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Angela N. Koehler Angela N. Koehler Broad Institute of MIT and Harvard Office: 617-714-7364 Chemical Biology Program Fax: 617-714-8943 7 Cambridge Center Email: [email protected] Cambridge, MA 02142 Website: www.broadinstitute.org/node/2445 Education Ph.D. Chemistry, Harvard University, 2003 B.A. Biochemistry and Molecular Biology, Reed College, 1997 Research Experience Investigator, Chemical Biology Program, Broad Institute 2009-present Project & Center Manager, Broad NCI Cancer Target Discovery and Development (CTD2) Center 2010-2012 Institute Fellow, Chemical Biology Program, Broad Institute 2003-2009 Director, Ligand Discovery, NCI Initiative for Chemical Genetics (ICG) at Harvard 2003-2009 Graduate Student, Department of Chemistry and Chemical Biology, Harvard University 1998-2003 Laboratory of Professor Stuart L. Schreiber Thesis: Small molecule microarrays: A high-throughput tool for discovering protein-small molecule interactions Researcher, Department of Chemistry, California Institute of Technology 1997-1998 Laboratory of Professor Barbara Imperiali Project: Biochemical reconstitution of the oligosaccharyltransferase (OST) complex Undergraduate Researcher, Department of Chemistry, Reed College 1995-1997 Laboratory of Professor Arthur Glasfeld Co-mentored by Professor Richard G. Brennan at Oregon Health Sciences University Thesis: Biochemical and structural characterization of the tRNA-modifying enzyme QueA from Escherichia coli Teaching Experience Research Advisor to postdoctoral fellows, undergraduates, high school students, and staff scientists 2003-present Broad Institute of MIT and Harvard Instructor, Biochemical Sciences Research (BS91r) 2004-2006, 2010-2011 Harvard University Associated Instructor, Experimental Research in the Life Sciences (LS100r) 2009 Harvard University Associated Instructor, Experimental Molecular and Cellular Biology (MCB100) 2004-2006 Harvard University Teaching Fellow, Chemical Biology (Chem 170) 1999, 2000 Harvard University, Professor David R. Liu Teaching Fellow, The Organic Chemistry of Life (Chem 27) 1999 Harvard University, Professor Stuart L. Schreiber Teaching Assistant, Techniques of Experimental Chemistry 1997-1998 Harvard University, Dr. Jane Raymond Teaching Assistant, Biochemical Methods Laboratory 1997 Reed College, Professor Arthur Glasfeld Teaching Assistant, General Chemistry Laboratory 1995-1997 Reed College, Professor Ronald W. McClard Professional Service Ad Hoc Member of NIH Drug Discovery and Molecular Pharmacology (DMP) Study Section 2012 Ad Hoc Member of NIH Synthetic and Biological Chemistry B (SBCB) Study Section 2011 Advisory Panel, NRC Canada, Genomics and Health Initiative, BioChips Project 2008-2011 Editorial Board, Molecular Diversity (Springer) 2008-2011 Broad Institute Educational Outreach Program Admissions Advisor and Research Mentor 2007-present Intel National Science Talent Search Mentor (Megan Blewett, 7th-place out of 1,700) 2006-2007 HTS Review Board, Broad Institute 2005-present Chembank Steering Committee, NCI Initiative for Chemical Genetics 2005-2006 Technology Platform Committee, Broad Institute 2004-2006 Honors and Awards Indo-US Frontiers of Science Exchange Award 2011 Kavli Frontiers of Science Scholar, US National Academy of Sciences 2011 Sigma Xi, MIT Chapter 2007-present Eli Lilly Predoctoral Fellowship in Organic Chemistry, Harvard University 2001-2002 MIT 50K Entrepreneur Competition, Finalist 2001 Excellence in Scholarship Award, Reed College 1995-1997 William and Jane Einzig Memorial Scholarship, Reed College 1993-1997 ESCO Undergraduate Scholarship, Reed College 1993-1997 Publications Broad Institute 1. Izhack Cherny, Maria Korolev, Angela N. Koehler, Michael H. Hecht. “Proteins from an unevolved library of de novo designed sequences bind a range of small molecules.” ACS Synth. Biol., 1, 130-138, 2012. 2. Melissa M. Kemp, Michel Weïwer, Angela N. Koehler.* “Unbiased binding assays for discovering small- molecule probes and drugs.” Bioorg. Med. Chem., 20, 1979-1989, 2012. 3. Dominick E. Casalena, Dina Wassaf, Angela N. Koehler.* “Ligand discovery using small-molecule microarrays.” Chapter, Methods Mol. Biol., 803, 249-263, 2012. 4. Melissa M. Kemp, Qiu Wang, Jason H. Fuller, Nathan West, Nicole Martinez, Elizabeth M. Morse, Michel Weïwer, Stuart L. Schreiber, James E. Bradner, Angela N. Koehler,* “A novel HDAC inhibitor with a hydroxy- pyrimidine scaffold.” Bioorg. Med. Chem. Lett., 21, 4164-4169, 2011. 5. Paul A. Clemons, J. Anthony Wilson, Vlado Dancik, Sandrine Muller, Hyman A. Carrinski, Bridget K. Wagner, Angela N. Koehler, Stuart L. Schreiber. “Quantifying structural property distributions and patterns of performance among sets of small molecules comprising small-molecule screening collections.” Proc. Natl. Acad. Sci. USA, 108, 6817-6822, 2011. 6. Myung-Soo Kang, Eun Kyung Lee, Vishal Soni, Timothy A. Lewis, Angela N. Koehler, Viswanathan Srinivasana, Elliott Kieff. ‘Roscovitine inhibits ENBA1 Serine 393 phosphorylation, nuclear localization, transcription, and episome maintenance.” J. Virol. 85, 2859-2868, 2011. 7. Jermont M. Chen, Anne H. Armstrong, Angela N. Koehler, Michael H. Hecht. “Small-molecule microarrays enable the discovery of compounds that bind the Alzheimer’s Aβ peptide and reduce its cytotoxicity.” J. Am. Chem. Soc., 132, 17015-17022, 2010. This article was featured in the Research Highlights section of Nature Chemistry, November 26 2010. 8. Paul A. Clemons, Nicole E. Bodycombe, Hyman A. Carrinski, J. Anthony Wilson, Alykhan F. Shamji. Bridget K. Wagner, Angela N. Koehler, Stuart L. Schreiber. “Small molecules of different synthetic and natural origins have distinct distributions of structural complexity that correlate with protein-binding profiles.” Proc. Natl. Acad. Sci. USA, 107, 18787-18792, 2010. 9. The Cancer Target Discovery and Development Network: Stuart L. Schreiber, Alykhan F. Shamji, Paul A. Clemons, Cindy Hon, Angela N. Koehler, Benito Munoz, Michelle Palmer, Andrew M. Stern, Bridget K. Wagner, Scott Powers, Scott W. Lowe, Xuecui Guo, Alex Krasnitz, Eric T. Sawey, Raffaella Sordella, Lincoln Stein, Lloyd C. Trotman, Andrea Califano, Riccardo Dalla-Favera, Adolfo Ferrando, Antonio Iavarone, Laura Pasqualucci, José Silva, Brent R. Stockwell, William C. Hahn, Lynda Chin, Ronald A. DePinho, Jesse S. Boehm, Shuba Gopal, Alan Huang, David E. Root, Barbara A. Weir, Daniela S. Gerhard, Jean Claude Zenklusen, Michael G. Roth, Michael A. White, John D. Minna, John B. MacMillan, Bruce A. Posner. “Towards patient-based cancer therapeutics.” Nat. Biotechnol. 28, 904-906, 2010. 10. Angela N. Koehler.* “A complex task? Direct modulation of transcription factors with small molecules.” Curr. Opin. Chem. Biol. 14, Curr. Opin. Chem. Biol., 14, 331-340, 2010. 11. Arturo J. Vegas, Angela N. Koehler.* “Detecting protein-small molecule interactions using fluorous small- molecule microarrays.” Methods Mol. Biol., 669, 43-55, 2010. 12. Carlos Tassa, Jay L. Duffner, Timothy A. Lewis, Ralph Weissleder, Stuart L. Schreiber, Angela N. Koehler, Stanley Y. Shaw. “Binding affinity and kinetic analysis of targeted small molecule-modified nanoparticles.” Bioconjugate Chemistry, 21, 14-19, 2010. 13. Benjamin Z. Stanton, Lee F. Peng, Nicole Maloof, Kazuo Nakai, Xiang Wang, Jay L. Duffner, Kennedy M. Taveras, Joel M. Hyman, Sam W. Lee, Angela N. Koehler, James K. Chen, Julia L. Fox, Anna Mandinova, Stuart L. Schreiber. “A small molecule that binds Hedgehog and blocks signaling in human cells.” Nature Chemical Biology, 5, 154-156, 2009. This article was featured in the Science & Technology Concentrates section of Chemical and Engineering News, 87, 4, 31, 2009. 14. Yingwei Mao, Xuecai Ge, Christopher L. Frank, Jon Madison, Angela N. Koehler, Mary K. Doud, Carlos Tassa, Erin M. Berry, Tracey L. Petryshen, Takahiro Soda, Travis Biechele, Randall T. Moon, Stephen J. Haggarty, Li- Huei Tsai. “DISC1 regulates neural progenitor proliferation via modulation of GSK3β/β-catenin signaling.” Cell, 136, 1017-1031, 2009. Cover image. 15. Shao-En Ong, Monica Schenone, Adam A. Margolin, Xiaoyu Li, Kathy Do, Mary K. Doud, Denkanikota R. Mani, Letian Kuai, Xiang Wang, John L. Wood, Nicola J. Tolliday, Angela N. Koehler, Lisa A. Marcaurelle, Todd R. Golub, Robert J. Gould, Stuart L. Schreiber, Steve A. Carr. “Identifying the proteins to which small-molecule probes and drugs bind in cells.” Proc. Natl. Acad. Sci. USA, 106, 4617-4622, 2009. 16. Angela N. Koehler.* “Microarrays in chemical biology.” Chapter, Wiley Encyclopedia of Chemical Biology, Tadhg Begley, Editor, ISBN: 978-0-471-75477-0, 2009. 17. Arturo J. Vegas, Jason H. Fuller, Angela N. Koehler.* “Small-molecule microarrays as tools in ligand discovery.” Chem. Soc. Rev., 37, 1385-1394, 2008. 18. Thomas J. F. Nieland, Jared T. Shaw, Firoz A. Jaipuri, Jay. L. Duffner, Angela N. Koehler, Sotirios Banakos, Vassilis I. Zannis, Tom Kirchhausen, Monty Krieger. “Identification of the molecular target for small molecule inhibitors of HDL receptor SR-BI activity.” Biochemistry, 47, 460-472, 2008. 19. Katja Schmitz, Stephen J. Haggarty, Olivia M. McPherson, Jon Clardy, Angela N. Koehler.* “Detecting binding interactions using microarrays of natural product extracts.” J. Am. Chem. Soc., 129, 11346-11347, 2007. 20. Arturo J. Vegas, James E. Bradner, Weiping Tang, Olivia M. McPherson, Edward F. Greenberg, Angela N. Koehler, Stuart L. Schreiber. “Fluorous-based small-molecule microarrays for the discovery of histone deacetylase inhibitors.” Angew. Chem. Int. Ed. 46, 7960-7964, 2007. This article was featured in
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