DNA PCR-assayed LC-MS/MS assayed major blood meal Additional peptides not matching major blood meal (s) suggesting: Sample ID blood meal sources sources 1) minor/low abundance blood meal; 2) previously unknown polymorphism; or 3) SEQUEST mis-identification of peptide sequence

VAAHVEEYGAETLER VLTALGEAVNHIDDLASALSK Jkop_001 TPG_007 chicken Testudines Testudines ISGHAEEYGAETLER KVLTALGEAVNHIDDLASALSK VLTALGEAVNHIDDLASALSK VAAHVEEYGAETLER Jkop_002 TPG_011 bird chicken/ turkey Testudines Testudines Testudines VGDALGNAVAHLDDLPGALSALSDLHAHK Jkop_003 TPG_012 bird chicken/ turkey mouse Chiroptera rodentia/ VNVDEVGAEALGR LSDLHAQK Jkop_007 TPG0044 dog dog human sciuridae Insectivora 30 Aves, 2 Reptilia orders ASGHLEAFGAEALER KVVAALIEAANHIDDIAGTLSK VNVDEVGAEALGR Jkop_008 TPG_067 pig human Capriulgiformes Rodentia Insectivora rodentia/ Jkop_009 TPG0075 dog dog human sciuridae MLVVYPWTQR AVGHLDDLPGTLSDLSDLHAHK Jkop_011 TPG_086 rat dog rat chicken Tubulidentata, Squamata Cetartiodactyla VLRAIGNAASHLDDISGALSALSDLHAYK VNVDEVGGEVLGR Jkop_012 TPG_087 pig dog human Anura Afrosoricida VLRAIGNAASHLDDISGALSALSDLHAYK VNVDEVGGEVLGR VNVEEIGGEALGR Jkop_013 TPG_088 dog dog Anura Afrosoricida Rodentia, Chiroptera, Cetacea VLTALGEAVNHIDDLASALSK VAAHVEEYGAETLER VADALTTAVAHLDDLPGALSALSDLHAYK Jkop_015 TPG_092 pig chicken/ turkey Testudines Testudines Carnivora VLTALGEAVNHIDDLASALSK VAAHVEEYGAETLER FFESFGDLSTPDAVMGNPK IGGHAGEYGAEALER ISGHAEEYGAETLER VADALTTAVAHLDDLPGALSALSDLHAYK VNVDEVGGEALGR VVAGVANALAHK Jkop_016 TPG0097 bird chicken/ turkey Carnivora, Primates, Cetartiodactyla, Testudines Testudines Primates Rodentia Anseriformes Carnivora Insectivora, Chiroptera 17 Mammal orders rodentia/ Jkop_017 TPG0100 dog dog human sciuridae TYFPHYDLSPGSAQVK VVAGVANALAHK VNVEEIGGEALGR Jkop_018 TPG101 dog dog human Insectivora Rodentia Rodentia, Chiroptera, Cetacea rodentia/ TYFPHYDLSPGSAQVK VGGHAGEYGAEALDR Jkop_019 TPG0104 dog dog human sciuridae Insectivora Lagomorpha VVAALIEAVNHIDDIAGTLSK Jkop_020 TPG0178 human human VLTALGEAVNHIDDLASALSK Jkop_021 TPG0158 bird chicken/ turkey Testudines ISGHADQYGAETLER VAAHVEEYGAETLER VADALTTAVAHLDDLPGALSALSDLHAYK Jkop_022 TPG0168 opossum 12S cow chicken/ turkey Anseriformes Testudines Carnivora

Jkop_023 TPG186 bird opossum turkey/ chicken

Rattus Jkop_024 TPG0238 rat norvegicus

Jkop_025 TPG0336 bird dog dog turkey/ chicken human

ISGHAEEYGAETLER KVLTALGEAVNHIDDLASALSK VLTALGEAVNHIDDLASALSK VAAHVEEYGAETLER FFSSFGNLSSPTAILGNPMVR Jkop_027 TPG0630 bird human chicken/ turkey Anseriformes Testudines Testudines Testudines Anseriformes, Struthioniformes, Ciconiiformes 6 canidae ISGHADQYGAETLER Jkop_028 TPG_670 mouse turkey/ chicken species Opisthocomiformes turkey/chicken/ Jkop_006 TPG0038 12S human francolin VLTALGEAVNHIDDLASALSK VAAHVEEYGAETLER Jkop_004 TPG0028 turkey/ chicken Testudines Testudines VAAHVEEYGAETLER VLSADDKANVK Jkop_005 TPG0030 turkey/ chicken human dog Testudines Rodentia ASGHLEAFGAEALER IGGHAGEYGAEALER LRVDPVDFK VNVDEVGGEVLGR FFESFGDLSNADAVMGNPK VNVDEVGGEALGR Jkop_029 TPG_016 human Carnivora, Primates, Cetartiodactyla, Insectivora, Caprimulgiformes Rodentia Cetartiodactyla Afrosoricida Cetartiodactyla Chiroptera FLASVSTVLTSK VVAGVANALAHK FFASFGNLSSPTAITGNPMVR Jkop_030 TPG0045 2 species 10 Mammal orders 17 Mammal orders Galliformes LRVDPVDFK VNVDEVGGEVLGR VVAGVANALSHK VNVEEIGGEALGR Jkop_031 TPG0102 dog human Cetartiodactyla Afrosoricida Rodentia Rodentia, Chiroptera, Cetacea IGGHAGEYGAEALER Jkop_032 TPG0159 chicken human Rodentia

Jkop_033 TPG0244 human

VVAALIEAANHIDDIAGTLSK FFASFGNLSSPTAILGNPMVR Jkop_034 TPG0263 human opossum Galliformes 14 Aves orders

Jkop_035 TPG0288 10 species *

IGGHAGEYGAEALER KVVAALIEAANHIDDIAGTLSK LLGDILIIVLAAHFSK FFASFGNLSSPTAILGNPMVR Jkop_036 TPG0302 dog human Rodentia Gallformes 10 Aves orders 14 Aves orders IGGHAGEYGAEALER Jkop_037 TPG0334 dog chicken/ turkey human Rodentia Jkop_038 TPG0381 chicken/ turkey

ASGHLEAFGAEALER NVADEVGGEALGR YFDSFGDLSSASAIMGNAK IAGHAEEYGAETLER FFASFGNLSSPTAILGNPMVR Jkop_039 TPG0385 human Caprimulgiformes Rodentia Rodentia Galliformes 14 Aves orders ASGHLEAFGAEALER VNVDEVGGEVLGR VNVEEIGGEALGR Jkop_040 TPG0412 human Caprimulgiformes Afrosoricida Rodentia, Chiroptera, Cetacea rodentia/ FFASFGNLSSPTAILGNPMVR VVAALIEAANHIDDIAGTLSK Jkop_041 TPG0415 human cricetidae 14 Aves orders Galliformes ASGHLEAFGAEALER VNVEEIGGEALGR Jkop_042 TPG0471 human Caprimulgiformes Rodentia, Chiroptera, Cetacea IGGHAGEYGAEALER VAAHVEEYGAETLER VNVDEVGGEVLGR Jkop_043 TPG0585 human chicken Rodentia Testudines Afrosoricida IGGHAGEYGAEALER VNVEEIGGEALGR Jkop_044 TPG0596 human bovidae Rodentia Rodentia, Chiroptera, Cetacea rodentia/ VLRAIGNAASHLDDISGALSALSDLHAYK TYFPHYDLSPGSAQVK Jkop_045 TPG0603 dog human sciuridae Anura Insectivora IAGHAEEYGAETLER VVAALIEAANHIDDIAGTLSK VGSHAGEYGAEALER VNVEEIGGEALGR FFASFGNLSSPTAILGNPMVR Jkop_046 TPG0635 human Galliformes Galliformes Rodentia, Perissodactyla, Lagomorpha Rodentia, Chiroptera, Cetacea 14 Aves orders DNA PCR-assayed LC-MS/MS assayed major blood meal Additional peptides not matching major blood meal order(s) suggesting: Sample ID blood meal sources sources 1) minor/low abundance blood meal; 2) previously unknown polymorphism; or 3) SEQUEST mis-identification of peptide sequence

VAAALVEAVNHIDDIAGALSK Jkop_047 TPG0679 turkey/ chicken Anseriformes, Strigiformes, Passeriformes Jkop_048 TPG0703 human

VNVEEIGGEALGR Jkop_049 TPG0749 dog human Rodentia, Chiroptera, Cetacea Jkop_050 TPG0771 human dog

Jkop_051 TPG0807 pig rodent

Jkop_052 TPG0814 106 species *

LTGAVAHALAR VAAHVEEYGAETLER Jkop_053 TPG0717 chicken/ turkey Squamata Testudines