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Macrobrachium Rosenbergii Ramachandra Naik a T, Shivananda Murthy H and Anjanayappa H N College of Fisheries, India

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Macrobrachium Rosenbergii Ramachandra Naik a T, Shivananda Murthy H and Anjanayappa H N College of Fisheries, India conferenceseries.com 918th Conference 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain Workshop Day 1 Aqua Europe 2017 Page 29 Federico G Witt, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain Federico G Witt AlgaEnergy S A, Spain Nannochloropsis limnetica (Eustigmatophyceae): A new freshwater microalga for rotifer feed in marine hatcheries nert feeds are available in the aquaculture market for mass production and enrichment of the rotifer Brachionus plicatilis. INevertheless, microalgae are still the preferred feed. Although good rotifer growth rates can be achieved with the freshwater Chlorella, marine strains of Nannochloropsis are more suitable due to their higher content in omega-3 PUFAs, mainly EPA. In this work, the growth of the freshwater species Nannochloropsis limnetica at different temperatures in semi-continuous cultures was studied. N. limnetica could be cultured in the range between 15°C and 27°C with highest dry-weight productivities at 22°C. When compared with N. gaditana in semi-continuous cultures at laboratory scale, productivities of both species were similar, reaching 0.64 g l−1 day−1. Moreover, the nutritional values for B. plicatilis of N. limnetica and N. gaditana were compared. Regarding FA, both species showed similar profiles. In laboratory-scale cultures with high algal rations, B. plicatilis concentrations and egg ratios of the rotifer cultured with N. limnetica doubled those attained with identical doses of N. gaditana, while maintaining the same FA profile in the filter-feeder. These results proved the potential of this freshwater species for marine aquaculture. In hatchery-scale experiments in which B. plicatilis was fed with baker's yeast supplemented either with on-site produced fresh microalgal cultures or with concentrated algae, similar growth results were obtained for both microalgal species in 5-day batch cultures, although higher dry weights and slightly better egg ratios were observed with the freshwater strain. Our results demonstrate the potential of the freshwater N. limnetica as a substitute of Chlorella in live- feed production protocols due to its better FA profile. Moreover,N. limnetica promotes higher growth in the rotifer Brachionus plicatilis than its relative marine N. gaditana. Additionally, no potentially pathogenic marine bacteria would be present in cultures of the freshwater species in comparison to its marine counterparts. Biography Federico G Witt has over 25 years’ experience on microalgae research. Currently, he is the Production Director of AlgaEnergy, a Spanish Biotech aimed to the commer- cialization of microalgae biomass and its derivatives that has been recently selected by the European Commission as one of the SMEs with the highest growth potential in Europe. He manages two production plants and carries out R&D activities on the development of new microalgae-based products. Formerly, he was R&D Director of Blue Water Solutions, where he focused on the development and implementation of a novel waste water treatment system that successfully combined a helophyte hydroponic culture with filamentous green algae. Moreover, he has held positions at the Spanish Scientific Research Council and some universities in Spain and Germany. His main research lines were cryptochrome, membrane transport systems, protein biochemistry, C and N plant metabolisms and photosynthesis. [email protected] Notes: J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017 Page 30 Shengkang Li, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain Shengkang Li Shantou University, China Tumor necrosis factor receptor-associated factor 6 (TRAF6) from mud crab participates in anti- lipopolysaccharide factors (ALFs) gene expression umor necrosis factor receptor-associated factor 6 (TRAF6) is a cytoplasm key signal adapter protein that mediates signals Tactivated by tumor necrosis factor receptor (TNFR) superfamily and the Interleukin-1 receptor/Toll-like receptor (IL-1/ TLR) superfamily. The full-length 2492 bp Scylla paramamosain TRAF6 (Sp-TRAF6) contains a 1800 bp of open reading frame (ORF) encoding 598 amino acids, including an N-terminal RING-type zinc finger, two TRAF-type zinc fingers and a conserved C-terminal meprin and TRAF homology (MATH) domain. Multiple alignment analysis shows that the putative amino acid sequence of Sp-TRAF6 has highest identity with Pt-TRAF6 (KP341006) from Portunus trituberculatus at 88%, while the similarity of other crustacea sequences was 54-55%. RT-PCR results indicated that the Sp-TRAF6 transcripts were predominantly expressed in the hepatopancreas and stomach, whereas it was barely detected in the heart and hemocytes in our study. Further, Sp-TRAF6 transcritripts were significantly up-regulated after immune challenge with Vibrio parahemolyticus or LPS. Our previous study had characterized two novel anti-lipopolysaccharide factor isoforms from S.paramamosain (SpALF5 and SpALF6). Both of them contain a conserved LPS-binding domain with two conservative cysteine residues, which is critical for their antimicrobial function. The vitro binding and antimicrobial activity assays indicated that the recombinant SpALF5 and SpALF6 protein generated from prokaryotic expression system showed a varying degree of binding activity towards bacteria and fungus, and exhibited a broad spectrum of antimicrobial activities against Gram-positive, Gram-negative bacterium and fungi. Therefore, six ALF isoforms from mud crab had been reported up to now. To investigate Sp-TRAF6 activating SpALFs gene expression, RNA interference assay was carrried out to examine the mRNA level of six SpALFs after silencing Sp-TRAF6 gene. The results showed that silencing Sp-TRAF6 gene could inhibit SpALF1, SpALF2, SpALF5 and SpALF6 expression in hemocytes, while SpALF1, SpALF3, SpALF4, SpALF5 and SpALF6 in hepatopancreas. Taken together, the acute-phase response to immune challenges and the inhibition of SpALFs gene expression indicate that Sp-TRAF6 plays an important role in host defense against pathogen invasion via regulation of ALF gene expression in S. Paramamosain. Biography Shengkang Li has completed his PhD from Sun Yat-sen University and Post-doctoral studies from IFREMER Centre de Nantes, France. He is the Principle Investigator of marine micro-organisms research group in Marine Biology Institute, Shantou University. He has published more than 30 papers in reputed journals and has been serving as a reviewer for many reputed journals. [email protected] Notes: J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017 Page 31 conferenceseries.com 918th Conference 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain Scientific Tracks & Abstracts Day 1 Aqua Europe 2017 Page 33 Luis Lagos et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain Experimental advances in shellfish offshore zone production in Basque Country (SE Bay of Biscay) Luis Lagos, Leire Arantzamendi, Izaskun Zorita, Oihana Solaun, Marta Revilla, Oihane Muñoz and Ibon Galparsoro AZTI, Spain he Basque Government expressed the need of the establishment of an offshore zone for the expansion of aquaculture production Tin the Basque Country (Aquaculture Development Strategic Plan 2014-2020). For that, AZTI made the site selection as well as the oceanographic and microbiological, sanitary characterization and monitoring for the declaration of a “Shellfish Zone Production” (SZP). The offshore SZP has the minimum requisites to assert; among others: • The existing environmental and regulatory compliance. According to the measurement of organic and metal elements concentration, shellfish culture is enabled at SZP. In addition, according to the microbiological data, the produced shellfish does not require depuration for commercialization. • Good Oceanographic conditions. (T = 11.5-23.5 ºC; current speed = 18. 7 ± 4.6 cm seg-1; wave high = 2± 1.4 m; Salinity = 33-35.8 PSU); chlorophyll “a” concentration = 0-10 µg L-1 with dominance of diatoms during blooms. • The design and validation of strength of submerged longline structures proposed for use in ZPM open waters (image 1). The culture technology chosen is described. The shellfish culture model of SZP exerts low impact over the marine ecosystem. • Good culture conditions (growth and survival of experimentally cultured species). The aquaculture performance of mussel Mytilus galloprovincialis reveals that the growth patterns (daily specific rates (%), length = 0.32; shell weight = 0.82-0.84; Dry meat weight = 0.95-1.00) are similar to those observed in others productions sites in Spain and Europe. • The minimum conflicts with fishing activity and other maritime sectors. The SZP is an example for development of offshore aquaculture in Atlantic arc and Maritime spatial planning. The research leading to these results has been undertaken as part of the AquaSpace project (Ecosystem Approach to making Space for Aquaculture, http://aquaspace-h2020.eu) and has received funding from the
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