Sequential Organizing Activities of Engrailed, Hedgehog and Decapentaplegic in the Drosophila Wing
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T-Box Genes in Limb Development and Disease
Open Research Online The Open University’s repository of research publications and other research outputs T-box Genes in Limb Development and Disease Thesis How to cite: Rallis, Charalampos (2004). T-box Genes in Limb Development and Disease. PhD thesis The Open University. For guidance on citations see FAQs. c 2004 Charalampos Rallis Version: Version of Record Link(s) to article on publisher’s website: http://dx.doi.org/doi:10.21954/ou.ro.0000fa0b Copyright and Moral Rights for the articles on this site are retained by the individual authors and/or other copyright owners. For more information on Open Research Online’s data policy on reuse of materials please consult the policies page. oro.open.ac.uk T-box Genes in Limb Development and Disease Charalampos Rallis Thesis submitted for the degree of Doctor of Philosophy October 2004 Division of Developmental Biology National Institute for Medical Research Mill Hill London Open University ProQuest Number: C819643 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a com plete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest C819643 Published by ProQuest LLO (2019). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States C ode Microform Edition © ProQuest LLO. ProQuest LLO. 789 East Eisenhower Parkway P.Q. -
Pancreas-Specific Deletion of Mouse Gata4 and Gata6 Causes Pancreatic Agenesis
Pancreas-specific deletion of mouse Gata4 and Gata6 causes pancreatic agenesis Shouhong Xuan, … , Raymond J. Macdonald, Lori Sussel J Clin Invest. 2012;122(10):3516-3528. https://doi.org/10.1172/JCI63352. Research Article Pancreatic agenesis is a human disorder caused by defects in pancreas development. To date, only a few genes have been linked to pancreatic agenesis in humans, with mutations in pancreatic and duodenal homeobox 1 (PDX1) and pancreas-specific transcription factor 1a (PTF1A) reported in only 5 families with described cases. Recently, mutations in GATA6 have been identified in a large percentage of human cases, and aG ATA4 mutant allele has been implicated in a single case. In the mouse, Gata4 and Gata6 are expressed in several endoderm-derived tissues, including the pancreas. To analyze the functions of GATA4 and/or GATA6 during mouse pancreatic development, we generated pancreas- specific deletions of Gata4 and Gata6. Surprisingly, loss of either Gata4 or Gata6 in the pancreas resulted in only mild pancreatic defects, which resolved postnatally. However, simultaneous deletion of both Gata4 and Gata6 in the pancreas caused severe pancreatic agenesis due to disruption of pancreatic progenitor cell proliferation, defects in branching morphogenesis, and a subsequent failure to induce the differentiation of progenitor cells expressing carboxypeptidase A1 (CPA1) and neurogenin 3 (NEUROG3). These studies address the conserved and nonconserved mechanisms underlying GATA4 and GATA6 function during pancreas development and provide a new mouse model to characterize the underlying developmental defects associated with pancreatic agenesis. Find the latest version: https://jci.me/63352/pdf Research article Related Commentary, page 3469 Pancreas-specific deletion of mouse Gata4 and Gata6 causes pancreatic agenesis Shouhong Xuan,1 Matthew J. -
Engrailed Homeoproteins in Visual System Development
Cell. Mol. Life Sci. (2015) 72:1433–1445 DOI 10.1007/s00018-014-1776-z Cellular and Molecular Life Sciences REVIEW Engrailed homeoproteins in visual system development Andrea Wizenmann • Olivier Stettler • Kenneth L. Moya Received: 28 July 2014 / Revised: 31 October 2014 / Accepted: 6 November 2014 / Published online: 29 November 2014 Ó The Author(s) 2014. This article is published with open access at Springerlink.com Abstract Engrailed is a homeoprotein transcription Keywords Visual system Á Retina Á Tectum Á factor. This family of transcription factors is characterized Sensory map Á Homeoprotein Á Engrailed by their DNA-binding homeodomain and some members, including Engrailed, can transfer between cells and reg- ulate protein translation in addition to gene transcription. Introduction Engrailed is intimately involved in the development of the vertebrate visual system. Early expression of Engrailed in Since the discovery of homeobox genes [1, 2] there has dorsal mesencephalon contributes to the development and been accumulating evidence from all multi-cellular organization of a visual structure, the optic tectum/supe- organisms that these genes play key roles in determining rior colliculus. This structure is an important target for positional information. These genes encode homeoprotein retinal ganglion cell axons that carry visual information transcription factors that regulate the expression of down- from the retina. Engrailed regulates the expression of stream genes necessary at all developmental stages, Ephrin axon guidance cues in the tectum/superior col- including lineage determination, cell migration, cell dif- liculus. More recently it has been reported that Engrailed ferentiation, and tissue formation. Some homeoproteins are itself acts as an axon guidance cue in synergy with the also able to regulate protein translation and cell-to-cell Ephrin system and is proposed to enhance retinal topo- signaling. -
Transcriptional and Epigenetic Control of Brown and Beige Adipose Cell Fate and Function
REVIEWS Transcriptional and epigenetic control of brown and beige adipose cell fate and function Takeshi Inagaki1,2, Juro Sakai1,2 and Shingo Kajimura3 Abstract | White adipocytes store excess energy in the form of triglycerides, whereas brown and beige adipocytes dissipate energy in the form of heat. This thermogenic function relies on the activation of brown and beige adipocyte-specific gene programmes that are coordinately regulated by adipose-selective chromatin architectures and by a set of unique transcriptional and epigenetic regulators. A number of transcriptional and epigenetic regulators are also required for promoting beige adipocyte biogenesis in response to various environmental stimuli. A better understanding of the molecular mechanisms governing the generation and function of brown and beige adipocytes is necessary to allow us to control adipose cell fate and stimulate thermogenesis. This may provide a therapeutic approach for the treatment of obesity and obesity-associated diseases, such as type 2 diabetes. Interscapular BAT Adipose tissue has a central role in whole-body energy subjects who had previously lacked detectable BAT Brown adipose tissue (BAT) is a homeostasis. White adipose tissue (WAT) is the major depots before cold exposure, presumably owing to the specialized organ that adipose organ in mammals. It represents 10% or more emergence of new thermogenic adipocytes. This, then, produces heat. BAT is localized of the body weight of healthy adult humans and is leads to an increase in non-shivering thermogenesis in the interscapular and 6–9 perirenal regions of rodents specialized for the storage of excess energy. Humans and/or an improvement in insulin sensitivity . These and infants. -
PAX Genes in Childhood Oncogenesis: Developmental Biology Gone Awry?
Oncogene (2015) 34, 2681–2689 © 2015 Macmillan Publishers Limited All rights reserved 0950-9232/15 www.nature.com/onc REVIEW PAX genes in childhood oncogenesis: developmental biology gone awry? P Mahajan1, PJ Leavey1 and RL Galindo1,2,3 Childhood solid tumors often arise from embryonal-like cells, which are distinct from the epithelial cancers observed in adults, and etiologically can be considered as ‘developmental patterning gone awry’. Paired-box (PAX) genes encode a family of evolutionarily conserved transcription factors that are important regulators of cell lineage specification, migration and tissue patterning. PAX loss-of-function mutations are well known to cause potent developmental phenotypes in animal models and underlie genetic disease in humans, whereas dysregulation and/or genetic modification of PAX genes have been shown to function as critical triggers for human tumorigenesis. Consequently, exploring PAX-related pathobiology generates insights into both normal developmental biology and key molecular mechanisms that underlie pediatric cancer, which are the topics of this review. Oncogene (2015) 34, 2681–2689; doi:10.1038/onc.2014.209; published online 21 July 2014 INTRODUCTION developmental mechanisms and PAX genes in medical (adult) The developmental mechanisms necessary to generate a fully oncology. patterned, complex organism from a nascent embryo are precise. Undifferentiated primordia undergo a vast array of cell lineage specification, migration and patterning, and differentiate into an STRUCTURAL MOTIFS DEFINE THE PAX FAMILY SUBGROUPS ensemble of interdependent connective, muscle, nervous and The mammalian PAX family of transcription factors is comprised of epithelial tissues. Dysregulation of these precise developmental nine members that function as ‘master regulators’ of organo- programs cause various diseases/disorders, including—and genesis4 (Figure 1). -
Pax Genes and Their Roles in Cell Differentiation and Development Ahmed Mansouri*, Marc Hallonetl and Peter Gruss
851 Pax genes and their roles in cell differentiation and development Ahmed Mansouri*, Marc Hallonetl and Peter Gruss Members of the Pax gene family are expressed in various and in human aniridia [13]; in all of these, eye defects tissues during ontogenesis. Evidence for their crucial role are displayed. Pax2 is expressed during eye and kidney in morphogenesis, organogenesis, cell differentiation and development [14] and is mutated in a human family with oncogenesis is provided by rodent mutants and human kidney and eye abnormalities [15]. Pax genes clearly play diseases. Additionally, recent experimental in vivo and in vitro important roles during the formation of many structures. approaches have led to the identification of molecules that Furthermore, deregulated expression of Pax genes may interact with Pax proteins. lead to oncogenesis [16-18]. In this review, we focus on recent data documenting the Addresses role of Pax genes, and the interaction of the Pax proteins Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 G/Sttingen, with other transcription factors, in the development of the Germany nervous system, in organogenesis and in cell proliferation *e-mail: [email protected] and differentiation. re-mail: [email protected] Se-mail: [email protected] Pax genes in the central nervous system Current Opinion in Cell Biology 1996, 8:851-857 The roles of Pax genes are particularly documented at © Current Biology Lid ISSN 0955-0674 the level of the nervous system, which consequently may represent a model for the study of the function of the Pax Abbreviations BMP bone morphogenetic protein genes. -
Supplementary File Table S1
Supplementary file Table S1 Grouping of Homeobox genes according to their main known function. Anatomical Structure Morphogenesis EN1, HOXC10, HOXC13, HOXD3, LBX1, SIX2, SIX4 Organ Morphogenesis CDX1, CDX2, HOXA11, HOXA13, ISL1, LHX1, PAX3, PDHX, PITX2, PITX3, PROX1, SIX6 Body Pattern Formation ALX3, EMX2, HHEX, HOXA11, HOXA2, HOXA4, HOXA5, HOXA6, HOXB1, HOXB5, HOXB6, HOXC5, HOXD10, HOXD8, LMX1B, PITX2 Ectoderm Development PROX1, VAX2 Endoderm Development HOXC11 Brain & Nervous System Development Brain Development ALX1, DLX2, EMX2 Nervous System Development: ARX, DLX5, DLX6, HOXD10, LBX1, LHX1, OTP, PAX3, PHOX2A, PHOX2B Skeletal Development: ALX3, ALX4, DLX3, DLX5, DLX6, EN1, HOXA11, HOXA13, HOXA2, HOXB6, HOXD10, HOXD13, MSX2 Muscle Development: BARX2, MKX, SIRT1, SIRT2, SIX1 Other Homeobox Genes Involved In BARX1, CDX4, CUX1, DLX1, EMX1, EN2, Multicellular Organismal HOXA1, HOXA7, HOXA9, HOXB13, HOXB2, Development: HOXB3, HOXB4, HOXB7, HOXB8, HOXB9, HOXC12, HOXC8, HOXC9, HOXD1, HOXD11, HOXD12, HOXD9, ISL2, LBX2, LMX1A, MEIS1, NKX3-1, OTX1, TLX1, VAX1, VSX1, VSX2 Homeobox Genes Involved In Cell ARX, EMX2, HHEX, HLX, HOPX, LBX1, LHX1, Differentiation: LMX1B, MIXL1, OTP, PHOX2A, SIRT1, VSX2 Other Genes: PHTF1, SIRT3, SIRT6, SIRT7, ZHX1, ZHX2 Homeobox genes include two subsets of genes coding for transcription factors involved in multiple functions. The clustered HOX genes are indicated in bold. Supplementary file Figure S2 5’ Spatial collinearity 3’ HOXA Chr. 7p15.3 HOXB Chr. 17q21.3 HOXC Chr. 12q13.3 HOXD Chr. 2q31 13 12 11 10 9 8 7 6 5 4 3 2 1 Paralogous HOX groups Distribution of the 39 human HOX genes in four clusters located in different chromosomal regions*. Blue indicates anterior HOX genes. Yellow, paralogy group 3 Hox genes, green and purple indicatete central HOX genes and Red the posterior HOX genes. -
Positional Specificity of Different Transcription Factor Classes Within Enhancers
Positional specificity of different transcription factor classes within enhancers Sharon R. Grossmana,b,c, Jesse Engreitza, John P. Raya, Tung H. Nguyena, Nir Hacohena,d, and Eric S. Landera,b,e,1 aBroad Institute of MIT and Harvard, Cambridge, MA 02142; bDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139; cProgram in Health Sciences and Technology, Harvard Medical School, Boston, MA 02215; dCancer Research, Massachusetts General Hospital, Boston, MA 02114; and eDepartment of Systems Biology, Harvard Medical School, Boston, MA 02215 Contributed by Eric S. Lander, June 19, 2018 (sent for review March 26, 2018; reviewed by Gioacchino Natoli and Alexander Stark) Gene expression is controlled by sequence-specific transcription type-restricted enhancers (active in <50% of the cell types) and factors (TFs), which bind to regulatory sequences in DNA. TF ubiquitous enhancers (active in >90% of the cell types) (SI Ap- binding occurs in nucleosome-depleted regions of DNA (NDRs), pendix, Fig. S1C). which generally encompass regions with lengths similar to those We next sought to infer functional TF-binding sites within the protected by nucleosomes. However, less is known about where active regulatory elements. In a recent study (5), we found that within these regions specific TFs tend to be found. Here, we char- TF binding is strongly correlated with the quantitative DNA acterize the positional bias of inferred binding sites for 103 TFs accessibility of a region. Furthermore, the TF motifs associated within ∼500,000 NDRs across 47 cell types. We find that distinct with enhancer activity in reporter assays in a cell type corre- classes of TFs display different binding preferences: Some tend to sponded closely to those that are most enriched in the genomic have binding sites toward the edges, some toward the center, and sequences of active regulatory elements in that cell type (5). -
The Invected Gene of Drosophila: Sequence Analysis and Expression Studies Reveal a Close Kinship to the Engrailed Gene
Downloaded from genesdev.cshlp.org on September 30, 2021 - Published by Cold Spring Harbor Laboratory Press The invected gene of Drosophila: sequence analysis and expression studies reveal a close kinship to the engrailed gene Kevin G. Coleman, Stephen J. Poole, Michael P. Weir, Walter C. Soeller, and Thomas Kornberg Department of Biochemistry, University of Califomia at San Francisco, San Francisco, Califomia 94143 USA The invected and engrailed genes are juxtaposed in the Drosophila genome and are closely related in sequence and pattern of expression. The structure of the most abundant invected transcript was defined by obtaining the full-length cDNA sequence and by S1 nuclease sensitivity and primer extension studies; a partial sequence of the invected gene was determined; and the developmental profile of invected expression was characterized by Northern analysis and by in situ localization. The invected gene, like the engrailed gene, is expressed in the embryonic and larval cells of the posterior developmental compartments and in the embryonic hindgut, clypeolabrum, and nervous system. Like the engrailed gene, the invected gene can encode a protein of approximately 60 kD that contains a homeo box near its carboxyl terminus; indeed, a sequence of 117 amino acids in the carboxy-terminal region of both proteins is almost identical. The developmental role of the invected gene is not known. [Key Words: Drosophila; invected gene; gene expression; sequence analysis; homeo box] Received November 3, 1986; accepted November 25, 1986. There are few examples of eukaryotic genes that are the Antennapedia and Bithorax Complex genes (Poole et both functionally related and are organized as a linked al. -
ZEB2, the Mowat-Wilson Syndrome Transcription Factor: Confirmations, Novel Functions, and Continuing Surprises
G C A T T A C G G C A T genes Review ZEB2, the Mowat-Wilson Syndrome Transcription Factor: Confirmations, Novel Functions, and Continuing Surprises Judith C. Birkhoff 1, Danny Huylebroeck 1,2 and Andrea Conidi 1,* 1 Department of Cell Biology, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands; [email protected] (J.C.B.); [email protected] (D.H.) 2 Department of Development and Regeneration, Unit Stem Cell and Developmental Biology, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium * Correspondence: [email protected]; Tel.: +31-10-7043169; Fax: +31-10-7044743 Abstract: After its publication in 1999 as a DNA-binding and SMAD-binding transcription factor (TF) that co-determines cell fate in amphibian embryos, ZEB2 was from 2003 studied by embryologists mainly by documenting the consequences of conditional, cell-type specific Zeb2 knockout (cKO) in mice. In between, it was further identified as causal gene causing Mowat-Wilson Syndrome (MOWS) and novel regulator of epithelial–mesenchymal transition (EMT). ZEB2’s functions and action mechanisms in mouse embryos were first addressed in its main sites of expression, with focus on those that helped to explain neurodevelopmental and neural crest defects seen in MOWS patients. By doing so, ZEB2 was identified in the forebrain as the first TF that determined timing of neuro- /gliogenesis, and thereby also the extent of different layers of the cortex, in a cell non-autonomous fashion, i.e., by its cell-intrinsic control within neurons of neuron-to-progenitor paracrine signaling. Transcriptomics-based phenotyping of Zeb2 mutant mouse cells have identified large sets of intact- ZEB2 dependent genes, and the cKO approaches also moved to post-natal brain development Citation: Birkhoff, J.C.; Huylebroeck, and diverse other systems in adult mice, including hematopoiesis and various cell types of the D.; Conidi, A. -
Europe PMC Funders Group Author Manuscript Dev Genes Evol
Europe PMC Funders Group Author Manuscript Dev Genes Evol. Author manuscript; available in PMC 2014 July 01. Published in final edited form as: Dev Genes Evol. 2013 July ; 223(4): 237–246. doi:10.1007/s00427-013-0442-z. Europe PMC Funders Author Manuscripts The expression pattern of the genes engrailed, pax6, otd and six3 with special respect to head and eye development in Euperipatoides kanangrensis Reid 1996 (Onychophora: Peripatopsidae) Bo Joakim Eriksson1, Leyli Samadi, and Axel Schmid Department of Neurobiology, University of Vienna, Althanstrasse 14, 1090 Wien, Austria Abstract The genes otd/otx, six3, pax6 and engrailed are involved in eye patterning in many animals. Here we describe the expression pattern of the homologs to otd/otx, six3, pax6 and engrailed in the developing Euperipatoides kanangrensis embryos. Special reference is given to the expression in the protocerebral/ocular region. E.kanangrensis otd is expressed in the posterior part of the protocerebral/ocular segment before, during, and after eye invagination. E.kanangrensis otd is also expressed segmentally in the developing ventral nerve cord. The E.kanangrensis six3 is located at the extreme anterior part of the protocerebral/ocular segment and not at the location of the developing eyes. Pax6 is expressed in a broad zone at the posterior part of the protocerebral/ocular segment but only week expression can be seen at early onset of eye invagination. In late stages of development, the expression in the eye is upregulated. Pax6 is also expressed in the invaginating hypocerebral organs, thus supporting earlier suggestions that the hypocerebral organs in onychophorans are glands. -
The Drosophila Engrailed Gene
Downloaded from genesdev.cshlp.org on October 7, 2021 - Published by Cold Spring Harbor Laboratory Press Spatial and temporal control elements of the Drosophila engrailed gene Judith A. Kassis Laboratory of Cellular and Molecular Biology, Division of Biochemistry and Biophysics, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892 USA engrailed (en) is a segmentation gene expressed in a series of stripes throughout embryonic development. Here, I show that regulatory sequences for striped expression are present within the first intron of en. The 1-kb intron is able to confer striped expression early, but not late, in development. This shows that different regulatory sequences are required for en stripes at different times in development. Furthermore, stripes generated by the intron are coincident with en stripes in a wild-type background but behave differently from endogenous engrailed stripes in some segmentation mutant backgrounds. Thus, although the intron can induce apparently normal stripes, it lacks some of the regulatory sequences present within the endogenous gene. These experiments suggest that multiple regulatory programs control en expression in stripes, and each may be able to confer "normal" spatial regulation independently. [Key Words: Regulatory sequences; Drosophila engrailed gene] Received August 11, 1989; revised version accepted January 3, 1990. The engrailed (en) gene is 1 of >20 zygotic genes impor- To identify important sequences of the en gene, I have tant for segmentation of the Drosophila embryo (Jfirgens been studying its sequence conservation between two et al. 1984; Niisslein-Volhard et al. 1984; Wieschaus et distantly related Drosophila species, D. virilis and D.