A Novel Sesterterpenoid, Nitiol, As a Potent Enhancer of IL-2 Gene
1344 Communications to the Editor Chem. Pharm. Bull. 47(9) 1344—1345 (1999) Vol. 47, No. 9 2 A Novel Sesterterpenoid, Nitiol, as a Nitiol (1), in the form of colorless amorphous, [a]D 39.8 ° (c51.37, CHCl ), gave a molecular ion at m/z 356 (M)1 in Potent Enhancer of IL-2 Gene 3 electron-impact ionization (EI) mass spectrometry, and high- Expression in a Human T Cell Line, resolution EIMS determined the molecular formula C25H40O from the Peruvian Folk Medicine ([M]1 356.3077, Calcd 356.3079). The IR (3422 cm21) spec- trum indicated the presence of hydroxyl group and the UV “Hercumpuri” (Gentianella nitida) (238 nm, log e 3.90) spectrum showed a conjugated diene 1 3) ,a a moiety in the molecule. The H-NMR spectrum of 1 exhib- Nobuo KAWAHARA,* Masato NOZAWA, b b ited 39 nonexchangeable protons, including two tertiary (d Atsuyo KURATA, Takashi HAKAMATSUKA, 0.92 and 1.76) and three secondary (d 0.87, 0.90, and 1.13) a a Setsuko SEKITA, and Motoyoshi SATAKE methyl groups, and three olefinic protons (d 5.36, 5.58 and 13 4) National Institute of Health Sciences (NIHS),a Kamiyoga 1–18–1, 5.63). The C-NMR spectrum of 1 displayed five methyls, Setagaya-ku, Tokyo 158–8501, Japan, and Faculty of Pharmaceutical eight methylenes, eight methines, and four quaternary car- Sciences, Tokyo Science University,b 12 Funakawara-machi, Ichigaya, bons, including an oxygenated carbon (d 59.2) and six Shinjuku-ku, Tokyo 162–0826, Japan. olefinic carbons (d 122.3, 124.3, 131.1, 135.5, 137.1 and Received May 31, 1999; accepted July 2, 1999 149.8).
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