Ramularia Vacciniicola Fungal Planet Description Sheets 303

302 Persoonia – Volume 38, 2017

Ramularia vacciniicola Fungal Planet description sheets 303

Fungal Planet 585 – 20 June 2017 Ramularia vacciniicola Crous & Thangavel, sp. nov.

Etymology. Name refers to Vaccinium, the host genus from which this Notes — The genus Ramularia is linked to Mycosphaerella fungus was collected. sexual morphs (Videira et al. 2015a, b). However, the older Classiﬁcation — Mycosphaerellaceae, Capnodiales, Dothi name Ramularia was selected over that of Mycosphaerella (Kirk deomycetes. et al. 2013, Wijayawardene et al. 2014, Rossman et al. 2015) for these fungi. Braun (1998) treated two Ramularia spp. known Mycelium consisting of hyaline, smooth, septate, branched, from Vaccinium. Ramularia vacciniicola is easily distinguished 1.5–2 μm diam hyphae. Conidiophores micronematous, re from R. vaccinii based on its smaller conidial dimensions (USA, duced to conidiogenous cells. Conidiogenous cells erect on hy- conidia ellipsoid-ovoid, subcylindrical-fusoid, 10–20 × 2–5 μm). phae, subcylindrical, straight, hyaline, smooth, 3–10 × 1.5–2.5 Conidia of R. multiplex are also somewhat larger (USA, conidia μm; scars thickened, darkened, somewhat refractive, 0.5 μm ellipsoid-ovoid, subcylindrical-fusoid, 6–15 × 1.5–5 μm), and diam. Secondary ramoconidia hyaline, smooth, subcylindri- further distinct in that the latter species forms well-developed cal to narrowly fusoid-ellipsoid, 0(–1)-septate, 10–20(–30) × fascicles with conidiophores. Furthermore, R. vacciniicola is 2–2.5 μm, with 1–3 apical hila, thickened, darkened, somewhat also phylogenetically distinct from all species presently known refractive, 0.5–1 μm diam. Conidia in branched chains (–8), from culture (see Videira et al. 2016). Based on a megablast hyaline, smooth, guttulate, aseptate, narrowly fusoid-ellipsoid, search using the ITS sequence of the ex-type strain, the best (5–)8–10(–11) × (2–)2.5(–3) μm; hila thickened, darkened, match was to R. proteae (GenBank NR_145097; Identities = somewhat refractive, 0.5–1 μm diam. 524/526 (99 %), no gaps), followed by R. stellenboschensis Culture characteristics — Colonies flat, spreading, with (GenBank NR_145101; Identities = 520/526 (99 %), no gaps). sparse aerial mycelium and even, lobate margins, reaching Based on a megablast search using the actA sequence of the 20 mm diam after 2 wk at 25 °C. On MEA surface dark violet, ex-type strain, the best match is R. stellenboschensis (GenBank reverse sepia. On PDA surface and reverse dark violet. On OA KX287798; Identities = 568/575 (99 %), no gaps), followed surface dirty white with diffuse dark violet pigment in agar. by R. rumicicola (GenBank KX287786; Identities = 543/576

Typus. New Zealand, Rotorua, on Vaccinium sp. (Ericaceae), 2015, (94 %), 2 gaps (0 %)). Based on a megablast search using R. Thangavel (holotype CBS H-23100, culture ex-type CPC 29365 = the his3 sequence of the ex-type strain, the best match was to T15_05165F = CBS 142537 = ICMP 22047, ITS, LSU, actA, his3, and R. proteae (GenBank KX288939; Identities = 374/376 (99 %), tef1 sequences GenBank KY979774, KY979829, KY979862, KY979881, no gaps), followed by R. stellenboschensis (GenBank KX288966; and KY979905, MycoBank MB820963); CPC 29366 = CBS 142537, Identities = 370/376 (98 %), no gaps). CPC 29367–29368, ITS, LSU, actA, his3, and tef1 sequences GenBank KY979775–KY979777, KY979830–KY979832, KY979863–KY979865, KY979882–KY979884, and KY979906–KY979907.

Colour illustrations. Blueberry Cottage berry farm, New Zealand; conidio phores sporulating on PNA; conidiophores and conidial chains. Scale bars = 10 µm.

Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: [email protected] & [email protected] Raja Thangavel, Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland 1140, New Zealand; e-mail: [email protected]