Olympus OM System Manual for Photomicrography Group
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r:; PHOTOMICROGRAPHY WITH THE OM SYSTEM "From the stars to microorganisms" is can be tackled by this superbly com owner of an OM camera to the exciting the theme of the Olympus OM System prehensive photographic system. This possibilities of photomicrography with and there is no limit to the subjects that booklet is intended to in traduce the the OM System Photomicro units. r;;J TABLE OF CONTENTS e THE PHOTOMICROGRAPHIC e M ICROSCOPE CONNECTING e SPECIAL UNITS ...................... 16 WORLD ................................ 3 UNITS ........... ................ ....... 12 Photomicrographic Exposure e PHOTOMICROGRAPHY Eyepiece Adapters PM-ADF, Meter EMM-7 .......................... 16 GROUP SYSTEM CHART........... 5 PM-ADP & PM-ADG .................. 12 Screen Viewer PM -VSC ............... 17 e CHART OF PHOTOGRAPHIC Photomicrographic Supporting 5X Magnifier ......................... .. 17 RANGES . 6 Stand PM-PSS .. ........... ............ 12 Focusing Magnifier FT ............... 17 e SELECTION OF VIEWFINDER e FULL V AUTOMATIC PH OTO- e OTHER OM SYSTEM UN ITS ...... 19 UNITS .................................. 7 MICROGRAPHIC SYSTEM ......... 13 Motor Drive Group ................... 19 Focusing Screens 1.................... 7 Auto-Photomicrographic System Recordata Back 1 ..................... 20 Varimagni Finder .. ................... 8 PM-10-A .. ........ ... ....... .... .... .... 13 OM-Mount Endoscope Adapter... .. 20 E yecu p 1 . 8 Automatic Exposure Body OM-Mount MTX Adapter ............ 20 Dioptric Correction Lenses 1 . 8 PM-PBA .. .... ..... ... .... .... .. .. ... ... 14 e HOW TO USE THE Eyecoupler ................ ............ 8 Automatic Exposure Control Box PHOTOMICRO ADAPTERS L & H e SELECTION OF CONNECTING PM -CBA .......... .. .. .................. 14 •••• 0 ••• 0 •••••••••••• 0 •• 0 0 • • • • • • • • • • • • • • • • • 21 UNITS .................................. 9 Focusing Telescope PM-VS .......... 14 e USE OF THE ADAPTER L ......... 22 e CAMERA CONNECTING UNITS .. 11 e MANUAL PHOTOMICRO· Function of the Adapter L ..... .... .. 22 OM-Mount Photomicro Adapter L. 11 GRAPHIC SYSTEM ............ ...... 15 e USE OF PHOTOMICROGRAPHIC OM·Mount Photomicro Adapter H .. 11 Manual Photomicrographic System STAND PM-PSS .................. ..... 26 35mm SLR Camera Adapter PM-1 0-M ................................ 15 e USE OF THE ADAPTER H ......... 27 PM-D35S ............................... 11 Manual Exposure Body PM-PBM ... 16 2 ~ THE PHOTOMICROGRAPHIC WORLD The next time you come across a pretty flower, such as a morning glory, during a stroll, try collecting some of the pollen and preserving it in an alcohol solution in a watch glass. Then take a look at the pollen through a microscope with a 10x eyepiece and 10x objective. The wh ite pollen will swell to the size of golf balls. To get the best image of this kind of subject, a highly suitable microscope illuminator is the Olympus Trans·llluminator Model LSD, whose light intensity can be adjusted by the voltage control of the transformer pro vided. However, since changes in voltage affect the color temperature of the light source, for color photography it is preferable to adjust the intensity by means of neutral density compensation (NO) filters. An interesting exercise is to collect the pollen of various different flowers in separate watch glasses, and stain them with easily available pigments. These w ill not only provide microscopic images of great beauty, but w ill allow you to study the form, texture and composition of the pollen samples. Place a circle of black paper some 10- 15mm in diameter on a circular ground glass of 32mm d iameter, and insert this "bulls eye" glass into the filter mount of the substage condenser. Open the aperture iris diaphragm of the condenser fully, adjusting the concave m irror in the microscope base so that proper illumination is directed to the specimen, and you will be rewarded by a brilliant image of beautifully stained pollen samples against a dark back still more spectacular. Photo 2 (front the less the resolving power. You should ground_ You might well be tempted to cover, left) of a spider's web utilizes make full use of these optical properties. capture th is vibrant, living image for this effect. If you have an Abbe condenser, un permanent record. If so, you have only Using a polarizing filter is another way screwing the top lens of the condenser to mount the OM camera body and an to ensure beautiful coloration for for use with the objective 4x will give eyepiece of your choice onto t he micro photomicrography. Rotate the polarizer more even illumination. scope by means of the Photomicro on the microscope filter mount to get Make sure that the light beam emitted Adapter L and an Eyepiece Adapter. If the darkest possible f ield of view as by the illuminator or reflected by the your microscope is a drawtube type, seen through the eyepiece. A crystal substage m irror passes through the make sure to tighten the coarse adjust specimen placed in this dark field w ill objective and goes toward the eyepiece ment knobs before mounting the camera, ta ke on the most beautiful hues. (See on a correct path. Usually the plain so as to avoid the risk of the drawtube Photo 3 of Glucuronic acid on the surface of the mirror is used w ith bright dropping suddenly under the extra front cover, right.) If you place a thin field light. When the Trans-Illuminator weight. To focus the camera accurately mica filter in the condenser, the LSD (a low voltage illuminator with a you should use the interchangeable beautiful color is spread over the field. 6V, 5A bulb) is used, the light beam can Focusing Screen 1-12. To take the (See Photo 4 of a Quinine crystal on be adjusted by means of the field iris picture, if you are using an OM-2 camera the front cover, center.) diaphragm, bulb centration and focusing on "AUTO", simply press the shutter Other equipment needed for successful adjustment of the illuminator. A button. For " MANUAL" operation with photomicrography includes a micro practical way to check for correct the OM-1 or OM-2, you must first scope. A medium class model •s illumination is to put a piece of white rotate the shutter d ial to centralize the satisfactory for this purpose. Objectives paper in place of the specimen on the indicator needle on the exposure index of 4x and 1 Ox magnifications are most microscope stage. A simple way to in the viewfinder. If necessary, f ine frequently used. A 40x objective is the ensure appropriate, effect ive illumina adjustments can be made by adjusting next higher power. As the magnification tion is to align the microscope and the aperture iris diaphragm of the increases, so does the resolution, but illuminator base on a sheet of paper, condenser. It is also necessary to put the f ield of view and depth of field marking in their relative positions for color temperature compensation filte rs become smaller. For eyepieces, the photo easy realignment during subsequent use. in front of the m icroscope illuminator. eyepieces F K, which are specially If the m icroscope has a built-in Photo 1 (P.30) shows morning glory designed for photomicrography, are illuminator this procedure is still simpler. pollen photographed in this w ay. When especially recommended. The eyepieces (Y. I to, photographer ) the pollen is dispersed in the bright P5x and P10x are also highly suitable. field of view, advance the exposure one As with the objectives, higher magnif ica- stop more than indicated by the meter. t ion eyepieces decrease the field of By sticking four sector shaped pieces view and the image brightness. Excessive of variously colored cellophane, e.g. objective and eyepiece magnifications red, green, yellow and b lue, around the shou Id therefore be avoided. periphery of the bulls eye on the 32mm The smaller the aperture of the condenser diameter glass, you can obtain a vari iris diaphragm, the darker the field of colored background to make the image view, the large--r the-4 ----depth-------------------- of f ield, and ------------------ ~ PHOTOMICROGRAPHY GROUP SYSTEM CHART OBJECTIVE LENS MOU'JT PM- Mlob DIOPTRIC OOlREC TIC)N LENS l EYECUP I ~ d FOCUSING MAGNIFIEA fT f OCUSING TELESCOPE PM VS -0 ~ ~~:!, ::. :..w ~."' 'f ~ - it=IP - TUB£ PM $01.1 ~ ~ -1 SCREEN VIEWER '-=-' PM.VSC AUTO BELLOWS "'"r> 5 X MAGNIFIER CAfJL£ RELEASE C:i~u~~ VARIMA<J'JI FINDER MANUAL EXPOSURE r~ ') SR· Il OCOV PM PUM ,_ ~ JJ<; er- I 1- ]- ~ COPY STAND -~ @ EYEPIECES G fOCUSING SCREEN 1- 12 =I rD !, .......... i ) <'· <»A·MC:Uf'H I t Pti()TCMICRO 35mm SLR CAMERA '-D ADAPTER H ADAPTER PM"03!'>S AUTOMATIC EXPOSl,JRE EYEPIECE ~ BOOY PM.PSA ADAP TER r- PMADG l2],'~ L, ALj~ EYFPIECES L. (_ ---- !I ~ p r D - I .! J = .- .1 1-t- D _r- i )] ~}"""-,~ @ t- ~ !I PM AD '~_] ''_;J: RECOROATA BACK I I EYEPIECt S FK PHOTCf.oliCRoc.RAPHIC I~ oil ,_ SUPPCflTING STANOPM·PSS - ...._ 1- ~ 1- 00@@ ~ ~ _._ ~ 0 @@@@ E Y~CE r.= ADAPTER ~- ~ ~ EMM-7 I I I '! I@ PM ADF AUTOMATit,. [XP(f..URE -c. ~ CONTRCL BOX PM CllA c~ MOTOR DRIVE GROJP ~ OM-MOUNT PHOTOMICRO ADAPTER L 5 ~ CHART OF PHOTOGRAPHIC RANGES 06JECT (mm) 240X360 120X 180 <I$X72 24 X36 12XI8 4.8X7.2 2.4X3.6 1.2 ><1.8 AREA {tnth) (9UX14U )(4Jjx7,j',)(ill>.21U (:Hxlil)(Ux;ll} ( 1*.;X /:~)(J11 X /1. ) (/. x lt) GROUPS MAGNIFICATION 1 10 I , 2 1 2 • oo 1 r ZUIKO I N TERCH~NGEABL E 1/7 1/10" flTEIICHANCEABU LENSES ~1fT lENS GROUP > • ®MACRO 50mm Fl.5 ~ FOCUS#<C S t A('£ roc::us«: AM. roPY STAN> 0 "'0 ::t .... J8·-- .. f------r---. .__•_· _l])__ t_ : t_•_<AO<O--. 80mm H I :E I .ACRO 38mm f3.5 u I 8' •. 0• 0 .. ;;; ~ CO PHOTOM ICROGRAPHY 1 '1 •• GROUP •· ' 7 ' ' ' J 0 "'0 ...::t.... SIANOARO LENS MACROPHOTOGRAPHY :E • dO GROUP ,.. "' HANDY COPY STAND ...... >< ..:> ~SELECTION OF VIEWFINDER UNITS --------------------- The single lens reflex camera viewfinder plays a vitally important role in photo· - \ micrography.