Conference Program and Abstracts

Total Page:16

File Type:pdf, Size:1020Kb

Conference Program and Abstracts Downloaded from ABSTRACTS SUBMITTED FOR THE 8TH ANNUAL CONFERENCE OF http://glycob.oxfordjournals.org/ THE SOCIETY FOR GLYCOBIOLOGY by guest on December 8, 2011 December 3–6, 2003 San Diego, California Downloaded from http://glycob.oxfordjournals.org/ by guest on December 8, 2011 ANNUAL CONFERENCE OF THE SOCIETY FOR GLYCOBIOLOGY DECEMBER 3–6, 2003 CATAMARAN RESORT HOTEL, SAN DIEGO, CA GENERAL CONFERENCE INFORMATION IMPORTANT CONFERENCE LOCATION TRANSPORTATION DEADLINES All technical sessions, posters, and exhibits will be at The Catamaran is located eleven miles from San Diego the Catamaran Resort Hotel, 3999 Mission Boulevard, International Airport. Transportation is available via San Diego, CA 92109. The Catamaran Resort Hotel offers Cloud 9 Shuttle ($8.50 one way) and taxi (approxi- August 1 Downloaded from casual elegance in a tropical paradise with acres of lush mately $18-$24 one way). Submission of nominations foliage and sun-drenched beaches. Mission Bay, the for the Karl Meyer Award world’s largest aquatic park, is at your doorstep and the PARKING enticing waves of the Pacific Ocean are just 100 yards Valet parking is available for $10 per day. Self-parking August 29 away. The Catamaran Tower commands a view from the is available for $8 per day for hotel guests. Submission of abstracts shores of La Jolla to Old Mexico and over Mission Bay to http://glycob.oxfordjournals.org/ Point Loma and the spectacular San Diego skyline. Sea PROGRAM CHAIR September 12 World, the world-famous San Diego Zoo and San Diego Jeffrey D. Esko, University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0687 Submission of applications International Airport are just minutes away. Tel: (858) 822-1100, [email protected] for the student travel stipends ACCOMMODATIONS Hotel rooms for conference participants have been set- CONFERENCE CONTACT INFORMATION October 31 aside at the Catamaran Resort Hotel. To make a reserva- tion, call the hotel directly, 858-488-1081 or (800) Conference on Glycobiology Reservations at the 422-8386. Be sure to mention that you are attending 2019 Galisteo Street, Building I-1, Santa Fe, NM 87505 Catamaran Resort Hotel the Conference on Glycobiology in order to obtain the Tel: (505) 989-4735, Fax: (505) 989-1073 by guest on December 8, 2011 conference rate. A major credit card is required to guar- [email protected] October 31 antee a reservation. The deadline for reservations at Advance conference the conference rate is October 31. Please make your HOTEL registration reservations early, as the room block will sell out. The The Catamaran Resort Hotel conference rates are: $149 for single or double. All 3999 Mission Boulevard, San Diego, CA 92109 rooms are subject to state and local taxes (currently 10.5%). Tel: (858) 488-1081, (800) 422-8386 www.catamaranresort.com PROGRAM UPDATES AND OTHER USEFUL INFORMATION: www.glycobiology.org Conference Program Overview Annual Conference of the Society for Glycobiology PROGRAM OVERVIEW 9:00 am – 5:00 pm SATELLITE SYMPOSIUM I Human Disorders of Glycosylation. Organized by Hudson H. Freeze, The Burnham Institute and Jeffrey Esko, University of California, San Diego 9:00 am – 5:00 pm SATELLITE SYMPOSIUM II Production and Quality Control for Recombinant Glycoproteins in Biotechnological Uses. Organized by Richard Cummings, University of Oklahoma and James C. Paulson, Scripps Research Institute CONFERENCE OPENING 7:00 – 7:15 pm Opening Remarks; Jeffrey Esko, President, Society for Glycobiology 7:15 – 8:45 pm SESSION I: CARBOHYDRATE DIVERSITY Wednesday, Dec 3 Chair: Gerald W. Hart, Johns Hopkins School of Medicine 8:45 – 10:00 pm RECEPTION 8:30 – 10:00 am SESSION II: GLYCOBIOLOGY AND INFECTION Chair, Marilynn Etzler, University of California, Davis 10:00 – 10:30 am Break and Exhibits 10:30 am – 12:30 pm SESSION III: GLYCOBIOLOGY AND CELL BIOLOGY Chair, Ajit Varki, University of California, San Diego Downloaded from 12:30 – 2:00 pm Lunch Break 2:00 – 4:00 pm POSTERS and EXHIBITS. Poster Topics: Carbohydrate Diversity, Glycobiology and Cell Biology, Glycobiology and Infection Thursday, Dec 4 3:00 – 4:00 pm WORKSHOP: Informatics. Anne Dell, Imperial College http://glycob.oxfordjournals.org/ 4:00 – 5:30 pm SESSION IV: GLYCANS IN PHYSIOLOGIC PROCESSES Chair, Jamey Marth, HHMI, University of California, San Diego 8:30 – 10:00 am SESSION V: GLYCOBIOLOGY AND BIOCHEMISTRY Chair: Chi-Huey Wong, The Scripps Research Institute 10:00 – 10:30 am Break and Exhibits 10:30 am – 12:30 pm SESSION VI: GLYCOBIOLOGY AND IMMUNITY Chair, Minoru Fukuda, The Burnham Institute 12:30 – 2:00 pm Lunch Break by guest on December 8, 2011 2:00 – 4:00 pm POSTERS and EXHIBITS Poster Topics: Carbohydrate Diversity, Glycobiology and Cell Biology, Glycobiology and Infection, Glycans In Physiologic Processes, Glycobiology and Biochemistry, Glycobiology and Immunity Friday, Dec 5 3:00 – 4:00 pm DISCUSSION: Reinvention of Study Sections and Your Grants Jeffrey Esko and Gerald W. Hart 4:00 – 4:45 pm BUSINESS MEETING 4:45 – 5:30 pm KARL MEYER LECTURE 7:00 – 9:30 pm BANQUET. Included with registration. Extra tickets for guests may be purchased. 8:30 – 10:00 am SESSION VII: CHEMICAL GLYCOBIOLOGY Chair, Richard Cummings, University of Oklahoma Health Sciences Center 10:00 – 10:30 am Break and Exhibits 10:30 am – 12:30 pm SESSION VIII: GLYCOBIOLOGY AND DEVELOPMENT Chair, Yu Yamaguchi, The Burnham Institute 12:30 – 2:00 pm Lunch Break 2:00 – 4:00 pm POSTERS and EXHIBITS Poster Topics: Carbohydrate Diversity, Glycobiology and Cell Biology, Glycobiology and Infection, Glycans In Physiologic Processes, Glycobiology and Biochemistry, Glycobiology and Immunity, Chemical Glycobiology, Glycobiology Saturday, Dec 6 and Development, Glycobiology and Plasticity of Nervous and Musculoskeletal Systems 4:00 – 5:30 pm SESSION IX: GLYCOBIOLOGY AND PLASTICITY OF NERVOUS AND MUSCULOSKELETAL SYSTEMS Chair, Hudson Freeze, The Burnham Institute 808 Annual Conference of the Society for Glycobiology Program Registration CONFERENCE ON GLYCOBIOLOGY ADVANCE REGISTRATION FORM DEADLINE: October 31 ON SITE REGISTRATION. After October 31, please register on site. On site registration fees will be $50 more than advance registration. On site registration begins at 3 pm, Saturday, December 3 at the Catamaran Resort Hotel. CANCELLATIONS. Notice of cancellation must be postmarked on or before November 28, 2003. A $40 processing fee will be deducted from refunds. Name Company/Institution Address City, State, Zip/Postal Code/Country Phone Fax Email SATELLITE SYMPOSIA (9 am - 5 pm, Saturday, Wednesday, December 3, $50) Check one Human Disorders of Glycosylation Production and Quality Control for Recombinant Glycoproteins in Biotechnological Uses Enter $50 for optional Satellite Symposium $ __________ Downloaded from CONFERENCE REGISTRATION Enter registration amount checked below $ __________ $380, Member of the Society for Glycobiology $480, Non-Member $175, Student (Must be full-time in a Ph.D. program or within first two years of postdoctoral training) http://glycob.oxfordjournals.org/ EXTRA BANQUET TICKETS for GUESTS, 7 pm, Wednesday, December 3. Banquet is included with registration for conference. $50 x ____ number of extra tickets Enter extra banquet amount $ __________ ENTER TOTAL AMOUNT $ __________ Check is enclosed for total amount. Please make check payable to Society for Glycobiology Charge total amount to my VISA or MasterCard. Sorry, no other cards are accepted. by guest on December 8, 2011 VISA number MasterCard number Exp date Card holder’s name Card holder’s signature Send to: Please note new address Conference on Glycobiology, 2019 Galisteo Street, Building I-1, Santa Fe, NM 87505 Fax: (505) 989-1073 (Faxed registrations must include credit card information.) SPONSORS AND EXHIBITORS The Society for Glycobiology gratefully acknowledges the support of the following companies: American Radiolabeled Chemicals Associates of Cape Cod Biogen New England Biolabs 809 Conference Program Annual Conference of the Society for Glycobiology Wednesday, December 3, 2003 Euclid Ave., St. Louis, MO 63110, [2] Mass Spec 7:15 – 8:45 PM Resource, Boston University School of Medicine, CARBOHYDRATE DIVERSITY 715 Albany St., R-806, Boston, MA 02118 ..................... 7 Gerald W. Hart, Chair 8:55 AM Regulation of Tissue Injury and Inflammation Time Abstract Number by Syndecan-1 Ectodomain Shedding; Pyong 7:15 PM Microarray Analysis of Glycosyltransferase Woo Park1, William C. Parks2, David B. Corry1, Gene Expression; James C. Paulson1, Margarida Farrah Kheradmand1 and Sheila Duncan1; [1] Amado1, Elena Comelli1, Tim Gilmartin2, Steven Department of Medicine, Baylor College of Head2, Thomas Whisenant2, Qi Yan1, Mark Sutton3 Medicine, Houston, TX, [2] Department of and Anne Dell3; [1] Department of Molecular Pediatrics, Washington University School of Biology and Experimental Medicine, The Scripps Medicine, St. Louis, MO................................................. 8 Research Institute, La Jolla, CA, [2] DNA Core 9:20 AM Reconstitution of GDP-Man Transport Activity Facility, The Scripps Research Institute, La Jolla, with Purified Leishmania LPG2 Protein in CA, [3] Department of Biological Sciences, Proteoliposomes; Hiroaki Segawa1, Stephen M. Imperial College, London, UK .......................................1 Beverley2 and Salvatore J. Turco1; [1] Dept. 7:40 PM Mass Spectrometry-Based Methods for Biochemistry, University of Kentucky Med. Ctr., Revealing the Glycans’ Family Secrets; Catherine Lexington, KY 40536, [2] Dept. Mol.
Recommended publications
  • Cryptococcus Neoformans Can Form Titan-Like Cells in Vitro in Response to Multiple Signals
    RESEARCH ARTICLE Cryptococcus neoformans can form titan-like cells in vitro in response to multiple signals Nuria Trevijano-Contador1¤, Haroldo Cesar de Oliveira1,2, RocõÂo GarcõÂa-Rodas1, SueÂlen Andreia Rossi1, Irene Llorente1, A ngel Zaballos3, Guilhem Janbon4, JoaquõÂn Ariño5, O scar Zaragoza1* 1 Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain, 2 Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, CaÃmpus Araraquara, Departamento de AnaÂlises ClõÂnicas, LaboratoÂrio de Micologia ClõÂnica, Araraquara, São Paulo, Brazil, 3 Genomics Unit, Core Scientific Services, Instituto de Salud Carlos III, a1111111111 Majadahonda, Madrid, Spain, 4 Institut Pasteur, Unite Biologie des ARN des Pathogènes Fongiques, a1111111111 DeÂpartement de Mycologie, Paris, France, 5 Institut de Biotecnologia i Biomedicina and Departament de a1111111111 BioquõÂmica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain a1111111111 a1111111111 ¤ Current address: Albert Einstein College of Medicine, Bronx, New York, United States of America. * [email protected] Abstract OPEN ACCESS Cryptococcus neoformans is an encapsulated pathogenic yeast that can change the size of Citation: Trevijano-Contador N, de Oliveira HC, GarcõÂa-Rodas R, Rossi SA, Llorente I, Zaballos AÂ, the cells during infection. In particular, this process can occur by enlarging the size of the et al. (2018) Cryptococcus neoformans can form capsule without modifying the size of the cell body, or by increasing the diameter of the cell titan-like cells in vitro in response to multiple body, which is normally accompanied by an increase of the capsule too. This last process signals. PLoS Pathog 14(5): e1007007. https://doi. leads to the formation of cells of an abnormal enlarged size denominated titan cells.
    [Show full text]
  • Molecular Genetic Characterization of Ptr Toxc-Tsc1 Interaction
    MOLECULAR GENETIC CHARACTERIZATION OF PTR TOXC-TSC1 INTERACTION AND COMPARATIVE GENOMICS OF PYRENOPHORA TRITICI-REPENTIS A Dissertation Submitted to the Graduate Faculty of the North Dakota State University of Agriculture and Applied Science By Gayan Kanishka Kariyawasam In Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY Major Department: Plant Pathology November 2018 Fargo, North Dakota North Dakota State University Graduate School Title MOLECULAR GENETIC CHARACTERIZATION OF PTR TOXC-TSC1 INTERACTION AND COMPARATIVE GENOMICS OF PYRENOPHORA TRITICI-REPENTIS By Gayan Kanishka Kariyawasam The Supervisory Committee certifies that this disquisition complies with North Dakota State University’s regulations and meets the accepted standards for the degree of DOCTOR OF PHILOSOPHY SUPERVISORY COMMITTEE: Dr. Zhaohui Liu Chair Dr. Shaobin Zhong Dr. Justin D. Faris Dr. Phillip E. McClean Dr. Timothy L. Friesen Approved: November 7, 2018 Jack Rasmussen Date Department Chair ABSTRACT Tan spot of wheat, caused by Pyrenophora tritici-repentis, is an economically important disease worldwide. The disease system is known to involve three pairs of interactions between fungal-produced necrotrophic effectors (NEs) and the wheat sensitivity genes, namely Ptr ToxA- Tsn1, Ptr ToxB-Tsc2 and Ptr ToxC-Tsc1, all of which result in susceptibility. Many lines of evidence also suggested the involvement of additional fungal virulence and host resistance factors. Due to the non-proteinaceous nature, Ptr ToxC, has not been purified and the fungal gene (s) controlling Ptr ToxC production is unknown. The objective for the first part of research is to map the fungal gene (s) controlling Ptr ToxC production. Therefore, A bi-parental fungal population segregating for Ptr ToxC production was first developed from genetically modified heterothallic strains of AR CrossB10 (Ptr ToxC producer) and 86-124 (Ptr ToxC non-producer), and then was genotyped and phenotyped.
    [Show full text]
  • Glucosyltransferase B3GLCT Gene Mutated in Peters Plus Syndrome
    RESEARCH ARTICLE Functional characterization of zebrafish orthologs of the human Beta 3- Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome Eric Weh1,2¤a, Hideyuki Takeuchi3¤b, Sanaa Muheisen1, Robert S. Haltiwanger3, Elena V. Semina1,2,4* 1 Department of Pediatrics and Children's Research Institute at the Medical College of Wisconsin, a1111111111 Milwaukee, Wisconsin, United States of America, 2 Department of Cell Biology, Neurobiology, and Anatomy at the Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 3 Complex a1111111111 Carbohydrate Research Center, University of Georgia, Athens, Georgia, United States of America, a1111111111 4 Department of Ophthalmology at the Medical College of Wisconsin, Milwaukee, Wisconsin, United States a1111111111 of America a1111111111 ¤a Current address: Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI, United States of America ¤b Current address: Department of Molecular Biochemistry, Nagoya University School of Medicine, Nagoya, Japan. * [email protected] OPEN ACCESS Citation: Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, Semina EV (2017) Functional Abstract characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene Peters Plus Syndrome (PPS) is a rare autosomal recessive disease characterized by ocular mutated in Peters Plus Syndrome. PLoS ONE 12 defects, short stature, brachydactyly, characteristic facial features, developmental delay (9): e0184903. https://doi.org/10.1371/journal. pone.0184903 and other highly variable systemic defects. Classic PPS is caused by loss-of-function muta- tions in the B3GLCT gene encoding for a 3-glucosyltransferase that catalyzes the attach- Editor: Christoph Englert, Leibniz Institute on aging β - Fritz Lipmann Institute (FLI), GERMANY ment of glucose via a β1±3 glycosidic linkage to O-linked fucose on thrombospondin type 1 repeats (TSRs).
    [Show full text]
  • Exploiting the Genetic Diversity of Wild Ancestors and Relatives of Wheat for Its Improvement Jagdeep Singh Sidhu South Dakota State University
    South Dakota State University Open PRAIRIE: Open Public Research Access Institutional Repository and Information Exchange Electronic Theses and Dissertations 2018 Exploiting the Genetic Diversity of Wild Ancestors and Relatives of Wheat for its Improvement Jagdeep Singh Sidhu South Dakota State University Follow this and additional works at: https://openprairie.sdstate.edu/etd Part of the Plant Breeding and Genetics Commons Recommended Citation Sidhu, Jagdeep Singh, "Exploiting the Genetic Diversity of Wild Ancestors and Relatives of Wheat for its Improvement" (2018). Electronic Theses and Dissertations. 2641. https://openprairie.sdstate.edu/etd/2641 This Thesis - Open Access is brought to you for free and open access by Open PRAIRIE: Open Public Research Access Institutional Repository and Information Exchange. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of Open PRAIRIE: Open Public Research Access Institutional Repository and Information Exchange. For more information, please contact [email protected]. EXPLOITING THE GENETIC DIVERSITY OF WILD ANCESTORS AND RELATIVES OF WHEAT FOR ITS IMPROVEMENT BY JAGDEEP SINGH SIDHU A thesis submitted in partial fulfillment of the requirements for the Master of Science Major in Plant Science South Dakota State University 2018 iii This thesis is dedicated to my respected father Mr. Amrik Singh Sidhu, mother Mrs. Harjit Kaur, my dear sister Sukhdeep Kaur and cute niece Samreet. iv ACKNOWLEDGEMENTS First of all, I am grateful to Dr. Sunish Sehgal for giving me an opportunity work in his winter breeding program. My master’s work would not have been possible without his love, help, support and encouragement. I truly respect Dr.
    [Show full text]
  • 2019 Abstracts
    We are delighted EDITORIAL to welcome the worldwide yeast community in Gothenburg for ICYGMB2019! The “International Yeast Conferences” started in the 1960s with a handful of delegates and since then have become THE most important event in yeast research. Now the yeast meeting to returns to Gothenburg. Many yeast researchers still remember the meeting in 2003 with over 1,100 delegates, a truly memorable event. The Life Sciences are changing, and yeast research remains at their forefront. Advancements in genome sequencing and genome editing just make yeast more exciting as model organism in basic cell biological research, genome evolution and as a tool for synthetic biology and biotechnology. One of the most important reasons for the enormous success of yeast research lies in the unique character of the international yeast research community. No other community employs such a free exchange and access to information and research tools. Nor has any other community had the ability to build – even intercontinental – consortia of critical mass to tackle large‐scale projects, such as in sequencing the first eukaryotic genome or the first comprehensive yeast knockout library. Yeast2019 is the meeting of the international yeast research community where the latest, and even unpublished results are exchanged, and new projects, alliances, and collaborations are founded. A do‐not‐miss‐event. We attempt to incorporate the present excitement in yeast research in the programme of yeast2019. We are confident that this conference will contain important news and information for all yeast researchers. Taken together, yeast2019 will provide an up‐ to‐date overview in yeast research and it will set the scene for years to come.
    [Show full text]
  • Crystal Structure of Steroid Reductase SRD5A Reveals Conserved Steroid Reduction Mechanism
    ARTICLE https://doi.org/10.1038/s41467-020-20675-2 OPEN Crystal structure of steroid reductase SRD5A reveals conserved steroid reduction mechanism Yufei Han 1,9, Qian Zhuang2,9, Bo Sun3,9, Wenping Lv 4,9, Sheng Wang5,9, Qingjie Xiao6, Bin Pang1, ✉ Youli Zhou1, Fuxing Wang1, Pengliang Chi6, Qisheng Wang3, Zhen Li7, Lizhe Zhu 4, Fuping Li8, Dong Deng6 , ✉ ✉ ✉ Ying-Chih Chiang 1 , Zhenfei Li 2 & Ruobing Ren 1 Steroid hormones are essential in stress response, immune system regulation, and repro- 1234567890():,; duction in mammals. Steroids with 3-oxo-Δ4 structure, such as testosterone or progesterone, are catalyzed by steroid 5α-reductases (SRD5As) to generate their corresponding 3-oxo-5α steroids, which are essential for multiple physiological and pathological processes. SRD5A2 is already a target of clinically relevant drugs. However, the detailed mechanism of SRD5A- mediated reduction remains elusive. Here we report the crystal structure of PbSRD5A from Proteobacteria bacterium, a homolog of both SRD5A1 and SRD5A2, in complex with the cofactor NADPH at 2.0 Å resolution. PbSRD5A exists as a monomer comprised of seven transmembrane segments (TMs). The TM1-4 enclose a hydrophobic substrate binding cavity, whereas TM5-7 coordinate cofactor NADPH through extensive hydrogen bonds network. Homology-based structural models of HsSRD5A1 and -2, together with biochemical char- acterization, define the substrate binding pocket of SRD5As, explain the properties of disease-related mutants and provide an important framework for further understanding of the mechanism of NADPH mediated steroids 3-oxo-Δ4 reduction. Based on these analyses, the design of therapeutic molecules targeting SRD5As with improved specificity and therapeutic efficacy would be possible.
    [Show full text]
  • Metabolomics Profiling Reveals New Aspects of Dolichol Biosynthesis in Plasmodium Falciparum
    bioRxiv preprint doi: https://doi.org/10.1101/698993; this version posted March 18, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Metabolomics profiling reveals new aspects of dolichol biosynthesis in Plasmodium falciparum Flavia M. Zimbres1,2#, Ana Lisa Valenciano1,2#, Emilio F. Merino1,2, Anat Florentin3,2, Nicole R. Holderman1, Guijuan He4, Katarzyna Gawarecka5, Karolina Skorupinska-Tudek5, Maria L. Fernández-Murga6, Ewa Swiezewska5, Xiaofeng Wang4, Vasant Muralidharan3,2, Maria Belen Cassera1,2* From the 1Department of Biochemistry & Molecular Biology, University of Georgia, Athens GA 30602; 2Center for Tropical and Emerging Global Diseases (CTEGD), University of Georgia, Athens GA 30602; 3Department of Cellular Biology, University of Georgia, Athens GA 30602; 4School of Plant and Environmental Sciences, Virginia Tech, Blacksburg VA 24061; 5Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland; 6Laboratory of Experimental Pathology, Health Research Institute Hospital La Fe, Valencia 46026, Spain # Contributed equally to this work * To whom correspondence should be addressed: Maria Belen Cassera: Department of Biochemistry & Molecular Biology and Center for Tropical and Emerging Global Diseases (CTEGD), University of Georgia, Athens GA 30602; [email protected]; Tel. (706) 542-5192. Keywords: Plasmodium, malaria, polyprenol, dolichol, polyprenol reductase, SRD5A3, LC-HRMS The cis-polyisoprenoid lipids namely polyprenols, dolichols and their derivatives are linear polymers of several isoprene units. In eukaryotes, polyprenols and dolichols are synthesized as a mixture of four or more homologues of different length with one or two predominant species with sizes varying among organisms.
    [Show full text]
  • Genome-Wide Association Mapping for Resistance to Leaf Rust, Stripe Rust
    Theoretical and Applied Genetics https://doi.org/10.1007/s00122-018-3086-6 ORIGINAL ARTICLE Genome‑wide association mapping for resistance to leaf rust, stripe rust and tan spot in wheat reveals potential candidate genes Philomin Juliana1 · Ravi P. Singh2 · Pawan K. Singh2 · Jesse A. Poland3 · Gary C. Bergstrom4 · Julio Huerta‑Espino5 · Sridhar Bhavani6 · Jose Crossa2 · Mark E. Sorrells1 Received: 24 June 2017 / Accepted: 12 March 2018 © The Author(s) 2018 Abstract Key message Genome-wide association mapping in conjunction with population sequencing map and Ensembl plants was used to identify markers/candidate genes linked to leaf rust, stripe rust and tan spot resistance in wheat. Abstract Leaf rust (LR), stripe rust (YR) and tan spot (TS) are some of the important foliar diseases in wheat (Triticum aestivum L.). To identify candidate resistance genes for these diseases in CIMMYT’s (International Maize and Wheat Improvement Center) International bread wheat screening nurseries, we used genome-wide association studies (GWAS) in conjunction with information from the population sequencing map and Ensembl plants. Wheat entries were genotyped using genotyping-by-sequencing and phenotyped in replicated trials. Using a mixed linear model, we observed that seedling resistance to LR was associated with 12 markers on chromosomes 1DS, 2AS, 2BL, 3B, 4AL, 6AS and 6AL, and seedling resistance to TS was associated with 14 markers on chromosomes 1AS, 2AL, 2BL, 3AS, 3AL, 3B, 6AS and 6AL. Seed- ling and adult plant resistance (APR) to YR were associated with several markers at the distal end of chromosome 2AS. In addition, YR APR was also associated with markers on chromosomes 2DL, 3B and 7DS.
    [Show full text]
  • ABSTRACT KALMAR, JACLYN. Development of Innovative
    ABSTRACT KALMAR, JACLYN. Development of Innovative Strategies for the Analyses of Complex Biological Systems Using Mass Spectrometry. (Under the direction of Dr. David C. Muddiman). Mass spectrometry (MS) is a powerful analytical tool due to its versatility, specificity, and sensitivity. MS has allowed for the proliferation in collection of molecular level –omics data, which, in turn, has provided deep insights into various complex biological systems. This work discusses new strategies for the analysis of biological molecules thought be involved in the pathogenicity of Rice blast disease and Alzheimer’s disease. Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) mass spectrometry imaging was used to identify meta-metabolomic features of Magnaporthe oryzae infected barley leaves. Three separate sets of barley were inoculated with Wild type (WT) M. oryzae, an F-box E3 ligase protein knock out (E3 ligase KO) M. oryzae, or a control solution. Over the course of the infection, each treatment was imaged using an advanced polarity switching method, allowing the detection of low and high molecular weight compounds that ionize in positive or negative polarities. Serotonin, a barley defense metabolite, was putatively identified using MS1 data then confirmed with tandem mass spectrometry fragmentation patterns. Metabolites in the melanin pathway, important for infection development of M. oryzae, were also identified using MS1 data but were unable to be confirmed due to their low abundances. Molecules related to the pathogenicity of the fungus were only found in the samples treated with the wild type M. oryzae where those treated with the genetically modified version displayed no metabolic changes related to a fungal infection.
    [Show full text]
  • Genomic and Transcriptomic Investigations Into the Feed Efficiency Phenotype of Beef Cattle
    Provided by the author(s) and NUI Galway in accordance with publisher policies. Please cite the published version when available. Title Genomic and transcriptomic investigations into the feed efficiency phenotype of beef cattle Author(s) Higgins, Marc Publication Date 2019-03-06 Publisher NUI Galway Item record http://hdl.handle.net/10379/15008 Downloaded 2021-09-25T18:07:39Z Some rights reserved. For more information, please see the item record link above. Genomic and Transcriptomic Investigations into the Feed Efficiency Phenotype of Beef Cattle Marc Higgins, B.Sc., M.Sc. A thesis submitted for the Degree of Doctor of Philosophy to the Discipline of Biochemistry, School of Natural Sciences, National University of Ireland, Galway. Supervisor: Dr. Derek Morris Discipline of Biochemistry, School of Natural Sciences, National University of Ireland, Galway. Supervisor: Dr. Sinéad Waters Teagasc, Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Grange. Submitted November 2018 Table of Contents Declaration ................................................................................................................ vii Funding .................................................................................................................... viii Acknowledgements .................................................................................................... ix Abstract ......................................................................................................................
    [Show full text]
  • TIBS-Revised Eichler and Imperiali-2017-Withfigs
    Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Citation Eichler, Jerry, and Barbara Imperiali. “Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases.” Trends in Biochemical Sciences 43, no. 1 (January 2018): 10–17. As Published https://doi.org/10.1016/j.tibs.2017.10.008 Publisher Elsevier Version Author's final manuscript Citable link http://hdl.handle.net/1721.1/119846 Terms of Use Creative Commons Attribution-NonCommercial-NoDerivs License Detailed Terms http://creativecommons.org/licenses/by-nc-nd/4.0/ Stereochemical divergence of polyprenol phosphate glycosyltransferases Jerry Eichler1 and Barbara Imperiali2 1Dept. of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel 2Dept. of Biology and Dept. of Chemistry, Massachusetts Institute of Technology, Cambridge MA, USA *correspondence to: [email protected] (Jerry Eichler) or [email protected] (Barbara Imperiali) Keywords: Dolichol phosphate, dolichol phosphate glucose synthase, dolichol phosphate mannose synthase, polyprenol phosphate, protein glycosylation, stereochemistry 1 Abstract In the three domains of life, lipid-linked glycans contribute to various cellular processes, ranging from protein glycosylation to glycosylphosphatidylinositol anchor biosynthesis to peptidoglycan assembly. In generating many of these glycoconjugates, phosphorylated polyprenol-based lipids are charged with single sugars by polyprenol
    [Show full text]
  • Prenol Nomenclature Recommendations 1986
    Eur. J. Biochem. 167,181 -184 (1987) 0 FEBS 1987 EJB - 870270 IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN) Prenol nomenclature Recommendations 1986 CONTENTS The carbon atoms along the main chain are numbered Introduction ................................. 181 from C-1, the atom that carries the hydroxyl group (C-15.11 General terms ................................ 181 of ref. 8). The methyl group carried by atom C-3 contains Pr-1. Prenol. .............................. 181 atom C-3l, that carried by atom C-7 contains atom C-7l, etc. Pr-2. Polyprenol ........................... 181 Pr-3. Esters and their derivatives . 181 Note Pr-4. Number of residues ....... 181 Stereochemistry ................. 181 This use of superscript numbers is based on section TP-2.1 Pr-5. Double bonds. ............ 181 of the recommendations for the nomenclature of tetrapyrroles Pr-6. Order of stereochemical prefixes. ............ 182 [9]. In the printing of these recommendations in the European Pr-7. Multiplicative prefixes ................... 182 Journal of Biochemistry the relevant paragraph was acci- Spec fie compounds ............................. 182 dentally transposed to the caption of Table 2. Pr-8. Trivial names ......................... 182 The repeating CsHs unit (inside the brackets of structure Pr-9. Isopentenyl diphosphate .................. 184 Pr-10. Relationship between polyprenols and isoprenoids 184 Z) is termed an isoprene unit or an isoprene residue. Prenols Pr-1 1. Juvenile hormones ...................... 184 and their esters are precursors of a variety of compounds, Pr-12. Dolichols ............................ 184 including terpenes and steroids, that have much of the carbon References ............... .............. 184 skeleton intact. Such compounds are known as isoprenoids. INTRODUCTION Pr-2. Polyprenol. Polyprenols represent a subgroup of pre- nols. The term polyprenol, already widely used (e.
    [Show full text]