January 25, 2011 u vol. 108 u no. 4 u 1195–1746

Cover image: Pictured is a scanning electron microscope image that shows wild-type Yersinia pseudotuberculosis (green) attached to the surface of cultured HeLa cells. Pathogenic Yersinia species use a type III secretion system (T3SS) to translocate virulence proteins into the cytosol of target cells. Karen Akopyan et al. demonstrate that the putative one-step T3SS translocation can be accomplished in vitro via two steps that are mediated by intermediate extracellular effector proteins. See the article by Akopyan et al. on pages 1639–1644. Image courtesy of Roland Rosqvist, Per Hörstedt, and Karen Akopyan (Umeå University, Umeå, Sweden); and Stefan Gunnarsson (Uppsala University, Uppsala). From the Cover

1639 Translocating virulence proteins 1246 Contaminants in Vietnam’s deep aquifers 1451 Animal models of preeclampsia 1484 Evolving gene promiscuity 1490 Mating behavior and sperm design

Contents INAUGURAL ARTICLES 1201 mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in THIS WEEK IN PNAS Chi Zhang, Taiowa A. Montgomery, Harrison W. Gabel, Sylvia E. J. Fischer, Carolyn M. Phillips, Noah Fahlgren, Christopher M. Sullivan, James C. Carrington, 1195 In This Issue and Gary Ruvkun

1209 Symmetry, asymmetry, and kinetics of silencing establishment in Saccharomyces cerevisiae revealed LETTERS (ONLINE ONLY) by single-cell optical assays Erin A. Osborne, Yasushi Hiraoka, and Jasper Rine E12 Implications of allometry David L. Skole, Jay H. Samek, and Michael J. Smalligan E13 Reply to Skole et al.: Regarding high-resolution carbon stocks and emissions in the Amazon PHYSICAL SCIENCES Gregory P. Asner, Joseph Mascaro, John K. Clark, and George Powell CHEMISTRY 1217 Rare excitatory amino acid from flowers of zonal geranium responsible for paralyzing the Japanese beetle Christopher M. Ranger, Rudolph E. Winter, Ajay P. Singh, COMMENTARIES Michael E. Reding, Jonathan M. Frantz, James C. Locke, and Charles R. Krause 1197 Preeclampsia: Animal models for a human cure Mira Aubuchon, Laura C. Schulz, and Danny J. Schust 1222 Kinetics and mechanistic analysis of an extremely rapid See companion article on page 1451 carbon dioxide fixation reaction Deguang Huang, Olga V. Makhlynets, Lay Ling Tan, 1199 Evolving promiscuously Sonny C. Lee, Elena V. Rybak-Akimova, and R. H. Holm Dan I. Andersson See companion article on page 1484 1228 Nonheme oxo-iron(IV) intermediates form an oxyl radical upon approaching the C–H bond activation transition state Free online through the PNAS open access option. Shengfa Ye and Frank Neese

PNAS u January 25, 2011 u vol. 108 u no. 4 u iii–viii Downloaded by guest on September 25, 2021 1284 Crystallographic and online spectral evidence for role ANTHROPOLOGY of conformational change and conserved water in 1267 Late Pleistocene adult mortality patterns and modern cytochrome oxidase proton pump human establishment Jian Liu, Ling Qin, and Shelagh Ferguson-Miller Erik Trinkaus 1326 Ligand binding to protein-binding pockets with wet and dry regions APPLIED BIOLOGICAL SCIENCES Lingle Wang, B. J. Berne, and R. A. Friesner 1272 Therapeutic enzyme deimmunization by combinatorial T-cell epitope removal using neutral drift COMPUTER SCIENCES Jason R. Cantor, Tae Hyeon Yoo, Aakanksha Dixit, 1234 Morphological change in machines accelerates the Brent L. Iverson, Thomas G. Forsthuber, evolution of robust behavior and George Georgiou Josh Bongard 1278 Stress-induced evolution of Escherichia coli points to original concepts in respiratory cofactor selectivity ENVIRONMENTAL SCIENCES Clément Auriol, Gwénaëlle Bestel-Corre, Jean-Baptiste 1240 Spring temperature change and its implication in the Claude, Philippe Soucaille, and Isabelle Meynial-Salles change of vegetation growth in North America from 1982 to 2006 Xuhui Wang, Shilong Piao, Philippe Ciais, Junsheng Li, BIOCHEMISTRY Pierre Friedlingstein, Charlie Koven, and Anping Chen 1284 Crystallographic and online spectral evidence for role of conformational change and conserved water in 1246 Arsenic pollution of groundwater in Vietnam cytochrome oxidase proton pump exacerbated by deep aquifer exploitation for Jian Liu, Ling Qin, and Shelagh Ferguson-Miller more than a century Lenny H. E. Winkel, Pham Thi Kim Trang, Vi Mai Lan, 1290 Archaeal 3′-phosphate RNA splicing ligase Caroline Stengel, Manouchehr Amini, Nguyen Thi Ha, characterization identifies the missing Pham Hung Viet, and Michael Berg component in tRNA maturation Markus Englert, Kelly Sheppard, Aaron Aslanian, 1474 Widespread crown condition decline, food web John R. Yates III, and Dieter Söll disruption, and amplified tree mortality with increased climate change-type drought 1296 Splitting of H3–H4 tetramers at transcriptionally active Jofre Carnicer, Marta Coll, Miquel Ninyerola, Xavier Pons, genes undergoing dynamic histone exchange Gerardo Sánchez, and Josep Peñuelas Yael Katan-Khaykovich and Kevin Struhl

1302 Structural and mechanistic insight into covalent GEOPHYSICS substrate binding by Escherichia coli 1252 Prediction of a hexagonal SiO2 phase affecting stabilities dihydroxyacetone kinase of MgSiO3 and CaSiO3 at multimegabar pressures Rong Shi, Laura McDonald, Qizhi Cui, Allan Matte, Taku Tsuchiya and Jun Tsuchiya Miroslaw Cygler, and Irena Ekiel

1308 An intrinsically disordered C terminus allows the La PHYSICS protein to assist the biogenesis of diverse noncoding 1256 Violation of the Leggett–Garg inequality with weak RNA precursors measurements of photons Nathan J. Kucera, Michael E. Hodsdon, and Sandra L. Wolin M. E. Goggin, M. P. Almeida, M. Barbieri, B. P. Lanyon, J. L. O’Brien, A. G. White, and G. J. Pryde 1314 Atomic structure of a nanobody-trapped domain- swapped dimer of an amyloidogenic β2-microglobulin variant Katarzyna Domanska, Saskia Vanderhaegen, Vasundara SOCIAL SCIENCES Srinivasan, Els Pardon, Florine Dupeux, Jose A. Marquez, Sofia Giorgetti, Monica Stoppini, Lode Wyns, Vittorio Bellotti, and Jan Steyaert PSYCHOLOGICAL AND COGNITIVE SCIENCES 1262 Oxytocin promotes human ethnocentrism 1320 Importance of single molecular determinants in the fi Carsten K. W. De Dreu, Lindred L. Greer, Gerben A. delity of expanded genetic codes Van Kleef, Shaul Shalvi, and Michel J. J. Handgraaf Alicja K. Antonczak, Zuzana Simova, Isaac T. Yonemoto, Matthias Bochtler, Anna Piasecka, Honorata Czapinska, Andrea Brancale, and Eric M. Tippmann

BIOLOGICAL SCIENCES BIOPHYSICS AND COMPUTATIONAL BIOLOGY

AGRICULTURAL SCIENCES 1234 Morphological change in machines accelerates the evolution of robust behavior fl 1217 Rare excitatory amino acid from owers of zonal Josh Bongard geranium responsible for paralyzing the Japanese beetle Christopher M. Ranger, Rudolph E. Winter, Ajay P. Singh, 1326 Ligand binding to protein-binding pockets with Michael E. Reding, Jonathan M. Frantz, James C. Locke, wet and dry regions and Charles R. Krause Lingle Wang, B. J. Berne, and R. A. Friesner

iv u www.pnas.org Downloaded by guest on September 25, 2021 1331 High-pressure EPR reveals conformational equilibria and 1397 Inducible formation of breast cancer stem cells and their volumetric properties of spin-labeled proteins dynamic equilibrium with non-stem cancer cells via John McCoy and Wayne L. Hubbell IL6 secretion Dimitrios Iliopoulos, Heather A. Hirsch, Guannan Wang, 1337 Auto-inhibitory role of the EF-SAM domain of STIM and Kevin Struhl proteins in store-operated calcium entry Le Zheng, Peter B. Stathopulos, Rainer Schindl, 1403 Bile acid stimulates hepatocyte polarization through Guang-Yao Li, Christoph Romanin, a cAMP-Epac-MEK-LKB1-AMPK pathway and Mitsuhiko Ikura Dong Fu, Yoshiyuki Wakabayashi, Jennifer Lippincott-Schwartz, and Irwin M. Arias 1343 Lipid packing drives the segregation of transmembrane 1409 Suppression of leukemia development caused by helices into disordered lipid domains in PTEN loss model membranes Wei Guo, Suzanne Schubbert, James Y. Chen, Lars V. Schäfer, Djurre H. de Jong, Andrea Holt, Bahram Valamehr, Sherly Mosessian, Hubing Shi, Andrzej J. Rzepiela, Alex H. de Vries, Bert Poolman, Nhi H. Dang, Consuelo Garcia, Mariana F. Theodoro, J. Antoinette Killian, and Siewert J. Marrink Marileila Varella-Garcia, and Hong Wu 1349 Conformation of peptides bound to the transporter 1415 Phosphorylation of glutamyl-prolyl tRNA synthetase by associated with antigen processing (TAP) cyclin-dependent kinase 5 dictates transcript-selective Meike Herget, Christoph Baldauf, Christian Schölz, translational control David Parcej, Karl-Heinz Wiesmüller, Robert Tampé, Abul Arif, Jie Jia, Robyn A. Moodt, Paul E. DiCorleto, Rupert Abele, and Enrica Bordignon and Paul L. Fox 1355 Structural basis for scaffolding-mediated assembly and 1421 Direct evidence for functional smooth muscle myosin II maturation of a dsDNA virus in the 10S self-inhibited monomeric conformation in Dong-Hua Chen, Matthew L. Baker, Corey F. Hryc, Frank airway smooth muscle cells DiMaio, Joanita Jakana, Weimin Wu, Matthew Dougherty, Deanna L. Milton, Amy N. Schneck, Dominique A. Ziech, Cameron Haase-Pettingell, Michael F. Schmid, Wen Jiang, Mariam Ba, Kevin C. Facemyer, Andrew J. Halayko, , Jonathan A. King, and Wah Chiu Jonathan E. Baker, William T. Gerthoffer, and Christine R. Cremo 1361 Natural tri- to hexapeptides self-assemble in water to amyloid β-type fiber aggregates by unexpected 1427 Nrf2-mediated induction of p62 controls Toll-like – α-helical intermediate structures receptor-4 driven aggresome-like induced structure Charlotte A. E. Hauser, Rensheng Deng, Archana Mishra, formation and autophagic degradation Yihua Loo, Ulung Khoe, Furen Zhuang, Daniel W. Cheong, Ken-ichi Fujita, Daisuke Maeda, Qi Xiao, Angelo Accardo, Michael B. Sullivan, Christian Riekel, and Srinivasa M. Srinivasula Jackie Y. Ying, and Ulrich A. Hauser 1433 Brusatol enhances the efficacy of chemotherapy by inhibiting the Nrf2-mediated defense mechanism 1367 Detection of a ternary complex of NF-κB and IκBα with Dongmei Ren, Nicole F. Villeneuve, Tao Jiang, Tongde Wu, DNA provides insights into how IκBα removes NF-κB Alexandria Lau, Henry A. Toppin, and Donna D. Zhang from transcription sites Shih-Che Sue, Vera Alverdi, Elizabeth A. Komives, 1439 Chromosomal amplification of leucine-rich repeat and H. Jane Dyson kinase-2 (LRRK2) is required for oncogenic MET signaling in papillary renal and thyroid carcinomas 1373 Atomic model of a cypovirus built from cryo-EM Brendan D. Looyenga, Kyle A. Furge, Karl J. Dykema, structure provides insight into the mechanism Julie Koeman, Pamela J. Swiatek, Thomas J. Giordano, of mRNA capping Andrew B. West, James H. Resau, Bin T. Teh, Lingpeng Cheng, Jingchen Sun, Kai Zhang, Zongjun Mou, and Jeffrey P. MacKeigan Xiaoxing Huang, Gang Ji, Fei Sun, Jingqiang Zhang, fl and Ping Zhu 1445 Absence of keratin 8 confers a paradoxical micro ora- dependent resistance to apoptosis in the colon Aida Habtezion, Diana M. Toivola, M. Nadeem Asghar, CELL BIOLOGY Greg S. Kronmal, Jacqueline D. Brooks, Eugene C. Butcher, and M. Bishr Omary 1379 CUB-domain–containing protein 1 (CDCP1) activates Src to promote melanoma metastasis Hui Liu, Shao-En Ong, Kwabena Badu-Nkansah, Jeffrey Schindler, Forest M. White, and Richard O. Hynes DEVELOPMENTAL BIOLOGY 1451 Pravastatin induces placental growth factor (PGF) and 1385 WNK1 is required for mitosis and abscission ameliorates preeclampsia in a mouse model Szu-wei Tu, Abhijit Bugde, Katherine Luby-Phelps, Keiichi Kumasawa, Masahito Ikawa, Hiroyasu Kidoya, and Melanie H. Cobb Hidetoshi Hasuwa, Tomoko Saito-Fujita, Yuka Morioka, Nobuyuki Takakura, Tadashi Kimura, and Masaru Okabe 1391 Inositol polyphosphate multikinase is a physiologic See Commentary on page 1197 PI3-kinase that activates Akt/PKB David Maag, Micah J. Maxwell, Douglas A. Hardesty, Katie L. 1456 Intraflagellar transport protein 122 antagonizes Sonic Boucher, Namrata Choudhari, Adam G. Hanno, Jenny F. Ma, Hedgehog signaling and controls ciliary localization Adele S. Snowman, Joseph W. Pietropaoli, Risheng Xu, of pathway components Phillip B. Storm, Adolfo Saiardi, Solomon H. Snyder, Jian Qin, Yulian Lin, Ryan X. Norman, Hyuk W. Ko, and Adam C. Resnick and Jonathan T. Eggenschwiler

PNAS u January 25, 2011 u vol. 108 u no. 4 u v Downloaded by guest on September 25, 2021 1462 Reactive oxygen species are indispensable in ovulation 1209 Symmetry, asymmetry, and kinetics of silencing Ketty Shkolnik, Ari Tadmor, Shifra Ben-Dor, Nava Nevo, establishment in Saccharomyces cerevisiae revealed Dalia Galiani, and Nava Dekel by single-cell optical assays Erin A. Osborne, Yasushi Hiraoka, and Jasper Rine 1468 Embryonic day 9 yolk sac and intra-embryonic hemogenic endothelium independently generate a B-1 1513 High-quality draft assemblies of mammalian genomes and marginal zone progenitor lacking B-2 potential from massively parallel sequence data Momoko Yoshimoto, Encarnacion Montecino-Rodriguez, Sante Gnerre, Iain MacCallum, Dariusz Przybylski, Michael J. Ferkowicz, Prashanth Porayette, W. Christopher Filipe J. Ribeiro, Joshua N. Burton, Bruce J. Walker, Shelley, Simon J. Conway, Kenneth Dorshkind, Ted Sharpe, Giles Hall, Terrance P. Shea, Sean Sykes, and Mervin C. Yoder Aaron M. Berlin, Daniel Aird, Maura Costello, Riza Daza, Louise Williams, Robert Nicol, Andreas Gnirke, Chad Nusbaum, Eric S. Lander, and David B. Jaffe ECOLOGY 1519 MicroRNA-15a and -16-1 act via MYB to elevate fetal 1474 Widespread crown condition decline, food web hemoglobin expression in human trisomy 13 Š  disruption, and amplified tree mortality with Vijay G. Sankaran, Tobias F. Menne, Danilo cepanovic, increased climate change-type drought Jo-Anne Vergilio, Peng Ji, Jinkuk Kim, Prathapan Thiru, Jofre Carnicer, Marta Coll, Miquel Ninyerola, Xavier Pons, Stuart H. Orkin, Eric S. Lander, and Harvey F. Lodish Gerardo Sánchez, and Josep Peñuelas 1525 Inhibition of helicase activity by a small molecule impairs Werner syndrome helicase (WRN) function in the cellular response to DNA damage or replication stress ENVIRONMENTAL SCIENCES Monika Aggarwal, Joshua A. Sommers, Robert H. 1479 Mercury reduction and complexation by natural organic Shoemaker, and Robert M. Brosh, Jr. matter in anoxic environments 1531 A hyperactive piggyBac transposase for Baohua Gu, Yongrong Bian, Carrie L. Miller, Wenming mammalian applications Dong, Xin Jiang, and Liyuan Liang Kosuke Yusa, Liqin Zhou, Meng Amy Li, Allan Bradley, and Nancy L. Craig – EVOLUTION 1537 Pack-Mutator like transposable elements (Pack-MULEs) induce directional modification of genes through fi fi 1484 Arti cial gene ampli cation reveals an abundance of biased insertion and DNA acquisition promiscuous resistance determinants in Escherichia coli Ning Jiang, Ann A. Ferguson, R. Keith Slotkin, Valerie W. C. Soo, Paulina Hanson-Manful, and Damon Lisch and Wayne M. Patrick See Commentary on page 1199 1543 The Drosophila Myc gene, diminutive, is a positive regulator of the Sex-lethal establishment 1490 Mating behavior and the evolution of sperm design promoter, Sxl-Pe Lukas Schärer, D. Timothy J. Littlewood, Andrea Gretchen Kappes, Girish Deshpande, Brett B. Mulvey, Waeschenbach, Wataru Yoshida, and Dita B. Vizoso Jamila I. Horabin, and Paul Schedl

1496 Newly identified and diverse plastid-bearing branch on the eukaryotic tree of Eunsoo Kim, James W. Harrison, Sebastian Sudek, IMMUNOLOGY Meredith D. M. Jones, Heather M. Wilcox, Thomas A. 1549 T-cell receptor ligation induces distinct signaling Richards, Alexandra Z. Worden, and John M. Archibald pathways in naïve vs. antigen-experienced T cells Keishi Adachi and Mark M. Davis 1501 Birth and death of genes linked to σ chromosomal inversion 1555 14-3-3 regulates B-cell homeostasis through Yoshikazu Furuta, Mikihiko Kawai, Koji Yahara, Noriko stabilization of FOXO1 Takahashi, Naofumi Handa, Takeshi Tsuru, Kenshiro Yu-Wen Su, Zhenyue Hao, Atsushi Hirao, Kazuo Yamamoto, Oshima, Masaru Yoshida, Takeshi Azuma, Masahira Wen-Jye Lin, Ashley Young, Gordon S. Duncan, Hiroki Hattori, Ikuo Uchiyama, and Ichizo Kobayashi Yoshida, Andrew Wakeham, Philipp A. Lang, Kiichi Murakami, Pamela Ohashi, and Tak W. Mak 1507 Holocephalan embryos provide evidence for gill arch 1561 Cellular and molecular events in the localization of appendage reduction and opercular evolution in diabetogenic T cells to islets of Langerhans fi cartilaginous shes Boris Calderon, Javier A. Carrero, Mark J. Miller, J. Andrew Gillis, Kate A. Rawlinson, Justin Bell, Warrick S. and Emil R. Unanue Lyon, Clare V. H. Baker, and Neil H. Shubin 1567 Entry of diabetogenic T cells into islets induces changes that lead to amplification of the cellular response Boris Calderon, Javier A. Carrero, Mark J. Miller, and Emil R. Unanue 1201 mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans 1573 TNF activates calcium–nuclear factor of activated Chi Zhang, Taiowa A. Montgomery, Harrison W. Gabel, T cells (NFAT)c1 signaling pathways in Sylvia E. Fischer, Carolyn M. Phillips, Noah Fahlgren, human macrophages Christopher M. Sullivan, James C. Carrington, Anna Yarilina, Kai Xu, Janice Chen, and Gary Ruvkun and Lionel B. Ivashkiv

vi u www.pnas.org Downloaded by guest on September 25, 2021 1579 T-helper cell type 2 (Th2) memory T cell-potentiating 1633 Listeria monocytogenes exploits cystic fibrosis cytokine IL-25 has the potential to promote transmembrane conductance regulator (CFTR) angiogenesis in asthma to escape the phagosome Chris J. Corrigan, Wei Wang, Qiu Meng, Cailong Fang, Andrea L. Radtke, Kelsi L. Anderson, Michael J. Davis, Huifen Wu, Victoria Reay, Ze Lv, Yiqiang Fan, Matthew J. DiMagno, Joel A. Swanson, Yunqing An, Yui-Hsi Wang, Yong-Jun Liu, and Mary X. O’Riordan Tak H. Lee, and Sun Ying 1639 Translocation of surface-localized effectors in type III secretion Karen Akopyan, Tomas Edgren, Helen Wang-Edgren, MEDICAL SCIENCES Roland Rosqvist, Anna Fahlgren, Hans Wolf-Watz, 1585 TNF-α promotes fracture repair by augmenting the and Maria Fallman recruitment and differentiation of muscle-derived fi stromal cells 1645 Vibrio vulni cus rtxA1 gene recombination generates Graeme E. Glass, James K. Chan, Andrew Freidin, toxin variants with altered potency during Marc Feldmann, Nicole J. Horwood, intestinal infection and Jagdeep Nanchahal Jayme S. Kwak, Hee-Gon Jeong, and Karla J. F. Satchell

1591 A CD133-related gene expression signature identifies an aggressive glioblastoma subtype with NEUROSCIENCE excessive mutations 1651 Imaging electrical resonance in hair cells Xiaowei Yan, Li Ma, Danielle Yi, Jae-geun Yoon, Alan Jonathan A. N. Fisher, Lukasz Kowalik, Diercks, Gregory Foltz, Nathan D. Price, Leroy E. Hood, and A. J. Hudspeth and Qiang Tian 1657 Disruption of circadian clocks has ramifications for 1597 Loss-of-function mutation in GATA4 causes anomalies of metabolism, brain, and behavior human testicular development Ilia N. Karatsoreos, Sarah Bhagat, Erik B. Bloss,  Diana Lourenço, Raja Brauner, Magda Rybczynska, John H. Morrison, and Bruce S. McEwen Claire Nihoul-Fékété, Ken McElreavey, and Anu Bashamboo 1663 Discerning nonrigid 3D shapes from motion cues Anshul Jain and Qasim Zaidi 1603 Molecular imaging of active mutant L858R EGF receptor (EGFR) kinase-expressing nonsmall cell lung carcinomas 1669 TrkB receptor controls striatal formation by regulating using PET/CT the number of newborn striatal neurons Hsin Hsien Yeh, Kazuma Ogawa, Julius Balatoni, Uday Maryna Baydyuk, Theron Russell, Guey-Ying Liao, Mukhapadhyay, Asutosh Pal, Carlos Gonzalez-Lepera, Keling Zang, Juan Ji An, Louis French Reichardt, Aleksandr Shavrin, Suren Soghomonyan, Leo Flores II, and Baoji Xu Daniel Young, Andrei Y. Volgin, Amer M. Najjar, 1675 Homeobox genes Gsx1 and Gsx2 differentially regulate Victor Krasnykh, William Tong, Mian M. Alauddin, telencephalic progenitor maturation and Juri G. Gelovani Zhenglei Pei, Bei Wang, Gang Chen, Motoshi Nagao, 1609 Irradiation induces bone injury by damaging bone Masato Nakafuku, and Kenneth Campbell marrow microenvironment for stem cells 1681 Impaired hippocampal spinogenesis and neurogenesis Xu Cao, Xiangwei Wu, Deborah Frassica, Bing Yu, and altered affective behavior in mice lacking Lijuan Pang, Lingling Xian, Mei Wan, Weiqi Lei, heat shock factor 1 Michael Armour, Erik Tryggestad, John Wong, Shusaku Uchida, Kumiko Hara, Ayumi Kobayashi, Chun Yi Wen, William Weijia Lu, Mitsuaki Fujimoto, Koji Otsuki, Hirotaka Yamagata, and Frank J. Frassica Teruyuki Hobara, Naoko Abe, Fumihiro Higuchi, 1615 Thyrotrophin receptor signaling dependence of Tomohiko Shibata, Shunsuke Hasegawa, Satoshi Kida, Braf-induced thyroid tumor initiation in mice Akira Nakai, and Yoshifumi Watanabe Aime T. Franco, Roberta Malaguarnera, Samuel Refetoff, 1687 Social performance reveals unexpected vocal Xiao-Hui Liao, Emma Lundsmith, Shioko Kimura, competency in young songbirds Catrin Pritchard, Richard Marais, Terry F. Davies, Satoshi Kojima and Allison J. Doupe Lee S. Weinstein, Min Chen, Neal Rosen, Ronald Ghossein, Jeffrey A. Knauf, and James A. Fagin PHARMACOLOGY 1693 Augmented glucose-induced insulin release in mice MICROBIOLOGY lacking Go2, but not Go1 or Gi proteins 1621 Copper resistance is essential for virulence of Ying Wang, Sangeun Park, Neil S. Bajpayee, Yoshiko Mycobacterium tuberculosis Nagaoka, Guylain Boulay, Lutz Birnbaumer, Frank Wolschendorf, David Ackart, Tej B. Shrestha, and Meisheng Jiang Laurel Hascall-Dove, Scott Nolan, Gyanu Lamichhane, Ying Wang, Stefan H. Bossmann, Randall J. Basaraba, and Michael Niederweis PHYSIOLOGY 1627 Validation of isoleucine utilization targets in 1699 Ca2+-dependent structural rearrangements within Plasmodium falciparum Na+–Ca2+ exchanger dimers Eva S. Istvan, Neekesh V. Dharia, Selina E. Bopp, Ilya Scott A. John, Bernard Ribalet, James N. Weiss, Gluzman, Elizabeth A. Winzeler, and Daniel E. Goldberg Kenneth D. Philipson, and Michela Ottolia

PNAS u January 25, 2011 u vol. 108 u no. 4 u vii Downloaded by guest on September 25, 2021 1705 Deep bradycardia and heart block caused by inducible 1729 Phytochromes inhibit hypocotyl negative gravitropism cardiac-specific knockout of the pacemaker channel by regulating the development of endodermal gene Hcn4 amyloplasts through phytochrome-interacting factors Mirko Baruscotti, Annalisa Bucchi, Carlo Viscomi, Giacomo Keunhwa Kim, Jieun Shin, Sang-Hee Lee, Hee-Seok Kweon, Mandelli, Giacomo Consalez, Tomaso Gnecchi-Rusconi, Julin N. Maloof, and Giltsu Choi Nicola Montano, Karina Rabello Casali, Stefano Micheloni, Andrea Barbuti, and Dario DiFrancesco PSYCHOLOGICAL AND COGNITIVE SCIENCES 1262 Oxytocin promotes human ethnocentrism PLANT BIOLOGY Carsten K. W. De Dreu, Lindred L. Greer, Gerben A. 1711 Extranuclear protection of chromosomal DNA from Van Kleef, Shaul Shalvi, and Michel J. J. Handgraaf oxidative stress 1735 Spontaneous voice–face identity matching by rhesus Sandy Vanderauwera, Nobuhiro Suzuki, Gad Miller, Brigitte monkeys for familiar conspecifics and humans van de Cotte, Stijn Morsa, Jean-Luc Ravanat, Alicia Hegie, Julia Sliwa, Jean-René Duhamel, Olivier Pascalis, Christian Triantaphylidès, Vladimir Shulaev, Marc C. E. and Sylvia Wirth Van Montagu, Frank Van Breusegem, and Ron Mittler 1717 Arabidopsis decuple mutant reveals the importance of SUSTAINABILITY SCIENCE SnRK2 kinases in osmotic stress responses in vivo Hiroaki Fujii, Paul E. Verslues, and Jian-Kang Zhu 1741 Integrating fisheries approaches and household utility models for improved resource management fl 1723 Selection patterns on restorer-like genes reveal a con ict E. J. Milner-Gulland between nuclear and mitochondrial genomes throughout angiosperm evolution Sota Fujii, Charles S. Bond, and Ian D. Small ix Subscription Form

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