bioRxiv preprint doi: https://doi.org/10.1101/667535; this version posted June 13, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Reconstruction of the carbohydrate 6-O sulfotransferase gene family evolution 2 in vertebrates reveals novel member, CHST16, lost in amniotes 3 4 5 Daniel Ocampo Daza1,2 and Tatjana Haitina1* 6 7 1 Department of Organismal Biology, Uppsala University, Norbyvägen 18 A, 752 36 8 Uppsala, Sweden. 9 2 School of Natural Sciences, University of California Merced, 5200 North Lake Road, 10 Merced CA 95343, United States. 11 *Author for correspondence: Tatjana Haitina, Department of Organismal Biology, Uppsala 12 University, Norbyvägen 18 A, 752 36 Uppsala, Sweden, Tel.: +46 18 471 6120; E-mail: 13
[email protected] 14 15 Keywords: carbohydrate 6-O sulfotransferases, Gal/GalNAc/GlcNAc 6-O sulfotransferases, 16 whole genome duplication, vertebrate 17 18 Running title: Evolution of carbohydrate 6-O sulfotransferases in vertebrates 1 bioRxiv preprint doi: https://doi.org/10.1101/667535; this version posted June 13, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 19 Abstract 20 Glycosaminoglycans are sulfated polysaccharide molecules, essential for many biological 21 processes.