bioRxiv preprint doi: https://doi.org/10.1101/181891; this version posted August 29, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Title Anaerobic methanotrophic communities thrive in deep submarine permafrost Authors Matthias Winkel1,*, Julia Mitzscherling1, Pier P. Overduin2, Fabian Horn1, Maria Winterfeld3, Ruud Rijkers1, Mikhail N. Grigoriev4, Christian Knoblauch5, Kai Mangelsdorf6, Dirk Wagner1, and Susanne Liebner1 Affiliations 1GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 Geomicrobiology, 14473 Potsdam, Germany 2Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Periglacial Research, 14473 Potsdam, Germany 3Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Marine Geochemistry, 27570 Bremerhaven, Germany 4Mel’nikov Permafrost Institute, SB RAS, Yakutsk, 677010 Russia 5Institute of Soil Science, Universität Hamburg, 20146 Hamburg, Germany 6GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 3.2 Organic Geochemistry, 14473 Potsdam, Germany *Corresponding author: Matthias Winkel
[email protected] Telegrafenberg, Building F, room 357 14473 Potsdam bioRxiv preprint doi: https://doi.org/10.1101/181891; this version posted August 29, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic (ANME-2a/b, ANME-2d) assemblages in frozen and completely thawed submarine permafrost sediments.