DOCSLIB.ORG

Thesis Reference

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Thesis Reference Thesis Développement d'une méthodologie et optimisation d'un test colorimétrique pour la recherche de substances antimalariques d'origine végétale VARGAS, Sandra Abstract Ce travail présente une méthodologie pour la recherche de substances antimalariques d'origine naturelle. Elle implique l'utilisation des deux outils développés pour cette étude : un outil qualitatif (β-test) et une base de données (Track2nd). Le β-test est un test colorimétrique basé sur l'étude de la synthèse de la β-hématine, réaction spécifique au Plasmodium. Sa mise en place et son optimisation sur des extraits et des substances d'origine végétale permet d'avoir à disposition un test facile, rapide et peu couteux. Track2nd est un portail d'informations sur des plantes incluant des paramètres comme l'activité biologique et l'utilisation en médecine traditionnelle. Ces deux outils combinés permettent une première sélection d'extraits de plantes destinés à être testés sur le parasite par des instituts collaborateurs. Une fois la confirmation d'activité obtenue, un bioguidage avec le β-test est effectué afin d'isoler puis d'identifier le composé inhibiteur de la synthèse de la β-hématine. Reference VARGAS, Sandra. Développement d'une méthodologie et optimisation d'un test colorimétrique pour la recherche de substances antimalariques d'origine végétale. Thèse de doctorat : Univ. Genève, 2009, no. Sc. 4132 URN : urn:nbn:ch:unige-127440 DOI : 10.13097/archive-ouverte/unige:12744 Available at: http://archive-ouverte.unige.ch/unige:12744 Disclaimer: layout of this document may differ from the published version. 1 / 1 UNIVERSITE DE GENEVE FACULTE DES SCIENCES Section des Sciences Pharmaceutiques Laboratoire de Pharmacognosie et de Phytochimie Prof. Kurt Hostettmann Développement d'une méthodologie et optimisation d'un test colorimétrique pour la recherche de substances antimalariques d'origine végétale THESE Présentée à la Faculté des Sciences de l’Université de Genève Pour obtenir le grade de Docteur ès sciences, mention interdisciplinaire par Sandra Vargas de Meyrin (GE) Thèse No4132 Atelier d’impression ReproMail – Uni Mail Genève 2009 Remerciements Mes remerciements s’adressent tout d’abord à mon directeur de thèse, Monsieur le Professeur Kurt Hostettmann, pour m’avoir accueillie dans son groupe de recherche de renommée internationale et pour les conditions de travail de grande qualité dont j’ai pu bénéficier. Je le remercie également pour m’avoir permis d’intégrer pendant deux mois le laboratoire de Madame le Dr. Livia Vivas au sein de la London School of Hygien and Tropical Medicine (LSHTM), afin de parfaire mes connaissances concernant les tests in vitro avec P. falciparum. C’est également pour la confiance qu’il m’a accordée en me permettant de présenter mes résultats à différents congrès scientifiques en Suisse mais également en Indonésie et au Botswana que je lui présente ma profonde reconnaissance. Ma gratitude va également à mes superviseurs techniques. Ce sujet n’aurait pas vu le jour sans la détermination de Monsieur le Dr. Jean-Robert Ioset, que je remercie pour son encadrement lors de la mise en place du test, pour les nombreuses réunions et pour son optimisme. Un grand merci à Madame le Dr. Anne-Emmanuelle Hay pour son encadrement, son dynamisme, sa participation aux screenings ainsi que pour son soutien. Mes remerciements s’adressent aussi à Madame le Dr. Karine Ndjoko pour ses précieux conseils lors de la rédaction. Mes remerciement sincères s’adressent également à mon jury de thèse : Monsieur le Professeur Drissa Diallo, Chef du Département Médecine Traditionnelle (DMT) à l’Institut National de Recherche en Santé Publique (INRSP) de Bamako au Mali, Monsieur le Professeur Louis Maes du « Laboratory for Microbiology, Parasitology and Hygiene (LMPH) » de l’Université d’Antwerp en Belgique et Monsieur le Dr. Jean-Robert Ioset de l’organisation « Drugs for Neglected Diseases initiative » (DNDi) pour avoir accepté de juger mon travail, pour leurs remarques pertinentes et pour leur déplacement lors de la soutenance de thèse. Je remercie sincèrement Monsieur le Professeur Pierre-Alain Carrupt pour avoir accepté d’être membre du jury interne et pour avoir présider ce jury. Ce travail n’aurait pas pu être achevé sans les collaborations suivantes : L’Institut Tropical Suisse (STI) à Bâle, Monsieur le Professeur Reto Brun et plus particulièrement Monsieur le Dr. Sergio Wittlin pour les résultats in vitro sur P. falciparum de toutes les plantes sélectionnées lors de ce travail. La fondation Oswaldo Cruz (FioCruz) du Brésil, particulièrement Madame le Professeur Milena B. Soares pour avoir testé in vitro sur P. falciparum les fractions et substances pures provenant de Loiseleuria procumbens. Madame le Dr. Livia Vivas du LSHTM qui m’a accueilli dans son laboratoire et m’a permis de pratiquer les tests in vitro sur P. falciparum. Un grand merci à Mlle Emily Bongart et Mlle Eloise Thompson pour leur aide et pour leurs conseils au laboratoire. Monsieur le Dr. Serge Rudaz du Laboratoire d’Analyse Pharmaceutique de l’Université de Genève, qui a toujours été disponible afin de discuter du traitement des résultats. i Ma profonde reconnaissance va également aux deux étudiantes qui ont participé activement à ce travail dans le cadre de leur travail de Master en Pharmacie. Mlle Simone Egger et Mlle Aziza Ben Zayed qui par leur détermination ont énormément participé à la mise en place du β-test ainsi qu’au premier bioguidage. Je remercie également mes anciens collègues du Laboratoire de Pharmacognosie et Phytochimie : En particulier, Daphné : « Avec quelques semaines de décalage, on peut dire qu’on a vécu notre thèse en même temps ». Frédéric : « Il est vrai que certaine fois, on peut voir les choses sous un autre angle ». Sylvian : « Un véritable soutien optimiste ». Je les remercie pour les échanges scientifiques mais aussi pour nos fous rires et leur amitié. Anne- Emmanuelle pour sa bonne humeur, Martine pour le vendredi, Philippe C. pour nos discussions mais aussi Caroline, Anne-Laure, Fan, Karine, Gaetan, Trixie, Claudia, Raimana, Karin, Hakim, Aude et Nadine. Finalement, un grand merci à mes parents, ma sœur, mon frère et mes amis pour m’avoir encouragée et soutenue tout au long de ce travail. ii Communications scientifiques Ce travail a été effectué d’octobre 2005 à juin 2009 au Laboratoire de Pharmacognosie et Phytochimie, Section Sciences Pharmaceutiques à l’Université de Genève-Université de Lausanne, sous la direction du Prof. Dr. Kurt Hostettmann. Certains aspects de la présente recherche ont abouti à des présentations lors de congrès internationaux sous forme de posters ou de communications orales et à la publication d’articles. Posters Martin, F. Hay, A.E., Vargas, S., Gupta, M.P. and Hostettmann, K., New C- glucosylxanthones forme the stem of Arrabidaea patellifera (Bignoniaceae). International Symposium by IOCD, “Biology, Chemistry, Pharmacology and Clinical studies of Asian plants”, Surabaya, Indonesia. April 2007 Vargas, S., Ioset, J.-R., Hay, A.-E, Ndjoko K., Vivas, L., Hostettmann, K., A screening assay based on inhibition of hematin polymerization for detecting new antimalarial drugs: Application to plant extracts, International Symposium by IOCD, “Biology, Chemistry, Pharmacology and Clinical studies of Asian plants”, Surabaya, Indonesia. April 2007 Vargas, S., Ioset, J.-R., Hay, A.-E, Ndjoko K., Vivas, L., Hostettmann, K., β-hematin inhibition assay: A tool for detecting new antimalarial drugs from plants, IOCD/ISDNP Symposium ‘Natural Products: Unlimited Resources for the Development of Drugs, Cosmetics and Food.’, Kasane, Botswana, February 2008. Communications orales Vargas, S., Recherche de composés anti-malariques, Test colorimétrique basé sur l’inhibition de la polymérisation de l’hématine, 22ème Séminaire en Sciences Pharmaceutiques, « Le potentiel des plantes dans les maladies parasitaires tropicales », Zermatt, Suisse. Octobre 2007 Publications Simões-Pires, C., Vargas S., Marston A., Ioset J.R., Paulo M.Q., Matheeussen A. ; Maes L., Ellagic acid derivatives from Syzygium cumini stem bark: investigation of their antiplasmodial activity, Natural Product Communications, 2009 (4), Soumis Vargas, S., Ioset, J.-R., Hay, A.-E, Ndjoko, K., Brun, R., Wittlin, S., Hostettmann, K., The adaptation and validation of use of a chemical assay based on the inhibition of the beta-hematin to the screening of natural products: a simple method for the detection and selectively isolation of novel antimalarial compounds from plant mixtures. Soumis iii Abréviations et Symboles [3H]-Hypoxanthine Hypoxanthine radiomarquée au tritium δ Déplacement chimique en RMN η Rendement λmax Longueur d’onde du maxima d’absorption ABS Absorption ACN Acétonitrile CD3OD Methanol deutéré CI50 Concentration lorsque l’inhibition est équivalente à 50 % COSY Correlation homonucléaire d Doublet DAD Detecteur à barettes d’iodes dd Doublet de doublet DDT Dichloro-diphenyl-trichloroéthane DMSO Dimethylsulfoxide DMSO-d6 Dimethylsulfoxide deutéré DNDi Drugs for Neglected Diseases initiative (organisation à but non-lucratif ) DPPH 1,1- diphenyl-2-picrylhydrazyl ELISA Dosage d'immunosorption liée à enzyme (Enzyme-linked immunosorbent assay) ESI Ionisation par électrospray et al. « et autres », abbrévitation du mot latin et alii Fe(III)PPIX Fer (III) lié à la protoporphyrine IX FT Transformée de Fourier GSH Glutathion HMBC Correlation hétéronucléaire à liaisons multiples HPLC Chromatographie liquide à haute pression HR-MS Spéctrométrie de masse à haute résolution HSQC Corrélation hétéronuclaire
Load more

Recommended publications
  • Systematic Studies of the South African Campanulaceae Sensu Stricto with an Emphasis on Generic Delimitations
    Town The copyright of this thesis rests with the University of Cape Town. No quotation from it or information derivedCape from it is to be published without full acknowledgement of theof source. The thesis is to be used for private study or non-commercial research purposes only. University Systematic studies of the South African Campanulaceae sensu stricto with an emphasis on generic delimitations Christopher Nelson Cupido Thesis presented for the degree of DOCTOR OF PHILOSOPHY in the Department of Botany Town UNIVERSITY OF CAPECape TOWN of September 2009 University Roella incurva Merciera eckloniana Microcodon glomeratus Prismatocarpus diffusus Town Wahlenbergia rubioides Cape of Wahlenbergia paniculata (blue), W. annularis (white) Siphocodon spartioides University Rhigiophyllum squarrosum Wahlenbergia procumbens Representatives of Campanulaceae diversity in South Africa ii Town Dedicated to Ursula, Denroy, Danielle and my parents Cape of University iii Town DECLARATION Cape I confirm that this is my ownof work and the use of all material from other sources has been properly and fully acknowledged. University Christopher N Cupido Cape Town, September 2009 iv Systematic studies of the South African Campanulaceae sensu stricto with an emphasis on generic delimitations Christopher Nelson Cupido September 2009 ABSTRACT The South African Campanulaceae sensu stricto, comprising 10 genera, represent the most diverse lineage of the family in the southern hemisphere. In this study two phylogenies are reconstructed using parsimony and Bayesian methods. A family-level phylogeny was estimated to test the monophyly and time of divergence of the South African lineage. This analysis, based on a published ITS phylogeny and an additional ten South African taxa, showed a strongly supported South African clade sister to the campanuloids.
    [Show full text]
  • Relationships Among Levels of Biodiversity and the Relevance of Intraspecific Diversity in Conservation – a Project Synopsis F
    ARTICLE IN PRESS Perspectives in Plant Ecology, Evolution and Systematics Perspectives in Plant Ecology, Evolution and Systematics 10 (2008) 259–281 www.elsevier.de/ppees Relationships among levels of biodiversity and the relevance of intraspecific diversity in conservation – a project synopsis F. Gugerlia,Ã, T. Englischb, H. Niklfeldb, A. Tribschc,1, Z. Mirekd, M. Ronikierd, N.E. Zimmermanna, R. Holdereggera, P. Taberlete, IntraBioDiv Consortium2,3 aWSL Swiss Federal Research Institute, Zu¨rcherstrasse 111, 8903 Birmensdorf, Switzerland bDepartment of Biogeography, University of Vienna, Rennweg 14, 1030 Wien, Austria cDepartment of Systematic and Evolutionary Botany, Rennweg 14, 1030 Wien, Austria dDepartment of Vascular Plant Systematics, Institute of Botany, Polish Academy of Science, Krako´w, Lubicz 46, 31-512 Krako´w, Poland eLaboratoire d’Ecologie Alpine (LECA), CNRS UMR 5553, University Joseph Fourier, BP 53, 2233 Rue de la Piscine, 38041 Grenoble Cedex 9, France Received 11 June 2007; received in revised form 4 June 2008; accepted 9 July 2008 Abstract The importance of the conservation of all three fundamental levels of biodiversity (ecosystems, species and genes) has been widely acknowledged, but only in recent years it has become technically feasible to consider intraspecific diversity, i.e. the genetic component to biodiversity. In order to facilitate the assessment of biodiversity, considerable efforts have been made towards identifying surrogates because the efficient evaluation of regional biodiversity would help in designating important areas for nature conservation at larger spatial scales. However, we know little about the fundamental relationships among the three levels of biodiversity, which impedes the formulation of a general, widely applicable concept of biodiversity conservation through surrogates.
    [Show full text]
  • Traditional Medicinal Plants of Nigeria: an Overview Monier M
    AGRICULTURE AND BIOLOGY JOURNAL OF NORTH AMERICA ISSN Print: 2151-7517, ISSN Online: 2151-7525, doi:10.5251/abjna.2016.7.5.220.247 © 2016, ScienceHuβ, http://www.scihub.org/ABJNA Traditional medicinal plants of Nigeria: an overview Monier M. Abd El-Ghani12* 1 Biology Department, Faculty of Natural and Applied Sciences, Umaru, Musa Yar’adua University, Katsina State, Federal Republic of Nigeria 2 Permanent Address: Department of Botany and Microbiology, Faculty of science, Cairo University, Giza 12613, Egypt Corresponding author:[email protected] ABSTRACT The present study represents an attempt to document information on the traditional medicinal plants that used in Nigeria. A compiled check list of these plants including their Latin names, families, parts used, medicinal uses, and name in different Nigerian states is the main purpose of this study. All available information about either the traditional medicinal plants or ethnobotanical surveys in Nigeria was consulted. The study showed that 325 species and 95 families of medicinal plants were recognized as being used by most of the people in Nigeria for the treatment of various common diseases. Fabaceae has the largest number of species (42), followed by Asteraceae (22), Euphorbiaceae (20), Acanthaceae (13) and Apocynaceae (12). The largest genera were Euphorbia (6 species), Cola and Hibiscus (5 species for each), Albizia, Acacia, Combretum and Ficus (4 species for each), Acalypha, Allium, Clerodendrum and Cleome (3 species for each). The study revealed that traditional medicinal practices have a wide acceptability among the Nigerian people, probably because they believe in its effectiveness. The medicinal uses are varied, and the plant parts that are used ranged from leaves, roots, stem, bark to fruits only, or a combination of two or more in a species or with those of other species.
    [Show full text]
  • Extracts from Sageretia Thea Reduce Cell Viability Through Inducing Cyclin
    Kim et al. BMC Complementary and Alternative Medicine (2019) 19:43 https://doi.org/10.1186/s12906-019-2453-4 RESEARCH ARTICLE Open Access Extracts from Sageretia thea reduce cell viability through inducing cyclin D1 proteasomal degradation and HO-1 expression in human colorectal cancer cells Ha Na Kim1†, Gwang Hun Park2†, Su Bin Park1, Jeong Dong Kim1, Hyun Ji Eo2, Ho-Jun Son2, Jeong Ho Song2 and Jin Boo Jeong1,3* Abstract Background: Sageretia thea (S. thea) has been used as the medicinal plant for treating hepatitis and fevers in Korea and China. Recently, anticancer activity of S. thea has been reported, but the potential mechanism for the anti- cancer property of S. thea is still insufficient. Thus, we evaluated whether extracts from the leaves (STL) and branches (STB) of S. thea exert anticancer activity and elucidated its potential mechanism in SW480 cells. Methods: MTT assay was performed for measuring cell viability. Western blot and RT-PCR were used for analyzing the level of protein and mRNA, respectively. Results: Treatment of STL or STB decreased the cell viability and induced apoptosis in SW480 cells. Decreased level of cyclin D1 protein was observed in SW480 cells treated with STL or STB, but no change in cyclin D1 mRNA level was observed with the treatment of STL or STB. MG132 blocked downregulation of cyclin D1 protein by STL or STB. Thr286 phosphorylation of cyclin D1 by STL or STB occurred faster than downregulation of cyclin D1 protein in SW480 cells. When SW480 cells were transfected with T286A-cyclin D1, cyclin D1 degradation by STL or STB did not occur.
    [Show full text]
  • Springs and Springs-Dependent Taxa of the Colorado River Basin, Southwestern North America: Geography, Ecology and Human Impacts
    water Article Springs and Springs-Dependent Taxa of the Colorado River Basin, Southwestern North America: Geography, Ecology and Human Impacts Lawrence E. Stevens * , Jeffrey Jenness and Jeri D. Ledbetter Springs Stewardship Institute, Museum of Northern Arizona, 3101 N. Ft. Valley Rd., Flagstaff, AZ 86001, USA; Jeff@SpringStewardship.org (J.J.); [email protected] (J.D.L.) * Correspondence: [email protected] Received: 27 April 2020; Accepted: 12 May 2020; Published: 24 May 2020 Abstract: The Colorado River basin (CRB), the primary water source for southwestern North America, is divided into the 283,384 km2, water-exporting Upper CRB (UCRB) in the Colorado Plateau geologic province, and the 344,440 km2, water-receiving Lower CRB (LCRB) in the Basin and Range geologic province. Long-regarded as a snowmelt-fed river system, approximately half of the river’s baseflow is derived from groundwater, much of it through springs. CRB springs are important for biota, culture, and the economy, but are highly threatened by a wide array of anthropogenic factors. We used existing literature, available databases, and field data to synthesize information on the distribution, ecohydrology, biodiversity, status, and potential socio-economic impacts of 20,872 reported CRB springs in relation to permanent stream distribution, human population growth, and climate change. CRB springs are patchily distributed, with highest density in montane and cliff-dominated landscapes. Mapping data quality is highly variable and many springs remain undocumented. Most CRB springs-influenced habitats are small, with a highly variable mean area of 2200 m2, generating an estimated total springs habitat area of 45.4 km2 (0.007% of the total CRB land area).
    [Show full text]
  • A Global Method for Calculating Plant CSR Ecological Strategies Applied Across Biomes Worldwide” in Functional Ecology, Vol
    Post-Print of: Pierce, Simon et al. “A Global method for calculating plant CSR ecological strategies applied across biomes worldwide” in Functional Ecology, vol. 31 num. 2 (2017), p. 444-457. The final version is available at DOI 10.1111/1365-2435.12722 A global method for calculating plant CSR ecological strategies 2 applied across biomes worldwide 3 Simon Pierce1, Daniel Negreiros2, Bruno E.L. Cerabolini3, Jens Kattge4, Sandra Díaz5, Michael Kleyer6, Bill 4 Shipley7, S. Joseph Wright8, Nadejda A. Soudzilovskaia9, Vladimir G. Onipchenko10, Peter M. van Bodegom9, 5 Cedric Frenette-Dussault7, Evan Weiher12, Bruno X. Pinho13, Johannes H.C. Cornelissen11, J. Philip Grime14, Ken 6 Thompson14; Roderick Hunt15, Peter J. Wilson14; Gabriella Buffa16, Oliver C. Nyakunga16,17, Peter B. Reich18,19, 7 Marco Caccianiga20, Federico Mangili20, Roberta M. Ceriani21, Alessandra Luzzaro1, Guido Brusa3, Andrew 8 Siefert22, Newton P.U. Barbosa2, F. Stuart Chapin III23, William K. Cornwell24, Jingyun Fang25, G. Wilson 9 Fernandes2,26, Eric Garnier27, Soizig Le Stradic28, Josep Peñuelas29,30, Felipe P. L. Melo13, Antonio Slaviero16, 10 Marcelo Tabarelli13, Duccio Tampucci20. 11 12 1 Department of Agricultural and Environmental Sciences (DiSAA), University of Milan, Via G. Celoria 2, I-20133 13 Milan, Italy; 14 2 Ecologia Evolutiva e Biodiversidade/DBG, CP 486, ICB/Universidade Federal de Minas Gerais, 30161 -970. 15 Belo Horizonte, MG, Brazil; 16 3 Department of Theoretical and Applied Sciences, University of Insubria, Via J.H. Dunant 3, I-21100 Varese, 17
    [Show full text]
  • The One Hundred Tree Species Prioritized for Planting in the Tropics and Subtropics As Indicated by Database Mining
    The one hundred tree species prioritized for planting in the tropics and subtropics as indicated by database mining Roeland Kindt, Ian K Dawson, Jens-Peter B Lillesø, Alice Muchugi, Fabio Pedercini, James M Roshetko, Meine van Noordwijk, Lars Graudal, Ramni Jamnadass The one hundred tree species prioritized for planting in the tropics and subtropics as indicated by database mining Roeland Kindt, Ian K Dawson, Jens-Peter B Lillesø, Alice Muchugi, Fabio Pedercini, James M Roshetko, Meine van Noordwijk, Lars Graudal, Ramni Jamnadass LIMITED CIRCULATION Correct citation: Kindt R, Dawson IK, Lillesø J-PB, Muchugi A, Pedercini F, Roshetko JM, van Noordwijk M, Graudal L, Jamnadass R. 2021. The one hundred tree species prioritized for planting in the tropics and subtropics as indicated by database mining. Working Paper No. 312. World Agroforestry, Nairobi, Kenya. DOI http://dx.doi.org/10.5716/WP21001.PDF The titles of the Working Paper Series are intended to disseminate provisional results of agroforestry research and practices and to stimulate feedback from the scientific community. Other World Agroforestry publication series include Technical Manuals, Occasional Papers and the Trees for Change Series. Published by World Agroforestry (ICRAF) PO Box 30677, GPO 00100 Nairobi, Kenya Tel: +254(0)20 7224000, via USA +1 650 833 6645 Fax: +254(0)20 7224001, via USA +1 650 833 6646 Email: [email protected] Website: www.worldagroforestry.org © World Agroforestry 2021 Working Paper No. 312 The views expressed in this publication are those of the authors and not necessarily those of World Agroforestry. Articles appearing in this publication series may be quoted or reproduced without charge, provided the source is acknowledged.
    [Show full text]
  • Index Seminum 2018-2019
    UNIVERSITÀ DEGLI STUDI DI NAPOLI FEDERICO II ORTO BOTANICO INDEX SEMINUM 2018-2019 In copertina / Cover “La Terrazza Carolina del Real Orto Botanico” Dedicata alla Regina Maria Carolina Bonaparte da Gioacchino Murat, Re di Napoli dal 1808 al 1815 (Photo S. Gaudino, 2018) 2 UNIVERSITÀ DEGLI STUDI DI NAPOLI FEDERICO II ORTO BOTANICO INDEX SEMINUM 2018 - 2019 SPORAE ET SEMINA QUAE HORTUS BOTANICUS NEAPOLITANUS PRO MUTUA COMMUTATIONE OFFERT 3 UNIVERSITÀ DEGLI STUDI DI NAPOLI FEDERICO II ORTO BOTANICO ebgconsortiumindexseminum2018-2019 IPEN member ➢ CarpoSpermaTeca / Index-Seminum E- mail: [email protected] - Tel. +39/81/2533922 Via Foria, 223 - 80139 NAPOLI - ITALY http://www.ortobotanico.unina.it/OBN4/6_index/index.htm 4 Sommario / Contents Prefazione / Foreword 7 Dati geografici e climatici / Geographical and climatic data 9 Note / Notices 11 Mappa dell’Orto Botanico di Napoli / Botanical Garden map 13 Legenda dei codici e delle abbreviazioni / Key to signs and abbreviations 14 Index Seminum / Seed list: Felci / Ferns 15 Gimnosperme / Gymnosperms 18 Angiosperme / Angiosperms 21 Desiderata e condizioni di spedizione / Agreement and desiderata 55 Bibliografia e Ringraziamenti / Bibliography and Acknowledgements 57 5 INDEX SEMINUM UNIVERSITÀ DEGLI STUDI DI NAPOLI FEDERICO II ORTO BOTANICO Prof. PAOLO CAPUTO Horti Praefectus Dr. MANUELA DE MATTEIS TORTORA Seminum curator STEFANO GAUDINO Seminum collector 6 Prefazione / Foreword L'ORTO BOTANICO dell'Università ha lo scopo di introdurre, curare e conservare specie vegetali da diffondere e proteggere,
    [Show full text]
  • Flora of China 12: 133–139. 2007. 7. SAGERETIA Brongniart, Mém. Fam
    Flora of China 12: 133–139. 2007. 7. SAGERETIA Brongniart, Mém. Fam. Rhamnées, 52. 1826. 雀梅藤属 que mei teng shu Ampeloplis Rafinesque. Shrubs scandent or erect, rarely small trees, unarmed or spinescent. Branchlets alternate or subopposite, often terminating in a woody spine. Leaves alternate or subopposite; stipules small, caducous; leaf blade papery to leathery, pinnately veined, margin ser- rate, rarely entire. Flowers mostly very small, 1–2 mm in diam., bisexual, 5-merous, usually sessile or subsessile, rarely pedicellate, in spikes or spicate panicles, rarely in racemes. Calyx tube shallowly cup-shaped to hemispherical; sepals triangular, ± fleshy, adax- ially medially keeled and hooded. Petals spatulate, apex 2-lobed to ± deeply emarginate. Stamens equaling petals or slightly longer; anthers dorsifixed. Disk cup-shaped, thick, fleshy, outer margin free from calyx tube, ± distinctly erect, entire or 5-lobed. Ovary superior, 2- or 3-loculed, with 1 ovule per locule; style short, stout, undivided, apically ± distinctly 2- or 3-lobed. Drupe obovoid- globose, with 2 or 3 one-seeded stones, base with remnants of persistent calyx tube. Seeds compressed, slightly asymmetrical, concave at both ends. About 35 species: mainly in SE Asia, a few species in Africa and North America; 19 species (15 endemic) in China. Sageretia gongshanensis G. S. Fan & L. L. Deng (Sida 17: 677. 1997) and S. latifolia Handel-Mazzetti (Symb. Sin. 7: 673. 1933), both described from Yunnan, and S. yunlongensis G. S. Fan & L. L. Deng (Sida 16: 477. 1995), described from Xizang and Yunnan, could not be treated here because no material was seen by the authors.
    [Show full text]
  • Medicinal Plants of Otwal and Ngai Sub Counties in Oyam District, Northern Uganda Maud M Kamatenesi1†, Annabel Acipa2*, Hannington Oryem-Origa1†
    Kamatenesi et al. Journal of Ethnobiology and Ethnomedicine 2011, 7:7 http://www.ethnobiomed.com/content/7/1/7 JOURNAL OF ETHNOBIOLOGY AND ETHNOMEDICINE RESEARCH Open Access Medicinal plants of Otwal and Ngai Sub Counties in Oyam District, Northern Uganda Maud M Kamatenesi1†, Annabel Acipa2*, Hannington Oryem-Origa1† Abstract Background: An ethnobotanical study was carried out in four parishes in the Ngai and Otwal Sub Counties in Oyam district, Northern Uganda, where insurgency has been prevalent for the past 20 years. Documenting medicinal plant species used in treating various health conditions among the local people. Methods: Information was obtained from mainly the local population, the traditional healers and other experienced persons through interviews, formal and informal discussions and field excursions. Results: Seventy one plant species were reported for use in the treatment of various diseases in the study area. These plant species belongs to 41 families, with Asteraceae being the most represented. Roots were ranked the commonest plant part used. Oral administration was the most frequently used route of administration. A total of 41 different health conditions were reported to be treated by use of medicinal plant species. Thirty nine percent of the recorded plant species were reported for treating stomach related ailments. Conclusion: The use of medicinal plants in primary healthcare is still a common practice in Ngai and Otwal Sub Counties. The trust they have is built on the curative outcome properties claimed, poverty and armed conflict that lead to inadequate healthcare facilities. The generation gap caused by the over 20 years of insurgency in the area has brought about knowledge gap on the usage of medicinal plant species between the young and the older generation.
    [Show full text]
  • Natural Products As a Source for Treating Neglected Parasitic Diseases
    Int. J. Mol. Sci. 2013, 14, 3395-3439; doi:10.3390/ijms14023395 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Review Natural Products as a Source for Treating Neglected Parasitic Diseases Dieudonné Ndjonka 1,†, Ludmila Nakamura Rapado 2,†, Ariel M. Silber 2, Eva Liebau 3,* and Carsten Wrenger 2,* 1 Department of Biological Sciences, Faculty of Science, University of Ngaoundere, B. P. 454, Cameroon; E-Mail: [email protected] 2 Unit for Drug Discovery, Department of Parasitology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes 1374, 05508-000 São Paulo-SP, Brazil; E-Mails: [email protected] (L.N.R.); [email protected] (A.M.S.) 3 Institute for Zoophysiology, Schlossplatz 8, D-48143 Münster, Germany † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: [email protected] (E.L.); [email protected] (C.W.); Tel.: +49-251-83-21710 (E.L.); +55-11-3091-7335 (C.W.); Fax: +49-251-83-21766 (E.L.); +55-11-3091-7417 (C.W.). Received: 21 December 2012; in revised form: 12 January 2013 / Accepted: 16 January 2013 / Published: 6 February 2013 Abstract: Infectious diseases caused by parasites are a major threat for the entire mankind, especially in the tropics. More than 1 billion people world-wide are directly exposed to tropical parasites such as the causative agents of trypanosomiasis, leishmaniasis, schistosomiasis, lymphatic filariasis and onchocerciasis, which represent a major health problem, particularly in impecunious areas. Unlike most antibiotics, there is no “general” antiparasitic drug available.
    [Show full text]
  • Lamiales – Synoptical Classification Vers
    Lamiales – Synoptical classification vers. 2.6.2 (in prog.) Updated: 12 April, 2016 A Synoptical Classification of the Lamiales Version 2.6.2 (This is a working document) Compiled by Richard Olmstead With the help of: D. Albach, P. Beardsley, D. Bedigian, B. Bremer, P. Cantino, J. Chau, J. L. Clark, B. Drew, P. Garnock- Jones, S. Grose (Heydler), R. Harley, H.-D. Ihlenfeldt, B. Li, L. Lohmann, S. Mathews, L. McDade, K. Müller, E. Norman, N. O’Leary, B. Oxelman, J. Reveal, R. Scotland, J. Smith, D. Tank, E. Tripp, S. Wagstaff, E. Wallander, A. Weber, A. Wolfe, A. Wortley, N. Young, M. Zjhra, and many others [estimated 25 families, 1041 genera, and ca. 21,878 species in Lamiales] The goal of this project is to produce a working infraordinal classification of the Lamiales to genus with information on distribution and species richness. All recognized taxa will be clades; adherence to Linnaean ranks is optional. Synonymy is very incomplete (comprehensive synonymy is not a goal of the project, but could be incorporated). Although I anticipate producing a publishable version of this classification at a future date, my near- term goal is to produce a web-accessible version, which will be available to the public and which will be updated regularly through input from systematists familiar with taxa within the Lamiales. For further information on the project and to provide information for future versions, please contact R. Olmstead via email at [email protected], or by regular mail at: Department of Biology, Box 355325, University of Washington, Seattle WA 98195, USA.
    [Show full text]