r:; PHOTOMICROGRAPHY WITH THE OM SYSTEM
"From the stars to microorganisms" is can be tackled by this superbly com owner of an OM camera to the exciting the theme of the Olympus OM System prehensive photographic system. This possibilities of photomicrography with and there is no limit to the subjects that booklet is intended to in traduce the the OM System Photomicro units. r;;J TABLE OF CONTENTS
e THE PHOTOMICROGRAPHIC e M ICROSCOPE CONNECTING e SPECIAL UNITS ...... 16 WORLD ...... 3 UNITS ...... 12 Photomicrographic Exposure e PHOTOMICROGRAPHY Eyepiece Adapters PM-ADF, Meter EMM-7 ...... 16 GROUP SYSTEM CHART...... 5 PM-ADP & PM-ADG ...... 12 Screen Viewer PM -VSC ...... 17 e CHART OF PHOTOGRAPHIC Photomicrographic Supporting 5X Magnifier ...... 17 RANGES ...... 6 Stand PM-PSS ...... 12 Focusing Magnifier FT ...... 17 e SELECTION OF VIEWFINDER e FULL V AUTOMATIC PH OTO- e OTHER OM SYSTEM UN ITS ...... 19 UNITS ...... 7 MICROGRAPHIC SYSTEM ...... 13 Motor Drive Group ...... 19 Focusing Screens 1...... 7 Auto-Photomicrographic System Recordata Back 1 ...... 20 Varimagni Finder ...... 8 PM-10-A ...... 13 OM-Mount Endoscope Adapter...... 20 E yecu p 1 ...... 8 Automatic Exposure Body OM-Mount MTX Adapter ...... 20 Dioptric Correction Lenses 1 ...... 8 PM-PBA ...... 14 e HOW TO USE THE Eyecoupler ...... 8 Automatic Exposure Control Box PHOTOMICRO ADAPTERS L & H e SELECTION OF CONNECTING PM -CBA ...... 14 •••• 0 ••• 0 •••••••••••• 0 •• 0 0 • • • • • • • • • • • • • • • • • 21 UNITS ...... 9 Focusing Telescope PM-VS ...... 14 e USE OF THE ADAPTER L ...... 22 e CAMERA CONNECTING UNITS .. 11 e MANUAL PHOTOMICRO· Function of the Adapter L ...... 22 OM-Mount Photomicro Adapter L. . 11 GRAPHIC SYSTEM ...... 15 e USE OF PHOTOMICROGRAPHIC OM·Mount Photomicro Adapter H .. 11 Manual Photomicrographic System STAND PM-PSS ...... 26 35mm SLR Camera Adapter PM-1 0-M ...... 15 e USE OF THE ADAPTER H ...... 27 PM-D35S ...... 11 Manual Exposure Body PM-PBM ... 16 2 ~ THE PHOTOMICROGRAPHIC WORLD
The next time you come across a pretty flower, such as a morning glory, during a stroll, try collecting some of the pollen and preserving it in an alcohol solution in a watch glass. Then take a look at the pollen through a microscope with a 10x eyepiece and 10x objective. The wh ite pollen will swell to the size of golf balls. To get the best image of this kind of subject, a highly suitable microscope illuminator is the Olympus Trans·llluminator Model LSD, whose light intensity can be adjusted by the voltage control of the transformer pro vided. However, since changes in voltage affect the color temperature of the light source, for color photography it is preferable to adjust the intensity by means of neutral density compensation (NO) filters. An interesting exercise is to collect the pollen of various different flowers in separate watch glasses, and stain them with easily available pigments. These w ill not only provide microscopic images of great beauty, but w ill allow you to study the form, texture and composition of the pollen samples. Place a circle of black paper some 10- 15mm in diameter on a circular ground glass of 32mm d iameter, and insert this "bulls eye" glass into the filter mount of the substage condenser. Open the aperture iris diaphragm of the condenser fully, adjusting the concave m irror in the microscope base so that proper illumination is directed to the specimen, and you will be rewarded by a brilliant image of beautifully stained pollen samples against a dark back still more spectacular. Photo 2 (front the less the resolving power. You should ground_ You might well be tempted to cover, left) of a spider's web utilizes make full use of these optical properties. capture th is vibrant, living image for this effect. If you have an Abbe condenser, un permanent record. If so, you have only Using a polarizing filter is another way screwing the top lens of the condenser to mount the OM camera body and an to ensure beautiful coloration for for use with the objective 4x will give eyepiece of your choice onto t he micro photomicrography. Rotate the polarizer more even illumination. scope by means of the Photomicro on the microscope filter mount to get Make sure that the light beam emitted Adapter L and an Eyepiece Adapter. If the darkest possible f ield of view as by the illuminator or reflected by the your microscope is a drawtube type, seen through the eyepiece. A crystal substage m irror passes through the make sure to tighten the coarse adjust specimen placed in this dark field w ill objective and goes toward the eyepiece ment knobs before mounting the camera, ta ke on the most beautiful hues. (See on a correct path. Usually the plain so as to avoid the risk of the drawtube Photo 3 of Glucuronic acid on the surface of the mirror is used w ith bright dropping suddenly under the extra front cover, right.) If you place a thin field light. When the Trans-Illuminator weight. To focus the camera accurately mica filter in the condenser, the LSD (a low voltage illuminator with a you should use the interchangeable beautiful color is spread over the field. 6V, 5A bulb) is used, the light beam can Focusing Screen 1-12. To take the (See Photo 4 of a Quinine crystal on be adjusted by means of the field iris picture, if you are using an OM-2 camera the front cover, center.) diaphragm, bulb centration and focusing on "AUTO", simply press the shutter Other equipment needed for successful adjustment of the illuminator. A button. For " MANUAL" operation with photomicrography includes a micro practical way to check for correct the OM-1 or OM-2, you must first scope. A medium class model •s illumination is to put a piece of white rotate the shutter d ial to centralize the satisfactory for this purpose. Objectives paper in place of the specimen on the indicator needle on the exposure index of 4x and 1 Ox magnifications are most microscope stage. A simple way to in the viewfinder. If necessary, f ine frequently used. A 40x objective is the ensure appropriate, effect ive illumina adjustments can be made by adjusting next higher power. As the magnification tion is to align the microscope and the aperture iris diaphragm of the increases, so does the resolution, but illuminator base on a sheet of paper, condenser. It is also necessary to put the f ield of view and depth of field marking in their relative positions for color temperature compensation filte rs become smaller. For eyepieces, the photo easy realignment during subsequent use. in front of the m icroscope illuminator. eyepieces F K, which are specially If the m icroscope has a built-in Photo 1 (P.30) shows morning glory designed for photomicrography, are illuminator this procedure is still simpler. pollen photographed in this w ay. When especially recommended. The eyepieces (Y. I to, photographer ) the pollen is dispersed in the bright P5x and P10x are also highly suitable. field of view, advance the exposure one As with the objectives, higher magnif ica- stop more than indicated by the meter. t ion eyepieces decrease the field of By sticking four sector shaped pieces view and the image brightness. Excessive of variously colored cellophane, e.g. objective and eyepiece magnifications red, green, yellow and b lue, around the shou Id therefore be avoided. periphery of the bulls eye on the 32mm The smaller the aperture of the condenser diameter glass, you can obtain a vari iris diaphragm, the darker the field of colored background to make the image view, the large--r the-4 ----depth------of f ield, and ------~ PHOTOMICROGRAPHY GROUP SYSTEM CHART
OBJECTIVE LENS MOU'JT PM- Mlob DIOPTRIC OOlREC TIC)N LENS l EYECUP I ~ d FOCUSING MAGNIFIEA fT f OCUSING TELESCOPE PM VS -0 ~ ~~:!, ::. :..w ~."' 'f ~ - it=IP - TUB£ PM $01.1 ~ ~ -1 SCREEN VIEWER '-=-' PM.VSC AUTO BELLOWS "'"r> 5 X MAGNIFIER CAfJL£ RELEASE C:i~u~~ VARIMA
5 ~ CHART OF PHOTOGRAPHIC RANGES
06JECT (mm) 240X360 120X 180
MAGNIFICATION 1 10 I , 2 1 2 • oo 1 r ZUIKO I N TERCH~NGEABL E 1/7 1/10" flTEIICHANCEABU LENSES ~1fT lENS GROUP
> • ®MACRO 50mm Fl.5 ~ FOCUS# PHOTOM ICROGRAPHY 1 '1 •• GROUP •· ' 7 ' ' ' J 0 "'0 ...::t.... SIANOARO LENS MACROPHOTOGRAPHY :E • dO GROUP ,.. "' HANDY COPY STAND ...... >< ..:> ~SELECTION OF VIEWFINDER UNITS ------The single lens reflex camera viewfinder plays a vitally important role in photo· - \ micrography. The photographer looks through the finder to adjust focus, lighting and composition. In photomicro graphy, the picture magnification is extremely large compared with ordinary photography. The greater the image 8Y magnification, the more conspicuous the graininess of the focusing screen, and the more difficult it is to focus and ensure proper lighting. For this reason, th e Focusing Screen 1-1 3, usually mount ed on the OM body as standard, • Focusing Screens 1 should be replaced with the Macroprism· Of the 13 OM System in terchangeable matte Focusing Screen 1-12, specially focusing screens, the Cross Hairs-Clear designed for photomicrography. This Field type 1-12 is exclusively used in transparent screen with a cross hairs photomicrography. This transparent reticle in the center provides an un screen assures an unusually bright field usually bright clear viewing area. of view with high contrast. To focus, To focus with the Focusing Screen 1·12, first correct your visual acuity with correct your diopter to see the cross Dioptric Correction Lenses or the hairs and bring the subject into focus. Varimagni Finder in conjunction with When the sharp images of the cross hairs the Eyecoupler, so that the double cross and the subject are seen simultaneously, hairs can be seen clearly and separately. the subject is correctly focused. Then bring the subject into focus so Photomicrography requires more accu that the cross hairs and subject can be rate diopter correction than general seen clearly at the same time. The TTL photography. Make a point of using meter indicates proper exposure, but the most effective combination of depending on the subject conditions, the dioptric correction lenses and focusing reading shou ld be modified by adjust· screens. men t of shutter speed, apert ure, etc. ------7------• Varimagni Finder • Eyecup 1 • Eyecoupler The Varimagni Finder magnifies the Designed to prevent glare and loss of The E yecoupler 1s used with the OM viewfinder image, and rotates through contrast caused by stray light hitting the body and the Varimagni Finder for 360° allowing accurate focusing from eyepiece. Made of rubber for soft photomicrography. 1t attaches between any position, including waist-level and contact with the eye socket. A dioptric the camera and the Varimagni F inder, downward viewing camera positions. It correction lens can be fitted into a slot extending the Finder away from the si ides onto the viewfinder and can be in the Eyecup and is held by a threaded camera for more convenient viewing. adjusted for individual eyesight. retaining ring. The OM body requ ires When using the Motor Drive 1 in I m ages reflected on the finder glass can use of the Eyecup 1 or Varimagni Finder conjunction with the 250 F ilm Back 1, be changed from 1.2x to 2.5x for photomicrography. 'the Eyecoupler is required for mounting magnification by a convenient selector the Eyecup 1 to the camera. switch. To view the entire field through • Dioptric Correction Lenses 1 the eyepiece for framing and composing, If the Varimagni Finder is not available, set the switch to "1.2x". Then, for Dioptric Correction Lenses should be critical focusing, turn to "2.5x". used when necessary to assist focusing. For photomicrography, the Eyecoupler These lenses snap into a slot provided should be used between the camera and in the Eyecup. T hey are available in 8 the Varimagni Finder. different diopters: +2, + 1, 0 diopters for long sight; - 1, - 2, -3, - 4 and - 5 diopters for short sight. The appropriate choice of Dioptric Correction Lenses eliminates the need for eyeglasses. ------8------~ SELECTION OF CONNECTING UNITS For photomicrography, the camera must be mounted on a microscope by means of connecting devices. One of the key functions of these devices is to prevent the t ransmission of sh utter v ibration to the microscope. As the effec ts of this vibration depend on the magnifications involved, connecting units of the camera and microscope must be selected accord ing to your photographic purposes. The connecting units used on the camera include the Photomicro Adapter L for total magnifications up to 250x (ch iefly with objectives of up to 20x ), or the Ph otomicro Adapter H for magnifications higher than 250x. The Photomicrographic Supporting Stand PM-PSS reduces any vibration transmitted with the use of these adapters. The connecting units used on the micro scope are the Eyepiece A dapters PM ADF, PM·A DP and PM·A DG, according to the type of eyepiece in use. A series of FK Eyepieces provides the most versatile means for high reso lution photomicro graphy, and the eyepieces P, corrected for chromatic aberration and astigmatism, are used for both observation and photo· micrograph y w ith high power objectives. Th e eyepieces G are for use in stereo ". t. • • m icroscopy and photomicrography . T he diagram at righ t illustrat es t wo ex OM Body amples of the most typical combinations Automatic Varimagni Finder Exposure of connecting units av ailable in the O M liP ~ -o Cable Release Control Box Sy stem. Y o ur selection should be based SA· 11 I PM-CBA I= on the use y ou i ntend to make of t hem . ~ @0 ~ OM-Mount (Typical Use of OM-Mount (Typical Use of OM-Mount Photomicro Adapter H) Photomicro Adapter L) IO---- ~ CAMERA CONNECTING UNITS ...=..==-=------=-==:::==:::::=:;- l! n;r:J • • OM-Mount Photomicro Adapter L • OM·Mount Photomicro Adapter H • 35mm SLR Camera Adapter PM·D35S For photomicrography with the OM For mounting the OM body on the This is used with the OM-Mount Photo body, the Adapter L is used to mount a Photomicrographic System PM-1 0, auto· micro Adapter H to connect the Auto· low power eyepiece on the camera. matic or manual version, or the matic or Manual Exposure Body, PM· For this, each eyepiece requires an eye· Macrophotographic Equipment PMT-35 PBA or PM·PBM, of the Model PM·1 0 piece adapter for use with Olympus in conjunction with high power objec· to the OM body for photomicrographic microscopes other than the Universal tives, the Adapter H is used together work. Research Microscope VAN OX and the with the 35mm SLR Camera Adapter Photomicrograph ic Stand PM·PSS. PM-D35S. The Adapter L accepts the Eyepiece T he Adapter H also permits attachment Adapter on its slip-on mount and the of the OM body to the Olympus OM body on its bayonet mount. Motorized Operating Microscope Model The Focusing Screen 1-12 facilitates OMB for recording of micro-surgery . focusing for photomicrography. The Focusing Screen 1· 12 is recommend· ed for use w ith the Adapter H in photomicrography. -----11 - r:; MICROSCOPE CONNECTING UNITS Photomicrographic Supporti ng Stand PM-PSS • Eyepiece Adapters PM-ADF. PM ADP & PM-ADG * These adapters are used between eye piece and microscope. They are available in three types: Adapter PM -ADF for photo eyepieces FK, Adapter PM-ADP Microscope for eyepieces P and Adapter PM-ADG for eyepieces G. Of these three eyepieces, the newly designed photo eyepieces • Photomicrographic Supporting Stand "FK" are exceptionally w ell su ited for PM-PSS * photomicrography with the OM body. This is a convenient unit for photomicro· vibration from the microscope. The PM graphy at high magnifications. The unit PSS stand may be used with any Olympus supports the entire weight of the camera, microscopes or other microscopes having isolating it from the microscope. The a vertical photo tube or a vertical draw weight and solidity of a separate stand tube. Camera and microscope operation alone, tends to reduce any transmitted is not impaired by the use of the stand, * N OTE: Contact the O lympu s M icro· vibration. Other bench vibrations are and all controls remain fully accessible. scope D istributors for further absorbed by the stand's rubber feet and The Photomicro Adapter L or H is details. a rubber mat m inimizes transmission of required for use with the OM body. 12 --- r;:;J FULLY AUTOMATIC PHOTOMICROGRAPHIC SYSTEM • Auto-Photomicrographic System PM - 10-A * The PM-10-A is one of the main micro scope connecting units. The built-in vibration-proof electronic shutter permits a wide range of automatic exposures from 1/ 100 sec. to 32 minutes, and is especially suitable for color photography. The PM-10-A consists of 17 units, includ ing the Automatic Exposure Body PM PBA, Control Box PM-CBA, etc., to automatically determine correct expo sure time, compensate for reciprocity failure, and advance film after each exposure. A built-in calor temperature metering and regulating system balances the calor temperature of the light source with the calor temperature of the film. Easy interchangeability of many acces sories permits use of various large format ' film sizes as w ell as 35mm sizes. T he PM-1O ·A requires the Photomicro Adapt· er H and 35mm S LR Camera Adapter PM-0355 to mount the OM-1 or OM-2. lt is necessary to set the OM-2 to manual operation for use with the PM-1O · A, since the PM-10-A functions as a com pletely automatic photomicrographic exposure system. • Automatic Exposure Body PM·PBA* • Automatic Exposure Control Box • Focusing Telescope PM-VS * PM·CBA * The PM·PBA Exposure Body automati· Used with either the Automatic or cally determines the correct exposure This is a control unit for the PM-PBA. Manual Exposure Body. Provided w ith a time in conjunction with the Automatic Its console cabinet is provided with double cross-line diopter adjustment, Exposure Control Box PM· CBA. It in· switches for automatic exposure, time four fixed frame reticles indicating corporates electronically controlled shut· exposure and flash synchronization, and 35mm, 120 roll film. 3Y." x 4Y." and ter in an automatic exposure range from dials for ASA fine ad justment and color 4" x 5" film sizes, and a knurled ring 32 min. to 1/ 100 sec., 3 position light temperature adjustment. Push-button to clamp to the Exposure Body. path selector knob with sliding prisms, operation permits automatic shutter re quick clamp adapter for attachment to lease, manual shutter operation, film a microscope photo tube, and connecting advance and light check. cord to Control Box PM -CB A. The PM -PBA requires the Photomicro Adapter H and 35mm SLR Adapter PM D35S to mount the OM body. * NOTE: Contact the O lympu s M icr o· scope Distrib u t ors for fu rther deta ils. ---!4------~ MANUAL PHOTOMICROGRAPHIC SYSTEM • Manual Photomicrographic System PM-10-M * This is a popular manual version of the PM-10, consisting of 8 units, that in cludes the Manual Exposure Body PM PBM, Eyepiece Adapters, Focusing Telescope, Magnifier, etc. The PM-10-M, when used with the OM-1 or OM-2 in manual operation, facilitates photomicrography especially at high magnifications. This system requires the Photomicro Adapter H and 35mm SLR Adapter PM-0355 for mounting on a microscope with the Photomicrographic Supporting Stand PM-PSS or Eyepiece Adapters. The light path selector control can be adjusted to transmit all light to the observation eyepiece for focusing and composing in low light situations, to d ivide light between viewing and f ilm planes, or to send all I ight to the I igh 1 measuring port, which accepts the photosensitive cell of the Photomicro graphic Meter Model EMM-7. The OM-2 does not require the EMM-7 because of its built-in automatic exposure control system. r:; SPECIAL UNITS • Manual Exposure Body PM·PBM * Provided with an integrated mechanical shutter in a vibration-proof rubber mount, permitting shutter speeds from 1/250 to 1 sec and "B", 3 position light path selector knob and quick clamp mount f or the Focusing Telescope. Shutter cocking lever, flash sy nchro sock· et and shutter speed setting lever are also • Photomicrographic Exposure Meter provided . A light measuring port accepts EMM-7 * (shown right) the photocell of the Olympus Exposure This exposure meter assures accurate Meter EMM-7. T he cable re lease h as a control of both exposure and color tem time exposure locking device. Among perature with the Manual Exposure Body the focusing units available, the Screen PM -PBM. Complete with exposure and V iewer PM-VSC is recommended for *NOTE : Contact the Olympus Micro· color temperature probes, color corn· objectives lower than 4x, the Focusing scope Distributors for f u rthe r pensating filters and adapter for use with Telescope PM-VS for higher objectives. details. the OM body. ---:6i------• Scr een Viewer PM-VSC * • 5X Magnifier * • Focusing Magnifier FT* Designed for use with objectives of up T his magnifier is used with the Screen The Magnifier FT enables the photo to 4x magnifications. A hood is provided Viewer to facilitate more critical focus grapher to magnify the central part of to reduce extraneous light on the view ing. The front lens of this unit can be the image and bring the subject into ing screen. A clamping ring holds the moved in and out to magnify any part sharp focus in conjunction with the Viewer to the automatic or manual of the subject area and focus correctly. Focusing Telescope. The front lens por exposure body. tion of the Focusing Magnifier can be displaced laterally to permit focusing on the frame reticles of the Focusing Tele scope. The mounting r ing fits on the front lens portion. *NOTE: Contact t h e Olympus Micro scope D istributors for further details. 17 P' . , ...... ). ~ I ~ ~ ' (. / "\ t ~ ~. ~ • • • •• ~lE~( ' ~ c .. f:! 0~ ~ .... ~- * r "1*., '(-. 1 0<+ Leaf of camellia 18 r:; OTHER OM SYSTEM UNITS -- • Motor Drive Group The Motor Drive Group includes a number of units for sequential or inter· mittent exposures in close-up and copy· ing photography, photomacrography and photomicrography. A remote control mechanism can be used for a series of exposures over a short time period, ex· posures taken intermittently in conjunc· tion with a timer for photography of ce ll division over a prolonged time, etc., or a long series of exposures with bulk film. The Motor Drive 1 is the core of the OM System Motor Drive Group. lt mounts directly on the ca mera base to make one of the most compact and maneuverable motor drive systems avail· able. The M. 18V Control Grip 1, re chargeable M. 15V N i·Cd Control Pack 1, or M.AC Adapter attaches to the Motor Drive 1 to power and control it. The Motor Drive 1 not only frees the photographer from the burden of advanc· ing the film manually. but also allows him to shoot series of pictures that might be lost through the time-consum ing manual w ind-on method. --]9------• Recordata Back 1 The Recordata Back 1 converts the OM body into a data camera, by enabling imprinting of numerical and alphabetical data directly onto the photograph. An indicator switch permits selection be· · tween color and B&W photography. Electrical connection is made by insert ing the connecting plug of the Back into • OM-Mount Endoscope Adapter * • OM-Mount MTX Adapter * the synchro socket of the OM body. * This adapter is used to mount the OM A bayonet mount adapter allowing the body on the Olympus fiberscopes for OM body to be easi ly attached to the Operation Microscope Model MTX to endoscopic photography. The Focusing meet the exacting requirements of micro Screen 1-9 is specially designed for this surgery. * NOTE: Con tact the O l ympus M icr o The OM System lets the Operation scope Distributors for further use. Microscope Model MTX record the opera details. tion in detail. When used with the **NOTE: Contact the 0 lympus F ibor· Motorized Operation Microscope Model scope Distributors for further OMB, the OM body requires the Photo micro Adapter H. details. ------~------. z: I' As previously mentioned, the connecting Whatever the magnification, to master units employed in the Photomicrography photomicrographic techniques you must Group can be d ivided into two groups, first become familiar with every unit. according to the total magnification. The following pages describe how to use One group cantering on the Photomicro the Photomicro Adapters l , H, Eyepiece Adapter l is chiefly used for total Adapters PM·AOF, PM·ADP, PM·ADG magnifications of 250x and lower; the and Photomicro Stand PM-PSS. other group, cantering on the Photo micro Adapter H, for magnifications higher than 250x. ------2£1------r;;J USE OF THE ADAPTER L Eyepiece Picture Magnification FK Eyepiece Power X -!() FK - Objective Power P or G Eyepiece Power X P&G Objective Power X Approx. 0.5 • Function of the Adapter l • Use the Adapter L. 3. Determine the magnification. Picture magnification is determined by For photomicrography with the OM 1. Replace the standard Focusing Screen the above formula. body, this adapter is used to mount a 1-13 with the Sc;een 1-12. photo eyepiece on the camera. The 2. Mount the Photomicro Adapter L to eyepieces available for this u se are the the OM body. The Adapter Lis attached FK, P and G. Each of them requires a to the camera in the same manner as a specific eyepiece adapter PM-ADF, PM normal interchangeable lens. ADP or PM-ADG. For 20X objectives, w ith total magnification up to 250x, the Adapter l directly connects to either of these three eyepiece adapters. Note, however, that the Olympus Universal Microscope VANOX and the Photo micrographic Stand PM-PSS do not require any eyepiece adapter for photo micrography with the OM body through the whole range of magnification. ------22---- AOOI:HOr L I= (! :J 1tn Screw j·3 p hOI(l , I ' E yop1Coe F K d I lr Sorc w I Pho1o Tubo 4. In photomicrography. microscopes 5. Select an eyepiece adapter suitable 6. Mount the Adapter L w ith camera require special eyepieces FK, P or G for for the eyepiece in use. ont o the m icroscope. use with the OM body and Adapter L • Eyepiece Adapter PM-ADF for Photo After selection of the Eyepiece/ Eyepiece for low power magnification. Eyepieces F K Adapter combination, mount the entire 1) When ei ther eyepiece F K or P is • Eyepiece Adapter PM -ADP for Eye camera assembly on the microscope as su itable, F K is preferable to P, since pieces P follows: it is specially designed for photo· • Eyepiece Adapter PM-ADG for Eye a. For Photo Eyepiece F K micrography. pieces G 1} Attach the Eyepiece Adapter PM 2) Only the objective has reso lving NOTE: ADF to the photo tube of m icro power. The eyepiece serves merely The VANOX and PM-PSS do not scope, and clamp with screw provided. to enlarge the image reso lved by the require any eyepiece adapter. 2) Insert the selected Eyepiece FK into objective. For a given total magnifica· the Eyepiece Adapter PM-ADF, fit tion involving d i fferent combinations the Adapter L over the Eyepiece of eyepieces and objectives, the use Adapter PM -ADF push ing all the of a higher power object ive will way down, and clamp the entire result in finer resolution. camera assembly. ------2~------Adapter L ACJaJ)tor 1.. Adaoler L ~~ I i~ Scrow I I 2 Eyep.e-oe J E 'lt"PietCCI' P Ad;:~~ptor G JSxor G 20x 11 .I PN·AOG I Eyepw.ece Adaptet" G IOX • PM-ADP IS Screw c:f I sorow I 3 PhOto Tube • ' PhOto Tll,bo I I b. For Eyepiece P c. For Eyepiece G10x d. For Eyepieces G 15x and G20x 1) Fit the Eyepiece Adapter PM-ADP 1 ) Unscrew the upper part of the Eye- 1) Attach the Eyepiece Adapter PM to the photo tube pushing all the piece Adapter PM-ADG, insert the ADG to the eyepiece sleeve, and way down. G 10x into the lower part, and replace clamp. 2) Insert the Eyepiece P into the photo the upper part of the Adapter. 2) Insert the G 15x or G20x into the tube. 2) Attach the Eyepiece/ Eyepiece Adapt· Adapter PM-ADG. 3) Pressing down the eyepiece against er combination to the Adapter L, and 3) Fit the Adapter L over the Eyepiece the photo tube slightly, raise the clamp. Adapter pushing all the way down, Eyepiece Adapter until it contacts 3) Insert th e entire camera assembly and clamp the en tire camera assembly. with the eyepiece flange (be sure the into the m icroscope eyepiece sleeve eyepiece flange is in contact with pushing all the way down, and clamp. both the photo tube and Eyepiece Adapter) and lock the adapter firmly with clamping screw. 4) Fit the Adapter L over the Eyepiece Adapter push ing all the way down and clamp the en tire camera assembly. ------24------7. Adjust the camera position. 8. Focus. 9. Determine the exposure. If it is necessary to adjust the camera 1) Focusing with the OM System Focus 1) For the OM-1 and OM-2 1n manual position, loosen the clamping screw pro ing Screen 1-1 2. operation: Rotate the shutter speed vided on the Adapter L, rotate the (1) Use the Dioptric Correction Lens ring or adjust light intensity by means camera to the desired position, and or the Varimagn i Finder, so that of voltage adjustment of the light reclamp. the double cross hairs within the source or NO filters, until the needle field of view can be clearly recogniz in the viewfinder comes to the center ed as two lines; otherwise correct of index marks. focus cannot be obtained. 2) For the OM·2 in automatic operation: (2) Adjust the coarse and fine adjust You can use the automatic exposure ment controls of the m icroscope to control system built in the OM-2. bring the specimen into focus. • If the subject is darker than the Check again if both the double background, turn the exposu re ring cross hairs and the specimen are or exposure compensation ring to equally sharp in focus. over-expose by one or two F stops according to the situation. On the contrary, if the subject is brighter than the background, turn the ring to under-expose. ------~------r;; USE OF PHOTOMICROGRAPHIC STAND PM-PSS -· • 3. Inser t the eyepiece into the photo 6. Lower the arm slowly until it stops tube. (at this height, the diaphragm inside the 1} For Ph oto Eyepiece FK Adapter L touches the top of the eye· Put the light shield tube on the piece). microscope photo tube, into· which Then raise the arm by about 1 mm so the selected Photo Eyepiece FK is that the Adapter L f loats off to the inserted slowly. Eyepiece. Take care not to allow the 2) For Eyepiece P per iphery of the Eyepiece or light shield Insert the selected Eyepiece P into tube to come into contact with the edge the photo tube without the light of the camera mounting hole. Then raise sh ield tube. the collar to support the arm. 7. Mount the OM body on the Adapter To set up the microscope on the PM 4. Looking through the camera mount PSS. as illustrated above. please read the ing hole in the arm, let down the arm L. instructions provided with the stand, and until the Eyepiece enters the hole (if 8. Focus and expose. then proceed as follows: necessary, adjust the microscope posi 1. Set the Focusing Screen 1-12 to the tion so as to accurately center the OM body. Eyepiece in the hole). 2. Place the m icroscope on the base of 5. Loosening the clamping screw provid the PM-PSS. so that the photo tube ed on the Adapter L, put the Adapter L comes directly under the camera support over the camera mounting hole, and mg arm. clamp. ------26------~ USE OF THE ADAPTER H The Adapter H is a convenient unit for • Use the Adapt er H. 3. Attach the 35mm SLR Camera high magnification photomicrography Adapter PM-035S to Automatic Ex· 1. Set the Focusing Screen 1-12 to the w ith the Automatic Exposure Body PM· posure Body PM-PBA or Manual Ex OM body. PBA or Manual Exposure Body PM-PBM. posure Body PM-PBM. 2. Mount the Photomicro Adapter H on The Adapter H is used with the PM· NOTE: the OM body in the same manner as a 0355. To install the PM-0355, align its nomal interchangeable lens. locating pin with the locating groove on the automatic or manual exposure body and tighten the clamping ring. Insert the Photomicro Adapter H plus OM body into the PM-0355, and clamp with locking knob. 27------Eyepiece Picture Magnification FK Power X FK Objective Power P or G Power X P&G Objective Power X Approx. 0.5 4. Determine the magnification. 7. Mount the Adapter H with camera 9. Focus and exposure. Picture magnification is determined by body onto the microscope. For easier and more accurate focusing, the above formula. 8. Adjust the camera position. it is recommended to use the PM·PBA 5. Olympus microscopes require Ev e· or PM·PBM. Their uses are explained in pieces F K, P or G for use with the OM detail in their instruction manuals. body and Adapter H for high power magnification. 6. Select an Eyepiece Adapter appropri· ate to the Eyepiece in use. ------~------r-- - Photo 2--- -t---Photo 4---+o----Photo 3 ----r Exposure Measurement 1---::-:-:--:--__:__ OM-1 OM-2 Automatic Usable with the TTL Usable either with the Exposure meter built into the automatic exposure meter Body OM-1, but use with the built into the OM-2 or PM-PBA control box PM -CBA with the PM-CBA. Photo 1 on the opposite page shows the pollen is preferable. --- of a morning glory. Manual Usable with the TTL Usable with the automatic (See page 4 for further details.) Exposure meter built into the exposure system built Body OM-1, but use with the into the OM-2. Front Cover Photos PM-PBM exposure meter EMM-7 Photo 2 : Spider's web and waterdrops. is preferable to obtain Photo 3 : Glucuronic acid. accurate measurement. Photo 4 : Quinine crystal. -----29---