The Value of Ex Situ Collections for Global Biodiversity Conservation in the Wild by Andrew Mooney B.A
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The value of ex situ collections for global biodiversity conservation in the wild by Andrew Mooney B.A. (Mod.), Zoology, 2016 A thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy School of Natural Sciences (Zoology) Trinity College Dublin The University of Dublin 2021 CC-BY-SA Declaration I declare that this thesis has not been submitted as an exercise for a degree at this or any other university and it is entirely my own work, except where contributions from co-authors and collaborators are explicitly mentioned within the authorship contributions statement accompanying each chapter. I agree to deposit this thesis in the University’s open access institutional repository or allow the Library to do so on my behalf, subject to Irish Copyright Legislation and Trinity College Library conditions of use and acknowledgement. Andrew Mooney ii Summary Despite the best efforts of conservation practitioners global biodiversity is continuing to decline. The role of zoos and aquariums in conserving global biodiversity ex situ has become increasingly important as more species become threatened with extinction. As ex situ conservation resources are limited, evidence-based decision making is required to identify, and prioritise, the management actions necessary to increase the potential of ex situ conservation efforts. The efficacy of ex situ conservation efforts is currently hindered by 1) entrenched taxonomic biases in collection planning and the prioritisation of large, charismatic vertebrates, 2) the unsustainability of ex situ populations due to limited space availability and management practices, and 3) limited considerations of the potential for ex situ collections to conserve and reintroduce genetic variation into populations using biological samples and advanced reproductive technologies. In this thesis I explore the multifaceted contribution of ex situ collections to global biodiversity conservation. I focus on the importance of standardised, globally shared ex situ records, and their potential to inform collection planning, population sustainability and genetic conservation decision-making. In Chapter 2 I addressed the taxonomic bias in collection planning and assessed the importance of large, charismatic vertebrates in driving both visitor attendance and fundraising for conservation in the wild. Using data from >450 zoological collections globally I identified a net positive effect of large charismatic vertebrates on both visitor attendance, and subsequently conservation fundraising. I also revealed that numerous other factors, such as species richness and species uniqueness, play equally important roles. I suggest that the taxonomic bias in collection planning is potentially an effective conservation strategy, but encourage a more creative approach to collection planning and an assessment of the conservation potential of traditionally non-charismatic species. In Chapter 3 I investigated the potential of globally shared zoological records to provide the management insights necessary to increase the sustainability of ex situ populations, utilising 753 ex situ flamingo (Phoenicopteridae) populations as a case study. I both confirm and contradict existing management guidelines, highlighting the potential of globally shared zoological records, and provide species-specific management recommendations to promote the sustainability of ex situ flamingo populations. In Chapters 4 and 5 I highlighted the role of ex situ collections in conserving the genetic diversity of living populations in gene banks, such as the San Diego Zoo Frozen Zoo®, and the potential of iii ex situ samples to contribute to future genetic rescue and de-extinction efforts. In Chapter 4 I revealed that 5.1% of all threatened amphibian, bird, mammal, and reptile species are represented within the San Diego Zoo Frozen Zoo® and that further sampling from within the global zoo and aquarium community could increase this representation to 16.5%. I provide future sampling suggestions based on both sampling opportunities and existing conservation priorities. In Chapter 5 I addressed the taxonomic bias in de-extinction research and prioritised for de-extinction investigation the 122 species of recently extinct plants, incorporating both the feasibility and the probability of reintroduction success. I show that ex situ samples, such as herbarium specimens, are currently available for nearly all recently extinct plant species, each with the potential to provide seeds capable of germination. These studies highlight the importance of ex situ samples in conserving genetic diversity and their potential to reintroduce genetic variation into existing populations, enhancing ecosystem health and stability. This thesis illustrates the important conservation and management insights that can be derived from globally shared ex situ records, providing the recommendations necessary to increase the efficacy of global ex situ conservation efforts. The current rate of biodiversity loss suggests that the importance of ex situ conservation is only going to increase, however the limited resources available mean that evidence-based decision-making is necessary to ensure conservation opportunities are not overlooked and that existing strategies are effective in achieving their goals. This thesis demonstrates the multi-faceted contribution of ex situ collections to global biodiversity conservation and highlights their future potential, conserving not only individual species, but also ecosystems and the services vital to sustaining human civilisation. iv Acknowledgements The last four years have been an incredible journey, filled with so much love and support from everyone around me. This would not have been possible without the support of my supervisors Yvonne Buckley, Dalia Conde and Kevin Healy, for which I am forever grateful. Yvonne, thank you for believing in both me and this project, and giving me the research freedom to follow my own intuition and passions. More than anything you showed me what it means to be a good scientist, professionally and personally, and I have learned so much from you. I could not have asked for a more supportive supervisor and I will forever be grateful to you for this incredible opportunity. To the Zoology Department, I can’t think of a more supportive or creative place that I could have spent the last four years, thank you for making work never feel like work. Andrew Jackson, thank you for your support as part of my Steering Committee and for going along with every crazy idea I suggested. To the Buckley Lab (Maude, Caroline, Andrew, Aoibheann, Annabel and Ruth), thank you for letting me impose my love of large, charismatic vertebrates on all of you all and telling me when my models were terrible. Thanks to the Festive Office, Maureen and Alain, you made sure there was a never a dull day and have helped me in more ways than you can imagine. Also Maureen, thank you for making sure I got fieldwork during my PhD, even if the water was cold. To Paula, my OG, who would have thought that four years would go by so fast. Going on this journey with you has been a roller-coaster, from start to finish, but I can’t think of anyone else who I would be more grateful to have by my side. I feel so lucky to have gotten to know you and to be able to count you as a close friend. Rachel, you may have left Ireland, but you have no idea how thankful I am for every trip to the Equine Centre, every rant over a badger dissection and every Eddie’s, getting to call you a friend means that no matter what, at least I did one thing right. Floriane and Laura, I may have started my PhD teaching you guys, but by the end of it you taught me so much more, thank you guys for all your support and an almost endless supply of gossip. Sam and Jenny, thank you for not judging my taste in cheese or sushi, and for making me appreciate sharks and soundscapes. Nadia, thank you for every late-night call in San Diego and for always reminding me there is a world outside academia. Thank you for your constant friendship and support, you have shown me what ohana really means and I feel so proud to call v you a friend. Shannen, it’s been a long time since we first met at the back of Goldsmith Hall, but thank you for sharing a tent with me in Kenya and for every coffee rant and bee pun since. Michelle, thank you for always making me feel like one of the Kelly’s, having one family is great, but the support of two has meant the world to me. Rita and Johanna, thank you for making me feel like part of the family, even though we were rarely in the same country. Without your technical support and people management skills this journey would have been very different. You made every conference, trip to Odense and staff retreat feel like home, and your friendship is something I will always cherish. Thank you to everyone at Species360 for your dedication to globally shared zoological records. Jim and Elisabeth, thank you for your constant support and excitement over the work I was doing. Josh and Erika, thank you for embracing my weirdness and showing me what American culture really is. To San Diego Zoo Global and Fulbright Ireland, thank you for an incredible academic and cultural opportunity that I will never forget. Thanks to my ICR Family (Julie, Marlys, Ollie, Aparna, Brigid, Katy and Aryn) for welcoming me and showing me what zoo research really looks like. Taylor Swift, thank you for providing the soundtrack to my life and for reminding me that even though there are going to be people who will try to undercut my success or take credit for my accomplishments, that it was me and the people who love me who put me here, and that is the greatest feeling in the world. This is EPIC!! Aaron, meeting and getting to know you has been one of the most challenging and rewarding things I have done over the last four years.