Listeria Monocytogenes Virulence Factors
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Ankara Üniversitesi Fen Bilimleri Enstitüsü Yüksek
ANKARA ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ YÜKSEK LİSANS TEZİ ET VE ET ÜRÜNLERİNİN Listeria spp. VARLIĞI BAKIMINDAN ARAŞTIRILMASI Raşit KESKİN GIDA MÜHENDİSLİĞİ ANABİLİM DALI ANKARA 2020 Her hakkı saklıdır ÖZET Yüksek Lisans Tezi ET VE ET ÜRÜNLERİNİN Listeria spp. VARLIĞI BAKIMINDAN ARAŞTIRILMASI Raşit KESKİN Ankara Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı Danışman: Doç. Dr. Pınar ŞANLIBABA Bu çalışmada Ankara’daki farklı satış yerlerinden temin edilen birbirinden farklı çiğ ve işlem görmüş et ürünlerinin Listeria spp. varlığı bakımından taranması amaçlanmıştır. 116 gıda örneğinin taraması, ISO 11290-1 protokolü doğrultusunda yapılmıştır. Gram (+), katalaz (+), oksidaz (-) ve hareketlilik (+) sonuç veren 40 izolatın, tür düzeyinde biyokimyasal olarak tanımlamasında API® Listeria test kiti kullanılmıştır. Bu kapsamda, 40 izolattan 15’i L. monocytogenes, 12’si L. innocua, 8’i L. welshimeri ve 4’ü ise L. grayi olarak tanımlanmıştır. 1 izolatın tanımlaması yapılamamıştır. Listeria spp. ve L. monocytogenes varlığı bakımından kıyma en riskli gıda olarak saptanmıştır. Çiğ etin %67,50’si ve işlem görmüş etin ise %32,50’si Listeria spp. ile kontamine olmuştur. Kırmızı et ve et ürünlerinin, beyaz et ve et ürünlerine oranla daha riskli olduğu saptanmıştır. Ankara’da satışa sunulan et ve et ürünleri, L. monocytogenes bakımından riskli bulunmuştur. 2020, 73 sayfa Anahtar Kelimeler: Listeria, et ve et ürünleri, izolasyon, biyokimyasal tanımlama iv ABSTRACT Master Thesis AN INVESTIGATION ON THE PRESENCE OF Listeria spp. IN MEAT AND MEAT PRODUCTS Raşit KESKİN Ankara University Graduate School of Natural and Applied Sciences Department of Food Engineering Supervisor: Assoc. Prof. Dr. Pınar ŞANLIBABA In this study, it was aimed to screen different raw and processed meat products obtained from different markets in Ankara for Listeria spp. -
Nama from Listeria Monocytogenes
Identification and Characterisation of an Old Yellow Enzyme (OYE) - NamA from Listeria monocytogenes by Majid Abdullah Bamaga A thesis submitted in partial fulfilment of the requirements of Edinburgh Napier University, for the award of Doctor of Philosophy September, 2014 Abstract The food-borne pathogene Listeria monocytogenes has been considered a significant threat to human health worldwide. It mainly infects individuals suffering insuffecint immunity such as pregnant women. During pregnancy, L. monocytogenes is capable of causing a serious damage to the mother and the fetus. It can spread to different organs including the placenta via adaptation to interacellular lifestyle. To maintain pregnancy, the levels of the hormones progesterone and β-estradiol increase and reduction in hormone levels was proposed to be associated with fetal death and abortion. The objectives of this project therefore were to investigate the role of pregnancy hormones on the growth and virulence of L. monocytogenes, and to identify bacterial genes with possible roles in binding to pregnancy hormones. It was obsereved that the growth of L. monocytogenes in the presence of progesterone under anaerobic condition was affected by the action of the hormone and the effect was dose/time-dependent of exposure as increasing concentrations showed greater effect on the bacterial growth. Interestingly, bacterial growth was restored within 24 h of exposure to the hormone. In parallel, a Tn917-LTV3 insertion library was constructed and a number of mutants isolated that had reduced growth in the presence of β-estradiol were identified. However, reduction in growth was not microbiologically significant. Furthermore, bioinformatics analysis was performed to identify listerial genes with possible role in hormones degradation. -
Cross-Resistance to Phage Infection in Listeria Monocytogenes Serotype 1/2A Mutants and Preliminary Analysis of Their Wall Teichoic Acids
University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Masters Theses Graduate School 8-2019 Cross-resistance to Phage Infection in Listeria monocytogenes Serotype 1/2a Mutants and Preliminary Analysis of their Wall Teichoic Acids Danielle Marie Trudelle University of Tennessee, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_gradthes Recommended Citation Trudelle, Danielle Marie, "Cross-resistance to Phage Infection in Listeria monocytogenes Serotype 1/2a Mutants and Preliminary Analysis of their Wall Teichoic Acids. " Master's Thesis, University of Tennessee, 2019. https://trace.tennessee.edu/utk_gradthes/5512 This Thesis is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Masters Theses by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a thesis written by Danielle Marie Trudelle entitled "Cross-resistance to Phage Infection in Listeria monocytogenes Serotype 1/2a Mutants and Preliminary Analysis of their Wall Teichoic Acids." I have examined the final electronic copy of this thesis for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Master of Science, with a major in Food Science. Thomas G. Denes, Major Professor We have read this thesis and recommend its acceptance: -
Thesis Listeria Monocytogenes and Other
THESIS LISTERIA MONOCYTOGENES AND OTHER LISTERIA SPECIES IN SMALL AND VERY SMALL READY-TO-EAT MEAT PROCESSING PLANTS Submitted by Shanna K. Williams Department of Animal Sciences In partial fulfillment of the requirements for the degree of Master of Science Colorado State University Fort Collins, Colorado Fall 2010 Master’s Committee: Department Chair: William Wailes Advisor: Kendra Nightingale John N. Sofos Doreene Hyatt ABSTRACT OF THESIS DETECTION AND MOLECULAR CHARACTERIZATION OF LISTERIA MONOCYTOGENES AND OTHER LISTERIA SPECIES IN THE PROCESSING PLANT ENVIRONMENT Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne disease associated with a high case fatality rate. To prevent product contamination with L. monocytogenes, it is crucial to understand Listeria contamination patterns in the food processing plant environment. The aim of this study was to monitor Listeria contamination patterns for two years in six small or very small ready-to-eat (RTE) meat processing plants using a routine combined cultural and molecular typing program. Each of the six plants enrolled in the study were visited on a bi-monthly basis for a two-year period where samples were collected, microbiologically analyzed for Listeria and isolates from positive samples were characterized by molecular subtyping. Year one of the project focused only on non-food contact environmental samples within each plant, and year two focused again on non-food contact environmental samples as well as food contact surfaces and finished RTE meat product samples from participating plants. Between year one and year two of sampling, we conducted an in-plant training session ii involving all employees at each plant. -
Stress Tolerance of Listeria Monocytogenes and Control of the Bacterium in the Fish Industry
Department of Food Hygiene and Environmental Health Faculty of Veterinary Medicine University of Helsinki Finland Stress tolerance of Listeria monocytogenes and control of the bacterium in the fish industry Mariella Aalto-Araneda DOCTORAL DISSERTATION To be presented for public discussion with the permission of the Faculty of Veterinary Medicine of the University of Helsinki, in Lecture Room 107, Athena (Siltavuorenpenger 3 A, Helsinki), on the 25th of March, 2020 at 12 o’clock. Helsinki 2020 Supervising Professor Professor Miia Lindström, DVM, PhD Department of Food Hygiene and Environmental Health Faculty of Veterinary Medicine University of Helsinki Helsinki, Finland Supervisors Professor Emeritus Hannu Korkeala, DVM, PhD Department of Food Hygiene and Environmental Health Faculty of Veterinary Medicine University of Helsinki Helsinki, Finland Associate Professor Janne Lundén, DVM, PhD Department of Food Hygiene and Environmental Health Faculty of Veterinary Medicine University of Helsinki Helsinki, Finland Reviewers Professor Lisbeth Truelstrup Hansen, PhD National Food Institute Technical University of Denmark Lyngby, Denmark Professor Martin Wiedmann, DVM, PhD Department of Food Science College of Agriculture and Life Sciences Cornell University Ithaca, NY, USA Opponent Docent Jaana Husu-Kallio, DVM, PhD Ministry of Agriculture and Forestry Helsinki, Finland ISBN 978-951-51-5840-6 (pbk.) ISBN 978-951-51-5841-3 (PDF) Unigrafia Helsinki 2020 Food safety does not happen by accident Christopher James Griffith, 2010 ABSTRACT The severe foodborne disease listeriosis is caused by the bacterium Listeria monocytogenes, known as a problematic contaminant of the food chain. This facultative anerobe tolerates many conditions used for controlling harmful bacteria, including high salinity and temperature. Some L. -
Listeria Costaricensis Sp. Nov. Kattia Núñez-Montero, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Johnny Peraza, Javier Pizarro-Cerdá, Marc Lecuit
Listeria costaricensis sp. nov. Kattia Núñez-Montero, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Johnny Peraza, Javier Pizarro-Cerdá, Marc Lecuit To cite this version: Kattia Núñez-Montero, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Johnny Peraza, et al.. Listeria costaricensis sp. nov.. International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2018, 68 (3), pp.844-850. 10.1099/ijsem.0.002596. pasteur-02320001 HAL Id: pasteur-02320001 https://hal-pasteur.archives-ouvertes.fr/pasteur-02320001 Submitted on 18 Oct 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives| 4.0 International License Listeria costaricensis sp. nov. Kattia Núñez-Montero1,*, Alexandre Leclercq2,3,4*, Alexandra Moura2,3,4*, Guillaume Vales2,3,4, Johnny Peraza1, Javier Pizarro-Cerdá5,6,7#, Marc Lecuit2,3,4,8# 1 Centro de Investigación en Biotecnología, Escuela de Biología, Instituto Tecnológico de Costa Rica, Cartago, Costa Rica 2 Institut Pasteur, -
Listeria Monocytogenes Numa Queijaria
Estudo das vias de contaminação de Listeria monocytogenes numa queijaria PFGE, serotipagem, WGS e resistência a desinfetantes Mestrado em Inovação e Qualidade na Produção Alimentar Ana Rita Simões Ferraz Orientadores Cristina Maria Baptista Santos Pintado Carla Maria Heliodoro Maia Relatório de estágio apresentado à Escola Superior Agrária do Instituto Politécnico de Castelo Branco e ao Instituto Nacional de Saúde Doutor Ricardo Jorge para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Inovação e Qualidade na Produção Alimentar, realizada sob a orientação científica da Doutora Cristina Maria Baptista Santos Pintado, Professora Adjunta da Escola Superior Agrária do Instituto Politécnico de Castelo Branco e da Mestre Carla Maria Heliodoro Maia Técnica Superior do Laboratório de Microbiologia, Departamento de Alimentação e Nutrição do Instituto Nacional de Saúde Doutor Ricardo Jorge, I.P.-Lisboa Junho 2017 II Composição do júri Presidente do júri Doutor, Celestino António Morais de Almeida, Professor Coordenador da Escola Superior Agrária de Castelo Branco Vogais Orientadores: Doutora, Cristina Maria Baptista Santos Pintado, Professora Adjunta da Escola Superior Agrária de Castelo Branco. Mestre, Carla Maria Heliodoro Maia, Técnica Superior no Instituto Nacional de Saúde Doutor Ricardo Jorge. Arguentes: Doutor, João Pedro Martins da Luz, Professor Coordenador da Escola Superior Agrária de Castelo Branco. Doutor, Manuel Vicente de Freitas Martins, Professor Coordenador da Escola Superior Agrária de Castelo Branco. III IV Dedicatória Eu, Ana Rita Simões Ferraz dedico o presente trabalho aos meus pais por todo o trabalho, dedicação, carinho e amor com que me educaram e fizeram de mim a pessoa que sou hoje. Em especial à minha mãe, por ser a enorme Mulher que é, que me faz crescer e lutar pelos meus sonhos. -
Properties of the Extracellular Polymeric Substance Layer from Minimally Grown Planktonic Cells of Listeria Monocytogenes
biomolecules Article Properties of the Extracellular Polymeric Substance Layer from Minimally Grown Planktonic Cells of Listeria monocytogenes Ogueri Nwaiwu 1,*, Lawrence Wong 1,2, Mita Lad 1, Timothy Foster 1, William MacNaughtan 1 and Catherine Rees 1 1 Division of Food, Nutrition and Dietetics, School of Biosciences, University of Nottingham, Nottingham LE12 5RD, UK; [email protected] (L.W.); [email protected] (M.L.); [email protected] (T.F.); [email protected] (W.M.); [email protected] (C.R.) 2 Senthink Science & Technology, Hangzhou 310000, China * Correspondence: [email protected] Abstract: The bacterium Listeria monocytogenes is a serious concern to food processing facilities because of its persistence. When liquid cultures of L. monocytogenes were prepared in defined media, it was noted that planktonic cells rapidly dropped out of suspension. Zeta potential and hydrophobicity assays found that the cells were more negatively charged (−22, −18, −10 mV in defined media D10, MCDB 202 and brain heart infusion [BHI] media, respectively) and were also more hydrophobic. A SEM analysis detected a capsular-like structure on the surface of cells grown in D10 media. A crude extract of the extracellular polymeric substance (EPS) was found to contain cell-associated proteins. The proteins were removed with pronase treatment. The remaining non- proteinaceous component was not stained by Coomassie blue dye and a further chemical analysis of the EPS did not detect significant amounts of sugars, DNA, polyglutamic acid or any other Citation: Nwaiwu, O.; Wong, L.; specific amino acid. When the purified EPS was subjected to attenuated total reflectance-Fourier Lad, M.; Foster, T.; MacNaughtan, W.; Rees, C. -
A Critical Review on Listeria Monocytogenes
International Journal of Innovations in Biological and Chemical Sciences, Volume 13, 2020, 95-103 A Critical Review on Listeria monocytogenes Vedavati Goudar and Nagalambika Prasad* *Department of Microbiology, Faculty of Life Science, School of Life Sciences, JSS Academy of Higher Education & Research, Mysuru, Karnataka, Pin code: 570015, India ABSTRACT Listeria monocytogenes is an omnipresent gram +ve, rod shaped, facultative, and motile bacteria. It is an opportunistic intracellular pathogenic microorganism that has become crucial reason for human food borne infections worldwide. It causes Listeriosis, the disease that can be serious and fatal to human and animals. Listeria outbreaks are often linked to dairy products, raw vegetables, raw meat and smoked fish, raw milk. The most effected country by Listeriosis is United States. CDC estimated that 1600 people get Listeriosis annually and regarding 260 die. It additionally contributes to negative economic impact because of the value of surveillance, investigation, treatment and prevention of sickness. The analysis of food products for presence of pathogenic microorganisms is one among the fundamental steps to regulate safety of food. This article intends to review the status of its introduction, characteristics, outbreaks, symptoms, prevention and treatment, more importantly to controlling the Listeriosis and its safety measures. Keywords: Listeria monocytogenes, Listeriosis, Food borne pathogens, Contamination INTRODUCTION Food borne health problem is outlined by the World Health Organization as “diseases, generally occurs by either infectious or hepatotoxic in nature, caused by the agents that enter the body through the activity of food WHO 2015 [1]. Causes of food borne health problem include bacteria, parasites, viruses, toxins, metals, and prions [2]. -
Bacillus Spp
Characterizing the culturable bacteria isolated from imported, ready-to-eat (RTE) foods for their ability to control Listeria monocytogenes by Krishna Sen Gelda A Thesis presented to The University of Guelph In partial fulfilment of requirements for the degree of Master of Science in Food Science Guelph, Ontario, Canada © Krishna Sen Gelda, September 2019 ABSTRACT CHARACTERIZING THE CULTURABLE BACTERIA ISOLATED FROM IMPORTED, READY-TO-EAT (RTE) FOODS FOR THEIR ABILITY TO CONTROL LISTERIA MONOCYTOGENES Krishna Sen Gelda Advisor: University of Guelph, 2019 Dr. Jeff Farber Listeria monocytogenes, an important foodborne pathogen, remains a significant threat to public health. This thesis investigated the culturable microbiota of select imported, RTE foods to see whether the existing bacterial microflora could inactivate, inhibit the growth and/or cause a reduction in the virulence of L. monocytogenes. Among all the foods tested (dried apple slices, cumin seeds, date fruits, fennel seeds, pistachios, pollen, raisins and seaweed), the date fruit microbiota displayed the most promise for harbouring antagonistic properties against L. monocytogenes. Of the 191 isolates recovered from five different date fruits, 36 (19%) produced zones of inhibition against L. monocytogenes that ranged from 0.3 to 5.8 mm. The inhibitory strains were all identified as Bacillus spp. Among those Bacillus spp. that were tested for their ability to inhibit PrfA, all caused a significant reduction in the activation of the PrfA protein (p-value < 0.05). In addition, the anti-Listeria compound(s) produced by B. altitudinis DS11 were found to be proteinaceous in nature, acid and alkali-tolerant and resistant to temperature treatments up to 100oC. -
Beno Cornellgrad 0058F 10677.Pdf
BACILLALES INFLUENCE QUALITY AND SAFETY OF DAIRY PRODUCTS A Dissertation Presented to the Faculty of the Graduate School of Cornell University In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy by Sarah Marie Beno December 2017 © 2017 Sarah Marie Beno BACILLALES INFLUENCE QUALITY AND SAFETY OF DAIRY PRODUCTS Sarah Marie Beno, Ph. D. Cornell University 2017 Bacillales, an order of Gram-positive bacteria, are commonly isolated from dairy foods and at various points along the dairy value chain. Three families of Bacillales are analyzed in this work: (i) Listeriaceae (represented by Listeria monocytogenes), (ii) Paenibacillaceae (represented by Paenibacillus), and (iii) Bacillaceae (represented by the Bacillus cereus group). These families impact both food safety and food quality. Most Listeriaceae are non- pathogenic, but L. monocytogenes has one of the highest mortality rates of foodborne pathogens. Listeria spp. are often reported in food processing environments. Here, 4,430 environmental samples were collected from 9 small cheese-processing facilities and tested for Listeria and L. monocytogenes. Prevalence varied by processing facility, but across all facilities, 6.03 and 1.35% of samples were positive for L. monocytogenes and other Listeria spp., respectively. Each of these families contains strains capable of growth at refrigeration temperatures. To more broadly understand milk spoilage bacteria, genetic analyses were performed on 28 Paenibacillus and 23 B. cereus group isolates. While no specific genes were significantly associated with cold-growing Paenibacillus, the growth variation and vast genetic data introduced in this study provide a strong foundation for the development of detection strategies. Some species within the B. -
Listeria Sensu Stricto Specific Genes Involved in Colonization of the Gastrointestinal Tract by Listeria Monocytogenes
TECHNISCHE UNIVERSITÄT MÜNCHEN Lehrstuhl für Mikrobielle Ökologie Characterization of Listeria sensu stricto specific genes involved in colonization of the gastrointestinal tract by Listeria monocytogenes Jakob Johannes Schardt Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Prof. Dr. rer.nat. Siegfried Scherer Prüfende der Dissertation: 1. apl.Prof. Dr.rer.nat. Thilo Fuchs 2. Prof. Dr.med Dietmar Zehn Die Dissertation wurde am 18.01.2018 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 14.05.2018 angenommen. Table of contents Table of contents ___________________________________________________________ I List of figures _______________________________________________________________ V List of tables ______________________________________________________________ VI List of abbreviations ________________________________________________________ VII Abstract __________________________________________________________________ IX Zusammenfassung __________________________________________________________ X 1 Introduction ______________________________________________________________ 1 1.1 The genus Listeria ____________________________________________________________ 1 1.1.1 Listeria sensu stricto ________________________________________________________________