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Genetic and Antigenic Characterization of Borrelia Coriaceae, Putative Agent of Epizootic Bovine Abortion RANCE B

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Genetic and Antigenic Characterization of Borrelia Coriaceae, Putative Agent of Epizootic Bovine Abortion RANCE B JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1989, p. 389-393 Vol. 27, No. 3 0095-1137/89/030389-05$02.00/0 Copyright © 1989, American Society for Microbiology Genetic and Antigenic Characterization of Borrelia coriaceae, Putative Agent of Epizootic Bovine Abortion RANCE B. LEFEBVRE* AND GUEY-CHUEN PERNG Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California 95616 Received 15 August 1988/Accepted 7 November 1988 Borrelia coriaceae was characterized genetically and antigenically by utilizing the following techniques: restriction endonuclease analysis, Southern blotting and genomic hybridization, pulsed-field electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The B. coriaceae genome revealed unique and characteristic banding patterns both by agarose gel electrophoresis and by hybridization when compared with several Borrelia burgdorferi isolates. Pulsed-field gel electrophoresis demonstrated several linear plasmids ranging from 65 to 30 kilobase pairs. Cross-reaction with B. burgdorferi antigens ranging from 21 to 26 kilodaltons were demonstrated by immunoblotting with rabbit anti-B. coriaceae antiserum. However, most B. coriaceae antigens were quite distinct when compared with B. burgdorferi and Leptospira interrogans antigens. Borrelia coriaceae was isolated from the soft-bodied tick specifications. The restriction fragments were separated by Ornithodoros coriaceus in 1985 (16). It was classified as a gel electrophoresis in a 0.7% agarose gel at 60 V for 15.5 h. new species ofBorrelia in 1987 (12). It has been described as The gel was then stained with ethidium bromide, illuminated the putative agent of epizootic bovine abortion (12, 16, 20, with UV irradiation, and photographed. The DNA in the gel 21), although conclusive evidence of the role of the spiro- was then transferred to a nylon membrane by the method of chete as a pathogen is still lacking. However, congenital Southern (27). Reference strain ATCC 43381 B. coriaceae spirochetosis in calves has been reported (21), in which the was digested with the same restriction enzyme, radiolabeled spirochete was observed in the blood of fetuses with lesions with [a-32P]dCTP by nick translation (24), and used as the typical of epizootic bovine abortion. A highly motile, helical probe for the blot. The hybridization and subsequent washes spirochete with morphologic features similar to those seen in were carried out under stringent conditions (19). Briefly, the infected calves was isolated from the soft-bodied tick as filters were washed in 2x SSC (from a 20x stock of 3 M mentioned above (16). A monoclonal antibody directed sodium chloride and 0.3 M sodium citrate [pH 7.0]) and 0.5% against the periplasmic flagella of Borrelia burgdorferi re- sodium dodecyl sulfate and incubated at 68°C for 2 h. The acted with B. coriaceae (12). buffer was then changed, and filters were incubated for an Previous work by Hyde and Johnson (11) demonstrated additional 30 min. The blot was then dried and exposed to the relationship of B. coriaceae to several other Borrelia X-ray film. species at the genus level. The presence of a plasmid in B. Pulsed-field gel electrophoresis. Samples for pulsed-field was by them in the same manu- coriaceae also reported gel electrophoresis were prepared by the encapsulation script. method of Overhauser and Radic (22). Lambda phage DNA we B. coriaceae at the In this report further characterize concatemers used as molecular weight markers (Stratagene it with several B. genetic and antigenic level by comparing Cloning Systems, San Diego, Calif.) were prepared as pre- burgdorferi and Leptospira interrogans isolates. Restriction viously described (22, 31). Incert agarose (FMC BioProd- endonuclease analysis, Southern blotting (27), pulsed-field ucts, Rockland, Maine) was used to prepare the samples. electrophoresis (26), and Western blotting (immunoblotting) Approximately 3 ,ug of X DNA, B. burgdorferi, and B. were our this organism. utilized to increase understanding of coriaceae samples were added to each well of a 1% agarose MATERIALS AND METHODS gel. Electrophoresis was performed in a Pharmacia LKB Pulsaphor System (Pharmacia Uppsala, Sweden) with 10-s Organisms. The Borrelia organisms used in this study switching time for 44 h at a constant voltage (330 V). The were obtained from Russell Johnson, University of Minne- electrodes for south and east were set at 300, 1200, and 2100. sota. They were isolated from ticks, humans, and animals The electrodes for north and west were set at 600. (Table 1) and were maintained in modified Barbour-Stoen- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis ner-Kelly (BSK Il) medium (1) at 31°C. and Western blotting. Discontinuous sodium dodecyl sulfate- The L. interrogans isolates used in this study were ob- gel electrophoresis was performed by the method of Laem- tained from the National Leptospirosis Reference Center in mli (14) in 12% polyacrylamide gels. Whole-cell lysates from Ames, Iowa. They were maintained in EMJH medium (Difco approximately 20 ,ug were added to each lane. Lysis buffer Laboratories, Detroit, Mich.) at 29°C. and running dye concentrations were prepared according to Isolation and characterization of DNA. Whole-cell DNA Pharmacia specifications. High- and low-molecular-weight was isolated by the procedure of LeFebvre et al. (17). protein standards were obtained from Bethesda Research Restriction endonuclease analysis was performed as previ- Laboratories, Inc. (Bethesda, Md.). The gels were run at 60 ously described (29) and according to the manufacturers' mA constant current for 4 to 5.5 h. After electrophoresis the gels were prepared for Western blotting (30) with the Phar- * Corresponding author. macia Transphor system and according to the manufactur- 389 390 LEFEBVRE AND PERNG J. CLIN. MICROBIOL. TABLE 1. Borrelia and Leptospira species used Hind 11i Isolate and strain or serovar Source B. burgdorferi ATCC 35210 ......... Ixodes dammini - o m O a: 297 ......... Human spinal fluid Size in kbp P/Bi ......... Human spinal fluid 23.1 - P/Gu ......... Human skin isolate G25 ......... Ixodes ricinus 9.4 - Johnson tick ......... Ixodes dammini 6.6- B. burgdorferi-like strain 19865 ...............Rabbit kidney Borrelia sp. strain 11616...................... Shrew 4.4 - B. coriaceae ATCC 43381 ..................... Ornithodoros coriaceus L. interrogans serovars hardjo hardjoprajitno ................ Reference strain hajo bovis ................ Bovine 2 3- 2.0- ers' specifications. The nitrocellulose membranes were blocked and probed with antibody by standard procedures (6). Rabbit antiserum raised against B. coriaceae (titered at 1:12,000 by an enzyme-linked immunosorbent assay) was used to probe the blots at a dilution of 1:3,000. Biotinylated goat anti-rabbit antibody (Vector Laboratories, Burlingame, Calif.) and streptavidin-conjugated alkaline phosphatase (Kirkegaard and Perry, Gaithersburg, Md.) were then added to The according manufacturers' specifications. enzyme FIG. 1. HindIlI restriction endonuclease digest of X DNA, B. substrate, 5-bromo-4-chloro-3-indolyl phosphate, and Nitro burgdorferi human spinal fluid isolate (P/Bi), B. coriaceae ATCC Blue Tetrazolium (Kirkegaard and Perry) were added, and 43381, B. burgdorferi human skin isolate (P/Gu), and B. burgdorferi the reacting antigen-antibody complex was visualized. ATCC 35210. RESULTS 10, 11). It appears from this gel that B. coriaceae contains several linear plasmids. This assumption is based on the B. coriaceae was characterized genetically and antigeni- fact cally in this study. Figure 1 illustrates genomic DNA from B. that circular plasmids migrate very poorly in this system and coriaceae and several different B. burgdorferi isolates di- that they migrate independent of the pulse field or switching time (5, 7, 18). Conversely, the migration of linear gested with HindIII and fractionated in a 0.7% agarose gel. plasmids in this system is upon Banding differences are present between all of the Borrelia dependent these parameters. These bands remained in the same isolates on this gel. The B. coriaceae isolate was distinct at position relative to that of the linear lambda molecular markers under virtually all molecular weight sizes from the genomes repre- weight different conditions not it is sented on this gel when digested with HindIII. Digestion switching (data shown). Thus, assumed that the observed with other endonucleases such as EcoRI, BamHI, and HhaI multiple bands in the molecular size range of 25 to 65 kilobase are linear further substantiated the overall differences seen in Fig. 1 approximately pairs plasmids (data not shown). in B. coriaceae. Antigens B. coriaceae were Figure 2A illustrates a further characterization of B. expressed by also character- coriaceae and several other B. burgdorferi isolates digested ized by Western blot analysis. Figure 4 illustrates antigens from B. B. and L. sero- with HindIII. The primary purpose of this gel was to transfer coriaceae, burgdorferi, interrogans the DNA fragments to nylon membranes by the method of var hardjo reacting to rabbit antiserum raised against whole Southern (27) and to probe them with radiolabeled genomic cell B. coriaceae. Only a few weakly reacting bands could be seen of the B. coriaceae DNA. The B. coriaceae genomic DNA was Leptospira antigens. Both hardjo bovis and underloaded (1 F.g instead of 2.5 ,ug) to prevent overexpo- hardjoprajitno proteins reacted similarly
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