1 Supplementary Material

2

3 Supplementary Table S1. All investigated and genome/transcriptome assemblies.

4

5 Supplementary Table S2. Neighboring families around C6OST .

6

7 Supplementary Fig. S1. Maximum likelihood phylogeny of CHST1 sequences with full

8 species representation. The phylogeny is supported by approximate Likelihood-Ratio Test

9 (aLRT) and UltraFast Bootstrap (UFBoot) analyses, and rooted with the inshore hagfish

10 CHST1 sequence. Both aLRT and UFBoot supports are shown, in that order. aLRT supports

11 have been rounded to the nearest integer. Sequence names include species name

12 abbreviations (see Supplementary table 1) followed by /linkage group

13 designations (if available) and gene symbols. Asterisks indicate incomplete sequences. The

14 fast-evolving branch of neoteleost CHST1b sequences is circled.

15

16 Supplementary Fig. S2. Maximum likelihood phylogeny of CHST16 sequences with full

17 species representation. The phylogeny is rooted with the cyclostome CHST16 sequences. See

18 Fig. S1 caption for more details.

19

20 Supplementary Fig. S3. Maximum likelihood phylogeny of CHST3 sequences with full

21 species representation. The phylogeny is rooted with the lamprey CHST3 sequences. See Fig.

22 S1 caption for more details.

23

24 Supplementary Fig. S4. Maximum likelihood phylogeny of CHST2 sequences with full

25 species representation. The phylogeny is rooted with the cartilaginous CHST2 sequences

1 26 due to the ambiguous identity of the cyclostome CHST2/7-like sequences. See Fig. S1

27 caption for more details.

28

29 Supplementary Fig. S5. Maximum likelihood phylogeny of CHST2 sequences with full

30 species representation. The phylogeny is rooted with the cartilaginous fish CHST7 sequences

31 due to the ambiguous identity of the cyclostome CHST2/7-like sequences. See Fig. S1

32 caption for more details.

33

34 Supplementary Fig. S6. Alternative conserved synteny between CHST2 and CHST7-

35 bearing chromosome regions in tetrapods and the spotted gar, including CHST2a and

36 CHST2b-bearing regions in teleost .. Stars indicate chromosome regions in common

37 with analysis on figure 4B.

38

39 Supplementary Fig. S7. Maximum likelihood phylogeny of CHST4, CHST5 and related

40 sequences with full species representation. The phylogeny is rooted with the non-avian reptile

41 and amphibian “CHST4/5-like” sequences. See Fig. S1 caption for more details.

42

43 Supplementary Fig. S8. Conserved synteny between the anole lizard “CHST4/5-like” gene-

44 bearing region on chromosome 2 and the corresponding human and spotted gar chromosome

45 regions. The gene predictions located within a segment of 5 MB in each direction of the

46 CHST4/5-like gene were identified as described in Methods, and the orthologous gene

47 locations in the human and spotted gar genomes were identified in the Ensembl database.

48

49 Supplementary Fig. S9. “CHST4/5-like” genes in amphibians and non-avian reptiles.

50 Chromosome positions are given in megabases (Mb).

2 51

52 Supplementary Data S1. Identified C6OST sequence data. Includes chromosomal locations

53 and database identifiers of all C6OST sequences identified in this study.

54

55 Supplementary Data S2. All C6OST sequences identified in this study in alphabetical order

56 by species. Sequence names include species abbreviations (Supplementary table 1) followed

57 by chromosome/linkage group designations (if available) and gene symbols. Asterisks

58 indicate incomplete sequences. Numbers after / indicate sequence length. Also includes a list

59 of identical sequences.

60

61 Supplementary Data S3. Neighboring gene family data. Includes chromosomal locations

62 and database identifiers of all neighboring genes in the human, chicken Western clawed frog,

63 spotted gar, , medaka, elephant shark, vase tunicate and fruit fly. Also includes all

64 identified synteny blocks.

3 1 Supplementary Table S1. All investigated species and genome/transcriptome assemblies. 2 Order Species Common name Abbr. Data type Chr./LG Assembly version Database Link Mammals Monotremata Ornithorhynchus_ana

Ornitorhynchus anatinus Oan Genome No NCBI [LINK] tinus-5.0.1

Ornitorhynchus anatinus Platypus Oan Genome No mOrnAna1_t3.p VGP [LINK] Didelphimorphia Gray short-tailed

Monodelphis domestica Mdo Genome Yes MonDom5 NCBI [LINK] opossum Diprotodontia Phascolarctos cinereus Koala Pci Genome No phaCin_unsw_v4.1 NCBI [LINK] Primates Homo sapiens Human Hsa Genome Yes GRCh38.p12 NCBI [LINK]

Pan troglodytes Ptr Genome Yes Clint_PTRv2 NCBI [LINK]

Macaca mulatta Mml Genome Yes Mmul_8.0.1 NCBI [LINK] Bolivian squirrel

Saimiri boliviensis boliviensis Sbo Genome No SaiBol1.0 NCBI [LINK] monkey

Callithrix jacchus Marmoset Cja Genome Yes Callithrix jacchus-3.2 NCBI [LINK] Rodentia Mus musculus Mouse Mmu Genome Yes GRCm38.p6 NCBI [LINK] Lagomorpha Oryctolagus cuniculus Rabbit Oca Genome Yes OryCun2.0 NCBI [LINK] Eulipotyphla Condylura cristata Star-nosed mole Ccr Genome No ConCri1.0 NCBI [LINK] Chiroptera Common vampire

Desmodus rotundus Dro Genome No ASM294091v2 NCBI [LINK] bat Greater horseshoe

Rhinolophus ferrumequinum Rfe Genome No mRhiFer1_v1 VGP [LINK] bat Cetartiodactyla Physeter catodon Sperm whale Pca Genome Yes PhyCat2.0 NCBI [LINK]

Sus scrofa Pig Ssc Genome Yes Sscrofa11.1 NCBI [LINK] Carnivora Canis lupus familiaris Domestic Cfa Genome Yes CanFam3.1 NCBI [LINK] Proboscidea African savanna

Loxodonta africana Laf Genome No Loxafr3.0 NCBI [LINK] elephant Cingulata Nine-banded

Dasypus novemcinctus Dno Genome No Dasnov3.0 NCBI [LINK] armadillo Birds Rheiformes Rhea americana Greater rhea Ram Genome No rheAme1 NCBI [LINK] Galliformes Helmeted

Numida meleagris Nme Genome Yes NumMel1.0 NCBI [LINK] guineafowl Gallus gallus Chicken Gga Genome Yes GRCg6a NCBI [LINK] Tympanuchus cupido Greater prairie T_cupido_pinnatus_

Tcu Genome No NCBI [LINK] pinnatus chicken GPC_3440_v1

Meleagris gallopavo Turkey Mga Genome Yes Turkey_5.0 NCBI [LINK] Anseriformes Anas platyrhynchos Mallard duck Apl Genome No BGI_duck_1.0 NCBI [LINK] IASCAAS_PekingDu

Anas platyrhynchos Mallard duck Apl Genome Yes NCBI [LINK] ck_PBH1.5

Anser cygnoides Swan goose Acy Genome No GooseV1.0 NCBI [LINK]

Anser brachyrhynchus Pink-footed goose Abr Genome No ASM259213v1 NCBI [LINK] Apodiformes Anna's

Calypte anna Can Genome Yes bCalAnn1_v1 VGP [LINK] hummingbird Cuculiformes Cuculus canorus Common cuckoo Ccn Genome No ASM70932v1 NCBI [LINK] Columbiformes Columba livia Rock pigeon Cli Genome Yes colLiv2 NCBI [LINK]

Patagioenas fasciata monilis Band-tailed pigeon Pfa Genome No NIATT_ARIZONA NCBI [LINK] Charadriiformes Thick-billed

Uria lomvia Ulo Genome No UriLom_1.1 NCBI [LINK] murre/guillemot Sphenisciformes Aptenodytes forsteri Emperor penguin Afo Genome No ASM69914v1 NCBI [LINK]

Spheniscus mendiculus Galápagos penguin Sme Genome No ASM326465v1 NCBI [LINK]

Pygoscelis antarcticus Chinstrap penguin Pan Genome No ASM326459v1 NCBI [LINK] Suliformes Galápagos flightless Nannopterum harrisi cormorant Nha Genome No Pharrisi_ref_V1 NCBI [LINK] Ophistocomiformes Ophistocomus hoazin Hoatzin Oho Genome No ASM69207v1 NCBI [LINK] Accipitriformes European golden

Aquila chrysaetos chrysaetos Ach Genome No bAquChr1.1 NCBI [LINK] eagle Strigiformes Strix occidentalis caurina Spotted owl Soc Genome No Soccid_v01 NCBI [LINK] Piciformes Downy

Picoides pubescens Ppu Genome No ASM69900v1 NCBI [LINK] woodpecker Falconiformes Falco peregrinus Peregrine falcon Fpe Genome No F_peregrinus_v1.0 NCBI [LINK]

Falco cherrug Saker falcon Fch Genome No F_cherrug_v1.0 NCBI [LINK] Psittaciformes Melopsittacus_undula

Melopsittacus undulatus Budgerigar Mun Genome No NCBI [LINK] tus_6.3

Strigops habroptilus Kakapo Sha Genome No bStrHab1_v1 VGP [LINK] Passeriformes Blue-crowned Lepidothrix_coronata

Lepidothrix coronata Lco Genome No NCBI [LINK] manakin -1.0

Corvus cornix cornix Hooded crow Cco Genome No ASM73873v2 NCBI [LINK]

Parus major Great tit Pmj Genome Yes Parus_major1.1 NCBI [LINK]

Ficedula albicollis Collared flycatcher Fal Genome Yes FicAlb1.5 NCBI [LINK] Taeniopygia_guttata-

Taeniopygia guttata Zebra finch Tgu Genome Yes NCBI [LINK] 3.2.4

Taeniopygia guttata Zebra finch Tgu Genome No bTaeGut1_v1 VGP [LINK] Passer_domesticus-

Passer domesticus House sparrow Pdo Genome Yes NCBI [LINK] 1.0 Non-avian Crocodylia Alligator mississipiensis American alligator Ami Genome No ASM28112v4 NCBI [LINK] reptiles Australian

Crocodylus porosus Cpo Genome No CroPor_comp1 NCBI [LINK] saltwater crocodile Testudines Western painted Chrysemys_picta_bel

Chrysemys picta bellii Cpi Genome Yes NCBI [LINK] turtle lii-3.0.3

Chelonia mydas Green sea turtle Cmy Genome No CheMyd_1.0 NCBI [LINK] Chinese soft-shell

Pelodiscus sinensis Psi Genome No PelSin_1.0 NCBI [LINK] turtle Rhynchocephalia Sphenodon punctatus Tuatara Spp Genome No ASM311381v1 NCBI [LINK] Gekkota Paroedura picta Ocelot gecko Ppi Genome No Ppicta_assembly_v1 NCBI [LINK] Laterata European green

Lacerta viridis Lvi Genome No ASM90024590v1 NCBI [LINK] lizard Iguania Carolina anole

Anolis carolinensis Aca Genome Yes AnoCar2.0 NCBI [LINK] lizard Central bearded

Pogona vitticeps Pvi Genome No pvi1.1 NCBI [LINK] dragon Serpentes Habu (Ryukyu

Protobothrops flavoviridis Pfl Genome No HabAm_1.0 NCBI [LINK] Islands pit viper) Common garter Thamnophis_sirtalis-

Thamnophis sirtalis Tsi Genome No NCBI [LINK] snake 6.0 Eastern brown

Pseudonaja texilis Pte Genome No EBS10Xv2-PRI NCBI [LINK] snake Python_molurus_bivi

Python bivittatus Burmese python Pbi Genome No NCBI [LINK] ttatus-5.0.2 Amphibians Gymnophiona Rhinatrema bivittatum Two-lined caecilian Rbi Genome No aRhiBiv1_t4.p VGP [LINK] Caudata AxolotlMexicanum_a Ambysto

Ambystoma mexicanum Axolotl Ame Genome No [LINK] ssembly.v1 ma.org

Ambystoma mexicanum Axolotl Ame Genome No ASM291563v2 NCBI [LINK] Sandberg

Notophthalmus viridescens Eastern newt Nvi Transcriptome N/A Feb 2014 [LINK] lab Anura Nanorana parkeri Parker's slow frog Npa Genome No ASM93562v1 NCBI [LINK] Western clawed

Xenopus (Silurana) tropicalis Xtr Genome Yes JGI v9.1 NCBI [LINK] frog African clawed JGI v9.2/

Xenopus laevis Xla Genome Yes NCBI [LINK] frog Xenopus_laevis_v2 Lobe-finned Actinistia

Latimeria chalumnae Coelacanth Lch Genome No LatCha1 NCBI [LINK] fishes Ray-finned Holostei Amia calva Bowfin Acl Transcriptome N/A N/A PhyloFish [LINK] fishes

Lepisosteus oculatus Spotted gar Loc Genome Yes LepOcu1 NCBI [LINK] Teleosts Osteoglossomorpha African

Pantodon buchholzi Pbu Transcriptome N/A N/A PhyloFish [LINK] butterflyfish Peters'

Gnathonemus petersii Gpe Transcriptome N/A N/A PhyloFish [LINK] elephantnose fish Old Calabar

Paramormyrops kingsleyae Pki Genome No PKINGS_0.1 NCBI [LINK] mormyrid Asian arowana

Scleropages formosus Sfo Genome No ASM162426v1 NCBI [LINK] (golden)

Osteoglossum bicirrhosum Silver arowana Obi Transcriptome N/A N/A PhyloFish [LINK]

Arapaima gigas Arapaima Agi Genome No GCA_900497675.1 NCBI [LINK] Elopomorpha Anguilla anguilla European eel Aan Transcriptome N/A N/A PhyloFish [LINK] Anguilla_anguilla_v1

Anguilla anguilla European eel Aja Genome No NCBI [LINK] _09_nov_10

Anguilla japonica Japanese eel Aja Genome No Ajaponica_ver_D2 NCBI [LINK] Clupeiformes Clupea harengus Atlantic herring Cha Genome No ASM96633v1 NCBI [LINK]

Alosa alosa Allis shad Aal Transcriptome N/A N/A PhyloFish [LINK]

Sardina pilchardus European pilchard Spi Genome No SP_G NCBI [LINK] Danio rerio Zebrafish Dre Genome Yes GRCz11 NCBI [LINK]

Danionella dracula Dracula fish Ddr Genome No fDanDra1.1 NCBI [LINK] Leuciscus waleckii Amur ide Lwa Genome Yes GCA_900092035.1 NCBI [LINK]

Cyprinus carpio Common carp Cca Genome Yes GCA_000951615.2 NCBI [LINK]

Carassius auratus Goldfish Cau Genome Yes ASM336829v1 NCBI [LINK] Characiformes Astyanax_mexicanus

Astyanax mexicanus Mexican cave tetra Amx Genome Yes NCBI [LINK] -2.0 Pygocentrus_nattereri

Pygocentrus nattereri Red-bellied piranha Pna Genome No NCBI [LINK] -1.0.2 Gymnotiformes Ee_SOAP_WITH_SS

Electrophorus electricus Electric eel Eel Genome No NCBI [LINK] PACE Siluriformes Ictalurus punctatus Channel catfish Ipu Genome Yes IpCoco_1.2 NCBI [LINK] Esociformes Esox lucius Northern pike Elu Genome Yes Eluc_V3 NCBI [LINK] Salmoniformes Salmo salar Atlantic salmon Ssa Genome Yes ICSASG_v2 NCBI [LINK]

Salvelinus alpinus Arctic char Sal Genome Yes ASM291031v2 NCBI [LINK]

Oncorhynchus mykiss Rainbow trout Omy Genome Yes Omyk_1.0 NCBI [LINK] Osmeriformes Osmerus eperlanus European smelt Oep Genome No ASM90030227v1 NCBI [LINK] Lampriformes Lampris guttatus Opah Lgu Genome No ASM90030254v1 NCBI [LINK]

Regalecus glesne Giant oarfish Rgl Genome No ASM90030258v1 NCBI [LINK] Gadiformes Gadus morhua Atlantic cod Gmo Genome No GadMor_May2010 NCBI [LINK] Beryciformes Monocentris japonica Pineconefish Mja Genome No ASM90032336v1 NCBI [LINK] Holocentriformes Longspine

Holocentrus rufus Hru Genome No ASM90030261v1 NCBI [LINK] squirrefish Blackbar

Myripristis jacobus Mjc Genome No ASM90030255v1 NCBI [LINK] soldierfish Ophidiiformes Brotula barbata Bearded brotula Bba Genome No ASM90030326v1 NCBI [LINK] Gobiiformes Boleophthalmus Great bluespotted

Bpe Genome No BP.fa NCBI [LINK] pectinirostris mudskipper Periophthalmus

N/D Pmg Genome No PM.fa NCBI [LINK] magnuspinnatus Scombriformes Thunnus albacares Yellowfin tuna Tal Genome No ASM90030262v1 NCBI [LINK] Syngnathiformes Hippocampus comes Tiger tail seahorse Hco Genome No H_comes_QL1_v1 NCBI [LINK] ssc_2016_12_20_chr

Syngnathus scovelli Gulf pipefish Sco Genome Yes Cresko lab [LINK] omlevel Synbranchiformes Zig-zag eel/tire-

Mastacembelus armatus Mar Genome No fMasArm1.1 NCBI [LINK] track eel Anabantiformes Anabas testudineus Climbing perch Ate Genome No fAnaTes1.1 NCBI [LINK] Carangiformes Seriola quinqueradiata Japanese amberjack Squ Genome No Squ_2.0 NCBI [LINK] Centropomidae Lates calcarifer Barramundi Lcl Genome No ASM164080v1 NCBI [LINK] Pleuronectiformes Scophthalmus maximus Turbot Sma Genome Yes ASM318616v1 NCBI [LINK]

Cynoglossus semilaevis Tongue sole Cse Genome Yes Cse_v1.0 NCBI [LINK] Beloniformes Oryzias latipes Medaka Ola Genome Yes ASM223467v1 NCBI [LINK] Cyprinodontiformes Nothobranchius furzeri Turquoise killifish Nfu Genome Yes Nfu_20140520 NCBI [LINK] Guppy_female_1.0+

Poecilia reticulata Guppy Pre Genome No NCBI [LINK] MT X_maculatus-5.0-

Xiphophorus maculatus Southern platyfish Xma Genome Yes NCBI [LINK] male Cichliformes Astatotilapia calliptera Eastern happy Asc Genome Yes fAstCal1.2 NCBI [LINK]

Maylandia zebra Zebra mbuna Mze Genome Yes M_zebra_UMD2a NCBI [LINK]

Oreochromis niloticus Nile tilapia Oni Genome Yes Orenil1.1 NCBI [LINK]

Simochromis diagramma N/D Sdi Genome No fSimDia1.1 NCBI [LINK]

Amphilophus citrinellus Midas cichlid Aci Genome No Midas_v5 NCBI [LINK] Pomacentridae Amphiprion percula Orange clownfish Ape Genome Yes Nemo_v1.1 NCBI [LINK] Pseudochromidae Pseudochromis fuscus Brown dottyback Pfu Genome No ASM90032334v1 NCBI [LINK] Labriformes Symphodus melops Corkfin wrasse Sml Genome No ASM281910v1 NCBI [LINK] Centrarchiformes Oplegnathus fasciatus Barred knifejaw Ofa Genome No ASM341684v1 NCBI [LINK]

Maccullochella peelii Murray cod Mpe Genome No mcod_v1 NCBI [LINK] Perciformes Perca fluviatilis European perch Pfv Genome No UTU_Pfluv_1.1 NCBI [LINK]

Sebastes nigrocinctus Tiger rockfish Sni Genome No ASM47523v3 NCBI [LINK] Three-spined

Gasterosteus aculeatus Gac Genome Yes BROAD S1 Ensembl [LINK] stickleback Moroniformes Dicentrarchus labrax European sea bass Dla Genome No seabass_V1.0 NCBI [LINK] Sciaeniformes Large yellow

Larimichthys crocea Lcr Genome Yes L_crocea_2.0 NCBI [LINK] croaker Spariformes Sparus aurata Gilthead seabream Sau Genome Yes ASM330901v1 NCBI [LINK] Spondyliosoma cantharus Black seabream Sca Genome No ASM90030268v1 NCBI [LINK] Tetraodontiformes Mola mola Ocean sunfish Mmo Genome No ASM169857v1 NCBI [LINK] Japanese pufferfish

Takifugu rubripes Tru Genome Yes FUGU5 NCBI [LINK] (fugu) Green spotted

Tetraodon nigroviridis Tni Genome Yes TETRAODON 8.0 Ensembl [LINK] pufferfish Cartilaginous Holocephala Callorhinchus_milii-

Callorhinchus milii Elephant shark Cmi Genome No NCBI [LINK] fishes 6.1.3 Batoidea Amblyraja radiata Thorny skate Ara Genome No sAmbRad1_p1 VGP [LINK]

Leucoraja erinacea Little skate Ler Transcriptome N/A GSE93582 NCBI [LINK]

Okamejei kenojei Ocellate spot skate Oke Transcriptome N/A Data2Okenojeitrinity Figshare [LINK] Selachimorpha Brownbanded

Chiloscyllium punctatum Cpu Genome No Cpunctatum_v1.0 NCBI [LINK] bambooshark

Rhincodon typus Whale shark Rty Genome No ASM164234v2 NCBI [LINK]

Rhincodon typus Whale shark Rty Genome No Rtypus_kobe_v1.0 Figshare [LINK]

Scyliorhinus torazame Cloudy catshark Sto Genome No Storazame_v1.0 NCBI [LINK] Cyclostomes Myxiniformes Eptatretus burgeri Inshore hagfish Ebu Genome No Eburgeri_3.2 Ensembl [LINK] Petromyzontiformes Arctic (Japanese)

Lethenteron camtschaticum Lca Genome No LetJap1.0 NCBI [LINK] lamprey

Petromyzon marinus Sea lamprey Pma Genome No Pmar_germline 1.0 NCBI [LINK] Tunicates Tunicata Ciona intestinalis Vase tunicate Cin Genome Yes KH NCBI [LINK] Cephalochordates Cephalochordata Branchiostoma floridae Florida lancelet Bfl Genome No Version 2 NCBI [LINK] Hemichordates Enteropneusta Saccoglossus kowalevskii Acorn worm Sko Genome No Skow_1.1 NCBI [LINK] Echinoderms Echinoidea Strongylocentrotus

Purple sea urchin Spu Genome No Spur_4.2 NCBI [LINK] purpuratus Arthropods Diptera Drosophila melanogaster Fruit fly Dme Genome Yes BDGP5 Ensembl [LINK] Hymenoptera Apis mellifera Honey bee Aml Genome Yes Amel_HAv3.1 NCBI [LINK] Lepidoptera Bombyx mori Domestic silkworm Bmo Genome No ASM15162v1 NCBI [LINK] 3 1 Supplementary Table S2. Neighboring gene families around C6OST genes.

Subset Symbol Description Subset 1 ANO1/2 Anoctamin 1 and 2 ANO3/4/9 Anoctamin 3, 4 and 9 ANO5/6/7 Anoctamin 5, 6 and 7 ARFGAP ADP-ribosylation factor GTPase activating C1QTNF4/17 C1q and tumor necrosis factor related protein 4 and 17 CRY Cryptochrome DEPDC4/7 DEP domain containing 4 and 7 LDH Lactate dehydrogenase MYBPC Myosin binding protein C PACSIN Protein kinase C and casein kinase substrate in neurons PPFIBP PPFIA binding protein/Liprin-beta RASSF9/10 Ras association domain family member 9 and 10 Solute carrier family 5, members 5, 6, 8 and 12 (sodium coupled SLC5A5/6/8/12 monocarboxylate transporter) SLC17A6/8 Solute carrier family 17 (organic anion transporter), members 6 and 8 TCP11 T complex 11 Subset 2 DNAJA1/2/4 DnaJ heat shock protein family (Hsp40), members A1, A2 and A4 GLG1 Golgi glycoprotein 1 GPI Glucose-6-phosphate isomerase LSM14A LSM14 homolog A, mRNA processing body assembly factor SLC27A Solute carrier family 27, members 2, 3, 5 and 6 (fatty acid transporter) TRPM1/3/6/7 Transient receptor potential cation channel subfamily M, members 1, 3, 6 and 7 Subset 3 CDHR1 Cadherin related family member 1 DNAJB12 DnaJ heat shock protein family (Hsp40), members B12, B14 and C18 LRIT Leucine-rich repeat, immunoglobulin-like and transmembrane domains PALD1 Paladin PPA Pyrophosphatase (inorganic) 1 and 2 PPIF Peptidyl prolyl isomerase F QRFPR Pyroglutamylated RFamide peptide receptor RGR Retinal G protein-coupled receptor ZMIZ , MIZ-type Subset 4 BRINP BMP/retinoic acid inducible neural specific NCK NCK adaptor protein NOS1AP Nitric oxide synthase 1 adaptor protein PFK Phosphofructokinase liver, muscle and platelet types RGS4/5/8/16 Regulator of G-protein signaling 4, 5, 8 and 16 SLC9A6/7/9 Solute carrier family 9, members 6, 7 and 9 (sodium/hydrogen exchanger) ST6GAL ST6 beta-galactoside alpha-2,6-sialyltransferase ITPR Inositol 1,4,5-triphosphate receptor Subset 5 ABI ABL interactor ACBD4/5 Acyl-CoA binding domain containing 4 and 5 ANXA13 Annexin A 13 (Previous ID: ENSFM00760001714601) DIPK2 Divergent protein kinase domain 2A and 2B TNFR/NGFR cysteine-rich region and calcium-binding EGF-like domain ELCB containing PCOLCE Procollagen C-endopeptidase enhancer PLSCR Phospholipid scramblase Subset6 KLF9/13/14/16 Krueppel-like factor 9, 13, 14 and 16 RPGRIP1 Retinitis pigmentosa GTPase regulator interacting protein 1 2 3 Gene family symbols and descriptions are based on approved HUGO Committee (HGNC) 4 gene symbols and descriptions accessed through http://www.genenames.org. Where only a subset of protein 5 subtypes/isoforms are part of the gene family, the included members are specified and the gene symbol with the 6 smallest numeral is used to designate the gene family. * No HGNC symbols or descriptions are available for 7 ELCB. Gene and family symbols were instead constructed from the EGF-Like Calcium Binding domain found 8 in the sequences of this family. Ebu.CHST1 Cmi.CHST1* 86/100 Sto.CHST1* 96/98 Rty.CHST1 47/89 Ler.CHST1* 100/100 Ara.CHST1 88/100 Oke.CHST1 Lch.CHST1 Ame.CHST1 77/93 Nvi.CHST1 82/93 56/93 Npa.CHST1 100/100 Xtr.4.chst1 99/100 Xla.4L.chst1.L 75/100 99/100 Xla.4S.chst1.S Rbi.CHST1 19/94 Ami.CHST1 Cpo.CHST1 Cmy.CHST1 77/93 Cpi.4.CHST1 93/78 Spp.CHST1 100/100 40/79 Psi.CHST1 41/97 Ppi.CHST1 100/100 Lvi.CHST1 59/100 Aca.1.CHST1 83/78 52/100 Pvi.CHST1 88/100 Pbi.CHST1 99/100 Pfl.CHST1 76/100 Pte.CHST1 77/100 Tsi.CHST1 22/62 Oan.CHST1 100/100 Pci.CHST1 93/100 Mdo.5.CHST1 100/86 75/97 Cja.11.CHST1 Sbo.CHST1 0/77 Ptr.11.CHST1 100/100 0/84 Hsa.11.CHST1 Mml.14.CHST1 0/79 Laf.CHST1 77/80 Mmu.2.Chst1 96/97 Dro.CHST1 0/39 Ssc.2.CHST1 62/77 93/94 Pca.16.CHST1 78/44 Ccr.CHST1 82/99 Cfa.18.CHST1 43/52 Ocu.1.CHST1 78/57 Dno.CHST1 84/99 Rfe.CHST1 Afo.CHST1 0/95 Sme.CHST1 84/100 Pan.CHST1 79/88 Ach.CHST1 94/87 Soc.CHST1 0/79 Lco.CHST1 0/81 Fch.CHST1 95/100 Fpe.CHST1 87/87 Cli.5.CHST1 96/99 Pfa.CHST1 0/88 Pmj.5.CHST1 90/96 Pdo.5.CHST1 0/46 Tgu.5.CHST1 0/22 Cco.CHST1 0/44 Fal.5.CHST1 0/51 Ulo.CHST1 0/25 Oho.CHST1 78/75 Ccn.CHST1 0/18 Ram.CHST1 92/87 Can.5A.CHST1 0/97 Nha.CHST1 0/45 Apl.5.CHST1 96/100 Acy.CHST1 84/100 Abr.CHST1 68/49 Mun.CHST1 92/98 Sha.CHST1 90/83 Ppu.CHST1 75/82 Nme.6.CHST1 96/99 Gga.5.CHST1 70/100 Tcu.CHST1 83/100 Mga.5.CHST1 Spi.CHST1b*2of3( ) 81/67 Sfo.CHST1*2of2b( ) 100/100 99/68 Agi.CHST1*2of2b( ) Cha.CHST1b 37/92 Aal.CHST1b Spi.CHST1b*1of3( ) 76/54 Amx.24.CHST1b 100/100 Pna.CHST1b 98/100 Eel.CHST1b 74/100 Ipu.8.CHST1b 98/100 Cau.50.CHST1b*1of3( ) 100/100 Cau.CHST1b*2of3( ) 77/100 68/70 Cau.CHST1b*3of3( ) 87/70 Lwa.6.CHST1b 78/71 Ddr.CHST1b 61/70 Dre.25.chst1b Spi.CHST1b*3of3( ) 50/71 Sal.4.CHST1b.1 99/100 Ssa.10.CHST1b.1 69/100 Omy.2.CHST1b.1 99/100 Elu.19.CHST1b 57/97 Omy.CHST1b.2 68/94 Ssa.16.CHST1b.2 0/56 Sal.26.CHST1b.2 88/98 Oep.CHST1b

93/98 Rgl.CHST1b Mja.CHST1b 100/100 Mjc.CHST1b 76/98 Squ.CHST1b Pfu.CHST1b 81/99 97/99 Ola.6.CHST1b 89/100 Ape.4.CHST1b 85/100 100/100 Aci.CHST1b 100/100 99/86 Oni.CHST1b 99/100 Mze.7.CHST1b 88/100 Asc.7.CHST1b 35/89 0/75 Sdi.22.CHST1b Mar.CHST1b 88/90 Ate.CHST1b 76/87 Tni.Un.CHST1b 89/90 Cse.6.CHST1b 67/26 Lcl.CHST1b 68/85 Sml.CHST1b 73/61 Sni.CHST1b 0/7 Mpe.CHST1b 75/18 Sca.CHST1b 95/99 Lcr.XXI.CHST1b 33/96 Dla.6.CHST1b Pki.CHST1a 99/100 Gpe.CHST1a 97/95 Pbu.CHST1a 0/54 Agi.CHST1*1of2a 84/79 Sfo.CHST1*1of2a 74/79 Obi.CHST1a 95/97 Loc.27.CHST1 99/100 Acl.CHST1 Aja.CHST1*1of2( ) 99/100 Aan.CHST1*1of2( ) 88/86 Aja.CHST1*2of2( ) 100/100 Aan.CHST1*2of2( ) 43/85 Cha.CHST1a 99/100 Spi.CHST1a 51/100 Aal.CHST1a 90/100 95/91 Eel.CHST1a 99/100 Pna.CHST1a 71/91 Amx.18.CHST1a 82/91 Ipu.4.CHST1a 100/100 Elu.2.CHST1a 97/100 Omy.CHST1a 100/100 Ssa.CHST1a.2 83/100 Ssa.11.CHST1a.1 Oep.CHST1a 96/100 ( ) 94/84 Bpe.CHST1a*1of2 85/100 Pmg.CHST1a*1of2( ) 100/100 Bpe.CHST1a*2of2( ) 100/100 75/97 Pmg.CHST1a*2of2( ) Gmo.CHST1a Pfv.CHST1a 59/81 Dla.5.CHST1a 69/44 Rgl.CHST1a 95/85 100/100 Lgu.CHST1a 90/75 Cse.5.CHST1a 78/80 Sco.4.CHST1a 100/100 Hco.CHST1a 0/23 Ofa.CHST1a 79/40 Lcl.CHST1a 0/16 Bba.CHST1a 74/66 Ate.CHST1a 89/99 Mar.CHST1a 0/12 Gac.II.CHST1a 83/29 Lcr.VIII.CHST1a 0/18 Sma.5.CHST1a 69/39 Hru.CHST1a 80/100 Mjc.CHST1a 0/31 Mmo.CHST1a 100/100 Tni.5.CHST1a 0/16 96/100 Tru.13.CHST1a Sml.CHST1a 78/86 Tal.CHST1a Mpe.CHST1a 78/70 Sca.CHST1a 83/45 Squ.CHST1a 80/92 Mja.CHST1a 77/54 0/27 Sni.CHST1a 87/97 Ola.3.CHST1a 100/100 Nfu.7.CHST1a 82/100 Xma.4.CHST1a 0/31 93/100 Pre.3.CHST1a Pfu.CHST1a 0/70 Ape.1.CHST1a 90/97 Aci.CHST1a 81/90 Oni.1.CHST1a 88/100 Sdi.17.CHST1a 89/100 Asc.1.CHST1a 0/65 Mze.1.CHST1a

0.4 Ebu.CHST16 52/90 Pma.CHST16 99/100 Lca.CHST16 Pbu.CHST16 Cmi.CHST16 89/96 Sto.CHST16* 100/100 52/90 Rty.CHST16 Lch.CHST16 Ame.CHST16 86/98 92/100 Nvi.CHST16 99/100 Npa.CHST16 100/100 Xtr.3.chst16 100/100 Xla.3L.chst16 92/95 Loc.8.CHST16 Pki.CHST16 96/100 Agi.CHST16 100/100 Sfo.CHST16 97/100 Obi.CHST16 100/100 Cha.CHST16 100/100 Spi.CHST16 100/100 Aal.CHST16 Amx.18.CHST16 94/99 85/100 100/100 Pna.CHST16 97/100 Eel.CHST16 95/99 Ipu.4.CHST16 97/99 Dre.18.CHST16 92/100 Ddr.CHST16 100/100 Lwa.6.CHST16 69/100 Cau.18.CHST16.1 99/100 94/100 Cau.43.CHST16.2 Elu.19.CHST16 98/100 Sal.4.CHST16.1 100/100 Ssa.10.CHST16.1 77/99 Omy.2.CHST16.1 77/97 Omy.1.CHST16.2 98/100 Ssa.16.CHST16.2 94/98 75/100 Sal.26.CHST16.2 Oep.CHST16* Gmo.CHST16 79/99 4/67 Rgl.CHST16* 100/100 Lgu.CHST16 97/99 Mja.CHST16 Mjc.CHST16 29/100 90/99 Hru.CHST16 29/88 Bba.CHST16 89/98 Hco.CHST16 100/100 Sco.2.CHST16 77/51 Tal.CHST16 71/73 Mar.CHST16 96/99 Lcr.XXI.CHST16 Ate.CHST16 0/33 Lcl.CHST16 74/44 Squ.CHST16 83/52 Dla.6.CHST16 0/44 Mmo.CHST16 96/89 Tni.13.CHST16 100/100 Tru.9.CHST16 52/54 Mpe.CHST16 0/28 Nfu.13.CHST16 98/98 Xma.2.CHST16 100/100 Pre.6.CHST16 72/42 Sca.CHST16* 18/74 Ofa.CHST16 70/70 Gac.XIX.CHST16 41/87 Sni.CHST16 3/60 Pfv.CHST16 61/39 Sma.10.CHST16 73/74 Cse.CHST16 73/39 Ola.6.CHST16 65/87 Sml.CHST16 88/77 Bpe.CHST16 100/100 87/42 Pmg.CHST16 Ape.4.CHST16 82/56 Pfu.CHST16 10/35 Aci.CHST16 100/100 Oni.7.CHST16 93/98 Sdi.22.CHST16 40/85 Mze.7.CHST16 0/82 Asc.7.CHST16

0.3 Lca.CHST3 100/100 Pma.CHST3* Cmi.CHST3 Rty.CHST3 98/100 Sto.CHST3 99/100 Ara.CHST3 100/100 Oke.CHST3 Lch.CHST3 Rbi.CHST3 91/99 Ame.CHST3 100/100 100/100 Nvi.CHST3 7/76 Npa.CHST3 100/100 Xtr.7.chst3 99/100 Xla.7L.chst3.L 78/99 Xla.7S.chst3.S 94/99 Psi.CHST3 99/100 Cmy.CHST3 42/94 89/99 Cpi.CHST3 85/80 Ami.CHST3 100/100 Cpo.CHST3 0/27 Spp.CHST3 92/96 Ppi.CHST3 99/100 Pvi.CHST3 42/88 Aca.3.CHST3 65/88 Lvi.CHST3 91/88 Pte.CHST3 100/100 Tsi.CHST3* 64/90 Pfl.CHST3 94/91 Pbi.CHST3 99/100 100/98 Oan.CHST3 100/100 Pci.CHST3 98/100 Mdo.Un.CHST3 92/100 Cfa.4.CHST3 100/100 Ocu.18.CHST3 84/93 Dno.CHST3* 76/73 Dro.CHST3* 19/52 Rfe.CHST3 36/61 Ssc.14.CHST3 100/100 Pca.20.CHST3 76/61 Laf.CHST3 1/25 Mmu.10.Chst3 90/72 88/99 Ccr.CHST3 90/68 Cja.12.CHST3 99/100 Sbo.CHST3 98/98 Mml.9.CHST3 88/100 Ptr.10.CHST3 96/100 Hsa.10.CHST3 Ram.CHST3 Abr.CHST3 86/100 Acy.CHST3 100/100 Apl.6.CHST3 19/73 89/98 Nme.5.CHST3 100/100 100/100 Tcu.CHST3 93/100 Gga.6.CHST3 88/100 Mga.8.CHST3 Sha.CHST3 99/100 100/100 Mun.CHST3 0/68 Can.6.CHST3 88/85 Ccn.CHST3* 83/92 Nha.CHST3 0/85 Cli.CHST3* 99/100 Pfa.CHST3 100/100 Lco.CHST3 100/100 Pdo.6.CHST3 37/97 Tgu.6.CHST3 99/100 Fal.6.CHST3 42/94 Pmj.6.CHST3 48/74 Afo.CHST3 95/100 Pan.CHST3* 76/100 Sme.CHST3* 73/63 Soc.CHST3 0/57 Ach.CHST3 97/99 Ppu.CHST3 86/77 Ulo.CHST3 80/96 Fpe.CHST3 93/100 Fch.CHST3* Loc.5.CHST3 Acl.CHST3 Aja.CHST3b 100/100 Aan.CHST3b Aja.CHST3a 100/100 Aan.CHST3a 100/100 Dre.13.chst3a 90/100 Ddr.CHST3a 100/100 Lwa.8.CHST3a 59/99 Cau.CHST3a*3of3( ) 29/99 90/87 Cau.13.CHST3a*1of3( ) 98/100 100/100 Cau.CHST3a*2of3( ) 88/97 Cha.CHST3a 100/100 Spi.CHST3a 83/100 Ipu.3.CHST3a 100/100 Eel.CHST3a 100/100 86/100 Amx.25.CHST3a 41/99 Pna.CHST3a Oep.CHST3a Lgu.CHST3a 100/100 Rgl.CHST3a 100/100 Mja.CHST3a 100/100 75/77 Hru.CHST3a 100/100 100/100 Mjc.CHST3a* Gmo.CHST3a Bba.CHST3a 86/82 24/69 Bpe.CHST3a 10/100 Pmg.CHST3a 26/55 Sco.22.CHST3a 100/100 Hco.CHST3a 37/69 86/97 Cse.12.CHST3a.1* 100/100 Cse.12.CHST3a.2* 100/100 Cse.12.CHST3a.3 Mpe.CHST3a 83/61 Tal.CHST3a 77/46 81/84 Ofa.CHST3a 84/84 Sml.CHST3a 80/83 Sni.CHST3a 71/100 Pfv.CHST3a 63/97 Gac.VI.CHST3a Aci.CHST3a* 46/82 100/100 Mze.13.CHST3a 77/100 93/69 Asc.13.CHST3a 100/100 Oni.13.CHST3a 76/100 96/81 Sdi.CHST3a Ape.20.CHST3a 71/94 Pfu.CHST3a 70/74 Ola.15.CHST3a 91/100 Nfu.3.CHST3a 99/100 71/31 Xma.22.CHST3a 100/100 Pre.15.CHST3a Lcl.CHST3a 74/84 Sma.15.CHST3a 84/36 Mar.CHST3a 93/94 Ate.CHST3a 76/33 Squ.CHST3a 72/38 Sca.CHST3a 79/71 Lcr.III.CHST3a 85/59 Dla.11.CHST3a 66/77 Mmo.CHST3a 95/97 Tni.17.CHST3a 100/100 Tru.4.CHST3a Pbu.CHST3b 96/100 Agi.CHST3b 96/100 Sfo.CHST3b 83/99 Obi.CHST3b 74/94 Pki.CHST3b.2 100/100 Gpe.CHST3b 64/100 Pki.CHST3b.1 Cha.CHST3b 100/100 Aal.CHST3b 91/100 87/99 Spi.CHST3b Dre.12.chst3b 95/99 100/100 Cau.12.CHST3b 61/99 Lwa.19.CHST3b*1of2( ) 100/100 95/99 Lwa.CHST3b*2of2( ) Amx.Un.CHST3b 100/100 Pna.CHST3b 88/100 Eel.CHST3b 96/100 90/100 Ipu.13.CHST3b Elu.6.CHST3b 99/100 Sal.Un.CHST3b 86/96 Ssa.1.CHST3b 80/96 Omy.CHST3b 97/99 Oep.CHST3b Lgu.CHST3b 99/100 Rgl.CHST3b 29/90 Mja.CHST3b Mjc.CHST3b 98/100 97/100 Hru.CHST3b Sml.CHST3b 85/98 89/81 Bpe.CHST3b 100/100 Pmg.CHST3b 71/70 Bba.CHST3b 84/98 82/87 Hco.CHST3b* 100/100 Sco.10.CHST3b 73/97 Gmo.CHST3b 36/90 Gac.V.CHST3b Mpe.CHST3b 94/94 99/96 Sca.CHST3b 82/93 Lcr.XVI.CHST3b 40/89 Dla.1B.CHST3b 92/98 Mmo.CHST3b 94/99 Tni.Un.CHST3b 100/100 Tru.1.CHST3b 85/77 Tal.CHST3b 73/94 Ofa.CHST3b 88/85 Sni.CHST3b 94/95 Pfv.CHST3b 0/46 Lcl.CHST3b 80/57 Mar.CHST3b 79/73 Aci.CHST3b 100/100 Oni.8-24.CHST3b 99/100 Sdi.29.CHST3b 99/100 73/72 Mze.8.CHST3b 74/100 Asc.8.CHST3b Squ.CHST3b 75/87 Sma.18.CHST3b 83/95 Cse.8.CHST3b 77/58 Ate.CHST3b 79/68 Ape.19.CHST3b 83/81 Pfu.CHST3b 89/82 Ola.19.CHST3b 91/99 Nfu.12.CHST3b 98/100 Xma.10.CHST3b 100/100 Pre.19.CHST3b

0.5 Cmi.CHST2

100/100 Sto.CHST2 91/100 Rty.CHST2 93/100 Ler.CHST2 100/100 Oke.CHST2 33/94 Ara.CHST2 Rbi.CHST2 88/97 Ame.CHST2 100/100 Nvi.CHST2* 96/98 Npa.CHST2 89/100 Xla.5L.chst2.L 99/100 Xtr.5.chst2 61/99 Xla.5S.chst2.S Ppi.CHST2 98/99 Aca.3.CHST2 99/100 90/89 Pvi.CHST2 40/71 Lvi.CHST2 85/94 Pbi.CHST2 100/100 Pfl.CHST2 95/100 Pte.CHST2 43/100 100/100 Tsi.CHST2 Psi.CHST2 98/100 Cmy.CHST2 82/100 Cpi.CHST2 Spp.CHST2 Oan.CHST2 91/98 100/100 Pci.CHST2 89/100 Mdo.7.CHST2 74/100 Dno.CHST2 99/100 Laf.CHST2 75/98 78/90 Ccr.CHST2 * 74/97 Dro.CHST2 97/99 Rfe.CHST2 83/55 Cfa.23.CHST2 0/51 Pca.1.CHST2 89/95 Ssc.13.CHST2 98/100 76/86 Mmu.9.Chst2 61/66 Ocu.14.CHST2 21/52 Sbo.CHST2 97/100 Mml.2.CHST2 74/100 Hsa.3.CHST2 87/100 Ptr.3.CHST2 Cpo.CHST2* 100/100 Ami.CHST2* Ram.CHST2* 8.8/41 Gga.9.CHST2 99/100 100/100 Tcu.CHST2 75/95 98/100 Mga.11.CHST2* 0/88 Nme.4.CHST2* Fpe.CHST2* 87/100 90/93 Fch.CHST2* 0/37 Ppu.CHST2* 75/43 Can.9.CHST2 80/42 Oho.CHST2* 0/81 Nha.CHST2 88/40 Ach.CHST2 0/55 Ulo.CHST2 0/63 Sha.CHST2 97/100 Mun.CHST2* 71/82 Fal.9.CHST2* 0/79 Lco.CHST2 0/57 Cco.CHST2* 78/80 74/41 Tgu.9.CHST2 0/79 Pdo.9.CHST2* 38/80 Pmj.9.CHST2 Soc.CHST2 Ccn.CHST2* 58/78 71/45 Pfa.CHST2* 90/100 Cli.CHST2* 71/52 Afo.CHST2* 0/66 Pan.CHST2* 84/99 Sme.CHST2* 0/45 Acy.CHST2* 0/83 Apl.6.CHST2 0/92 Abr.CHST2 Lch.CHST2 Pki.CHST2b 100/100 Gpe.CHST2b

84/99 Pbu.CHST2b Pki.CHST2a 98/100 Gpe.CHST2a 96/100 Agi.CHST2a 100/100 97/99 Obi.CHST2a 48/71 Sfo.CHST2a 0/81 Loc.14.CHST2 95/99 Acl.CHST2

86/81 Aja.CHST2a 33/92 100/100 Aan.CHST2a Cha.CHST2a

84/99 Spi.CHST2a Aal.CHST2a Amx.16.CHST2a 100/100 100/100 Pna.CHST2a 90/100 Eel.CHST2a 80/100 Ipu.7.CHST2a 67/90 99/100 Dre.2.chst2a 83/95 Ddr.CHST2a 100/100 Lwa.20.CHST2a 20/92 Cau.2.CHST2a.1 89/98 Cau.27.CHST2a.2 Agi.CHST2b 100/100 Sfo.CHST2b 92/99 Obi.CHST2b Pbu.CHST2a 74/74 Aja.CHST2b 99/100 Aan.CHST2b 27/59 Gmo.CHST2b 73/60 Cha.CHST2b 93/100 Aal.CHST2b 17/27 Spi.CHST2b 74/51 Cau.24.CHST2b.1 74/75 100/100 Cau.49.CHST2b.2 73/100 Lwa.11.CHST2b 17/94 98/100 Dre.24.chst2b Eel.CHST2b 99/100 Ipu.1.CHST2b 85/100 Amx.Un.CHST2b 99/100 91/100 Pna.CHST2b Sal.14CHST2b.1 91/61 Ssa.19.CHST2b.1 0/28 Omy.11.CHST2b.1 95/100 Elu.21.CHST2b 0/29 Omy.15.CHST2b.2 100/99 Ssa.29.CHST2b.2 30/54 95/71 Sal.Un.CHST2b.2 Oep.CHST2b Hru.CHST2b 74/99 86/54 Mjc.CHST2b Mja.CHST2b 95/66 Sml.CHST2b Mpe.CHST2b 77/43 79/51 Mmo.CHST2b* 85/86 Tni.6.CHST2b* 42/72 Tru.6.CHST2b 86/50 74/46 Nfu.8.CHST2b 78/68 Xma.21.CHSR2b 92/100 Pre.20.CHST2b 39/33 Aci.CHST2b 89/100 76/42 Oni.9.CHST2b 92/100 Asc.9.CHST2b 77/100 Mze.9.CHST2b 0/0 Sdi.12.CHST2b Lcr.XXIII.CHST2b 0/8 Bba.CHST2b 0/0 Lcl.CHST2b 0/2 Ofa.CHST2b 0/1 Pfu.CHST2b 0/2 Tal.CHST2b 0/4 Sma.21.CHST2b 84/42 Squ.CHST2b 0/22 Dla.18-21.CHST2b 0/19 Sni.CHST2b 73/64 Pfv.CHST2b 0/31 Gac.XXI.CHST2b 39/39 Lgu.CHST2b 86/97 Rgl.CHST2b 0/10 Ape.22.CHST2b 71/24 Sca.CHST2b 78/40 Cse.3.CHST2b 0/25 Bpe.CHST2b 100/100 Pmg.CHST2b 0/31 Mar.CHST2b 62/24 Ate.CHST2b 84/47 Ola.20.CHST2b 77/46 Hco.CHST2b* 100/100 Sco.21.CHST2b

0.2 Cmi.CHST7 Rty.CHST7 100/100 95/100 Sto.CHST7 90/97 Oke.CHST7 100/100 Ara.CHST7 91/100 Ler.CHST7 Lch.CHST7 Ame.CHST7 100/100 Nvi.CHST7 92/99 Npa.CHST7 100/100 Xtr.2.chst7 98/100 Xla.2L.chst7.L 89/100 Xla.2S.chst7.S Pci.CHST7 100/100 Mdo.7.CHST7

97/99 Laf.CHST7 Cfa.X.CHST7 57/52 Ccr.CHST7 100/100 90/56 Dno.CHST7 86/33 Dro.CHST7 75/44 Mmu.X.Chst7 86/42 Ocu.X.CHST7 37/43 Rfe.CHST7 88/71 100/100 Pca.21.CHST7 85/100 Ssc.X.CHST7 87/97 77/43 Sbo.CHST7 98/100 Cja.X.CHST7* 93/100 Mml.X.CHST7 87/100 Hsa.X.CHST7 97/100 Ptr.X.CHST7 Aca.3.CHST7 87/97 Ami.CHST7 100/100 Cpo.CHST7 Ppi.CHST7 92/99 Lvi.CHST7 100/99 96/100 Pbi.CHST7* 100/100 Pfl.CHST7 79/96 Pte.CHST7 70/95 65/95 Tsi.CHST7 Spp.CHST7 88/100 Ram.CHST7* Gga.1.CHST7 72/91 96/100 Nme.1.CHST7 93/100 Tcu.CHST7 99/100 99/100 Apl.1.CHST7 100/100 Acy.CHST7 91/100 90/97 Abr.CHST7 Nha.CHST7 Ach.CHST7 81/77 31/84 Soc.CHST7 Can.1.CHST7 45/83 88/82 Ulo.CHST7 Pfa.CHST7 76/64 Sha.CHST7 100/100 0/75 Mun.CHST7*

85/90 Lco.CHST7 Pdo.1.CHST7 96/100 90/100 Tgu.1.CHST7 98/100 Fal.1.CHST7 * 0/98 Pmj.1.CHST7 Sfo.CHST7_2of2( ) Pbu.CHST7 73/93 Agi.CHST7 92/99 98/100 Obi.CHST7 93/100 Sfo.CHST7_1of2*( )* 93/96 Amx.13.CHST7 100/100 Pna.CHST7 Ipu.11.CHST7 15/64 Cau.6.CHST7.1 100/100 Dre.6.chst7 86/88 38/75 Cau.31.CHST7.2 75/87 Ddr.CHST7 83/99 Lwa.4.CHST7 95/82 Eel.CHST7 Cha.CHST7 99/100 Spi.CHST7 87/100 59/70 Aal.CHST7 Pki.CHST7 100/100 Gpe.CHST7 78/88 84/79 Loc.17.CHST7 97/99 Acl.CHST7 72/89 Aja.CHST7 100/100 Aan.CHST7 87/80 Elu.8.CHST7 98/100 Ssa.CHST7 100/100 Omy.1.CHST7 44/99 100/93 Sal.Un.CHST7 Oep.CHST7 Lgu.CHST7 100/100 98/93 Rgl.CHST7 Gmo.CHST7 92/91 Mja.CHST7 19/80 Mjc.CHST7 77/92 90/86 Hru.CHST7 Bba.CHST7 92/83 Tal.CHST7 Pfv.CHST7 73/84 98/91 Mpe.CHST7 75/68 Squ.CHST7 84/74 Mar.CHST7 83/76 99/87 Ate.CHST7 92/88 Aci.CHST7 Lcl.CHST7 77/77 Ape.2.CHST7 91/98 Pfu.CHST7 34/63 15/55 Sma.12.CHST7 80/82 Ola.4.CHST7 83/85 Nfu.CHST7 83/99 Xma.9.CHST7 100/100 Pre.4.CHST7 Lcr.XVII.CHST7 89/94 53/59 Ofa.CHST7 74/58 Sml.CHST7 81/50 Dla.4.CHST7 92/80 Mmo.CHST7 76/64 Sni.CHST7 72/73 Sca.CHST7* 78/48 Tni.1.CHST7 100/100 74/63 Tru.20.CHST7 Hco.CHST7 100/100 Sco.9.CHST7 86/61 Cse.2.CHST7 20/62 Bpe.CHST7 100/100 90/65 Pmg.CHST7 Gac.CHST7 90/58 Oni.23.CHST7 100/100 Mze.23.CHST7 71/90 Asc.23.CHST7 0/53 Sdi.5.CHST7

0.4 1 6 0 5 9 4 2 3 3 4 4 4 7 2 6 35. 35. 35. 35. 35. 35. 36. 37. 1 2 . . a a a p 3 3 a a 6 p p 1 4 5 1 8 n n st7 s i i s s s s g r r o h g g g g n r r r r b b c o b b r M f M

10

d Chr. 6 10 e w

Chr. 8 a Chr. 4 l Chr. 2 7 8 5 6 8 1 2 h c 4 3 4 0 7 3

9 0 2 9 9 1 6 8 3 9 3 1 ...... s 5 8 0 8 8 9 b . 4 7 8 9 1 3 4 4 5 5 7 8 i n . . . . 6 6 2 4 1 9 8 f M r 6 6 6 8 88. 90. 97. 97. 97. 6 3 4 4 5 5 1 2 3 4 5 8 6 P 34. a 106 1 e P P S S S A a 10 b b b r S 19. 20. 20. 20. 20. 20. 28. 1 2 N N G G G 5 3 cken p 6 4 5 2 3 p i s p G b S a s s p R R R 1 a p p st2 a n g 1 s n R g g 1 i O n n i r a e est h h i i r r BRI BRI P r s r g s r r 3 N c r 7 o b A 6 a o b Z b b C P n T 1 8 1 5 4 n W N S S S S S O G G G G CHS BRI R R R R N b b M M 10 10 Chr. 4

Chr. 1 LG10

Chr. 17 b r 5 2 5 2 9 3 5 3 1 M 7 1 3 7 0 5 8 a 9 0 0 2 5 0 3 5 1 6 8 6 4 . . 0 0 3 1 6 7 4 ...... 6 6 7 3 1 1 g 10 10. 11. 11. 11. 11. 21.

16. 16. 16. 20. 162 163 163 177 182 182 190 d n 2 3 4 1 2 2 1 4 2 P b b b ...... 3 2 8 6 5 4 P P S a 3 5 P A P 3 3 3 3 5 5 1 P P S S S aka A 1 P S N G N A P P P P S S S 1 N N G G G S d 1 N R m G N N N N G G G S R R R S O R R R e R BRI O u BRI potte BRI BRI O N BRI N BRI BRI BRI BRI N H S M Spp.CHST4/5-like 100/100 Ppi.CHST4/5-like 97/100 Lvi.CHST4/5-like 86/98 Aca.2.CHST4/5-like 38/72 100/100 Pvi.CHST4/5-like Rbi.CHST4/5-like_5of5( ) Rbi.CHST4/5-like_4of5( ) 95/68 Ame.CHST4/5_3of3( ) 92/76 100/100 Nvi.CHST4/5_2of2( ) 63/66 Ame.CHST4/5_1of3( ) Rbi.CHST4/5-like_3of5( ) 98/92 93/73 Ame.CHST4/5_2of3( ) 100/100 Nvi.CHST4/5_1of2( ) 67/66 Rbi.CHST4/5-like_1of5( ) 8/47 Rbi.CHST4/5-like_2of5( ) 97/86 Npa.CHST4/5-like_4of4( ) Xtr.8.chst4/5-like 100/100 100/100 Xla.8S.chst4/5-like 83/99 Npa.CHST4/5-like_3of4( ) 93/100 Npa.CHST4/5-like_1of4( ) 100/100 Npa.CHST4/5-like_2of4( ) Ame.CHST4 99/100 Nvi.CHST4 99/100 Npa.CHST4 96/100 Xla.4L.chst4.L 100/100 Xla.4S.chst4.S Ram.CHST4 Apl.11.CHST4 100/100 Acy.CHST4 99/100 Abr.CHST4 92/100 Nme.10.CHST4 100/100 100/100 Gga.11.CHST4 97/100 Tcu.CHST4 96/100 Mga.13.CHST4

98/100 Nha.CHST4 77/96 86/88 Soc.CHST4 0/58 Fpe.CHST4 100/100 Fch.CHST4 84/64 Afo.CHST4 98/100 Sme.CHST4 74/100 Pan.CHST4 96/87 Can.11.CHST4 81/69 Ach.CHST4 Ppu.CHST4 90/97 Ccn.CHST4 69/97 Ulo.CHST4 75/87 0/46 97/100 Oho.CHST4 37/89 Mun.CHST4 100/100 Sha.CHST4 76/59 Lco.CHST4 76/97 Cli.11.CHST4 100/100 Pfa.CHST4 94/96 Pdo.11.CHST4 0/99 Tgu.11.CHST4 94/97 Cco.CHST4 73/96 Fal.11.CHST4 0/93 Pmj.11.CHST4 Psi.CHST4 100/100 Cmy.CHST4 95/100 Cpi.CHST4_1of2( ) 54/100 Cpi.CHST4_2of2( ) 88/21 Ami.CHST4 100/100 Cpo.CHST4 Spp.CHST4 Ppi.CHST4 71/19 92/99 Lvi.CHST4 50/26 Pvi.CHST4 91/97 Aca.CHST4 31/21 76/91 Pbi.CHST4 100/100 Pte.CHST4 97/100 Pfl.CHST4 89/24 87/100 Tsi.CHST4 Oan.CHST4.1-1 100/100 Oan.CHST4.2-2 Mdo.1.CHST4.1-1 100/100 Mdo.1.CHST4.2-2 100/100 100/100 Pci.CHST4.1-1 99/100 Pci.CHST4.2-2 85/100 96/100 Pci.CHST4.3-3 Dno.CHST4 Laf.CHST4 100/100 Mmu.8.Chst4 91/90 Ocu.5.CHST4 92/99 Sbo.CHST4 98/100 Cja.20.CHST4 100/99 100/100 Mml.20.CHST4 94/100 100/100 Hsa.16.CHST4 92/100 19/85 Ptr.16.CHST4 Ccr.CHST4 97/100 Pca.17.CHST4 100/100 Ssc.6.CHST4 75/56 Cfa.5.CHST4 83/56 Dro.CHST4 95/100 Rfe.CHST4 Ame.CHST5 100/100 Nvi.CHST5 Rbi.CHST5.1.1 100/100 Rbi.CHST5.1.22 Ami.CHST5 99/100 Cpo.CHST5 81/98 Ram.CHST5 87/89 100/100 Apl.11.CHST5 94/100 Acy.CHST5 85/100 Abr.CHST5 75/99 Nme.10.CHST5 99/100 Mga.13.CHST5 0/86 Tcu.CHST5 0/76 80/87 Gga.11.CHST5 74/98 Soc.CHST5 90/89 Mun.CHST5 98/100 Sha.CHST5 97/100 Ulo.CHST5 0/68 Sme.CHST5 96/100 Afo.CHST5 0/92 Pan.CHST5 0/6 Ppu.CHST5 85/82 Pfa.CHST5 88/100 Cli.11.CHST5 78/14 Can.11.CHST5 0/22 Oho.CHST5 74/87 Ccn.CHST5 0/16 Nha.CHST5 0/6 Ach.CHST5 75/31 Fpe.CHST5 90/100 Fch.CHST5 75/96 Lco.CHST5 94/99 Cco.CHST5 92/97 Pmj.11.CHST5 0/79 Tgu.11.CHST5 72/87 Fal.11.CHST5 77/87 Pdo.11.CHST5 Psi.CHST5 97/100 Cmy.CHST5 88/100 Cpi.CHST5 Spp.CHST5 74/84 Lvi.CHST5 49/40 Pvi.CHST5 81/42 Ppi.CHST5 94/93 48/80 Pbi.CHST5_2of2( ) 97/87 100/100 Pbi.CHST5_1of2( ) 97/100 Pfl.CHST5 100/100 98/99 Pte.CHST5 79/100 Tsi.CHST5 Aca.CHST5 Mdo.1.CHST5 97/97 100/100 Pci.CHST5 Oan.CHST5.1 100/100 100/100 Oan.CHST5.2

96/100 Dno.CHST5 Mmu.8.Chst5 66/81 Laf.CHST5 100/100 83/36 Ssc.6.CHST5 98/97 Pca.17.CHST5 66/64 Ccr.CHST5 0/59 Cfa.5.CHST5 61/94 Rfe.CHST5 61/96 Dro.CHST5 59/33 Ocu.5.CHST5 Mml.20.CHST6 89/99 Hsa.16.CHST6 85/90 94/100 Ptr.16.CHST6 Sbo.CHST6 100/100 90/99 Cja.20.CHST6 66/98 Sbo.CHST5 100/100 Cja.20.CHST5 100/100 Mml.20.CHST5 84/100 Hsa.16.CHST5 100/100 Ptr.16.CHST5 Npa.CHST5.1 99/89 Xla.4S.chst5.1.S 100/100 Xtr.4.chst5.1 75/95 Xla.4L.chst5.1.L 34/84 Npa.CHST5.2.1 96/99 Npa.CHST5.2.2 93/87 Xtr.4.chst5.2 100/100 41/71 Xla.4L.chst5.2.L 94/100 Xla.4S.chst5.2.S Lch.CHST5 Cmi.CHST5 100/100 Rty.CHST5 95/100 Sto.CHST5 91/100 97/100 Oke.CHST5 100/100 Ara.CHST5 93/100 40/86 Ler.CHST5 Loc.23.CHST5 Acl.CHST5 Gpe.CHST5 100/100 100/100 Pki.CHST5_2of2( ) 67/97 Agi.CHST5_2of2( ) 99/100 Sfo.CHST5_2of2( ) 43/97 98/100 Obi.CHST5_2of2( ) Aan.CHST5 100/100 Aja.CHST5 89/100 Pki.CHST5_1of2( ) 100/100 Pbu.CHST5 100/100 73/100 Agi.CHST5_1of2( ) 95/100 Sfo.CHST5_1of2( ) 97/100 Obi.CHST5_1of2( ) Cha.CHST5 100/100 Spi.CHST5_1of2( ) 100/100 Aal.CHST5 82/98 27/92 Spi.CHST5_2of2( ) 96/99 Eel.CHST5 99/100 Amx.Un.CHST5 93/100 Pna.CHST5 82/100 Ipu.8.CHST5

61/99 Lwa.10.CHST5 100/100 Ddr.CHST5 100/100 Dre.25.chst5 93/99 26/88 Cau.25.CHST5.1 97/98 Cau.50.CHST5.2 Oep.CHST5 48/94 Elu.LG19.CHST5 95/99 Omy.1.CHST5 96/99 Ssa.16.CHST5 82/99 Sal.26.CHST5 97/100 Gmo.CHST5 Lgu.CHST5 87/100 99/100 Rgl.CHST5 Mja.CHST5* 57/59 Mjc.CHST5 100/100 Hru.CHST5 Mmo.CHST5 89/62 100/100 Tni.CHST5 100/100 Tru.9.CHST5

16/59 Ofa.CHST5 98/100 Mpe.CHST5 Ape.4.CHST5 89/83 Aci.CHST5 55/98 Oni.7.CHST5.1 100/100 5/62 Sdi.CHST5.1 99/100 Mze.7.CHST5.1 96/100 Asc.7.CHST5.1 100/100 Oni.7.CHST5.2 100/100 Oni.7.CHST5.3 97/98 Oni.7.CHST5.4 83/87 75/83 79/96 Sdi.CHST5.2 100/100 Mze.7.CHST5.2 97/100 Asc.7.CHST5.2 Ola.CHST5 100/98 Xma.2.CHST5.2 100/100 85/100 Pre.6.CHST5.2 Nfu.13.CHST5.1 96/98 Nfu.13.CHST5.4 96/100 Nfu.13.CHST5.2 99/100 47/74 Nfu.13.CHST5.6 100/100 87/60 Nfu.13.CHST5.7 Xma.2.CHST5.1 100/100 Pre.6.CHST5.1 54/59 Xma.2.CHST5.3 100/100 Pre.6.CHST5.3 97/99 Xma.2.CHST5.4 100/100 Pre.6.CHST5.4 Lcl.CHST5 73/64 Sml.CHST5.2 97/93 Pfu.CHST5_1of3( ) 88/76 Sml.CHST5.1 32/36 Dla.6.CHST5 38/51 Lcr.XXI.CHST5 82/70 Bba.CHST5 90/61 Gac.CHST5 20/86 Sca.CHST5 18/58 Sma.10.CHST5 87/68 Squ.CHST5 95/98 Cse.6.CHST5.1 100/100 72/29 Cse.6.CHST5.2 Sni.CHST5 90/29 Pfv.CHST5 75/26 Sco.CHST5 100/100 Hco.CHST5 88/72 Bpe.CHST5 100/100 Pmg.CHST5 94/86 Tal.CHST5 82/71 Mar.CHST5 82/74 Ate.CHST5.1 100/100 Ate.CHST5.3 100/100 Ate.CHST5.15 22/60 Ate.CHST5.10 95/83 Ate.CHST5.12 92/97 Ate.CHST5.6 84/69 Ate.CHST5.5 33/57 Ate.CHST5.4 90/88 Ate.CHST5.14 96/92 Ate.CHST5.7 78/90 Ate.CHST5.8 79/90 Ate.CHST5.2 88/96 Ate.CHST5.9

0.4 Anole lizard 2 Chr. Human

SETD5 GABBR1 Chr. 3 Chr. LHFPL4 ZNRD1

MTMR14 RPP21 5 Chr.

FAM3D CPNE9 GNL1 6 Chr. FAM107A BRPF1 ABCF1 ACOX2 OGG1 PPP1R10 KCTD6 10 Mb CAMK1 MRPS18B PDHB TADA3 ATAT1 PXK ARPC4 C6orf136 (RPP14) RPUSD3 DHX16 ZBED8 RPP14 JAGN1 NRM MGAT1 PRKAR2A IL17RE GTF2H4 TRIM7 SLC25A20 GTF2H4 IL17RC VARS2 GNB2L1 ARIH2 VARS2 CRELD1 DDX39B P4HTM NRM PRRT3 PRRC2A WDR6 GNL1 HDAC11 BAG6 DALRD3 ABCF1 PLXNB1 APOM NDUFAF3 MRPS18B CCDC51 CSNK2B PRKAR2A IMPDH2 180 Mb ATAT1 NEU1 QRICH1 C6orf136 SLC25A20 SLC44A4 QARS DHX16 ARIH2 EHMT2 USP19 RPP21 P4HTM ZBTB12 LAMB2 ZNRD1 WDR6 NELFE CCDC71 MGAT1 DALRD3 SKIV2L GNB2L1 CSNK2B NDUFAF3 DXO TRIM7 PRRC2A IMPDH2 STK19 CHST4/5-like PANK3 BAG6 QRICH1 ATF6B HDAC11 APOM QARS PRRT1 CCDC105 DDX39B USP19 PLXNB1 NEU1 LAMB2 SETD5 STK19 CCDC71 LHFPL4 DXO RPP14 MTMR14 SKIV2L PXK CHST2 CPNE9 NELFE PDHB BRPF1 ZBTB12 KCTD6 OGG1 EHMT2 ACOX2 CAMK1 SLC44A4 FAM107A TADA3 PRRT1 FAM3D ARPC4 ATF6B RPUSD3 ZBED8 JAGN1 IL17RE 10 Mb IL17RC CRELD1 PRRT3 PRRT10

Spotted gar LG14 LG13 USP19 LG17 LG22 LG29 LAMB2 LG5 LG8 LG9 LG6 KCTD6B PDHB PXK PRRT1 RPP14 ZBTB12 CCDC51 RPP21 PLXNB1B CAMK1D DDX39A STK19 NEU1 SETD5 DXO GNB2L1 LHFPL4 JAGN1 MTMR14 CPNE9 HDAC11 P4HTM 10 Mb WDR6 DALRD3 NDUFAF3 IMPDH2 ARIH2 SLC25A20 PRKAR2AB QRICH1 QARS OGG1 BRPF1 ARPC4 TADA3L Xenopus laevis Xenopus tropicalis Parker’s slow frog

Chr 4L Chr 4 scaffold216 scaffold195 42.22 48.68 53.02 54.89 66.63 1.41 1.56 1.30 1.32

Chr 4S Chr 8 scaffold731 32.51 40.59 44.49 1.22 0.49 0.53 0.56 0.59

Chr 8S 1.22 Axolotl Two-lined caecilian

scaffold_676 3.04 5.98 10.88 10.88

scaffold_699 3.00 4.44 1.70 15.66 15.73 15.76 15.84 15.85 Anole lizard Tuatara

chrUn0291 ScrUdWx_67 23.75 28.29 0.025 0.17 chst4 chst5.2 Chr 2 ScrUdWx_1868 chst5 chst5.1 ”CHST4/5-like” 192.75 1.22