Efficient Professional and Antibody Platforms (TriMethyl K20) Antibody Basic information: Catalog No.: UMA21038 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal 7A2A7 Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: The antibody was purified by immunogen affinity chromatography. Useful Information: WB:1:500 - 1:2000 Applications: FCM:1:200 - 1:400 ELISA:1:10000 Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes Histone H4 (TriMethyl K20) protein. Immunogen: Synthesized peptide of human HIST2H4A (AA: GGAKRHRK(Me3)VLRDNIQ) . Histones are basic nuclear that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker Description: histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on 1. This gene is one of four histone in the cluster that are duplicated; this record represents the centromeric copy. Uniprot: P62805 BiowMW: 11.4kDa Buffer: Purified antibody in PBS with 0.05% sodium azide Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Figure 2:Western blot analysis using HIST2H4A(20Me3) mouse mAb against THP-1 (1), Jurkat (2), K562 (3), NIT/3T3 (4), PC-12 (5), Hela (6), MCF-7 (7), and A431 (8) cell lysate.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Figure 3:Flow cytometric analysis of Raji cells us- ing HIST2H4A(20Me3) mouse mAb (green) and negative control (red).

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]