Product Data Sheet Anti-GFAP

Version: 1 Revision Date: 2015-03-27

Catalog # / Size: 840001 / 100 µl Previously: Covance Catalog# PRB-571C Clone: Poly28400 Isotype: Rabbit Polyclonal Immunogen: This antiserum was made with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with purified GFAP from bovine spinal cord. Reactivity: Human, Mouse, Rat, Cat Preparation: Serum Concentration: Lot-specific (please contact technical support for concentration and total µg amount) Rat cortical neurons and glia in mixed tissue culture stained with NF-H (red) Storage: Store at -20°C. Upon initial thawing, apportion into working aliquots and and GFAP (green). The nuclei are store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing stained with Hoescht dye (blue). the antibody. For long-term storage, keep the antibody at -80°C. Photo courtesy of EnCor Biotechnology Inc. Applications: Applications: WB, IF, ICC Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:10,000* • IF: 1:1,000 (when using fluorescent secondary ab) or 1:5,000 (when using peroxidase labeled secondary ab) Application Notes: This antiserum is effective in immunoblotting (WB), immunofluorescence (IF) and immunocytochemistry (ICC). * Predicted MW = 55 kD & may see one at 48 kD (a breakdown product) Application References: 1. Brenner M et al. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat. Genet. 27:117-120, Rat hippocampus stained with rabbit 2001. anti-GFAP, (red, AF568) and chicken 2. Liem RKH et al. Intermediate filaments in nervous tissues. J. Cell Biol. anti-NF-H, (green, AF488). Photo 79:637-745, 1979. courtesy of Molecular Expressions. 3. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 1972.

Description: Glial Fibrillary Acidic Protein (GFAP) was found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kD protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene. All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes. Other Names: Glial Fibrillary Acidic Protein Related Products:Product Clone Application

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