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Primary Antibodies & Antigens

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Primary Antibodies & Antigens Introduction Some History... The use of primary antibodies (I Abs) as research tools is, in many ways, responsible One of the earliest use for antibodies, came in the for much of the progress seen in modern biological research. These antibodies, early part of the 20th Century when Karl specific of target antigens, are a first step to probe samples in various detections. Landsteiner developped a serological test for They can also be used to immobilize antigens on supports. Secondary antibodies (II different blood groups (ABO system for typing Abs) are used in a second step to detect and amplify I Abs signal by colour or light blood) identification. Today antibodies are used as versatile reagents for an extremely wide range emission as fluorescence or chemiluminescence. of assays, in techniques as varied as screening phage libraries, biosensors, affinity purifications, Interchim is proud to offer one of the largest choice of high quality primary antibodies and conventional immunoassays including (more than 20 000 items). ELISA, blotting, flow cytometry, agglutinations... Because of these multiple uses, a wide range of To make your search easier among this huge list, we propose two different ways of antibodies is needed (regarding to antigen- classification: specificity, binding affinity, and format/purity). Fortunately, a large number of companies now provide antibodies for researchers. This means ! Research Interests that each individual lab no longer has to go through 24 research areas are listed and shortly presented hereafter. Each area lists the hassle of generating, purifying and testing key words (specificities) that can be searched in the antibody alphabetical their own antibodies. However it is often difficult listing. A detailed description of some key related antibodies is inserted in the to find out the antibody companies, and the best alphabetical listing of antibodies. supplier. ! Alphabetical Listing This list presents our antibodies by specifity in alphanumerical order of specificities. It displays key informations : species cross reactivity, host, quantity, compatible immunotechniques and identified related research areas. Please refer to the legend for codified information. ! A third part is dedicated to last minute Antibodies. It includes Chicken IgY antibodies and mitochondrial research antibodies. ! In a fourth part, you will find an alphabetical listing of our mayor antigens and bioactive peptides (more than 4000). The research areas corresponding to these antigens are also displayed in the table with the same codification (1-24) as for antibodies. Do not hesitate to contact us if you do not find the requested specificity or if you need further details. Immunologicals This catalog cannot be exhaustive, while our web site Thanks to : is continuously updated with new antibodies. Please ! its huge database search on our web site http://www.interchim.com/ ! its excellent technical support interchim/customers/. With our e-search tool, you can ! and its ability to provide all associated products (secondary, substrate, kits…) search by name, key words with Booleans, INTERCHIM number as well as supplier catalog number, and obtain all ordering information. Interchim is your reliable supplier for primary antibodies. A.2 We can also source any Abs for you, thanks to our If the antibody you are looking for is not available on the market, keep in mind our access to large databases and experienced custom services : relationships with most of world Abs suppliers! Custom Rabbit AND Mouse polyclonal, as well as Mouse Monoclonal. Tel 33 (0)4 70 03 88 55 !! Hot line 33 (0)4 70 03 73 06 Fax 33 (0)4 70 03 82 60 Primary Antibodies & Antigens Primary antibodies by Research Interest Hereafter are described the 24 areas for antibody classification by Research Interest. After a small description, a list of our related antibodies is displayed. More information on the antibodies can be found in the Alphabetical classification table. 1 - Allergens Have a look at Chapter F, page 3 for a list of our An allergen is a substance that causes an acute defensive reaction in a person’s food allergens detection kits. immune system. Brazil nut Histamine Brazilian Scampi Histamine Receptor 1-4 Casein alpha Lobster protein Cashew nut Shrimp protein Crab Soy protein Egg 2 - Angio / Histogenesis Histogenesis is the process of tissue formation while Angiogenesis is the formation of new blood vessels from existing vasculature. These processes are implicated in tumor growth and metastasis. Activin Receptor Type II CD61 / GPIIIa Flk-1 / VEGFR-2 Raf-1 Angiogenin CD62E Flt-1 / VEGFR-1 Thomsen-Friedenreich Antigen Angiopoietin 1-4 CD105 / Endoglin Heparan Sulfate Proteoglycan Thrombospondin BAI 1-3 CD106 / VCAM-1 HIF-1a, b, 2a Thymidine Phosphorylase BMP 1-15 CD141 / Thrombomodulin HLA Antigens Transglutaminase C Immunologicals CD29 COL1A1, C-term HLTF Transglutaminase II CD31 / PECAM-1 Elastase, neutrophil Kringles Urokinase Plasminogen activator CD34 Endothelial Marker L1 Cell Adhesion Molecule VEGF CD36 GPIIIb / GPIV Factor VIII Related Antigen Microglobulin beta 2-3 CD41 Factor XIIIa Plasminogen CD41a FAK Plasminogen Activator Inhibitor 1-2 A.3 e-mail [email protected] ! Visit our website : www.interchim.com Primary Antibodies & Antigens Primary antibodies by Research Interest 3 - Apoptosis / cell cycle Apoptosis : Also known as programmed cell death, apoptosis is the process in which abnormal cells are eliminated and prevented from further cell divisions. Have a look at Chapter E for more details on Cell Cycle : Cell division. A complete cell cycle consists of the G1, S, G2 and M phases. Apoptosis and Cell Cycle and for a list of our In mitosis a single cell gives 2 identical daughter cells, while in meiosis a single cell related kits. gives raise to 4 haploid cells. 14-3-3 gamma C-Jun JunB, JunD PERP 8-oxo-dG CLAN Ki-67 PHO 1,3 A1, BH3 Domain clAP 1-2 Klotho PKA, PKB, PKC Acinus C-Myc Kos1 Plakoglobin Activating Transcription Factor 1-6 Cohesin, Scc1-subunit Livin Plakophilin 1, 2 AKT 1-3 Cullin 1-3 MADD Polymerase beta Anaphase Promoting Complex 2, 11 CXCR4 / Fusin Malondialdehyde/MDA Post Meiotic Segregation Increased 2 Annexin II, monomer Cyclic AMP MAP Kinase PPM1D / WIP1 Annexin VI, p70 Cyclic GMP MAP Kinase Phosphatase X Prohibitin Apaf-1 Cyclin A, B, C, D, E, G, T MAP1 Proliferating Cell Nuclear Ag APE / Ref-1 Cyclin cdc2, 4, 5 MAP126 Prostate Apoptosis Response-4 Apoptosis Inducing Factor Cytochrome B, C MAP2a,b Protein Kinase Type II ARC (Apoptosis repressor with Cytotoxic Eff. T Cells mBad, phospho PROX1 CARD) D4-GDI Mcl-1 Proximal sequence element 1/Ku Ask1 / MAPKKK5 DAP-3, DAP-5 MEK6 Prot. ATM DATF1 MEKK5 PUMA ATPase p97 DcR 1-3 Microphthalmia Rad9, BH3 Domain Aurora 2 (STK15), A, B, C DIABLO Miotic Kinesin-Like Protein-1 Raf-1 Aven DMC-1 Mitotic Proteins RAIDD AXUD1 DNA Monopolin RANK Bad, BH3 Domain DNA Fragmentation Factor 45 MSF Retinoblasma BAG-1 DNA Polymerase NBL1 Ribonucleoprotein Bak DP-1, DP-2 NFkB RNA Polymerase II BARD1 DR 3-6 NF-Y ROC Basic Ig-like variable motif-containing Drp-1 NIP1, BH3 Domain Separase Bax E2F-1, 3, 4, 6 NIP3, BH3 Domain SIRT2 Bcl-2, 2a, 6, 10, G, X, w, XL E2F 1-5 Transcription Factor NME6, N-term Smad 3, 4 Beclin 1 E47 Nod1 (Apaf-1 like protein/CARD4) SODD (Silencer of Death Domain) BID Endostatin Noxa, BH3 Domain Sodium Iodide Symporter (hNIS) Bik, BH3 Domain EphA3 (Hek) NTAL Son of Sevenless-1 (SOS-1) Bim FADD Nuclear Ag of proliferating cells STAT1 BIRC 1-8 FAP-1 NuMA Superoxide Dismutase Immunologicals Bmf, BH3 Domain FAS OAS 1, 3 Survivin BNIP3L, BH3 Domain FAS Ligand p/CIP/p300 TACE / ADAM17 Bok, BH3 Domain Fen-1 p14ARF TACI Bonzo / STRL33 / TYMSTR Fodrin-alpha (spectrin-alpha) p15INK4b TATA binding protein BPDE FosB p16 TdT BPDE DNA FOXO1a p16INK4a TIA-1 BRAF35 FPG p18INK4c TID-1 BRCA 1-2 Fra 2 p19ARF Topoisomerase II alpha Bromodeoxyuridine G protein p19Skp1 TR2 BTF / BCLAF1, Abvanced panel GADD 45 p21WAF1 TRADD A.4 CAS Glutamylcysteine Synthetase gamma p27 TRAF 2, 5 Caspase 1-10 Glyceraldehyde-3-PDH p27Kip1 TRAIL / Apo2L Catenin beta Glycogen Synthetase Kinase 3 beta p35nck5a TRANCE / RANKL CD95 / Fas Granzyme B p53 Transcription Factor IIB CDC2 GRIM-19 p57 Tri-Methyl Guanosine Cap CDC6 GTPase Activating Protein p63 TROY / TAJ CDC7 Phosphatase H2AX, phosphorylated p73 USF1 CDC14A Phosphatase HEC1 p95 /NBS1 USP7 CDC25a ,b, c Histones p95VAV UVssDNA CDC34 Holocytochrome c p107 Vasoactive Intestinal Peptide Receptor CDC37 HOX-11 p130 2 CDC47 Hrk, BH3 domain PAPK VDR CDh1 HSTK12 PAR 4 VEGF CDK 1-11 ICAD (DFF45) PAR polymer Vitamin D Receptor (VDR) CENP-F Iceberg PARP WISP 1, 2 C-Ets-1 I-FLICE / CASPER PBR Wnt-1 C-Fos IkB PCNA WT1 CIDE-3 IPAF or CARD12 PCTAIRE2 XIAP CIDE-A , B Jo-1 Antigen Perforin X-linked inhibitor of Apoptosis XPF Z-DNA Tel 33 (0)4 70 03 88 55 !! Hot line 33 (0)4 70 03 73 06 Fax 33 (0)4 70 03 82 60 Primary Antibodies & Antigens Primary antibodies by Research Interest 4 - Blood and serum proteins Acid Glycoprotein alpha 1 Factor X I-309 Protein Z Acrp30 Factor XI IgA Secretory Prothrombin Albumin Factor XII Kappa Rh(o)D & Rh(o)Dii Antithrombin Factor XIII Lactalbumin alpha Rheumatoid Factor Blood Group Antigen A,B,H,M,N Ferritin Lactoferrin RhoC Blood Group Associated, A,B,Le,O Fibrin / Fibrinogen Lactoglobulin beta Secretory Component Blood Group Associated,Precursor Fibrinogen Lambda Secretory IgA Bovine Serum Albumin Fibrinopeptide A Leo1 Serum Amyloid A Ceruloplasmin Fibronectin Macroglobulin alpha 2 Serum Amyloid Component Complement C1, C1q, C3-9 Folate Myoglobin Serum Amyloid P Component C-Reactive Protein Folate Binding Protein Ovalbumin SS-A (Ro) Antigen D-dimer Globulin (Z-1) Plasminogen SS-B Antigen Factor H, beta 1H Glycophorin A Platelet Factor IV TAFI Factor V Haptoglobin PLAU Thrombin Factor VII Hemoglobin PLAUR Thrombin Receptor Factor VIII Heparin Cofactor II Prealbumin Thrombospondin Factor VIII C High MW Kininogen Prostatic Acid Phosphatase Thyroxine Binding Globulin Factor VIII Related Antigen Human Serum Albumin Protein C Transferrin Factor IX Human Whole Serum Protein S Vitronectin 5 - Cancer, Hypoxia and related molecules Cancer is a group of related diseases characterized by uncontrolled cell division.
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  • Profiling Data

    Profiling Data

    Compound Name DiscoveRx Gene Symbol Entrez Gene Percent Compound Symbol Control Concentration (nM) JNK-IN-8 AAK1 AAK1 69 1000 JNK-IN-8 ABL1(E255K)-phosphorylated ABL1 100 1000 JNK-IN-8 ABL1(F317I)-nonphosphorylated ABL1 87 1000 JNK-IN-8 ABL1(F317I)-phosphorylated ABL1 100 1000 JNK-IN-8 ABL1(F317L)-nonphosphorylated ABL1 65 1000 JNK-IN-8 ABL1(F317L)-phosphorylated ABL1 61 1000 JNK-IN-8 ABL1(H396P)-nonphosphorylated ABL1 42 1000 JNK-IN-8 ABL1(H396P)-phosphorylated ABL1 60 1000 JNK-IN-8 ABL1(M351T)-phosphorylated ABL1 81 1000 JNK-IN-8 ABL1(Q252H)-nonphosphorylated ABL1 100 1000 JNK-IN-8 ABL1(Q252H)-phosphorylated ABL1 56 1000 JNK-IN-8 ABL1(T315I)-nonphosphorylated ABL1 100 1000 JNK-IN-8 ABL1(T315I)-phosphorylated ABL1 92 1000 JNK-IN-8 ABL1(Y253F)-phosphorylated ABL1 71 1000 JNK-IN-8 ABL1-nonphosphorylated ABL1 97 1000 JNK-IN-8 ABL1-phosphorylated ABL1 100 1000 JNK-IN-8 ABL2 ABL2 97 1000 JNK-IN-8 ACVR1 ACVR1 100 1000 JNK-IN-8 ACVR1B ACVR1B 88 1000 JNK-IN-8 ACVR2A ACVR2A 100 1000 JNK-IN-8 ACVR2B ACVR2B 100 1000 JNK-IN-8 ACVRL1 ACVRL1 96 1000 JNK-IN-8 ADCK3 CABC1 100 1000 JNK-IN-8 ADCK4 ADCK4 93 1000 JNK-IN-8 AKT1 AKT1 100 1000 JNK-IN-8 AKT2 AKT2 100 1000 JNK-IN-8 AKT3 AKT3 100 1000 JNK-IN-8 ALK ALK 85 1000 JNK-IN-8 AMPK-alpha1 PRKAA1 100 1000 JNK-IN-8 AMPK-alpha2 PRKAA2 84 1000 JNK-IN-8 ANKK1 ANKK1 75 1000 JNK-IN-8 ARK5 NUAK1 100 1000 JNK-IN-8 ASK1 MAP3K5 100 1000 JNK-IN-8 ASK2 MAP3K6 93 1000 JNK-IN-8 AURKA AURKA 100 1000 JNK-IN-8 AURKA AURKA 84 1000 JNK-IN-8 AURKB AURKB 83 1000 JNK-IN-8 AURKB AURKB 96 1000 JNK-IN-8 AURKC AURKC 95 1000 JNK-IN-8
  • ILC2 Activation by Protozoan Commensal Microbes

    ILC2 Activation by Protozoan Commensal Microbes

    International Journal of Molecular Sciences Review ILC2 Activation by Protozoan Commensal Microbes Kyle Burrows 1 , Louis Ngai 1 , Flora Wong 1,2, David Won 1 and Arthur Mortha 1,* 1 University of Toronto, Department of Immunology, Toronto, ON M5S 1A8, Canada; [email protected] (K.B.) [email protected] (L.N.); fl[email protected] (F.W.); [email protected] (D.W.) 2 Ranomics, Inc. Toronto, ON M5G 1X5, Canada * Correspondence: [email protected] Received: 3 September 2019; Accepted: 27 September 2019; Published: 30 September 2019 Abstract: Group 2 innate lymphoid cells (ILC2s) are a member of the ILC family and are involved in protective and pathogenic type 2 responses. Recent research has highlighted their involvement in modulating tissue and immune homeostasis during health and disease and has uncovered critical signaling circuits. While interactions of ILC2s with the bacterial microbiome are rather sparse, other microbial members of our microbiome, including helminths and protozoans, reveal new and exciting mechanisms of tissue regulation by ILC2s. Here we summarize the current field on ILC2 activation by the tissue and immune environment and highlight particularly new intriguing pathways of ILC2 regulation by protozoan commensals in the intestinal tract. Keywords: ILC2; protozoa; Trichomonas; Tritrichomonas musculis; mucosal immunity; taste receptors; succinate; intestinal immunity; type 2 immunity; commensals 1. The ILC Lineage 1.1. The Family of Innate Lymphoid Cells Research over the last decade has redirected focus away from classical immune cell interactions within lymphoid tissues towards immunity within non-lymphoid tissues. Within these tissues, immune interactions involve local adaptation and rapid responses by tissue-resident immune cells.