RESEARCH ARTICLE Cryptococcus neoformans can form titan-like cells in vitro in response to multiple signals Nuria Trevijano-Contador1¤, Haroldo Cesar de Oliveira1,2, RocõÂo GarcõÂa-Rodas1, SueÂlen Andreia Rossi1, Irene Llorente1, A ngel Zaballos3, Guilhem Janbon4, JoaquõÂn Ariño5, O scar Zaragoza1* 1 Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain, 2 Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, CaÃmpus Araraquara, Departamento de AnaÂlises ClõÂnicas, LaboratoÂrio de Micologia ClõÂnica, Araraquara, São Paulo, Brazil, 3 Genomics Unit, Core Scientific Services, Instituto de Salud Carlos III, a1111111111 Majadahonda, Madrid, Spain, 4 Institut Pasteur, Unite Biologie des ARN des Pathogènes Fongiques, a1111111111 DeÂpartement de Mycologie, Paris, France, 5 Institut de Biotecnologia i Biomedicina and Departament de a1111111111 BioquõÂmica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain a1111111111 a1111111111 ¤ Current address: Albert Einstein College of Medicine, Bronx, New York, United States of America. *
[email protected] Abstract OPEN ACCESS Cryptococcus neoformans is an encapsulated pathogenic yeast that can change the size of Citation: Trevijano-Contador N, de Oliveira HC, GarcõÂa-Rodas R, Rossi SA, Llorente I, Zaballos AÂ, the cells during infection. In particular, this process can occur by enlarging the size of the et al. (2018) Cryptococcus neoformans can form capsule without modifying the size of the cell body, or by increasing the diameter of the cell titan-like cells in vitro in response to multiple body, which is normally accompanied by an increase of the capsule too. This last process signals. PLoS Pathog 14(5): e1007007. https://doi. leads to the formation of cells of an abnormal enlarged size denominated titan cells.