Epitope Tag Antibodies for Epitope Tag Antibodies Flow Cytometry Epitope tag antibodies are not only for western blotting and & Affinity Gels immunoprecipitation! These antibodies can also complement your flow cytometry workflow by facilitating the identification of cells For Purification & Research Applications based on the expression of your transfected protein. BioLegend offers antibodies to HA, DYKDDDDK, His, and GFP epitope tags for flow cytometry applications. For a comprehensive list of available Easy Purification formats, refer to the table on the reverse side. Tagging proteins of interest expands detectability of recombinant HA-Tag Transfected Cells Identified by Intracellular Flow Cytometry proteins. By incorporating an epitope tag into the sequence of a recombinant protein, antibodies can be used for surveillance of the tagged fusion protein. BioLegend has a wide range of antibodies and affinity gels
for many epitope tags, including Myc, His, DYKDDDDK, GST, ell mbe GFP, and HA. The epitope tag antibodies are extremely specific and provide outstanding performance for protein purification, western blot, flow cytometry, and immunoprecipitation. For el i e your convenience, many of our epitope tag antibodies can be purchased immobilized on agarose beads as an affinity matrix or affinity gel for simple purification. Our affinity gels have been og l o e cence n en i tested to be reusable at least five times. CHO-K1 cells (open histogram) or HA tag stably transfected cells (filled histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then intracellularly Stable Activity of anti-c-Myc Agarose Over Ten Rounds of stained with HA.11 Epitope Tag (Clone 16B12) PE (Cat. No. 901527). Affinity Purification . Outstanding Controls . Never doubt your epitope tag antibodies with the Posi-Tag . Epitope Tag Protein (Cat. No. 931301). Posi-Tag represents proteins fused in the following order: GST, T7, HSV, c-Myc, VSV, . Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His. It can be . utilized as a positive control in western blotting experiments . using many of the most common epitope tags and has a molecular weight of approximately 45 kD. .