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Characteristics of Size-Fractionized Phytoplankton and Their Response to Environmental Factors in Typical Lakes of Southeastern Hubei Province, China - 5891

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Characteristics of Size-Fractionized Phytoplankton and Their Response to Environmental Factors in Typical Lakes of Southeastern Hubei Province, China - 5891 Xie et al.: Characteristics of size-fractionized phytoplankton and their response to environmental factors in typical lakes of southeastern Hubei Province, China - 5891 - CHARACTERISTICS OF SIZE-FRACTIONIZED PHYTOPLANKTON AND THEIR RESPONSE TO ENVIRONMENTAL FACTORS IN TYPICAL LAKES OF SOUTHEASTERN HUBEI PROVINCE, CHINA XIE, Y.-Z.# – LIU, M.-Q.# – LIU, Z.-X. – HOU, J.-J.* – LIU, X.-X. – ZHOU, F. – CHEN, Q. – SUN, L.-L. Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi 435002, China Hubei Engineering Research Center of Typical Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi 435002, China National Demonstration Center for Experimental Biology Education, Hubei Normal University, Huangshi 435002, China #The two first authors made equal contributions to this paper *Corresponding author e-mail: [email protected]; phone: +86-71-4651-1613 (Received 15th Mar 2020; accepted 9th Jul 2020) Abstract. We studied the size-fractionized phytoplankton community structure characteristics and their response to environmental factors by investigating the photosynthetic pigment concentration and composition; composition and abundance of algal phyla levels; and environmental factors in typical lakes of Hubei Southeastern, China for four seasons. High-performance liquid chromatography (HLPC) results show that the total chlorophyll concentration was 36,418.62 mg/m3, and the contribution rates of microplankton, nanoplankton, and picoplankton to the total biomass were 13.43%, 49.08%, and 37.49%, respectively. Fucoxanthin, alloxanthin, zeaxanthin, and chlorophyll b were the main photosynthetic pigments. However, the spatial and temporal distribution had significant differences in the four typical lakes. Chemical taxonomy (CHEMTAX) calculation indicates that the dominant species were Diatoms and Cryptophytes in spring, Euglenophytes in summer, Euglenophytes and Cyanobacteria in autumn, and Euglenophytes and Cryptophytes in winter. The Chrysophyte and Dinoflagellates have the lowest proportion for all seasons. The redundancy analysis (RDA) demonstrates that the key environmental factors for the succession were Total Nitrogen (TN) and Total Phosphorus (TP). The application of the HPLC-CHEMTAX method has provided the first analysis of the community structure of size-fractionized phytoplankton in typical lakes of Hubei Southeast, China, and environmental factors affect the succession of size-fractionized phytoplankton over time. This study provides theoretical bases that the comprehensive research on different size phytoplankton in freshwater. Keywords: HPLC, community structure, photosynthetic pigment, chemical taxonomy, redundancy analysis Introduction East Lake (Wuchang District, Wuhan City, Hubei Province, China) is a typical semi- enclosed lake in the middle and lower reaches of the Yangtze River (Yun et al., 2015), and it is the second largest urban lake in China. The catchment area is 190 km2, and the lake water area is 34.59 km2. The average water depth is 2.2 m, with the highest value reaching 6 m. However, Wuhan is a typical industrial city, the domestic, industrial, and agricultural waste water discharge has significantly increased with its rapid industrialization and urbanization, which has resulted in the deterioration of the water APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 18(4):5891-5905. http://www.aloki.hu ● ISSN 1589 1623 (Print) ● ISSN 1785 0037 (Online) DOI: http://dx.doi.org/10.15666/aeer/1804_58915905 © 2020, ALÖKI Kft., Budapest, Hungary Xie et al.: Characteristics of size-fractionized phytoplankton and their response to environmental factors in typical lakes of southeastern Hubei Province, China - 5892 - quality of the East Lake. The trend has changed from phosphorus limitation to nitrogen restriction, and eutrophication is intensifying. Cihu Lake (Huangshi City, Hubei Province, China) is the largest lake in Huangshi with a water area of approximately 1.0 × 107 m2, catchment area of 6.28 × 107 m2, and average water depth of 1.75 m (Yan, et al., 2015). The effect of its geographical location (i.e., proximity to a city, low water level, and limited purification capacity) and absence of a reasonable treatment have resulted in a large amount of industrial wastewater discharge. Water pollution is serious concerning the lake’s eutrophic state. Qingshan Lake (Huangshigang District, Huangshi City, Hubei Province, China), which is located in the northern part of the Cihu Lake, typical urban lake, has a catchment area of 6.2 km2, water area of 0. 52 km2, and depth of 16.83 m (Li et al., 2013). It consists of four sub-lakes. Qinggang Lake (Huangshigang District, Huangshi City, Hubei Province, China) is located on the west bank of the Yangtze River and east of Qingshan Lake. Compared with Qingshan Lake, Qinggang Lake, Cihu and East Lake have more domestic sewage discharge, their smaller water areas, poorer self-recovery ability, and serious water pollution are often observed to be in a nutritious state. Phytoplankton participates in the material cycle and energy flow as the main contributors of primary productivity; they also play an important role in the freshwater ecological system (Callieri, 2008). Phytoplankton consists of microplankton (20- 200 µm), nanophytoplankton (2-20 µm), picophytoplankton (0.2-2 μm), and ultraphytoplankton (< 5 μm) (Robineau et al., 1999); each type has different contributions to the primary productivity and biomass. Nishibe et al. (2015) determined that the primary production was low in winter and composed mostly of small phytoplankton (< 10 μm), whereas large phytoplankton (> 10 μm) became the major producers in spring with high production. Current studies on the phytoplankton size structure mainly focus on marine ecosystems (Wang et al., 2014; Le et al., 2014; Joan et al., 2015), which are rarely reported in freshwater waters. Therefore, the study of freshwater bodies has important theoretical and practical significance, especially with lakes as the research object. However, fresh water has a special nature because of its ecological environment, including the complexity and variability of biological factors in freshwater bodies, organisms, and the environment, which determine the study complexity in freshwater bodies. Therefore, the current study clarifies the compositions of phytoplankton community and their relationship with environmental factors in four urban lakes (Cihu Lake, East Lake, Qingshan Lake and Qinggang Lake) with different eutrophication levels in Huangshi City or Wuhan City, Hubei Province, China by using high-performance liquid chromatography (HPLC)-chemical taxonomy (CHEMTAX) and redundancy analysis (RDA). Materials and methods Study area and sampling strategy Cihu Lake (30012’ N, 11503’ E) is located in the center of Huangshi City, Hubei Province, China. Three sampling points (Fig. 1A) are set up according to the morphological characteristics, geographical location, and pollution degree of Cihu Lake. East Lake (30033’ N, 114023’ E) is located in Wuchang District, Wuhan City, Hubei Province, China, which composed of Guozhen Lake, Fruit Lake, Tangling Lake, Tuan Lake, Hou Lake, Xiaotan Lake, Luoyan and other small sub-lakes. Guozheng APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 18(4):5891-5905. http://www.aloki.hu ● ISSN 1589 1623 (Print) ● ISSN 1785 0037 (Online) DOI: http://dx.doi.org/10.15666/aeer/1804_58915905 © 2020, ALÖKI Kft., Budapest, Hungary Xie et al.: Characteristics of size-fractionized phytoplankton and their response to environmental factors in typical lakes of southeastern Hubei Province, China - 5893 - Lake is the main lake district of East Lake, where three sampling points are also set up (Fig. 1B). Qingshan Lake is located in the northern part of Cihu Lake, which consists of four sub-lakes: fish pond, attached lake 1, main lake district, and attached lake 2, we selected representative main lake district located in Qingshan Lake Park for sampling, three sampling points were set up (Fig. 1C). Three sampling points are also set up in Qinggang Lake, which is located east of Qingshan Lake and southwest of the Tiger’s Head (Fig. 1D). The GPS latitude and longitude of each monitoring point are shown below (Table 1). The sampling times were March 2016, August 2015, November 2015, and January 2016 for spring, summer, autumn, and winter, respectively. Sampling once per season, a total of four lakes were sampled, each lake set up three sampling points. At each sampling point, 900 mL of surface water is collected with plexiglass sampler at a depth of 0.5 m. Determination of photosynthetic pigment contents of the size-fractionized phytoplankton A 900 mL water sample was collected at each sampling point and divided into three equal parts. The first water sample was directly filtered with a 0.7 μm GF/F filter membrane to obtain phytoplankton above 0.7 microns. The second water sample was first filtered with a 20 μm sieve, and then the filtrate was collected and filtered with a 0.7 μm GF/F filter membrane for store to obtain phytoplankton of 0.7 to 20 microns. The third water sample was first filtered with a pore size of 5 μm, and then the filtrate was collected and filtered with a 0.7 μm GF/F filter membrane for store to obtain phytoplankton of 0.7 to 5 microns. Figure 1. Location of the sampling sites in the study area APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 18(4):5891-5905. http://www.aloki.hu ● ISSN 1589 1623 (Print) ● ISSN 1785 0037 (Online) DOI: http://dx.doi.org/10.15666/aeer/1804_58915905
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