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Bı∙Olegend 2012–2013 Bı∙oLegend 2012–2013 Catalog and Reference Manual A Decade of Discovery Table of Contents Table of Contents Table General Information How to Use This Catalog and Reference Manual . iv Catalog Abbreviations Guide . v Company Profile . vi Ordering Information . viii Custom Solutions Team . x Technical Services . xi Quality and Satisfaction Guarantee . xi Collaborate With Us . xii Terms and Conditions . xii Product Warranties and Use Limitations . xiv Trademarks and Patents . xv Antibody Product Formats . xvi Frequently Asked Questions . xvii Technical Charts & Protocols . 1 Products . 45 Mouse Immunology . 45 Rat Immunology . 159 Human Immunology . 179 Cytokines, Chemokines, and Related Molecules . 317 Mouse Cytokines, Chemokines, and Related Molecules . 325 Rat Cytokines, Chemokines, and Related Molecules . 365 Human Cytokines, Chemokines, and Related Molecules . 373 Bioassay-Validated Recombinant Cytokines . 431 Cell Biology . 455 Secondary Reagents . 551 Isotype Controls . 565 Buffers, Solutions and Media Additives . 573 Index of Catalog Numbers . 579 Master Index . 607 BioLegend Worldwide . Inside Back Cover biolegend.com • [email protected] iii How to Use this Catalog and Reference Manual To locate products quickly and easily, this catalog is organized as follows: Main Table of Contents: Products categorized by broad theme or species of interest (e g. ., Mouse Immunology) Sectional Tables of Contents: Products listed alphabetically by product/antigen name (e .g ., CD54 or ICAM-1) and by area of biology (e .g ., Adhesion Molecules) Note: In the Mouse, Rat, and Human Immunology sections, the first products listed are antibodies to CD antigens, then antibodies are listed alpha- numerically by antigen name . Antibodies to TCRs and MHC antigens (Mouse) are listed at the ends of these sections . Master Index: Lists products by common/antigen names, CD name, and clone name . Index of Catalog Numbers: Locates page number via catalog number . For example, to find an antibody specific for mouse TNF receptor, type II, you can locate it several different ways in the Master Index: ➢➢ Antigen common name TNFRII ➢➢ CD name CD120b ➢➢ Clone name TR75-89 ➢➢ Catalog number 113401 Once you have found a product of interest, the product pages contain information about both the antigen and the antibody, quality control data, and useful application notes . The small boxes on the bottom of the How to Use this Reference Use and Manual to Catalog How page describe the format of the antibody/product, the catalog number, the size, and the price, as below . Format Biotin Catalog No . 100103 100104 Size 50 μg 500 μg Price $65 $225 Species Reactivity: Listed for clones with multi-species reactivity . Additional information can be found on Cross-Reactivity Charts on pages 28 – 31 . If you can’t find something you are looking for, it is probably already in development! So feel free to contact us: [email protected]. For continually updated product listings, visit BioLegend on-line at biolegend.com. iv Toll Free 1.877.BIOLEGEND (246.5343) Catalog Abbreviations Guide Catalog Abbreviations Guide Abbreviations Catalog For your convenience and ease of use, here is a list of abbreviations used throughout this catalog: aa: amino acids ICFC: Intracellular Staining for Flow Cytometry AKP: Alkaline phosphatase IF: Immunofluorescence microscopy APC: Allophycocyanin Ig: Immunoglobulin Av: Avidin IHC: Immunohistochemistry BA: Bioassay IP: Immunoprecipitation BCA: Bicinchoninic acid protein assay kD: Kilodalton Block: Blocking LEAF™: Low endotoxin, azide-free (formulation) C’: Complement mediated cell depletion LPS: Lipopolysaccharide CNS: Central nervous system µg: Microgram Costim: Costimulatory or costimulation mAb: Monoclonal antibody Cy: Cyanine (dye) MHC: Major histocompatibility complex Deplete or Depletion: Cell depletion Neut: Neutralization ELISA: Enzyme-linked immunosorbent assay ng: Nanogram ELISA Capture/Detection: Capture or detection reagent for NK: Natural killer cells enzyme-linked immunosorbent assay PBMC: Peripheral blood mononuclear cells ELISPOT: Enzyme-linked immunosorbent spot assay PE (or R-PE): Phycoerythrin ELISPOT Capture/Detection: Capture or detection reagent pg: Picogram for enzyme-linked immunosorbent spot assay SAv: Streptavidin EU: Endotoxin Unit SDS-PAGE: SDS polyacrylamide gel electrophoresis FA: Functional assay TCR: T cell receptor FC: Flow cytometry TNFRSF: TNF receptor superfamily FITC: Fluorescein isothiocyanate TNFSF: TNF superfamily GPI: Glycosylphosphatidylinositol Treg: T regulatory cells HRP: Horseradish peroxidase WB: Western blotting ICC: Immunocytochemistry biolegend.com • [email protected] v Company Profile Gene Lay, DVM—Founder, President and CEO Co-founder of PharMingen, now BD Biosciences Nan Jiang, Ph.D.—Vice President of Operations ▪ 20+ years experience in antibody reagent industry, from product development, process development to manufacturing . Craig Monell, Ph.D.—Vice President of Sales, Marketing, and Business Development ▪ 18 years in the Biotechnology industry, including leader- ship roles in Marketing, Business Development, R&D, and Project Management at PharMingen/BD Biosciences, Bio- Carta, and Stratagene/Agilent Technologies John Ransom, Ph.D.—Vice President of Research and Development BioLegend develops world-class, cutting-edge antibodies and ▪ 20+ years in Biotechnology, including leadership positions reagents for biomedical research, manufactured in our state- at Advanced Molecular Design, Cytori Therapeutics, Nova- Company Profile Company of-the-art facility in San Diego, CA . Our mission is to acceler- site, and Axiom Biotechnologies ate research and discovery by providing the highest quality Kent Johnson—Chief Financial Officer products at an outstanding value, along with superior cus- ▪ Former CFO of PharMingen, now BD Biosciences tomer service and technical support . ▪ 19 years reagents manufacturing experience, broad ERP Our product areas include cell immunophenotyping, cyto- and M&A experience, California licensed CPA kines and chemokines, adhesion, cancer research, T regu- Xifeng Yang, M.D.—Senior Director of Cell Analysis latory cells, stem cells, innate immunity, cell-cycle analysis, ▪ 20+ years research and development experience in apoptosis, and modification-specific antibodies . All of BioLe- immunology, hematology, and cell biology gend’s reagents are supported by superior customer service and come with a quality guarantee . Our aggressive prod- Shaoquan Ji, DVM/Ph.D.—Vice President of Biomarkers uct development program, through technology licensing, and Immunoassays collaborations, and internal hybridoma development, pro- ▪ Conducted the development and commercialization of duces strategic reagents for use in a variety of applications multiplex biomarker immunoassays at LINCO Research/ including: MILLIPORE Corp . using Luminex xMAP technology ▪ 25 years experience in the areas of nutrition, physiol- ▪ Flow cytometry and mass cytometry ogy, protein chemistry and molecular biology ▪ ELISA and ELISPOT ▪ Immunoprecipitation and ChIP Nancy Huang—Senior Director of International ▪ Western blotting Distribution ▪ Immunofluorescence microscopy ▪ 20+ years experience in Biotech industry in the areas of ▪ Immunohistochemistry Marketing/Sales, Distribution Management, Business ▪ In vitro and in vivo functional assays Development, and Human Resources, including leader- ship role at PharMingen/BD Biosciences . BioLegend offers the broadest selection of fluorochrome con- jugates for multi-color flow cytometry . With our new Custom Solutions Team, BioLegend is well positioned to offer a wide range of custom products and services tailored to our cus- tomers’ specific needs . Incorporated in June 2002, BioLegend is located in San Diego, in the midst of one of the nation’s largest and fastest growing biotechnology clusters . vi Toll Free 1.877.BIOLEGEND (246.5343) Worldwide Offices Zender-Reinhardt, who are able to discuss projects with cus- See Inside Back Cover for Contact Information. tomers, help with multi-color flow cytometry panels, and BioLegend strives to provide quality research reagents and assist in troubleshooting . BioLegend GmbH provides full expert technical support to the scientific community world- service sales and applications support in Germany, allowing wide . We look forward to serving your research needs and customers to get their research done efficiently and at a great becoming your long term research partner . value . Company Profile Company BioLegend Japan KK, in partnership with Tomy Digital Biology Co ., Ltd ., opened in Tokyo in February 2008 . Yoshiro Namai, Managing Director, and Yoko Yano, Marketing and Business Development Manager, lead a highly experienced BioLegend UK Ltd.: In May 2010, BioLegend expanded into and trained technical staff, providing immediate access to the United Kingdom and now sells directly to UK customers BioLegend’s entire portfolio of reagents and the highest through a collaboration with Cambridge Bioscience . This level of customer service and technical support . collaboration has allowed BioLegend to extend its excellent pricing and value to UK customers . Led by Dr . Vijay Mhaiskar, BioLegend Europe, established in October 2008, provides Managing Director, the BioLegend UK team provides sales convenient ordering and quick delivery for our European cus- and technical applications support for researchers in the UK . tomers . We house significant inventory in Europe, allowing for rapid delivery of products to customers throughout Europe and
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