FALL 2008 Volume 1, Issue 3 EDITORS: ANDY ALLEN Extension Viticulturist THE MIDWEST WINEGROWER
[email protected] REBECCA FORD QUARTERLY NEWSLETTER Extension Enologist virus indicator grapevines, V. vinifera ‘Cabernet IN THIS ISSUE: Grapevine fanleaf virus, Tomato Franc’, V. vinifera ‘Baco Blanc’, and hybrid ‘LN- GRAPEVINE 1 33’. Vein-clearing appeared on all grafted vines F ANLEAF VIRUS, ringspot virus and Grapevine although grafted LN-33 showed very mild vein- T OMATO RINGSPOT rupestris stem-pitting associated clearing. No visible symptoms were observed on VIRUS AND GRAPE- virus are present in Chardonnay herbaceous plants Chenopodium quinoa, Nico- VINE RUPESTRIS tiana benthamiana, cucumber, tomato, pepper, STEM -PITTING ASSO- with a severe vein-clearing dis- and cowpea after mechanical inoculation with CIATED VIRUS ARE ease leaf sap of diseased Chardonnay vines. This new disease of Chardonnay is tentatively named as PRESENT IN CHAR- ‘grapevine vein-clearing disease’. DONNAY WITH A Shaista Lunden1, Baozhong Meng2, John Avery, SEVERE VEIN- Jr.1 and Wenping Qiu1 ELISA tests failed to detect the nepo- CLEARING DISEASE 1 Department of Agriculture, Missouri State Uni- viruses Tomato ringspot virus (ToRSV), Tobacco versity, Mountain Grove, MO 65711, USA GRAPEVINE VIRUS 3 ringspot virus, Arabis mosaic virus, and peach 2 Department of Molecular and Cellular Biology, SURVEY UNDERWAY rosette mosaic virus as well as Grapevine University of Guelph, 50 Stone Road East, IN MISSOURI leafroll-associated virus 3. Through reverse- Guelph, Ontario, Canada N1G 2W1 transcription polymerase chain reaction (RT- A METHOD FOR 3 PCR) using degenerate primers for detecting A severe disease was observed on the SCREENING FOR H2S grapevine nepoviruses, we detected the presence grape variety Vitis vinifera ‘Chardonnay’ in a two AND MERCPTANS of Grapevine fanleaf virus (GFLV)-specific -acre vineyard in Missouri (Qiu et al.