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Office of Water EPA-822-R-07-010 4304T December 2016 Public Review Draft Field-Based Methods for Developing Aquatic Life Criteria for Specific Conductivity U.S. Environmental Protection Agency Office of Water, Washington, DC DRAFT December 2016 DO NOT CITE OR QUOTE Public Review Draft PUBLIC REVIEW DRAFT Field-Based Methods for Developing Aquatic Life Criteria for Specific Conductivity U.S. Environmental Protection Agency Office of Water Office of Science and Technology Washington, DC ii CONTENTS LIST OF TABLES ....................................................................................................................... viii LIST OF FIGURES .........................................................................................................................x LIST OF ABBREVIATIONS AND ACRONYMS .................................................................... xiv NOTICES .......................................................................................................................................xv FOREWORD ............................................................................................................................... xvi AUTHORS, CONTRIBUTORS, AND REVIEWERS .............................................................. xvii EXECUTIVE SUMMARY ......................................................................................................... xix GLOSSARY ............................................................................................................................... xxii 1. INTRODUCTION AND BACKGROUND ....................................................................... 1-1 2. PROBLEM FORMULATION ........................................................................................... 2-1 2.1. PROBLEM IDENTIFICATION ................................................................................ 2-1 2.2. STRESSOR OF CONCERN―SALTS ..................................................................... 2-2 2.2.1. Sources of Ions ............................................................................................... 2-3 2.2.2. Conceptual Model .......................................................................................... 2-8 2.2.3. Environmental Transport and Fate of Ions in the Aquatic Environment ....... 2-9 2.3. MEASURE OF EXPOSURE ................................................................................... 2-11 2.4. NATURE OF THE EFFECT ................................................................................... 2-13 2.5. MECHANISMS AND MODES OF ACTION ........................................................ 2-16 2.5.1. Physiological Mechanisms........................................................................... 2-17 2.5.2. Mortality, Growth, and Reproduction .......................................................... 2-19 2.5.3. Emigration.................................................................................................... 2-19 2.5.4. Failure to Recruit ......................................................................................... 2-19 2.5.5. Community Interactions............................................................................... 2-20 2.6. ASSESSMENT ENDPOINTS AND MEASURES OF EFFECT ........................... 2-20 2.6.1. Assessment Endpoints ................................................................................. 2-20 2.6.2. Measures of Effect ....................................................................................... 2-22 2.7. SELECTION OF A FIELD-BASED METHOD ..................................................... 2-25 3. ANALYSIS PLAN: FIELD-BASED METHODS TO DEVELOP SPECIFIC CONDUCTIVITY CRITERIA ........................................................................................... 3-1 3.1. DERIVING A CRITERION CONTINUOUS CONCENTRATION (CCC) ............ 3-1 3.1.1. Establishing the Data Set ............................................................................... 3-4 3.1.1.1. Information Sources ........................................................................ 3-4 3.1.1.2. Selection and Adequacy of Data Sets ............................................. 3-5 3.1.1.3. Quality Assurance/Quality Control (QA/QC) .............................. 3-10 3.1.2. Calculating Genus Extirpation Concentrations (XC95) ................................ 3-11 3.1.2.1. Assigning Qualifying Designation to Extirpation Concentration (XC95) Values ........................................................ 3-14 3.1.3. Calculating the Community-Level Effect Estimate Hazardous Concentration (HC05) ................................................................................... 3-15 3.1.3.1. Validating the Effect Estimate Hazardous Concentration (HC05) by Bootstrapping ............................................................... 3-16 iii CONTENTS (continued) 3.1.4. Assessing Seasonality, Life History, and Sampling Methods ..................... 3-18 3.2. DERIVING A CRITERION MAXIMUM EXPOSURE CONCENTRATION (CMEC) ................................................................................................................... 3-20 3.3. ESTIMATION OF CRITERIA DURATION .......................................................... 3-22 3.3.1. Summary of Recommended Duration for Criterion Continuous Concentration (CCC) and Criterion Maximum Exposure Concentration (CMEC)........................................................................................................ 3-25 3.4. ESTIMATION OF CRITERIA FREQUENCY....................................................... 3-25 3.4.1. Recovery Rates in Literature Reviews ......................................................... 3-27 3.4.2. Life History Considerations ......................................................................... 3-29 3.4.3. Summary for Field-Based Frequency for Criterion Continuous Concentration (CCC) and Criterion Maximum Exposure Concentration (CMEC)........................................................................................................ 3-30 3.5. ASSESSING CAUSATION .................................................................................... 3-30 3.6. ASSESSING WATERBODY APPLICABILITY ................................................... 3-32 3.6.1. Stream pH .................................................................................................... 3-32 3.6.2. Waterbody Type........................................................................................... 3-32 3.7. METHODS FOR APPLICATIONS TO NEW AREAS ......................................... 3-35 3.7.1. Application within an Ecoregion―Background Matching ......................... 3-36 3.7.1.1. Obtaining a Data Set ..................................................................... 3-40 3.7.1.2. Estimating Background Specific Conductivity (SC) .................... 3-40 3.7.1.3. Background-Matching Approach.................................................. 3-41 3.7.1.4. Options When Background in New Area is Different than in Original Area ................................................................................ 3-42 3.7.1.5. Summary of Background Matching Method ................................ 3-42 3.7.2. Developing a Criterion Using Background-to-Criterion Regression Method ......................................................................................................... 3-43 3.7.2.1. Using Background to Calculate a Hazardous Concentration th of the 5 centile (HC05) of a Taxonomic Extirpation Concentration Distribution (XCD) ............................................... 3-46 3.7.2.2. Formula for Calculating the Hazardous Concentration of the 5th centile of a Taxonomic Extirpation Concentration Distribution (HC05) from the Background-to-Criterion Model .... 3-48 3.7.2.3. Formula for Calculating the Lower and Upper 10% Prediction Limits........................................................................... 3-48 3.7.2.4. Criterion Continuous Concentration (CCC) with <200 Paired Biological Data ............................................................................. 3-50 3.7.2.5. Criterion Continuous Concentration (CCC) with 200 to 500 Paired Biological Data .................................................................. 3-50 3.7.2.6. Calculation of the Criterion Maximum Exposure Concentration (CMEC) ................................................................. 3-50 3.7.2.7. Summary ....................................................................................... 3-50 iv CONTENTS (continued) 4. CASE STUDY I: EXAMPLE USING EXTIRPATION CONCENTRATION DISTRIBUTION (XCD) METHOD IN A LOW BACKGROUND SPECIFIC CONDUCTIVITY ECOREGION ...................................................................................... 4-1 4.1. DATA SET CHARACTERISTICS ........................................................................... 4-1 4.1.1. Background Specific Conductivity ................................................................ 4-6 4.1.2. Ionic Composition .......................................................................................... 4-9 4.1.3. Seasonal Specific Conductivity Regime ...................................................... 4-11 4.2. RESULTS ................................................................................................................ 4-12 4.2.1. Extirpation Concentration (XC95) and Hazardous Concentration (HC05) Values (Example Criterion Continuous Concentration [CCC]) .................. 4-12 4.2.2. Example Criterion Maximum Exposure Concentration