Epitope Tag Antibodies for Epitope Tag Antibodies Flow Cytometry Epitope tag antibodies are not only for western blotting and & Affinity Gels immunoprecipitation! These antibodies can also complement your flow cytometry workflow by facilitating the identification of cells For Purification & Research Applications based on the expression of your transfected protein. BioLegend offers antibodies to HA, DYKDDDDK, His, and GFP epitope tags for flow cytometry applications. For a comprehensive list of available Easy Purification formats, refer to the table on the reverse side. Tagging proteins of interest expands detectability of recombinant HA-Tag Transfected Cells Identified by Intracellular Flow Cytometry proteins. By incorporating an epitope tag into the sequence of a recombinant protein, antibodies can be used for surveillance of the tagged fusion protein. BioLegend has a wide range of antibodies and affinity gels for many epitope tags, including Myc, His, DYKDDDDK, GST, ell Number GFP, and HA. The epitope tag antibodies are extremely specific and provide outstanding performance for protein purification, western blot, flow cytometry, and immunoprecipitation. For Relative C your convenience, many of our epitope tag antibodies can be purchased immobilized on agarose beads as an affinity matrix 0 103 104 105 or affinity gel for simple purification. Our affinity gels have been Log Fluorescence Intensity tested to be reusable at least five times. CHO-K1 cells (open histogram) or HA tag stably transfected cells (filled histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then intracellularly Stable Activity of anti-c-Myc Agarose Over Ten Rounds of stained with HA.11 Epitope Tag (Clone 16B12) PE (Cat. No. 901527). Affinity Purification 0.35 Outstanding Controls 0.3 Never doubt your epitope tag antibodies with the Posi-Tag 0.25 Epitope Tag Protein (Cat. No. 931301). Posi-Tag represents proteins fused in the following order: GST, T7, HSV, c-Myc, VSV, 0.2 Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His. It can be A280 0.15 utilized as a positive control in western blotting experiments 0.1 using many of the most common epitope tags and has a molecular weight of approximately 45 kD. 0.05 0 BioLegend also carries recombinant peptide tags that can be 12345678910 used during competition experiments to ensure specificity AP Round of your antibody or to elute bound fusion proteins from their SDS-PAGE gel image of protein purified from the 1st to 10th rounds by BioLegend anti-c-Myc respective affinity matrix. agarose. First and last lanes contain ladder. There is no loss of binding capacity even after ten rounds of re-use. Posi-Tag Epitope Tag Protein is Recognized by Several Antibodies Sampler Kits and Small Sizes oly9248) Glu -Myc BioLegend understands that developing a new experimental -c Glu- -GST (4C10)-GST (P anti-HA.11anti anti-6-Hisanti- anti anti system can be difficult. To simplify the process we offer Epitope Tag Antibody Sampler Kits for both big and small motifs so you can find which tag works best in your expression system. We also 45 offer a number of antibodies to other epitopes in small sizes of 25 μL and 25 μg for you to try! Epitope Tag Antibody Sampler Kits 31 Description Specificity Cat. No. Epitope Tag Big Motif GST, GFP, mCherry, Thioredoxin 1 699902 Western blot showing several BioLegend epitope tag antibodies and their detection of 1 µg Antibody Sampler Kit of Posi-Tag Epitope Tag Protein. All monoclonal antibodies were used at a 1:1000 dilution. Epitope Tag Small Motif DYKDDDDK, Myc, HA, V5, His 699903 Poly9248 was used at a dilution of 1:50000. Antibody Sampler Kit To learn more, visit: biolegend.com/epitope_tag_products BioLegend is ISO 13485:2003 Certified Toll-Free Tel: (US & Canada): 1.877.BIOLEGEND (246.5343) | Tel: 1.858.768.5800 biolegend.com 07-0035-06 Epitope Tag Antibodies & Affinity Gels Purified Epitope Tag Antibodies Epitope Tag Affinity Matrices/Gels Specificity Clone Size Cat. No. Specificity Clone Size Cat. No. AU1 AU1 25 μL | 200 μL 901905 | 901901 AU1 AU1 1 mL 900601 500 μL 901902 AU5 AU5 1 mL 900701 Poly29031 100 μL 903101 DYKDDDDK L5 500 μL | 2 mL | 5 mL 651501 | 651502 | 651503 AU5 AU5 25 μL | 500 μL 902007 | 902002 GFP Lag-19 1 mL | 2 mL | 5 mL 689302 | 689303 | 689304 Cre Recombinase Poly9080 200 μL 908001 Glu-Glu Glu-Glu 1 mL 900301 DYKDDDDK L5 25 μg | 200 μg 637319 | 637301 HA 16B12 1 mL 900801 500 μg | 1 mg 637302 | 637303 5 mg 637304 His 6-His 1 mL 900501 Poly9024 200 μL 902401 mCherry LaM-4 1 mL 689502 GFP 1GFP63 25 μg | 100 μg 668206 | 668205 Myc 9E10 500 μL 658502 B34 25 μL | 200 μL 902605 | 902601 RNA Polymerase (Softag 1) NT73 1 mL 673601 500 μL 902602 RNA Polymerase (Softag 3) IIB8 1 mL 673701 FM264G 25 μg | 100 μg 338001 | 338002 Positive Control Glu-Glu Glu-Glu 200 μL | 500 μL 901801 | 901802 Description Size Cat. No. GST 4C10 200 μL | 500 μL 901601 | 901602 Recombinant Posi-Tag 50 μL 931301 P1A12 25 μg | 100 μg 640801 | 640802 Epitope Tag Protein HA 16B12 25 μL | 200 μL 901533 | 901513 500 μL | 1 mL 901514 | 901515 Epitope Tag Peptides Poly9023 25 μL | 200 μL 902303 | 902301 Description Size Cat. No. 500 μL 902302 AU1 1 mg 931801 His 6-His 25 μL | 200 μL 906113 | 906101 500 μL 906102 AU5 1 mg 931901 J095G46 25 μg | 100 μg 362601 | 362602 Glu-Glu 1 mg 931502 J099B12 25 μg | 100 μg 652501 | 652502 HA 1 mg 931401 1 mg 652505 His 1 mg | 5 mg 931601 | 931602 Maltose Binding Protein YM-2 200 μL 906901 Myc 1 mg | 5 mg 931701 | 931702 mCherry 8C5.5 25 μg | 100 μg 677701 | 677702 Direct-Blot™ HRP Epitope Tag Antibodies Myc 9E10 25 μg | 100 μg 626801 | 626802 Specificity Clone Size Cat. No. 9E11 100 μL 904401 DYKDDDDK L5 25 μL | 100 μL 637312 | 637311 Poly9063 200 μL | 500 μL 906301 | 906302 GST P1A12 100 μL 640803 RNA Polymerase (Softag 1) NT73 100 μg 662904 HA 16B12 25 μL | 100 μL 901520 | 901519 RNA Polymerase (Softag 3) IIB8 100 μg 668405 His 6-His 25 μL | 100 μL 906109 | 906110 S-Tag BL26881 25 μg 688102 J095G46 25 μL | 100 μL 362613 | 362614 Strep-Tag BL26882 25 μg 688202 J099B12 25 μg | 100 μg 652503 | 652504 T7 Poly29032 100 μL 903201 Myc 9E10 25 μL | 100 μL 626804 | 626803 Thioredoxin 1 4H12A59 25 μg | 100 μg 658901 | 658902 V5 7/4 25 μL | 100 μL 680604 | 680603 V5 Poly29038 25 μL | 100 μL 903802 | 903801 7/4 25 μg | 100 μg 680601 | 680602 VSV-G Poly29039 100 μL 903901 Fluorophore Conjugated Epitope Tag Antibodies for Flow Cytometry and Immunofluorescence Applications Specificity Clone Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC BV421™ PE PE/Cyanine7 PerCP/Cyanine5.5 DYKDDDDK L5 ICC IF IF FC, ICFC FC FC, ICFC FC FC GFP FM264G ICFC ICFC ICFC ICFC ICFC HA.11 16B12 IF, FC, ICC ICC, IF IF, FC ICFC IF ICFC ICFC ICFC His J095G46 ICC ICC ICC ICFC ICFC Alexa Fluor® is a registered trademark of Life Technologies Corporation. Brilliant Violet™ is a trademark of Sirigen Group Ltd. BioLegend is ISO 13485:2003 Certified Toll-Free Tel: (US & Canada): 1.877.BIOLEGEND (246.5343) | Tel: 1.858.768.5800 biolegend.com 07-0035-06.