Nitrate, Nutrient Content and Growth Parameters of Komatsuna (Brassica Rapa L.) in Response to Manure Application Depending on EMN (Estimated Mineralizable Nitrogen)
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Journal of Plant Nutrition ISSN: 0190-4167 (Print) 1532-4087 (Online) Journal homepage: https://www.tandfonline.com/loi/lpla20 Nitrate, nutrient content and growth parameters of komatsuna (Brassica rapa L.) in response to manure application depending on EMN (estimated mineralizable nitrogen) Kyi Moe, Seinn Moh Moh, Aung Zaw Htwe & Takeo Yamakawa To cite this article: Kyi Moe, Seinn Moh Moh, Aung Zaw Htwe & Takeo Yamakawa (2019) Nitrate, nutrient content and growth parameters of komatsuna (Brassicarapa L.) in response to manure application depending on EMN (estimated mineralizable nitrogen), Journal of Plant Nutrition, 42:15, 1726-1739, DOI: 10.1080/01904167.2019.1643366 To link to this article: https://doi.org/10.1080/01904167.2019.1643366 Published online: 23 Jul 2019. Submit your article to this journal Article views: 27 View Crossmark data Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=lpla20 JOURNAL OF PLANT NUTRITION 2019, VOL. 42, NO. 15, 1726–1739 https://doi.org/10.1080/01904167.2019.1643366 Nitrate, nutrient content and growth parameters of komatsuna (Brassica rapa L.) in response to manure application depending on EMN (estimated mineralizable nitrogen) Kyi Moea,b, Seinn Moh Moha, Aung Zaw Htwea,b, and Takeo Yamakawac aPlant Nutrition Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan; bDepartment of Agronomy, Yezin Agricultural University, Nay Pyi Taw, Myanmar; cPlant Nutrition Laboratory, Division of Molecular Biosciences, Department of Biosciences & Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan ABSTRACT ARTICLE HISTORY We investigated the effects of poultry manures (PM) and cow manures Received 22 August 2018 (CM) using estimated mineralizable nitrogen (EMN) method on nitrate, Accepted 25 October 2018 nutrient content and growth parameters of komatsuna and compared with KEYWORDS chemical fertilizer (CF) in an abandoned soil. We applied manures as EMN Estimated-mineralizable-N based on their total N content. The 100% of EMN by PM or CM enhanced (EMN); growth-parameters; dry matter (DM) but depressed nitrate content. The PM-Keifun (PMK) with komatsuna; manures; total N (4.87%) produced greater growth parameters, DM, nutrient content nitrates; nutrient-content and lower nitrate content but manures with total N (<2%) could not. The solo CF150 did not promote leaf number but increased nitrate and Na content. In conclusion, a higher total N (>4%) concentration of manure led to increase availability and nutrient contents, DM, and depressed nitrate content of komatsuna, comparable with solo CF in an abandoned soil. 1. Introduction In modern vegetable production, inorganic fertilizer is exclusively used to maximize the produc- tion of foliage. The application of an excessive amount of chemical fertilizer (CF) decreases the performance of plants because of soil acidification, reduction of soil biological activity, decreased soil physical properties, and lack of micronutrients in fertilizer (Ededirant et al. 2004). In most of cases, CF application causes environmental pollution and ecological problems, which increased production costs (Barth, De Tullio, and Conklin 1998). The source of nutrients and rate of fertilizer used can affect nutrient composition and quality of crops, especially leafy vegetables (Riahi et al. 2009). In green vegetables, fertilizer application not only influences the vegetative growth of the crop but also changes quality aspects such as nitrates, vitamin and antioxidant activities (Seung and Adel 2000). Nitrogen in plant cells can be in the form of nitrate or ammonium. Using CF, nitrate could be postulated to produce detrimental effect to human health (Winchester, Huskins, and Ying 2009). However, without CF, it is not possible to fulfill consumer demand for increased growth or yield of vegetables. Wise use of CF is necessary, especially for vegetable production with minimal negative effects. Organic manures are alternative sources for chemical fertilizer substitution. Organic manures contain higher nitrogen (N), phosphorus (P), potassium (K), and trace element contents. In addition, crops grown with organic manures resulted in a higher total CONTACT Kyi Moe [email protected] Plant Nutrition Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. ß 2019 Taylor & Francis Group, LLC JOURNAL OF PLANT NUTRITION 1727 Table 1. Mineralization efficiency of manures. Mineralization (%) of nutrients from applied manures Total N content (DW) NP2O5 K2O <2% 20 100 65 2–4 % 30 100 65 4% 50 100 65 Data source: Nishio (2007); DW ¼ Dry Weight basis. antioxidant capacity in cabbage (Bimova and Pokluda 2009) and vegetables (Ramesh, Shivana, Santa, and Ram 2011); more vitamin C, iron, magnesium, and phosphorus in fruits, vegetables, and grains; and a significantly lower nitrate content than crops grown with CF (Worthington 2001). On the other hand, an excessive amount of organic manure should not be applied to crops (Liang et al. 2003) because it risks the toxic effects of reduced metabolic intermediates. Miah (1994) reported that only using organic sources could not provide the nutritional requirements of crops due to the slow release of plant nutrients from organic matter. When used appropriately, manure has nutritive and economic values (Khaliq, Abbasi, and Hussain 2006). Manures contain a large amount of nitrogenous compounds, which are easily mineralized to ammonia or nitrate (Jose and Michael 2012). Then, applications of manures stimulate macro- and micro-nutrient uptake, such as N, P, K, Ca, Mg, Fe, Mn, Zn, and Cu in crops (Adesemoye, Torbert, and Kloepper 2010). Thus, one potential way to decrease the negative environmental impacts and nutrient losses resulting from extreme use of CF is application of manures and a supplemental CF, to enhance macro-and micro-nutrient contents and the productivity of komatsuna. Commonly, many researchers apply manures on a weight basis and never consider the total nutri- ent (NPK) content or the mineralizable nutrients in them. Consequently, the nutrient demand of crops is not fully met by the applied manures. Because the manure has to undergo mineralization – process following utilized by the plant. A plant can get only mineralizable N (i.e., NH4 Nand – NO3 N converted from organic N by different soil microbes). Nutrient mineralization from applied manure depends on soil temperature, soil moisture, soil properties, manure characteristics, and micro- bial activity (Eghball et al. 2002). Since these environmental factors are difficult to predict for crop cultivation, an estimate of the rate of mineralization is useful for crop cultivation (Pettygrove, Heinrich, and Crohn 2009). Nishio (2007) reported that the amount of mineralizable nutrients in manure depends on its total N content (Table 1). In this experiment, we calculated the amount of three poultry manures (PM) and three cow manures (CM) (Table 2) based on the estimated minera- lizable N (EMN) dependent on their total N according to Nishio (2007). Importantly, few researchers have considered the application of manures by EMN method with a supplemental CF. Thus, we hypothesized that the EMN from manures could supply enough N to komatsuna without adverse effects. Application of PM or CM in different quantities by EMN and two levels of the supplemental CF was intended to provide a greater advantage than that of CF alone in terms of growth parameters and DM of komatsuna (Brassica rapa L. var. wakana komatsuna) in an abandoned soil. This study also aims to evaluate the contents of nitrates, N, P, K, Ca, Mg, and Na in komatsuna for the safety of consumer in response to different PM or CM application by EMN and compare with solo CF. 2. Materials and methods 2.1. Experimental site A pot experiment was conducted in a glasshouse at Kyushu University, Japan (33370N, 130250E, 3 m above the sea level) from April to May (spring season) in 2017 (day length 13:28–14:10 h; temperature range 15–20 C min, 22–35 C max). 1728 K. MOE ET AL. Table 2. Weight of manure, total NPK and EMN applied from manures and a supplemental chemical fertilizer. Total NPK applied from manure and CF (g pot–1) Mineralizable N (g pot–1) Manure applied –1 No. Treatments (g pot )DW NP2O5 K2O Manures (EMN) CF 1 NPK0 0.00 0.00 0.00 0.00 0.0 0.00 2CF50 0.00 0.25 0.25 0.25 0.0 0.25 3CF100 0.00 0.50 0.50 0.50 0.0 0.50 4CF150 0.00 0.75 0.75 0.75 0.0 0.75 5CF100PMH50 22.28 1.33 0.99 0.87 0.25 0.50 6CF100PMK50 10.27 1.00 0.73 0.61 0.25 0.50 7CF100PME50 38.58 1.33 2.39 1.28 0.25 0.50 8CF100CMG50 34.87 1.33 0.83 0.76 0.25 0.50 9CF100CMN50 40.65 1.33 0.85 0.80 0.25 0.50 10 CF100CMH50 39.87 1.33 0.91 0.76 0.25 0.50 11 CF50 PMH100 44.56 1.92 1.22 0.99 0.50 0.25 12 CF50 PMK100 20.53 1.25 0.72 0.47 0.50 0.25 13 CF50 PME100 77.16 1.92 4.04 1.82 0.50 0.25 14 CF50 CMG100 69.74 1.92 0.92 0.78 0.50 0.25 15 CF50 CMN100 81.30 1.92 0.96 0.86 0.50 0.25 16 CF50 CMH100 79.74 1.92 1.07 0.76 0.50 0.25 Subscript numbers of treatments show the amount of N or EMN applied as a percentage based on 0.5 g N potÀ1.DW¼ Dry weight basis, CF ¼ Chemical fertilizer, EMN ¼ Estimated mineralizable N. 2.2. Experimental design and treatments In a randomized complete block design (RCB) with three replications, three types of PM; Hakkou keifun (PMH), Keifun (PMK), and Ekono hakkou keifun (PME) and three types of CM; Gyufun (CMG), Neobi-ru (CMN), and Hakkou gyufun taihi (CMH) were integrated with either 100% or 50% CF.