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Etude Des Activités Anti-Inflammatoire, Antioxydante Et Screening Par ECOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA SANTE - ED62-AMU ECOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA TERRE -UAC THESE DE DOCTORAT EN COTUTELLE Présentée publiquement et soutenue devant LA FACULTE DE MEDECINE DE MARSEILLE Le 16 Septembre 2019. Par Monsieur Martial Sênankpon GNANSOUNOU Pour obtenir le grade de DOCTEUR des UNIVERSITES d’ABOMEY CALAVI et d’AIX MARSEILLE Spécialité : Pathologies humaines-Maladies infectieuses Etude des activités anti-inflammatoire, antioxydante et screening par chromatographie gazeuse couplée à la spectrométrie de masse d’extraits éthanoliques de trois fabacées du Bénin : isolement de molécules bioactives. Membres du jury de thèse : Professeur Jean Louis MEGE Aix Marseille Université Président Professeur Sylvie RAPIOR Université de Montpellier Rapporteur Professeur Lamine Saïd BABA-MOUSSA Université d’Abomey Calavi Examinateur/Rapporteur Professeur Farid CHEMAT Université d’Avignon et des pays du Vaucluse Rapporteur Professeur Edwige AHOUSSI DAHOUENON Université d’Abomey Calavi Co-Directrice Professeur Philippe PICCERELLE Aix Marseille Université Directeur Laboratoires d’accueil : Laboratoire de Pharmacie Galénique, Pharmacotechnie Industrielle, Biopharmacie et Cosmétique-Nutrition Laboratoire d’étude et de recherches en chimie appliquée Dédicaces Je dédie cet humble travail : A ma mère, Elisabeth MEDEDA et mon père, Roger GNANSOUNOU. Sachant que vous avez transcendé toute considération terrestre, j’espère honorer votre mémoire par la présente. A mes enfants Vanessa et Michel-Ange ainsi que mon épouse A.G. Carmelle. Merci pour votre soutien et les sacrifices consentis. Votre Amour est ma motivation quotidienne. A mes frères et sœurs pour leurs encouragements. A feu Docteur Jean Pierre NOUDOGBESSI : tu fus un grand Ami et demeures un Modèle. 1 Remerciements Le présent travail de thèse en cotutelle a été co-diriger à Cotonou par Madame Edwige AHOUSSI DAHOUENON, Professeur Titulaire des Universités du CAMES. Je tiens à la remercier pour sa confiance et son soutien permanent. Madame, je vous témoigne toute ma gratitude pour m’avoir autorisé, dans des conditions initiales très difficiles, à commencer cette thèse. Merci pour votre confiance. Le Professeur Philippe PICCERELLE, Directeur de cette thèse à Aix Marseille Université, m’a accueilli avec enthousiasme dans son laboratoire en acceptant de diriger mes travaux malgré ma situation délicate, j’aimerais par la présente lui témoigner toute ma gratitude. Professeur, ce fut un honneur pour moi de travailler sous votre cordiale mais rigoureuse guidance. En m’orientant vers vous, le Professeur Jean Louis MEGE, Directeur de l’Ecole Doctorale des Sciences de la Vie et de la Santé, ne s’est nullement trompé. Je luis en sais énormément gré. Conscient du précieux temps consacré par les membres du jury de thèse à l’évaluation de ce travail, je tiens à leur dire toute ma gratitude. Merci à Mesdames Françoise MAROUZE et Samia DOAUDI Secrétaires du Professeur Jean Louis MEGE, qui avec une grande disponibilité, m’ont cordialement assisté et orienté dans toutes mes démarches administratives. Merci à Madame Valérie FILOSA qui a largement été au-delà de ses fonctions de Secrétaire par ses conseils avisés et son assistance indéfectible. Merci à Madame Stéphanie COUTIER qui a su gérer toutes mes déconvenues avec l’administration préfectorale des Bouches-du- Rhône. Je ne saurais oublier Monsieur Ulrick Arnaud SAGBO. Il a été un relais permanent et efficace entre mon Université d’origine et moi lors de mes absences du Bénin. Monsieur Arnaud : grand Merci. Cette thèse a été en partie subventionnée par la Fondation Rotary. Je tiens à ce titre à remercier Mesdames Brigitte OBELS, HANS MOEVI AKUE ainsi que Messieurs ALAIN MORALES, Philippe JEAN et Stanislas HACHEME pour leur rôle crucial dans l’obtention de cette subvention. Ma gratitude va également à l’endroit du gouvernement Béninois pour son soutien à travers le programme d’appui aux doctorants du Ministère de l’Enseignement Supérieur et de la Recherche. 2 Pour leur soutien durant ces quatre années de recherche, je voudrais également dire un grand Merci aux Professeurs, Docteurs, Ingénieurs et Doctorants du Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA) de l’Université d’Abomey Calavi et à ceux du Laboratoire de Galénique, de Cosmétologie et de Biopharmacie Industrielle d’Aix Marseille Université. Je n’ose les énumérer ici, de peur d’en oublier : Merci à tous. 3 Sommaire Dédicaces .......................................................................................................................................... 1 Remerciements ................................................................................................................................. 2 Sommaire ......................................................................................................................................... 4 Résumé ............................................................................................................................................. 5 Summary .......................................................................................................................................... 6 I- INTRODUCTION .............................................................................................................................. 7 II- Etat de l’art sur les molécules bioactives de D. guineense, P. biglobosa et T. indica ....................... 10 Article 1 : revue de littérature .......................................................................................................... 11 Senankpon Martial Gnansounou, Samy Iskandar, Maxime Robin, Jean-Michel Brunel, Edwige Dahouenon and Philippe Piccerelle. Dialium guineense Willd. Parkia biglobosa (Jacq.) R. Br. Ex Benth. And Tamarindus indica L.: Review of known and synergetic bioactive compounds. Journal of Medicinal Plants Studies 2018 ; 6(3) : 103-111. 11 III- Compositions et activités biologiques des fruits, feuilles et écorces de D. guineense, P. biglobosa et T. indica .................................................................................................................................................................................... 23 Article 2 ....................................................................................................................................................................... 24 Gnansounou, S. M., Noudogbessi, J. P., Yehouenou, B., Gbaguidi, A. N.M., Dovonon, L., Aina, M. P., Ahissou, H. and Sohounhloue, D. Proximate Composition and Micronutrient Potentials of Dialium guineense Wild growing in Benin. International Food Research Journal 21(4) : 1603-1607 (2014). ................................................................................ 24 Article 3 ....................................................................................................................................................................... 31 Ahodegnon k. Donatien, Gnansounou Martial, Bogninou G. S. Reine, Kanfon Estelle, Chabi Bienvenue, Agbangnan Dossa C. Pascal, Anago A. Eugénie, Ahoussi Edwige, Wotto Valentin and Sohounhloue C. K. Dominique. Biochemical Profile and Antioxidant Activity of Parkia biglobosa and Tamarindus indica Fruits Acclimated in Benin. Int. J. Adv. Res.2018 ; 6(11), 702-711. .......................................................................................................................................... 31 Article 4 ....................................................................................................................................................................... 43 Senankpon Martial Gnansounou, Samy Iskandar, Lydia Abou, Maxime Robin, Carole DI Giorgio, Edwige Dahouenon Ahoussi, Philippe Piccerelle and Dominique Koko Sonhounhloue. GC-MS Screening and Evaluation of the Anti- inflammatory and Antioxidant Activities of Ethanolic Leaves and Stem Barks Extracts from Dialium guineense Willd, Parkia biglobosa (Jacq.) R. Br. ex Benth. and Tamarindus indica L. Journal of Pharmacognosy and Phytochemistry 2019; 8(1): 295-301. .................................................................................................................................................... 43 IV- Conclusion Générale et perspectives ..................................................................................................................... 55 V-Références ............................................................................................................................................................... 59 VI – Annexes : Travaux collaboratifs sur les molécules bioactives naturelles .......................................................... 65 Article 5 ....................................................................................................................................................................... 66 Gbohaïda Virginie, Agbangnan D. C. Pascal, Nonviho Guévara, Gnansounou Martial, Bothon F. T. Diane, Bogninou G. S. Reine, Avlessi Félicien and Sohounhloué C. K. Dominique. Chemical Study and Evaluation of the Influence of two Physical Parameters on Polyphenols Extraction from Carapa procera Leaves. ................................................... 66 Article 6 ....................................................................................................................................................................... 81 Samy Iskandar, Martial Senankpon Gnansounou, Maxime Robin, Jean Lorquin, Carole Di Giorgio and Philippe Piccerelle.
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