stopper, silicone 5 XX1004708 1 L Funnel, 47 mm, ground glass seal 1 XX1004707 Replacement Parts: PTFE-faced Glass Filter Holder Tubing, 3/16 in. I.D. x 140 cm, silicone 1 XX7100004 Funnel, PTFE-faced, 300 mL 1 XX1004724 High Output Pump, 115 V/60 Hz 1 WP6211560 Spring Clamp, 47 mm, aluminum 1 XX1004703 High Output Pump, 220 V/50 Hz 1 WP6222050 Glass Base, PTFE-faced, 47 mm 1 XX1004722 High Output Pump, 100 V/50–60 Hz 1 WP6210060 No. 8 Perforated Stopper, silicone 5 XX1004708 Replacement Parts: Stainless Screen Glass Filter Holder Glass Funnel, 300 mL, borosilcate 1 XX1004704 Spring Clamp, 47 mm, aluminum 1 XX1004703 For more information visit: www.merckmillipore.com/LabHardware

128 www.merckmillipore.com Analytical Collect 129 Qty/Pk Catalogue No. 11 XX1002500 XX1002530 11 XX1002514 1 XX1002503 1 XX1002502 5 XX1002532 10 XX1002508 1 XX1002535 XX5002501 Borosilicate glass funnel and base; fritted glass glass funnel and base; fritted glass Borosilicate clamp; spring aluminum support; anodized filter silicone stopper funnel and base; removable glass Borosilicate support; anodized filter screen stainless steel silicone stopper clamp; aluminum spring ~25 2.5 15 in mounts stopper silicone 5 perforated No. flask* 125 mL filtering standard 15.2 2.5 Applications use with for Contamination Analysis (not or flammable liquids) prefilters thick

sample

glass

indicate

fritted

clearly

available

graduations

support

olume tainless steel v s Replacement PartsReplacement glass borosilcate Funnel,15 mL, Microanalysis Filter Holder, 25 mm, fritted glass support glass 25 mm, fritted Holder, Filter Microanalysis Ordering Information Ordering Description support 25 mm, stainless steel Holder, Filter Microanalysis 25 mm, aluminum Clamp, Spring 25 mm Stopper, Glass Base with Fritted Screen Steel and Stainless Base Stopper silicone Stopper, 5 Perforated No. PTFE coated Gaskets, 25 mm, stainlessSupport steel Screen, *Filter holders can be used on 1 L flasks using a No. 8 stopper (cat. no. XX2004718). holders*Filter can be used on 1 L flasksstopper No. 8 using a Specifications Materials glass mm, fritted 25 Holder, Filter (cat. no. XX1002500) Filter Holder, 25 mm, stainless steel steel mm, stainless 25 Holder, Filter (cat. no. XX1002530) mm Diameter, Filter cm² Area, Filtration mL Capacity, Funnel Fitting Outlet Dimensions Height, cm cm Diameter, www.merckmillipore.com For more information visit: www.merckmillipore.com/LabHardware visit: For information more • Filter Features & Benefits • Volume For small-volume contamination analysis or biological For small-volume is holder filter microanalysis The glass of small- filtration vacuum designed for particulateor for samples liquid volume biological contamination analysis. Glass Microanalysis Filter Holders Filter Microanalysis Glass Analytical Stainless Steel Filter Holders for 13, 25, and 47 mm Disc Filters For contamination analysis The analytical stainless steel filter holders Applications are designed for the vacuum filtration of Bacteriological or Particulate Contamination samples for bacteriological or particulate Analysis contamination analysis.

Features & Benefits • Available in three diameters with four available funnel sizes to accommodate samples up to 250 mL • Holder with 250 mL funnel is equipped with a funnel lid, which can be vented using accessory filter unit • Can be autoclaved or flame-sterilized

Specifications 40 mL Holder 50 mL Holder 100 mL Holder 250 mL Holder Materials Assembly Stainless steel funnel and Stainless steel funnel and support Stainless steel funnel, Stainless steel funnel, support screen support screen screen; borosilicate glass base; support screen and base; and base; stainless steel funnel cover; anodized aluminum spring clamp; anodized aluminum spring anodized aluminum spring clamp; silicone stopper clamp; silicone stopper silicone stopper Gasket PTFE PTFE No gasket needed No gasket needed Filter Diameter, mm 13 25 47 47 Analytical Filtration Area, cm² 0.7 2.8 9 9 Dimensions Height, cm 8.9 12.6 15.6 19.4 Diameter, cm 3.8 5.1 6.2 7.6 Collect Outlet Male Luer-Slip™ No. 8 perforated silicone stopper mounts in standard 1 L filtering flask

Ordering Information

Description Qty/Pk Catalogue No. Description Qty/Pk Catalogue No. 40 mL Filter Holder, 13 mm, stainless steel 1 XX3001240 Replacement Parts for 100 and 250 mL Holders 50 mL Filter Holder, 25 mm, stainless steel 1 XX1002540 Stainless Steel Funnel, 100 mL 1 XF2004755 100 mL Filter Holder, 47 mm, stainless steel 1 XF2004710 Stainless Steel Funnel, 250 mL 1 XF2004756 250 mL Filter Holder, 47 mm, stainless steel 1 XF2004725 Spring Clamp, 47 mm, aluminum 1 XX1004703 Replacement Parts for 40 mL Holder No. 8 Perforated Stopper, silicone 5 XX1004708 Replacement Parts Kit for 40 mL Holder 1 XX30012RK* Stainless Steel Support Frit 1 XF2004758 *Kit includes (4) PTFE O-rings, (4) PTFE gaskets, (4) stainless steel support screens Accessories Replacement Parts for 50 mL Holder Millex®-FG Filter Unit, 0.20 µm, hydrophobic PTFE, 50 SLFG02550 Spring Clamp, 25 mm, aluminum 1 XX1002503 25 mm Base Assembly for 25 mm Filter Holder 1 XX1002542** Vacuum Filtering Flask, 1 L 1 XX1004705 No. 8 Stopper, 9.5 mm (3/8 in.) hole, silicone 5 XX2004718 Vaccum Filtering Flask, 4 L 1 XX1004744 Support Screen, 25 mm, stainless steel 1 XX5002501 Tubing, 3/16 in. I.D. x 140 cm, silicone 1 XX7100004 Gaskets, PTFE coated 10 XX1002535 **Base with Support Screen, Gasket, and Stopper

For more information visit: www.merckmillipore.com/LabHardware

130 www.merckmillipore.com Analytical Collect 131 Qty/Pk Catalogue No. 1 XF3001200 Applications Filtration,General Field-based Filtration Sterile hand pump), optional (with Counting Monitoring, Filtration, Bioburden Filtration Processes Microorganisms, be Retained to Membrane Requiring Applications Epifluorescence Analysis by Bacteriological Microscopy

filtrate

on-site

and Stainless steel funnel and base; anodized aluminum spring clamp; clamp; aluminum spring anodized funnel and base; steel Stainless silicone stopper 13 0.7 250 flask 1 L filtering mounts in standard silicone stopper 8 perforated No.

for

membrane

with

allows samples

pump

protects

hand autoclavable

unit is

or sterile applications with less waste applications with or sterile

ation om environmental contamination om environmental filtr fr place f Description stainless 13 mm, steel Holder, Filter Epifluorescence Ordering Information Ordering Materials Specifications mm Diameter, Filter cm² Area, Filtration mL Capacity, Funnel Fitting Outlet www.merckmillipore.com • Optional • Device Features & Benefits • Closed Reusable, autoclavable device with removable membrane removable with device autoclavable Reusable, was designed as system aseptic The Sterifil® applications and filtration general for a tool for particulate or biological of samples filtration holder and contamination analysis. Sterifil® receiver (without separately available funnel are 1 L filter standard to use with and cover) flask flasks or multiple place manifolds. Sterifil® Aseptic System and Holder System Aseptic Sterifil® For more information visit: www.merckmillipore.com/LabHardware visit: For information more For bacteriological analysis by epifluorescence by analysis For bacteriological vacuum quantitative accurate, Designed for bacteriological for samples of filtration the microscopy, epifluorescence analysis by funnel prevents stainless polished steel highly surface. to adhering from cells bacterial Epifluorescence Stainless Steel Filter Holder Filter Steel Stainless Epifluorescence Specifications Materials Polysulfone funnel, funnel cover, receiver flask and cover; polypropylene holder base and filter support screen; silicone stopper Sterifil® Aseptic System Sterifil® 500 Holder Dimensions Height, cm 20.3 14.5 Diameter, cm 10.8 8.5 Filter Diameter, mm 47 47 Filtration Area, cm² 13.8 15.2 Funnel Capacity, mL 250 500 Prefilter Diameter, mm 42 (thick depth prefilter) or 47 (membrane prefilter) Outlet Fitting Holder outlet stopper fits standard 1 L filtering flask Receiver Flask Fitting Receiver flask ports accept 6 mm (1/4″) I.D. tubing or male Luer-Slip™ connection for vacuum, drain, or vent. Cover Inlet and Vent Ports Female Luer-Slip™ connection

Ordering Information Description Qty/Pk Catalogue No. Sterifil® Aseptic System, 47 mm 1 XX1104700 Sterifil® Holder, 250 mL (excludes receiver flask) 1 XX1104710 Sterifil® Holder, 500 mL 1 XX11J4750 Replacement Parts Analytical Sterifil® Funnel Cover 1 XX1104703 Caps, gum rubber 100 XX1104711 Swinnex® Filter Holder, 13 mm 10 SX0001300 Glass Fiber Filter with Binder, AP25, 13 mm 100 AP2501300 Collect Sterifil® Funnel, 250 mL, 47 mm, Polysulfone 1 XX1104704 Filter Holder Base and Support Screen 1 XX1104702 Support Screen, 47 mm, polypropylene 1 XX1104715 O-ring (5-329) for Swinnex® 47 mm filter holder, silicone 10 XX1104707 No. 8 Stopper, 9.5 mm (3/8 in.) hole, silicone 5 XX2004718 Cover for Sterifil® Receiver Flask 1 XX1104706 Sterifil® Receiver Flask, 250 mL 1 XX1104705 Note: complete replacement parts information available online. Accessories Tubing, 3/16 in. I.D. x 140 cm, silicone 1 XX7100004 Hand Vacuum Pump, polypropylene 1 XKEM00107 EZ-Fit® Manifold, 3-place, stainless steel 1 EZFITMIC03 High Output Pump, 115 V/60 Hz 1 WP6211560 High Output Pump, 220 V/50 Hz 1 WP6222050 High Output Pump, 100 V/50–60 Hz 1 WP6210060

For more information visit: www.merckmillipore.com/LabHardware

132 www.merckmillipore.com Analytical Collect 133 1 XX2004720 11 XX2004704 1 XX2004708 25 XX2004702 5 XX2004703 1 XX2004718 XX20047RK* 11 XX1004705 1 XX1004744 3 XX7100004 1 XX6200006P 1 XX2604735 1 XX2504735 1 XX2504700 1 WP6211560 1 WP6222050 WP6210060 Qty/Pk Catalogue No. Applications Filtration of Flammable Liquids Vacuum

other

Stainless steel funnel, base and filter support screen; anodized anodized support filter funnel, base and screen; steel Stainless silicone stopper lockwheels; nylon ring; aluminum locking 47 9.6 650 prefilter) or 47 (membrane prefilter) depth 35 (thick flask 1 L filtering in standard mounts stopper silicone 8 perforated No. 22.9 11.4 Unlike

liquids.

flammable

er holders, the Hydrosol™ filter holder filter Hydrosol™ er holders, the Vacuum Filtering Flask, 1 L Filtering Vacuum *Replacement parts kit includes (1) 47 mm aluminum locking ring, (1) hex key, (1) nylon locking wheel with wrench, wrench, wheel with locking (1) nylon (1) hex key, parts ring, kit includes (1) 47 mm aluminum locking *Replacement gaskets ring (5) PTFE locking gaskets, (5) PTFE screen Accessories Support Screen, 47 mm, stainless 47 mm, Support steel Screen, and stopper gasket Screen, Base with PTFE Gasket, 9.5 mm (3/8 in.) hole, silicone Stopper, No. 8 Holder Filter Parts Hydrosol™ for Kit Replacement Flask, 4 L Filtering Vaccum 3/16 in. I.D. x 140 cm, silicone Tubing, blunt end, stainless Forceps, Filter steel 47 mm, PVC 3-place Manifold, 47 mm, stainless steel 3-place Manifold, 47 mm, stainless steel 6-place Manifold, 115 V/60 Hz High Output Pump, 220 V/50 Hz High Output Pump, 100 V/50–60 Hz High Output Pump, Funnel and Locking Ring Assembly, stainless steel Ring Assembly, and Locking Funnel Hydrosol™ Filter Holder, 47 mm, stainless steel Holder, Filter Hydrosol™ PartsReplacement Ordering Information Ordering Description Specifications Materials Filter Diameter, mm Diameter, Filter cm² Area, Filtration mL Capacity, Funnel mm Diameter, Prefilter Fitting Outlet Dimensions Height, cm cm Diameter, www.merckmillipore.com For more information visit: www.merckmillipore.com/LabHardware visit: For information more filt For liquids of flammable analysis the for is designed holder filter The Hydrosol™ filtration sparking prevent unit to includes a grounding be a safety can which static discharge, from hazard. Hydrosol™ Stainless Steel Filter Holder Filter Steel Stainless Hydrosol™ Aerosol Filter Holders For environmental monitoring These aerosol filter holders are designed for Applications monitoring airborne particulates from the Particulate Contamination Monitoring environment and/or closed systems. Choose in Gases the holder design most appropriate for your method. Aerosol filter holders are available in two formats: open-type for open atmosphere sampling and a standard design with a closed connection and inlet dispersion chamber.

Specifications Materials Stainless steel bodies, filter support screens; anodized aluminum locking rings; nylon lockwheels Dimensions Filter Diameter, mm 47 Filtration Area, cm² 9.6 Length, cm of Std. Holder 17.8 Length, cm of Open Holder 10.2 Diameter, cm 6.9 Connections Inlet – Std. Holder Hose connector (11 mm O.D.) Outlet Closed system connection, with inlet dispersion chamber

Analytical and 11mm O.D. inlet/outlet connections, threaded for flow-limiting orifices

Collect Ordering Information Description Qty/Pk Catalogue No. Aerosol Standard Filter Holder, 47 mm, stainless steel 1 XX5004700 Aerosol Open Filter Holder, 47 mm, stainless steel 1 XX5004710 Aerosol Clean Room Filter Holder, 47 mm, stainless steel 1 XX5004740

For more information visit: www.merckmillipore.com/ParticleMonitoring

134 www.merckmillipore.com Analytical Collect 135

will

and polymer

X-ray

PVC

humidity stress

sensitive 3 XX6200006P

thermal

for to

medical-grade

Plain 50 AG4502550 resistant non-hygroscopic,

silver background is

ressive chemicals ressive fraction analysis fraction t impact your gravimetric analysis gravimetric your t impact agg dif no WhiteWhite PlainWhite Plain 100 Plain 100 PVC502500 100 PVC503700 PVC504700 • Highly Applications Lead Sulfide, Asbestos, Air Monitoring: Crystalline Silica and Amorphous • Low Features & Benefits • Pure • PVC Applications of Particulate Contaminants Air Monitoring Quartz and Including Silica, Metals, Features& Benefits • High-quality, 25 25 37 47 Pore Size (µm) Size Pore (mm) Diameter Surface Qty/Pk Catalogue No. Pore Size (µm) Size Pore (mm) Diameter Color Surface Qty/Pk Catalogue No. Filter Forceps, blunt end, stainless Forceps, Filter steel Description MembraneSilver Accessory 0.45 Ordering Information Ordering Description MembranePVC 5 Ordering Information Ordering For more information visit: www.merckmillipore/filterdiscs visit: For information more Silver membrane filters are suitable for a suitable are filters membrane Silver commonly variety of applications and are and analysis of air monitoring used for from particulate contaminants. Methods NIOSH specify like organizations standards and for collection filters membrane silver of particles as silica, carbon such detection oven and coke black, coal tar products, emissions. www.merckmillipore.com For more information visit: www.merckmillipore/filterdiscs visit: For information more Polyvinylidene chloride (PVC) membranes membranes (PVC) chloride Polyvinylidene by ASTM,NIOSH, and OSHA in specified are silica, analyze to applications air monitoring particulates and quartz metals, black, carbon others. Pure silver filters for particulate filters monitoring silver Pure Silver Membrane Filters Membrane Silver Pure, medical-grade PVC for sensitive air monitoring air sensitive for PVC medical-grade Pure, PVC Membrane Filters Membrane PVC Application Spotlight

Radioactive Alpha Particle Monitoring

In order to reap the benefits of nuclear energy The Fluoropore™ filter for alpha responsibly, users and regulatory organizations particle monitoring provides: must monitor the degree to which radioactive • Improved detection accuracy. Unlike byproducts of human activity enter the fibrous filter media that trap particles environment. Human activities that generate in their matrix, Fluoropore™ membranes radioactive particles include scientific collect particles on their surface. This experiments involving radiation, medical surface collection keeps particles closer therapies, nuclear-powered submarines, x-ray to the detector, providing better detection procedures, nuclear power plants and smoke efficiencies and improved resolution. detectors. Although there are many naturally occurring radioisotopes in water, air, soil, • Choice of pore sizes. Fluoropore™ and organisms, the challenge in radiation membranes are available in 3 and 5 μm monitoring is to detect the “nonnatural” pore sizes. The smaller pore size provides radiation signal above the naturally occurring high resolution. The larger pore size background radiation levels. has very low pressure drop and greater throughput capacity, which minimizes the To support efficient, convenient radiation number of filter change-outs. monitoring, Merck Millipore has developed • Contr asting backing material. The 5 μm a special Fluoropore™ PTFE membrane filter Fluoropore™ membrane is bonded to a

Analytical designed for alpha particle collection in gridded backing made from high density single-use and continuous air monitors. polyethylene fiber. The contrast between Fluoropore™ membrane is approved for use by the top and bottom of the filter prevents the French Institute for Radiological Protection incorrect installation in sampling devices.

Collect and Nuclear Safety in Saclay, France as well as The backing also reduces the electrostatic national labs in North America and Asia. charge of the filters and makes them easier to handle.

A. B.

7 700

6 600

5 500

4 400

3 300 FWHM (kEv)

2 200 Pressure Drop (in.Hg) Drop Pressure 1 100

0 0 5 µm 3 µm Glass MCE Reinforced MCE 5 µm 3 µm Reinforced Glass MCE Fluoropore™ PTFE Fiber 5 µm MCE 3 µm Fluoropore™ PTFE MCE Fiber 5 µm Membrane (Type A) Membrane (Type A)

Lower pressure drop, lower FWHM* measurements. Merck Millipore’s Fluoropore™ PTFE membranes exhibit lower pressure drop (A) and collect particles on the surface , providing better detection efficiencies and lower FWHM measurements (B). Room air was collected at 1.7 m3/h (1 standard cubic foot/min). *FWHM, a measure of spectral quality/resolution, is defined as the full width at half of the maximum height of the P0-218 peak obtained during air sampling of room air.

For ordering information see page 178.

136 www.merckmillipore.com Analytical Collect 137 505050 M000025A0 M000037A0 M00003700

White White WhiteWhite Plain PlainWhite Plain 50White Plain 50White Plain 50White 50Black Plain MAWP025A0 50White Plain MAWP037A0 White Gridded MHWP037A0 100White Gridded MAWP037AM 100White Plain 100 MAWP025AC Gridded 100 SMWP03700 Plain 100 AAWP03700 Plain 100 AAWG03700 AABG03700 100 100 HAWP03700 HAWG03700 GSWP03700 LSWP03700 filter

methods

membranes

the gridded

place

Background

or in

seals

standard

black

filters

Average

25 37 37 25 37 37 37 25 37 37 37 37 37 37 37 37 NIOSH support

with matched-weight white,

with

with with

anes in two pore sizes pore anes in two cellulose marked

tween monitor sections to distribute distribute sections to monitor tween thin

o save time during gravimetric analysis gravimetric time during o save sample flow evenly over the filter surfacethe filter over evenly flow sample Count (ABC) values Count (ABC) Pre-assembled membr t be 0.8 0.8 0.45 0.8 0.8 0.8 0.8 0.8 0.45 0.45 0.22 Pore Size (µm) Size Pore (mm) Diameter Color Filter Surface Filter Qty/Pk Catalogue No. • Boxes • Available • A Applications Montitoring, Environmental Air Monitoring, Asbestos Contamination Monitoring, Aerosol Monitoring • Features& Benefits • Compliant • Available MF-Millipore™ MCE membrane / cellulose pad MCE membrane MF-Millipore™ Polystyrene polypropylene Carbon-filled fit Female Luer-Slip™ fit Female Luer-Slip™ Non-sterile

MAWP025AC)

No.

(Cat.

monitors

Use

Empty Monitor Cassette without Ring without Cassette Monitor Empty Empty Monitor Housings Empty Monitor A Type Cassette, Monitor Empty Monitor Refills Monitor Pad Absorbent Thin with MCE Refills MF-Millipore™ 5.0 Type A, Aerosol Monitor A, Aerosol Type Ordering Information Ordering Description filters membrane with Monitors, complete Monitor Aerosol A, Matched-Weight Type Monitor B, Aerosol Type Pad Absorbent Thin with Mitex™ PTFE Refills 5.0 Materials and SupportFilter Pad Specifications Case A Monitor Type Case B Monitor Type Connections Inlet Outlet Sterility or airborne asbestos monitoring in accordance monitoring asbestos or airborne For more information visit: www.merckmillipore.com/ParticleMonitoring visit: For information more f NIOSH specifications. with Type B is a three-piece carbon-filled B is a three-piece Type with monitor conductive polypropylene 25 mm and 37 mm diameters, with female with 25 mm and 37 mm diameters, A monitors vacuum connection. Type Luer-Slip™ membrane. without also available are hose vacuum Barbed 50 mm extension cowl. tubing need for the connection eliminates adapter. Two types of three-piece aerosol contaminationtypes aerosol three-piece of Two A) (Type PS monitor offered: the are monitors A is a clear, B). Type (Type PP monitor and the ring. a 16 mm center with polystyrene monitor in place filter design holds the The three-piece open for section is removed top when the in available are Monitors sampling. aerosol www.merckmillipore.com Aerosol Contamination Monitors Aerosol With 25 mm and 37 mm filters 25 mm and With Aerosol Analysis Accessories For environmental monitoring The Aerosol Adapter adapts your vacuum Features & Benefits source to an aerosol analysis monitor, • Modulate flow rate to meet specified flow Swinnex® 47 mm filter holder or other rates without the need for additional flow products with female Luer inlets. Attach a regulators or valves flow-limiting orifice for quantitative aerosol • Flow-limiting orifice ratings are accurate sampling. The adapter’s vacuum-hose end to within approximately 15%; for greater fits the hole of a No. 8 silicone stopper, precision, calibrate orifice with a flowmeter (cat. no. XX1004708) and the opposite male at actual working vacuum level Luer-Slip™ end fits Swinnex® filter holders or monitor outlet connections. Applications Air Monitoring, Environmental Monitoring, Flow-limiting orifices create quantitative Aerosol Contamination Monitoring airflow through an MF-Millipore™ filter for airborne contamination analysis. Thread the orifice into the outlet of an aerosol analysis holder or aerosol adapter for a constant flow rate.

Specifications Orifice Flow Rate (L/min) mm Hg in Hg PSIG 1 300 12 6 3 300 12 6 Analytical 4.9 400 16 8 10 500 20 10 14 550 22 11 Collect

Ordering Information Description Qty/Pk Catalogue No. Flow-limiting Orifices, set of 5 1 XX5000000 Flow-limiting Orifice, 2 L/min 1 XX5000020 Flow-limiting Orifice, 1 L/min 1 XX5000002 Aerosol Adapter, Luer-Slip™ to 1/4 in.– 3/8 in. I.D. hose, stainless steel 1 XX6200004

For more information visit: www.merckmillipore.com/ParticleMonitoring

138 www.merckmillipore.com Analytical Collect 139 50 MAWP037PM 50 MAWP037P0 50 M000037P0 Applications Contamination Monitoring Liquid

liquid

White Plain White Plain high with

with

membranes multiple

place

in during

monitors combine

compatible

filters to

your 100100100 AP1001300 100 AP1002500 100 AP1003700 50 AP1004700 AP30034P0 AP3212450 damage are

you with of 0.8 Pore Size (µm) Size Pore 0.8 Color Filter Surface Filter Qty/Pk Catalogue No.

matched-weight

life

with

enable housings

the

ane refills, both standard membane standard both ane refills,

membrane

essure or fast flow conditions or fast flow essure ydrocarbon fuels ydrocarbon tages of filtration into a single process process a single into of filtration tages o save time during gravimetric analysis gravimetric time during o save Diameter (mm)Diameter Qty/Pk Catalogue No. 13 25 37 47 34 124 pr s time saving step, and matched-weight pairs and matched-weight h t membr • Spacers Applications Monitoring, Environmental Air Monitoring, Contamination Monitoring Aerosol • Tenite® • Available • Extend Features & Benefits • Avoid retains all contaminants. The difference in retains weight weights, after drying,the filter equals of contaminants in sample. Features & Benefits • Pre-assembled

the

with

Use

surface.

filter

the

pling Kit (cat. no. XX6403730). over

Matched-Weight Fluid Contamination Analysis Monitor Matched-Weight Thick Absorbent Pad Absorbent Thick Mesh Spacer Woven Absorbent Pad Absorbent Ordering Information Ordering Description Accessories empty Fluid Contamination Monitor, Description Fluid Contamination Analysis Monitor Ordering Information Ordering For more information visit: www.merckmillipore/filterdiscs visit: For information more www.merckmillipore.com Cellulose pads and Dacron® mesh spacers Cellulose pads and Dacron® reinforce used to Cellulose support pads are for contamination analysis. in monitors filters medium, they growth with When saturated microorganisms culturing can also be used for are mesh spacers Woven on membranes. filtration serial during filters placed between from filter screen downstream the prevent to They pores. filter upstream “blinding” the throughput. and rate flow increase Filter SupportFilter Materials For more information visit: www.merckmillipore.com/ParticleMonitoring visit: For information more 34 mm diameter, between the monitor sections monitor the between 34 mm diameter, flow the sample distribute to filter seals the evenly Fluid Sam the eliminate Monitors Matched-Weight gravimetric in filters test pre-weigh need to filters contains two monitor analysis. Each 0.1 mg. Sample within to in weight matched filter but upper (test) both, passes through For contamination analysis are Fluid Contamination Analysis Monitors holders made filter disposable transparent, with and pre-assembled plastic Tenite® from pad, cellulose thick A filters. MF-Millipore™ Fluid ContaminationFluid Monitors 1225 Sampling Manifold For multi-sample vacuum filtration Simultaneously vacuum filter 12 samples Applications for rapid analysis of retentate or filtrate. General Filtration of 15 to 50 mL Samples, Numbered positions on the filter plate assure Preparation for Scintillation Counting accurate sample tracking. Sealing stoppers maintain vacuum on unused sample cups. Accessory extension barrels increase sample volumes from 15 to 50 mL.

Specifications Materials Glass-filled polypropylene plates, chamber, handwheel nut, valve; polypropylene test tube rack, support screens; HDPE drain plug; 316 stainless steel bolt; brass handwheel insert Filter Diameter, mm 24 or 25 Filtration Area, cm² 2.7 per filter Cup Volume, mL 15; 50 with extension barrel Fittings 1/4 in. NPTF for vacuum valve and drain plug Dimensions Height, cm 17.7 Diameter, cm 20.5 Shipping Weight, kg (lb) 2.9 (6.4) Sterilization Method Can be autoclaved a minimum of 20 times (screens and O-rings

Analytical not autoclavable) Chemical Compatibility Acid- and base-resistant (includes TCA). Solvent-resistant at temperatures <80 °C. Not for strong oxidizing acids. Collect

Ordering Information

Description Qty/Pk Catalogue No. 1225 Sampling Manifold 1 XX2702550 Replacement Parts Hand Knob 1 YY2214257 Top Plate 1 5162 Filter O-ring, silicone 30 XX2702509 Support Plate 1 5163 Support Screen, 25 mm, polypropylene 30 XX2702510 Ball Valve, glass-filled polypropylene 1 XX11000PP Tubing, 1/4 in. x 23 in., latex 1 XX2504755 Note: Complete replacement parts information available online. Accessories Chamber O-rings and Plug Set 1 XX2702552 Sample Cup Extension Barrel 12 XX2702555 Filter Forceps, blunt end, stainless steel 3 XX6200006P Tubing, 3/16 in. I.D. x 140 cm, silicone 1 XX7100004

140 www.merckmillipore.com Analytical Collect 141

need

the

eliminates

Qty/Pk Catalogue No. 11 XX6602500 101 XX6602501 XX3002502 XX3002510 solvent

pump

hand

or external pump or external f • Holds Features& Benefits • Integrated Applications Contamination to Filtration Prior Solvent Parts Rinsing of Machined Analysis, Solvent and Collection Containers

Use

Borosilicate glass flask; neoprene bulb and stopper; stainless steel filter filter stainless steel stopper; bulb and neoprene flask; glass Borosilicate nozzle and dispensing elbow glass and tubing; PTFE gaskets holder; 25 3.9 1.2* 1 31.8 13.3 volumes

tube.

small

delivery

dispense

with

and

fitted

vent by squeeze-bottle action. This hand- action. squeeze-bottle by vent ect filtered solvent against surfaces. against solvent ect filtered Filter Holder Gasket, PTFE Holder Gasket, Filter stainless Holder SupportFilter steel Screen, Flask and Neoprene Stopper and Neoprene Flask Description 25 mm Dispenser, Filtering Solvent PartsReplacement Ordering Information Ordering *25 type RAWP filter discs are included with dispenser. included with discs are filter *25 type RAWP Filter Diameter, mm Diameter, Filter cm² Area, Filtration µm Size, Pore L Capacity, Flask Dimensions Height, cm cm Diameter, Specifications Materials For more information visit: www.merckmillipore.com/ParticleMonitoring visit: For information more Point-of-use, hand-operated solvent filtration solvent hand-operated Point-of-use, Solvent Filtering Dispenser Filtering Solvent www.merckmillipore.com pressure operated dispenser features a filter a filter features dispenser operated pressure holder dir Ultraclean sol Filterjet™ Solvent Dispenser Point-of-use solvent filtration Apply a concentrated jet spray of ultracleaned Applications solvent or rinse solution to surfaces that Solvent Filtration Prior to Contamination require washing. Just connect the Filterjet™ Analysis, Solvent Rinsing of Machined Parts dispenser to a dispensing pressure vessel and Collection Containers to ensure that the solvent or other liquid is filtered immediately before dispensing. The filter holder (cat. No. XX4002500), which is part of the dispenser, can also be used by itself for in-line pressure filtration of gases.

Specifications Materials Stainless steel filter holder and filter support screens; nylon-coated aluminum dispenser handle; PTFE valve; fluoroelastomer filter-holder gaskets; polyethylene pressure tubing; stainless steel jet nozzle Filter Diameter, mm 25 Filtration Area, cm² 3.9 Prefilter Diameter, mm 22 (thick depth prefilter) Max Inlet Pressure, bar (psi) 3.5 (50) Fittings 1/4 in. NPTF to 1/4 in. Dimensions Approximately 229 mm (9 in.) from nozzle tip to end of

Analytical dispenser handle, with 1.8 m (6’) tubing

Ordering Information Description Qty/Pk Catalogue No. Collect Filterjet™ Solvent Dispenser, 25 mm 1 XX6702500 Replacement Parts Handle and Valve Assembly 1 XX6702501 Valve Seals, PTFE 4 XX6702502 Pressure Tubing with 1/4 in. NPT Adapters, PE 1 XX6702506 Tube Adapter, 1/4 in. NPTM to 1/4 in. Tube 1 XX6702507 Tubing, 6 mm O.D. x 3 m, polypropylene 1 XX6702508 Replacement Pats Kit for Filterjet™ Solvent Dispenser 1 XX67025RK* Gas Line Filter Holder, 25 mm, stainless steel 1 XX4002500 Replacement Parts Kit for Filter Holder 1 XX40025RK** Note: additional replacement parts available. Accessories Dispensing Pressure Vessel, 5 L 1 XX6700P05 Pressure Gauge, 0–7 bar 1 YY1301015 Ball Valve, 1/4 in. NPTF, stainless steel 1 YY2029348 Quick-release Nipple & Coupling, 1/4 in. NPTM 1 XX6700030 *Includes stainless steel jet nozzle, fan spray and cone spray **Includes (5) PTFE locking nut washers, (5) fluoroelastomer-A (2-021) O-rings, (5) stainless steel support screens

For more information visit: www.merckmillipore.com/ParticleMonitoring

142 www.merckmillipore.com Analytical Collect 143

for µm

sizes

1.0 µm

pore 0.45

levels,

housing

levels,

and

110 GWSC04501 50 GWSC04510 1 GWSC04550 10 GWSC10001 1 GWSC10010 GWSC50001 different

filter particulate

three

particulate high

with

for

0.45 1.0 5.0 Pore Size (µm) Size Pore Qty/Pk Catalogue No. erials minimize background during during background minimize erials µm normal

or elatively clean water elatively sample analysis sample mat conditions: variety suit a of water to 5.0 f r • Low-extractable • Available Applications Metals Dissolved Monitoring, Environmental Analysis in Water 600 76 102 4.1 (60) 0.7 (10) 3.8 (1) water air with ≥2070 mbar, water air with ≥690 mbar, water air with ≥345 mbar, HDPE Polyethersulfone connections; with threaded 1/8 in. NPTM, external I.D. tubing 3/8 in. up to for hose adapter stepped 4.1 (60)

certificate

includes

west detectable level (LDL) level detectable west capsule

indicating lo and 2 anions 67 metals analysis for Ordering Information Ordering Description Capsules Sampling Groundwater 5.0 µm Bubble Point at 23 °C Bubble Point 0.45 µm 1.0 µm Maximum Differential bar (psid) Pressure, at 10 psi, L/min (gpm) Rate Flow Water Height, mm PropertiesPerformance bar (psi) Pressure, Maximum Inlet Dimensions cm² Area, Filtration mm Diameter, Maximum Pressure, bar (psi) Maximum Pressure, Membrane Connections Inlet/Outlet Specifications Materials Housing www.merckmillipore.com For more information visit: www.merckmillipore.com/ParticleMonitoring visit: For information more Features & Benefits • Each EPA-approved for groundwater collection groundwater for EPA-approved capsules are sampling These groundwater collection of for method an EPA-approved analysis. metals dissolved to prior groundwater times four provides membrane The pleated disc than a 142 mm filter area filtration more throughput. higher and rates flow faster for Groundwater Sampling Capsules Sampling Groundwater ZHE Hazardous Waste Filtration System (90 mm) EPA-approved for EP Toxicity Test and TCLP Features & Benefits Applications • Allows for pressurization of filter without US EPA Extraction Procedure (EP) Toxicity Test introducing air, due to movable piston and Toxicity Characteristic Leaching Procedure design • Prevents loss of volatiles • Manual and automatic venting modes • System cannot be pressurized unless completely assembled, thereby preventing “shooting the piston” out of the unit accidentally

Specifications Materials 316 stainless steel body; molded polypropylene handwheels; fluoroelastomer-A O-rings Connections Top Plate 1/8 in. NPTF upstream port for two-way SST valve with 1/8 in. NPTF for Luer fitting Bottom Plate 1/4 in. NPTF port for 1/4 in. elbow or 1/4 in. x 1/8 in. NPT T-fitting; 1/4 in. NPTF port for pressure relief valve Maximum Pressure, bar (psi) 3.5 (50) Do your waste samples contain volatile Dimensions substances, such as isopropanol, acetone or Filter Diameter, mm 90 xylene? Then use our ZHE (Zero Head Space Filtration Area, cm² 64 Prefilter Diameter, mm 90

Analytical Extractor) Hazardous Waste Filtration system, which is approved by the US Environmental Diameter, cm 15.9 27.9 Protection Agency (EPA) as an apparatus for Height, cm Shipping Weight, kg 6.8 Toxicity Characteristic Leaching Procedure Piston Break Force, bar 0.35 – 0.7 (TCLP) measurement. Collect

Ordering Information Description Qty/Pk Catalogue No. Description Qty/Pk Catalogue No. ZHE Hazardous Waste System 1 YT30090HW Accessories Replacement Parts ZHE Tedlar Bag, 1 liter 10 YT3009016 Fitting Luer 1/8 in. NPTF 1 YT3009002 ZHE Tight Syringe, 50 mL 1 YT3009015 2-way Valve, 1/8 in. NPTM, stainless steel 1 XX2702512 Dispensing Pressure Vessel, 5 L 1 XX6700P05 Hand Knob 1 YY2214257 Connector Kit Components O-ring (2-235), 90 mm, fluoroelastomer 6 YY2209068 Ball Valve, 1/4 in. NPTF, stainless steel 1 YY2029348 Support Screen, 90 mm, stainless steel 1 YY2209064 Vent/Relief Valve, 9 bar 1 XX6700024 Cylinder O-rings 3 XX6700010 Pressure Gauge, 0–7 bar 1 YY1301015 Piston with O-rings, wiper seal 1 YT3009001 Quick-release Nipple & Coupling, 1/4 in. NPTM 1 XX6700030 Piston O-rings, wiper seal 3 YT3009003 Quick-disconnect Female Swagelok® Fitting 1 YT3009013 Street Elbow, 1/4 in. NPTF to M 1 XX6700104 Hex Nipple, 1/4 in. NPTM 51 mm, stainless steel 1 XX6700125 Quick-disconnect Female Swagelok® 1 YT3009013 Tube Adapter, 1/4 in. NPTM to 1/4 in. Tube 1 XX6702507 Quick-disconnect Male Swagelok® 1 YT3009014 Tubing, 6 mm O.D. x 3 m, polypropylene 1 XX6702508 Vent/Relief Valve, 9 bar 1 XX6700024 Adapter, 1/8 in. NPTF to M Luer-Lok®, chrome plated 1 XX3002567 Gauge, 0-100 psi 1/8 in. NPT 1 P16938 Note: For rotary agitator and accessories, see Hazardous Waste Filtration System (142 mm). Quick Connector, female, 1/8 in. NPT 1 P16940 ZHE Holder, piston pusher 1 13899

For more information visit: www.merckmillipore.com/ParticleMonitoring

144 www.merckmillipore.com Analytical Collect 145 1 YT310RAHW 1 XX6700035 Qty/Pk Catalogue No. I.D.

1/2 in.

Use,

coating

samples Pressure

for

PTFE

bulky

of Toxicity

with Description Accessories fluoroelastomer 1-1/2 in., PTFE-coated Gasket, Tubing Filters Membrane AP40, 142 mmGlass Fiber Binder, without Filter 10 YY2004057 142 mm APFF, Glass Fiber Binder, without Filter 50Rotary Agitator 50 115 V/60 Hz Agitator, Rotary AP4014250* TCLP analysis. for 1311 Method in EPA recognized are *AP40 filters APFF14250** 1311. Method EPA for specifications meet **APFF filters

cleaning

and

holder easy

Test

for

introduction

interior

Toxicity

easy

acteristic Leaching Procedure Leaching acteristic EPA hrough wide entry hrough port and removable o prevent heavy metal contamination metal heavy o prevent

t t plate top • Allows Applications US Char • Disassembles Features& Benefits • Contains 13 YT3014257 1 YY2214251 YY2214252 110 YY2214257 1 YY2004055 4 YY2004045 1 YY2214265 YY2214253 Qty/Pk Catalogue No.

316 stainless steel; aluminum legs; molded polypropylene legs; molded polypropylene aluminum 316 stainless steel; handwheels; PTFE seal for 14 mm and adapters 1-1/2 in. sanitary clamps with flange port vent/relief for (9/16 in.) I.D. hose; 1/8 in. NPTF upstream 5.2 (75) 43 (17) 18.4 142 124; type filter AP depth 97 1.5 12.3 (27.1)

the

for

the

for

with

PTFE-coated specifically

collaboration

Support,

designed

vironmental Protection Agency (EPA), the the (EPA), Agency Protection vironmental A Extraction Procedure (EP) Toxicity Test Test (EP) Toxicity Procedure A Extraction Legs with Caps, 3/16 in. wrench Legs with 142 mm Holder for Handwheel Wrench Hazardous Waste Filtration 142 mm System, Waste Hazardous PartsReplacement Hand Knob 1 YT30142HW Ordering Information Ordering Description Silicone Gasket 1-1/2 in. TC 1-1/2 in. TC Clamp (2-251), silicone O-ring (2-251), PTFE O-ring Underdrain Fittings bar (psi) Pressure, Maximum Operating Dimensions Height, cm cm Diameter, mm Diameter, Filter mm Diameter, Prefilter cm² Area, Filtration L Volume, (lb) kg Shipping Weight, Materials Specifications www.merckmillipore.com For more information visit: www.merckmillipore.com/ParticleMonitoring visit: For information more and Toxicity Characteristic Leaching Procedure Procedure Leaching Characteristic and Toxicity liquid solid and separates The device (TCLP). analysis. for samples phases of waste En system EP EPA approved for TCLP for approved EPA contain non-volatile samples waste If your use substances or metals, or semi-volatile Filtration System Waste Hazardous this extraction, separation, and filtration. for Developed Hazardous Waste Filtration System (142 mm) (142 System Filtration Waste Hazardous 146 www.merckmillipore.com Analytical Sample Preparation

Collect Prepare Test Proper preparation of samples, solvents and buffers, using our solutions for filtration and solid phase extraction, generates page 125 higher quality, more consistent page 207 results. These strategies to reduce contaminating sample components also increase analytical instrument uptime and prolong analytical column life. Application Spotlight

Solutions for the Complete HPLC/UHPLC Workflow

Glass Filter Holders, Pages 127-128 Ultra Pure Water, Page 215

Membrane Disc Filters, Pages 172-181, 184 Columns, Pages 208-211

Samplicity Filtration System, Page 164 Chromatogram Analytical

Millex® Syringe Filters,

Prepare Pages 152-163 Solid-Phase Extraction, Page 199

Obtain more information about your mole- Mobile Phase Preparation Membranes that display the highest particle cules of interest and increasingly fine sepa- retention tend to be the most effective at Contaminating solutes may contribute to ration of complex solutions using the newest minimizing backpressure. Polypropylene baseline variability and poor chromatographic liquid chromatography technologies, such membranes exhibit poor particle retention, performance when bottled water instead of as high performance liquid chromatography and therefore filtering UHPLC mobile phases freshly delivered water is used to prepare the (HPLC) and ultra-high performance liquid through polypropylene is the least effective mobile phases. Using Milli-Q® water purifi- chromatography (UHPLC), that feature high for reducing backpressure buildup. In con- cation systems will ensure that your mobile sensitivity and throughput. To benefit from trast, filtering the mobile phase through PTFE phases are free of organic contaminants and these advanced separation systems, you need membranes, which show excellent particle deliver the best, most reproducible chromato- cutting-edge columns, high purity reagents retention, enable the UHPLC system to run graphic results. and state-of-the-art preparation tools for without significant backpressure buildup. higher purity samples and cleaner mobile Membrane filtration removes contaminating phases. Merck Millipore’s solutions for liquid particles from solvents and mobile phases, chromatography support the entire workflow, increasing column life, minimizing backpres- delivering higher quality, more consistent sure, and preventing system failure. That’s results, increased instrument uptime and why most HPLC/UHPLC instrument manufac- prolonged column life. turers recommend filtration of mobile phases using either 0.45 or 0.20 μm filters.

148 www.merckmillipore.com Application Spotlight

6000 Figure0.45 µm 1. Polypropylene Filtration through (Vendor 0.2B) µm hydrophilic PTFE Millex® filters prevents 0.2 µm Polypropylene (Vendor B) 5500 ΔP = 2198 backpressure buildup on a UHPLC system. Water0.45 µm and Hydrophilic acetonitrile PTFE were Millex® passed ﬁlter through 5000 polypropylene0.2 µm Hydrophilic or PTFE PTFE syringe Millex® filters ﬁlter (as ΔP = 1529 indicated in legend), then used 1:1 (v/v) to 4500 prepare the mobile phase for UHPLC. The system was run at 0.25 mL/min for 600 min 4000 with backpressure recorded every 50 min. ΔP represents total change in backpressure 3500 after 600 min.

System Backpressure (PSI) Backpressure System ΔP = - 29 3000 6000 0.45 µm Polypropylene (Vendor B) ΔP = -22 0.2 µm Polypropylene (Vendor B) 5500 2500 ΔP = 2198 0.45 µm Hydrophilic PTFE Millex® ﬁlter 5000 0.2 µm Hydrophilic PTFE Millex® ﬁlter 2000 ΔP = 1529 0 100 200 300 400 500 600 700 4500 Time (minutes) 4000

3500

System Backpressure (PSI) Backpressure System Reduce signal-to-noise ratios and maintainΔP = - 29 Sample Preparation3000 Analytical Separation clean baselines by filtering samples ΔwithP = -22 Sample preparation prior to analysis helps to Columns Analytical 2500 Millex® syringe filters, Millex Samplicity® Fil- bring a sample to a format that is compatible ters, or MultiScreen® Filter Plates, depending For reliable, reproducible analytical separa- with the analytical2000 technique, reduces sample 0 100 200on your 300 throughput 400 needs. 500 With their 600 broad 700 tions, even for the most challenging analyses, complexity, removes interfering impurities chemicalTime (minutes) compatibility, low holdup volumes, choose from a comprehensive range of from matrix and thereby concentrates the and consistent quality, Millex® filters are high-quality liquid chromatography columns. analyte prior to analysis. A typical sample for ideal for preparing samples for HPLC/UHPLC Thanks to their unique, patented, monolithic Prepare HPLC/UHPLC needs to be particle-free and analysis. silica technology, our Chromolith® columns completely soluble in the solvent compatible allow you to perform ultra-fast and robust with the chromatography system. In addition to filtration, you can use solid separations using standard HPLC systems. phase extraction with Lichrolut® columns For all polar and hydrophilic compounds, the Clean Sample or liquid-liquid extraction with the help of proprietary zwitterionic SeQuant® ZIC®-HILIC Clean Sample EXtrelut® sorbent to reduce the complexity technology provides straightforward HPLC of your sample, facilitating analysis and separations with high flexibility in the selec- improving reproducibility. These tools are not tion of separation conditions. The optimally only highly efficient, but they are also easy to balanced selectivity of Purospher® columns use and deliver high recoveries. makes them the perfect choice for reversed phase HPLC and UHPLC method development in a wide variety of labs.

Raw Sample Raw Sample

www.merckmillipore.com 149 Non-Sterile Millex® Syringe Filter Selection Guide

Process Volume Membrane Housing Diameter, mm (hold-up) Pore Size Page Non-sterile Millex® Syringe Filters Polypropylene 13 10 mL (≤ 15 µL) 0.22 159 with Millipore Express PLUS® (PES) Membrane 0.45 Fastest flow, high throughput 33 100 mL (≤ 80 µL) 0.22 0.45 Non-sterile Millex® Syringe Filters HDPE 4 1 mL (≤10 µL) 0.22 152 with Durapore® (PVDF) Membrane 0.45 Low protein binding Polypropylene 13 10 mL (≤ 15 µL) 0.22 0.45 PVC 25 100 mL (≤ 100 µL) 5.0 Polypropylene 33 100 mL (≤ 80 µL) 0.22 0.45 Non-sterile Millex® Syringe Filters Polypropylene 13 10 mL (≤ 15 µL) 0.20 157 with Nylon Membrane 0.45 Broad chemical compatibility 33 100 mL (≤ 80 µL) 0.20 0.45 Non-sterile Millex® Syringe Filters with LCR HDPE 4 1 mL (≤10 µL) 0.20 153 (Hydrophilic PTFE) Membrane 0.45 Lowest extractables and excellent solvent resistance 13 10 mL (≤ 25 µL) 0.20 0.45 25 100 mL (≤ 100 µL) 0.20 0.45 Analytical Non-sterile IC Millex® Syringe Filters with LCR HDPE 13 10 mL (≤ 25 µL) 0.20 156 (Hydrophilic PTFE) Membrane 0.45 Sample preparation for ion chromatography 25 100 mL (≤ 100 µL) 0.20 0.45

Prepare Non-sterile Millex® Syringe Filter with Fluoropore™ HDPE 4 1 mL (≤10 µL) 0.20 155 (Hydrophobic PTFE) Membrane 0.45 Excellent solvent resistance Polypropylene 13 10 mL (≤ 15 µL) 0.20 0.45 HDPE 25 100 mL (≤ 100 µL) 0.20 0.45 5.0 Non-sterile Millex® Syringe Filters PVC 25 100 mL (≤ 100 µL) 0.22 160 with MCE Membrane 0.45 General purpose filtration 0.80 Automation-Compatible & High Particulate Filtration Glass Fiber HDPE 25 100 mL (<250 µL) 1.0 162, 163 LCR (Hydrophilic PTFE) Membrane with graduated HDPE 25 100 mL (<250 µL) 0.20 multi-layer glass fiber prefilter 100 mL (<250 µL) 0.45 Durapore® (PVDF) Membrane with graduated HDPE 25 100 mL (<250 µL) 0.45 multi-layer glass fiber prefilter Nylon Membrane with graduated multi-layer glass fiber HDPE 25 100 mL (<250 µL) 0.20 prefilter 100 mL (<250 µL) 0.45

150 www.merckmillipore.com Non-Sterile Millex® Syringe Filter Selection Guide s ification of -M hplc c

Membrane Pore Size Dissolution Testing IC HPLC U L Clar Particle-Laden Solutions of Clarification Solutions Aqueous of Clarification Solutions Organic of Clarification and Aqueous Solutions Organic of Clarification Proteinaceous Solutions Filtration Vent Millipore Express® PLUS 0.22 ● ● ● ● ● ● ● (PES) Membrane 0.45 ● ● ● ● ● ● Fastest flow, high throughput Durapore® (PVDF) 0.22 ● ● ● ● ● Membrane 0.45 ● ● ● ● ● Low-protein binding 5.0 ● ● Nylon Membrane 0.20 ● ● ● ● ● ● Broad chemical compatibility 0.45 ● ● ● ● ● ● LCR (Hydrophilic PTFE) 0.20 ● ● ● ● ● ● ● Membrane 0.45 ● ● ● ● ● ● ● Lowest extractables and excellent solvent resistance IC Millex® Syringe Filters 0.20 ● ● ● ● with LCR (Hydrophilic 0.45 ● ● ● ● PTFE) Membrane Analytical Sample preparation for ion chromatography Fluoropore™(Hydrophobic 0.20 ● ● ● PTFE) Membrane 0.45 ● ● ● Excellent solvent resistance 5.0 ● ● MCE Membrane 0.22 ● ● Prepare General purpose filtration 0.45 ● ● 0.80 ● ● ● Glass Fiber Membrane Glass Fiber ● Automation-Compatible & High Particulate Filtration Glass Fiber 1.0 ● ● ● ● ● LCR (Hydrophilic PTFE) 0.20 ● ● ● ● ● ● ● ● Membrane with graduated 0.45 ● ● ● ● ● ● ● ● multi-layer glass fiber prefilter Durapore® (PVDF) Membrane 0.45 ● ● ● ● ● ● with graduated multi-layer glass fiber prefilter Nylon Membrane with 0.20 ● ● ● ● ● ● ● graduated multi-layer glass 0.45 ● ● ● ● ● ● ● fiber prefilter

www.merckmillipore.com 151 Non-Sterile Millex® Syringe Filters with Durapore® Membrane Low protein binding Non-sterile Millex® syringe filters with • Available in 0.22 μm and 0.45 μm pore hydrophilic Durapore® (PVDF) membrane sizes and three diameters to suit your provide high flow rates and throughput, low application needs levels of extractables and broad chemical • Housing materials are either polypropylene compatibility. Hydrophilic PVDF membranes or high density polyethylene (HDPE), bind far less protein than nylon, nitrocellulose offering low levels of extractables and membranes, or PTFE membranes. Syringe broad chemical compatibility filters are available in two pore sizes and three diameters to optimize results. Applications Sample Filtration Prior to UHPLC, HPLC and Features & Benefits Mass Spec; Solvent Filtration; Filtration of • Low protein binding to minimize interaction Biological Samples and Protein Solutions with your sample and maximize recovery

Product Performance Analytical Prepare

Specifications 4 mm Millex® Filters 13 mm Millex® Filters 25 mm Millex® Filters 33 mm Millex® Filters Housing Material HDPE Polypropylene PVC Polypropylene Housing Color Natural Yellow band Clear Yellow band Membrane Material Hydrophilic Durapore® (PVDF) Hydrophilic Durapore® (PVDF) Hydrophilic Durapore® (PVDF) Hydrophilic Durapore® (PVDF) Pore Sizes Available, µm 0.22, 0.45 0.22, 0.45 5.0 0.22, 0.45 Inlet Fittings Female Luer-Lok® Female Luer-Lok® Female Luer-Lok® Female Luer-Lok® Outlet Fittings Male LuerSlip™, stepped Male Luer-Slip™ Male Luer-Slip™ Male Luer-Slip™ Filtration Area, cm2 0.1 0.8 3.9 4.5 Process Volume, mL 1 ≤10 ≤100 ≤100 Hold-up Volume, µL <10 ≤15 after air purge <100 ≤80 after air purge Maximum Pressure, bar (psi) 14 (200) 10 (150) 5.2 (75) 8.6 (125) Maximum Temperature, °C 45 45 45 45

152 www.merckmillipore.com Analytical Prepare 153

organic

pore

and

µm

optimizing

0.45

aqueous

and

Solutions

both µm

1001000100 SLGVR04NL 1000 SLGVR04NK 100 SLHVR04NL 100 SLHVR04NK 1000 SLGVX13NL 100 SLGVX13TL* 100 SLGVX13NK 1000 SLHVX13NL 50 SLHVX13TL* 250 SLHVX13NK 1000 SLGV033NS 50 SLGV033NB 250 SLGV033NK 1000 SLHV033NS 250 SLHV033NB SLHV033NK SLSV025NB

extractables,

of oviding broad chemical chemical broad oviding 0.20

with

Organic

level

and

kground levels of sensitve analyses like analyses like of sensitve levels kground es and three diameters to suit your your suit to diameters es and three application needs UHPLC and LC-MS UHPLC siz bac solutions, pr compatibility flexibility and the lab in Applications and HPLC UHPLC, to Filtration Prior Sample of Filtration; Clarification Mass Spec; Solvent Aqueous • Compatible Features & Benefits • Lowest • Available 0.22 0.45 0.22 0.45 0.22 0.45 5.0 Diameter (mm)Diameter (µm) Size Pore Qty/Pk Catalogue No. Outlet

*Tube Millex®-HV Syringe FilterMillex®-HV Syringe 4 FilterMillex®-GV Syringe 13 FilterMillex®-HV Syringe 13 FilterMillex®-GV Syringe 33 FilterMillex®-HV Syringe 33 Millex®-SV Filter Syringe 25 Millex®-GV Syringe FilterMillex®-GV Syringe 4 Ordering Information Ordering Description www.merckmillipore.com Hydrophilic PTFE membranes provide the the provide PTFE membranes Hydrophilic of extractables level and broad lowest aqueous compatibility both chemical with PTFE solutions. Hydrophilic and organic samples preparing ideal for are membranes liquid to and mobile phases prior or mass spectrometry (LC) chromatography filter (MS) analysis and can be used to wetting. prior solutions without aqueous sizes pore available in two are filters Syringe results. optimize to diameters and three a low-extractable, The housing is made from (HDPE). high density polyethylene Non-Sterile Millex® Syringe Filters with Hydrophilic PTFE Membrane PTFE Hydrophilic with Filters Millex® Syringe Non-Sterile resistance solvent of extractables and excellent level Lowest For more information visit: www.merckmillipore.com/NSmillex visit: For information more Product Performance

Specifications 4 mm Millex® Filters 13 mm Millex® Filters 25 mm Millex® Filters Housing Material HDPE HDPE HDPE Housing Color Natural Natural Natural Analytical Membrane Material Hydrophilic PTFE Hydrophilic PTFE Hydrophilic PTFE Pore Sizes Available, µm 0.20, 0.45 0.20, 0.45 0.20, 0.45 Inlet Fittings Female Luer-Lok® Female Luer-Lok® Female Luer-Lok® Outlet Fittings Male Luer-Slip™, stepped Male Luer-Slip™ Male Luer-Slip™ Filtration Area, cm2 0.1 0.65 3.9 Prepare Process Volume, mL 1 ≤10 ≤100 Hold-up Volume, µL <10 ≤25 after air purge <100 after air purge Maximum Pressure, bar (psi) 14 (200) 7 (100) 7 (100) Maximum Temperature, °C 45 45 45

Ordering Information Description Diameter (mm) Pore Size (µm) Qty/Pk Catalogue No. Millex®-LG Syringe Filter 4 0.20 100 SLLGR04NL Millex®-LH Syringe Filter 4 0.45 100 SLLHR04NL 1000 SLLHR04NK Millex®-LG Syringe Filter 13 0.20 100 SLLGH13NL 1000 SLLGH13NK Millex®-LCR Syringe Filter 13 0.45 100 SLCR013NL 100 SLCRT13NL* 1000 SLCR013NK Millex®-LG Syringe Filter 25 0.20 50 SLLGH25NS 250 SLLGH25NB 1000 SLLGH25NK Millex®-LCR Syringe Filter 25 0.45 50 SLCR025NS 250 SLCR025NB 1000 SLCR025NK *Tube Outlet

For more information visit: www.merckmillipore.com/NSmillex

154 www.merckmillipore.com Analytical Prepare

155 Sterilization

Filtration,

Vent

Solutions,

Applications of and Clarification Fine Particle Removal Organic of Gases

excellent

three

and

Natural 0.20, 0.45, 5.0 25 mm Millex® Filters 3.9 ≤100 <100 after air purge* 7 (100) 45 sizes

pore compatibility

two

chemical ters to suit your application needs application suit your to ters

vent resistance with organic solutions organic with resistance vent 0.20, 0.45 Polypropylene band Red HDPE ≤10 ≤15 after air purge 10 (150) 45 0.8 13 mm Millex® Filters sol diame eatures & Benefits eatures optimize results. The housing is made from a from The housing is made results. optimize density high low-extractable, polyethylene (HDPE) or polypropylene. F • Broad • Available

and purge

air

µm

after Hydrophobic PTFEHydrophobic 0.20, 0.45 Female Luer-Lok® PTFE Hydrophobic steppedMale Luer-Slip™, PTFE Hydrophobic Male Luer-Slip™ Female Luer-Lok® Male Luer-Slip™ Female Luer-Lok® HDPE Natural 1 <10 14 (200) 45 4 mm Millex® Filters 0.1

0.20 diameters

<300

three

volume

and 2

available

are sizes

hold-up

pore

device filters

µm µm

5.0

Pore Sizes Available, µm Available, Sizes Pore Fittings Outlet cm Area, Filtration Membrane Material Membrane Fittings Inlet * Housing Color Housing Material Specifications Process Volume, mL Volume, Process µL Hold-up Volume, bar (psi) Maximum Pressure, °C Maximum Temperature, www.merckmillipore.com Product Performance Product 0.45 Excellent solvent resistance solvent Excellent with filters Millex® syringe Non-sterile (PTFE) membrane Fluoropore™ hydrophobic compatibility are and chemical broad provide solvents. in organic samples filtering ideal for can filters syringe Fluoropore™ Hydrophobic of back-streaming prevent also be used to appications. in venting moisture atmospheric Syringe Non-Sterile Millex® Syringe Filter with Fluoropore™ Membrane Membrane Fluoropore™ with Filter Millex® Syringe Non-Sterile Ordering Information Description Diameter (mm) Pore Size (µm) Qty/Pk Catalogue No. Millex®-FG Syringe Filter 4 0.20 100 SLFGR04NL Millex®-FH Syringe Filter 4 0.45 100 SLFHR04NL Millex®-FG Syringe Filter 13 0.20 100 SLFGX13NL 100 SLFGX13TL* 1000 SLFGX13NK Millex®-FH Syringe Filter 13 0.45 100 SLFHX13NL 100 SLFHX13TL* 1000 SLFHX13NK Millex®-FG Syringe Filter 25 0.20 50 SLFG025NS 250 SLFG025NB 1000 SLFG025NK Millex®-FH Syringe Filter 25 0.45 50 SLFH025NS 250 SLFH025NB 1000 SLFH025NK Millex®-LS Syringe Filter 25 5.0 50 SLLS025NS *Tube Outlet

For more information visit: www.merckmillipore.com/NSmillex Analytical Non-Sterile IC Millex® Syringe Filters with Hydrophilic PTFE Membrane Prepare Sample preparation for ion chromatography Non-sterile ion chromatography (IC) Millex® Features & Benefits syringe filters with hydrophilic PTFE • Lowest level of IC extractables, optimizing membrane provide the lowest IC extractable background levels in sensitive IC analyses levels and broad chemical compatibility with • Compatible with both aqueous and organic both aqueous and organic solutions. Syringe solutions, offering broad chemical filters are available in two pore sizes and two compatibility and flexibility in the lab diameters to optimize results. The housing is • Each lot is certified to contain low ion made from a low-extractable, high density extractable levels polyethylene (HDPE). In addition, each unit is • Available in 0.20 µm and 0.45 µm pore individually packaged to minimize the risk of sizes and two diameters to suit your extraneous ionic contamination. Lot release application needs criteria include bubble point, flow rate, housing pressure, downstream particles, and IC levels. A certificate of quality with complete Applications specifications is included in each box. Ion Chromatography

156 www.merckmillipore.com Analytical Prepare 157

organic

pore

and µm

0.45

100 <100 after air purge 7 (100) 45 3.9 HDPE Female Luer-Lok® Male Luer-Slip™ 25 mm Millex® Filter aqueous

and

Solutions 10050 SLLHC13NL SLLHC25NS 10050 SLLGC13NL SLLGC25NS

µm both

0.20 oviding broad chemical chemical broad oviding with

Organic m) Qty/Pk Catalogue No.

μ in

and

es and two diameters to suit your suit your to diameters es and two 0.45 0.20 application needs solutions, pr siz compatibility and flexibilitythe lab in Applications and HPLC UHPLC, to Filtration Prior Sample of Filtration; Clarification Mass Spec; Solvent Aqueous Features & Benefits • Compatible • Available <25 after air purge 7 (100) 45 10 13 mm Millex® Filter 0.65 HDPE Female Luer-Lok® Male Luer-Slip™ 25 25 Diameter (mm)Diameter ( Size Pore 2 IC Millex®-LH Syringe FilterIC Millex®-LH Syringe 13 Description Filter Syringe IC Millex®-LG 13 Ordering Information Ordering Hold-up Volume, µL Hold-up Volume, (psi) bar Pressure, Maximum Inlet °C Temperature, Maximum Operating Process Volume, mL Volume, Process Inlet Fittings Inlet Fittings Outlet cm Area, Filtration Housing Material Specifications www.merckmillipore.com Broad chemical compatibility chemical Broad with filters Millex® syringe Non-sterile compatible are membrane nylon hydrophilic making them of solvents, range a broad with aqueous of most filtration sample ideal for laboratory. the across solvents and organic sizes pore available in two are filters Syringe results. optimize to diameters and three polypropylene, The housing is made from of extractables level and broad offering a low compatibility. chemical Non-Sterile Millex® Syringe Filters with Nylon Membrane Nylon with Filters Millex® Syringe Non-Sterile For more information visit: www.merckmillipore.com/NSmillex visit: For information more Specifications 13 mm Millex® Filters 33 mm Millex® Filters Housing Material Polypropylene Polypropylene Housing Color Purple band Purple band Membrane Material Hydrophilic Nylon Hydrophilic Nylon Pore Sizes Available, µm 0.20, 0.45 0.20, 0.45 Inlet Fittings Female Luer-Lok® Female Luer-Lok® Outlet Fittings Male Luer-Slip™ Male Luer-Slip™ Filtration Area, cm2 0.8 4.5 Process Volume, mL ≤10 ≤100 Hold-up Volume, µL ≤15 after air purge ≤80 after air purge Maximum Pressure, bar (psi) 10 (150) 8.6 (125) Maximum Temperature, °C 45 45

Ordering Information Description Diameter (mm) Pore Size (µm) Qty/Pk Catalogue No. Millex®-GN Syringe Filter 13 0.20 100 SLGNX13NL 100 SLGNX13TL* 1000 SLGNX13NK Millex®-HN Syringe Filter 13 0.45 100 SLHNX13NL 100 SLHNX13TL* Analytical 1000 SLHNX13NK Millex®-GN Syringe Filter 33 0.20 50 SLGN033NS 250 SLGN033NB 1000 SLGN033NK

Prepare Millex®-HN Syringe Filter 33 0.45 50 SLHN033NS 250 SLHN033NB 1000 SLHN033NK *Tube Outlet

For more information visit: www.merckmillipore.com/NSmillex

158 www.merckmillipore.com Analytical Prepare 159

eraction pore

provides int

e Aqueous µm

0.45

minimiz

membrane

o and t

PES

µm 1001000 SLGPX13NL SLGPX13NK 1001000 SLHPX13NL SLHPX13NK 502501000 SLGP033NS SLGP033NB SLGP033NK 502501000 SLHP033NS SLHP033NB SLHP033NK

33 mm Millex® Filters Polypropylene band Green PES Millipore Hydrophilic PLUS Express® 0.22, 0.45 Female Luer-Lok® Male Luer-Slip™ 4.5 ≤100 ≤80 after purge air 8.6 (125) 45 Clarification

binding 0.22

otein Filtration, pr

h your sample and maximize recovery and maximize sample h your es and two diameters to suit your suit your to diameters es and two ast filtration for large sample volumes sample for large filtration ast 0.22 0.45 0.22 0.45 wit f siz application needs Buffer Features& Benefits • Low • High-throughput Solutions Applications • Available Polypropylene Green band Green PES Millipore Hydrophilic PLUS Express® 0.22, 0.45 Female Luer-Lok® Male Luer-Slip™ 13 mm Millex® Filters 0.8 ≤10 ≤15 after purge air 10 (150) 45 Diameter (mm)Diameter (µm) Size Pore Qty/Pk Catalogue No. 2 Description FilterMillex®-GP Syringe 13 Ordering Information Ordering Housing Material Specifications Housing Color Material Membrane µm Available, Sizes Pore Fittings Inlet Fittings Outlet cm Area, Filtration Process Volume, mL Volume, Process µL Hold-up Volume, bar (psi) Maximum Pressure, °C Maximum Temperature, Millex®-HP Syringe FilterMillex®-HP Syringe 13 Millex®-GP Syringe FilterMillex®-GP Syringe 33 Millex®-HP Syringe FilterMillex®-HP Syringe 33 www.merckmillipore.com For more information visit: www.merckmillipore.com/NSmillex visit: For information more Fastest flow, high throughput high flow, Fastest with filters Millex® syringe Non-sterile polyethersulfone PLUS Express® Millipore of ultrafast filtration provide (PES) membrane low fast-filtering, solutions. This aqueous by is preferred membrane protein-binding available are filters Syringe researchers. many to diameters and two sizes pore in two is made from The housing results. optimize of level offering a low polypropylene, compatibility. chemical extractablesbroad and Non-Sterile Millex® Syringe Filters with PES Membrane PES Membrane with Filters Millex® Syringe Non-Sterile Non-Sterile Millex® Syringe Filters with MCE Membrane Broad application and research use Non-sterile Millex® syringe filters with Features & Benefits hydrophilic mixed cellulose esters (MCE) • MCE is the most widely used and membrane are widely used in general referenced general purpose membrane analytical and research applications. Syringe • Available in 0.22 µm, 0.45 µm and 0.80 µm filters are available in three pore sizes to pore sizes to suit your application needs optimize results. The housing is made from a low-extractable polyvinylidene chloride (PVC). Applications Clarification of Aqueous Solutions, General Laboratory Filtration

Specifications Ordering Information Housing Material PVC Description Diameter (mm) Pore Size (µm) Qty/Pk Catalogue No. Housing Color Clear top/blue base* Millex®-GS 25 0.22 250 SLGS025NB Syringe Filter Membrane Material Hydrophilic MCE 1000 SLGS02510 Pore Sizes Available, µm 0.22, 0.45, 0.80, prefilter Millex®-HA 25 0.45 100 SLHA025NB Syringe Filter Inlet Fittings Female Luer-Lok® 1000 SLHA02510 Outlet Fittings Male Luer-Slip™ Millex®-AA 25 0.80 250 SLAA025NB Syringe Filter Filtration Area, cm2 3.9 1000 SLAA025NK Process Volume, mL 100 Millex®-AP 25 NA 50 SLAP02550 Syringe Filter Hold-up Volume, µL ≤100** Maximum Pressure, bar (psi) 5.2 (75)

Analytical Maximum Temperature, °C 45 For more information visit: www.merckmillipore.com/NSmillex *0.8 µm device has clear top with green base. Glass prefilter device is natural colored. **Hold-up volume does not apply to glass prefilter device. Prepare

In-Line Millex® Filter Units (25 mm and 50 mm) Ideal for in-line sterilization of gases and venting sterile containers

Millex® syringe filter units with hydrophobic Features & Benefits Fluoropore™ or Millipore Express® PLUS PES • Specialized filter units protect hemodialysis membrane are ideal for in-line sterilization of transducers from blood and moisture gases and venting sterile containers, and filters • 50 mm Millex® units ideal for vacuum line with Fluoropore™ membrane can also be used protection for sterilizing or clarifying organic solutions. • All Millex® Hydrophobic PTFE units are There are also specialized filter units to protect bi-directional in flow hemodialysis transducers from blood and moisture. The 50 mm Millex® filter units are Applications especially useful for vacuum line protection. Sterilizing Gases, Vacuum Line Protection, All units are bi-directional. Venting Sterile Containers, Sterilizing and Clarifying Organic Solutions

160 www.merckmillipore.com Analytical Prepare 161 SLFA05010 SLFA05000 100 10 50 SLFG025LS 50 SLFGL25BS 50 SLFG02550 50 SLGVS25US 50 SLGVS25PS 50 SLGVS25XS 10 SLFG65010 100 SLFG65000 10100 SLFG75010 10 SLFG75000 SLFG85010 100 SLFG85000 10 SLFH05010 10 SLFH05000 10 SLFG05010 100 SLFG05000 10 SLFG55010 10 SLGP05010 10 SLGPB5010 Autoclavable Autoclavable oxide Ethylene oxide Autoclavable Ethylene oxide oxide oxide Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Autoclavable Gamma ir- radiated Gamma ir- radiated Sterilization Qty/Pk Catalogue No. 45 Acrylic 19.6 4.1 (60) Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female Fitting Outlet Fitting 1/8 in. NPTM 1/8 in. NPTM 1/8 in. NPTM 1/8 in. NPTM with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female 1/8 in. NPTM Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped Luerfemale Slip™ interior and filling bell 121 Polypropylene 19.6 4.1 (60) Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female Female Luer-Lok® Male Luer-Lok® Female Luer-Lok®Female Luer-Lok® Male Luer Slip™ Female Luer-Lok® Male Luer-Lok® Ethylene Male Luer Slip™ Female Luer-Lok® Female Luer-Slip™ Male Luer Slip™ Male Luer Slip™ Ethylene Ethylene Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female 1/8 in. NPTM 1/8 in. NPTM with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female with hose barb Stepped interior Luer-Slip™ female Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female Stepped hose barb with with hose barb Stepped interior Luer-Slip™ female PTFE PVDF PTFE PTFE PTFE PTFE PTFE PTFE PTFE Express® Express® PES PLUS Membrane Inlet Fitting 45 PVC 25 mm Millex® Units Membrane PTFE with 50 mm Millex® Units 3.9 PES Membrane with 50 mm Millex® Units 5.2 (75) C o 1 Fluoropore™ 0.22 Durapel™ Pore Size Size Pore (µm) 0.2 Fluoropore™ 0.2 Fluoropore™ 0.20.2 Fluoropore™ Fluoropore™ 0.45 Fluoropore™ 0.2 Fluoropore™ 0.2 Fluoropore™ 0.22 Millipore 2 25 50 25 (mm) 50 50 50 50 50 50 50

50 50 50 50 50 50 50 50 Housing Material Specifications Filtration Area, cm Area, Filtration Maximum Inlet Pressure, bar (psi) bar Pressure, Maximum Inlet Temperature, Maximum Operating Millex®-GP Millex®-FG Millex®-FG Millex®-FG Millex®-FH Millex®-FA Millex®-FG Millex®-FG Transducer Protectors Transducer Dualex™ Ultra Unit Filter Filter Unit Filter Millex®-FG Unit Filter Ordering Information Ordering Description Diameter Filter Filter Unit Filter Filter Unit Filter Unit Filter Unit Filter Filter Unit Filter Unit Filter Filter Unit Filter www.merckmillipore.com 25 mm Automation-Compatible Filter Units High-throughput filtration Features & Benefits Applications • Domed housing ensures reliable delivery Drug Dissolution Testing, Automated Sample of filters Preparation, Clarification of Solutions • Pressure-resistant housing resists bursting Containing High Particle Levels • Luer-Lok® connection optimized for precise alignment and fit • Available in either bulk or delivery tubes for use with automated filter-changing systems Automation-compatible, non-sterile Millex® syringe filter units provide efficient filtration Specifications for dissolution testing and other automated Housing Material HDPE sample preparation applications, especially for Inlet Fittings Female Luer-Lok® pharmaceutical product analysis. These filters Outlet Fittings Male Luer-Slip™ feature high-density polyethylene housing Filtration Area, cm² 3.9 and a wide choice of membrane types, with or Process Volume, mL 100 without prefilters, to fit any application need. Hold-up Volume, µL* <100 (membrane); <200 (membrane and prefilter) The filters are designed with a pressure- Maximum Inlet Pressure, bar (psi) 7 (100) resistant, domed housing and an optimized Maximum Operating Temperature, °C 45 *After air purge Luer-Lok® connection to ensure reliable delivery by automated systems.

Ordering Information Product Performance Description Pore Size (µm) Qty/Pk Catalogue No. Borosilicate glass fiber membrane (APFB) for clarifying aqueous and organic solutions containing high particle Analytical levels Millex®-PB Filter Unit 1.0 200 (8 x 25) SLPBDZ5NZ 1.0 1000 SLPBDZ5NK Low protein-binding Durapore® (PVDF) membrane for clarifying aqueous and mild organic solutions Millex®-HV Filter Unit 0.45 200 (8 x 25) SLHVDZ5NZ Prepare 1000 SLHVDZ5NK Low protein-binding Durapore® (PVDF) membrane and glass fiber prefilter for clarifying aqueous and mild organic solutions containing high particle levels Millex®-HV/PB Filter Unit 0.45 200 (8 x 25) SLHVBZ5NZ 1000 SLHVBZ5NK Nylon membrane for clarifying aqueous and organic solutions Millex®-GN Filter Unit 0.2 200 (8 x 25) SLGNDZ5NZ 1000 SLGNDZ5NK Millex®-HN Filter Unit 0.45 200 (8 x 25) SLHNDZ5NZ 1000 SLHNDZ5NK Nylon membrane and glass fiber prefilter for clarifying aqueous and organic solutions containing high particle levels Millex®-HN/PB Filter Unit 0.45 200 (8 x 25) SLHNBZ5NZ 1000 SLHNBZ5NK Low protein-binding hydrophilic LCR (PTFE) membrane for clarifying aqueous and organic solutions Millex®-LCR Filter Unit 0.2 200 (8 x 25) SLLGDZ5NZ 1000 SLLGDZ5NK 0.45 200 (8 x 25) SLCRDZ5NZ 1000 SLCRDZ5NK Low protein-binding hydrophilic LCR (PTFE) membrane and glass fiber prefilter for clarifying aqueous and organic solutions containing high particle levels Millex®-LCR/PB Filter Unit 0.45 200 (8 x 25) SLCRBZ5NZ 1000 SLCRBZ5NK

For more information visit: www.merckmillipore.com/NSmillex

162 www.merckmillipore.com Analytical Prepare 163 Qty/Pk Catalogue No. 501000501000 SLGNM25NS SLGNM25NK 200 (8 x 25) SLHNMZ5NZ* SLHNM25NS SLHNM25NK 501000501000 SLLGM25NS SLLGM25NK SLCRM25NS SLCRM25NK 501000200 (8 x 25) SLHVMZ5NZ* SLHVM25NS SLHVM25NK Pore Size Size Pore (µm) 0.20 0.45 0.20 0.45 0.45

for

than

precise

Sample tubes bursting

for delivery

resists

delivery throughput

reliable

or Automated

optimized

housing bulk greater

ensures

Testing,

either times

connection

Nylon membrane and graduated glass fiber prefilter for clarifying aqueous and mild organic solutions organic aqueous and mild for clarifying fiber prefilter glass and graduated membrane Nylon containing high particle levels *Automation-compatible Low protein binding hydrophilic (PTFE) membrane and glass fiber prefilter for clarifying aqueous and for clarifying fiber prefilter and glass (PTFE) membrane binding hydrophilic protein Low solutions containing high particle organic levels. Unit Filter Millex®-HPF/LG/LCR for clarifying fiber prefilter glass and graduated (PVDF) membrane binding Durapore® protein Low containing solutions high particle levels proteinaceous Millex®-HPF/HV Unit Filter Ordering Information Ordering Description Millex®-HPF/Nylon Unit Filter housing h automated filter-changing filter-changing h automated four

Dissolution inge filters without prefilters without filters inge

om automated filter-changing systems filter-changing om automated Available syr fr alignment and fit use wit systems eparation, Clarification of Solutions Clarification eparation, For more information visit: www.merckmillipore.com/NSmillex visit: For information more Containing High Particle Levels • Luer-Lok® Pr • Domed • Pressure-resistant • Applications Drug Features& Benefits • Two 3.9 100 250 7 (100) 45 HDPE Female Luer-Lok® Male Luer-Slip™ 2 Process Volume, mL Volume, Process µL Hold-up Volume, bar (psi) Pressure, Maximum Inlet °C Temperature, Maximum Operating Specifications Housing Material Fittings Inlet Fittings Outlet cm Area, Filtration www.merckmillipore.com Product Performance Product Filter particle-ladenFilter viscous samples or HPF Millex® include a filters Non-sterile remove to fiber prefilter glass graduated particles a 0.20 μm or 0.45 either and larger This fine filtration. for filter μm membrane provides combination of membranes than throughput greater significantly media, prefiltration without filters standard particle-laden when filtering especially in available solutions. HPF Millex® are filters and in filtration individual sample bulk for filter-changing automated use with tubes for a pressure- feature The filters systems. domed housing and an optimized resistant, reliable ensure connection to Luer-Lok® systems. delivery automated by Non-Sterile Millex® HPF Syringe Filters Millex® Syringe HPF Non-Sterile Samplicity® Filtration System Multi-sample vacuum filtration system The Samplicity® Filtration System provides a Features & Benefits convenient, high-throughput alternative to • Higher throughput with simultaneous syringe-tip filters when preparing samples for vacuum filtration of up to eight samples chromatography. The easy-to-use Samplicity® • Easy sample preparation of even highly system is the first vacuum-driven system with viscous or particulate-laden mixtures the designed-in flexibility to filter 1 to 8 • Ergonomic alternative to syringe filters samples directly into standard HPLC vials. Just • Millex Samplicity® membrane filters attach a vacuum pump, load samples with a provide long-trusted quality with low standard pipettor and flip the lever to recover extractables and low analyte binding particulate-free samples—even those with high viscosity or particulates—in seconds. In Applications addition, the system has a low hold-up Sample Filtration Prior to UHPLC, HPLC and volume, which allows processing of samples Mass Spec; Filtration of Hard-to-Filter as small as 300 µL. Samples

Product Performance Analytical Prepare

Specifications PTFE Durapore® PVDF Housing Material HDPE HDPE Housing Color White White Membrane Material Hydrophilic PTFE Hydrophilic Durapore® PVDF Pore Sizes 0.20 µm, 0.45 µm 0.45 µm Process Volume, mL 0.3–1.6 0.3–1.6 Hold-up Volume, µL <100 <100 Chemical Compatibility Few limitations Aqueous, some solvents Inlet Fitting Easy loading funnel Easy loading funnel Outlet Fitting Tip for smooth transfer to vial Tip for smooth transfer to vial

164 www.merckmillipore.com Analytical Prepare 165 µm

MAPHN0B50 128 x 85.5 x 14.6 0.65 Non-sterile 1050 MSPHN6B10 50 MSPHN6B50 MSPHNXB50 1050 MZPHN0W10 MZPHN0W50 50 2 2 2 2 Barex®/TiO Barex®/TiO acrylonitrile Styrene (SAN)/TiO Barex®/TiO Specifications Dimensions, cm Size Pore Sterility

and

your 0.5 0.1 Well Volume (mL) Volume Well Material Device Qty/Pk Catalogue No.

Substrates, solvent

filtrate

SAMPSYSGR SAMPSYSBL SAMPLG001 SAMPLCR01 SAMPLG004 SAMPLCR04 SAMPHV001 SAMPHV004 Messenger and

match

Peptide to

reduces

Second with

filtration

1 1 96 96 384 384 96 384 sample,

compatible Assays Assays,

and or better washing and washing or better

vacuum configurations

time

Kinase

Enzyme

ys adioactive wastes adioactive Automation collection f data consistent r application her • Superior • Multiple Ot Assa Applications Protein Features & Benefits • Saves • Pore Size (µm) Size Pore Qty/Pk Catalogue No. 0.45 0.20 0.45

Negatively Charged Phosphocellulose Charged Negatively 0.5 Negatively Charged Phosphocellulose Charged Negatively 0.5 mesh polyester mesh polyester Membrane Type Membrane and inositols. and inositols. 2+ phosphodiesterase

(NO), cGMP,

filter plates set the standard standard the set plates filter -PH Plate, 384 well-PH Plate, Phosphocellulose/ Charged Negatively -PH Plate, 96 well -PH Plate, 96 well PH Plate, + Hi Flow Phosphocellulose/ Charged Negatively plates with negatively charged membrane for enzyme assays for membrane charged negatively with plates HTS HTS HTS HTS Oxide HTS

cAMP,

for

Nitric

MultiScreen® MultiScreen® MultiScreen® Classic plates with negatively charged membrane for enzyme assays for membrane charged negatively with Classic plates MultiScreen® 96 well Plate, MultiScreen®-PH MultiScreen® Ordering Information Ordering Description MultiScreen® Samplicity® Filtration Bold Blue System, Millex PTFE Samplicity® Filters Hydrophilic 0.20 Millex PVDF Samplicity® Filters Hydrophilic 0.45 Description Samplicity® Glossy Green Filtration System, Ordering Information Ordering www.merckmillipore.com Enzyme assays are widely used in life science used in life widely are Enzyme assays Filter screening. and compound research facilitating of the separation by the plates, in enzymatic reactants the from products sensitive, highly enable reliable, reactions, assays. and automation-compatible MultiScreen® Save time and sample, increase consistency increase time and sample, Save For more information visit: www.merckmillipore.com/samplicity visit: For information more The filtration-based protocol produces produces protocol The filtration-based well- that are results reliable specific, in literature. referenced (PDE), for filtration-based enzyme assays. These enzyme assays. filtration-based for kinase, used for widely are systems versatile and endonuclease protease phosphatase, assays as second messenger as well assays, such MultiScreen® Filter Plates for Enzyme Assays Enzyme for Plates Filter MultiScreen® MultiScreen® Filter Plate Accessories For use with MultiScreen® Plates MultiScreen® column loaders let you combine MultiScreen® plate and distribute them into the cost savings of bulk media with the eight scintillation vials for detection. The convenience of 96-well filtration plates. All 96 patented punch tips remove each filter from wells are loaded simultaneously and its well separately, thereby eliminating the risk uniformly, eliminating the need for pipetting of cross-contamination and increasing slurries or using prepacked columns. reproducibility. MultiScreen® accessories for radiometric assay detection include the MultiScreen® Applications Punch Kit. After completing your multiwell 96-Well Chromatography, Radiometric radioactive assay, you can use the punch and Binding Assays disposable punch tips to simultaneously remove filters from eight wells of a

Ordering Information

Description Qty/Pk Catalogue No. Column Loaders for 96-Well Chromatoghraphy in Classic MultiScreen® Plates Centrifuge Alignment Frame 4 MACF09604 MultiScreen® Column Loader, 100 µL 1 MACL09600 MultiScreen® Column Loader, 25 µL 1 MACL09625 MultiScreen® Column Loader, 45 µL 1 MACL09645 MultiScreen® Column Loader, 80 µL 1 MACL09680

Analytical MultiScreen® Column Loader Scraper 3 MACL0SC03 Radioactive Assay Detection Accessories Disposable Punch Tips 5 x 10 MADP19650 MultiScreen® Multiple Punch 1 MAMP09608 Carrier Rack for 4 mL Vials 1 MACR08124 Prepare Carrier Rack for 7 mL Vials 1 MACR08127 Carrier Rack for 12 mm x 75 mm Tubes 1 MACR81275

MultiScreen®HTS Plate Carrier Slide (required for punching HTS Plates) 1 MSCP09600

Packard TopCount Adapter for MultiScreen®HTS 96-well Filter Plates 50 MSTPCWH50

166 www.merckmillipore.com Analytical Prepare 167

and

96 Non-sterile 1050 MSSLBPC10 10 MSSLBPC50 50 MSRLN0410 10 MSRLN0450 MDRLN0410 recovery

throughput

drug

binding

high

ving time and sample over the the over sample ving time and protocol

screening for

Assays

Device MaterialDevice Qty/Pk Catalogue No. Polypropylene 100 MSCPNPP00 PolystyrenePolypropylene 100 MSCPNPS00 40 MSCPNUV40 Styrene copolymer Polyolefin copolymer Polyolefin 96 Non-sterile 1.9 non-specific

m) efficiency sa shake-flask method shake-flask µ • 90-minute • Validated • Automation-compatible Applications Solubility Features& Benefits • Increased • Low ------Pore Size ( Size Pore - HTS 96 Non-sterile 128 x 85.5 x 14.60.5 128 x 85.5 x 40.7 128 x 85.5 x 40.7 Standard PlateStandard Solvinert Deepwell Collection Plates -PCF Filter Plates for Solubility for Plates Filter -PCF Assays MultiScreen® HTS

------Membrane Type Membrane Polycarbonate 0.4 Hydrophilic PTFEHydrophilic 0.45 PTFEHydrophilic 0.45 Plate

properties.

-PCF HTS ADME

Collection

its

Number of Wells Number Sterility MultiScreen® Plates MultiScreen® 96-well MultiScreen® UV MultiScreen® 96-well Analysis Polypropylene 96-well Collection Plate V-bottom Required Equipment Required Polystyrene 96-well Collection Plate MultiScreen® Ordering Information Ordering Description Well Volume, mL Volume, Well Dimensions, cm Specifications Filter plate Filter MultiScreen® Solvinert MultiScreen® Plate Deep Well MultiScreen® Solvinert Plate For more information visit: www.merckmillipore.com/cellculture visit: For information more PCF filter plates have made early-stage made early-stage have plates PCF filter to solubility possible. Compared screening plate filter methods, shake-flask traditional are amounts of compounds, use low methods are and fast. Results automatable, reliable, with and correlate reproducible highly other values. Unlike published literature method this methods, high-throughput in solution. compounds measures www.merckmillipore.com Increased screening throughput and efficiency throughput screening Increased solubility water is an important Determining discovery and drug in the step early precipitates Insoluble process. development potentially in bioassays, cause false positives solubility Water valuable resources. wasting and can help absorption also influences drug predict MultiScreen® MultiScreen® Filter Plates with Durapore® Membrane High-throughput filtration with low protein binding Durapore® PVDF membranes provide high Applications flow rates and throughput, low levels of Receptor/Ligand Binding Assays, Protein extractables and broad chemical compatibility. Kinase/Phosphatase Precipitation Assays, Hydrophilic Durapore® membranes bind far Bead-Based Assays less protein than nylon, nitrocellulose or PTFE membranes.

Features & Benefits • Multiple configurations and applications • Chemical compatibility • Automation-compatible • Superior vacuum filtration and filtrate collection

Ordering Information Membrane Type/Pore Size Number of Wells Well Volume (mL) Device Material Sterility Qty/Pk Catalogue No. MultiScreen® Filter Plates with Durapore® Membrane Durapore® PVDF, 1.2 µm 96 0.05 - 0.25 Styrene Non-sterile 10 MSBVN1210 Styrene Non-sterile 50 MSBVN1250

Barex®/TiO2 Non-sterile 50 MSBVN1B50 Styrene Sterile 10 MSBVS1210 Durapore® PVDF, 0.65 µm 96 0.05 - 0.25 Styrene Non-sterile 10 MSDVN6510 Analytical Styrene Non-sterile 50 MSDVN6550

Barex®/TiO2 Non-sterile 50 MSDVN6B50 Durapore® PVDF, 0.22 µm 96 0.05 - 0.25 Styrene Non-sterile 10 MSGVN2210 Styrene Non-sterile 50 MSGVN2250 Prepare

Barex®/TiO2 Non-sterile 50 MSGVN2B50 Styrene Sterile 10 MSGVS2210 Durapore® PVDF, 0.45 µm 96 0.05 - 0.25 Styrene Non-sterile 10 MSHVN4510 Styrene Non-sterile 50 MSHVN4550

Barex®/TiO2 Non-sterile 50 MSHVN4B50 Styrene Non-sterile 10 MSHVS4510 384 0.02 - 0.1 Styrene Non-sterile 10 MZHVN0W10 Styrene Non-sterile 50 MZHVN0W50 96 <2.0 Polypropylene Non-sterile 25 MVHVN4525

168 www.merckmillipore.com Analytical Prepare 169 MAHFB1H60 MAHFC1H60 MAFBN0B50 MAFCN0B50 50 MZFBN0W50 50 MSFCN6B50 50 MZFCN0W50 50 MSFBN6B50 Non-sterile 10Non-sterile MZFBN0W10 10Non-sterile MSFCN6B10 50Non-sterile 10 MSFCNXB50 MZFCN0W10 Non-sterile 60 Non-sterile 60 Non-sterile 50 Non-sterile 50 Non-sterile 10Non-sterile MSFBN6B10 50 MSFBNXB50 2 2 2 2 2 2 2 2 2 2 Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Barex®/TiO Polypropylene Non-sterile 25 MVFCN1225

filtrate

Protein Sample

applications of

96 96 96 96 96 384 96 384 96 96 96 Number of WellsNumber Material Device Sterility Qty/Pk Catalogue No.

and

Assays,

PVDF PVDF PVDF PVDF

filtration

Precipitation Binding

mesh mesh mesh mesh

TCA Durapore® Durapore® Durapore® Durapore® compatibility

vacuum configurations

µm µm µm µm

collection eparation, eceptor/Ligand µm µm/0.65 µm/polyester µm/polyester µm µm/0.65 µm µm/0.65 µm/polyester µm/0.65 µm/polyester

• Chemical Pr Applications R Features& Benefits • Multiple • Automation-compatible • Superior 1.2 1.2 1.2 1.0 1.0 1.0 1.2 1.2 1.0 1.0 1.2

FC FC FC FB FB FB FC FB FB FC FC

fiber fiber fiber fiber fiber fiber fiber fiber fiber fiber fiber

Glass Glass Glass Membrane Type/Pore Size Type/Pore Membrane Glass Glass Glass Glass Glass Glass Opaque Glass Opaque Glass

C B

Fiber Fiber

FC Plate FB Plate FB Plate FC Plate + Hi Flow FC Plate + Hi Flow + Hi Flow FB Plate + Hi Flow Filter Plates with Glass Fiber Prefilter Glass Fiber with Plates Filter Glass Glass

HTS HTS HTS HTS HTS HTS

HTS MultiScreen® MultiScreen® MultiScreen® Harvest FC MultiScreen® Plate, MultiScreen® MultiScreen® MultiScreen® MultiScreen® High Volume with Glass Fiber with High Volume MultiScreen® with High Volume MultiScreen® Glass Fiber C MultiScreen® Classic 96-well Plates with Glass Fiber Prefilter Glass Fiber with Plates Classic 96-well MultiScreen® MultiScreen® Harvest Plates - MultiScreen® Plates with Glass Fiber for Traditional Cell Harvesting Assays Traditional for Glass Fiber Harvest with Plates - MultiScreen® Plates Harvest FB MultiScreen® Plate, MultiScreen® Ordering Information Ordering Description MultiScreen® www.merckmillipore.com High-throughput clarification and prefiltration and clarification High-throughput have resin binder with filters Glass fiber excellent and are strength wet superior analysisand prefiltration, qualitative for or contaminated heavily for especially also are They particulate-laden samples. of aqueous clarification used for widely solutions. MultiScreen® Filter Plates with Glass Fiber Filters Fiber Glass with Plates Filter MultiScreen® Membrane Selection Guide Microporous Membranes Organized by Application Durapore® Durapore® PVDF MF-Millipore™ MCE Millipore PLUS Express® PES Isopore™ Polycarbonate Fluoropore™ PTFE Omnipore™ PTFE Mitex™ PTFE PTFE LCR MCE V-Series Nylon Silver PVC Lab Applications Clarification of cell lysates ● ● and tissue homogenates Cell cytology ● ● Sterilizing liquid filtration ● ● ● Air sterilization ● Mycoplasma reduction ● ● Solvent filtration ● ● ● ● ● ● Tissue culture media filtration ● ● Microdialysis of DNA and ● proteins Fluorescent bacteriological ● assays General filtration and clarification of aqueous ● ● ● ● solutions Clarifying acids and bases ● ● ● ● ● ●

Analytical Chemotaxis ● SEM analysis ● Epifluorescence microscopy ● Venting applications ● ● HPLC solvent filtration ● ● ●

Prepare Environmental Monitoring Applications Alpha particle monitoring ● Air monitoring ● ● ● ● ● ● Industrial particle monitoring ● ● ● ● Particle collection and ● analysis Gravimetric analysis ● ●

NOTE: This chart provides general recommendations. Contact your local Merck Millipore technical service representative to discuss the requirements of your specific application: www.merckmillipore.com/techservice

170 www.merckmillipore.com Membrane Selection Guide Microporous Membranes Organized by Performance

Typical Flow Rate0 Surface Water Air Min. Ave. Bubble Pt. Refractive 4 2 1 2 2 3 Membrane Type Chemistry Membrane Code Pore size (µm) (mL/min/cm /psi) (L/min/cm /psi) (Air with H2O, psi) Index MF-Millipore™ Hydrophilic VSWP 0.025 0.0 0.0 306.0 1.50 MCE VMWP 0.05 0.1 0.0 255.3 1.50 VCWP 0.1 0.1 0.1 204.5 1.50 GSWP 0.22 1.4 0.3 51.1 1.51 PHWP 0.3 2.4 0.5 34.8 1.51 HAWP 0.45 4.5 0.8 31.9 1.51 DAWP 0.65 10.6 2.3 17.0 1.51 AAWP 0.8 14.3 2.7 14.5 1.51 RAWP 1.2 20.4 3.5 11.0 1.52 SSWP 3 24.1 4.9 10.2 1.50 SMWP 5 43.7 6.4 6.1 1.50 SCWP 8 46.8 6.9 6.1 1.52 Millipore Express® Hydrophilic GPWP 0.22 1.5 0.5 20.2 NA PLUS PES HPWP 0.45 2.3 1.1 10.0 NA Durapore® PVDF Hydrophilic VVLP 0.1 ≥ 0.33 0.1 72.5 1.42 GVHP 0.22 ≥ 1 0.2 50.0 1.42 HVLP 0.45 ≥ 2.6 0.9 22.5 1.42 DVPP 0.65 ≥ 6 1.1 16.0 1.42 SVLP 5 ≥ 15.4 1.6 2.9 1.42 Isopore™ Hydrophilic VCTP 0.1 0.2 0.1 101.0 1.60 polycarbonate GTTP 0.2 1.0 0.3 58.0 1.60 Analytical GTBP 0.2 0.7 0.3 75.4 1.60 HTBP 0.4 1.2 0.4 42.1 1.60 DTTP 0.6 2.3 0.8 17.0 1.60 ATTP 0.8 4.1 1.4 13.0 1.60 RTTP 1.2 6.2 2.3 10.0 1.60

TTTP 2 8.5 2.0 5.0 1.60 Prepare TSTP 3 29.0 4.9 3.0 1.60 TMTP 5 39.4 5.4 1.9 1.60 TETP 8 44.0 5.5 1.3 1.60 TCTP 10 114.7 7.2 1.0 1.60 Nylon Hydrophilic GNWP 0.2 0.6 0.2 42.1 NA HNWP 0.45 1.1 0.3 30.0 NA ANWP 0.8 1.4 0.3 8.0 NA RNWP 1.2 1.6 0.5 5.9 NA LCR PTFE Hydrophilic FHLC 0.45 1.6 0.6 11.0 NA Omnipore™ PTFE Hydrophilic JVWP 0.1 0.1 0.4 342.3 NA JGWP 0.2 0.3 0.7 197.3 NA JHWP 0.45 1.1 1.2 114.6 NA JAWP 1 3.4 3.4 52.2 NA JMWP 5 11.4 5.3 30.5 NA JCWP 10 22.7 6.8 10.2 NA Fluoropore™ PTFE Hydrophobic FGLP 0.22 0.9 0.4 14.5 NA FHLP 0.45 1.3 1.6 9.1 NA FHUP 0.45 2.0 1.1 9.1 NA FALP 1 4.8 2.7 7.3 NA FSLW 3 13.8 6.4 1.5 NA Mitex™ PTFE Hydrophobic LSWP 5 14.1 2.0 0.7 NA LCWP 10 32.3 5.0 0.4 NA Durapore® PVDF Hydrophobic VVHP 0.1 ≥ 0.33 0.1 26.1 1.42 GVHP 0.22 ≥ 1 0.2 18.0 1.42 HVHP 0.45 ≥ 2.6 0.5 8.7 1.42

0 NOTE: Flow rates listed are based on measurements with clean water and air, and represent typical values. Values presented here do not establish specifications. 1 Water flow rates were measured through 9.6 cm2 of membrane, using 500 mL of water at 25 °C and 27.5 in. Hg of vacuum. Hydrophobic membranes were wet with solvents and then exchanged in water for testing. 2 Air flow rates were measured on a 25 mm disc at 10 psi using a mass flow meter. 3 Membranes were tested with water, except for Mitex™, Fluoropore™ and hydrophobic Durapore® membranes, which were tested with methanol. 4 Corresponds to the first four letters of the catalogue number. www.merckmillipore.com 171 MF-Millipore™ Membrane Filters Mixed cellulose esters Biologically inert mixtures of cellulose acetate Features & Benefits and cellulose nitrate have made • Versatile filter for biological and MF-Millipore™ membrane filters one of the environmental monitoring applications most widely used membranes in analytical • Available in a range of pore sizes, colored and research applications. black or white, with or without a gridded surface MF-Millipore™ filters without Triton® • Compatible with ethylene oxide, gamma surfactant contain minimum amounts of irradiation, and autoclave sterilization wetting agent and have a lower water methods extractable content than standard MF-Millipore™ filters.

Specifications Color White or black Surface Plain or gridded Wettability Hydrophilic Sterilization Ethylene oxide, autoclavable (121 oC at 1 bar) Operating Temperature 55 oC maximum Protein Binding 150 µg/cm2 Bacterial Endotoxins 8.0 EU/mL Gravimetric Extractables <1.0%

Detailed Specifications Analytical Applications Filter Code1 Color Pore Size Bubble Point2 Thickness Water Flow Rate3 Typical Air Flow4 Porosity (µm) (psi) (µm) (mL/min/cm2) (L/min/cm2) (%) Standard MF-Millipore™ Membranes Microdialysis of DNA and proteins VSWP White 0.025 306 105 - >0.12 70 VMWP White 0.05 255 105 - >0.26 72

Prepare VCWP White 0.1 205 105 - >0.37 74 Sterilizing filtration, bioassays GSWP White 0.22 51.1 150 >20.1 2.7 75 Sterilizing filtration, air monitoring, particle PHWP White 0.3 35.7 150 >34.0 5.1 77 monitoring, particle removal, bioassays Clarification of aqueous solutions, particle HAW* White 0.45 30.6 150 >48.1 8.3 79 removal and analysis, microbiology analysis Fluorescent bacteriological assays, particle HAB** Black 0.45 33.6 150 >69.4 - 79 monitoring, bioassays Particle monitoring, particle removal, dairy DAWP White 0.65 17.0 150 >140 23.4 81 microbiology, retention of yeasts, molds and algae Air monitoring, particle monitoring, particle AAW** White 0.8 14.0 150 >184 26.7 82 removal, bioassays Fluorescent assays, particle monitoring, AAB** Black 0.8 16.2 150 >195 - 82 air monitoring Clarification of aqueous solutions RAWP White 1.2 11.2 150 >284 35.2 82 QC of fluid holding tanks, fluid monitoring, SSWP White 3 10.2 150 >347 49.3 83 air monitoring, particle collection and analysis QC of fluid holding tanks, fluid monitoring, SMWP White 5 8.0 135 >520 64.5 84 particle collection and analysis QC of fluid holding tanks, fluid monitoring, SCWP White 8 6.1 135 >625 68.9 84 air monitoring, particle collection and analysis *Available with plain (P) or gridded (G) surface. 1Corresponds to first 4 digits of catalogue number. 2Bubble point tested with water. 3Water Flow Rate measured with 47 mm disc and 500 mL of water at 25 oC and 27.5 inHg vacuum. 4Air flow values measured at 10 psi with a digital flow meter. Values represent typical performance and are not established specifications.

172 www.merckmillipore.com Analytical Prepare 4 2 1 1 1 3 173 HAWG04700 AAWP037P0 AAWP037PM AAWP04700 AAWP0470M AAWP09000 AAWP14250 RAWP01300 RAWP02500 RAWP03700 RAWP04700 RAWP09025 RAWP14250 HAWG01300 HAWG02500 HAWG03700 AAWP01300 AAWP02500 AAWP03700 10025 SMWP04700 50100 SMWP09025 100 SMWP14250 SCWP01300 100 SCWP0190R 100 SCWP02500 25 SCWP04700 50 SCWP09025 SCWP14250 100 100 50 pr 100 50 pr 100 50 100 100 100 100 25 50 100100 SSWP01300 100 SSWP02500 25 SSWP04700 50100 SSWP09025 100 SSWP14250 SMWP01300 100 SMWP02500 SMWP03700 100 100 100 100 100 100 47 90 142 13 19 x 42 25 47 90 142 47 37 37 47 47 90 142 13 25 37 47 90 142 13 25 47 90 142 13 25 37 13 25 37 Filter Diameter (mm) Diameter Filter 13 Qty/Pk25 Catalogue No. 37 8.0 1.2 3.0 5.0 Standard MF-Millipore™ Membranes, white, gridded MF-Millipore™ white, Membranes, Standard 0.45 Pore Size (µm) Size Pore 0.8 Monitor refills with thick absorbent pads for liquid monitoring. for liquid pads absorbent thick with refills Monitor monitoring. for liquid pads absorbent thick weight pairs) with (matched refills Monitor by fibers. contamination avoid to stock roll and controlled selected specifically Cut from Monitor refills with thin absorbent pads for aerosol monitoring. for aerosol pads thin absorbent with refills Monitor pairs. filter weight Matched For asbestos monitoring applications. monitoring For asbestos 3 4 5 2 1 2 1 1 HAWP05000 HAWP09000 HAWP14250 DAWP01300 DAWP02500 DAWP04700 DAWP09025 DAWP14250 HAWP01300 HAWP02400 HAWP02500 HAWP03700 HAWP04700 HAWP0470M 100 100 50 100 100 100 25 50 100100 GSWP04700 50 GSWP09000 100100 GSWP14250 PHWP02500 25 PHWP04700 50100 PHWP09025 100 PHWP14250 100 100 100 50 pr 100100 VSWP01300 100 VSWP02500 25 VSWP04700 50100 VSWP09025 100 VSWP14250 VMWP01300 100 VMWP02500 25 VMWP04700 100100 VMWP09025 VCWP01300 100 VCWP02500 25 VCWP04700 50100 VCWP09025 100 VCWP14250 GSWP01300 100 GSWP02500 GSWP03700 50 90 142 13 25 47 90 142 47 47 90 142 25 47 90 142 13 24 25 37 47 25 47 90 142 13 25 47 90 13 25 47 90 142 13 25 37 Filter Diameter (mm) Diameter Filter Qty/Pk13 Catalogue No. 0.65 0.3 0.45 0.05 0.1 0.22 0.025 Pore Size (µm) Size Pore plain MF-Millipore™ white, Membranes, Standard Ordering Information Ordering www.merckmillipore.com Ordering Information - Continued Pore Size (µm) Filter Diameter (mm) Qty/Pk Catalogue No. Pore Size (µm) Filter Diameter (mm) Qty/Pk Catalogue No. 0.8 13 100 AAWG01300 Standard MF-Millipore™ Membranes, black, gridded 25 100 AAWG0250C5 0.45 13 100 HABG01300 37 100 AAWG037001 25 100 HABG02500 47 100 AAWG04700 47 100 HABG04700 1.2 25 100 RAWG02500 0.8 13 100 AABG01300 25 100 RAWG0250C5 25 100 AABG02500 47 100 RAWG04700 37 100 AABG037001 Standard MF-Millipore™ Membranes, black, plain 47 100 AABG04700 0.45 25 100 HABP02500 1Monitor refills with thin absorbent pads for aerosol monitoring. 2 47 100 HABP04700 Matched weight filter pairs. 3Monitor refills with thick absorbent pads for liquid monitoring. 0.8 25 100 AABP02500 4Monitor refills (matched weight pairs) with thick absorbent pads for liquid monitoring. 47 100 AABP04700 5Cut from specifically selected and controlled roll stock to avoid contamination by fibers. For asbestos monitoring applications.

Detailed Specifications Applications Filter Code1 Color Pore Size Bubble Point2 Thickness Water Flow Rate3 Typical Air Flow4 Porosity (µm) (psi) (µm) (mL/min/cm2) (L/min/cm2) (%) MF-Millipore™ Membranes without Triton® surfactant For biological solutions, cell contact, GSTF White 0.22 51.1 150 >20 2.7 77 or very small volumes, requiring HATF White 0.45 30.6 150 >48 8.3 79 surfactant-free surfaces RATF White 1.2 11.2 150 >284 35.2 82 Analytical 1Corresponds to first 4 digits of catalogue number. 2Bubble point tested with water. 3Water Flow Rate measured with 47 mm disc and 500 mL of water at 25 oC and 27.5 inHg vacuum. 4Air flow measured at 10 psi. Values represent typical performance and are not established specifications. Prepare

Ordering Information Pore Size (µm) Filter Diameter (mm) Qty/Pk Catalogue No. MF-Millipore™ Membranes without Triton® surfactant, white, plain 0.22 13 100 GSTF01300 25 100 GSTF02500 47 100 GSTF04700 90 100 GSTF09000 142 50 GSTF14250 0.45 13 100 HATF01300 25 100 HATF02500 47 100 HATF04700 90 25 HATF09025 142 50 HATF14250 1.2 47 100 RATF04700 142 50 RATF14250

Accessory Description Qty/Pk Catalogue No. Filter Forceps, blunt end, stainless steel 3 XX6200006P

For more information visit: www.merckmillipore/filterdiscs

174 www.merckmillipore.com Analytical Prepare 175 ) 2 150 150 150 4 4 4 4 4 Protein Binding Protein (µg/cm

3 4.9 1.7 0.9 - - - - - Typical Air Flow Typical (L/min/cm²) 2 (mL/min/cm²) Water Flow Rate Flow Water

low

very

C at 1 bar) (both o

have

sizes

specifications. filters

pore

≥9 psi, air with methanol≥9 psi, air with - ≥18 psi, air with methanol≥18 psi, air with - ≥26 psi, air with methanol≥26 psi, air with - established

several

membrane

not

are

Hydrophobic Hydrophobic Hydrophobic Wettability (psi) Bubble Point and

otein binding to minimize interaction interaction minimize binding to otein ydrophilic and hydrophobic varieties) to to varieties) and hydrophobic ydrophilic h needs application suit your pr with your sample and maximize recovery maximize and sample your with • Durapore® Features& Benefits • Available Pore Size Size Pore (µm) performance

C and 27.5 inHg vacuum through 47 mm disc. C and 27.5 inHg vacuum through o 1 typical

µm C maximum

o 85 White Plain or hydrophobic Hydrophilic (121 autoclavable irradiation, gamma oxide, Ethylene 125 0.5 EU/mL <0.5% HVHP 0.45 GVHP 0.22 VVHP 0.1 SVLP 5.0 Hydrophilic water ≥3 psi, air with >208 DVPP 0.65 Hydrophilic water ≥15 psi, air with >78 HVLP 0.45 Hydrophilic water ≥22 psi, air with >34 GVWP 0.22 Hydrophilic water ≥50 psi, air with >12 VVLP 0.1 Hydrophilic water ≥75 psi, air with >4 Filter Code Filter represent

Values

psi.

measured

rate

flow

Air Corresponds to first 4 digits of catalogue first number. to Corresponds at 25 water 500 mL of with measured Rate Flow Water Operating Temperature Operating Surface Wettability Sterilization Specifications Color Gravimetric Extractables Gravimetric Thickness Endotoxins Bacterial Air clarification, gas filtration, gas filtration, Air clarification, filtration solvent Air sterilization, gas sterilization, sterilization, gas Air sterilization, filtration solvent Air sterilization, gas sterilization gas Air sterilization, Clarifying filtration of biological filtration Clarifying solutions, particle monitoring Clarifying filtration of biological filtration Clarifying solutions Clarifying filtration of biological filtration Clarifying solutions Sterilizing filtration of biological filtration Sterilizing solutions 3 1 2 Detailed Specifications Detailed Applications in reduction Mycoplasma biological solutions www.merckmillipore.com Product Performance Product Polyvinylidene fluoride (PVDF) fluoride Polyvinylidene high flow provide membranes Durapore® extractables and low and throughput, rates Hydrophlic compatibility. chemical broad binds far membrane less protein Durapore® or PTFE nitrocellulose, nylon, than membranes. Durapore® Membrane Filters Membrane Durapore® Ordering Information

Pore Size (µm) Filter Diameter (mm) Qty/Pk Catalogue No. Pore Size (µm) Filter Diameter (mm) Qty/Pk Catalogue No. Hydrophilic Durapore® Membrane Filters 5.0 13 100 SVLP01300 0.1 13 100 VVLP01300 5.0 13 100 SVLP01300 0.1 25 100 VVLP02500 5.0 25 100 SVLP02500 0.1 47 100 VVLP04700 5.0 47 100 SVLP04700 0.1 90 50 VVLP09050 5.0 75 50 SVLP07550 0.1 142 50 VVLP14250 5.0 47 100 SVWG04700 0.22 13 100 GVWP01300 5.0 90 50 SVLP09050 0.22 25 100 GVWP02500 Hydrophobic Durapore® Membrane Filters 0.22 47 100 GVWP04700 0.1 47 100 VVHP04700 0.22 90 50 GVWP09050 0.22 13 100 GVHP01300 0.22 100 50 GVWP10050 0.22 25 100 GVHP02500 0.22 142 50 GVWP14250 0.22 47 100 GVHP04700 0.45 13 100 HVLP01300 0.22 90 50 GVHP09050 0.45 25 100 HVLP02500 0.22 142 50 GVHP14250 0.45 47 100 HVLP04700 0.22 304 mm x 3 m 1 GVHP00010 0.45 90 50 HVLP09050 0.45 13 100 HVHP01300 0.45 304 mm x 3 m 1 HVLP00010 0.45 25 100 HVHP02500 0.65 13 100 DVPP01300 0.45 47 100 HVHP04700 0.65 25 100 DVPP02500 0.45 90 50 HVHP09050 0.65 47 100 DVPP04700 0.45 142 50 HVHP14250 Analytical 0.65 82 50 DVPP08250 Accessory 0.65 90 50 DVPP09050 Filter Forceps, blunt end, stainless steel 3 XX6200006P 0.65 142 50 DVPP14250 *The membrane disc (cat. no. SVWG04700) has a gridded surface. 0.65 293 25 DVPP29325 Prepare

For more information visit: www.merckmillipore/filterdiscs

176 www.merckmillipore.com Analytical Prepare 3 177 ) 2 Water Flow Rate Flow Water (mL/min/cm C and 27.5 in. Hg vacuum o 170140 >27 >44 Thickness (µm) 2 Bubble Point (psi) Applications Filtration, Buffer Filtration, Tissue Sterile Media Filtration Culture

Pore Size Pore (µm) 0.22 20 0.45 10 1 ethylene

throughput

Corresponds to first 4 digits of catalogue number. to Corresponds alcohol. isopropyl with Bubble point measured at 25 water 500 mL of with measured rate flow Water high through 47 mm disc. through Detailed Specifications Detailed Code Filter GPWP 1 2 3 HPWP

100100 HPWP01300 100 HPWP02500 50 HPWP04700 50 HPWP09050 HPWP14250 100100 GPWP01300 100 GPWP02500 50 GPWP04700 50 GPWP09050 GPWP14250 3 XX6200006P autoclave, and

filtration

sterilized

fast

be ten process time process ten

xide, or gamma irradiation for sterile sterile for irradiation xide, or gamma filtration applications filtration shor o 13 25 47 90 142 13 25 47 90 142 • Can Features& Benefits • Ultra 0.45 Pore Size (µm) Size Pore (mm) Diameter Filter Qty/Pk Catalogue No. C at 1 bar) o white plain 0.5 EU/mL <0.5% autoclave irradiation, gamma oxide, Ethylene (121 Surface Endotoxins Bacterial Extractables Gravimetric Sterilization Specifications Color Filter Forceps, blunt end, stainless Forceps, Filter steel Accessory Millipore Express® PLUS Membrane PLUS Express® Millipore 0.22 Ordering Information Ordering Description For more information visit: www.merckmillipore/filterdiscs visit: For information more www.merckmillipore.com Product Performance Product Polyethersulfone (PES) Polyethersulfone membrane PLUS Express® The Millipore of tissue ultrafast filtration provides buffers media, additives, culture and other high throughput, solutions. This aqueous is also membrane protein-binding low sterile of our ready-to-use used in many devices. filtration Millipore Express® PLUS Membrane Filters Membrane PLUS Express® Millipore Fluoropore™ and Omnipore™ PTFE Membrane Filters PTFE with and without backing material Features & Benefits Applications • Biologically and chemically inert Fluoropore™ Membrane: Clarifying Acids, • High porosity yields high flow rates Bases and Solvents; Air Monitoring; Filtering • Hydrophobic and hydrophilic PTFE and Venting Gases; UV Spectroscopy varieties available for filtration of aqueous and organic-based samples Omnipore™ Membrane: Filtration of Aqueous Solutions, Clarifying Acids and Alkaline Solution

Product Performance

Fluoropore™ Membrane is a hydrophobic, polytetrafluoroethylene (PTFE) polymer membrane bonded to a high density polyethylene support. (Catalogue numbers containing “”FHUP”” have no backing.) Fluoropore™ Membranes provide broad chemical compatibility, high flow rates and Analytical consistency.

Omnipore™ Membrane is hydrophilic PTFE Specifications compatible with virually all solvents, acids, Sterilization Ethylene oxide or autoclave (121 oC at 1 bar)

Prepare and alkaline solutions. Bacterial Endotoxins 0.5 EU/mL Gravimetric Extractables <0.5%

Detailed Specifications Applications Filter Code1 Pore Size Bubble Point2 Thickness Liquid Flow Rate3 Air Flow Rate4 Oper. Temp. Porosity (µm) (psi) (µm) (mL/min/cm2) (L/min/cm2) (°C) (%) Fluoropore™ Membranes (hydrophobic) Clarifying acids, bases, and FGLP 0.2 14.8 150 24 5 130 85 solvents, air monitoring, filtering FHLP 0.45 9.2 150 60 9 130 85 or venting gases, UV spectroscopy FALP 1.0 7.0 150 110 16 130 85 FSLW 3.0 1.0 150 286 20 130 85 FHUP 0.45 6.2 50 75 9 130 NA Omnipore™ Membranes (hydrophilic) Clarifying acids, alkaline solutions, JVWP 0.1 23.6 30 >10 and virtually all solvents JGWP 0.2 13.6 65 >28 JHWP 0.45 7.9 65 >74 JAWP 1.0 3.6 85 >156 JMWP 5 2.1 85 >391 JCWP 10 0.7 85 >446 1Corresponds to first 4 digits of catalogue number. 2Bubble point determined with methanol, except FHUP which was tested with isopropyl alcohol (IPA). Omnipore™ membrane tested with IPA. 3Fluoropore™ membrane tested with methanol at 27.5 in. Hg. (average values). For Omnipore™ membrane, 100 mL water, 20 °C, 47 mm disc, 8.97” in. Hg. vacuum 4Air flow rates for Fluoropore™ membrane are tested at 10 psi.

178 www.merckmillipore.com Analytical Prepare 179 JAWP14225 JAWP09025 JAWP04700 JAWP02500 JAWP01300 25 JCWP14225 25 JCWP09025 100 JCWP04700 100 JCWP02500 100 JCWP01300 25 JMWP14225 25 JMWP09025 100 JMWP04700 100 JMWP02500 100 JMWP01300 25 25 100 100 100 25 JHWP14225 25 JHWP09025 100 JHWP04700 100 JHWP02500 100 JHWP01300 25 JGWP14225 25 JGWP09025 100 JGWP04700 100 JGWP02500 100 JGWP01300 25 JVWP14225 25 JVWP09025 100 JVWP04700 100 JVWP02500 100 JVWP01300 142 90 47 25 13 142 90 47 25 13 142 90 47 25 13 142 90 47 25 13 142 90 47 25 13 142 90 47 25 13 10.0 5.0 1.0 0.45 0.2 Omnipore™ (hydrophilic PTFE) Membrane Filters PTFE) Membrane (hydrophilic Omnipore™ Pore Size (µm) Size Pore (mm) Diameter Filter 0.1 Qty/Pk Catalogue No. FALP14250 FALP09050 FALP04700 FALP03700 FALP02500 FALP01300 10 FSLW14200 25 FSLW09025 100 FSLW04700 100 FSLW03700 100 FSLW02500 50 50 100 100 100 100 50 FHLP14250 50 FHLP09050 100 FHLP04700 100 FHLP03700 100 FHLP02500 100 FHLP01300 50 FGLP14250 50 FGLP09050 100 FGLP04700 100 FGLP02500 100 FHUP04700 100 FGLP01300 142 90 47 37 25 142 90 47 37 25 13 142 90 47 37 25 13 142 90 47 25 47 13 3.0 1.0 0.45 Fluoropore™ (PTFE) Membrane Filters (PTFE) Membrane Fluoropore™ Unlaminated Fluoropore™ (PTFE) Membrane Filter (PTFE) Membrane Fluoropore™ Unlaminated 0.45 0.22 Ordering Information Ordering (µm) Size Pore (mm) Diameter Filter Qty/Pk Catalogue No. www.merckmillipore.com For more information visit: www.merckmillipore/filterdiscs visit: For information more Mitex™ PTFE Membrane Filters Pure PTFE Mitex™ PTFE Membrane is unaffected by Features & Benefits almost all liquids, including organic solvents, • Broad chemical compatibility concentrated acids and bases, propellants, • Biologically and chemically inert and cryogenic fluids. Mitex™ membrane is • Stable at temperatures in excess of 260 °C unbacked, yet is easy to handle, combining (500 °F) and below -100 °C (-148 °F) the convenience of a backed membrane with the versatility of a pure PTFE membrane. Applications Mitex™ membrane is hydrophobic. Clarifying Acids, Bases, and Cryogenic Fluids; Clarifying Propellants; Analyzing Hydraulic Fluids; Isolating RNA

Specifications Sterilization Ethylene oxide or autoclave (121 oC at 1 bar) Bacterial Endotoxins 0.5 EU/mL Gravimetric Extractables <0.5%

Detailed Specifications Filter Code1 Pore Size Bubble Point2 Thickness Water Flow Rate3 Air Flow Rate4 Oper. Temp. (µm) (psi) (µm) (mL/min/cm2) (mL/min/cm2) (°C) LSWP 5 0.9 170 >47 >117 260 LCWP 10 0.7 130 >125 >167 260 Analytical 1Corresponds to first 4 digits of catalogue number. 2Bubble point measured with ethanol at 23 oC. 3Water Flow Rate measured with 500 mL of water at 25 oC and 27.5 in. Hg vacuum through 47 mm disc. 4Air flow rates measured on 47 mm disc at 10 mbar with digital flow meter. Prepare

Ordering Information

Description Pore Size (µm) Filter Diameter (mm) Qty/Pk Catalogue No. Mitex™ PTFE Membrane Filters, plain 5.0 13 100 LSWP01300 25 100 LSWP02500 37 100 LSWP03700 47 100 LSWP04700 90 25 LSWP09025 142 50 LSWP14250 10.0 13 100 LCWP01300 25 100 LCWP02500 47 100 LCWP04700 90 25 LCWP09025 142 50 LCWP14250 Mitex™ PTFE Membrane Filters, gridded 5.0 25 100 LSWG02500 47 100 LSWG04700 10.0 25 100 LCWG02500 47 100 LCWG04700

For more information visit: www.merckmillipore/filterdiscs

180 www.merckmillipore.com Analytical Prepare 181

Porosity (%) filter

used add

Oper. Temp. Oper. (°C) 130 80 be

not 4

can 100100 FHLC01300 100 FHLC02500 FHLC04700

will

) 2 membrane

membrane

Typical Air Flow Rate Air Flow Typical (L/min/cm 1.1 3 ueous fluids without prior wetting prior without ueous fluids ) 2 xtractables to your samples or solvents samples your xtractables to 13 25 47 aq e • Ultraclean Features& Benefits • Hydrophilic Applications Phase Filtration; Mobile Clarifying HPLC Solutions; Protein Bases and Dilute Acids, Isolating RNA C and 27.5 in. Hg vacuum through 47 mm disc. C and 27.5 in. Hg vacuum through o (mL/min/cm Water Flow Rate Flow Water 0.45 Pore Size (µm) Size Pore (mm) Diameter Filter Qty/Pk Catalogue No. Thickness (µm) number.

(psi) 2 catalog

Bubble Point digits

first

Pore Size Pore (µm) 0.45 9.2 140 28.4 1 Corresponds in methanol. Tested at 25 water 500 mL of with measured Rate Flow Water at 10 psi. Measured Description Filters PTFE Membrane LCR Ordering Information Ordering 1 2 3 4 Filter Filter Code FHLC Specifications www.merckmillipore.com For more information visit: www.merckmillipore/filterdiscs visit: For information more Product Performance Product Low extractables PTFE for clearer results extractables clearer PTFE for Low unsupported, is an hydrophilic membrane LCR all with compatible PTFE membrane The membrane solvents. HPLC used commonly to process treatment a special undergoes extractabes, residual ensuring any remove HPLC your to add anything it will not that analysis results. clearer providing solvents, LCR PTFE Membrane Filters Membrane PTFE LCR Isopore™ Membrane Filters Polycarbonate membrane for microscopy and visual analysis The Isopore™ membrane is a polycarbonate, Features & Benefits track-etched screen filter recommended for • Membrane structure retains particles on all analyses in which the sample is viewed the surface, simplifying counting and on the surface of the membrane. Isopore™ analysis membrane offers distinct advantages for the • Isopore™ membranes do not stain, resulting analysis of airborne contaminants and other in low background interference particles using optical or electron microscopy. • Non-hygroscopic, allowing for rapid drying The Isopore™ membrane is composed of and reduced sample analysis time polycarbonate film, which has a smooth, glass- • Translucent material does not require like surface for clearer sample observation. clearing for transmitted light microscopy; The unique manufacturing process of the also available in brown variety membrane ensures a precise pore diameter and a consistent pore size for accurate Applications separation of samples by size. Matched-weight Air Monitoring, Epifluorescent Microscopy, filters are not usually required because of low, Chemotaxis Assays constant tar and ash weights.

Product Performance Analytical Prepare

Specifications Color White or brown Surface Plain Wettability Hydrophilic Thickness 7-27 µm Operating Temperature 140 oC maximum Gravimetric Extractables <1.0% Sterilization Ethylene oxide, gamma irradiation, or autoclave (121 oC at 1 bar)

182 www.merckmillipore.com Analytical Prepare 183 4 ) 2 (L/min/cm Air Flow Rate Air Flow 1.3 3.5 - 7 - 8 14 19 23 49 54 55 72 3 ) 2 50 TCTP14250 100 TCTP04700 100 TCTP02500 100 TCTP01300 50 TETP14250 100 TETP04700 100 TETP02500 100 TETP01300 50 TMTP14250 30 TMTP09030 100 TMTP04700 100 TMTP02500 100 TMTP01300 50 TSTP14250 100 HTBP04700 100 TSTP04700 100 HTBP02500 100 TSTP02500 100 HTBP01300 100 TSTP01300 100 GTBP04700 100 TTTP04700 100 GTBP02500 100 TTTP02500 100 GTBP01300 (mL/min/cm Water Flow Rate Flow Water 250 250 250 2 142 47 25 13 142 47 25 13 142 90 47 25 13 142 47 47 25 25 13 13 47 47 25 Filter Diameter (mm) Diameter Filter Qty/Pk25 Catalogue No. 13 (psi) Bubble Point (µm) Color Size Pore 1 10.0 8.0 5.0 0.4 3.0 Pore Size (µm) Size Pore 2.0 Filters Membrane Brown 0.2 specifications.

Filter Code Filter VCTPGTTP WhiteGTBP 0.1 WhiteHTTP 0.2HTBP Brown 102 0.2DTTP White 51 Brown 0.4ATTP 0.4 51 White 0.5 0.6 22RTTP 6 29 White 0.8 8.7 6 White 50 1.2 8.7 50 25 8.7 40 110 TTTP White 2 4.4 90 TSTPTMTPTETP White White 3 5 White 8 0.7 - - 180 TCTP White 10 - established

not

are

and

50 RTTP14250 100 RTTP04700 100 RTTP02500 100 RTTP01300 50 ATTP14250 100 ATTP04700 100 ATTP03700 100 ATTP02500 100 ATTP01300 100 DTTP04700 100 DTTP02500 100 DTTP01300 30 HTTP09030 100 HTTP04700 100 HTTP03700 100 HTTP02500 100 HTTP01300 50 GTTP14250 30 GTTP09030 100 GTTP04700 100 GTTP03700 100 GTTP02500 100 GTTP01300 50 VCTP14250 100 VCTP04700 100 VCTP02500 100 VCTP01300 performance

typical

number.

represent C.

o catalogue

142 47 25 13 142 47 37 25 13 47 25 13 90 47 37 25 13 142 90 47 37 25 13 142 47 25 13 Filter Diameter (mm) Diameter Filter Qty/Pk Catalogue No. Values of

psi.

digits

first

measured

flow

Corresponds at 20 in water Bubble point tested at 10 psi. tested rate flow Water Air Detailed Specifications Detailed Applications Chemotaxis, bioassays, cytology, air monitoring, SEM analysis, analysis, SEM air monitoring, cytology, bioassays, Chemotaxis, sterility testing air monitoring particle monitoring, microscopy, Epifluorescent particle monitoring air monitoring, halides (AOX), organic Adsorbable air monitoring particle monitoring, microscopy, Epifluorescent analysis, gravimetric SEM analysis, light microscopy, Reflective air monitoring analysis, gravimetric SEM analysis, light microscopy, Reflective monitoring asbestos air monitoring, air monitoring cytology, bioassays, Chemotaxis, 1 2 3 4 Chemotaxis, bioassays, cytology, air monitoring cytology, bioassays, Chemotaxis, Parasitology, chemotaxis, bioassays, cytology, air monitoring cytology, bioassays, chemotaxis, Parasitology, air monitoring cytology, bioassays, Chemotaxis, 1.2 0.8 0.6 0.4 0.2 White Membrane Filters Membrane White 0.1 Ordering Information Ordering (µm) Size Pore For more information visit: www.merckmillipore/filterdiscs visit: For information more www.merckmillipore.com Nylon Membrane and Net Filters Broad chemical compatibility Nylon filters are compatible with a broad range of solvents. Two types are available: membrane filters with pore sizes ranging from 0.20 to 1.2 µm and woven net filters with mesh openings ranging from 10 to 180 µm.

Features & Benefits • Wide range of pore sizes available • Resistant to aggressive solvents

Applications Particle Removal and Clarification; Solvent Filtration; Particle Analysis; Paint Monitoring Analytical

Specifications Applications Filter Code* Pore Size Bubble Point Thickness Water Flow Rate** Open Area Prepare (µm) (psi) (µm) (mL/min/cm2) (%) Nylon Membrane Filters Sterilizing filtration, bioassays, solvent filtration GNWP 0.20 42 170 8.0 – Clarification of solutions, particle removal and analysis HNWP 0.45 30 170 14.6 – Air monitoring, particle removal and analysis ANWP 0.8 8 170 18.6 – Clarification of aqueous and organic solutions RNWP 1.2 6 170 21.2 – Nylon Net Filters Collection of algae and cells, particle analysis, large NY10 10 NA 45 – 4 particulate filtration, toxicology and drug screening on NY11 11 NA 65 – 6 C. Elegans and zebrafish, background filter for automated particle imaging systems, prefiltration of solvents, paint NY20 20 NA 55 – 14 monitoring NY30 30 NA 65 – 17 NY41 40 NA 50 – 31 NY60 60 NA 50 – 41 NY80 80 NA 75 – 41 NY1H 100 NA 80 – 44 NY2H 120 NA 80 – 49 NY4H 140 NA 120 – 43 NY6H 160 NA 100 – 53 NY8H 180 NA 135 – 47 *Corresponds to first 4 digits of catalogue number **Represent typical values

184 www.merckmillipore.com Analytical Prepare 185 NY6000010 NY1H00010 NY6H00010 XX6200006P 10010050 NY6002500 NY6004700 NY6009000 10010050 NY8002500 100 NY8004700 100 NY8009000 50 NY1H02500 NY1H04700 100 NY1H09000 10050 NY2H02500 100 NY2H04700 100 NY2H09000 50 NY4H02500 100 NY4H04700 100 NY4H09000 50 NY6H02500 NY6H04700 100 NY6H09000 10050 NY8H02500 NY8H04700 NY8H09000 25 47 90 30 cm x 3 m 1 25 47 90 25 47 90 30 cm x 3 m25 47 1 90 25 47 90 25 47 90 30 cm x 3 m25 47 1 90 Accessory blunt end, stainless Forceps, Filter steel 3 Pore Size (µm) Size Pore 60.0 (mm) Diameter Filter Qty/Pk Catalogue No. 80.0 100.0 120.0 140.0 160.0 180.0 NY1100010 NY2000010 NY4100010 10010050 NY1102500 NY1104700 100 NY1109000 10050 NY2002500 NY2004700 100 NY2009000 10050 NY3002500 100 NY3004700 100 NY3009000 50 NY4102500 NY4104700 NY4109000 10010050 NY1002500 NY1004700 NY1009000 100100100 GNWP02500 100 GNWP04700 100 HNWP02500 100 HNWP04700 100 ANWP02500 100 ANWP04700 RNWP02500 RNWP04700 25 47 90 30 cm x 3 m47 1 90 30 cm x 3 m47 1 90 47 90 30 cm x 3 m 1 25 25 25 25 47 90 25 25 25 25 47 47 47 47 41.0 11.0 20.0 30.0 Nylon Net Filters Net Nylon 10.0 0.45 0.8 1.2 0.2 Ordering Information Ordering (µm) Size Pore Filters Membrane Nylon (mm) Diameter Filter Qty/Pk Catalogue No. www.merckmillipore.com For more information visit: www.merckmillipore/filterdiscs visit: For information more Reinforced Prefilter Membrane Mixed cellulose ester with polyester support Reinforced prefilter membranes can be used Features & Benefits to remove contaminants from a variety of • Prefilter with high dirt-loading capacity for liquids and gases. The filters have a high greater throughput dirt-holding capacity and a low pressure drop, • Non-shedding substrate will not unload which makes them ideally suited for reducing fibers downstream contaminant levels in advance of sterilizing- grade filters. Reinforced prefilter membranes are made from non-shedding materials. The Applications filters retain contaminants on the surface of Prefiltration Ahead of Sterilizing-Grade Filters the cellulose membrane.

Specifications Thickness 200 µm Porosity 70% Wettability Hydrophilic Operating Temperature 70 oC maximum Sterilization Ethylene oxide or autoclave (121 oC at 1 bar) Bacterial Endotoxins <20 EU/mL Gravimetric Extractables <0.5%

Analytical Detailed Specifications Applications Filter Code* Retention Water Flow Protein Rating Rate Binding (µm) (mL/min/cm2) (µg/cm2) Prefiltration before 0.22 µm membrane filtration RW03 0.2 50 120

Prepare Prefiltration before 0.45 µm membrane filtration RW06 0.5 150 80 Prefiltration before 1.2 µm membrane filtration RW19 1.2 260 20 *Corresponds to first 4 digits of catalogue number

Ordering Information Description Filter Diameter (mm) Qty/Pk Catalogue No. Reinforced Membrane Type RW03 47 100 RW0304700 90 100 RW0309000 Reinforced Membrane Type RW06 47 100 RW0604700 90 100 RW0609000 142 50 RW0614250 Reinforced Membrane Type RW19 47 100 RW1904700 142 50 RW1914250 Accessory Filter Forceps, blunt end, stainless steel 3 XX6200006P

For more information visit: www.merckmillipore/filterdiscs

186 www.merckmillipore.com Analytical Prepare 187 Max. Temp. (°C) ) 2 65 550 75 500 120 500 52 500 140 – 140 500 59 – 55 500 85 950 50 – Weight (g/m 100 APFA04700 100 AP2501000 100 APFB02500 100 AP2501300 100 APFB03700 100 AP2502200 100 APFB04700 100 AP2502500 100 APFC02500 100 AP2504200 100 APFC04700 100 AP2504700 50 APFC09050 100 AP2509000 50 AP2512450 50 AP2514250 ) 2 – 110 60 100 Protein Binding Protein (µg/cm 4 47 10 Filter Diameter (mm) Diameter Filter Qty/Pk Catalogue No. 25 13 37 22 47 25 25 42 47 47 90 90 124 142

DOP Penetration

from ) ambient

10 a

heavy

filters APFA Filter Type Filter AP25 Binder Resin Filters without Glass Fiber APFB APFC

44.5 0.002 – 18.6 0.002 – 139 0.1 41.2 0.002 – 63.6 0.03 23.4 0.002 – 46.4 0.08 Air Flow (L/min/cm 67.5 0.002 – 44.5 0.002 – 10.6 0.10 have

the

EPA

2 US filters measuring O)

2 for the prevents

fiber

that 50 120 16 54 35 95 48 (mm of H Air Resistance 33 50 210

quartz oring method). oring (such

well-suited )

2 pure position

tal small amounts of and concentrations eacting with acidic gases. This makes quartz quartz This makes gases. acidic eacting with Our com r filters me particles air monit

Water Flow Rate Flow Water (mL/min/cm

100 AP1502500 100 AP1503700 100 AP1504200 100 AP1504700 100 AP1507500 100 AP1509000 50 AP1512450 50 AP1514250 100 AP2001300 100 AP2002500 100 AP2004200 100 AP2004700 100 AP2005500 100 AP2007500 100 AP2009000 50 AP2012450 50 AP2014250 wide

without

or in

number

475 6.0 380 1.4 470 2.7 240 1.2 700 2.2 Thickness (µm) 230 5.0 430 1.6 with

catalogue available

cm/s of

are 5.3

available

25 Filter Diameter (mm) Diameter Filter Qty/Pk Catalogue No. 37 42 47 75 90 124 142 13 25 42 47 55 75 90 124 142 or digits

4 are

fpm filters

– 0.7 2.7 1.2 0.8 - 8.0 1200 5.8 1.0 0.8 - 8.0 380 1.3 – 1.6 0.2 - 0.6 790 1.6 Retention Rating Retention (µm) first

10.5 to

disc filters

1 at

esins and can be sterilized by ethylene ethylene by be sterilized esins and can fiber fiber

Dioctyl phthalate percentage at 10.5 fpm at 10.5 Dioctyl percentage phthalate Corresponds Measured at 10 psi Meaured AP40 APFF APFD APFC AP25 APFB AP20 4 AQFA 1 2 3 Quartz Filters Fiber Glass Fiber Filters without Binder Resin Filters without Glass Fiber APFA AP15 Ordering Information Ordering Filter Code Filter Specifications Glass Fiber Filters with Binder Resin Filters with Glass Fiber Filter Type Filter AP15 Glass Fiber Filters with Binder Resin Filters with Glass Fiber AP20 ange of flow rates and throughput capacities. capacities. throughput and rates of flow ange www.merckmillipore.com oxide, gamma irradiation or autoclaving. or autoclaving. irradiation gamma oxide, binder r For contamination analysis Glass r Glass Glass & Quartz & Glass Filters Fiber Ordering Information - Continued Filter Type Filter Diameter (mm) Qty/Pk Catalogue No. Filter Type Filter Diameter (mm) Qty/Pk Catalogue No. APFD 25 100 APFD02500 Quartz Fiber Filters 47 100 APFD04700 AQFA 37 100 AQFA03700 90 50 APFD09050 47 100 AQFA04700 APFF 25 100 APFF02500 90 50 AQFA09050 47 100 APFF04700 8 in. x 10 in. 50 AQFA8X105 90 50 APFF09050 Accessory 142 50 APFF14250 Filter Forceps, blunt end, stainless steel 3 XX6200006P AP40 10 100 AP4001000 24 500 AP4002405 25 100 AP4002500 37 500 AP4003705 70 100 AP4007000 90 100 AP4009000 142 50 AP4014250

For more information visit: www.merckmillipore/filterdiscs

Polypropylene Prefilters and Membranes

Analytical Broad thermal compatibility Polypropylene filters are constructed of 100% Features & Benefits virgin polypropylene media. They are designed • Compatible with aggressive solvents for general clarification and prefiltration • Prefilters have high dirt-holding capacity

Prepare of solvents, deionized water and bioburden reduction. They also provide broad thermal Applications compatibility. The prefilters provide high General Prefiltration and Clarification, particle retention, high dirt-holding capacity Suitable for Organic Solvents and low pressure drop. They are designed for filtration of organic solvents, but they can also be used, once properly wetted with alcohol, for the filtration of aqueous solutions.

188 www.merckmillipore.com Analytical Prepare 189 90 90 90 90 90 100 N/A 90 100 Max. Temp. (°C) 200 110 150 140 140 430 450 140 360 Thickness (µm) 30 5 10 2.5 1.2 45 80 0.6 25 (µm) AN3H AN50 AN1H AN25 AN12 PP45 PP80 AN06 PP25 Filter Code*Filter Size Pore 100100 PP2502500 100 PP2504700 100 PP4502500 100 PP4504700 PP4509030 100100 AN0604700 100 AN1204700 100 AN2504700 100 AN5004700 100 AN1H04700 AN3H04700 3 XX6200006P 25 47 25 47 90 47 47 47 47 47 47 25 45 0.6 1.2 2.5 5 10 30 Pore Size (µm) Size Pore (mm) Diameter Filter Qty/Pk Catalogue No. number

catalogue

digits

Prefilter first

olypropylene Net Filter Net olypropylene P Clarification of aqueous and nonaqueous solutions and nonaqueous of aqueous Clarification Collection of cells and precipitates Clarification of aqueous solutions, prefiltration upstream of membrane filters of membrane upstream solutions, prefiltration of aqueous Clarification 0.8 – 8.0 µm of sizes pore with Filter Forceps, blunt end, stainless Forceps, Filter steel Accessory Clarification of aqueous solutions, prefiltration upstream of membrane filters of membrane upstream solutions, prefiltration of aqueous Clarification 0.5 – 2.0 µm of sizes pore with contamination analysis particleLarge removal, Ordering Information Ordering Description Polypropylene *Corresponds Collection of cells and protein precipitates Collection of cells and protein Polypropylene Net Filters Net Polypropylene Clarification of aqueous solutions, prefiltration upstream of membrane filters of membrane upstream solutions, prefiltration of aqueous Clarification 0.2 – 0.6 µm of sizes pore with Specifications Applications Polypropylene Prefilters Polypropylene www.merckmillipore.com For more information visit: www.merckmillipore/filterdiscs visit: For information more Swinnex® Filter Holders Reusable, syringe-driven filter unit Make your own syringe filter! Swinnex® Features & Benefits filter holders give you the flexibility to select • Available in three sizes for a range of the membrane type and diameter that are process volumes most appropriate for your application. For • Autoclavable with filter in place for sterile sterile filtration, autoclave your Swinnex® filtration filter holders with the membrane in place. Ultraclean or sterilize small volumes of liquids Applications dispensed by a syringe or install in-line to Ultracleaning or Sterilization of Liquids filter larger volumes.

Specifications Swinnex® 13 Filter Holder Swinnex® 25 Filter Holder Swinnex® 47 Filter Holder Materials Housing Polypropylene Polypropylene Polypropylene Seal Material Silicone Silicone Silicone Maximum Differential Pressure, bar (psi) 3.5 (50) 3.5 (50) 5.2 (75) Dimensions Length, cm 3.5 3.7 5.4 Diameter, cm 1.7 3.2 5.8 Filter Diameter, mm 13 25 47 Prefilter Diameter, mm 10 (thick depth prefilter) 22 (thick depth prefilter) 42 (thick depth prefilter) or 47 (membrane prefilter) Analytical Filtration Area, cm² 0.7 3.4 13.8 Fitting Inlet Female Luer-Lok® Female Luer-Lok® 1/4 in. NPTM Fitting Outlet Male Luer-Slip™ Male Luer-Slip™ Female Luer-Slip™ Sterilization Method Autoclavable with filter in place

Prepare *Use locking inlet connection to avoid leakage at high pressures with < 10 mL syringes **Accessory stopper, XX2004718, fits outlet connector for seating in 1 L flask, XX1004705.

Ordering Information Description Qty/Pk Catalogue No. Swinnex® Filter Holder, 13 mm 10 SX0001300 Swinnex® Filter Holder, 25 mm 12 SX0002500 Swinnex® Filter Holder, 47 mm 8 SX0004700 Replacement Parts Silicone Gaskets for 13 mm Swinnex® Filter Holder 100 SX0001301 Silicone Gaskets for 25 mm Swinnex® Filter Holder 100 SX0002501 Support Screen, 25 mm, polypropylene 30 XX2702510 Support Screen, 47 mm, polypropylene 1 XX1104715 O-ring (5-329) for Swinnex® 47 mm Filter Holder, silicone 10 XX1104707 Base O-ring for Swinnex® 47 mm Filter Holder, silicone 5 SX0004701 Replacement Parts Kit for Swinnex® 47 mm Filter Holder 1 SX00047RK* *Replacement Parts Kit for Swinnex® 47 mm Filter Holder includes: (10) base O-rings, (10) silicone filter O-rings, (10) fluoroelastomer O-rings, (10) EP filter O-rings, (10) polypropylene filter support screens, (2) tightening wrenches

For more information visit: www.merckmillipore.com/LabHardware

190 www.merckmillipore.com Analytical Prepare 191

process ensures sterile

for

XX3001200 XX3002500 XX3002514 XX30012RK XX30025RK Liquids

range of

place

in Luer-Lok®

construction

for

filters filter

steel Microsyringe Stainless steel Stainless PTFE 25 3.9 22 6.9 (100) 3.2 3.2 Female Male Luer-Slip™

sizes Qty/Pk1 Catalogue No. 1 1 1 1

Sterilization

with

two or

syringe

place in

stainless

ation filter

ears of use, decreasing waste from from waste ears of use, decreasing olumes v y disposable filtr with

Applications Ultracleaning Features& Benefits • Available • Autoclavable • Durable steel

Luer-Lok®

stainless

Swinny Stainless steel Stainless PTFE 13 0.8 10 6.9 (100) 3.3 1.6 Female Male Luer-Slip™ Autoclavable in

the

volumes

autoclave

Luer-Lok®,

you Microsyringe

small

membrane

give

25 mm,

filter! the filtration,

sterilize

with Holder

holders or

sterile

syringe

volumes.

Filter For

filter

own holders

larger

Ultraclean your

filter

Swinny

filter xibility type membrane select the to and uids dispensed by a syringe or install in-line a syringe by uids dispensed

Seals mm Diameter, Filter cm² Area, Filtration mm Diameter, Prefilter bar (psi) Pressure, Maximum Inlet Dimensions cm Length, cm Diameter, Fittings Inlet Fitting Outlet Fitting Sterilization Materials Housing Specifications Swinny 13 mm Replacement Parts Kit* 13 mm Replacement Swinny support and (4) stainless screens. steel gaskets, (4) PTFE support*Parts (4) PTFE O-rings, screen Kit includes and (2) wrenches. support (2) stainless screens, steel gaskets, (2) PTFE support**Parts (2) PTFE O-rings, screen Kit includes Replacement PartsReplacement Ordering Information Ordering Description Holder 13 mm, stainless Filter steel Swinny Microsyringe stainless steel Holder 25 mm, NPTF, Filter Microsyringe Parts Kit** 25 mm Replacement Microsyringe our www.merckmillipore.com For more information visit: www.merckmillipore.com/LabHardware visit: For information more to y place. liq fle your for appropriate most are that diameter application. Reusable, syringe-driven filter unit filter syringe-driven Reusable, Make and Stainless Steel Syringe Filter Holders Filter Syringe Steel Stainless In-Line Filter Holder Reusable pressure-driven filter housing Ultraclean liquids or gases in line by pressure Applications filtration. Simply open the housing to change General Filtration (in-line) the filter without disturbing the attached plumbing.

Features & Benefits • Stepped hose barb connections at both ends for easy installation • Large, easy to grip hand knobs

Specifications Materials Filled polypropylene; nylon hand knobs Filter Diameter, mm 47 Filtration Area, cm² 13.8 Prefilter Diameter, mm 42 (thick depth prefilter) or 47 (membrane prefilter) Maximum Inlet Pressure, bar (psi) 5.5 (80) Maximum Operating Temperature, °C 70 at 5.5 bar Fittings, Inlet/Outlet 1/4 in. NPTF; connector for 6 mm to 9.5 mm I.D. flexible tubing Dimensions Length, cm 12 Diameter, cm 7.6 Analytical

Ordering Information Description Qty/Pk Catalogue No.

Prepare In-Line Filter Holder, 47 mm 1 XX4304700 Replacement Parts Hose Adapter, 1/4 in. NPTF to 5/16 in. I.D., polypropylene 2 XX4304704 O-ring (2-131), silicone 6 XX4304701 Hand Knobs 3 XX4304705 Accessories Stainless Steel Support Screen, 47 mm 1 XX4304707 PVC Tubing, 3 m, 9.5 mm I.D. 1 XX6700034 O-ring (2-131), fluoroelastomer 6 XX4304702 Filter Forceps, blunt end, stainless steel 3 XX6200006P

For more information visit: www.merckmillipore.com/LabHardware

192 www.merckmillipore.com Analytical Prepare 193 prefilter)

(membrane

) female female**

prefilter

(UNF-3B) (UNF-3B)

depth

(thick

7/16 in.-20 7/16 in.-20 Stainless steel Stainless Fluoroelastomer 47 9.6 10.3 (150) 103 (1500) 689 (10,000) 4.4 8.6 47 mm HP Holder

with

) 42 to

PTFE-coated

prefilter

autoclaving

depth

pressures optional

NPTF NPTF*

(thick inlet

Stainless steel Stainless Silicone 47 13.8 1/4 in. 1/4 in. – – 19 (275) 2.7 7.6 47 mm Holder requires

with

autoclavable;

) 22 use are place

t screens in for

prefilter

er 700 bar filt suppor depth • Devices Applications Streams In-Line Filtration of Fluid Process Features& Benefits • Safe

NPTF NPTF

(thick

25 mm HP Holder 1/8 in. Buna-N 25 2.2 10 Stainless steel Stainless 10.3 (150) 69 (1000) 345 (5000) 3.2 5.1 1/8 in. is All at

filter used.

place, liquids

The screen

in y be equipped y be equipped or

type

plug

XX4404700)

filter

pipe gases filter

no.

support

included of

with with High-Pressure the autoclaved.

(cat. are

filter

be on port

NPTF

47 filter. may filtration holder

1/4”

autoclaved upstream to for The

depends upstream

he optional PTFE-coated support PTFE-coated he optional

filter

NPTF holders the

used. t pressures of 20 to 700 bar. Differential 700 bar. of 20 to t pressures dapters

er uipped with a back-pressure support screen. support screen. a back-pressure uipped with may

Outlet *1/8” Gaskets mm Diameter, Filter cm² Area, Filtration mm Diameter, Prefilter Specifications Materials Housing Maximum Pressure Differential, bar gauge (psig) Differential,Maximum Pressure bar gauge 3.0, 5.0 and 8.0 µm MCE filters With 3.0, filters all membrane except With 5.0 and 8.0 µm MCE filters bar (psi) Pressure, Maximum Inlet Dimensions Height, cm cm Diameter, Fittings Inlet **A www.merckmillipore.com with holder (cat. no. XX4504700) ma holder (cat. no. XX4504700) long as t pressure In-Line eq It XX4404704) (cat. no. XX4404702 and screens are back-pressure (cat. no. XX4504704) for protection inle filt For in-line filtration, up to 700 bar to Forup filtration, in-line Holders Filter are Steel Stainless The In-Line designed In-Line Stainless Steel Filter Holders 47 mm) and (25 Filter Steel Stainless In-Line Ordering Information Description Qty/Pk Catalogue No. HP Filter Holder, 25 mm, stainless steel 1 XX4502500 Filter Holder, 47 mm, stainless steel 1 XX4404700 HP Filter Holder, 47 mm, stainless steel 1 XX4504700 Replacement Parts For 25 mm HP Filter Holder Replacement Parts Kit, 25 mm filter holder 1 XX45025RK* For 47 mm Filter Holder Back-pressure Screen, 47 mm, stainless steel 1 XX4404703 O-ring (2-131), silicone 6 XX4304701 Support Screen, 47 mm 1 XX4204709 Underdrain Screen, 47 mm 1 5614 Pipe Plug, 1/8 in. 1 009507P For 47 mm HP Filter Holder Replacement Parts Kit, 47 mm filter holder 1 XX45047RK** Filter Support Screen, 47 mm, stainless steel 1 XX4504704 Inner O-ring, 47 mm, PTFE-treated fluoroelastomer 5 XX4504705 Inner O-ring, 47 mm, PTFE 5 XX4504710 Outer O-ring, 47 mm, fluoroelastomer 10 XX4504713 Note: complete replacement parts information available online. Accessories for 47 mm Filter Holder

Analytical PTFE-coated HP Support Screen, 47 mm, stainless steel 1 XX4404702 Vent/Relief Valve 1 XX4204708 Hose Adapter, 1/4 in. NPTM to 3/8 in. I.D. 1 XX2504705 * Includes (3) stainless steel hex-cap screws, (3) LCR-treated Buna-N O-rings (2-018), (1) stainless steel support screen, (1) Allen™ wrench Prepare ** Includes (6) each of inlet/outlet adapter, adapter O-ring (2-111), hex-cap screw, inner O-ring, outer O-ring

For more information visit: www.merckmillipore.com/LabHardware

194 www.merckmillipore.com Analytical Prepare 195

pressure

Flange

YY3009053 YY3009054 YY2209058 YY2209059 YY2214257 YY2004076 YY2004055 YY2004045 YY3029366 YY2214253 YY2214265 YY3029307 YY3014234 YY2214258 YY2214251 YY2214252 YY3009000 YY3014236 XX4204708 XX2504705 vent/relief

gases

Sanitary

place.

or NPTM

in.

h clamps and adapters and adapters h clamps ve attachment ve 6.4 (14.1) val 1/8 1-1/2 in. wit hose 14 mm I.D. supplied for 18.4 7 (100) 14 (200) 142 124 97 142 PTFE 316 stainless steel with with 316 stainless steel aluminum legs anodized 142 mm Holder liquids

4 1 1 3 1 1 10 1 1 1 4 1 1 1 3 1 1 1 1 1 Qty/Pk Catalogue No.

membranes

sterilize

with

vent/relief

with

NPTM NPTF

ultraclean in.

ve attachment ve autoclaving to

2.8 (6.2) 1/8 val connections supplied for connections supplied for 9.5 mm I.D. hose 1/4 in. 12.1 17.1 (plus inlet connector)17.1 (plus inlet connector) 27 (plus inlet 5 (75) 19 (275) 90 75 45.5 90 PTFE 316 stainless steel with with 316 stainless steel aluminum legs anodized 90 mm Holder permit PTFE-coated

designed

I.D.

are

holder,

screens

PTFE 3/8 in.

filter

holders

wrench (125 psig)

14 mm support

housing, NPTM

142 mm 90 mm to

gasket

filter

142 mm

9 bar TC

filter 3/16 in. for TC

1/4 in. steel

in. silicone

Support, Support,

Valve, Valve

Caps, PTFE-faced

TC 1/2 Screen 90 mm

1-1/2 in.

in. Adapter,

with

stainless ation.

ent/Relief Shipping Weight, kg (lb) kg Shipping Weight, Vent/Relief Valve Vent/Relief Connections Inlet/Outlet Fittings, Diameter, cm (in) Diameter, Dimensions Height, cm (in) Maximum Differential bar (psid) Pressure, Maximum Inlet Pressure, bar (psi) Pressure, Maximum Inlet Type RW Type Prefilter Diameter, mm Diameter, Prefilter AP Type Filtration Area, cm² Area, Filtration Filter Diameter, mm Diameter, Filter O-rings Materials Holder Specifications Support Screen, PTFE-coated Support Screen, Underdrain Support Legs Adapter 1-1/2 Clamp Vent/Relief (2-251), PTFE O-ring (2-251), silicone O-ring 316 holder Deflector, Flow Inlet Support Underdrain Legs 142 mm holder for Handwheel Wrench online. is available part information replacement complete Note: 142 mm Holder Replacement Parts142 mm Holder Replacement Hand Knob 90 mm Holder Replacement Parts90 mm Holder Replacement Hose Ordering Informaiton Ordering Description 90 mm, stainless steel Holder, Filter Filter Holder, 142 mm, stainless steel Holder, Filter O-rings, V For more information visit: www.merckmillipore.com/LabHardware visit: For information more www.merckmillipore.com Filter holders supportedFilter legs by The filtr Standing Stainless Steel Filter Holdersmm) 142 and (90 Filter Steel Stainless Standing Stainless Steel Pressure Filter Holders Pressure-driven filtration of up to 340 mL of liquid These filter holders are ideal for ultracleaning Features & Benefits or sterilizing batch volumes of liquids. The • Filter holder has reservoir to hold 100 mL cylindrical barrel holds 100 mL (or 340 mL) (or 340 mL) of liquid for batch filtration of liquid, which is filtered by externally • Autoclavable with membrane in place for applied gas pressure through a suitable filter sterile filtration supported on the holder base. Applications Batch Filtration

Specifications Materials Stainless steel barrel, base, filter support screen, top cap, and tubing adapter; anodized aluminum locking ring; silicone stopper Filter Diameter, mm 47 Filtration Area, cm² 11.3 Prefilter Diameter, mm 42 (thick depth prefilter) or 47 (membrane prefilter) Maximum Inlet Pressure, bar (psi) 6.9 (100) Connections Inlet 9.5 mm (3/8 in.) hose connector Outlet No. 8 perforated silicone stopper mounts in standard 1 L filtering flask Dimensions Height, cm (100 mL holder) 29.2 Analytical Height, cm (340 mL holder) 53.3 Diameter, cm 7

Prepare Ordering Information Description Qty/Pk Catalogue No. Stainless Steel Pressure Filter Holder, 47 mm, 100 mL 1 XX4004700 Stainless Steel Pressure Filter Holder, 47 mm, 340 mL 1 XX4004740 Replacement Parts Top Cap with Hose Adapter 1 000205 Locking Ring Gasket, PTFE 5 XX4004714 O-rings, PTFE 5 XX4004716 Filter Support Screen, stainless steel, 47 mm 1 XX4004704 Gasket, PTFE 25 XX2004703 No. 8 Stopper, 9.5 mm (3/8 in.) hole, silicone 5 XX2004718 Replacement Parts Kit, 47 mm holder 1 XX40047RK* *Replacement Parts Kit includes: (5) top cap O-rings, (5) locking ring gaskets, (5) filter sealing O-rings, (5) screen gaskets Note: complete replacement part information is available online. Accessories Vacuum Filtering Flask, 1 L 1 XX1004705 Vaccum Filtering Flask, 4 L 1 XX1004744 Tubing, 3/16 in. I.D. x 140 cm, silicone 1 XX7100004 3 m PVC tubing with 9.5 mm I.D. 1 XX6700034

For more information visit: www.merckmillipore.com/LabHardware

196 www.merckmillipore.com Analytical Prepare 197

all

tubing

buffer

and pour buffer

access and

FHUP04700 to

plug

allows need

filtered

solvent

the

1 XX7100004 1 XX1004704 1 XT1200000 100 HVLP04700 100 1 XX1004703 1 XX1504705 1 XX1604701 1 XX1504732 1 XX1504702 1 XX1604706 11 XX1604700 XX1604705 Qty/Pk Catalogue No. includes buffer

bottom

flask

eliminating flask continuous

L eliminates

2 solvent

filtration

plain

ation receiving storage, ing

ered L ansfer, reducing labor and lessening reducing ansfer,

or white, contamination risk filtr dur f tr filt

Features& Benefits • Automatic, • 2 • Conical Applications BufferMobile Phase Preparation, Filtration 47 mm,

add

to which 0.45 µm,

300

fluids.

liquid

the filtration

need

progresses. of

no 70 cm particles

silicone is

hydrophilic,

hazardous Vacuum

PTFE, required,

filtration filtration is

there

PVDF,

vacuum. life.

140 cm,

eliminate

x the

filtering to

under

I.D. for

filtration funnel column

filtration,

filter

Membrane, a Solvent/Buffer, when

filtration

3/16 in. eplaces the vacuum cover. eplaces the for

into funnel

designed

shorten

h the MilliSolve™ system also removes a also removes system MilliSolve™ h the uid Glass Funnel, 300 mL, borosilcate 300 mL, Glass Funnel, Tubing, Durapore® Tubing Fluoropore™ Membrane, PTFE, hydrophobic, unlaminated, 0.5 µm, unlaminated, PTFE, hydrophobic, Membrane, Fluoropore™ plain 47 mm, white, Spring Clamp, 47 mm, aluminum Clamp, Spring Ground Joint Flask, 1 L Ground Vacuum Cover, ground glass ground Cover, Vacuum Glass Base and Cap with Stainless Steel Screen, 47 mm Screen, Steel Stainless Glass Base and Cap with Glass Base and Cap, 47 mm* *Higher porosity stainless steel support screen (XX1504732 instead of glass support, XX1504702) is easier to clean and support, XX1504702) is easier to of glass (XX1504732 instead support*Higher porosity stainless screen steel improves Vacuum Flask, 5 L, with conical bottom, glass conical bottom, Flask, 5 L, with Vacuum Accessories MilliSolve™ Filtration System, complete with 2 L flask 2 L with Filtration complete System, MilliSolve™ PartsReplacement Flask, 2 L Vacuum Ordering Information Ordering Description iltration occurs in a closed system, which is which occursiltration in a closed system, hromatography buffers and solvents hromatography www.merckmillipore.com For more information visit: www.merckmillipore.com/LabHardware visit: For information more open funnel r liq F important can wit buffers, from portion gases of dissolved large of air bubbles interfering risk the reducing and automatic With instruments. LC with continuous c µm uses a 0.45 The system under vacuum. membrane Bottle-to-bottle filtration of solvents and buffersof solvents filtration Bottle-to-bottle has Filtration System The MilliSolve™ been MilliSolve™ Filtration System Filtration MilliSolve™ Millicup™ Filter Unit Vacuum-driven, disposable bottle-top filtration The Millicup™ Filter Unit provides fast and Applications effective filtration of solvents and buffers Mobile Phase Preparation, Buffer Filtration (up to 3 L) used in HPLC and other analytical techniques.

Features & Benefits • No preparation or clean-up saves time • Available with low-protein binding hydrophilic Durapore® PVDF, hydrophobic Fluoropore™ PTFE, and hydrophilic LCR PTFE membranes • Ultraclean design and safe operation

Specifications Funnel Material HDPE Outlet Fitting Universal bottle top Filtration Area, cm² 11.5 Process Volume, mL 3000 Vacuum Limit 685 mm Hg differential at 25 °C Maximum Operating Temperature, °C 50 Dimensions

Analytical Height, mm 120 Diameter, mm 84

Prepare Ordering Information Description Filter Pore Size Filter Material Qty/Pk Catalogue No. (µm) Millicup™ Filter Unit, 300 mL 0.45 Hydrophobic PTFE 10 SJFHM4710 Hydrophilic PVDF 10 SJHVM4710 Hydrophilic PTFE 10 SJLHM4710

For more information visit: www.merckmillipore.com/LabHardware

198 www.merckmillipore.com Analytical Prepare 199

through

formation

results

the

certified

and

without analytical

50 pc 1.02016.0001 30 pc30 pc50 pc 1.19870.0001 50 pc 1.19941.0001 50 pc 1.02014.0001 50 pc 1.19847.0001 30 pc 1.02021.0001 1.19698.0001 50 pc 1.19127.0001 1.19767.0001 Qty/Pk Catalogue No. of

validated

posable cartridges recoveries

acturing precision

High emulsion use of dis manuf PP Applications Solid Phase Extraction Features& Benefits • Higher • Optimized, •

mg,

PP PP PP

PP 200

PP 3 mL, mL,

mL mL

6 3 3 µm), mL 3

3 mL

3 mg,

6 selective

mg, mg, mg, mg,

mg,

mg, 200

mg, the (40-63

200 200 200 300

200 is

200

µm),

1.000 µm), µm), µm),

µm),

µm), (40-63 endcapped

columns

(40-63 (40-63

(40-63 (40-120 (40-120

(40-63

TSC CN SCX EN EN RP-18 RP-18 Si

(150-250

olut® LiChrolut®

LiChrolut® Ordering Information Ordering Description LiChrolut® LiChr LiChrolut® LiChrolut® LiChrolut® LiChrolut® Florisil® LiChrolut® xtraction of the components of interest from from of interest components of the xtraction www.merckmillipore.com For more information visit: www.merckmillipore.com/lichrolut visit: For information more e sample larger or much sample a complex analysis actual (e.g., HPLC, to prior volume As solid-phase extraction on works GC, TLC). this chromatography, of liquid principle the but reversible strong using by is achieved and surface analyte the between interactions interactions stationaryof the phase. Typical forces), der Waals (i.e., Van hydrophobic are bonding, dipole-dipole polar (i.e., hydrogen interactions. ion exchange and forces) stationary between phase and Interaction occur. should not matrix Reliable and rapid solid-phase extraction solid-phase rapid and Reliable The primary extraction goal of solid-phase with LiChrolut® Columns LiChrolut® EXtrelut® Columns Liquid-liquid extraction in its most effective form EXtrelut® NT sorbent columns simplify liquid- Features & Benefits liquid extraction by replacing separation • Save solvents funnels. Using a single step is more efficient • Easy to use and saves solvent, material and time in • Highly efficient contrast to classical funnel separation. With its intuitive working principle, higher recovery Applications and cleaner extraction can be achieved. The Liquid-Liquid Extraction aqueous sample is simply applied to the EXtrelut® NT sorbent, which distributes itself as a thin film over the chemically inert matrix and thus acts as a stationary phase.

Ordering Information Description Qty/Pk Catalogue No. EXtrelut® NT1 100 columns 1.15094.0001 EXtrelut® NT3 50 columns 1.15095.0001 EXtrelut® NT20 25 columns 1.15096.0001 EXtrelut® NT refill packs 50 bags 1.15093.0001

For more information visit: www.merckmillipore.com/extrelut Analytical Filter Forceps For safe handling of filter membranes Prepare Ensure your filtration membranes are not Applications damaged during handling by using these Gentle Handling of Membranes blunt-ended forceps.

Features & Benefits • Beveled, unserrated tips to protect delicate membrane surfaces • May be sterilized by autoclaving or flame-sterilization

Ordering Information

Description Qty/Pk. Catalogue No. Filter Forceps, blunt end, stainless steel 3 XX6200006P

For more information visit: www.merckmillipore.com/LabHardware

200 www.merckmillipore.com Analytical Prepare 201

and

Units

perforated XX7100004 WP6122050 XX1002505 XX1002508 WP6111560 WP6110060 XX1004705 XX1004744 XX1004708

Holders

stopper

5 No.

Sterifil®

Filter

No.

with

accept Qty/Pk Catalogue No. 1251 XX1102012 1 XX1105005 XKEM00107 1 XX6200035 4 XKEM00104 1 XX1102503 XX6200036

Qty/Pk Catalogue No. 1 1 1 5 1 1 1 1 5 Using

accepts

flasks

L Filtration

flask 4

Filtration

and

topper In-Field

s her Filter Holdersher Filter Ot hand vacuum pump. For sterile applications, For sterile hand vacuum pump. vacuum syringe stainless steel an autoclavable is available. valve two-way stainless steel, with Applications Filtration, Hand Pump Liquid Small-Volume for Applications Vacuum • 1 • 125

small with

liquids units

various

filter source

with

or dispense

Hz Hz

silicone to

pressure-filter

V/60 V/50 V/50-60 vacuum

nylon

holders

to and

115 220 100

procedures

140 cm,

syringes x

filter

I.D. Pump, Pump, Pump,

Luer-Lok®,

hose

connects

filtration

containers Duty Duty Duty

I.D.

male

through

3/16 in.

arm

in.

holders.

small polypropylene

h female Luer-Slip™ inlets. Syringes have have Syringes inlets. Luer-Slip™ h female er o o. 5 Perforated Stopper, silicone Stopper, o. 5 Perforated 3/8 vacuum

eatures & Benefits eatures *Replacement Parts Kit includes: (1) plunger O-ring, (2) valve O-rings, (2) stainless steel ball valve balls, (2) barrel balls, (2) barrel ball valve (2) stainless steel O-rings, (2) valve Parts O-ring, Kit includes: (1) plunger *Replacement springs. valve seals, and (2) compression (2) PTFE valve springs, valve compression Replacement Parts: Stainless Steel Pump Steel Parts:Replacement Stainless Parts Kit* Replacement Plastic Syringes, 50 mL Luer-Slip™, polypropylene 50 mL Luer-Slip™, Syringes, Plastic polypropylene Pump, Hand Vacuum stainless steel Pump, Hand Vacuum Pump Parts:Replacement Polypropylene plastic Valve, Vacuum 2-way Adapter, Plastic Syringes, 20 mL Luer-Slip™, polypropylene 20 mL Luer-Slip™, Syringes, Plastic Ordering Information Ordering Description N Chemical Tubing, Accessories Ordering Information Ordering Description Flask, 125 mL Filtering Vacuum Vacuum Filtering Flask, 1 L Filtering Vacuum silicone Stopper, 8 Perforated No. Chemical Vaccum Filtering Flask, 4 L Filtering Vaccum Chemical olumes For more information visit: www.merckmillipore.com/LabHardware visit: For information more www.merckmillipore.com male Luer-Slip™ outlets. An optional two- An optional outlets. male Luer-Slip™ Luer male-to-male nylon vacuum valve, way male Luer tubing with and rubber adapter a into syringe standard this converts inlet For more information visit: www.merckmillipore.com/LabHardware visit: For information more v wit For hand-held filtration of small volumes For of small hand-held filtration Use int Hand Vacuum Pump and Syringes and Pump Hand Vacuum • Side filt F These side-arm flasks are designed for use designed flasks are These side-arm in For vacuum filtration with filter holders filter with filtration For vacuum Filtering Flasks Filtering High Output / Chemical Duty Pumps For flow rates up to 37 L/min The High Output and Chemical Duty pumps Features & Benefits can support high flow rates to decrease • High flow rates decrease filtration process process filtration time. The High Output time Pump features a piston-driven design for • Optional chemical-resistant internal greater power. The Chemical Duty Pump has components for compatibility with a chemically-resistant head and diaphragm corrosive vapors for use with corrosive chemicals and solvents. • Portable design allows for easy sharing Both pumps come with 70 cm of 1/4 in. between workstations

tubing and a Millex®-FA50 filter for in-line moisture protection. Both are UL-listed and Applications CE-marked. Vacuum Source for Filter Holders, Manifolds, and Devices, including Samplicity® Filtration System, SNAP i.d.® 2.0 System,

Multiscreen®HTS Filtration Manifold, and Stericup® Filters

Specifications High Output Pump Chemical Duty Pump Maximum Vacuum, mbar (in. Hg) 921 (27.2) 813 (24) Maximum Pressure, bar (psig) 5.4 (80) 2.45 (35) Maximum Flow Rate, L/min (CFM) 34 (1.2) 37 (1.3) Materials (pump head, housing, regulator) Cast aluminum Cast aluminum Analytical Weight, kg (lbs) 5.3 (11.7) 4.1 (9.0) Dimensions, cm (in.) 20.3 x 22.9 x 25.4 17.8 x 17.8 x 20.3 H x W x L (8 x 9 x 10) (7 x 7 x 8) Connections 1/4 in. Stepped hose barb 1/4 in. Stepped hose barb Prepare

Ordering Information Description Qty/Pk Catalogue No. Chemical Duty Pump, 115 V/60 Hz 1 WP6111560 Chemical Duty Pump, 220 V/50 Hz 1 WP6122050 Chemical Duty Pump, 100 V/50-60 Hz 1 WP6110060 High Output Pump, 115 V/60 Hz 1 WP6211560 High Output Pump, 220 V/50 Hz 1 WP6222050 High Output Pump, 100 V/50-60 Hz 1 WP6210060 Replacement Parts Chemical Duty Pump Maintenance Kit* 1 WP61MNT00 Chemical Duty Pump Rebuild Kit** 1 WP61RBD00 High Output Pump Maintenance Kit* 1 WP62MNT00 High Output Pump Rebuild Kit** 1 WP62RBDNA *Maintenance Kit includes: (1) diaphragm, (1) diaphragm liner, (1) O-ring gasket, (4) head screws, (2) handle screws **Rebuild Kit includes: (1) diaphragm hold-down plate assembly, (1) valve plate assembly, (1) pump head, (1) vacuum gauge, (1) pressure gauge, (4) head screws, (2) handle screws

For more information visit: www.merckmillipore.com/LabHardware

202 www.merckmillipore.com Analytical Prepare

203 a

has

gas-tight,

pump

Maxi ed diaphragm, and is compatible and is compatible ed diaphragm,

PTFE-coat gases or corrosive aggressive slightly with and vapors • Millivac™ Applications and Aqueous Water for Millivac™ Mini Pump Larger for Pump Solutions; Millivac™ Maxi or Viscous Solutions Volumes

intrusion.

water

applications.

against

conserves

11 XX5411560 1 SD1P014M04 XF5423050 11 XF5423055 XF5423056 11 XX7100004 5 XX1004705 XX2004718 Qty/Pk Catalogue No. 10 SLFG05010 protect

laborabory

to required design

filter not

other

50 Millivac™ Maxi Millivac™ PTFE-coated diaphragm with FFPM with diaphragm PTFE-coated valves 16 36.1 9 14.1 3.95 h space and compact

ented diaphragm design has reduced design has reduced diaphragm ented Millex®-FG

a lab benc

• Quiet, Millivac™ Mini and Maxi vacuum pumps and Maxi vacuum pumps Millivac™ Mini that pumps maintenance-free compact, are of vacuum for source a consistent provide filtration A pat to compared pumps of the footprint the models. conventional Features & Benefits • Lubrication and

50 mm

tubing

PTFE,

vacuum

silicone

hydrophobic

hole,

silicone with Millivac™ Mini Millivac™ Ryton head; EPDM membrane with with head; EPDM membrane Ryton valves fluoroelastomer 6 tubing 4 mm I.D. for Hose connector 6 mm I.D. tubing for Hose connector 22.6 9 14.1 1.9

come (3/8 in.)

0.20 µm,

140 cm,

Unit, pumps

9.5 mm

Filter

Millivac™ 3/16 in.

Stopper,

All

eplacement Parts ubing, R Millivac™ Maxi Vacuum Pump, 230 V Pump, Millivac™ Maxi Vacuum 230 V Pump, Millivac™ Mini Vacuum Kit* Millivac™ Mini PTFE Maintenance Flask, 1 L Filtering Vacuum No. 8 Millex®-FG Millivac™ Mini EPDM Maintenance Kit* Millivac™ Mini EPDM Maintenance depends on kit type. Materials 2 gaskets. 2 valves, Kit includes 1 diaphragm, *Maintenance Accessories Note: Description 115 V Pump, Millivac™ Mini Vacuum Ordering Information Ordering Materials L/min Max. Vacuum, Fittings Dimensions cm Length, cm Width, Height, cm kg Weight, Specifications T www.merckmillipore.com Maintenance-free, mini-diaphragm pumps mini-diaphragm Maintenance-free, Millivac™ Vacuum Pumps Vacuum Millivac™ For more information visit: www.merckmillipore.com/LabHardware visit: For information more Dispensing Pressure Vessels For dispensing liquid volumes up to 20 L Dispensing pressure vessels hold liquid for Applications filtration through pressure-driven filter holders. Large-Volume Filtration, Reservoir for Buffer or Solvent Dispensing Features & Benefits • Vessels meet ASME®-UM Code requirements • Closures are secured by cam-lock handle • Accessories include pressure gauges, hose connectors, and code-complying pressure relief valves

Specifications Materials 316 stainless steel body; stainless steel fittings; fluoroelastomer gaskets and O-rings; molded Neoprene rubber base Fittings 1/4 in. NPTF elbow fittings for inlet and outlet (labeled); 1/4 in. NPTF for pressure gauge and vent/relief valve attachment Maximum Inlet Pressure, bar (psi)* 6.9 (100) Maximum Temperature, °C 121 *Locally applicable pressure vessel codes should be consulted for maximum allowable pressure. Do not use vessels under vacuum without accessory vacuum closure.

Ordering Information Analytical Description Qty/Pk Catalogue No. Dispensing Pressure Vessel, 1 gal 1 XX6700P01 Dispensing Pressure Vessel, 5 L 1 XX6700P05 Dispensing Pressure Vessel, 10 L 1 XX6700P10

Prepare Dispensing Pressure Vessel, 20 L 1 XX6700P20 Replacement Parts Outlet Dip Tube, 5 L vessel 1 6977 Outlet Dip Tube, 10 L vessel 1 6978 Outlet Dip Tube, 20 L vessel 1 6979 Plug, 1/4 in. NPT, stainless steel 2 YY1301009 Street Elbow, 1/4 in. NPTF to M 1 XX6700104 Vessel Cover, cam-lock 1 6976 O-ring, fluoroelastomer 1 XX6700059

For more information visit: www.merckmillipore.com/LabHardware

204 www.merckmillipore.com Analytical Prepare 205

valve

volume outlet

ball to

XX1100000 SX0004700 hold-up valves

Delrin® holder

ball use

filter to

tubing;

Qty/Pk1 Catalogue No. 1 outlet

minimizes

prior

relief, pressure

Swinnex®

design

oduct loss holder effluent

er connector, filter

and pr filt polyethylene

• Attach Applications Vial of Liquid, Filling Small Volumes Dispensing Features& Benefits • Vertical tubing

including

O-rings;

NPTF

not

with filter

Clear polycarbonate cylinder; white polypropylene end caps; polypropylene white cylinder; Clear polycarbonate silicone 1/4 in. 6.9 9 41.9; 1.1 vials

Swinnex®

A filling

ached to the outlet ball valve ball valve outlet the to ached filtration.

solutions.

Holder

particle-free

Filter

for

ophthalmic

point-of-use

Pressure Vessel, 600 mL Vessel, Pressure Accessories Swinnex® Ordering Information Ordering Description Materials Specifications Fittings bar Pressure, Maximum Inlet kg Weight, Diameter, cm Diameter, Height, cm xtemporaneous drug, allergenic extracts, allergenic drug, xtemporaneous www.merckmillipore.com For more information visit: www.merckmillipore.com/LabHardware visit: For information more e and holder can be att For dispensing volumes up to 600 mL 600 up to volumes For dispensing was This vertical-style vessel pressure designed for Pressure Vessel, 600 mL Vessel, Pressure EZ-Fit® Filter Holder Manifolds Simultaneous filtration of three or six test samples compatible with disposable filtration devices, • Quick-fit connections for the vacuum tubing, as well as stainless steel and glass funnels. the filtration heads and the on/off valves • Low height for ease of use in laminar flow hoods Features & Benefits • Different filtration heads for both reusable and disposable filtration devices Applications • Each component can be removed by hand Universal Laboratory Analysis, Water Testing and autoclaved Analysis, Microbiological Analysis, Particle • Easy access to all inner parts for efficient Contamination Analysis cleaning

The EZ-Fit® Manifold is designed to increase laboratory workflow efficiency and make Specifications laboratory filtration easier. It features quick-fit Materials Aluminum connections for assembly and disassembly Handles, valve (trigger and knob) 316L stainless steel without tools and a low profile to increase Connectors, pipe and valve body EPDM operator comfort. Plus, all internal areas are Connectors, seals and valve seals Silicone accessible for easy cleaning, facilitating biofilm Filtration O-ring 168 W x 433 L x 117 H prevention. Different filtration heads, all with Dimensions, mm* 2.9 quick-fit connections, make the manifold Weight, kg* Tubing Connection Hose barb for 10 mm tubing (NPS 3/8 or DN10) Autoclaving Conditions 121 °C for 30 min *For a 3-place manifold (cat. no. EZFITMIC03) Analytical

Ordering Information Description Qty/Pk Catalogue No.

Prepare EZ-Fit® Manifold, 1-place, stainless steel 1 EZFITL0W01 EZ-Fit® Manifold, 3-place, stainless steel 1 EZFITL0W03 EZ-Fit® Manifold, 6-place, stainless steel 1 EZFITL0W06 Replacement Parts EZ-Fit® Manifold Quick Connection, 1 plug and 1 connector 1 EZFITQUICKC EZ-Fit® Manifold Check Valve 3 EZFITBACKF EZ-Fit® Manifold O-ring Kit 3 EZFITGASK3 EZ-Fit® Manifold Complete Valve 1 EZFITVALV1 EZ-Fit® Manifold Removable Porous Filter Support, stainless steel 3 EZFITFRIT3 EZ-Fit® Manifold Maintenance Kit including lubricant and brush 1 EZFITMAKIT No.8 Blue Stopper (9.5 mm - 3/8 in.), silicone 5 XX2004718 Accessories Vacuum Filtering Flask, 1 L 1 XX1004705 Filter Forceps, blunt end, stainless steel 3 XX6200006P Hose Adapter, 1/4 in. NPTF to 5/16 in. ID, polypropylene 2 XX4304704 Millex®-FG Filter Unit, 0.2 µm, hydrophobic PTFE, 25 mm, PVC, ethylene 50 SLFG025LS oxide-sterilized High Output Pump, 115 V/60 Hz 1 WP6211560 High Output Pump, 220 V/50 Hz 1 WP6222050 High Output Pump, 100 V/50–60 Hz 1 WP6210060 EZ-Stream® Vacuum Pump 1 EZSTREAM1

For more information visit: www.merckmillipore.com/LabHardware

206 www.merckmillipore.com Analytical Sample Preparation

Collect Prepare Test

Whether you are performing chromatographic analysis or measuring particulate page 125 page 147 contamination, our columns, thin layer chromatography plates and test kits help deliver reproducible data in compliance with regulatory requirements. Chromolith® HPLC Columns Speed and performance in monolithic form Chromolith® HPLC columns provide excellent Features & Benefits separations in a fraction of the time required • Increased analysis speed by a standard particulate column, because • Improved HPLC system security Chromolith® columns are made from highly • Column length no longer limited by porous, monolithic rods of silica with a pressure revolutionary, bimodal pore structure. Instead • Cost savings from increased sample of being packed with small particles, each of throughput can justify the expense of a these monolithic columns consists of a single method revalidation within one month piece of high-purity, polymeric silica gel.

Applications Liquid Chromatography

Ordering Information

Description Qty/Pk Catalogue No. Chromolith® HighResolution RP-18 endcapped 100-4.6 mm 1 pc 1.52022.0001 Chromolith® Performance RP-18 endcapped 100-4.6 mm 1 pc 1.02129.0001 Chromolith® Performance RP-18 endcapped 100-3 mm 1 pc 1.52001.0001 Chromolith® Performance RP-18 endcapped 100-2 mm 1 pc 1.52006.0001

Chromolith® Performance NH2 100-4.6 mm 1 pc 1.52028.0001 Chromolith® Performance Si 100-4.6 mm 1 pc 1.51465.0001 Chromolith® Performance CN 100-4.6 mm 1 pc 1.52048.0001

Analytical Chromolith® Performance Phenyl 100-4.6 mm 1 pc 1.52058.0001 Chromolith® Performance Diol 100-4.6 mm 1 pc 1.53172.0001 Test For more information visit: www.merckmillipore.com/chromolith

208 www.merckmillipore.com Analytical Test 209

peak

reproducibility

excellent

properties

and

1 pc 1.51453.0001 1 pc 1.50144.0001 1 pc 1.50169.0001 1 pc 1.50648.0001 1 pc 1.50398.0001 1 pc 1.50356.0001 1 pc 1.50470.0001 Qty/Pk Catalogue No. 1 pc 1.50247.0001 efficiency

lifetime

batch-to-batch

performance column

chromatographic separation

try symme Applications Chromatography Liquid • Excellent THe Purospher® STAR RP-8 endcapped RP-8 STAR THe Purospher® polar of separations column is suitable for of positionally or separation compounds compounds. isomeric • Outstanding • Balanced • Extended Features & Benefits • Enhanced 150-4.6 mm 2 Purospher® STAR NH STAR Purospher® Purospher® STAR RP-8 endcapped 150-4.6 mm endcapped RP-8 STAR Purospher® Purospher® RP-18 250-4 mm RP-18 Purospher® Purospher® RP-18 endcapped 250-4 mm RP-18 Purospher® Purospher® STAR RP-18 endcapped UHPLC 100-4.6 mm UHPLC endcapped RP-18 STAR Purospher® Purospher® STAR RP-18 endcapped 100-3 mm endcapped RP-18 STAR Purospher® Purospher® STAR Si 150-4.6 mm STAR Purospher® Ordering Information Ordering Description 150-4.6 mm endcapped RP-18 STAR Purospher® For more information visit: www.merckmillipore.com/purospher visit: For information more www.merckmillipore.com The Purospher® STAR RP-18 endcapped RP-18 STAR The Purospher® of separation tailing-free the column allows compounds acidic, basic or chelating neutral, column. UHPLC as a special and is available The Purospher® RP-18 HC column is not HC column is not RP-18 The Purospher® of separations endcapped and is suitable for non-basic compounds. polar, The Purospher® RP-18 endcapped column is endcapped column RP-18 The Purospher® samples of compley separations suitable for eluents. simple with The all-around, high-performance solution The all-around, is polar column RP-18 The Purospher® separations and suitableendcapped for compounds basic or chelating of strong and separations (no acidic compounds) high a with compounds of hydrophilic mobile phase. in the of water percentage Purospher® Columns Purospher® LiChrospher® Columns Silica carrier for consistently top-rate results Reliable, versatile, traditionally produced Features & Benefits spherical silica carriers, LiChrospher® columns • Well-balanced pressure / separation are available with a number of different performance ratio modifications. The polar-modified phases, • Widely used for a broad range of applications LiChrospher® CN, LiChrospher® NH2 and LiChrospher® DIOL columns , as well as unmodified LiChrospher® Si columns are Applications best for normal-phase HPLC. Furthermore, Liquid Chromatography LiChrospher® PAH columns are highly efficient and selective for the separation of polycyclic aromatic hydrocarbons (PAH), and LiChrospher® WP 300 RP-18 columns are suitable for the separation of peptides and low molecular weight proteins.

Ordering Information

Description Qty/Pk Catalogue No. LiChrospher® 100 RP-18 endcapped 150-4.6 mm 1 pc 1.50604.0001 LiChrospher® 100 RP-18 150-4.6 mm 1 pc 1.50601.0001 LiChrospher® 100 RP-8 endcapped 150-4.6 mm 1 pc 1.50638.0001 LiChrospher® 100 RP-8 150-4.6 mm 1 pc 1.50635.0001 LiChrospher® 60 RP-select B 150-4.6 mm 1 pc 1.50641.0001 Analytical LiChrospher® 100 CN 250-4 mm 1 pc 1.50892.0001 LiChrospher® 100 Diol 250-4 mm 1 pc 1.50836.0001

LiChrospher® 100 NH2 250-4 mm 1 pc 1.50834.0001 Test LiChrospher® 60 Si 250-4 mm 1 pc 1.50830.0001

For more information visit: www.merckmillipore.com/lichrospher

210 www.merckmillipore.com Analytical Test 211 1 pc 1.50657.0001 1 pc 1.50660.0001 1 pc 1.50462.0001 1 pc 1.50464.0001 1 pc 1.50447.0001 1 pc 1.50453.0001 Qty/Pk Catalogue No. Qty/Pk Catalogue No. 25 plates 1.05715.0001 50 plates 1.05642.0001 25 plates 1.15389.0001 50 plates 1.16225.0001 25 plates 1.05586.0001 mm mm mm mm

mm mm

, two- 100-2.1 100-4.6 100-2.1 100-4.6

and 100-2.1 100-4.6

sample

samples

s 20x20 cm by Column Column Column Column 254

Column Column

LC-MS

reversed-phase many

simplified

10x20 cm

hydrophilic HPLC HPLC HPLC HPLC

to HPLC HPLC 254 of results

s 20x20 cm

with

254 of PEEK PEEK

PEEK PEEK 20x10 cm

PEEK PEEK ensure

254 25x25 cm 254 analysis

selectivity

plates

pounds separation stability

combination

ZIC®-cHILIC ZIC®-cHILIC ZIC®-pHILIC ZIC®-pHILIC ZIC®-HILIC ZIC®-HILIC

visualization

ivatization eparation polar com columns Optimal pr der under identical conditions using easy dimensional separation SeQuant® SeQuant® SeQuant® SeQuant® SeQuant® Description SeQuant® Ordering Information Ordering HPTLC Silica Gel 60 F HPTLC TLC Silica Gel 60 RP-18 F Silica Gel 60 RP-18 TLC HPTLC Silica Gel 60 RP-18 F Silica Gel 60 RP-18 HPTLC HPTLC LiChrospher® Silica Gel 60 F LiChrospher® HPTLC Ordering Information Ordering Description Silica Gel 60 F TLC • Excellent Features& Benefits • Improved • Orthogonal • Applications Chromatography Liquid Applications Chromatography Thin Layer • Direct For more information visit: www.merckmillipore.com/hilic visit: For information more • Simultaneous Features & Benefits • Disposable For more information visit: www.merckmillipore.com/tlc visit: For information more

using

LC-MS

separate

benefit

Enhanced

difficult

are

additional columns.

that

y hese columns. eversed-phase HPLC columns is ensured columns is ensured HPLC eversed-phase TLC/HPTLC Plates TLC/HPTLC quality pioneer in TLC the from Unique www.merckmillipore.com Thin layer chromatography (TLC) is a simple, is a simple, (TLC) chromatography Thin layer for tool separation fast versatile and highly analyses. and quantitative qualitative both virtually resolve can be used to The technique all classes of substances, including pesticides, lipids, nucleotides, alkaloids, steroids, fatty carbohydrates, acids glycosides, manual the Apart from others. and many can technique the of classical TLC, method high as in instrumental also be automated chromatography layer thin performance it can be easily Furthermore, (HPTLC). PLC. scale for preparative extended to sensitivit t r zwitterionic high-performance, the by stationary columns. phase in these of compounds separation Straightforward acids and bases, anions and cations, as such complexes, metal metabolites, carbohydrates, and digests protein amino acids, peptides, achieved be can therefore oligonucleotides a selectivitywith complementary to reversed-phase Ideal for polar and hydrophilic compounds and hydrophilic polar Ideal for of all types separation for The ideal choice is compounds hydrophilic of polar and and ZIC®-pHILIC SeQuant® ZIC®-HILIC of retention columns. Reproducible HPLC compounds SeQuant® ZIC®-HILIC Columns ZIC®-HILIC SeQuant® Fluid Sampling Kit Field-based, liquid analysis with 37 mm monitors The Fluid Sampling Kit is a complete Features & Benefits solution for field-based fluid collection and • Sturdy carrying case and stainless steel contamination analysis. The stainless steel sampling assembly provide years of in-field sampler assembly plugs into a quick-release service valve installed in the system line, which • Fluid Sampling Kit includes 12 Tenite® allows a measured quantity of liquid to pass contamination monitors through the monitor. A valved syringe serves as a pump to remove residual liquid from the Applications monitor. Kit is compatible with our 37 mm In-Field Collection and Analysis of Jet Fuel, contamination monitors and matched-weight Turbine Lubricants, Water and Other Liquids monitors.

Specifications Materials Stainless steel with stainless-clad PTFE hose; Tenite® monitors Fittings Quick-release valve and plug with 1/8” NPTF thread and fluoroelastomer seals; matching nipple on sampling hose Maximum Pressure, bar (psi) 6.9 (100) Dimensions Length, cm 36.8 Height, cm 12.5 Width, cm 23.5 Shipping Weight, kg (lb) 4.5 (9.9) Analytical Warning: For filtering flammables with the Fluid Sampling Kit, properly ground the unit and secure all grounding connections. Test

Ordering Information Description Qty/Pk Catalogue No. Fluid Sampling Kit 1 XX6403730* Common Components Fluid Contamination Analysis Monitor 50 MAWP037P0 Fluid Contamination Analysis Monitor, matched-weight membrane 50 MAWP037PM PTFE Tape 12.5 mm x 6.6 m roll 1 TP0001326 Filter Forceps, blunt end, stainless steel 3 XX6200006P Hand Vacuum Pump, stainless steel 1 XX6200035 Tubing, 3/16 in. I.D., Tygon 1 XX6403780 Hose Connector, 1/8 in. NPTF quick-release valve 1 XX6403735 Sampler Valve Hose Assembly 1 XX6403708 Remote Sampling Assembly 1 XX6403705 Accessory ASTM® Color Standards 1 ASTM03701 *The Fluid Sampling Kit (cat. no. XX6403730) contains 12 monitors. The reorder part number (cat. no. MAWP037P0) includes 50 monitors.

For more information visit: www.merckmillipore.com/ParticleMonitoring

212 www.merckmillipore.com Analytical Test 213

for

115

Water,

of HABG04700 HAWP0470M AAWP0470M XX7104711K

materials either

pack.

for per

100

Qty/Pk Catalogue No. 1 XX7104712 1 XX2504755 11 WP6111560 100* WP6122050 HAWP04700 100* HAWG04700 100* 50 pairs 50 pairs 13 XX1004705 1 XX6200006P 100* XX6602500 PD1504700 1 1 XX1004730 is

pump

Monitoring

expendable

current

quantity

vacuum

enough electric a

white

V reorder white

oximately 600 tests oximately The 0.45 µm, 0.45 µm, 0.45 µm,

Contamination

220

aulic Fluid, Fuel Liquids and Related and Related Liquids aulic Fluid, Fuel

47 mm,

appr or 47 mm, type.

• Includes Applications Fluid Hydr Fluids Features& Benefits • Contains filter

esters,

Hydrophilic, Hydrophilic, Hydrophilic,

47 mm

membrane cellulose esters, esters, esters,

cellulose

Screen, each

tests:

mixed of

complete mixed

federal cellulose cellulose cellulose Steel a

200 Hz Hz

and and

25 mm 0.45 µm, 0.8 µm, Kit mixed mixed mixed

V/60 V/50

230 V/50 Hz 115 V/60 Hz L gravimetric

Stainless 1

latex

115 220 Kit, Kit, for

Pairs, Pairs, fluids)

PetriSlides with

Flask, dware and consumables to to and consumables dware

Dispenser, 23 in.,

Pump, Pump, Membrane, Membrane, Membrane, 400 plain gridded

gridded

available

Holder

Duty Duty Contamination (hydraulic

Filtering

white, white, black,

Filtering 1/4 in.

contains

Contamination Contamination filters Filter

kit

Matched-Weight Matched-Weight Fluid

acuum ecommended Accessories R Other 47 mm, *The Solvent Chemical 47 mm, MF-Millipore™ 47 mm, MF-Millipore™ MF Membrane Filters Membrane MF-Millipore™ Components Glass Description Fluids Ordering Information Ordering Filter Forceps, blunt end, stainless Forceps, Filter steel Tubing, Contamination Analysis for PetriSlides Chemical Fluids MF V tandard 791a (lubricants, liquid fuels, and liquid 791a (lubricants, tandard www.merckmillipore.com For more information visit: www.merckmillipore.com/ParticleMonitoring visit: For information more s products). related Start-up kit for fluid contamination analysis for kit Start-up The har collection of contamination for lab your equip properly standard to analysis. kit conforms The as such procedures contamination test ARP-598 Fluids ContaminationFluids Kit Patch Test Kit In-field contamination analysis The Patch Test Kit is a complete solution Features & Benefits for field-based collection and analysis of • Contains enough expendable materials for hydrocarbon-based hydraulic fluids, bulk 100 tests chemicals, boiler water, and lubricating oils. • Contained within a lightweight, It allows the detection of significant changes easy-to-carry case in cleanliness through dependable, sensitive, • Filter color rating and particle assessment colorimetric-based analysis. scales correspond to recognized standard contamination levels

Applications In-Field Contamination Analysis of Hydrocarbon-Based Hydraulic Fluids, Water, Lubricating Oils and Related Products

Specifications Materials Stainless steel/aluminum filter holder assembly; PVC/ polyethylene sample collection, solvent dispensing bottles Shipping Weight, kg (lb) 4.5 (9.9)

Ordering Information Analytical Description Qty/Pk Catalogue No. Patch Test Kit 1 XX6504730 Components

Test Patch Test Filter Holder Assembly 1 XX6300120 Hand Vacuum Pump, stainless steel 1 XX6200035 Tubing for Vacuum, 1/8 in., fluoroelastomer 1 XX6504710 Solvent Dispensing Bottle 1 XX6504704 Swinnex® 25 mm with back-pressure screen 1 XX6504707 PVC Bottle, 120 mL 1 XX6504709 Filter Forceps, blunt end, stainless steel 3 XX6200006P MF-Millipore™ Membrane, 5.0 µm, 47 mm, for test 100* SMWP04700 MF-Millipore™ Membrane, 5.0 µm, 25 mm, for solvent (25 included) 100* SMWP02500 PetriSlides for Contamination Analysis 100* PD1504700 Naval Fluid Color Guide 1 XX6504713 *Reorder quantity Note: Kit also includes operating instructions. Replacement Parts Stainless Steel Holder Support with base 1 XX6504708 Funnel Locking Ring 1 XX2004701 Gasket, PTFE 25 XX2004703 Locking Ring Gasket, PTFE 5 XX4004714 O-ring (2-233), Buna-N 10 XX6300123

For more information visit: www.merckmillipore.com/ParticleMonitoring

214 www.merckmillipore.com Product Highlight

High Quality Water Can Improve UHPLC Performance

Contaminating solutes may contribute to baseline variability and poor chromatographic performance when bottled water instead of freshly-delivered water is used to prepare the mobile phase (Figure 1). Use Milli-Q® water purification systems to ensure that your mobile phases are free of organic contaminants, for the best, most reproducible chromatographic results. Especially when fitted with a 0.2 μm final filter, Milli-Q® systems are the ideal Analytical water source for UHPLC, LC-MS, and other ultrasensitive analytical applications. Test

The effects of two different sources of water on chromatographic performance

A. HPLC-grade Bottled Water B. Freshly Produced Ultrapure Water

0.015 0.015

0.010 0.010 inj #770 inj #290, 530, 214 inj #1310 214 1010, 1310 AU 0.005 inj #770 AU 0.005 inj #50 inj #1010 inj #530

inj #290 0

inj #50 -0.005 -0.005 0 4 6 8 10 12 14 16 18 0 4 6 8 10 12 14 16 18 Time (Minutes) Time (Minutes)

Figure 1. Two different sources of water used as solvent in the gradient elution of repeated injections of a drug mixture affect chromatographic performance over time. The solvents being compared are (A) commercially available HPLC-grade bottled water without total oxidizable carbon (TOC) specifications and (B) freshly delivered ultrapure water with a TOC level of ≤ 5 ppb. HPLC-grade acetonitrile was used as the organic solvent. Elution order: 1–acetaminophen, 2–acetazolamide, 3–phenobarbital, 4–carbamaze- pine, 5–phenytoin, 6–secobarbital, 7–nabumetone. www.merckmillipore.com 215 Product Highlight

Laboratory water systems for UHPLC

Milli-Q® Integral system The unique range of compact Milli-Q® Integral water systems uses advanced technology to provide both pure and ultrapure water from tap water, all in a single unit. Dual points of delivery (PODs) save space and increase convenience. Equipped with online TOC and resistivity monitors, the Milli-Q® Integral system gives the user total control over water quality and quantity at the point of delivery.

Description Cat. No. Milli-Q® Integral 15 Pure (15 L/hour) and Ultrapure (2 L/min) Water Production Unit with ZRXQ015T0* built-in resistivity and TOC meter designed for USP suitability test Analytical

Test Milli-Q® Advantage A10 system Using an optimized purification sequence, the Milli-Q® Advantage A10 water purification system converts pure water to ultrapure water, then delivers it to a POD, which provides final polishing adapted to your specific needs.

Description Cat. No. Milli-Q® Advantage A10 Ultrapure Water Purification System Z00Q0V0WW*

Bio-Pak® polisher: designed for advanced life science techniques The BioPak® unit is a disposable ultrafiltration cartridge designed for installation at the outlet of Type 1 water purification systems and is typically used in cell culture, biochemistry or molecular biology applications

Description Cat. No. Bio-Pak® Ultrafiltration Cartridge CDUFBI001

*Contact your local sales representative for a country-specific part number.

216 www.merckmillipore.com Appendix

Multiwell Plate Selection Guide page 218 Tables of Chemical Compatibility page 220 Index by Catalogue Number page 235 Index by Product/Application page 246 MultiScreen® Selection Guide

Membrane Number of Application Type(s) Pore Size (µm) Samples Sample Volume (mL) Plate Type(s) Page

Drug solubility studies Polycarbonate 0.4 96 0.25 MultiScreen®HTS-PCF Filter Plates 167 for Solubility Assays PVDF 0.45, 0.22 96, 384 0.25, 0.1 MultiScreen® Plate with 168 Durapore® Membrane PTFE 0.4 96 0.25 MultiScreen Solvinert™ and 167 Deepwell Solvinert™ Plates PVDF 0.45 2.0 MultiScreen® High Volume plate 168 with Durapore® Membrane Permeability studies/ Polycarbonate 0.4 96 0.25 MultiScreen® Permeability Plate 167 PAMPA assay PVDF 0.45 96 0.25 MultiScreen®-IP Plate for PAMPA 35, 36 Cell-based drug Polycarbonate 0.4 96 0.25 MultiScreen® Caco-2 Assay System 24, 167 permeability studies PET 1 96 0.25 MultiScreen® Caco-2 Assay System 24 Total drug analysis PTFE 0.4 96 0.25 MultiScreen Solvinert™ and 167 Deepwell Solvinert™ Plates Clarification of cellular Glass Fiber 1.0, 1.2 96 0.25 MultiScreen®-FB or -FC Plate 169 debris or particulates Glass Fiber 1.2 96 2.0 MultiScreen® High Volume Plate 169 with FC Dye terminator removal PVDF 0.45 96 0.25 MultiScreen® Plate with 168 (with Sephadex® and Durapore® Membrane column loader) PCR cleanup Specialty NA 96 0.25, 0.1 MultiScreen®-PCR and -µPCR Plates 52, 53 Lysate clearing Specialty NA 96 0.25 MultiScreen®-NA Plate 55 DNA-binding proteins PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36

Appendix Immobilon®-P membrane Protein binding PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36 Immobilon®-P membrane Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 Esters Cloned receptor- PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 binding studies with Durapore® Membrane PVDF 0.45 96 2.0 MultiScreen® High Volume Plate 168 with Durapore® Membrane Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 Glass Fiber 1.2 96 2.0 MultiScreen® High Volume Plate 169 with FC Protein kinase/ PVDF 0.45, 0.22 96, 384 0.25, 0.1 MultiScreen® Plate with 168 phosphatase assays Durapore® Membrane Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 ELISA Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 Esters Membrane PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36 absorbance assays Immobilon®-P membrane Protein kinase assays Negatively NA 96 0.25 MultiScreen®-PH Plate 165 Charged Phosphocellulose Reverse transcriptase Positively NA 96 0.25 MultiScreen®-DE Plate See web assays Charged DEAE Enzyme assays with Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 precipitation Virus concentration Regenerated 10,000 MWCO 96 0.25 MultiScreen® Plate with See web Cellulose Ultracel®-10 Membrane Ultrafiltration Membrane

218 www.merckmillipore.com MultiScreen® Selection Guide

Membrane Number of Membrane Number of Application Type(s) Pore Size (µm) Samples Sample Volume (mL) Plate Type(s) Page Application Type(s) Pore Size (µm) Samples Sample Volume (mL) Plate Type(s) Page

Drug solubility studies Polycarbonate 0.4 96 0.25 MultiScreen®HTS-PCF Filter Plates 167 Bacteria PVDF 0.22, 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 for Solubility Assays with Durapore® Membrane PVDF 0.45, 0.22 96, 384 0.25, 0.1 MultiScreen® Plate with 168 Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 Durapore® Membrane Esters PTFE 0.4 96 0.25 MultiScreen Solvinert™ and 167 Cell fragment/ Glass Fiber 1.0, 1.2 96 0.1 MultiScreen® Harvest Plate 169 Deepwell Solvinert™ Plates receptor-binding assays PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 PVDF 0.45 2.0 MultiScreen® High Volume plate 168 (radiometric) with Durapore® Membrane with Durapore® Membrane Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 Permeability studies/ Polycarbonate 0.4 96 0.25 MultiScreen® Permeability Plate 167 Calcium uptake PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 PAMPA assay PVDF 0.45 96 0.25 MultiScreen®-IP Plate for PAMPA 35, 36 measurements with Durapore® Membrane Cell-based drug Polycarbonate 0.4 96 0.25 MultiScreen® Caco-2 Assay System 24, 167 Studies using cell Polycarbonate 0.45, 1.0 96 0.25 Millicell® 96- or 24-Well Insert 24 permeability studies PET 1 96 0.25 MultiScreen® Caco-2 Assay System 24 monolayers Plates for Transporter Assays Total drug analysis PTFE 0.4 96 0.25 MultiScreen Solvinert™ and 167 Elispot assays to Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 Deepwell Solvinert™ Plates determine immune Esters response Clarification of cellular Glass Fiber 1.0, 1.2 96 0.25 MultiScreen®-FB or -FC Plate 169 PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36 Immobilon®-P Membrane debris or particulates Glass Fiber 1.2 96 2.0 MultiScreen® High Volume Plate 169 with FC Migration, invasion, Polycarbonate 8.0, 5.0, 3.0 96 0.25 MultiScreen®-MIC Plate 34 chemotaxis Dye terminator removal PVDF 0.45 96 0.25 MultiScreen® Plate with 168 (with Sephadex® and Durapore® Membrane Cell proliferation and Glass Fiber 1.0, 1.2 96 0.1 MultiScreen® Harvest Plate 169 column loader) whole cell receptor PVDF 0.22, 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 binding assays Appendix PCR cleanup Specialty NA 96 0.25, 0.1 MultiScreen®-PCR and -µPCR Plates 52, 53 with Durapore® Membrane (radiometric) Lysate clearing Specialty NA 96 0.25 MultiScreen®-NA Plate 55 Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 DNA-binding proteins PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36 Viral infection studies PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 Immobilon®-P membrane with Durapore® Membrane Protein binding PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36 Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 Immobilon®-P membrane Esters Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 Polycarbonate 0.45, 1.0 96 0.25 MultiScreen®-96 or 24 Assay 24 Esters System Cloned receptor- PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 Immune response PVDF 96 0.25 MultiScreen® Plate 168 binding studies with Durapore® Membrane assays with Durapore® Membrane PVDF 0.45 96 2.0 MultiScreen® High Volume Plate 168 Chromatography - PVDF 0.45, 1.2 96 0.25 MultiScreen® Plate 168 with Durapore® Membrane affinity capture with Durapore® Membrane Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 Chromatography - PVDF 0.45, 1.2 96 0.25 MultiScreen® Plate 168 gel permeation with Durapore® Membrane Glass Fiber 1.2 96 2.0 MultiScreen® High Volume Plate 169 with FC Chromatography - ion PVDF 0.45, 1.2 96 0.25 MultiScreen® Plate 168 exchange (resins) with Durapore® Membrane Protein kinase/ PVDF 0.45, 0.22 96, 384 0.25, 0.1 MultiScreen® Plate with 168 phosphatase assays Durapore® Membrane Environmental samples PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 with Durapore® Membrane Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 Whole tissue Glass Fiber 0.45, 1.2 96 0.1 MultiScreen® Harvest Plate 169 ELISA Mixed Cellulose 0.45 96 0.25 MultiScreen®-HA Plate 35, 36 radiometric receptor Esters PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 binding assays with Durapore® Membrane Membrane PVDF 0.45 96 0.25 MultiScreen® Plate with 35, 36 absorbance assays Immobilon®-P membrane Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 Protein kinase assays Negatively NA 96 0.25 MultiScreen®-PH Plate 165 Neurotransmitter PVDF 0.45 96, 384 0.25, 0.1 MultiScreen® Plate 168 Charged release from brain with Durapore® Membrane Phosphocellulose tissue Reverse transcriptase Positively NA 96 0.25 MultiScreen®-DE Plate See web Whole organisim Nylon Mesh Variable 96 0.25 MultiScreen®-MESH Plate 38 assays Charged DEAE cytotoxicity, paralysis, and morbidity Enzyme assays with Glass Fiber 1.0, 1.2 96, 384 0.25, 0.1 MultiScreen®-FB or -FC Plate 169 precipitation Luminex® bead-based PVDF 0.45, 1.2 96 0.25 MultiScreen® Plate 168 assays with Durapore® Membrane Virus concentration Regenerated 10,000 MWCO 96 0.25 MultiScreen® Plate with See web Cellulose Ultracel®-10 Membrane Ultrafiltration Membrane

www.merckmillipore.com 219 Chemical Compatibility of Filter Components Compatibility of materials used in the construction of Merck Millipore products

The tables on the following pages contain recommendations based solely upon ratings C aUTIon found in several published solubility and compatibility tables. A listing of sources Please note the following: appears below. The tables do not contain 1. These recommendations assume pure solutions at room temperature and pressure results from any actual usage experiments without applied stresses. Time of exposure is not considered. These are critical conducted at Merck Millipore. assumptions as polymer properties are strongly affected by environmental conditions, time, the presence of external stress and the presence of additives. It is not safe to Ratings for this table were made assume that property changes are linearly related to changing temperature. A 10 °C increase in temperature, for example, may place the test conditions closer to the glass on the following basis: transition of the polymer, thus allowing greater penetration of solvent molecules. R = Recommended . Chosen for only those This has a plasticizing effect, further lowering the glass transition and resulting in a polymer-solvent combinations that modulus drop of up to three orders of magnitude. The glass transition of nylons, for showed top ratings in the published example, has been shown to range from below -50 °C to +70 °C depending upon their compatibility tables. moisture content. GR = Generally Recommended. Where no polymer-solvent specific information 2. These recommendations assume that each polymer category has a uniform chemistry, was available, general compatibility molecular weight distribution and thermomechanical history. This assumption will with a solvent type was stated; e.g., never be true and, in some cases, variation has a distinct influence on compatibility. PES is compatible with alcohols. For example, solvent compatibility of cellulose esters is strongly dependent upon their Appendix LTD = Limited. Chosen for those polymer- degree of substitution (acetylation/nitration). Crystalline morphology and degree solvent combinations that showed of crystallinity influences compatibility of semi-crystalline polymers and can vary both top and less-than-top ratings significantly. Polyethyleneterephthalate, for example, can be quenched to obtained in published compatibility tables samples with almost no crystallinity or annealed to obtain samples with >50% where exposure times of less than crystallinity. The response time of these two polyesters, although chemically identical, 24 hours at room temperature are will be quite different. The effect of molecular weight distribution and degree of recommended. Component materials branching on solvent compatibility can be seen by comparing the solvent compatibility may stress crack or swell affecting of LDPE, LLDPE, HDPE and UPE. Such specific information concerning polymers filtration efficiency. evaluated does not accompany published compatibility tables. NR = Not Recommended. Chosen for those 3. The definition of solvent compatibility for Merck Millipore products differs from that polymer-solvent combinations that used in determining the ratings given in published compatibility tables. Such tables showed less-than-top ratings in the are generally concerned with chemical attack and significant losses in strength and/ published compatibility tables. or dimensional changes. A top designation, for example, might be designated for solvent-polymer combinations with <10% swelling, which is high. Other compatibility tables may make recommendations based upon dimensional change as a function of References used in developing this table: time. This is difficult to relate to a membrane that may respond almost immediately to 1. Polymer Handbook, ed III., Section VII p. 379-402 lists solvents and nonsolvents for a variety of polymers immersion in solvent. In addition, solvent-membrane compatibility requires additional 2. Organic Polymer Chemistry, 2nd edition, K.J.Saunders, Chapman consideration of filtration-specific factors. None of these published compatibility & Hall, 1988. guides, for example, monitors the solvent’s ability to wet a membrane or increase 3. Appropriate sections in Encyclopedia of Polymer Science and Engineering extractables. 4. Chemical Resistance Guide for Elastomers, Compass Publications. Kenneth M.Pruett, 1988. 4. This table does not consider solvent safety issues. 5. Compass Corrosion Guide II, Compass Publications. Kenneth M.Pruett, 1983. 6. http://www.pspglobal.com/nfvitongrades.html (Compatibility of vinylidiene fluoride-hexafluoropropylene copolymer) 7. http://www.pspglobal.com/nfmaterials.html (Compatibility of 9. http://www.customadvanced.com/chemical-resistance-chart.html 12. Bottino, Capannelli, Munari & Turturro, J.Polymer Science: O-ring materials) 10. Merck Index, 10th edition Polymer Physics, Vol 26, 1988, p. 785-94. 8. http://www.ab.ust.hk/sepo/tips/ch/ch004.htm (General polymer 11. Chapiro, Mankowski & Schmitt, J.Polymer Science: Chemistry 13. CRC Handbook of Polymer-Liquid Interaction Parameters and compatibility) edition, Vol.20, 1982, p. 1791-1796 Solubility Parameters, Allan F.M. Barton, CRC Press, 1990.

220 www.merckmillipore.com Chemical Compatibility of Filter Components

SAN (styrene-acrylonitrile Appendix ND ND ND ND ND ND ND GR GNR ND ND polymer) PC (polycarbonate) R NR NR TST NR R NR R NR NR GR PET (polyethylene terephthalate) NR R ND ND ND R TST R R NR R EASTAR® (copolyester) ND NR ND ND ND ND ND ND ND NR ND Filter Materials PP (polypropylene) R R NR ND TST R TST R NR R R PVC (polyvinyl chloride) R NR NR ND TST R NR R NR NR R PC (polycarbonate) R GNR NR R TST R R R NR TST GR PTFE (polytetrafluoroethylene) R R R TST GR GR R R R R GR PVDF (polyvinylidene fluoride) R NR LTD TST R NR R R R R TST MCE (mixed cellulose esters) NR NR NR TST NR NR NR NR GR NR GR PES (polyether sulfone) R GNR NR ND NR ND GR GR NR ND GR NYL (nylon) NR R R TST TST R TST TST R TST R O-ring Materials EPR (ethylene propylene rubber) TST R R ND R R R R NR R R Buna-N (nitrile rubber) NR NR NR ND NR R NR R NR R TST Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene NR NR NR ND R R NR R R R R copolymer) Silicone (silicone) R R NR ND R R NR NR NR R R Filter Holder Material 316 SS (stainless steel) RRR LTD LTD RRRRRR

The following descriptions are abbreviated. Please see the beginning of this section for complete information. R = Recommended; GR = Generally Recommended LTD = Limited Recommendation; NR = Not Recommended; GNR = Generally Not Recommended; TST = Testing Recommended; ND = No Data Presently Available www.merckmillipore.com 221 Chemical Compatibility of Filter Components

Chemical Compatibility of Merck Millipore Products This table shows the chemical compatibility of materials used in the fabrication of Merck Millipore devices and membranes. Please refer to the specification tables on product-specific pages for materials found in specific Merck Millipore devices. ), O Butyl Acetate ester Butyl Alcohol alcohol Tetrachloride Carbon HC, halogenated (Ethyl) Cellosolve ether glycol solution) CHAPS (aqueous surfactant/detergent Chloroform HC, halogenated Cyclohexanone ketone 0.2% Pyrocarbonate, Diethyl anhydride carboxylic (DMS Sulfoxide Dimethyl sulfoxide Dimethylacetamide amide Dimethylformamide amide Housing Materials HDPE (high density polyethylene) R R NR R TST LTD R ND R R R PP (polypropylene) TST R NR R ND NR NR ND R R R PS (polystyrene) NR R NR NR ND NR NR ND R NR NR PVC (polyvinyl chloride) NR R NR NR ND NR NR ND NR NR NR MMA (modified acrylic copolymer) GNR TST NR NR ND NR GNR ND NR ND ND ABS (acrylonitrile-butadiene- GNR GR ND ND ND ND TST ND NR NR NR styrene polymer) Appendix SAN (styrene-acrylonitrile ND GR ND ND ND ND ND ND ND ND ND polymer) PC (polycarbonate) NR R NR NR TST NR NR ND NR NR NR PET (polyethylene terephthalate) R R R ND ND R R ND ND ND NR EASTAR® (copolyester) ND ND ND R ND ND NR ND ND ND ND Filter Materials PP (polypropylene) TST R TST R ND TST R ND R R R PVC (polyvinyl chloride) NR R NR NR ND NR NR ND NR NR NR PC (polycarbonate) RR TST R TST NR TST ND NR TST NR PTFE (polytetrafluoroethylene) GR GR GR GR TST R R ND R GR GR PVDF (polyvinylidene fluoride) TST R R ND ND R NR TST NR NR NR MCE (mixed cellulose esters) NR R R NR ND R NR NR NR NR NR PES (polyether sulfone) GNR GR GNR GR ND GNR GNR ND NR NR ND NYL (nylon) RR TST R TST NR R ND R NR R O-ring Materials EPR (ethylene propylene rubber) R R NR R ND NR TST ND NR NR NR Buna-N (nitrile rubber) NR R NR NR ND NR NR ND NR NR NR Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene NR RR TST TST R NR ND NR NR NR copolymer) Silicone (silicone) NR R NR NR ND NR TST ND NR R R Filter Holder Material 316 SS (stainless steel) RRR LTD LTD R R R NR R R

222 www.merckmillipore.com Chemical Compatibility of Filter Components

Butyl Acetate ester Butyl Alcohol alcohol Tetrachloride Carbon HC, halogenated (Ethyl) Cellosolve ether glycol solution) CHAPS (aqueous surfactant/detergent Chloroform HC, halogenated Cyclohexanone ketone 0.2% Pyrocarbonate, Diethyl anhydride carboxylic (DMS O ), Sulfoxide Dimethyl sulfoxide Dimethylacetamide amide Dimethylformamide amide PS (polystyrene) NR NR NR TST ND NR NR NR NR R ND Housing Materials PVC (polyvinyl chloride) NR NR NR R TST TST TST NR NR R ND HDPE (high density polyethylene) R R NR R TST LTD R ND R R R MMA (modified acrylic copolymer) NR TST NR TST ND ND TST ND ND ND GR PP (polypropylene) TST R NR R ND NR NR ND R R R ABS (acrylonitrile-butadiene- ND ND GNR GR ND ND ND ND ND ND ND PS (polystyrene) NR R NR NR ND NR NR ND R NR NR styrene polymer)

PVC (polyvinyl chloride) NR R NR NR ND NR NR ND NR NR NR SAN (styrene-acrylonitrile Appendix NR ND ND GR ND ND ND ND ND ND ND MMA (modified acrylic copolymer) GNR TST NR NR ND NR GNR ND NR ND ND polymer) ABS (acrylonitrile-butadiene- PC (polycarbonate) NR NR NR TST R R R NR NR R ND GNR GR ND ND ND ND TST ND NR NR NR styrene polymer) PET (polyethylene terephthalate) GR NR ND R R R NR R R R ND SAN (styrene-acrylonitrile EASTAR® (copolyester) ND ND NR R ND ND ND ND ND ND ND ND GR ND ND ND ND ND ND ND ND ND polymer) Filter Materials PC (polycarbonate) NR R NR NR TST NR NR ND NR NR NR PP (polypropylene) R NR TST R R R R R NR R ND PET (polyethylene terephthalate) R R R ND ND R R ND ND ND NR PVC (polyvinyl chloride) NR NR NR R TST TST TST NR NR R ND EASTAR® (copolyester) ND ND ND R ND ND NR ND ND ND ND PC (polycarbonate) NR TST TST R R R R GR R R R Filter Materials PTFE (polytetrafluoroethylene) NR TST TST R R R R GR R R R PP (polypropylene) TST R TST R ND TST R ND R R R PVDF (polyvinylidene fluoride) R R R R R R R R R R ND PVC (polyvinyl chloride) NR R NR NR ND NR NR ND NR NR NR MCE (mixed cellulose esters) NR NR NR NR NR NR ND R R R ND PC (polycarbonate) RR TST R TST NR TST ND NR TST NR PES (polyether sulfone) ND ND GNR GR GR ND ND ND GR GR ND PTFE (polytetrafluoroethylene) GR GR GR GR TST R R ND R GR GR NYL (nylon) RRR TST R R NR R R R ND PVDF (polyvinylidene fluoride) TST R R ND ND R NR TST NR NR NR O-ring Materials MCE (mixed cellulose esters) NR R R NR ND R NR NR NR NR NR EPR (ethylene propylene rubber) NR NR NR R R R R NR NR R TST PES (polyether sulfone) GNR GR GNR GR ND GNR GNR ND NR NR ND Buna-N (nitrile rubber) NR NR NR TST R TST NR R R R NR NYL (nylon) RR TST R TST NR R ND R NR R Fluoroelastomer (vinylidiene O-ring Materials fluoride-hexafluoropropylene NR NR NR R R NR ND GR R R ND EPR (ethylene propylene rubber) R R NR R ND NR TST ND NR NR NR copolymer) Buna-N (nitrile rubber) NR R NR NR ND NR NR ND NR NR NR Silicone (silicone) NR TST NR TST R R GR R NR R ND Fluoroelastomer (vinylidiene Filter Holder Material fluoride-hexafluoropropylene NR RR TST TST R NR ND NR NR NR 316 SS (stainless steel) RRRRRRRRRRR copolymer) Silicone (silicone) NR R NR NR ND NR TST ND NR R R Filter Holder Material 316 SS (stainless steel) RRR LTD LTD R R R NR R R

www.merckmillipore.com 223 Chemical Compatibility of Filter Components

Chemical Compatibility of Merck Millipore Products This table shows the chemical compatibility of materials used in the fabrication of Merck Millipore devices and membranes. Please refer to the specification tables on product-specific pages for materials found in specific Merck Millipore devices. Guanidine Thiocyanate, Guanidine Thiocyanate, 5 M solution salt, aqueous Helium gas Hexane HC, aliphatic 1 N Acid, Hydrochloric (HCL) acid, inorganic 6 N Acid, Hydrochloric (HCL) acid, inorganic conc. Acid, Hydrochloric (HCL) acid, inorganic Acid Hydrofluoric acid, inorganic Hydrogen gas 3% Peroxide, Hydrogen peroxide 30% Peroxide, Hydrogen peroxide 90% Peroxide, Hydrogen peroxide Housing Materials HDPE (high density polyethylene) GR R LTD R R R R R R R NR PP (polypropylene) ND R NR GR TST NR NR R R TST R PS (polystyrene) ND ND NR R TST NR NR ND R R R PVC (polyvinyl chloride) ND ND NR GR TST NR NR R R TST R MMA (modified acrylic copolymer) GR ND GR GR ND ND GNR ND ND ND ND ABS (acrylonitrile-butadiene- ND ND GNR GR ND ND GNR ND ND ND ND styrene polymer) SAN (styrene-acrylonitrile ND ND GR ND ND ND ND ND ND ND ND polymer) PC (polycarbonate) ND R NR GR TST NR NR R R R R PET (polyethylene terephthalate) ND ND R GR R R NR R R R R Appendix EASTAR® (copolyester) ND ND R ND ND ND ND ND ND ND ND Filter Materials PP (polypropylene) ND R NR GR TST NR NR R R TST R PVC (polyvinyl chloride) ND ND NR GR TST NR NR R R TST R PC (polycarbonate) RRRRRR TST RRRR PTFE (polytetrafluoroethylene) GR R R R R R R R R R R PVDF (polyvinylidene fluoride) ND TST RR TST NR NR R R R R MCE (mixed cellulose esters) ND R GR GR NR GNR NR R NR NR NR PES (polyether sulfone) ND ND GR GR GR ND NR ND ND ND ND NYL (nylon) ND R R GR TST NR NR R R TST NR O-ring Materials EPR (ethylene propylene rubber) TST R NR NR NR NR NR R R R NR Buna-N (nitrile rubber) NR R R NR NR NR NR R NR NR NR Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene ND ND R GR TST NR NR R R R R copolymer) Silicone (silicone) ND R NR GR R R NR NR R R NR Filter Holder Material 316 SS (stainless steel) R R R R R NR NR R R R R

224 www.merckmillipore.com Chemical Compatibility of Filter Components

(aqueous (aqueous O Guanidine Thiocyanate, Guanidine Thiocyanate, 5 M solution salt, aqueous Helium gas Hexane HC, aliphatic 1 N Acid, Hydrochloric (HCL) acid, inorganic 6 N Acid, Hydrochloric (HCL) acid, inorganic conc. Acid, Hydrochloric (HCL) acid, inorganic Acid Hydrofluoric acid, inorganic Hydrogen gas 3% Peroxide, Hydrogen peroxide 30% Peroxide, Hydrogen peroxide 90% Peroxide, Hydrogen peroxide HYP solution) solution salt, aqueous Isobutyl Alcohol alcohol Acetate Isopropyl ester Alcohol Isopropyl alcohol Kerosene HC 50% Lactic Acid, acid, organic/alcohol PX Lubrol® solution) (aqueous surfactant/detergent Ketone Ethyl Methyl (MEK) ketone 0.1 M Mercaptoethanol, alcohol/mercaptan Acetate Methyl ester Alcohol Methyl alcohol Housing Materials Housing Materials HDPE (high density polyethylene) GR R LTD R R R R R R R NR HDPE (high density polyethylene) R R R R LTD TST R R ND TST R PP (polypropylene) ND R NR GR TST NR NR R R TST R PP (polypropylene) RR TST R TST R ND R ND TST R PS (polystyrene) ND ND NR R TST NR NR ND R R R PS (polystyrene) ND R NR R NR TST ND NR ND NR NR PVC (polyvinyl chloride) ND ND NR GR TST NR NR R R TST R PVC (polyvinyl chloride) R TST NR TST TST TST ND NR ND NR TST MMA (modified acrylic copolymer) GR ND GR GR ND ND GNR ND ND ND ND MMA (modified acrylic copolymer) GR TST GNR TST ND ND ND GNR ND GNR TST ABS (acrylonitrile-butadiene- ABS (acrylonitrile-butadiene- ND ND GNR GR ND ND GNR ND ND ND ND ND GR GNR GR ND ND ND TST ND GNR GR styrene polymer) styrene polymer)

SAN (styrene-acrylonitrile SAN (styrene-acrylonitrile Appendix ND ND GR ND ND ND ND ND ND ND ND ND GR ND GR ND ND ND ND ND ND GR polymer) polymer) PC (polycarbonate) ND R NR GR TST NR NR R R R R PC (polycarbonate) R R NR R R R TST NR ND NR TST PET (polyethylene terephthalate) ND ND R GR R R NR R R R R PET (polyethylene terephthalate) R ND R R TST R ND R ND TST ND EASTAR® (copolyester) ND ND R ND ND ND ND ND ND ND ND EASTAR® (copolyester) ND ND ND ND ND ND ND NR ND ND R Filter Materials Filter Materials PP (polypropylene) ND R NR GR TST NR NR R R TST R PP (polypropylene) RR TST R TST R ND R R TST R PVC (polyvinyl chloride) ND ND NR GR TST NR NR R R TST R PVC (polyvinyl chloride) R TST NR TST TST TST ND NR ND NR TST PC (polycarbonate) RRRRRR TST R ND NR R PC (polycarbonate) RRRRRR TST RRRR PTFE (polytetrafluoroethylene) GR RRR LTD GR TST R ND R R PTFE (polytetrafluoroethylene) GR R R R R R R R R R R PVDF (polyvinylidene fluoride) R R R R R TST ND NR ND NR R PVDF (polyvinylidene fluoride) ND TST RR TST NR NR R R R R MCE (mixed cellulose esters) R R NR NR R ND ND NR NR NR NR MCE (mixed cellulose esters) ND R GR GR NR GNR NR R NR NR NR PES (polyether sulfone) ND GR GNR GR GR ND ND GNR ND GNR GR PES (polyether sulfone) ND ND GR GR GR ND NR ND ND ND ND NYL (nylon) R TST R TST R TST ND R ND R TST NYL (nylon) ND R R GR TST NR NR R R TST NR O-ring Materials O-ring Materials EPR (ethylene propylene rubber) R R R R NR R ND R GR R R EPR (ethylene propylene rubber) TST R NR NR NR NR NR R R R NR Buna-N (nitrile rubber) R R NR R R R TST NR NR NR R Buna-N (nitrile rubber) NR R R NR NR NR NR R NR NR NR Fluoroelastomer (vinylidiene Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene R R NR R R R ND NR NR NR TST fluoride-hexafluoropropylene ND ND R GR TST NR NR R R R R copolymer) copolymer) Silicone (silicone) R R NR R NR R ND ND NR R Silicone (silicone) ND R NR GR R R NR NR R R NR Filter Holder Material Filter Holder Material 316 SS (stainless steel) RRRRRRRRRRR 316 SS (stainless steel) R R R R R NR NR R R R R

www.merckmillipore.com 225 Chemical Compatibility of Filter Components

Chemical Compatibility of Merck Millipore Products This table shows the chemical compatibility of materials used in the fabrication of Merck Millipore devices and membranes. Please refer to the specification tables on product-specific pages for materials found in specific Merck Millipore devices. zone zone Methylene Chloride Chloride Methylene HC, halogenated Isobutyl Ketone Methyl ketone Spirits Mineral HC 6 N Acid, Nitric acid, inorganic Acid Nitric (concentrated) acid, inorganic Nitrobenzene HC, aromatic Nitrogen gas (aqueous Nonidet-P40 solution) surfactant/detergent O gas Paraldehyde aldehyde Pentane HC, aliphatic Housing Materials HDPE (high density polyethylene) LTD R NR R NR R ND TST TST R LTD PP (polypropylene) NR NR NR TST NR NR ND ND NR TST NR PS (polystyrene) NR NR NR NR NR NR ND ND ND NR NR PVC (polyvinyl chloride) NR NR NR NR NR NR R ND NR TST NR MMA (modified acrylic copolymer) NR GNR ND ND ND NR ND ND ND ND GR ABS (acrylonitrile-butadiene- ND TST ND ND ND GNR ND ND ND ND GNR styrene polymer) SAN (styrene-acrylonitrile NR ND ND ND ND GNR ND ND ND ND GR polymer) PC (polycarbonate) NR NR NR TST NR NR ND TST R NR NR PET (polyethylene terephthalate) NR R ND R NR NR ND ND R ND R Appendix EASTAR® (copolyester) ND ND ND ND ND ND ND ND ND ND R Filter Materials PP (polypropylene) NR NR NR TST NR NR ND ND NR TST NR PVC (polyvinyl chloride) NR NR NR NR NR NR R ND NR TST NR PC (polycarbonate) NR NR R R R TST ND TST R NR R PTFE (polytetrafluoroethylene) R R R R ND R R ND GR GR GR PVDF (polyvinylidene fluoride) NR NR R R NR R R ND R TST GR MCE (mixed cellulose esters) NR GNR R NR GNR GNR R ND R NR GR PES (polyether sulfone) GNR GNR GR R ND ND ND ND ND ND GR NYL (nylon) TST R R NR NR R R ND NR R R O-ring Materials EPR (ethylene propylene rubber) NR R NR TST NR NR R TST TST GR NR Buna-N (nitrile rubber) NR NR R NR NR NR R TST NR R R Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene NR NR R R R R R ND NR NR GR copolymer) Silicone (silicone) NR NR NR NR NR NR R ND NR R NR Filter Holder Material 316 SS (stainless steel) R R R R NR NR R LTD NR R R

226 www.merckmillipore.com Chemical Compatibility of Filter Components

Chemical Compatibility of Merck Millipore Products This table shows the chemical compatibility of materials used in the fabrication of Merck Millipore devices and membranes. Please refer to the specification tables on product-specific pages for materials found in specific Merck Millipore devices. ils O Methylene Chloride Chloride Methylene HC, halogenated Isobutyl Ketone Methyl ketone Spirits Mineral HC 6 N Acid, Nitric acid, inorganic Acid Nitric (concentrated) acid, inorganic Nitrobenzene HC, aromatic Nitrogen gas (aqueous Nonidet-P40 solution) surfactant/detergent O zone gas Paraldehyde aldehyde Pentane HC, aliphatic Ether Petroleum ether Phenol (aqueous solution) phenol Hydroxide, Potassium 3 N caustic Pyridine amine Silicone silicone Sodium Carbonate solution) (aqueous solution salt, aqueous (Brine) Water solution salt, aqueous Sodium Chloride solution) (aqueous solution salt, aqueous Sulfate Sodium Dodecyl surfactant/detergent 3 N Sodium Hydroxide, caustic Sodium Hydroxide (concentrated) caustic Housing Materials Housing Materials HDPE (high density polyethylene) LTD R NR R NR R ND TST TST R LTD HDPE (high density polyethylene) LTD NR R R R TST RR TST RR PP (polypropylene) NR NR NR TST NR NR ND ND NR TST NR PP (polypropylene) ND NR R R R R R R ND R R PS (polystyrene) NR NR NR NR NR NR ND ND ND NR NR PS (polystyrene) ND NR R NR R ND ND ND ND R R PVC (polyvinyl chloride) NR NR NR NR NR NR R ND NR TST NR PVC (polyvinyl chloride) GNR NR R NR R R R R ND R NR MMA (modified acrylic copolymer) NR GNR ND ND ND NR ND ND ND ND GR MMA (modified acrylic copolymer) ND ND ND TST ND GR GR GR ND ND ND ABS (acrylonitrile-butadiene- ABS (acrylonitrile-butadiene- ND TST ND ND ND GNR ND ND ND ND GNR ND ND ND ND ND ND ND ND ND ND ND styrene polymer) styrene polymer)

SAN (styrene-acrylonitrile SAN (styrene-acrylonitrile Appendix NR ND ND ND ND GNR ND ND ND ND GR ND ND ND ND ND ND ND ND ND ND ND polymer) polymer) PC (polycarbonate) NR NR NR TST NR NR ND TST R NR NR PC (polycarbonate) R NR TST NR R R R R TST TST NR PET (polyethylene terephthalate) NR R ND R NR NR ND ND R ND R PET (polyethylene terephthalate) R GNR R NR ND TST R R ND TST NR EASTAR® (copolyester) ND ND ND ND ND ND ND ND ND ND R EASTAR® (copolyester) ND ND ND ND ND ND ND ND ND R ND Filter Materials Filter Materials PP (polypropylene) NR NR NR TST NR NR ND ND NR TST NR PP (polypropylene) NR NR R R R R R R ND R R PVC (polyvinyl chloride) NR NR NR NR NR NR R ND NR TST NR PVC (polyvinyl chloride) GNR NR R NR R R R R ND R NR PC (polycarbonate) NR NR R R R TST ND TST R NR R PC (polycarbonate) R NR NR NR R R R R TST NR NR PTFE (polytetrafluoroethylene) R R R R ND R R ND GR GR GR PTFE (polytetrafluoroethylene) GR GR R GR GR R R R ND R R PVDF (polyvinylidene fluoride) NR NR R R NR R R ND R TST GR PVDF (polyvinylidene fluoride) R R R NR R R R R ND R R MCE (mixed cellulose esters) NR GNR R NR GNR GNR R ND R NR GR MCE (mixed cellulose esters) R NR NR NR R R R R R NR NR PES (polyether sulfone) GNR GNR GR R ND ND ND ND ND ND GR PES (polyether sulfone) ND ND ND NR ND ND ND ND ND R R NYL (nylon) TST R R NR NR R R ND NR R R NYL (nylon) R NR R TST R TST R R ND R NR O-ring Materials O-ring Materials EPR (ethylene propylene rubber) NR R NR TST NR NR R TST TST GR NR EPR (ethylene propylene rubber) NR R R NR NR R R R TST RR Buna-N (nitrile rubber) NR NR R NR NR NR R TST NR R R Buna-N (nitrile rubber) R NR R NR R R R R GR R NR Fluoroelastomer (vinylidiene Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene NR NR R R R R R ND NR NR GR fluoride-hexafluoropropylene R R R NR R R R R R R R copolymer) copolymer) Silicone (silicone) NR NR NR NR NR NR R ND NR R NR Silicone (silicone) NR NR NR NR NR R R R R R R Filter Holder Material Filter Holder Material 316 SS (stainless steel) R R R R NR NR R LTD NR R R 316 SS (stainless steel) RRRRRR LTD R LTD R NR

www.merckmillipore.com 227 Chemical Compatibility of Filter Components

Chemical Compatibility of Merck Millipore Products This table shows the chemical compatibility of materials used in the fabrication of Merck Millipore devices and membranes. Please refer to the specification tables on product-specific pages for materials found in specific Merck Millipore devices. Sulfuric Acid Acid Sulfuric (concentrated) acid, inorganic (THF) Tetrahydrofuran ether Toluene HC, aromatic TCA solution) (aqueous acid, organic Trichloroethane HC, halogenated Trichloroethylene HC, halogenated 20 Tween® solution) (aqueous surfactant/detergent 8 M Urea, solution salt, aqueous Xylene HC, aromatic Housing Materials HDPE (high density polyethylene) R R LTD R LTD NR TST R LTD PP (polypropylene) NR NR NR R NR NR ND R NR PS (polystyrene) NR NR NR ND NR NR ND R NR PVC (polyvinyl chloride) NR NR NR NR NR NR ND TST NR MMA (modified acrylic copolymer) GNR NR NR ND GNR GNR ND GR NR ABS (acrylonitrile-butadiene- NR NR GNR ND ND ND ND ND GNR styrene polymer) SAN (styrene-acrylonitrile NR ND GNR ND ND ND ND ND GNR polymer) PC (polycarbonate) NR NR NR TST NR NR TST NR NR PET (polyethylene terephthalate) NR R ND NR TST R ND R NR Appendix EASTAR® (copolyester) ND ND NR ND ND ND ND ND ND Filter Materials PP (polypropylene) NR NR NR R NR NR ND R NR PVC (polyvinyl chloride) NR NR NR NR NR NR ND TST NR PC (polycarbonate) NR TST TST TST NR NR TST NR NR PTFE (polytetrafluoroethylene) R GR R GR R R R GR R PVDF (polyvinylidene fluoride) TST NR R R TST R TST RR MCE (mixed cellulose esters) NR NR GR NR NR NR R R GR PES (polyether sulfone) GNR ND R ND GNR GNR ND ND ND NYL (nylon) NR R R TST TST TST TST RR O-ring Materials EPR (ethylene propylene rubber) TST NR NR NR NR NR TST R NR Buna-N (nitrile rubber) NR NR NR NR NR NR R R NR Fluoroelastomer (vinylidiene fluoride-hexafluoropropylene R NR R NR R R R R R copolymer) Silicone (silicone) NR NR NR NR NR NR NR R NR Filter Holder Material 316 SS (stainless steel) NR NR R NR R R R R R

228 www.merckmillipore.com Chemical Compatibility of UF Products Microcon® Filter Microcon® Filter Centriprep® Plus-70 Centricon® Filter Filter Amicon® Ultra MC/CL with Ultrafree PVDF Membrane Ultrafiltration Disc Membrane) (Ultracel® Ultrafiltration Disc (Biomax® Membrane) Cells Stirred Solvents Acetone NR NR R ND NR R NR NR Acetonitrile NR NR R ND R R R R Acetonitrile (10%) RRRRRRRR Sulfuric Acid Acid Sulfuric (concentrated) acid, inorganic (THF) Tetrahydrofuran ether Toluene HC, aromatic TCA solution) (aqueous acid, organic Trichloroethane HC, halogenated Trichloroethylene HC, halogenated 20 Tween® solution) (aqueous surfactant/detergent 8 M Urea, solution salt, aqueous Xylene HC, aromatic Acetonitrile (40% in 1% TFA) TST TST R ND TST RRR Housing Materials Alconox® (1%) R R R ND R R R R HDPE (high density polyethylene) R R LTD R LTD NR TST R LTD Ammonium Acetate R R R ND R R R R PP (polypropylene) NR NR NR R NR NR ND R NR Ammonium Sulfate (50%) RRRRRRRR PS (polystyrene) NR NR NR ND NR NR ND R NR Ammonium Sulfate RRRRRRRR PVC (polyvinyl chloride) NR NR NR NR NR NR ND TST NR Amyl Alcohol NR NR R ND R R R R MMA (modified acrylic copolymer) GNR NR NR ND GNR GNR ND GR NR Benzyl Alcohol (1%) R R R ND R R R R ABS (acrylonitrile-butadiene- NR NR GNR ND ND ND ND ND GNR styrene polymer) n-Butanol NR NR TST ND TST TST TST TST

SAN (styrene-acrylonitrile CAPS (250 mM, pH 11) R R R ND TST RRR Appendix NR ND GNR ND ND ND ND ND GNR polymer) CHAPS (100 mM) R R R ND R R R R PC (polycarbonate) NR NR NR TST NR NR TST NR NR Decon-90 (1%) R R R ND R R R R PET (polyethylene terephthalate) NR R ND NR TST R ND R NR Diethyl Pyrocarbonate (DEPC, 0.2%) RRRRRRRR EASTAR® (copolyester) ND ND NR ND ND ND ND ND ND Digitonin (0.5%) in EtOH R R R ND R R R R Filter Materials Dimethylacetamide NR NR TST ND NR TST NR NR PP (polypropylene) NR NR NR R NR NR ND R NR Dimethylformamide NR NR TST ND NR TST NR NR PVC (polyvinyl chloride) NR NR NR NR NR NR ND TST NR Dimethylformamide (10%) RRRRRRRR PC (polycarbonate) NR TST TST TST NR NR TST NR NR Dioxane NR NR TST ND TST TST TST TST PTFE (polytetrafluoroethylene) R GR R GR R R R GR R Dioxane (10%) R R R ND R R R R PVDF (polyvinylidene fluoride) TST NR R R TST R TST RR Dithiothreitol (0.1M) TST RRRRRRR MCE (mixed cellulose esters) NR NR GR NR NR NR R R GR DMSO (10%) R R R ND R R R R PES (polyether sulfone) GNR ND R ND GNR GNR ND ND ND DMSO NR NR TST ND NR TST NR NR NYL (nylon) NR R R TST TST TST TST RR DTT/Benzamidine HCI (1 mM each) R R R ND R R R R O-ring Materials Ethanol TST NR TST ND TST TST TST TST EPR (ethylene propylene rubber) TST NR NR NR NR NR TST R NR Ethanol (10%) RRRRRRRR Buna-N (nitrile rubber) NR NR NR NR NR NR R R NR Ethylene Glycol NR NR TST ND TST TST TST TST Fluoroelastomer (vinylidiene Ethylene Glycol (10%) R R R ND R R R R fluoride-hexafluoropropylene R NR R NR R R R R R Formaldehyde (5%) RRRRRRRR copolymer) Formamide NR NR TST ND NR TST NR NR Silicone (silicone) NR NR NR NR NR NR NR R NR Glycerine (Glycerol) RRRRRRRR Filter Holder Material Guanidine HCI (6 M) RRRRRRRR 316 SS (stainless steel) NR NR R NR R R R R R Guanidine Thiocyanate (0.5 M) R R R ND R R R R Guanidine Thiocyanate (5 M) NR NR R ND NR R R R Hydrogen Peroxide (3%) R R R ND R R R R Hydrogen Peroxide/MeOH (1:3) NR NR TST ND TST TST TST TST

www.merckmillipore.com 229 Chemical Compatibility of UF Products Microcon® Filter Microcon® Filter Centriprep® Plus-70 Centricon® Filter Filter Amicon® Ultra MC/CL with Ultrafree PVDF Membrane Ultrafiltration Disc Membrane) (Ultracel® Ultrafiltration Disc (Biomax® Membrane) Cells Stirred Solvents Hydroxylamine (2 M) R R R ND R R R R Imidazole (100 mM) R R R ND R R R R Imidazole (300 mM) R R R ND R R R R Isobutyl Alcohol NR NR TST ND NR TST TST TST Isopropyl Alcohol NR NR TST ND NR TST TST TST Isopropyl Alcohol (10%) R R R ND R R R R Lubrol® PX (0.1%) R R R ND R R R R Mercaptoethanol (0.1 M) RRRRRRRR Methanol NR NR TST ND NR TST TST TST Methanol (10%) RRRRRRRR Nonidet P-40 (2%) RRRR TST RRR Paraldehyde TST TST R ND R R R R Phenol (5%) R R R ND R R R R Phosphate Buffer (1 M, pH 8.2) RRRRRRRR Polyethylene Glycol (10%) RRRRRRRR Appendix Propanol NR NR TST ND NR TST NR NR Propanol (10%) RRRRRRRR Sodium Carbonate (20%) RRRRRRRR Sodium Cholate R R R ND R R R R Sodium Chloride (2 M) R R R ND R R R R Sodium Deoxycholate (5%) RRRRRRRR Sodium Dodecyl Sulfate (3%) R R R ND R R R R Sodium Dodecyl Sulfate (2%) R R R ND R R R R Sodium Dodecyl Sulfate (0.1 M) R R R ND R R R R Sodium Thiocyanate (3 M) TST TST R ND R R R R Terg-A-Zyme (1%) RRRRRRRR Tetrahydrofuran NR NR TST ND NR TST NR NR Triethylamine (2%) R R R ND R R R R Tris Buffer (1 M, pH 8.2) RRRRRRRR Triton® X-100 (5 mM) RRRRRRRR Tween® 20 (0.1%) RRRRRRRR Urea (8 M) RRRRRRRR

230 www.merckmillipore.com Chemical Compatibility of UF Products Microcon® Filter Microcon® Filter Centriprep® Plus-70 Centricon® Filter Filter Amicon® Ultra MC/CL with Ultrafree PVDF Membrane Ultrafiltration Disc Membrane) (Ultracel® Ultrafiltration Disc (Biomax® Membrane) Cells Stirred Acids Acetic Acid, 10% RRRRRRRR Acetic Acid (glacial) NR NR NR ND R NR TST TST Boric Acid R R R ND R R R R Formic Acid (5%) RRRRRRRR Formic Acid (70%) NR NR NR ND R NR R R Hydrochloric Acid (1.0 N) R R R R R NR R R Lactic Acid (50%) RRRRRRRR Nitric Acid (10%) RRR TST RRRR Perchloric Acid (5%) TST TST R ND TST RRR Phosphoric Acid (5%) RRRRRRRR Sulfamic Acid (3%) R R R ND R R R R Appendix Sulfuric Acid (3%) RRRRRRRR Trichloroacetic Acid (10%) RRRRRRRR Trichloroacetic Acid (50%) NR NR NR ND NR R NR TST Trifluoroacetic Acid (10%) RRRRRRRR Trifluoroacetic Acid (50%) NR R NR TST NR R NR TST Bases Ammonium Hydroxide (5%) RRRRRRRR Ammonium Hydroxide (6 N) R R R ND R R R R Sodium Hydroxide (0.1 N) R R R ND R R R R Sodium Hydroxide (2.5 N) NR R R ND R NR R NR

The following descriptions are abbreviated. Please see the beginning of this section for complete information. R = Recommended; NR = Not Recommended; TST = Testing Recommended; ND = No Data Presently Available www.merckmillipore.com 231 Chemical Compatibility Guide for MultiScreen® Filter Plates Ratings are based on 100% or concentrated solutions, unless otherwise indicated. Aromatic hydrocarbons (especially ketones, DMF, DMAC, DMSO, THF, acetonitrile) and chlorinated hydrocarbons will attack both membranes and plastics. A chemical listed as NR may be suitable in low concentrations for short exposures, but this must be determined on an individual assay basis.

Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents

Barex®/TiO2/ Acrylic or Barex®/

Plate Material/Membrane Polyolefin Acrylic or Classic Acrylic or Styrene/ Durapore®, MCE, TiO2/Styrene, copolymer/PTFE Styrene/Durapore® MCE DEAE Immobilon®-P Acids Acetic (5%) R R R R R Acetic, Glacial R L to NR NR R/L to NR L Boric R R R R R Trichloroacetic (<20%) R R R R R Trichloroacetic (20 to 40%) R R L R/L to NR L Trichloroacetic (>40%) R L to NR NR L/NR NR Hydrochloric (0.1 N) R R R R R Hydrochloric (1 N) R R L to NR R/L to NR R to L Hydrochloric (>1 N) R R NR R/L to NR R Hydrofluoric R R (20% max.) NR NR R (20% max.) Nitric (conc) R L to NR NR NR L Sulfuric (conc) R L NR L/NR L Bases Appendix

NH4OH (6 N) R L to NR NR L to NR R NaOH (0.1 N) R R to L L to NR R/L to NR R NaOH (1 N) R L to NR NR L/NR L NaOH (>6 N) R NR NR NR NR Urea R R — R (Durapore® only) R Triethylamine R R R R R Diethanolamine (≤3 mol) R R R R R Alcohols Amyl R R NR R/NR R Benzyl (1%) R R R R R Butyl R R R R R Ethanol (40%) R R NR R R R (no filtrate Ethanol (≥50%) R R NR R/NR collection) Isobutyl R R R R R Isopropyl R R L to NR R/L to NR R Methanol R R to L NR R/NR R Triton® X-100 Surfactant (<1%) R R R R R

R = Recommended, no known restrictions, NR = Not recommended, membrane or plate severely attacked by chemical, L = Limited, chemical resistance marginal, short time exposures should be tested individually for application. Dilution with water or other non-solvent will likely enhance compatibility

1. Scintillants should only be used with Barex® or SAN plates.

2. Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

232 www.merckmillipore.com Chemical Compatibility Guide for MultiScreen® Filter Plates

Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents

Barex®/TiO2/ Acrylic or Barex®/

Polyolefin Acrylic or Classic Acrylic or Styrene/ Durapore®, MCE, TiO2/Styrene, Plate Material/Membrane copolymer/PTFE Styrene/Durapore® MCE DEAE Immobilon®-P

Barex®/TiO2/ Acrylic or Barex®/ Solvents Plate Material/Membrane Polyolefin Acrylic or Classic Acrylic or Styrene/ Durapore®, MCE, TiO /Styrene, 2 Acetone R NR NR NR NR copolymer/PTFE Styrene/Durapore® MCE DEAE Immobilon®-P Acetonitrile (no filtrate collection, Acids centrifuge) R NR NR NR L (<35%) Acetic (5%) R R R R R Amyl Acetate R NR NR R/NR R Acetic, Glacial R L to NR NR R/L to NR L Carbon Tetrachloride R L to NR L to NR R R Boric R R R R R Chloroform R NR NR NR NR Trichloroacetic (<20%) R R R R R Cyclohexanone R NR to L NR to L R/ NR to L NR to L Trichloroacetic (20 to 40%) R R L R/L to NR L DMAC R NR NR NR NR Trichloroacetic (>40%) R L to NR NR L/NR NR DMF R NR NR NR NR Hydrochloric (0.1 N) R R R R R DMSO (no filtrate collection) R L (70%) NR NR L (10%) max. Hydrochloric (1 N) R R L to NR R/L to NR R to L Ethylene Glycol R R R R R Hydrochloric (>1 N) R R NR R/L to NR R Formaldehyde R R (10% max.) NR R (<40%) /NR R (<40%) Appendix Hydrofluoric R R (20% max.) NR NR R (20% max.) Hexane NR R R R R Nitric (conc) R L to NR NR NR L Methylene Chloride L NR NR NR NR Sulfuric (conc) R L NR L/NR L MEK R NR NR NR NR Bases MIBK R NR NR L/NR NR/L NH4OH (6 N) R L to NR NR L to NR R Phenol (5%) R L to NR L to NR R R NaOH (0.1 N) R R to L L to NR R/L to NR R Pyridine R R R R R NaOH (1 N) R L to NR NR L/NR L Scintillants NR NR NR R NR/R(1) NaOH (>6 N) R NR NR NR NR Triethylamine R R R R R Urea R R — R (Durapore® only) R Toluene NR NR to L NR to L R NR to L/R Triethylamine R R R R R THF R NR NR L/NR NR/L Diethanolamine (≤3 mol) R R R R R Xylene R NR NR R R Alcohols Other Organics Amyl R R NR R/NR R Attophos® Reagent R R R R R Benzyl (1%) R R R R R Fluorescein R R R R R Butyl R R R R R Glycerine R R R R R Ethanol (40%) R R NR R R Hydrogen Peroxide R R NR R (3%) R R (no filtrate Ethanol (≥50%) R R NR R/NR Polyethylene Glycol R R R R R collection) Tween® Surfactant (<5%)(2) R R R R R Isobutyl R R R R R Triton® X-100 Surfactant (<1%) R R R R R Isopropyl R R L to NR R/L to NR R Triton® X-100 Surfactant (<5%) R R L R/L R Methanol R R to L NR R/NR R Inorganic Salts Triton® X-100 Surfactant (<1%) R R R R R Carbon Disulfite R NR NR NR NR Sodium Hypochlorite R R NR R (5%)/ NR R

1. Scintillants should only be used with Barex® or SAN plates.

2. Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant-containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

www.merckmillipore.com 233 Chemical Compatibility Guide for the MultiScreen® Vacuum Manifold

The following tables outline the solvents that have been evaluated for compatibility with the various components of the MultiScreen®HTS vacuum manifold. In general the manifold will tolerate the same chemicals as the rest of the system.

However, when using concentrated acids, it is important to rinse through the entire manifold at the end of procedures, especially if collection is done through the manifold into a trap, rather than using the collection trays.

MultiScreen®HTS Vacuum Manifold Components

Manifold Base/ Collar Gasket Droplet Trap Component Frame Gaskets/Tubing Standard Collar Support Grid Tubing Fittings Array HDPE/ PP with EPDM or Polypropylene, Fluoroelastomer Materials of Construction Polyolefin Silicone Nylon Stainless Steel Seals PTFE Acetone R G G E G E Acetonitrile E G E E G E Dimethyl Formamide (DMF) E G R E G E Dimethyl Sulfoxide (DMSO) E G E E G E Ethyl Acetate E G E E G E Ethanol E G G E E E Formic Acid E G NR G G R Appendix Hexane NR NR R E G E Hydrochloric Acid (37%) E R NR R R R Isopropanol E E R E E E Methanol E E R E E E Methylene Chloride NR NR R E R E Sodium Hypochlorite E G NR G G R Tetrahydrofuran (THF) R NR E E NR E Toluene NR NR E E R E Trichloroacetic Acid (TCA) E NR G G R R Trifluoroacetic Acid (TFA) E NR R R G R

E = Excellent performance, G = Good performance, R = Rinse after contact, NR = Not recommended

234 www.merckmillipore.com Index by Catalogue Number

Catalogue No. Page Catalogue No. Page Catalogue No. Page 000205...... 196 1.52058.0001...... 208 5123...... 105–106 009507P...... 193–194 1.53172.0001...... 208 5124...... 105–106 08-110...... 28–29 122640...... 75–76 5125...... 105–106 08-115...... 28–29 122641...... 75–76 5162...... 140 08-126...... 28–29 122642...... 75–76 5163...... 140 1.02014.0001...... 199 122643...... 75–76 524624...... 76–77 1.02016.0001...... 199 13899...... 144 524625...... 76–77 1.02021.0001...... 199 16-125...... 83 524627...... 76–77 1.02129.0001...... 208 16-126...... 86–87 524628...... 76–77 1.05586.0001...... 211 16-156...... 83 524650...... 77 1.05642.0001...... 211 16-266...... 83 539131...... 76–77 1.05715.0001...... 211 17-10195...... 75–76 539132...... 76–77 1.15093.0001...... 200 17-10210...... 75–76 539133...... 76–77 1.15094.0001...... 200 2005-25GM...... 63 539134...... 76–77 1.15095.0001...... 200 2010-100GM...... 63 539136...... 76–77 1.15096.0001...... 200 2020-500GM...... 63 539137...... 76–77 1.15389.0001...... 211 2060...... 122 539138...... 76–77 1.16225.0001...... 211 2070-100GM...... 63 539180...... 75–76 1.19127.0001...... 199 2081-100GM...... 63 539720...... 75–76 1.19698.0001...... 199 2082-250GM...... 63 539722...... 75–76 Appendix 1.19767.0001...... 199 2090-100GM...... 63 539779...... 75–76 1.19847.0001...... 199 2120-100GM...... 63 539790...... 75–76 1.19870.0001...... 199 2125-500GM...... 63 5614...... 193–194 1.19941.0001...... 199 2500...... 122 565000...... 76–77 1.50144.0001...... 209 2502...... 122 6003...... 105–106

1.50169.0001...... 209 2504...... 122 6015...... 105–106 Index 1.50247.0001...... 209 220201...... 77 6028...... 105–106 1.50356.0001...... 209 300410...... 77 625718...... 77 1.50398.0001...... 209 391338...... 77 650212...... 75–76 1.50447.0001...... 211 4.86505.0500 ...... 62 6504-20L...... 62 1.50453.0001...... 211 4.86505.1000 ...... 62 6505-4L...... 62 1.50462.0001...... 211 4.86505.5000 ...... 62 69022-3...... 90–91 1.50464.0001...... 211 4.86505.9010 ...... 62 69025-3...... 86–87 1.50470.0001...... 209 4.86505.9020 ...... 62 69026-3...... 86–87 1.50601.0001...... 210 4.86505.9200 ...... 62 69036-3...... 90–91 1.50604.0001...... 210 4104...... 104 69037-3...... 90–91 1.50635.0001...... 210 4302...... 102 69038-3...... 90–91 1.50638.0001...... 210 4303...... 102 69051-3...... 90–91 1.50641.0001...... 210 4304...... 102 69065-3...... 86–87 1.50648.0001...... 209 4305...... 102 69066-3...... 90–91 1.50657.0001...... 211 4306...... 102 69067-3...... 90–91 1.50660.0001...... 211 4307...... 102 69068-3...... 90–91 1.50830.0001...... 210 4310...... 102 69068-4...... 90–91 1.50834.0001...... 210 4311...... 102 69069-3...... 90–91 1.50836.0001...... 210 444810...... 75–76 69203-3...... 86–87 1.50892.0001...... 210 4610-100ML...... 62 69232-3...... 86–87 1.51453.0001...... 209 4650-500ML...... 62 69284-3...... 53–54 1.51465.0001...... 208 4670-4L...... 62 69284-4...... 53–54 1.52001.0001...... 208 5.04865.0001...... 60 693017...... 77 1.52006.0001...... 208 5.04866.0001...... 60 69453-3...... 53–54 1.52022.0001...... 208 5.04867.0001...... 60 69453-4...... 53–54 1.52028.0001...... 208 5121...... 105–106 69670-3...... 86–87 1.52048.0001...... 208 5122...... 105–106 69670-4...... 86–87 www.merckmillipore.com 235 Catalogue No. Page Catalogue No. Page Catalogue No. Page 69670-5...... 86–87 71003-4...... 50 71401-3...... 53–54 69671-3...... 90–91 71003-5...... 50 71401-4...... 53–54 69672-3...... 90–91 71009-3...... 72 71408-3...... 53–54 69704-3...... 86–87 71009-4...... 72 71408-4...... 53–54 69704-4...... 86–87 71011-3...... 53–54 71412-3...... 74 69755-3...... 86–87 71011-4...... 53–54 71456-3...... 71–72 6976...... 204 71085-3...... 50 71456-4...... 71–72 6977...... 204 71086-3...... 50 71491-3...... 59 6978...... 204 71086-4...... 50 71491-4...... 59 6979...... 204 71086-5...... 50 71491-5...... 59 70181-3...... 53–54 71087-3...... 50 71493-3...... 90–91 70181-4...... 53–54 71087-4...... 50 71504-3...... 95 70235-3...... 53–54 71091-3...... 50 71505-3...... 95 70235-4...... 53–54 71091-4...... 50 71506-3...... 95 70236-3...... 53–54 71092-3...... 71–72 71507-3...... 95 70236-4...... 53–54 71092-4...... 71–72 71508-3...... 95 70239-3...... 86–87 71092-5...... 71–72 71509-3...... 95 70534-3...... 86–87 71110-3...... 74 71510-3...... 95 70541-3...... 86–87 71110-4...... 74 71511-3...... 95 70541-4...... 86–87 71110-5...... 74 71512-3...... 95 70541-5...... 86–87 71115-3...... 58 71513-3...... 95 70584-3...... 71–72 71115-4...... 58 71514-3...... 95 70584-4...... 71–72 71183-3...... 72 71532-3...... 75–76 70664-3...... 74 71186-3...... 73 71537-3...... 90–91 Appendix 70666-3...... 86–87 71186-4...... 73 71540-3...... 90–91 70666-4...... 86–87 71187-3...... 72 71592-3...... 86–87 70666-5...... 86–87 71187-4...... 72 71592-4...... 86–87 70691-3...... 86–87 71194-3...... 73 71608-3...... 86–87 Index 70691-4...... 86–87 71194-4...... 73 71610-3...... 86–87 70691-5...... 86–87 71205-3...... 74 71613-3...... 86–87 70746-3...... 74 71206-3...... 74 71712-3...... 95 70746-4...... 74 71227-3...... 53–54 71713-3...... 95 70750-3...... 71–72 71227-4...... 53–54 71739-3...... 95 70751-3...... 71–72, 86–87 71230-3...... 74 71739-4...... 95 70793-3...... 71–72, 86–87 71230-4...... 74 71740-3...... 95 70794-3...... 71–72, 86–87 71230-5...... 74 71740-4...... 95 70899-3...... 86–87 71259-3...... 58 71742-3...... 95 70909-3...... 72 71259-4...... 58 71742-4...... 95 70909-4...... 72 71259-5...... 58 71743-3...... 95 70921-3...... 71–72 71281-3...... 58 71743-4...... 95 70921-4...... 71–72 71281-4...... 58 71745-3...... 95 70921-5...... 71–72 71296...... 76–77 71745-4...... 95 70922-3...... 71–72 71296-3...... 72 71746-3...... 95 70922-4...... 71–72 71296-4...... 72 71746-4...... 95 70922-5...... 71–72 71300-3...... 59 71748-3...... 95 70923-3...... 71–72 71300-4...... 59 71757-3...... 59 70923-4...... 71–72 71366-3...... 59 71757-4...... 59 70967-3...... 58 71366-4...... 59 71757-5...... 59 70967-4...... 58 71370-3...... 71–72 71759-3...... 59 70967-5...... 58 71370-4...... 71–72 71759-4...... 59 70967-6...... 58 71400-3...... 53–54 71760-3...... 59 71003-3...... 50 71400-4...... 53–54 71771-3...... 75–76

236 www.merckmillipore.com Catalogue No. Page Catalogue No. Page Catalogue No. Page 71772...... 75–76 ABN2...... 43–44 AP1504200...... 187–188 71777-3...... 75–76 ACK5003GS...... 80–81 AP1504700...... 187–188 71789-3...... 59 ACK5003NT...... 80–81 AP1507500...... 187–188 71842-3...... 50 ACK5003PA...... 80–81 AP1509000...... 187–188 71842-4...... 50 ACK5003PG...... 80–81 AP1512450...... 187–188 71902-3...... 58 ACK5010GS...... 80–81 AP1514250...... 187–188 71902-4...... 58 ACK5010NT...... 80–81 AP2001300...... 187–188 71975-3...... 50 ACK5010PA...... 80–81 AP2002500...... 187–188 72103...... 75–76 ACK5010PG...... 80–81 AP2004200...... 187–188 72181-3...... 58 ACK5030GS...... 80–81 AP2004700...... 187–188 72181-4...... 58 ACK5030NT...... 80–81 AP2005500...... 187–188 72181-5...... 58 ACK5030PA...... 80–81 AP2007500...... 187–188 72622-3...... 58 ACK5030PG...... 80–81 AP2009000...... 187–188 72622-4...... 58 ACK5050GS...... 80–81 AP2012450...... 187–188 72635-1KIT...... 56 ACK5050NT...... 80–81 AP2014250...... 187–188 72636-1KIT...... 56 ACK5050PA...... 80–81 AP2501000...... 187–188 7910-500GM...... 62 ACK5050PG...... 80–81 AP2501300...... 187–188 7960-5KG...... 62 ACK5100GS...... 80–81 AP2502200...... 187–188 8000SCPKIT...... 105–106 ACK5100NT...... 80–81 AP2502500...... 187–188 8003SCPKIT...... 105–106 ACK5100PA...... 80–81 AP2504200...... 187–188 Appendix 8010SCPKIT...... 105–106 ACK5100PG...... 80–81 AP2504700...... 187–188 8050SCPKIT...... 105–106 ACR5000GS...... 80–81 AP2509000...... 187–188 8200SCPKIT...... 105–106 ACR5000NT...... 80–81 AP2512450...... 187–188 8400SCPKIT...... 105–106 ACR5000PA...... 80–81 AP2514250...... 187–188 9031...... 104 ACR5000PG...... 80–81 AP30034P0...... 139

9051...... 104 ACS500024...... 80–81 AP3212450...... 139 Index 9601-100ML ...... 62 ACS500312...... 80–81 AP4001000...... 187–188 9602-500ML ...... 62 ACS500324...... 80–81 AP4002405...... 187–188 9610-1L ...... 62 ACS501012...... 80–81 AP4002500...... 187–188 9612-5L ...... 62 ACS501024...... 80–81 AP4003705...... 187–188 9613-10L ...... 62 ACS503012...... 80–81 AP4007000...... 187–188 A-003-E...... 28–29 ACS503024...... 80–81 AP4009000...... 187–188 AABG01300...... 172–174 ACS505012...... 80–81 AP4014250...... 187–188 AABG02500...... 172–174 ACS505024...... 80–81 APFA04700...... 187–188 AABG03700...... 172–174 ACS510012...... 80–81 APFB02500...... 187–188 AABG04700...... 172–174 ACS510024...... 80–81 APFB03700...... 187–188 AABP02500...... 172–174 AG4502550...... 135 APFB04700...... 187–188 AABP04700...... 172–174 AG56P...... 28–29 APFC02500...... 187–188 AAWG01300...... 172–174 AN0604700...... 188–189 APFC04700...... 187–188 AAWG0250C...... 172–174 AN1204700...... 188–189 APFC09050...... 187–188 AAWG03700...... 172–174 AN1H04700...... 188–189 APFD02500...... 187–188 AAWG04700...... 172–174 AN2504700...... 188–189 APFD04700...... 187–188 AAWP01300...... 172–174 AN3H04700...... 188–189 APFD09050...... 187–188 AAWP02500...... 172–174 AN5004700...... 188–189 APFF02500...... 187–188 AAWP03700...... 172–174 ANWP02500...... 184–185 APFF04700...... 187–188 AAWP037P0...... 172–174 ANWP04700...... 184–185 APFF09050...... 187–188 AAWP037PM...... 172–174 AP1001300...... 139 APFF14250...... 187–188 AAWP04700...... 172–174 AP1002500...... 139 AQFA03700...... 187–188 AAWP0470M...... 172–174, 213 AP1003700...... 139 AQFA04700...... 187–188 AAWP09000...... 172–174 AP1004700...... 139 AQFA09050...... 187–188 AAWP14250...... 172–174 AP1502500...... 187–188 AQFA8X105...... 187–188 ABI1...... 43–44 AP1503700...... 187–188 ASTM03701...... 212 www.merckmillipore.com 237 Catalogue No. Page Catalogue No. Page Catalogue No. Page ATTP01300...... 182–183 ELI-004-H...... 35–36 FHLP14250...... 178–179 ATTP02500...... 182–183 ELI-004-M...... 35–36 FHUP04700...... 178–179, 197 ATTP03700...... 182–183 ELI-006-H...... 35–36 FSLW02500...... 178–179 ATTP04700...... 182–183 ELI-006-M...... 35–36 FSLW03700...... 178–179 ATTP14250...... 182–183 ELI-008-H...... 35–36 FSLW04700...... 178–179 B04A-03-5PK...... 43–44 ELI-008-M...... 35–36 FSLW09025...... 178–179 C04A-01-5PK...... 43–44 ELI-010-H...... 35–36 FSLW14200...... 178–179 CC050...... 28–29 ELI-012-H...... 35–36 GM230...... 43–44 CC052...... 28–29 ELI-014-M...... 35–36 GNWP02500...... 184–185 CC054...... 28–29 ELI-016-H...... 35–36 GNWP04700...... 184–185 CC065...... 28–29 ELI-016-M...... 35–36 GPWP01300...... 177 CC076...... 28–29 ELI-018-H...... 35–36 GPWP02500...... 177 CC077...... 28–29 ELI-018-M...... 35–36 GPWP04700...... 177 CC078...... 28–29 EV262...... 43–44 GPWP09050...... 177 CC080...... 28–29 EZFITBACKF...... 206 GPWP14250...... 177 CC083...... 28–29 EZFITFRIT3...... 206 GSTF01300...... 172–174 CC085...... 28–29 EZFITGASK3...... 206 GSTF02500...... 172–174 CC086...... 28–29 EZFITL0W01...... 206 GSTF04700...... 172–174 CC095...... 28–29 EZFITL0W03...... 206 GSTF09000...... 172–174 CC115...... 28–29 EZFITL0W06...... 206 GSTF14250...... 172–174 CC117...... 28–29 EZFITMAKIT...... 206 GSWP01300...... 172–174 CC118...... 28–29 EZFITMIC03...... 132 GSWP02500...... 172–174 CC145...... 28–29 EZFITQUICKC...... 206 GSWP03700...... 172–174 DAWP01300...... 172–174 EZFITVALV1...... 206 GSWP04700...... 172–174 Appendix DAWP02500...... 172–174 EZSTREAM1...... 206 GSWP09000...... 172–174 DAWP04700...... 172–174 F1903...... 28–29 GSWP14250...... 172–174 DAWP09025...... 172–174 F1904...... 28–29 GTBP01300...... 182–183 DAWP14250...... 172–174 F84-GL3-GK...... 43–44 GTBP02500...... 182–183 Index DDAC00010-4P...... 115 F84-HG3-GK...... 43–44 GTBP04700...... 182–183 DDAC00010-8P...... 115 FALP01300...... 178–179 GTTP01300...... 182–183 DDAC00010-GR...... 115 FALP02500...... 178–179 GTTP02500...... 182–183 DDHW00010-WW...... 115 FALP03700...... 178–179 GTTP03700...... 182–183 DDSP00010-DE...... 115 FALP04700...... 178–179 GTTP04700...... 182–183 DTTP01300...... 182–183 FALP09050...... 178–179 GTTP09030...... 182–183 DTTP02500...... 182–183 FALP14250...... 178–179 GTTP14250...... 182–183 DTTP04700...... 182–183 FC010...... 28–29 GVHP00010...... 175–176 DVPP01300...... 175–176 FC010-100MG...... 28–29 GVHP01300...... 175–176 DVPP02500...... 175–176 FC010-10MG...... 28–29 GVHP02500...... 175–176 DVPP04700...... 175–176 FC010-5MG...... 28–29 GVHP04700...... 175–176 DVPP08250...... 175–176 FC014...... 28–29 GVHP09050...... 175–176 DVPP09050...... 175–176 FGLP01300...... 178–179 GVHP14250...... 175–176 DVPP14250...... 175–176 FGLP02500...... 178–179 GVWP01300...... 175–176 DVPP29325...... 175–176 FGLP04700...... 178–179 GVWP02500...... 175–176 ECM101...... 28–29 FGLP09050...... 178–179 GVWP04700...... 175–176 ECM102...... 28–29 FGLP14250...... 178–179 GVWP09050...... 175–176 ECM103...... 28–29 FHLC01300...... 181 GVWP10050...... 175–176 ECM104...... 28–29 FHLC02500...... 181 GVWP14250...... 175–176 ECM105...... 28–29 FHLC04700...... 181 GWSC04501...... 143 ECM205...... 28–29 FHLP01300...... 178–179 GWSC04510...... 143 ECM541...... 28–29 FHLP02500...... 178–179 GWSC04550...... 143 ECM542...... 28–29 FHLP03700...... 178–179 GWSC10001...... 143 ECM546...... 28–29 FHLP04700...... 178–179 GWSC10010...... 143 ELI-002-M...... 35–36 FHLP09050...... 178–179 GWSC50001...... 143

238 www.merckmillipore.com Catalogue No. Page Catalogue No. Page Catalogue No. Page H20CC0106...... 10 HVLP09050...... 175–176 JMWP04700...... 178–179 H20CC0124...... 10 IBFP0785C...... 116 JMWP09025...... 178–179 H20CC0501...... 10 IBFP0813C...... 116 JMWP14225...... 178–179 H20CC0506...... 10 IP02-1.5ML...... 83 JVWP01300...... 178–179 H20CC1001...... 10 IP04-1.5ML...... 83 JVWP02500...... 178–179 H20CC1006...... 10 IP05-1.5ML...... 83 JVWP04700...... 178–179 HABG01300...... 172–174 IP10-10ML...... 83 JVWP09025...... 178–179 HABG02500...... 172–174 IPFL00010...... 116 JVWP14225...... 178–179 HABG04700...... 172–174, 213 IPFL07810...... 116 LCWG02500...... 180 HABP02500...... 172–174 IPFL10100...... 116 LCWG04700...... 180 HABP04700...... 172–174 IPFL20200...... 116 LCWP01300...... 180 HATF01300...... 172–174 IPSN07852...... 116 LCWP02500...... 180 HATF02500...... 172–174 IPSN08132...... 116 LCWP04700...... 180 HATF04700...... 172–174 IPVH00010...... 116 LCWP09025...... 180 HATF09025...... 172–174 IPVH07850...... 116 LCWP14250...... 180 HATF14250...... 172–174 IPVH08100...... 116 LSK2ABA20...... 83 HAWG01300...... 172–174 IPVH08130...... 116 LSK2ABG20...... 83 HAWG02500...... 172–174 IPVH09120...... 116 LSKAST100...... 55 HAWG03700...... 172–174 IPVH10100...... 116 LSKCLS500...... 55 HAWG04700...... 172–174, 213 IPVH15150...... 116 LSKCRS500...... 55 Appendix HAWP01300...... 172–174 IPVH20200...... 116 LSKCTB500...... 55 HAWP02400...... 172–174 IPVH304F0...... 116 LSKMAG03CBX02...... 88–89 HAWP02500...... 172–174 ISEQ00010...... 116 LSKMAG03CBX10...... 88–89 HAWP03700...... 172–174 ISEQ07850...... 116 LSKMAG1CBX02...... 88–89 HAWP04700...... 172–174, 213 ISEQ08100...... 116 LSKMAG1CBX10...... 88–89

HAWP0470M...... 172–174, 213 ISEQ08130...... 116 LSKMAG25CBX02...... 88–89 Index HAWP05000...... 172–174 ISEQ09120...... 116 LSKMAG25CBX10...... 88–89 HAWP09000...... 172–174 ISEQ10100...... 116 LSKMAGA02...... 88–89 HAWP14250...... 172–174 ISEQ15150...... 116 LSKMAGA10...... 88–89 HNWP02500...... 184–185 ISEQ20200...... 116 LSKMAGAG02...... 88–89 HNWP04700...... 184–185 ISEQ26260...... 116 LSKMAGAG10...... 88–89 HPWP01300...... 177 JAWP01300...... 178–179 LSKMAGD12...... 88–89 HPWP02500...... 177 JAWP02500...... 178–179 LSKMAGG02...... 88–89 HPWP04700...... 177 JAWP04700...... 178–179 LSKMAGG10...... 88–89 HPWP09050...... 177 JAWP09025...... 178–179 LSKMAGH02...... 88–89 HPWP14250...... 177 JAWP14225...... 178–179 LSKMAGH10...... 88–89 HTBP01300...... 182–183 JCWP01300...... 178–179 LSKMAGHDKIT...... 88–89 HTBP02500...... 182–183 JCWP02500...... 178–179 LSKMAGKP02...... 88–89 HTBP04700...... 182–183 JCWP04700...... 178–179 LSKMAGL10...... 88–89 HTTP01300...... 182–183 JCWP09025...... 178–179 LSKMAGLM02...... 88–89 HTTP02500...... 182–183 JCWP14225...... 178–179 LSKMAGN01...... 88–89 HTTP03700...... 182–183 JGWP01300...... 178–179 LSKMAGN04...... 88–89 HTTP04700...... 182–183 JGWP02500...... 178–179 LSKMAGS08...... 88–89 HTTP09030...... 182–183 JGWP04700...... 178–179 LSKMAGS15...... 88–89 HVHP01300...... 175–176 JGWP09025...... 178–179 LSKMAGT02...... 88–89 HVHP02500...... 175–176 JGWP14225...... 178–179 LSKMAGT10...... 88–89 HVHP04700...... 175–176 JHWP01300...... 178–179 LSKMPCR10...... 52–53 HVHP09050...... 175–176 JHWP02500...... 178–179 LSKMPCR50...... 52–53 HVHP14250...... 175–176 JHWP04700...... 178–179 LSKNF0500...... 55 HVLP00010...... 175–176 JHWP09025...... 178–179 LSKNS0500...... 55 HVLP01300...... 175–176 JHWP14225...... 178–179 LSKP09604...... 55 HVLP02500...... 175–176 JMWP01300...... 178–179 LSKP09624...... 55 HVLP04700...... 175–176, 197 JMWP02500...... 178–179 LSKPMRN30...... 55 www.merckmillipore.com 239 Catalogue No. Page Catalogue No. Page Catalogue No. Page LSKVBP100...... 55 MAWP037A0...... 137 MSNANLY50...... 55 LSWG02500...... 180 MAWP037AM...... 137 MSNU03010...... 52–53 LSWG04700...... 180 MAWP037P0...... 212 MSNU03050...... 52–53 LSWP01300...... 180 MAWP037PM...... 212 MSNUPSD50...... 55 LSWP02500...... 180 MDRLN0410...... 167 MSPHN6B10...... 165 LSWP03700...... 180 MELIPUNCH...... 35–36 MSPHN6B50...... 165 LSWP04700...... 180 MERS00002...... 30 MSPHNXB50...... 165 LSWP09025...... 180 MERSBAT01...... 30 MSPLRS096...... 35–36 LSWP14250...... 180 MERSSTX00...... 30 MSRLN0410...... 167 M000025A0...... 137 MERSSTX01...... 30 MSRLN0450...... 167 M00003700...... 137 MERSSTX03...... 30 MSSLBPC10...... 167 M000037A0...... 137 MERSSTX04...... 30 MSSLBPC50...... 167 M000037P0...... 139 MHWP037A0...... 137 MSTPCWH50...... 166 M04G-02-5PK...... 43–44 MIC230...... 43–44 MSVMHTS00...... 40–41, 52–53, 55 M04L-03-5PK...... 43–44 MMA125...... 41 MSVMHTS04...... 40–41 M04S-03-5PK...... 43–44 MMA130...... 41 MSVMHTS06...... 40–41 M8IPFRAME...... 35–36 MMA205...... 42 MSVMHTS07...... 40–41 M8IPS4510...... 35–36 MRCF0R030...... 100 MSVMHTS08...... 40–41 MACAC0RS5...... 24 MRCF0R100...... 100 MSVMHTS09...... 40–41 MACF09604...... 166 MRCF0R100ET...... 100 MSVMHTS0D...... 40–41 MACL09600...... 166 MRCPRT010...... 100 MSVMHTS0H...... 40–41 MACL09625...... 166 MSBVN1210...... 168 MSVMHTS10...... 40–41 MACL09645...... 166 MSBVN1250...... 168 MSVMHTSHV...... 40–41 MACL09680...... 166 MSBVN1B50...... 168 MVFCN1225...... 169 Appendix MACL0SC03...... 166 MSBVS1210...... 168 MVHVN4525...... 168 MACR08124...... 166 MSCP09600...... 166 MZFBN0W10...... 169 MACR08127...... 166 MSCPNPP00...... 167 MZFBN0W50...... 169 MACR81275...... 166 MSCPNPS00...... 167 MZFCN0W10...... 169 Index MADP19650...... 166 MSCPNUV40...... 167 MZFCN0W50...... 169 MAFBN0B50...... 169 MSDVN6510...... 168 MZHVN0W10...... 168 MAFCN0B50...... 169 MSDVN6550...... 168 MZHVN0W50...... 168 MAHAS4510...... 35–36 MSDVN6B50...... 168 MZPHN0W10...... 165 MAHFB1H60...... 169 MSFBN6B10...... 169 MZPHN0W50...... 165 MAHFC1H60...... 169 MSFBN6B50...... 169 NY1002500...... 184–185 MAIPS4510...... 35–36 MSFBNXB50...... 169 NY1004700...... 184–185 MAIPSWU10...... 35–36 MSFCN6B10...... 169 NY1009000...... 184–185 MAMCS0110...... 34, 38 MSFCN6B50...... 169 NY1100010...... 184–185 MAMCS9610...... 34, 38 MSFCNXB50...... 169 NY1102500...... 184–185 MAMIC3S10...... 34 MSGVN2210...... 168 NY1104700...... 184–185 MAMIC5S10...... 34 MSGVN2250...... 168 NY1109000...... 184–185 MAMIC8S10...... 34 MSGVN2B50...... 168 NY1H00010...... 184–185 MAMP09608...... 166 MSGVS2210...... 168 NY1H02500...... 184–185 MANM10010...... 38 MSHAS4510...... 35–36 NY1H04700...... 184–185 MANMN2010...... 38 MSHVN4510...... 168 NY1H09000...... 184–185 MANMN4010...... 38 MSHVN4550...... 168 NY2000010...... 184–185 MANMN6010...... 38 MSHVN4B50...... 168 NY2002500...... 184–185 MAPHN0B50...... 165 MSHVS4510...... 168 NY2004700...... 184–185 MATAHCL00...... 35–36 MSIPN4510...... 35–36 NY2009000...... 184–185 MATRNPS50...... 38 MSIPN4550...... 35–36 NY2H02500...... 184–185 MAUF01010...... 103 MSIPN4W50...... 35–36 NY2H04700...... 184–185 MAVM0960R...... 40–41 MSIPS4510...... 35–36 NY2H09000...... 184–185 MAWP025A0...... 137 MSIPS4W10...... 35–36 NY3002500...... 184–185 MAWP025AC...... 137 MSNANLY10...... 55 NY3004700...... 184–185

240 www.merckmillipore.com Catalogue No. Page Catalogue No. Page Catalogue No. Page NY3009000...... 184–185 PBTK07610...... 108–109 PIHT30R48...... 25 NY4100010...... 184–185 PBVK02510...... 108–109 PIMP12R48...... 25 NY4102500...... 184–185 PBVK04310...... 108–109 PIMP15R48...... 25 NY4104700...... 184–185 PBVK04710...... 108–109 PIMP30R48...... 25 NY4109000...... 184–185 PBVK06210...... 108–109 PIMWS0650...... 28–29 NY4H02500...... 184–185 PBVK07610...... 108–109 PIMWS1250...... 28–29 NY4H04700...... 184–185 PD1504700...... 213–214 PIMWS2450...... 28–29 NY4H09000...... 184–185 PEZCON0416...... 26 PIRP12R48...... 25 NY6000010...... 184–185 PEZGS0416...... 26 PIRP15R48...... 25 NY6002500...... 184–185 PEZGS0496...... 26 PIRP30R48...... 25 NY6004700...... 184–185 PEZGS0816...... 26 PISP12R48...... 25 NY6009000...... 184–185 PEZGS0896...... 26 PISP15R48...... 25 NY6H00010...... 184–185 PEZGUP0416...... 26 PISP30R48...... 25 NY6H02500...... 184–185 PEZXMSH01...... 26 PITP01250...... 25 NY6H04700...... 184–185 PFHYS0616...... 27 PIXP01250...... 25 NY6H09000...... 184–185 PFHYS1008...... 27 PLAC02510...... 108–109 NY8002500...... 184–185 PGN001-1EA...... 56 PLAC04310...... 108–109 NY8004700...... 184–185 PGN002-1EA...... 56 PLAC04710...... 108–109 NY8009000...... 184–185 PGN003-1EA...... 56 PLAC06210...... 108–109 NY8H02500...... 184–185 PGN004-1EA...... 56 PLAC07610...... 108–109 Appendix NY8H04700...... 184–185 PHCC0CLIP...... 31 PLAC09005...... 108–109 NY8H09000...... 184–185 PHCC20040...... 31 PLAC15005...... 108–109 P16938...... 144 PHCC20060...... 31 PLBC02510...... 108–109 P16940...... 144 PHCC40050...... 31 PLBC04310...... 108–109 PBCC02510...... 108–109 PHCC40500...... 31 PLBC04710...... 108–109

PBCC04310...... 108–109 PHCC60050...... 31 PLBC06210...... 108–109 Index PBCC04710...... 108–109 PHCC60500...... 31 PLBC07610...... 108–109 PBCC06210...... 108–109 PHCCBEADS...... 31 PLBC09005...... 108–109 PBCC07610...... 108–109 PHCCCABLE...... 31 PLBC15005...... 108–109 PBGC02510...... 108–109 PHCCP0WER...... 31 PLCC02510...... 108–109 PBGC04310...... 108–109 PHWP02500...... 172–174 PLCC04310...... 108–109 PBGC04710...... 108–109 PHWP04700...... 172–174 PLCC04710...... 108–109 PBGC06210...... 108–109 PHWP09025...... 172–174 PLCC06210...... 108–109 PBGC07610...... 108–109 PHWP14250...... 172–174 PLCC07610...... 108–109 PBHK02510...... 108–109 PI8P01250...... 25 PLCC09005...... 108–109 PBHK04310...... 108–109 PICL06P05...... 28–29 PLCC15005...... 108–109 PBHK04710...... 108–109 PICL24P05...... 28–29 PLGC02510...... 108–109 PBHK06210...... 108–109 PICM01250...... 25 PLGC04310...... 108–109 PBHK07610...... 108–109 PICM03050...... 25 PLGC04710...... 108–109 PBMK02510...... 108–109 PICM0RG50...... 25 PLGC06210...... 108–109 PBMK04310...... 108–109 PIDL06P05...... 28–29 PLGC07610...... 108–109 PBMK04710...... 108–109 PIDL24P05...... 28–29 PLGC09005...... 108–109 PBMK06210...... 108–109 PIEP12R48...... 25 PLGC15005...... 108–109 PBMK07610...... 108–109 PIEP15R48...... 25 PLHK02510...... 108–109 PBQK02510...... 108–109 PIEP30R48...... 25 PLHK04310...... 108–109 PBQK04310...... 108–109 PIFB06P05...... 28–29 PLHK04710...... 108–109 PBQK04710...... 108–109 PIFB24P05...... 28–29 PLHK06210...... 108–109 PBQK06210...... 108–109 PIHA01250...... 25 PLHK07610...... 108–109 PBQK07610...... 108–109 PIHA03050...... 25 PLHK09005...... 108–109 PBTK02510...... 108–109 PIHP01250...... 25 PLHK15005...... 108–109 PBTK04310...... 108–109 PIHP03050...... 25 PLTK02510...... 108–109 PBTK04710...... 108–109 PIHT12R48...... 25 PLTK04310...... 108–109 PBTK06210...... 108–109 PIHT15R48...... 25 PLTK04710...... 108–109 www.merckmillipore.com 241 Catalogue No. Page Catalogue No. Page Catalogue No. Page PLTK06210...... 108–109 RW1914250...... 186 SCWP14250...... 172–174 PLTK07610...... 108–109 S2EM004M99...... 35–36 SD1P014M04...... 203 PLTK09005...... 108–109 S384PCR10...... 52–53 SE1M003M00...... 13 PLTK15005...... 108–109 S384PCR50...... 52–53 SE1M179M6...... 13 PP2502500...... 188–189 SAMPHV001...... 164–165 SJFHM4710...... 198 PP2504700...... 188–189 SAMPHV004...... 164–165 SJHVM4710...... 198 PP4502500...... 188–189 SAMPLCR01...... 164–165 SJLHM4710...... 198 PP4504700...... 188–189 SAMPLCR04...... 164–165 SLAA025NB...... 160 PP4509030...... 188–189 SAMPLG001...... 164–165 SLAA025NK...... 160 PSET010R1...... 24 SAMPLG004...... 164–165 SLAA033SB...... 20–21 PSET010R5...... 24 SAMPSYSBL...... 164–165 SLAA033SS...... 20–21 PSHT004R1...... 24 SAMPSYSGR...... 164–165 SLAAV255F...... 20–21 PSHT004R5...... 24 SAVM38401...... 52–53 SLAP02550...... 20–21, 160 PSHT004S5...... 24 SC00B02RE...... 10–11 SLCR013NK...... 153–154 PSHT01R1...... 24 SC00B05RE...... 10–11 SLCR013NL...... 153–154 PSHT01R5...... 24 SC00B10RE...... 10–11 SLCR025NB...... 153–154 PSMT010R1...... 24 SC50FL025...... 13 SLCR025NK...... 153–154 PSMT010R5...... 24 SCGP00525...... 13 SLCR025NS...... 153–154 PSMW010R5...... 24 SCGPCAPRE...... 14 SLCRBZ5NK...... 162 PSRP004R1...... 24 SCGPS01RE...... 10–11 SLCRBZ5NZ...... 162 PSRP004R5...... 24 SCGPS02RE...... 10–11 SLCRDZ5NK...... 162 PSRP010R1...... 24 SCGPS05RE...... 10–11 SLCRDZ5NZ...... 162 PSRP010R5...... 24 SCGPT01RE...... 10–11 SLCRM25NK...... 163 PSST010R1...... 24 SCGPT02RE...... 10–11 SLCRM25NS...... 163 Appendix PSST010R5...... 24 SCGPT05RE...... 10–11 SLCRT13NL...... 153–154 PSSW010R5...... 24 SCGPT10RE...... 10–11 SLFA05000...... 160 PVC502500...... 135 SCGPU01RE...... 10–11 SLFA05010...... 160 PVC503700...... 135 SCGPU02RE...... 10–11 SLFG02550...... 160 Index PVC504700...... 135 SCGPU05RE...... 10–11 SLFG025LS...... 160, 206 QIA88...... 75–76 SCGPU10RE...... 10–11 SLFG025NB...... 155–156 RATF04700...... 172–174 SCGPU11RE...... 10–11 SLFG025NK...... 155–156 RATF14250...... 172–174 SCGVT05RE...... 10–11 SLFG025NS...... 155–156 RAWG02500...... 172–174 SCGVU01RE...... 10–11, 55 SLFG05000...... 160 RAWG0250C...... 172–174 SCGVU02RE...... 10–11 SLFG05010...... 160 RAWG04700...... 172–174 SCGVU05RE...... 10–11 SLFG55010...... 160 RAWP01300...... 172–174 SCGVU10RE...... 10–11 SLFG65000...... 160 RAWP02500...... 172–174 SCGVU11RE...... 10–11 SLFG65010...... 160 RAWP03700...... 172–174 SCHVU01RE...... 10–11 SLFG75000...... 160 RAWP04700...... 172–174 SCHVU02RE...... 10–11 SLFG75010...... 160 RAWP09025...... 172–174 SCHVU05RE...... 10–11 SLFG85000...... 160 RAWP14250...... 172–174 SCHVU11RE...... 10–11 SLFG85010...... 160 RNWP02500...... 184–185 SCNY00020...... 13 SLFGL25BS...... 160 RNWP04700...... 184–185 SCNY00040...... 13 SLFGR04NL...... 155–156 RTTP01300...... 182–183 SCNY00060...... 13 SLFGX13NK...... 155–156 RTTP02500...... 182–183 SCNY00100...... 13 SLFGX13NL...... 155–156 RTTP04700...... 182–183 SCR127...... 28–29 SLFGX13TL...... 155–156 RTTP14250...... 182–183 SCVPU02RE...... 10–11 SLFH025NB...... 155–156 RW0304700...... 186 SCVPU11RE...... 10–11 SLFH025NK...... 155–156 RW0309000...... 186 SCWP01300...... 172–174 SLFH025NS...... 155–156 RW0604700...... 186 SCWP0190R...... 172–174 SLFH05000...... 160 RW0609000...... 186 SCWP02500...... 172–174 SLFH05010...... 160 RW0614250...... 186 SCWP04700...... 172–174 SLFHR04NL...... 155–156 RW1904700...... 186 SCWP09025...... 172–174 SLFHX13NK...... 155–156

242 www.merckmillipore.com Catalogue No. Page Catalogue No. Page Catalogue No. Page SLFHX13NL...... 155–156 SLHNDZ5NK...... 162 SLLS025NS...... 155–156 SLFHX13TL...... 155–156 SLHNDZ5NZ...... 162 SLMP025SS...... 20–21 SLGL0250S...... 20–21 SLHNM25NK...... 163 SLMPL25SS...... 20–21 SLGN033NB...... 157–158 SLHNM25NS...... 163 SLPBDZ5NK...... 162 SLGN033NK...... 157–158 SLHNMZ5NZ...... 163 SLPBDZ5NZ...... 162 SLGN033NS...... 157–158 SLHNX13NK...... 157–158 SLSV025LS...... 20–21 SLGNDZ5NK...... 162 SLHNX13NL...... 157–158 SLSV025NB...... 152–153 SLGNDZ5NZ...... 162 SLHNX13TL...... 157–158 SLVV033RS...... 20–21 SLGNM25NK...... 163 SLHP033NB...... 159 SMWP01300...... 172–174 SLGNM25NS...... 163 SLHP033NK...... 159 SMWP02500...... 172–174, 214 SLGNX13NK...... 157–158 SLHP033NS...... 159 SMWP03700...... 172–174 SLGNX13NL...... 157–158 SLHP033RB...... 20–21 SMWP04700...... 172–174, 214 SLGNX13TL...... 157–158 SLHP033RS...... 20–21 SMWP09025...... 172–174 SLGP033NB...... 159 SLHPX13NK...... 159 SMWP14250...... 172–174 SLGP033NK...... 159 SLHPX13NL...... 159 SNAP2BHMD0100...... 119 SLGP033NS...... 159 SLHV004SL...... 20–21 SNAP2BHMN0100...... 119 SLGP033RB...... 20–21 SLHV013SL...... 20–21 SNAP2FRMD01...... 119 SLGP033RK...... 20–21 SLHV033NB...... 152–153 SNAP2FRMD02...... 119 SLGP033RS...... 20–21 SLHV033NK...... 152–153 SNAP2FRMN01...... 119 SLGP05010...... 160 SLHV033NS...... 152–153 SNAP2FRMN02...... 119 Appendix SLGPB5010...... 160 SLHV033RB...... 20–21 SNAP2MIDI...... 119 SLGPX13NK...... 159 SLHV033RK...... 20–21 SNAP2MINI...... 119 SLGPX13NL...... 159 SLHV033RS...... 20–21 SNAP2MM...... 119 SLGS02510...... 160 SLHVBZ5NK...... 162 SNAP2RL...... 119 SLGS025NB...... 160 SLHVBZ5NZ...... 162 SNAPABTR...... 119

SLGS033SB...... 20–21 SLHVDZ5NK...... 162 SPGPM10RJ...... 15 Index SLGS033SS...... 20–21 SLHVDZ5NZ...... 162 SPGPM20RJ...... 15 SLGSV255F...... 20–21 SLHVM25NK...... 163 SSWP01300...... 172–174 SLGV004SL...... 20–21 SLHVM25NS...... 163 SSWP02500...... 172–174 SLGV013SL...... 20–21 SLHVMZ5NZ...... 163 SSWP04700...... 172–174 SLGV033NB...... 152–153 SLHVR04NK...... 152–153 SSWP09025...... 172–174 SLGV033NK...... 152–153 SLHVR04NL...... 152–153 SSWP14250...... 172–174 SLGV033NS...... 152–153 SLHVX13NK...... 152–153 SVGP01015...... 16 SLGV033RB...... 20–21 SLHVX13NL...... 152–153 SVGP01050...... 16 SLGV033RK...... 20–21 SLHVX13TL...... 152–153 SVGPB1010...... 16 SLGV033RS...... 20–21 SLLG013SL...... 20–21 SVGPL10RC...... 16 SLGVR04NK...... 152–153 SLLG025SS...... 20–21 SVGV01015...... 16 SLGVR04NL...... 152–153 SLLGC13NL...... 156–157 SVGV010RS...... 16 SLGVS25PS...... 160 SLLGC25NS...... 156–157 SVGVB1010...... 16 SLGVS25US...... 160 SLLGDZ5NK...... 162 SVGVL10RC...... 16 SLGVS25XS...... 160 SLLGDZ5NZ...... 162 SVHV01015...... 16 SLGVX13NK...... 152–153 SLLGH13NK...... 153–154 SVHV010RS...... 16 SLGVX13NL...... 152–153 SLLGH13NL...... 153–154 SVHVB1010...... 16 SLGVX13TL...... 152–153 SLLGH25NB...... 153–154 SVHVL10RC...... 16 SLHA02510...... 160 SLLGH25NK...... 153–154 SVLP01300...... 175–176 SLHA025NB...... 160 SLLGH25NS...... 153–154 SVLP02500...... 175–176 SLHA033SB...... 20–21 SLLGM25NK...... 163 SVLP04700...... 175–176 SLHA033SS...... 20–21 SLLGM25NS...... 163 SVLP07550...... 175–176 SLHN033NB...... 157–158 SLLGR04NL...... 153–154 SVLP09050...... 175–176 SLHN033NK...... 157–158 SLLHC13NL...... 156–157 SVWG04700...... 175–176 SLHN033NS...... 157–158 SLLHC25NS...... 156–157 SX0001300...... 190, 205 SLHNBZ5NK...... 162 SLLHR04NK...... 153–154 SX0001301...... 190, 205 SLHNBZ5NZ...... 162 SLLHR04NL...... 153–154 SX0002500...... 190, 205 www.merckmillipore.com 243 Catalogue No. Page Catalogue No. Page Catalogue No. Page SX0002501...... 190, 205 UFC40W00...... 101–102 UFC910008...... 98–99 SX0004700...... 190, 205 UFC40W25...... 101–102 UFC910024...... 98–99 SX0004701...... 190, 205 UFC500308...... 98–99 UFC910096...... 98–99 SX00047RK...... 190, 205 UFC500324...... 98–99 VCTP01300...... 182–183 TCTP01300...... 182–183 UFC500396...... 98–99 VCTP02500...... 182–183 TCTP02500...... 182–183 UFC5003BK...... 98–99 VCTP04700...... 182–183 TCTP04700...... 182–183 UFC501008...... 98–99 VCTP14250...... 182–183 TCTP14250...... 182–183 UFC501024...... 98–99 VCWP01300...... 172–174 TETP01300...... 182–183 UFC501096...... 98–99 VCWP02500...... 172–174 TETP02500...... 182–183 UFC5010BK...... 98–99 VCWP04700...... 172–174 TETP04700...... 182–183 UFC503008...... 98–99 VCWP09025...... 172–174 TETP14250...... 182–183 UFC503024...... 98–99 VCWP14250...... 172–174 TMTP01300...... 182–183 UFC503096...... 98–99 VMWP01300...... 172–174 TMTP02500...... 182–183 UFC5030BK...... 98–99 VMWP02500...... 172–174 TMTP04700...... 182–183 UFC505008...... 98–99 VMWP04700...... 172–174 TMTP09030...... 182–183 UFC505024...... 98–99 VMWP09025...... 172–174 TMTP14250...... 182–183 UFC505096...... 98–99 VSWP01300...... 172–174 TP0001326...... 212 UFC5050BK...... 98–99 VSWP02500...... 172–174 TSTP01300...... 182–183 UFC510008...... 98–99 VSWP04700...... 172–174 TSTP02500...... 182–183 UFC510024...... 98–99 VSWP09025...... 172–174 TSTP04700...... 182–183 UFC510096...... 98–99 VSWP14250...... 172–174 TSTP14250...... 182–183 UFC5100BK...... 98–99 VVHP04700...... 175–176 TTTP02500...... 182–183 UFC700308...... 105 VVLP01300...... 175–176 TTTP04700...... 182–183 UFC701008...... 105 VVLP02500...... 175–176 Appendix UFC200324...... 98–99 UFC703008...... 105 VVLP04700...... 175–176 UFC201024...... 98–99 UFC710008...... 105 VVLP09050...... 175–176 UFC203024...... 98–99 UFC800308...... 98–99 VVLP14250...... 175–176 UFC205024...... 98–99 UFC800324...... 98–99 WBAVDCH01...... 121 Index UFC210024...... 98–99 UFC800396...... 98–99 WBAVDFL01...... 121 UFC30DV00...... 101–102 UFC801008...... 98–99 WBAVDP001...... 121 UFC30DV0S...... 101–102 UFC801024...... 98–99 WBKDS0025...... 121 UFC30DV25...... 101–102 UFC801096...... 98–99 WBKDS0100...... 121 UFC30GV00...... 101–102 UFC803008...... 98–99 WBKLS0050...... 121 UFC30GV0S...... 101–102 UFC803024...... 98–99 WBKLS0100...... 121 UFC30GV25...... 101–102 UFC803096...... 98–99 WBKLS0500...... 121 UFC30GVNB...... 101–102 UFC805008...... 98–99 WBLUC0100...... 122 UFC30HV00...... 101–102 UFC805024...... 98–99 WBLUC0500...... 122 UFC30HV25...... 101–102 UFC805096...... 98–99 WBLUF0100...... 122 UFC30HVNB...... 101–102 UFC810008...... 98–99 WBLUF0500...... 122 UFC30LG25...... 101–102 UFC810024...... 98–99 WBLUR0100...... 122 UFC30LH25...... 101–102 UFC810096...... 98–99 WBLUR0500...... 122 UFC30SV00...... 101–102 UFC900308...... 98–99 WP6110060...... 202 UFC30VV00...... 101–102 UFC900324...... 98–99 WP6111560...... 202 UFC30VV25...... 101–102 UFC900396...... 98–99 WP6122050...... 202 UFC40DV25...... 101–102 UFC901008...... 98–99 WP61MNT00...... 202 UFC40GV00...... 101–102 UFC901024...... 98–99 WP61RBD00...... 202 UFC40GV0S...... 101–102 UFC901096...... 98–99 WP6210060...... 202 UFC40GV25...... 101–102 UFC903008...... 98–99 WP6211560...... 202 UFC40HV00...... 101–102 UFC903024...... 98–99 WP6222050...... 202 UFC40HV25...... 101–102 UFC903096...... 98–99 WP62MNT00...... 202 UFC40LG25...... 101–102 UFC905008...... 98–99 WP62RBDNA...... 202 UFC40LH25...... 101–102 UFC905024...... 98–99 XF2004710...... 130 UFC40SV25...... 101–102 UFC905096...... 98–99 XF2004725...... 130

244 www.merckmillipore.com Catalogue No. Page Catalogue No. Page Catalogue No. Page XF2004755...... 130 XX1104705...... 132 XX4204709...... 193–194 XF2004756...... 130 XX1104706...... 132 XX4304700...... 192 XF2004758...... 130 XX1104707...... 190, 205 XX4304701...... 192–194 XF3001200...... 131 XX1104710...... 132 XX4304702...... 192 XF5423050...... 203 XX1104711...... 132 XX4304704...... 192, 206 XF5423055...... 203 XX1104715...... 190, 205 XX4304705...... 192 XF5423056...... 203 XX1105005...... 201 XX4304707...... 192 XFUF04701...... 107 XX11J4750...... 132 XX4404700...... 193–194 XFUF04711...... 107 XX1504700...... 127 XX4404702...... 193–194 XFUF07601...... 107 XX1504702...... 197 XX4404703...... 193–194 XFUF07611...... 107 XX1504705...... 197 XX4502500...... 193–194 XKEM00104...... 201 XX1504732...... 197 XX45025RK...... 193–194 XKEM00107...... 201 XX1604700...... 197 XX4504700...... 193–194 XT1200000...... 197 XX1604701...... 197 XX4504704...... 193–194 XX1002500...... 129 XX1604705...... 197 XX4504705...... 193–194 XX1002502...... 129 XX1604706...... 197 XX4504710...... 193–194 XX1002503...... 201 XX2004701...... 214 XX4504713...... 193–194 XX1002505...... 201 XX2004702...... 133 XX45047RK...... 193–194 XX1002508...... 201 XX2004703...... 133 XX5000000...... 138 XX1002514...... 129 XX2004704...... 133 XX5000002...... 138 Appendix XX1002530...... 129 XX2004708...... 127-128, 133 XX5000020...... 138 XX1002532...... 129 XX2004718...... 130 XX5002501...... 129-130 XX1002535...... 129-130 XX2004720...... 133 XX5004700...... 134 XX1002540...... 130 XX20047RK...... 133 XX5004710...... 134 XX1002542...... 130 XX2504700...... 133 XX5004740...... 134

XX1004700...... 128 XX2504705...... 194-195 XX5411560...... 203 Index XX1004702...... 128 XX2504735...... 133 XX6200004...... 138 XX1004703...... 130 XX2504755...... 140, 213 XX6200006P...... 200 XX1004704...... 197 XX2604735...... 137 XX6200035...... 201 XX1004705...... 201 XX2702509...... 140 XX6200036...... 201 XX1004707...... 127-128 XX2702510...... 190, 205 XX6300120...... 214 XX1004708...... 130 XX2702512...... 144 XX6300123...... 214 XX1004720...... 128 XX2702550...... 140 XX6403705...... 212 XX1004722...... 128 XX2702552...... 140 XX6403708...... 212 XX1004724...... 128 XX2702555...... 140 XX6403730...... 212 XX1004730...... 213 XX3001200...... 191 XX6403735...... 212 XX1004732...... 128 XX3001240...... 130 XX6403780...... 212 XX1004733...... 128 XX30012RK...... 191 XX6504704...... 214 XX1004744...... 201 XX3002500...... 191 XX6504707...... 214 XX1009000...... 127 XX3002502...... 141 XX6504708...... 214 XX1009002...... 127 XX3002510...... 141 XX6504709...... 214 XX1009003...... 127 XX3002514...... 191 XX6504710...... 214 XX1009004...... 127 XX3002567...... 144 XX6504713...... 214 XX1009010...... 127 XX30025RK...... 191 XX6504730...... 214 XX1009020...... 127 XX4002500...... 142 XX6602500...... 141, 213 XX1100000...... 205 XX40025RK...... 142 XX6602501...... 141 XX11000PP...... 140 XX4004700...... 196 XX6700010...... 144 XX1102012...... 201 XX4004704...... 196 XX6700024...... 144 XX1102503...... 201 XX4004714...... 196, 214 XX6700030...... 142, 144 XX1104700...... 131 XX4004716...... 196 XX6700034...... 192, 196 XX1104702...... 132 XX4004740...... 196 XX6700035...... 145 XX1104703...... 132 XX40047RK...... 196 XX6700059...... 204 XX1104704...... 132 XX4204708...... 193–195 XX6700104...... 144, 204 www.merckmillipore.com 245 Catalogue No. Page Catalogue No. Page Catalogue No. Page XX6700125...... 144 YT3009014...... 144 YY2214257...... 144-145, 195 XX6700P01...... 204 YT3009015...... 144 YY2214258...... 195 XX6700P05...... 204 YT3009016...... 144 YY2214265...... 145, 195 XX6700P10...... 204 YT30090HW...... 144 YY3009000...... 195 XX6700P20...... 204 YT3014257...... 145 YY3009053...... 195 XX6702500...... 142 YT30142HW...... 145 YY3009054...... 195 XX6702501...... 142 YT310RAHW...... 145 YY3014234...... 195 XX6702502...... 142 YY1301009...... 204 YY3014236...... 195 XX6702506...... 142 YY1301015...... 142, 144 YY3029307...... 195 XX6702507...... 142, 144 YY2004045...... 145, 195 YY3029366...... 195 XX6702508...... 142, 144 YY2004055...... 145, 195 ZTC04S008...... 92 XX67025RK...... 142 YY2004057...... 145 ZTC04S096...... 92 XX7100004...... 197 YY2004076...... 195 ZTC04S960...... 92 XX7104711K...... 213 YY2029348...... 142, 144 ZTC18M008...... 92 XX7104712...... 213 YY2209058...... 195 ZTC18M096...... 92 Y04C-02-5PK...... 43–44 YY2209059...... 195 ZTC18M960...... 92 Y04D-02-5PK...... 43–44 YY2209064...... 144 ZTC18S008...... 92 YT3009001...... 144 YY2209068...... 144 ZTC18S096...... 92 YT3009002...... 144 YY2214251...... 145, 195 ZTC18S960...... 92 YT3009003...... 144 YY2214252...... 145, 195 ZTSCXS008...... 92 YT3009013...... 144 YY2214253...... 145, 195 ZTSCXS096...... 92 Appendix Index by Products and Applications Index Description Page Description Page Description Page A C Cell cultureware CellASIC® ONIX microfluidic ADME assays �� 24-25, 167 Caco-2 assays �� 24-25 platform �� 43-46 Affinity purification ��80-84, 86-87, 90-91 Cell-based assays ECM coatings �� 28-29 Agarose ��63 Migration and angiogenesis assays �� 41-42 Millicell® inserts ��25 Agarose beads �� 83-87 Millicell® inserts ��25 Millicell® plates ��24 Amicon® Ultra filters ��98 Millicell® plates ��24 Multilayer flask ��27 Angiogenesis ��41 MultiScreen® filter plates ��34 Cellular analysis MultiScreen® filter plates CellASIC® ONIX microfluidic for Elispot �� 35-36 platform �� 43-46 B Scepter™ cell counter ��31 Flow cytometry ��47 Bead-based Transepithelial voltage measurement ��30 Imaging flow cytometry ��48 immunoassays ��123 Cell counter ��31 RNA detection in live cells ��64 Benzonase® nuclease ��74 Cell culture media sterilization Cell viability and proliferation assays ��34 Biochemicals ��62 Bottletop filter units ��10-12, 14-15 Centrifugal filters Blocking solution �� 121-122 Stericap™ filter units ��14 Amicon® Pro purification Blot stripping solution �� 121-122 Stericup® filter units �� 10-12 system �� 80-82 Bottletop filter units ��10-12, 14-15 Steriflip® filter units ��13 Amicon® Ultra filters �� 98-99 Buffer exchange ��80-81, 94-99, Sterile Millex® syringe filters �� 17-21 Centricon® filters ��105 102-103, 105-109 Steripak™ filter units ��15 Centrifree® filters ��104 Buffer filtration ��197 Steritop® filter units �� 10-11 Centriprep™ filters ��102 Buffers ��77 Cell culture reagents In vitro diagnostic use �� 98-99, 102, 104 BugBuster® protein extraction ECM coatings �� 28-29 Microcon® centrifugal ultrafilter ��100 reagents �� 71-72 Water ��10 Ultrafree® centrifugal filter �� 101-102

246 www.merckmillipore.com Description Page Description Page Description Page Chambered slide ��26 Swinnex® filter holders ��190 M Chemical compatibility tables ��220 Stainless steel syringe filter holders...... 191 Magnetic beads ��88-89 Chemotaxis assays ��34 Filters Chromatin immunoprecipitation ��66 Alpha particle monitoring ��136, 178-179 Manifolds 1225 Sampling manifold ��140 Chromatography Durapore® membrane filters...... 175 Chromatography columns �� 208-211 Mass spectrometry sample Asbestos monitoring ��137 Chromolith® column ��208 preparation ��181 EZ-Fit® filter holder manifolds...... 206 HPLC workflow solutions...... 148 Membrane filter discs...... 175-185 Samplicity® filtration system �� 164-165 Liquid-liquid extraction ��200 MF-Millipore™ membrane �� 172-174 Mass spectrometry �� 75-76 Mass spectrometry sample Prefilters �� 186-189 Mass spectrometry sample preparation ��92, 153-154, 181 Track-etched membranes �� 182-183 preparation �� 92 Millex® syringe filters..... 152-154, 156-158 Ultrafiltration discs �� 108-109 Matched weight filters ��139, 172-174 Millicup™ filter unit ��198 Flow cytrometry...... 47-48 Membranes (microfiltration) MilliSolve™ filtration system ��197 Fluid contamination analysis �� 212-214 Alpha particle monitoring ��136, 178-179 Mobile phase filtration ��197 Forceps ��200 Durapore® membrane filters...... 175 Multi-sample filtration �� 164-165 Fractionation �� 75-76 Mass spectrometry sample Samplicity® filtration system �� 164-165 preparation ��181 Separation of hydrophilic and polar Membrane filter discs...... 175-185 compounds ��211 G Membrane selection guide ��170 MF-Millipore™ membrane �� 172-174 Solid phase extraction ��199 Genomic DNA concentration ��51, 100 Prefilters �� 186-189 Appendix Thin layer chromatography ��211 Gravimetric analysis ��137, 212 Water ��215 Track-etched membranes �� 182-183 Chromatography columns �� 208-211 Membranes (ultrafiltration) Clarification ��101-102, 169 H Ultrafiltration discs �� 108-109 Ultrafiltration selection guide ��96 Competent cells �� 53-54 HPLC Microcon® centrifugal ultrafilter ��51, 100 Concentration ��80-81, 96-100, Chromatography columns �� 208-211 Index Microdialysis �� 172-174 102-103, 105-109 Chromolith® column ��208 Microscopy HPLC workflow solutions...... 148 CellASIC® ONIX microfluidic Liquid-liquid extraction ��200 D platform �� 43-46 Mass spectrometry sample Desalting ��92, 94-99, 102-103, 105-109 Cell growth ��26 preparation �� 153-154 Detergents ��77 Chambered slide ��26 Millex® syringe filters...... 152-153 Dialysis ��80-81, 95 Immunocytochemistry ��26 MilliSolve™ filtration system ��197 DNA purification ��51, 55, 100 Migration assays �� 34, 42 Mobile phase filtration ��197 Drug binding assays ��104 Millex® syringe filters Multi-sample filtration �� 164-165, Durapore® membrane �� 152-153, 175-176 Dissolution testing �� 162-163 168-169 Dualex™ Ultra transducer protector ...... 161 Samplicity® filtration system �� 164-165 Mass spectrometry sample E Separation of hydrophilic and preparation �� 153-154 Elispot �� 35-37 polar compounds ��211 Non-sterile Millex® Environmental Solid phase extraction ��199 syringe filters...... 152-160 monitoring ��see Particle monitoring Non-sterile Millex® syringe filter Enzyme assay plates ��165 I selection guide ��150 Sterile Millex® syringe filters ��17, 20-21 Immobilon® PVDF membrane ��115 F Sterile Millex® syringe filter Immunocytochemistry ��26 selection guide ��18 Filter holders In vitro diagnostic use �� 98-99, 102, 104 Transducer protectors...... 160-161 Epifluoresence filter holder ��131 Infrared spectrometer ��112, 114-115 Minicon® clinical sample concentrators ��104 Filter holder for batch filtration...... 196 Invasion assays ��34 Filter holder selection guide ��126 Monitors In-line filter holders...... 192-194 Asbestos monitoring ��137 Hydrosol™ filter holder ��133 L Fluid contamination analysis �� 212-214 Standing filter holders...... 195 Labeling reaction cleanup �� 80-81 Monolithic column ��208 Sterifil™ aseptic system �� 131-132 Lipids analysis ��112, 114-115 Multi-sample filtration ��164, 168-169 www.merckmillipore.com 247 Catalogue No. Page Catalogue No. Page Catalogue No. Page Multiwell plates PCR ��50 Steriflip® filter units ��13 Cell-based assays ��41 PCR cleanup �� 52-53 Steripak™ filter units ��15 ECM coatings �� 28-29 Pressure vessels �� 204-205 Steritop® filter units �� 10-11 Millicell® inserts ��25 Protein expression ��53-54, 59-60 Sterivex™ filter units ��16 Millicell® plates ��24 Protein extraction Stirred cells �� 105-109 MultiScreen® filter plate Automated protein purification...... 70 Syringe filters...... see Millex® syringe filters accessories ��166 DNase ��74 MultiScreen® filter plates ��34, 40-41, 167 From bacteria �� 71-72 MultiScreen® filter plates From mammalian cells ��72 T for Elispot �� 35-37 From yeast ��73 TCLP (Toxicity Test and Toxicity MultiScreen® filter plates for Lysozyme ��74 Characteristic Leaching Procedure) ��144 enzyme assays ��165 Phosphatase inhibitors �� 76-77 Transfection ��58 MultiScreen® filter plates with Protease inhibitors �� 76-77 Transporter assays �� 24-25 glass fiber ��169 Protein extraction reagent Triton®-free filters �� 172-174 MultiScreen® filter plates with selection guide ��68 nylon mesh ��38 ProteoExtract® and U MultiScreen® filter plates with ProteoEnrich™ kits �� 75-76 PVDF membrane ��168 Protein purification Ultrafiltration devices Multiwell plate selection guide ��218 Agarose beads ��83, 86-87 Amicon® Ultra selection guide ��97 Purification ��103 Agarose beads selection guide ��84 Ultrafiltration device selection guide ��96 Vacuum manifold �� 39-41 Amicon® Pro purification Urine and CSF concentration ��104 system �� 80-82 Automated protein purification...... 70 V N Cleavage of fusion tags �� 90-91 Appendix Vacuum flask ��201 NGS library preparation ��56 Magnetic beads �� 88-89 Vacuum pumps �� 201-203 PureProteome™ magnetic beads �� 88-89 P Protein quantitation ��114-115, 123 PureProteome™ magnetic beads �� 88-89

Index W PAMPA assays �� 24-25 Western blotting products Particle monitoring Antibodies ��124 1225 Sampling manifold ��140 Q ECL substrates �� 121-122 Aerosol analysis accessories...... 138 Quartz filters �� 187-188 Immobilon® PVDF membrane �� 115-117 Alpha particle PVDF �� 115-117 monitoring ��136, 178-179 SNAP i.d.® system �� 118-120 Asbestos monitoring ��137 S Whole cell incubation assays ��34 Aviation fluids monitoring �� 212-213 Solubility testing ��167 Whole organism screening ��38 Environmental monitoring �� 144-145 Solvent filtration ��197 Filterjet™ solvent dispenser ��142 Sterile filtration Fluid contamination analysis �� 212-214 Bottletop filter units ��10-12, 14-15 Z Hydraulic fluids analysis ��214 Millex® syringe filters ��17, 20-21 ZipTip® pipette tips ��92 Solvent filter dispenser ��141 Stericap™ filter units ��14 Zero-head extraction...... 144 Stericup® filter units �� 10-12

248 www.merckmillipore.com How to Place an Order When ordering, please indicate the catalogue number for each product so that we can process your order quickly. For a complete global office listing with street addresses, please visit:www.merckmillipore.com/offices E-Commerce: The products in this publication are available on-line, along with pricing and availability.

Customer Service Contact Information Country Phone Fax Email Austria 0820 874 464 0820 874 444 [email protected] Belgium 070 22 56 45 070 22 56 44 [email protected] Denmark 70 10 56 45 70 10 56 44 [email protected] Finland 020 305 645 020 305 644 [email protected] France 0825 045 645 0825 045 644 [email protected] Germany 01805 045 645 01805 045 644 [email protected] Ireland 1890 924 645 1890 924 884 [email protected] Italy 848 845 645 848 845 644 [email protected]

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Technical Service Contact Information Country Phone Language Email Country Phone Language Email France 01 30 12 70 70 French, [email protected] China 400 889 1988 Mandarin, asiatechserv@ Switzerland FR 021 641 25 56 English (incl. Hong Kong) (China) Korean, merckgroup.com English Belgium FR 02 686 08 98 Korea Germany 06196 494 299 German, [email protected] Taiwan Switzerland GE 043 399 40 49 English Singapore Austria 0187 789 2642 Other Asia/Pacific UK 01923 813 365 English [email protected] Japan 0120 633 358 Japanese, bioinfo@

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To Place an Order or Receive Technical Assistance In Europe, please call Customer Service: France: 0825 045 645 Germany: 01805 045 645 Italy: 848 845 645 Spain: 901 516 645 Option 1 Switzerland: 0848 645 645 United Kingdom: 0870 900 4645 For other countries and additional contact information, please see Page 249.

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This catalog supersedes all other catalogs and product claims. Merck Millipore, 293-Free, AbSurance, Biopore, ChIPAb+, Cytobuster, Direct Stack, D-Tube, Dualex, Durapel, Easycyte, Ekapture, Filterjet, Fluoropore, GigaSingles, GST•Bind, GST•Mag, GST•Tag, His•Mag, Hydrosol, Isopore, KOD Xtreme, Luminata, Lysonase, MF-Millipore, Millicoat, Millicup, MilliSolve, Millivac, Mitex, NucBuster, Omnipore, Origami, Overnight Express, OxyBlot, OxyELISA, OxyICC, PhosphoSafe, ProteoEnrich, ProteoJuice, PureEpi, PureGenome, PureProteome, ReBlot, Reportasol, Reput(Ab)le, RiboJuice, rLysozyme, Rosetta-gami, Scepter, Singles, SmartFlare, Solvinert, SpinPrep, S•Tag, Stericap, Steripak, Sterivac, Sterivex, Tag•off, Xarrest, Yeastbuster and the M Mark are trademarks of Merck KGaA, Darmstadt, Germany. Amicon, Amnis, Benzonase, Bio-Pak, bløk, BugBuster, Calbiochem, Cathivex, CellASIC, Centricon, Centriflo, Centrifree, Centriplus, Centriprep, Chemicon, Chromolith, Direct Detect, Durapore, Extrelut, EZ-Fit, EZ-Stream, FlowSight, GeneJuice, Guava, His•Tag, His•Bind, ImageStream, Immobilon, Lichrolut, LiChrospher, Magna ChIP, Microcon, Millex, Samplicity, Millex, Millicell, Milliplex, Millipore Express, Milli-Q, Minicon, Montage, MultiScreen, Muse, Nanojuice, NovaCHOice, Novagen, Novatag, OmniPur, PopCulture, PROSEP, ProteoExtract, Purospher, Samplicity, SeQuant, SNAP i.d., Stericup, Sterifil, Steriflip, Steritop, Strep•Tactin, Strep•Tag, Swinnex, T7•Tag, UCOE, Ultracel, Ultrafree, ULTROL, Upstate, ZIC, ZipTip and Zwittergent are registered trademarks of Merck KGaA, Darmstadt, Germany. Trademarks belonging to third parties are the properties of their respective owners. Lit No. CA5833ENEU BS-GEN-13-08849 01/2014 Printed in the USA. © 2014 EMD Millipore Corporation, Billerica MA USA. All rights reserved.