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Neuroscience Research Products Neuroscience Research Products BioLegend is ISO 9001:2008 and ISO 13485:2003 Certified Toll-Free Tel: (US & Canada): 1.877.BIOLEGEND (246.5343) Tel: 858.768.5800 biolegend.com 02-0010-00 World-Class Quality | Superior Customer Support | Outstanding Value Unlocking the biology of the brain is a research With the acquisition of the Covance antibody product imperative that has garnered much attention from portfolio, BioLegend signaled its commitment to public and private entities in recent years. From the enabling legendary discovery within the neuroscience National Football League to the U.S. Department research community. BioLegend is making further of Defense, traumatic brain injury is being heavily investments in the portfolio, becoming a leading investigated. In addition, the BRAIN Initiative and provider of reagents known for quality and innovation, EU Initiative (humanbrainproject.eu) are focused on and helping scientists advance the understanding of innovative research and technology that increases our brain biology, interrogate their pathways, and identify understanding of the brain. Neurodegenerative diseases potential biomarkers that may improve the success already constitute a public health and economic of clinical programs for neurodegenerative diseases. crisis, and as the population ages, the urgent need for better treatments for these diseases is intensified. Protein Aggregation Protein aggregation is a hallmark of neurodegenerative disease pathology (e.g. beta-amyloid plaques in Alzheimer's disease, Lewy bodies in Parkinson's disease, etc). Post-translational modification of key proteins is known to be a critical factor in the pathways that regulate formation, deposition and propagation of these aggregates. BioLegend offers a portfolio of products that set the standard for quality, allowing researchers to detect native and modified forms of key proteins in a wide range of applications. Staining of β-Amyloid, 1-42 (Clone 12F4) on formalin fixed Strong staining of Tau, 157-168 (Clone 2G9.F10) on formalin- Immunostaining of a Lewy body in a substantia nigra paraffin embedded human Alzheimer's brain. fixed paraffin-embedded human Alzheimer's brain. neuron using mAb clone 4D6, which reacts with human, mouse, and rat α-Synuclein. APP/β-Amyloid Antibodies Description Clone Format Size Cat. No. Description Clone Format Size Cat. No. β-Amyloid Poly18058 Purified 50 µg 805801 β-Amyloid, 1-16 Poly8029 Purified 50 µg 802901 β-Amyloid Poly18058 Biotin 50 µg 805803 β-Amyloid, 1-16 M3.2 Purified 200 µl | 1 mL 805701 | 805702 β-Amyloid Poly18268 Purified 200 µl | 500 µl 826801 | 826802 β-Amyloid, 1-38 7-14-4 Purified 50 µg 808601 1 mL 826803 β-Amyloid, 1-38 7-14-4 Biotin 50 µl 808603 β-Amyloid, 1-8 2H4 Purified 200 µl | 500 µl 805301 | 805302 β-Amyloid, 1-40 11A50-B10 Purified 200 µl | 500 µl 805401 | 805402 1 mL 805303 1 mL 805403 β-Amyloid, 1-8 1E11 Purified 200 µl | 500 µl 805201 | 805202 β-Amyloid, 1-40 11A50-B10 Biotin 200 µl | 500 µl 805404 | 805405 1 mL 805203 1 mL 805406 β-Amyloid, 1-10 20.1 Purified 200 µl | 1 mL 806201 | 806202 β-Amyloid, 1-40 11A50-B10 HRP 200 µl 805407 β-Amyloid, 1-11 NAB 228 Ascites 200 µl 835101 β-Amyloid, 1-42 12F4 Purified 200 µl | 500 µl 805501 | 805502 β-Amyloid, 1-16 6E10 Ascites 200 µl | 500 µl 803015 | 803016 1 mL 805503 1 mL 803017 β-Amyloid, 1-42 12F4 Biotin 200 µl | 500 µl 805504 | 805505 β-Amyloid, 1-16 6E10 Alexa 200 µl 803013 1 mL 805506 Fluor® 488 β-Amyloid, 1-42 12F4 HRP 200 µl 805507 β-Amyloid, 1-16 6E10 Biotin 200 µl | 500 µl 803007 | 803008 β-Amyloid, 1-43 9C4/ Purified 200 µl | 500 µl 805601 | 805602 1 mL 803009 Amyloid 1 mL 805603 β-Amyloid, 1-16 6E10 HRP 200 µl 803011 β-Amyloid, 1-43 9C4/ Biotin 200 µl | 500 µl 805604 | 805605 β-Amyloid, 1-16 6E10 LEAF™ 1 mL 803006 Amyloid 1 mL 805606 β-Amyloid, 1-16 6E10 Purified 200 µl | 500 µl 803001 | 803002 β-Amyloid, 17-24 4G8 Ascites 200 µl | 500 µl 800709 | 800710 1 mL 803003 1 mL 800711 β-Amyloid, 1-16 M3.2 Biotin 200 µl | 1 mL 805703 | 805704 β-Amyloid, 17-24 4G8 Purified 200 µl | 500 µl 800701 | 800702 1 mL 800703 2 Customer Service: 858-768-5800 Description Clone Format Size Cat. No. α-Synuclein ELISAs β-Amyloid, 17-24 4G8 Biotin 200 µl | 500 µl 800704 | 800705 Description Size Cat. No. 1 mL 800706 Human α-Synuclein ELISA Kit 1 kit 844101 β-Amyloid, 17-24 4G8 HRP 200 µl 800707 β-Amyloid 337.48 Purified 100 μl 822301 Tau Antibodies Pyroglutamyl (Glu3) Description Clone Format Size Cat. No. β-Amyloid, x-38 BA1-13 Purified 50 µg 825101 Tau Poly8296 Purified 50 µg 829601 β-Amyloid, x-40 139-5 Biotin 50 µl 805903 Tau Tau 2 Purified 100 µl | 500 µl 806701 | 806702 β-Amyloid, x-40 139-5 Purified 50 µg 805901 0N Tau 3H6.H7 Purified 100 µl 823801 β-Amyloid, x-40 29-6 Purified 100 µl 825201 β-Amyloid, x-42 1-11-3 Biotin 50 µl 812103 1N Tau 4H5.B9 Purified 100 µl 823901 β-Amyloid, x-42 1-11-3 Purified 50 µg 812101 2N Tau 71C11 Purified 100 µl | 500 µl 816801 | 816802 β-Amyloid, x-42 BA3-9 Purified 50 µg 825301 4R Tau 5F9 Purified 100 µl 823701 Amyloid Fibril (pan B10AP Purified 50 µl 812201 Tau, 1-100 43D Purified 100 µl | 500 µl 816601 | 816602 reactive) APP LN27 Purified 200 µl | 500 µl 800901 | 800902 Tau, 6-18 Tau 12 Purified 100 µl | 500 µl 806501 | 806502 1 mL 800903 Tau, 20-35 TAU-13 Ascites 500 µl 835201 APP C-Terminal C1/6.1 Purified 200 µl | 1 mL 802801 | 802802 Tau, 157-168 2G9.F10 Purified 100 µl 824601 Fragment APP C-Terminal Poly8250 Purified 50 µg 825001 Tau, 185-195 39E10 Purified 100 µl 814301 Fragment Tau, 185-195 77E9 Purified 100 µl 814401 APP, 61-77 D3B10 Purified 200 µl | 500 µl 806101 | 806102 1 mL 806103 Tau, 210-230 Tau 5 Purified 200 µl | 500 µl 806401 | 806402 1 mL 806403 APP, 573-596 1G6 Biotin 200 µl 806004 APP, 573-596 1G6 Purified 200 µl | 500 µl 806001 | 806002 Tau, 267-278 5C7 Purified 100 µl 814501 1 mL 806003 Tau, 316-355 77G7 Purified 100 µl | 500 µl 816701 | 816702 sAPPα Poly8135 Purified 50 µg 813501 Tau, x-421 C3 Purified 200 µl | 500 µl 806301 | 806302 sAPPβ Poly8134 Purified 50 µg 813401 1 mL 806303 APP/β-Amyloid Recombinant Proteins Tau, 404-421 Tau46 Purified 200 µl | 500 µl 806601 | 806602 Description Size Cat. No. 1 mL 806603 Recombinant Human β-Amyloid Peptide (1-40) 1 mg 843701 Tau Dimethyl (Lys281) 1C9.G6 Purified 100 µl 819501 Recombinant Human β-Amyloid Peptide (1-42) 1 mg 843801 Tau Dimethyl (Lys311) 7G5.F4 Purified 100 µl 819601 Recombinant Human β-Amyloid Scrambled 500 µg 843901 Tau Nitrated (Tyr18) Tau-nY18 Purified 100 µl | 500 µl 829701 | 829702 Peptide (1-40) Tau Nitrated (Tyr29) Tau-nY29 Purified 200 µl | 500 µl 829801 | 829802 Recombinant Human β-Amyloid Scrambled 500 µg 844001 1 mL 829803 Peptide (1-42) Tau Phospho (Ser214) Poly8071 Purified 50 µg 807101 Recombinant Human APP751 25 µg 842601 Tau Phospho (Ser235) Poly8073 Purified 50 µg 807301 Recombinant Human APP770 25 µg 843201 Tau Phospho (Ser396) PHF-13 Purified 100 µl | 1 mL 829001 | 829002 Recombinant Human sAPPbeta (APP751 20 µg 843501 Tau Phospho (Ser396) Poly8074 Purified 50 µg 807401 isoform) Tau Phospho (Ser404) Poly8075 Purified 50 µg 807501 β-Amyloid ELISAs Tau Phospho (Thr181) Poly8068 Purified 100 µl 806801 Description Size Cat. No. Tau Phospho (Thr205) Poly8069 Purified 50 µg 806901 BetaMark™ β-Amyloid x-40 ELISA Kit 1 kit 842101 Tau Phospho (Thr212) Poly8070 Purified 50 µg 807001 (Chemiluminescent) Tau Phospho (Thr231) PHF-6 Purified 100 µl | 1 mL 828901 | 828902 BetaMark™ β-Amyloid x-40 ELISA Kit (TMB) 1 kit 842301 Tau Phospho (Thr231) Poly8072 Purified 50 µg 807201 BetaMark™ β-Amyloid x-42 ELISA Kit 1 kit 842201 (Chemiluminescent) Tau Recombinant Proteins BetaMark™ β-Amyloid x-42 ELISA Kit (TMB) 1 kit 842401 Description Size Cat. No. α-Synuclein Antibodies Recombinant Human Tau-352 (0N3R) 25 µg 842701 Description Clone Format Size Cat. No. Recombinant Human Tau-381 (1N3R) 25 µg 842801 α-Synuclein Syn 204 Ascites 200 µl 838201 Recombinant Human Tau-383 (0N4R) 25 µg 843001 α-Synuclein Syn303 Purified 100 µl 824301 Recombinant Human Tau-410 (2N3R) 25 µg 842901 α-Synuclein 4D6 Purified 200 µl | 500 µl 834301 | 834302 1 mL 834303 Recombinant Human Tau-412 (1N4R) 25 µg 843101 α-Synuclein Phospho P-syn/81A Purified 100 µl 825701 Recombinant Human Tau-441 (2N4R) 25 µg 842501 (Ser129) α-Synuclein, 103-108 4B12/ Purified 200 µl | 500 µl 807801 | 807802 Synuclein 1 mL 807803 α-Synuclein, 115-121 LB509 Purified 200 µl | 500 µl 807704 | 807705 1 mL 807706 α-Synuclein, 115-121 LB509 Purified 200 µl | 500 µl 807701 | 807702 1 mL 807703 Synuclein-α, Nitrated Syn514 Ascites 200 µl 838901 Synuclein-α/β, Syn 202 Ascites 200 µl 836601 130-140 Synuclein-α/β, Syn12 Ascites 200 µl 838801 Nitrated 3 biolegend.com Protein Aggregation/Modification Antibodies Description Clone Format Size Cat. No. Description Clone Format Size Cat. No. AICD Poly8119 Purified 50 µg 811901 PEN2 (C-terminus) Poly18412 Serum 100 µl 841201 Aph-1a, 245-265 (C Poly18231 Purified 100 µl 823101 Pin1 Poly28224 Purified 100 µl 822401 terminus) PME-1 8A6-F8 Purified 100 µl 820901 Aph-1aL/SLoop Poly18232 Purified 100 µl 823201 Presenilin 1 (CO2 Poly18340 Purified 100 µl 834001 BACE1 (CO2 terminus) Poly8402 Serum 100 µl 840201 terminus) BACE1 (CO2 terminus) Poly8401 Serum 100 µl 840101 Presenilin 1 (N NT1 Purified 200 µl | 500 µl 823401 | 823402 terminus) 1 mL 823403 Prion 3F4 Purified 200 µg | 500 µg 800301 | 800302 1 mg 800303 Presenilin 1 (NH2- Poly18111 Purified 100 µl 811101 terminus) CD230 (Prion) 3F4 Ascites 200 µl | 500 µl 800307 | 800308 1 mL 800309 Presenilin 1, 21-80 24-4B5 Purified 200 µl | 500 µl 823301 | 823302 (NH terminus) 1 mL 823303 CD230 (Prion)
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  • Catalogue# Prot-Ra-Int: Purified Recombinant
    4949 SW 41st Blvd. Suites 40 & 50 Gainesville, FL 32608 Tel: (352) 372 7022 Fax: (352) 372 7066 [email protected] Catalogue# Prot-r-a-Int: Purified recombinant human α-internexin Background: Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of four major proteins called NF-L, NF-M, NF-H and α- internexin (1,2). α-internexin is the lowest molecular weight major neurofilament subunit and runs on SDS-PAGE gels at about 66 kDa, with some variability between species. Antibodies to α-internexin are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Some neurons express only α-internexin and no other neurofilament subunit and α-internexin is expressed earlier in development than the other neurofilament subunits (1,2). Antibodies to α-internexin can also be useful in the diagnostics of neurofilament accumulations seen in certain neurological diseases, such as neurofilament inclusion body disease (3). Figure: Coomassie brilliant blue stained SDS- PAGE gel of various recombinant proteins. His- tagged full length human α-internexin, was expressed and purified from E. coli BL21 using immobilized metal affinity chromatography. 1μg of pure protein was run on each lane. The lane indicated with “α-int” contains the α-internexin protein. The other lanes show recombinant His- tagged peripherin (Peri), vimentin (Vim) and neurofilament NF-L (NF-L) as indicated. Protein molecular weight standards are in the first lane and apparent molecular weights are as indicated. Protein Characteristics: A cDNA encoding full length human α-internexin was inserted into an eukaryotic expression vector which adds an N-terminal in frame His-tag.
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