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An International Journal Nature and Science ISSN 1545-0740 Volume 8 - Number 2 (Cumulated No. 35), February 1, 2010 Marsland Press P.O. Box 21126, Lansing, Michigan 48909, USA 525 Rockaway PKWY, #B44, Brooklyn, New York 11212, USA http://www.sciencepub.net http://www.sciencepub.org [email protected] [email protected] 347-321-7172 http://www.sciencepub.net [email protected] Nature and Science ISSN 1545-0740 Nature and Science ISSN: 1545-0740 The Nature and Science is an international journal with a purpose to enhance our natural and scientific knowledge dissemination in the world under the free publication principle. Papers submitted could be reviews, objective descriptions, research reports, opinions/debates, news, letters, and other types of writings that are nature and science related. All manuscripts submitted will be peer reviewed and the valuable papers will be considered for the publication after the peer review. The Authors are responsible to the contents of their articles. 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References should include all the authors’ last names and initials, title, journal, year, volume, issue, and pages etc. ISSN: 1545-0740 ã 2003-2011 Marsland Press http://www.sciencepub.net [email protected] Nature and Science ISSN: 1545-0740 Volume 8 - Number 2 (Cumulated No. 35), February 1, 2010 Cover Page, Introduction, Contents, Call for Papers, All in one file Welcome to send your manuscript(s) to: [email protected]. CONTENTS No. Titles / Authors Full Text Optimization of Asparaginase Production by Pseudomonas Aeruginosa Using Experimental Methods Full Text R. Manikandan1, CN Pratheeba2, Pankaj Sah3 and Fathimunnisa Begum4 1 Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, TN, India 2 Department of Chemical Engineering, Kalasalingam University, TN, India 3&4 Department of Applied Sciences, Higher College of Technology, Muscat, Sultanate of Oman [email protected], [email protected] Abstract: Evaluation of fermentation process parameter interactions for the production of l-asparaginase by 1 Pseudomonas aeruginosa. Box-Behnken design of experimentation was adopted to optimize nutritional sources, physiological (incubation period) and microbial (inoculum level).The experimental results and software predicted enzyme production values were comparable. Incubation period, inoculum level and nutritional source (soybean) were major influential parameters at their individual level. Interaction data of the selected fermentation parameters could be classified as least and most significant at individual and interactive levels. All selected factors showed impact on l-asparaginase enzyme production by this isolated microbial strain either at the individual or interactive level. Incubation temperature, inoculums concentration, and nutritional source (soybean) had impact at individual level. Significant improvement in enzyme production by this microbial isolate was noted under optimized environment. [Nature and Science. 2010;8(2):1-6]. (ISSN: 1545-0740). Key words: box-Behnken; pseudomonas aeruginosa; L- asparaginase; response surface Stability Analysis of Seed Germination and Field Emergence Performance of Tropical Rain-fed Sesame Full Text Genotypes Adebisi, Moruf Ayodele Department of Plant Breeding and Seed Technology, University of Agriculture, P. M. B. 2240, Abeokuta, Ogun State, Nigeria. Tel.: +2348035842319. Email: [email protected] Abstract: The work was carried out to determine the stability of two seed quality traits (seed germination and field emergence) in 14 sesame genotypes that were grown in three plant population environments in Abeokuta, southwest Nigeria in each of two seasons. Seeds harvested from each environment were tested for these quality 2 traits. Data obtained were subjected to analysis of variance of Finlay-Wilkinson regressions and stability analysis. Each genotype was defined by three stability parameters: (1) mean seed germination and field emergence over all environments, (2) the linear regression (b values) of genotype mean seed germination and field emergence in each environment, (3) the mean square deviation from the regression for each genotype (S2d value). The genotypes varied considerably in the two seed quality traits and genotype x environment (GxE) interactions were significant. Regression coefficients ranged from 0.19 to 1.70 for seed germination and 0.14 to 3.01 for field emergence. Genotype 530-6-1 with a regression coefficient close to unit (b=1.03), smaller S2d value and a relatively high seed germination of 79% had general adaptability and somehow averagely stable. The highest field emerging genotypes proved less stable and selection solely for high emergence could result in discarding many genotypes that were relatively better adapted to environmental changes. Genotypes 530-6-1, i 73A-11 and C-K-2 were identified as desirable for seed production in all the three plant population environments. Genotypes 69B-88Z, Domu and 73A-97 were identified as desirable genotypes for cultivation in 133,333 plants ha–1 environment, C-K-2 in 166,667 plants ha–1 environment and 93A-97, 73A-11, 69B-88Z and C-K-2 in 266,667 plants ha–1 environment to obtain seed of high and stable germination and emergence. These genotypes were superior in seed quality and therefore deserve a place in commercial seed production and future seed