conferenceseries.com 918th Conference
5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Workshop Day 1
Aqua Europe 2017
Page 29 Federico G Witt, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Federico G Witt AlgaEnergy S A, Spain
Nannochloropsis limnetica (Eustigmatophyceae): A new freshwater microalga for rotifer feed in marine hatcheries nert feeds are available in the aquaculture market for mass production and enrichment of the rotifer Brachionus plicatilis. INevertheless, microalgae are still the preferred feed. Although good rotifer growth rates can be achieved with the freshwater Chlorella, marine strains of Nannochloropsis are more suitable due to their higher content in omega-3 PUFAs, mainly EPA. In this work, the growth of the freshwater species Nannochloropsis limnetica at different temperatures in semi-continuous cultures was studied. N. limnetica could be cultured in the range between 15°C and 27°C with highest dry-weight productivities at 22°C. When compared with N. gaditana in semi-continuous cultures at laboratory scale, productivities of both species were similar, reaching 0.64 g l−1 day−1. Moreover, the nutritional values for B. plicatilis of N. limnetica and N. gaditana were compared. Regarding FA, both species showed similar profiles. In laboratory-scale cultures with high algal rations, B. plicatilis concentrations and egg ratios of the rotifer cultured with N. limnetica doubled those attained with identical doses of N. gaditana, while maintaining the same FA profile in the filter-feeder. These results proved the potential of this freshwater species for marine aquaculture. In hatchery-scale experiments in which B. plicatilis was fed with baker's yeast supplemented either with on-site produced fresh microalgal cultures or with concentrated algae, similar growth results were obtained for both microalgal species in 5-day batch cultures, although higher dry weights and slightly better egg ratios were observed with the freshwater strain. Our results demonstrate the potential of the freshwater N. limnetica as a substitute of Chlorella in live- feed production protocols due to its better FA profile. Moreover,N. limnetica promotes higher growth in the rotifer Brachionus plicatilis than its relative marine N. gaditana. Additionally, no potentially pathogenic marine bacteria would be present in cultures of the freshwater species in comparison to its marine counterparts. Biography Federico G Witt has over 25 years’ experience on microalgae research. Currently, he is the Production Director of AlgaEnergy, a Spanish Biotech aimed to the commer- cialization of microalgae biomass and its derivatives that has been recently selected by the European Commission as one of the SMEs with the highest growth potential in Europe. He manages two production plants and carries out R&D activities on the development of new microalgae-based products. Formerly, he was R&D Director of Blue Water Solutions, where he focused on the development and implementation of a novel waste water treatment system that successfully combined a helophyte hydroponic culture with filamentous green algae. Moreover, he has held positions at the Spanish Scientific Research Council and some universities in Spain and Germany. His main research lines were cryptochrome, membrane transport systems, protein biochemistry, C and N plant metabolisms and photosynthesis.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 30 Shengkang Li, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Shengkang Li Shantou University, China
Tumor necrosis factor receptor-associated factor 6 (TRAF6) from mud crab participates in anti- lipopolysaccharide factors (ALFs) gene expression umor necrosis factor receptor-associated factor 6 (TRAF6) is a cytoplasm key signal adapter protein that mediates signals Tactivated by tumor necrosis factor receptor (TNFR) superfamily and the Interleukin-1 receptor/Toll-like receptor (IL-1/ TLR) superfamily. The full-length 2492 bp Scylla paramamosain TRAF6 (Sp-TRAF6) contains a 1800 bp of open reading frame (ORF) encoding 598 amino acids, including an N-terminal RING-type zinc finger, two TRAF-type zinc fingers and a conserved C-terminal meprin and TRAF homology (MATH) domain. Multiple alignment analysis shows that the putative amino acid sequence of Sp-TRAF6 has highest identity with Pt-TRAF6 (KP341006) from Portunus trituberculatus at 88%, while the similarity of other crustacea sequences was 54-55%. RT-PCR results indicated that the Sp-TRAF6 transcripts were predominantly expressed in the hepatopancreas and stomach, whereas it was barely detected in the heart and hemocytes in our study. Further, Sp-TRAF6 transcritripts were significantly up-regulated after immune challenge with Vibrio parahemolyticus or LPS. Our previous study had characterized two novel anti-lipopolysaccharide factor isoforms from S.paramamosain (SpALF5 and SpALF6). Both of them contain a conserved LPS-binding domain with two conservative cysteine residues, which is critical for their antimicrobial function. The vitro binding and antimicrobial activity assays indicated that the recombinant SpALF5 and SpALF6 protein generated from prokaryotic expression system showed a varying degree of binding activity towards bacteria and fungus, and exhibited a broad spectrum of antimicrobial activities against Gram-positive, Gram-negative bacterium and fungi. Therefore, six ALF isoforms from mud crab had been reported up to now. To investigate Sp-TRAF6 activating SpALFs gene expression, RNA interference assay was carrried out to examine the mRNA level of six SpALFs after silencing Sp-TRAF6 gene. The results showed that silencing Sp-TRAF6 gene could inhibit SpALF1, SpALF2, SpALF5 and SpALF6 expression in hemocytes, while SpALF1, SpALF3, SpALF4, SpALF5 and SpALF6 in hepatopancreas. Taken together, the acute-phase response to immune challenges and the inhibition of SpALFs gene expression indicate that Sp-TRAF6 plays an important role in host defense against pathogen invasion via regulation of ALF gene expression in S. Paramamosain. Biography Shengkang Li has completed his PhD from Sun Yat-sen University and Post-doctoral studies from IFREMER Centre de Nantes, France. He is the Principle Investigator of marine micro-organisms research group in Marine Biology Institute, Shantou University. He has published more than 30 papers in reputed journals and has been serving as a reviewer for many reputed journals.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 31 conferenceseries.com 918th Conference
5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Scientific Tracks & Abstracts Day 1
Aqua Europe 2017
Page 33 Luis Lagos et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Experimental advances in shellfish offshore zone production in Basque Country (SE Bay of Biscay) Luis Lagos, Leire Arantzamendi, Izaskun Zorita, Oihana Solaun, Marta Revilla, Oihane Muñoz and Ibon Galparsoro AZTI, Spain
he Basque Government expressed the need of the establishment of an offshore zone for the expansion of aquaculture production Tin the Basque Country (Aquaculture Development Strategic Plan 2014-2020). For that, AZTI made the site selection as well as the oceanographic and microbiological, sanitary characterization and monitoring for the declaration of a “Shellfish Zone Production” (SZP). The offshore SZP has the minimum requisites to assert; among others: • The existing environmental and regulatory compliance. According to the measurement of organic and metal elements concentration, shellfish culture is enabled at SZP. In addition, according to the microbiological data, the produced shellfish does not require depuration for commercialization. • Good Oceanographic conditions. (T = 11.5-23.5 ºC; current speed = 18. 7 ± 4.6 cm seg-1; wave high = 2± 1.4 m; Salinity = 33-35.8 PSU); chlorophyll “a” concentration = 0-10 µg L-1 with dominance of diatoms during blooms. • The design and validation of strength of submerged longline structures proposed for use in ZPM open waters (image 1). The culture technology chosen is described. The shellfish culture model of SZP exerts low impact over the marine ecosystem. • Good culture conditions (growth and survival of experimentally cultured species). The aquaculture performance of mussel Mytilus galloprovincialis reveals that the growth patterns (daily specific rates (%), length = 0.32; shell weight = 0.82-0.84; Dry meat weight = 0.95-1.00) are similar to those observed in others productions sites in Spain and Europe. • The minimum conflicts with fishing activity and other maritime sectors. The SZP is an example for development of offshore aquaculture in Atlantic arc and Maritime spatial planning. The research leading to these results has been undertaken as part of the AquaSpace project (Ecosystem Approach to making Space for Aquaculture, http://aquaspace-h2020.eu) and has received funding from the European Union's Horizon 2020 Framework Programme for Research and Innovation under grant agreement n° 633476.
Biography Luis Lagos is an Aquaculture Professional, PhD for Pontificia Universidad Católica de Valparaíso, Chile with 18 years of experience in intensive fish production. He is involved in the aquaculture optimization and expansion. He gives counseling for problems related to the reproduction of fish and shellfish, as well as continuous improvement of production. Actually he works in Marine Investigation in AZTI, with main objectives to promote aquaculture development in Biscay Bay and Basque Country.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 35 Claudia Grimm et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Effect of resveratrol and genistein on ∆6-desaturase expression and protein levels in Rainbow trout Claudia Grimm1, Stefanie Staats2, Gerald Rimbach2 and Carsten Schulz1 1Gesellschaft fuer Marine Aquakultur mbH, Germany 2University of Kiel, Kiel, Germany
n order to spare natural marine resources, namely fish oil and fish meal, a general trend in aquaculture is the inclusion of alternative Iplant based products for fish nutrition. Especially fatty acid (FA) composition of plant oils differ substantially from fish oil. To generate more sustainable fish feed but guarantee an end product rich in omega-3 (n-3) FA, the combination of plant oils and phytochemicals in fish feeds is promising and was used in this study. Resveratrol (RV) and genistein (G) are two phytochemicals that, in in vitro experiments, interact with ∆6-desaturase, the key enzyme in FA metabolism, and function as ligand for PPARα, a transcription factor involved in β-oxidation of fatty acids. Rainbow trout (36.35±0.03 g) were fed 9 diets with reduced fish oil levels (F4=4%, F2=2% and F0=0%) and inclusion of either RV (F4+RV, F2+RV and F0+RV) or G (F4+G, F2+G and F0+G) for 8 weeks. Both, RV and G affected FA composition of whole fish. 20:5 n-3 (EPA) and 22:6 n-3 (DHA) were significantly increased whereas 18:2 n-6 (LA) and 18:3 n-3 (ALA) were significantly reduced in fish fed the more vegetable diets F2 and F0 supplemented with RV. At the same time, protein levels of ∆6-desaturase were significantly elevated in livers of fish fed diets supplemented with RV, especially F0+RV. mRNA expression of ∆6-desaturase, PPARα and other target genes were significantly influenced by different fish oil levels but not by addition of RV or G. We were able to show in vivo that RV and G lead to increased ∆6-desaturase enzyme level and further to elevated EPA and DHA levels in the fish. This study shows the potential for using especially RV in aquaculture diets low in fish oil and highlights the molecular mechanisms behind the regulation of FA synthesis under the influence of RV or G.
Biography Claudia Grimm has background in Biology with a focus on Aquatic Ecology. She is working in the current project in the Field of Fish Nutrition in Aquaculture. She works on classical questions concerning fish nutrition and also up to date trends like the reduction of marine resources in fish feeds. She covers all steps of nutritional research: diet formulation, diet production, feeding trial, sampling, sample preparation and analyses. She investigates molecular mechanisms behind promising approaches as well as the in vivo application. The combination of in vivo and in vitro experiments is of special interest to her.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 36 Ramachandra Naik A T et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Sardine oil as a source of lipid in the diet of giant freshwater prawn, Macrobrachium rosenbergii Ramachandra Naik A T, Shivananda Murthy H and Anjanayappa H N College of Fisheries, India
he freshwater prawn, Macrobrachium rosenbergii is a more popular crustacean cultured widely in monoculture system in India. TIt has got high nutritional value in the human diet. Hence, understanding its enzymatic and body composition is important in order to judge its flesh quality. Fish oil specially derived from Indian oil Sardine is a good source of highly unsaturated fatty acid and lipid source in fish/prawn diet. A 35% crude protein diet with graded levels of Sardine oil as a source of fat was incorporated at four levels viz, 2.07, 4.07, 6.07 and 8.07% maintaining a total lipid level of feed at 8.11, 10.24, 12.28 and 14.33% respectively. Diet without Sardine oil (6.05% total lipid) was served as basal treatment. The giant freshwater prawn, Macrobrachium rosenbergii was used as test animal and the experiment lasted for 112 days. Significantly higher gain in weight of prawn was recorded in the treatment with 6.07% Sardine oil incorporation followed by higher specific growth rate, food conversion rate and protein efficiency ratio. The 8.07% Sardine oil diet produced the highest RNA:DNA ratio in the prawn muscle. Digestive enzyme analyses in the digestive tract and mid-gut gland showed the greatest activity in prawns fed with 8.07% diet.
Biography Ramachandra Naik A T is working as an Associate Professor at College of Fisheries, Mangalore, Karnataka, India.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 37 Mohammad Gorgij Jaski et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Determination of effective parameters on growth rate and protein content of Spirulina platensis under laboratory condition Mohammad Gorgij Jaski1 and Kiuomars Rohani-Ghadikolaei2 1Islamic Azad University, Iran 2Persian Gulf and Oman Sea Ecological Research Institute, Iran
he major nutritional interest in Spirulina is due to its high protein content, ease of digestion and a significant content of vitamins, Tminerals, amino acids and high value pigments, which have applications in health foods, feed and therapeutic industries. In order to provide optimum growth and protein content, S. platensis cells were grown in modified Zarrouk (1966) media culture at different salinity (30, 32 and 35 ppt), temperature (20, 23, 26 and 28°C) and intensity (50, 75 and 90 µmol -2m s-1). The results of the present study clearly showed that the highest alga biomass and growth rate was obtained following culture under 32 ppt salinity, 26°C temperature, and under a 13 h light:11 h dark photoperiod regime at a light intensity of 90 µmol m-2s-1 provided by cool white fluorescent tubes. Maximum alga biomass and growth rate of S. platensis in a 5 liter Erlenmeyer flask for 12 days reached to 8 gr L−1 and 0.28 day-1, respectively. However, the mean alga biomass of S. platensis in temperature regimes 20, 23, 26 and 28ºC (under salinity of 32 ppt, and 75 µmol m-2s-1 irradiance) were 4.72, 5.15, 7.69 and 7.03 gr L−1, respectively. The results clearly showed that S. platensis successfully cultivated under different physical conditions and maximum protein content was produced in 30 ppt salinity, 28°C temperature, and 75 µmol m-2s-1 irradiance. A two-way ANOVA indicated significant effects of temperature on the growth rate of S. platensis followed by salinity, and then the interaction between temperature and salinity (p<0.05). Based on the results from the present study, providing suitable media culture and physical condition can be considered as a promising method to S. platensis cultivation for achieving optimal biomass and protein production.
Biography Mohammad Gorgij Jaski has completed his MSc in Aquaculture from Islamic Azad University, Iran and currently he is a PhD student of Aquaculture Nutrition. He is the Head of shrimp hatchery production named, Sontderaf in Jask, Iran. He has published more than 2 papers in reputed journals and presented more than 3 papers in international conferences.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 38 Samy Yehya El-Zaeem et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Evaluation of the productive performance characteristics of Nile tilapia, Oreochromis niloticus under the effect of DNA transfer Samy Yehya El-Zaeem1 and Rasha Ismael Elwan2 1Alexandria University, Egypt 2National Institute of Oceanography and Fisheries, Egypt
his study was conducted to compare and evaluate the productive performance characteristics of the base generation (F0) of Nile Ttilapia, Oreochromis niloticus under the effect of introducing a fragmented purified DNA isolated from Blue tilapia, O. aureus or common carp, Cyprinus carpio into the gonads of O. niloticus parent. The results showed that daily gain of genetically modified O. niloticus treated with carp DNA improved significantly (P≤0.05) compared to purebreds. Also, the same treatment had significant (P≤0.05) superiority for SGR%/day compared to purebreds. The highest mean value of protein content in fish (57.55%) was obtained by Nile tilapia received carp DNA, but did not differ significantly (P≤0.05) from that of purebred of Nile tilapia (57.31%). In addition, Nile tilapia received carp DNA had significant superiority (P≤0.05) in lipid content, but did not differ significantly from those of Nile tilapia received Blue tilapia DNA. The best or higher mean of FCR and PER (2.04±0.02 and 1.53±0.01, respectively) were achieved by Nile tilapia received carp DNA, but did not differ significantly (P≤0.05) from those of Nile tilapia received Blue tilapia DNA. The results of RAPD fingerprinting showed highly genetic polymorphic percentage among purebreds, genetically modified fish. The results of the present work suggest that, genetically modified Oreochromis niloticus with extraordinary growth rate can be produced by transferring a foreign DNA isolated from O. aureus or Cyprinus carpio as a feasible and fast methodology. So the present investigation recommended the usage of DNA transfer as a satisfactory technique for fish improvement to modify the fish species to be more efficient in terms of growth performance and feed utilization.
Biography Samy Yehya El-Zaeem is a Professor at Animal and Fish Production Department, Faculty of Agriculture, Alexandria University, Egypt.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 39 Joseph Carlo V Vergel et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Functional elucidation of PvRaf from Penaeus vannamei using RNA interference Joseph Carlo V Vergel and Mary Beth B Maningas University of Santo Tomas, Philippines
hrimp aquaculture is a major source of income in the intertropical countries of the Southeast Asian region. However, a great Sdecline in production was observed due to a major viral pathogen, the White Spot Syndrome Virus (WSSV) which continues to prevail despite many preventive measures applied to deter the virus. RNA interference (RNAi) technology has been employed to reveal functions of sequence specific genes in the virus and its host with the aim of controlling WSSV by elucidating complex host-virus interactions. RNAi is a relatively new technology that introduces double stranded RNA to specifically destroy cognate mRNA. This study reports the first record of Raf gene in a crustacean system. PvRaf serves as central intermediate in many signalling pathways, ultimately regulating cell proliferation, differentiation, development, and innate immune system responses, by connecting upstream tyrosine kinases with downstream serine/threonine kinases. Moreover, PvRaf is ubiquitously expressed in the vital organs of the shrimp suggesting that it is essential to metabolic functions of the shrimp and may also play a role in its innate immune system as highlighted in its expression in the haemocytes. Four set-ups were prepared to analyze the sequence specific silencing of PvRaf namely: PvRaf-dsRNA treatment, GFP-dsRNA treatment, PBS treatment, and naive control. Statistical analysis of the mortality assay indicates that PvRaf-dsRNA treatment has a significant protective effect against WSSV compared to GFP-dsRNA and PBS treatments.
Biography Joseph Carlo V Vergel has completed his Bachelor of Science in Biology from the University of Santo Tomas-College of Science, Manila, Philippines. He is pursuing his Master of Science in Biological Sciences with major in Molecular Biology at the UST Graduate School as a research scholar under the Department of Science and Technology-Accelerated S&T Human Resource Development Program. In 2012, he was awarded Bayer Young Environmental Envoy by Bayer and United Nations Environment Programme. In 2014, his research study was among the national awardees of the BPI-DOST Science Awards. In 2015, he bagged the Best Oral Presentation at the 9th Symposium on Diseases in Asian Aquaculture in Ho Chi Minh City, Vietnam and received the Student Travel Award. In 2016, he was awarded a scholarship grant by the Japan Association of Student Services Organization at Yokohama National University, Japan.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 40 conferenceseries.com 918th Conference
5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Workshop Day 2
Aqua Europe 2017
Page 43 Zuoxi Ruan, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Zuoxi Ruan Shantou University, China
The influence of the N source on C allocation in the cyanobacterium Synechococcus sp. depends on energy availability
he assimilation of N-NO3- by algae requires more energy than that of N-NH4+. Therefore, the availability of either N-source Tmay have appreciable consequences for the energetic budget of cells. This becomes relevant especially when energy (i.e. light) is in short supply. Consequently, the impact of N assimilation on the overall energy budget of phytoplankton may vary appreciably depending on the season, the time of the day and the position of cells in the water column. Furthermore, C:N stoichiometry has physiological and structural constraints and a change in the C:N ratio is reflected by the cell organic composition, with obvious consequences for trophic webs. In this work, we used the cyanobacterium Synechococcus sp. UTEX 2380 to investigate if and to what extent N availability and chemical form influenced growth, elemental stoichiometry and
carbon allocation, under N or light limitation, in the presence of either NO3- or NH4+ as the N-source. When energy was
limiting, Synechococcus grew faster in NH4+ than in NO3-, and had higher C (20%), N (38%), S (30%) cell quotas (a similar trend was also found for Fe, Zn, and Cr). Also, more C was allocated to protein, with a decrease of the carbohydrate/protein ratio, whereas the lipid/protein ratio did not change. Energy limitation also led to higher (129%) biomass productivity. We interpreted these results as an indication that, under energy limitation, the use of the least expensive N source allows a greater investment into growth and altered cell organic composition.
Biography Zuoxi Ruan has great passion in research of Algal Physiology and Ecology. Two main lines of research have characterized his scientific activity: 1) Patterns of energy and C allocation in microalgae, which was done by investigating C and N acquisition and assimilation, and by studying different patterns of C allocation in macromolecular pools,
allow to link algal cell biology and ecology with respect to the interaction between nutrient use and C allocation; 2) CO2 concentrating mechanisms and carbon fixation in calcifying and non-calcifying phytoplankton and their interaction with ongoing global changes (ocean acidification and UV) were carried out by investigation of C acquisition, calcification and photosynthesis, as may provide an insight into the change of organic and inorganic pump in the future.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 44 Zheng Huaiping, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Zheng Huaiping Shantou University, China
Study on genes involved in biosynthesis of PUFA in marine bivalves UFA play an important role in development, reproduction, growth, etc., of marine bivalves. Previous research showed Pthat bivalve is rich in PUFA, especially EPA(C20:5n-3) and DHA(C22:6n-3), however, bivalve could biosynthesize PUFA is always a controversial topic. Radionuclide tracer analysis gave evidence some bivalve could de novo synthesis PUFA from precursors, which, however, could not exclude effect of microorganism inside the experimental animals. Four genes (ELOVL2- like, ELOVL4, FAD5 and FAD8) involved in PUFA metabolism were cloned in noble scallop Chlamys nobilis. ELOVL2-like and ELOVL4 could elongate C18 and C20 to C22 and C24 respectively, while FAD5 and FAD8 showed Δ5 and Δ8 desaturation activity respectively. Thesis studies firstly gave molecular evidence that scallop could at least biosynthesize AA and EPA through “Δ8 pathway”. DHA might not synthesize in noble scallop because no desaturase with Δ4 and Δ6 activity was found. It seemed impossible that the scallop biosynthesize DHA through the “Δ8 pathway” C24:5n-3→elongation→C26:5n- 3→Δ8desaturation→C26:6n-3→β-oxidation→C24:6n-3→β-oxidation→C22:6n-3(DHA), because ELOVL4 cloud not provide C26 substrates by elongating C24. As a result, noble scallop possibly relied heavily on diet DHA to optimize its health. Interestingly, ELOVL2-like, ELOVL4 and FAD8 genes showed less efficient activity towards−3 n PUFA substrates than their homologous n−6 substrates, resulting in a relative low efficiency to biosynthesis n−3 PUFA, implying an adaption to marine environment.
Biography Zheng Huaiping has his expertise in the Genetics and Breeding in the scallops. He completed his PhD in 2015 and Post-doctoral studies in 2017 both from Institute of Oceanology, Chinese Academy of Sciences, China. Since 2008, as a Director, he has worked at Marine Biology Institute, Shantou University. A new variety named “Nan’ao Golden Scallop” has been bred by his team using artificial selection. Moreover, he has focused on biosynthesis of PUFAs in marine bivalves in recent years. He has pub- lished more than 20 papers in reputed journals and has been serving as a reviewer for many reputed journals.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 45 conferenceseries.com 918th Conference
5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Scientific Tracks & Abstracts Day 2
Aqua Europe 2017
Page 47 Fabrice Teletchea et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
A critical reappraisal of fisheries in Saint-Pierre et Miquelon with emphasis on the economic, social and political consequences of the Northern cod collapse in the late 1980s. Fabrice Teletchea1 and Daniel Pauly2 1University of Lorraine, France 2University of British Columbia, Canada
ince 1816, the islands of Saint-Pierre et Miquelon, off Newfoundland have been the last foothold of France in North America. As Sin the province of Newfoundland and Labrador (Canada), the fishery of the Atlantic cod (Gadus morhua) was the raison d’être of this small archipelago. Yet, in 1992, the Canadian government decided to close the commercial cod fishery due to the collapse of the stocks. Based on the FAO database of fisheries landings, reconstructed catches from the Sea Around Us (www.seaaroundus.org) and grey literature, this study reappraises the history of the fisheries of these islands since 1950, focusing particularly on the consequences of the moratorium on cod fishing. This study revealed that the fishery fleet based in the Saint-Pierre et Miquelon archipelago changed dramatically, from small traditional boats (‘dories’) to large trawlers reaching up to 50 m. Catches strongly increased in the 1970s and 1980s, reaching 23,800 tonnes in 1991. At its peak, nearly half of the private sector of Saint-Pierre et Miquelon was employed in the fisheries, including 140 fishermen and about 300 land-based persons, mainly in fish processing. In 1993, catches declined to less than 2% and remained very low for four consecutive years. Then, they increased again up to 6,690 tons in 2000 before decreasing ever since. In the past two decades, the number of people involved in the fishery sector has dropped to a few dozens. Today, nearly half of the active population is government employees, which has allowed it to remain relatively stable (at about 6,300 inhabitants). This contrasts to nearby Canadian towns (Fortune, Grand Bank) where more than 20% of the population has left.
Biography Fabrice Teletchea is working on Fisheries from the past 15 years. He had first worked on Fish Taxonomy and then moved to the study of Fish Domestication in Aquaculture. He also developed a comparative framework of the reproductive strategies of European freshwater fish species in order to better understand the different trade-offs observed at the early life stages of fish, to help domesticating them more efficiently.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 49 Anjanayappa H N et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Breeding biology of Priacanthus hamrur (Forsskal) of Mangalore coast, Karnataka, India Anjanayappa H N, Benakappa S, Ramachandra Naik A T, Nayana P and Rajesh D P College of Fisheries, India
ishes of the family Priacanthidae popularly called big eye or bulls eye. Priacanthus hamrur is an important deep-water inhabitant Fof great commercial value. High percentage of landings of Priacanthidae used as raw material for Surimi, sausage and other fishery by-products. Presently, it has great demand in Singapore, Thailand, Taiwan, Hong Kong and other countries. For the maturation studies, samples were collected from commercial landing centre, Mangalore. Studies on reproductive biology showed that Priacanthus hamrur spawns twice in a year; the spawning season extends from March to May and October to November. Based on the percentage occurrence of mature fishes in various size group it was inferred that male attained maturity at smaller size than female. This study will enable us to understand the spawning periodicity, cyclic morphological changes in male, female gonads and also it helps to improve stock size by enforcing fishing ban in particular season by assessing spawning periodicity.
Biography Anjanayappa H N is working as Professor and Head of Department of Fisheries Resources and Management, College of Fisheries, Mangalore, Karnataka, India.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 50 Kiuomars Rohani-Ghadikolaei et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Evaluation of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) culturing management in Teyab farms, Hormozgan, Iran Kiuomars Rohani-Ghadikolaei, Eesa Abdolalian, Hojatollah Fourooghifard, Maryam Moezzi and Mohammad Seddiq Mortazavi Persian Gulf and Oman Sea Ecological Research Institute, Iran
ue to outbreak of white spot disease (WSD), in 2004, that caused mass mortality of the indigenous species Penaeus indicus in Dsouthern shrimp farms of Iran, Litopenaeus vannamei has introduced to Iranian shrimp culture industry, and now is farmed in southern and northern coastal parts of Iran on a commercial scale. Considering shrimp farming is as a new and improving aquaculture practice in Iran, it faces some challenging problems in terms of culturing management that need to be monitored. The present study purposed to assess the performance of shrimp farming management in 3 randomly selected farms in Teyab, Hormozgan, Iran. The results showed that, although the greatest diversity in the Teyab farms were related to diatoms, but cyanophytes showed higher density than the diatoms. The results indicated that during the culturing period, mean values for temperature, salinity, pH and dissolved oxygen (DO) were not significantly different in farms, whereas the levels for Chlorophyll-a (Chl-a) and total organic matter (TOM) showed the highest content onwards to the end of culturing period. The average total length or final weight showed no significant differences in 3 farms at the end of culturing period. Positive correlation was found between pH and water temperature, pH and DO, pH and ammonia; Chl-a and DO; growth parameters and DO, growth parameters and Chl-a. However a negative correlation was observed between TOM and pH, TOM and water temperature, TOM and ammonia, TOM and pH; Chl-a and water temperature, Chl-a and salinity, Chl-a and transparency; growth parameters and salinity, growth parameters and pH, growth parameters and ammonia. However, water quality parameters should be monitored to serve as guide for managing a pond so that conditions that can adversely affect the growth of shrimp can be avoided.
Biography Kiuomars Rohani-Ghadikolaei has completed his PhD from University Science Malaysia (USM), Malaysia. He is the Head of Aquaculture Department of Persian Gulf and Oman Sea Ecological Research Institute affiliated to Iranian Fisheries Research Sciences Organization. He has published more than 4 papers in reputed journals and presented more than 6 papers in international conferences; as well as has been serving as an Editorial Board Member of Iranian fisheries research journals.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 51 Shokri Omar Mustafa, J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
The impact of climate change on species and number of fish on Tigris river in Mosul Dam/Duhok, Kurdistan Region of Iraq Shokri Omar Mustafa Ministry of Agriculture and Water Resources- Kurdistan Regional Government, Iraq
his study is the only research that has been conducted on Tigris river in Mosul Damin Sumel District (Duhok Governorate, TKurdistan Region of Iraq. As this research is the only research that has been done on Tigris river, it needs more studies to be done on it because the climate change has already had its impact on it. A new species of fish which is locally called as Shikhat fish that impact negatively on balance of fish species, especially carp fish and Shabout fish in the selected part of the dam of the Tigris river that is located in Duhok Governorate for the safe, fresh and clean water. This part is considered as the best part for raising fish with cage fish culture because we used many square and circle cage fish cultures on it and also, we used Fish hatchery ponds (Indoor), all these reasons helped us to succeed our study in terms of environment, health and economic aspects. The main aspect is to know what the causes are for negative effects on fish species’ balancing especially carp and Shabout fish in Tigris river in Mosul Dam. The fish survey was divided into two groups, one group includes carp and Shabout fish, and the other group includes Shikhat fish. Furthermore, water is supplied to Mosul Dam from Tigirs river at Turkey. At the beginning, we thought that Shikhat fish comes from Turkey through Tigris river, but the results showed an opposite impact which is due to the impact of the global warming. It can tolerate bad circumstances such as low dissolved oxygen, high temperature, low nutrient value of water and other nutrients such as phytoplankton and zooplankton. There is an increase in the number of Shikhat fish alarmingly since 2010 to date. This indicates that the Shikhat fish is spreading very quickly in Mosul Dam in Tigris river and this can impact negatively on other species of fish. This ultimately affects the fishermen in Duhok Province in terms of decrease in their income, which leads to socio-economic problems. In our study there were significant differences in weight between Shikhat and carp fish and Shabout fish, and it has an obvious explanation because of difference in species of fish. It may get curious result that needs further investigation. Perhaps this reflects species of fish, level and source of water, natural food variety such as plankton to name but a few of the variables that could be investigated. It also led us to follow up the research, recording more data and continuing research for the future.
Biography Shokri Omar Mustafa has his experience in Fish Resources sector in terms of fish nutrition, cage fish culture, Trout Salmon fish farm, poultry nutrition and biotechnology and animal production sector for more than 22 years. Currently, he is working as the Head of Fish Resources department and Nutritionist at the Ministry of Agriculture and Water Resources in Kurdistan Region of Iraq.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
Page 52 Istiaq Ahmad Chowdhury et al., J Aquac Res Development 2017, 8:2 (Suppl) conferenceseries.com http://dx.doi.org/10.4172/2155-9546.C1.014 5th Euro Global Summit on Aquaculture & Fisheries March 30-31, 2017 Madrid, Spain
Development of probiotics based culture system of Macrobrachium rosenbergii using different stocking densities Istiaq Ahmad Chowdhury and Nani Gopal Das University of Chittagong, Bangladesh
wing to the problem of antibiotic resistance and subsequent reluctance of using antibiotics, probiotics use in aquaculture Ois becoming popular day by day. One experimental design with 150 days culture period of Macrobrachium rosenbergii was conducted with 3 treatments maintaining stocking density of 02/m2, 03/m2 and 04/m2 in T1, T2 and T3 respectively. Each of the treatments was with 3 replicates where each replicate was segmented into two parts to separate probiotics and non-probiotics based culture system. The higher body weight of 63.7 g was recorded in lower SD of T1 in comparison to lower body weight of 55.7 g and 43.0 g in higher SD of T2 and T3 respectively for probiotics application segments. The average body weight of 55.7 g, 46.7 g and 37 g respectively were found for the same treatments in non-probiotics segments. The average survival rate of 69.3%, 62.7% and 58.3% were recorded in probiotics and 68.3%, 63% and 57.7% respectively in non-probiotics treatments. Average daily growth rate and gross production were found better in probiotics than that of non-probiotic segments in all the treatments. Average daily growth rate of T1 was found 0.41 g and 0.36 g respectively for probiotic and non-probiotic segments. Similarly for T2 and T3 average daily growth rate were found 0.35 g and 0.27 g for probiotics and 0.30 g, 0.23 g for non-probiotic segments respectively. Gross average production showed better result of 103 g/m2/crop in T2 probiotics treated segment than that of other two results of 87.23 g/m2 and 98.10 g/m2 in T1 and T3 treatments respectively whereas 74.62 g, 87.23 g and 84.26 g/m2/crop was recorded in T1, T2 and T3 respectively in non- probiotic treatments. Abiotic parameters in all segments of 3 treatments were within the optimum ranges for M. rosenbergii culture during the study period.
Biography Istiaq Ahmad Chowdhury is currently working at University of Chittagong, Bangladesh.
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J Aquac Res Development Volume 8, Issue 2 (Suppl) ISSN: 2155-9546 JARD, an open access journal Aqua Europe 2017 March 30-31, 2017
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