Curriculum Vitae Apurva Patel
Personal Information
Date and place of Birth: 16th January, 1989, Surat (India) Address: A-201, Shilalekh Apartment, Opp. Green arcade, L.P. Savani road, Adajan-pal, Surat- 395009, Gujarat, India Phone: +91-8758695547 Current Lab address: Department of Biotechnology, Veer Narmad South Gujarat University, Surat- 395007, Gujarat, India.
E-mail: [email protected]
Education and Training
Jan 2015- April 2019 Ph.D in Biotechnology (Biosciences), Department of Biotechnology, Veer Narmad South Gujarat University, Surat (India). PhD dissertation thesis title- ‘Apoptotic induction and molecular analysis of Tribulus terrestris plant extract on Human Breast Cancer cells MCF-7’
Jun 2007- Jun 2012 M.Sc. in Biotechnology (+B.Sc., 5 year Integrated course) with score- CGPA- 5.20/6.00, Department of Biotechnology, Veer Narmad South Gujarat University, Surat (India).
Jun 2007 Intermediate (Higher Secondary) with score- 77.40 % from Gujarat Higher Secondary Education Board, India.
Jun 2005 Secondary School with score- 85.57 % Gujarat Higher Secondary Education Board, India.
Lab Experience Jan 2013- April 2014 Lab Technician at SN Gene Pvt ltd. and research center, Surat (India). My Job profile encompasses molecular diagnosis of infectious and genetic diseases and standardization in cytogenetic based and molecular biology based tests, in areas of hematology, pharmacogenetics, and Cancer Biology.
Jan 2012- April 2012 Master’s thesis titled ‘Molecular analysis of functional, phylogentic & Heavy metal resistant genes by culture dependent & culture-independent approach from Lonar lake’ at National Center for Cell Science (NCCS), Pune (India). This assignment included isolation and molecular characterization of heavy metal (Hg) resistant bacteria form Lonar lake, India. The project allowed me to expertise in metagenomic analysis and molecular biology techniques like PCR (gradient, multiplex, RT-PCR, touch-down and touch up PCR), Cloning (TA cloning), PFGE, DGGE, sequencing and sequence analysis.
Research Interest Molecular analysis of apoptotic induction in Human Cancer cells and Plant pharmacological analysis
Didactic activities Supervision of experimental activities of post graduate students Supervised projects- Hala Shaikh “Antimicrobial, Radical Scavenging and Cytotoxicity Studies of Saponin Rich Plants” Khusboo Vora “Evaluation of Antioxidant and Cytotoxic Activity of Medicinal Plants” Femida Patel “In-vitro Cytotoxic Activity of Plant Saponin Extracts on Breast Cancer Cell-Line” Vishma Shah- “Expression analysis of key candidate genes for salinity tolerance from halophytes Prosopis spp.”
Teaching Experience Current Position Teaching assistant at Department of Biotechnology, Veer Narmad South Gujarat University, Surat, India (Joined from 7th October, 2019) Aug 2018 – Oct 2019 Ad-hoc Lecturer at Department of Biotechnology, Shri Ramkrishna Institute of Computer Education and Applied Sciences, Surat (India). Jun 2018- Aug 2018 Ad-hoc Lecturer at at G.N. Pandya Science and Commerce college, Bhestan, Surat (India)
Jun 2014- April 2018 Guest Lecturer at Department of Biotechnology, in Veer Narmad South Gujarat University till date. I have teaching experience to both M. Sc. And B. Sc students and have taught subjects like Dairy Technology, Microbiology, Bioprocess technology and cell and tissue culture.
Technical Skills Research expertise in metagenomics and molecular biology techniques including - o Molecular cloning and recombinant DNA techniques o Gene expression analysis using real-time qPCR and semi-quantitative Reverse Transcriptase PCR. o Automated DNA and RNA (Human and Viral) isolation on Qiagen Qiasymphony and Qiacube o Cell cycle analysis using Flowcytometry, Immunocytochemistry using Flourescent Microscope o Protein analysis by SDS-PAGE and HR-LCMS o Cytotoxicity studies on mammalian cells (including mesenchymal stem cells) using MTT, LDH, XTT, SRB and Colony counting methods o Apoptotic studies on human cancer cells using TUNEL assay, DNA fragmentation assay, caspase 3 colorimetric assay o Plant pharmacological analysis and purification of targeted compounds using column chromatography, HPTLC, HPLC, HR-LCMS Well-versed hands on animal tissue-culture and have experience in maintaining cancer cell line likes MCF-7, U-937, Hela, A-549, ZR-75-1, NCL-H23, Mesenchymal stem cells (adipose-derived), Peripherical Blood mononuclear cells (PBMCs) Reviewing and writing scientific documents. Reviewer for Scientific Reports. Excellent grasp on basic computer programs for data and image collection and processing (MS-Office tools, CorelDRAW graphics suite etc.). Bioinformatics knowledge: Docking studies using AutodockVina, genetic sequence alignments, analysis of gene and protein sequences. Statistical analysis- one way Anova, student t test, Pearson’s correlation and regression
Language Skills Mother tongue – Gujarati English language proficiency – Listening (7), reading (6.5), writing (6.5) and spoken (7) level – C1. (IELTS score – 7.0/9.0 (2019) Other languages- Hindi Oral presentations
Oral presentation at International Symposium on Emerging Biological Trends in 21st Century (Nov, 2017) organized by School of Sciences, P.P.Savani University, Kosamba (India) with abstract titled “Evaluation of Secondary metabolites, Antioxidant Potential and Cytotoxicity of different extracts of Tribulus terrestris” Oral presentation at 4th International Congress of the Society for Ethanopharmocology, India: Healthcare in 21st century: “perspectives of Ethanopharmocology and medicinal plant research” (Feb, 2017) at Uka-tarsadia University, bardoli, (India) with abstract titled ‘“Preliminary studies of apoptosis induced in-vitro anticancer activity of Saponins extracts of Tribulus terrestris against human breast cancer cells” Poster presentations
Poster presentation at Biological tools for Sustainable Environment (BTSE 2014) held at Department of Biosciences, Veer Narmad South Gujarat University, Surat (India) and abstract titled “Bioremediation through Mushroom Cultivation”. Poster presentation at current trends in Biotechnology & business Idea” organized by South Gujarat Chamber of commerce and Industry (SGCCI, 2011) and abstract titled “Evaluation of antifungal and antibacterial activity of marine actinomycetes”. Poster presentation at International science Congres (Dec, 2011) held at Indore (India) and abstract titled “Diversity of endophytes from mangrove Avicennia marina from sea cost of south Gujarat”.
Prize/s
Won the prize for the best Oral presentation at Biological tools for sustainable development (BTSE 2016) held at Department of Biosciences, Veer Narmad South Gujarat University, Surat (India) with abstract titled “Preliminary study on effect of Tribulus terrestris Saponins on proliferation of Adipose derived Mesenchymal Stem cells”
Publication/Manuscript 1. Patel Apurva, Soni Anjali, Siddiqi Nikkhat and Sharma Preeti. An insight into the anticancer mechanism of Tribulus terrestris extracts on human breast cancer cells. 3Biotech. 2019; 9:58. 2. Patel Apurva, Soni Anjali and Sharma Preeti. Effect of Tribulus terrestris Saponins on proliferation of Adipose-derived Mesenchymal Stem Cells. Journal of Cellular Biochemistry. 2018; 1-5. 3. Parekh Smita, Patel Apurva, Soni Anjali and Sharma Preeti. Preliminary Phytochemical Screening and Quantitative Analysis of crude extracts of Nyctanthes arbor-tristis Indigenous to South Gujarat region. International Journal of Life Sciences Research. 2018; 6(3): 25-33. (ISSN 2348-3148). 4. Patel Apurva, Soni Anjali and Sharma Preeti. In vitro cytotoxicity studies of Indian herb Tribulus terrestris on Human Breast Cancer Cells. IOSR-Journal of Biotechnology and Biochemistry. 2016; 2(7). Volume 2, Issue 7, Nov-Dec 2016. 5. Patel Apurva, Soni Anjali and Sharma Preeti. The Protective effects of Plant Saponins on Stem cells: A Review. Anti-Cancer Agents in Medicinal Chemistry, Bentham Science. (Manuscript in communication- BMS-ACAMC-2018-41). 6. Patel Apurva, Soni Anjali and Sharma Preeti. Identification of steroidal saponins from Tribulus terrestris and their in silico docking studies. (Manuscript in preparation)
National and state Level Exam Qualified 1. Gujarat State Eligibility test (GSET) for Assistant Professor held on 30th September, 2018 in the subject of Life Sciences. 2. Indian Council of Agricultural Research (ICAR) National Eligibility test (NET) for assistant professor (2018) with 50.89% in Agriculture Biotechnology discipline. 3. Graduate Aptitude test in Engineering (GATE) (2013) in the subject Biotechnology with 447 GATE score, All India Rank- 1265, and percentile 92.22%.
References Dr. Preeti Sharma Assistant Professor Department of Biotechnology, Veer Narmad South Gujarat University Surat (India) Phone no- +91 9106525136 Email- [email protected]
Dr. Gaurav Shah HOD, Department of Biotechnology, Veer Narmad South Gujarat University, Surat (India). Phone No. +91 9825135299 E mail: [email protected]
Dr. Jay Bergi HOD, Department of Biotechnology, Shri Ramkrishna Institute of Computer Education and Applied Sciences, Surat (India). Phone No. +91 9825803767 E mail: [email protected]
Dr. Nikhat J. Siddiqi Associate Professor Department of Biochemistry, College of Science, King Saud University, Riyadh 11495, Saudi Arabia Phone no- 00966-11-8051348 Email- [email protected], [email protected]