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Analysis of propolis Monitoring recommendations

High quality propolis should be tested for common quality parameters, in particular the content of polyphenols.

Furthermore, as a natural product with a highly variable composition, propolis also has to be free of any antibiotic residues derived from practices and from contaminants caused by pollution. Raw propolis is known to accumulate considerable amounts of pesticides as well as heavy metals, especially Lead.

How can you set up an effective control strategy for risk based monitoring? Propolis is a natural bee product with a highly variable composition, especially with regards to the polyphenols it contains. Significant differences between propolis can be observed depending on its botanical and geographical origin. Intertek is a world-leading expert in the analysis of and hive products. We are proud to offer our customers a tailored service, with practical advice and fast, reliable test results.

Our Service Intertek’s comprehensive service includes an the Hyperoside method according to the European extended pesticide analysis which incorporates Pharmacopoeia. We can implement certain other the Liquid Chromatography - Mass Spectronomy polyphenols upon request. (LC-MS/MS) technique and currently covers over Antibiotics: 400 substances. Our recommended analysis for • Chloramphenicol by LC-MS/MS (prohibited raw propolis and propolis products (dry and substance in the EU) alcoholic liquid extracts) includes: • Sulfonamides and Trimethoprim by LC-MS/ Quality parameters: MS • Total polyphenols by Folin-Ciocalteu method • Tetracyclines by LC-MS/MS (calibration against a mixture of pinocembrin and galangin). The polyphenol content should • Streptomycin by LC-MS/MS exceed certain amounts depending on the Pesticides: origin of the propolis • Multi-residue analysis by Gas Chromatography- • Wax content (Soxhlet extraction) Mass Spectronomy (GC-MS(/MS)) and LC-MS/ • Ash content MS • Specific flavonoids and phenolic acids by • Or a selection of pesticide classes (e.g. High Performance Liquid Chromatography- Organochlorines, Organophosphates and Ultra Violet (HPLC-UV) Diode array: the Pyrethroids by GC-MS(/MS) determination of 16 common polyphenols Contaminants in propolis, e.g. pinocembrin, galangin and • Heavy metals (e.g. Lead) as the main flavonoids in Poplar-type Other parameters upon request: propolis • Microbiology (e.g. according to the European We also offer the determination of flavonoids Pharmacopoeia) using other photometric methods. These include the Quercetine method with aluminium chloride or

Benefiting You • We are the experts in the analysis of honey and • Our specialists will support you in developing Your Intertek Food Services hive products, including propolis individual risk-based monitoring plans for Regional Contacts: • You receive a tailored service, with practical your products, including guidance on which parameters should be analysed and at what Europe advice and fast, reliable test results intervals Intertek Food Services • We test approximately 60,000 honey samples Olof-Palme-Str. 8 per year for quality parameters, veterinary drugs • You will benefit from our wealth of experience, 28719 Bremen and/or authenticity. This information is added enabling you to minimise your risks and Germany to a comprehensive database which is used for optimise your cost to benefit ratio Tel: +49 421 65727 390 providing specific analytical recommendations • We can also advise on legislation where required [email protected] to our customers

Asia With You All The Way • Risk Management: Improve performance, Intertek Food Services Propolis testing and analysis is just one of a suite reduce risk and free up valuable resource Global Customer Service Centre of services we provide to ensure the safety and • Global Quality Guarantee: applied to 80 Bendemeer Road quality of your products. commodities, food ingredients and food Hyflux Innovation Centre products #03-02 Intertek is one of the world’s leading food safety Singapore 339949 testing, auditing, inspection and certification • Traceability through the supply chains: Tel: +65 68 05 48 88 bodies. Our blend of expert analytical and advisory including raw and semi-manufactured [email protected] services help our customers to achieve compliance foodstuffs and final products in all food and improve safety, quality and efficiency. categories and special interest products

Americas Whatever safety or quality issues you face, we • Global Coverage: network of highly skilled Intertek Food Services have the flexibility and the experience to deliver and experienced experts to ensure smooth 711 Parkland Court the right solutions for your business. transition in the supply chain. Champaign Illinois, 61821, United States About Intertek Tel: 1 844 818 6624 [email protected] At Intertek, it is our sole mision to provide not only the one-stop-shop you seek for all food services but to establish a trusted partnership with your business - consistently adding value and increasing your www.intertek.com/food competitive advantage in the industry with every step we take together. 131016ENG_JT www.intertek.com/food