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The Impact of Molecular Genetics In DOI: 10.35407/bag.2019.XXX.01 www.sag.org.ar/jbag Journal of the Argentine Society of Genetics V. XXX - No.1 July 2019 Included in: Cited by: Comité Editorial Editor General: Unamuno. Científicas y Técnicas Dra. Elsa L. Camadro Universidad de Salamanca. Mendoza, Argentina Facultad de Ciencias Agrarias Salamanca, España [email protected] Universidad Nacional de Mar del Plata [email protected] Consejo Nacional de Investigaciones Dr. Rodomiro Ortiz Científicas y Técnicas Dr. Santiago Lippold Department of Plant Breeding Balcarce, Argentina Centro de Educación Médica e Investigaciones Swedish University of Agricultural Science [email protected] Clínicas Uppsala, Suecia Buenos Aires, Argentina [email protected] Editores Asociados: [email protected] Dra. Mónica Poverene Citogenética Animal Dr. José Arturo Prada Oliveira Departamento de Agronomía Dra. Liliana M. Mola Facultad de Medicina. Departamento de Universidad Nacional del Sur Departamento de Ecología, Genética y Anatomía Humana y Embriología Bahía Blanca, Argentina Evolución Universidad de Cádiz. [email protected] Facultad de Ciencias Exactas y Naturales Cádiz, España Universidad Nacional de Buenos Aires [email protected] Mutagénesis Consejo Nacional de Investigaciones Dr. Alejandro D. Bolzán Científicas y Técnicas Genética Molecular (Animal) Laboratorio de Citogenética y Mutagénesis Buenos Aires, Argentina Dr. Guillermo Giovambattista Instituto Multidisciplinario de Biología Celular [email protected]; [email protected] Instituto de Genética Veterinaria Consejo Nacional de Investigaciones Facultad de Ciencias Veterinarias Científicas y Técnicas Citogenética Vegetal Universidad Nacional de La Plata La Plata, Argentina Dr. Julio R. Daviña Consejo Nacional de Investigaciones [email protected] Instituto de Biologia Subtropical Científicas y Técnicas Universidad Nacional de Misiones La Plata, Argentina Mutaciones Inducidas en Mejoramiento Posadas, Argentina [email protected] Vegetal [email protected] Ing. Agr. (M.Sc.) Alberto R.Prina Genética Molecular (Vegetal) Instituto de Genética “Ewald A. Favret” Genética de Poblaciones y Evolución Dr. Alberto Acevedo Centro de Investigación en Ciencias Centro de Investigación de Recursos Naturales Veterinarias y Agronómicas Dr. Jorge Cladera Instituto Nacional de Tecnología Agropecuaria Instituto Nacional de Tecnología Agropecuaria Instituto de Genética “Ewald A. Favret” Castelar, Argentina Castelar, Argentina Centro de Investigación en Ciencias [email protected] [email protected] Veterinarias y Agronómicas Instituto Nacional de Tecnología Agropecuaria Dr. Andrés Zambelli Consultor Estadístico: Castelar, Argentina Fac. de Ciencias Agrarias, Universidad [email protected] Dr. David Almorza Nacional Facultad de Ciencias del Trabajo, de Mar del Plata. Dra. Noemí Gardenal Departamento Balcarce, Argentina Facultad de Ciencias Exactas, Físicas y de Estadística e Investigación Operativa [email protected] Naturales Universidad de Cádiz. Universidad Nacional de Córdoba Cádiz, España Genética y Mejoramiento Animal Consejo Nacional de Investigaciones [email protected] Científicas y Técnicas Dra. Liliana A. Picardi Córdoba, Argentina Facultad de Ciencias Agrarias Ing. Agr. Francisco J. Babinec [email protected] Universidad Nacional de Rosario Estación Experimental Agropecuaria Anguil Zavalla, Argentina Instituto Nacional de Tecnología Agropecuaria Dr. Juan César Vilardi [email protected] Facultad de Agronomía Departamento de Ecología, Genética y Universidad Nacional de La Pampa Evolución Dra. María Inés Oyarzábal Santa Rosa, Argentina Facultad de Ciencias Exactas y Naturales Facultad de Ciencias Veterinarias [email protected] Universidad Nacional de Buenos Aires Universidad Nacional de Rosario Consejo Nacional de Investigaciones Rosario, Argentina Secretaria de Redacción: Científicas y Técnicas [email protected] Dra. María de las Mercedes Echeverría Buenos Aires, Argentina Facultad de Ciencias Agrarias [email protected] Genética y Mejoramiento Genético Vegetal Universidad Nacional de Mar del Plata Dra. Natalia Bonamico Balcarce, Argentina Genética Humana, Médica y Citogenética Facultad de Agronomía y Veterinaria [email protected] Dra. Silvia Adela Ávila Universidad Nacional de Río Cuarto Hospital Castro Rendón Río Cuarto, Argentina Diseño y maquetación: Universidad Nacional del Comahue [email protected] Mauro Salerno Nuequén, Argentina [email protected] [email protected] Dr. José Crossa Unidad de Biometría y Estadística Corrección de estilo: Dra. María Inés Echeverría Centro Internacional de Mejoramiento de Maíz Dr. Mariano Santini Instituto de Genética y Trigo (CIMMYT) [email protected] Facultad de Ciencias Médicas México, D.F., México Universidad Nacional de Cuyo [email protected] Mendoza, Argentina [email protected] Dr. Ricardo W. Masuelli Facultad de Ciencias Agrarias Imágen de tapa: Dra. María Purificación Galindo Villardón Universidad Nacional de Cuyo Polinizando Traful-biodiversidad Facultad Medicina, Campus Miguel de Consejo Nacional de Investigaciones Autora: Bárbara Casali Contenidos ARTICLE 1 PARTIAL SEQUENCES OF THE GENE THAT CODIFIES FOR THE SHORT COMMUNICATION TRANSCRIPTION FACTOR VPHSFB1 IN Vasconcellea pubescens. 7 - 9 FIRST REPORT SECUENCIAS PARCIALES DEL GEN QUE CODIFICA PARA EL FACTOR DE TRANSCRIPCIÓN VPHSFB1 EN Vasconcellea pubescens. PRIMER REPORTE Arizala-Quinto E. D, Viteri G., Idrovo-Espín F.M. ARTICLE 2 THE IMPACT OF MOLECULAR GENETICS IN PLANT BREEDING: OPINION REALITIES AND PERSPECTIVES 11 - 15 EL IMPACTO DE LA GENÉTICA MOLECULAR EN EL MEJORAMIENTO GENÉTICO VEGETAL: REALIDADES Y PERSPECTIVAS Zambelli, A. ARTICLE 3 TEST OF INTERACTION IN THE ANALYSIS OF MOLECULAR VARIANCE RESEARCH PRUEBA DE INTERACCIÓN EN EL ANÁLISIS MOLECULAR DE VARIANZA 17 - 23 Bruno C., Videla M.E. , Balzarini M. ARTICLE 4 GENOTYPIC DIVERSITY IN 291 MAIZE LINES FROM CIMMYT AND RESEARCH PHENOTYPIC CHARACTERIZATION IN SOUTHERN CORDOBA, 25 - 33 ARGENTINA DIVERSIDAD GENOTIPICA DE 291 LÍNEAS DE MAÍZ DE CIMMYT Y CARACTERIZACIÓN FENOTÍPICA EN EL SUR DE CÓRDOBA, ARGENTINA Rossi E.A., Ruiz M., Di Renzo M., Bonamico N.C. ARTICLE 5 TAKING ADVANTAGE OF ORGANELLE GENOMES IN PLANT BREEDING: REVIEW ARTICLE AN INTEGRATED APPROACH 35 - 51 TAKING ADVANTAGE OF ORGANELLE GENOMES IN PLANT BREEDING: AN INTEGRATED APPROACH Colombo N. Vol XXX (1): 7-9; July 2019 Journal of the Argentine Society of Genetics PARTIAL SEQUENCES OF THE GENE THAT CODIFIES FOR THE TRANSCRIPTION FACTOR VPHSFB1 IN Vasconcellea pubescens. FIRST REPORT SECUENCIAS PARCIALES DEL GEN QUE CODIFICA PARA EL FACTOR DE TRANSCRIPCIÓN VPHSFB1 EN Vasconcellea pubescens. PRIMER REPORTE Arizala-Quinto E. D 1, Viteri G. 1, Idrovo-Espín F.M. 1,2 ABSTRACT 1 Universidad de Las Américas UDLA. Plant heat stress transcription factors (HSFs) are involved in the response to heat. In Facultad de Ingeniería y Ciencias Aplicadas. Calle José Queri s/n Arabidopsis thaliana the HSFs genes are completely identified, however there was no entre Av. Eloy Alfaro y Granados. information available about these genes in Vasconcellea pubescens (Chamburo) until now. Quito-Ecuador In this preliminary work we describe the VPHSFB1 gene of V. pubescens (gene expression evaluated by RT-PCR and the partial sequence) that was induced by the increment of 2 Universidad Central del Ecuador. temperature. From our results, VPHSFB1 could be used as a heat response marker gene in Facultad de Ciencias Químicas. Francisco Viteri s/n y Gato Sobral. tropical species. Quito-Ecuador Key words: Caricaceae, gene expression, heat. Corresponding author: F.M Idrovo-Espín [email protected] RESUMEN Los factores de transcripción del estrés por calor en plantas (HSFs) están involucrados en la respuesta al calor. En Arabidopsis thaliana los genes HSFs están completamente identificados, Cite this article as: Arizala-Quinto E. D, Viteri G. , Idrovo- sin embargo no había información disponible sobre estos genes en Vasconcellea pubescens Espín F.M. 2019. PARTIAL SEQUENCES (Chamburo) hasta ahora. En este trabajo preliminar describimos el gen VPHSFB1 de V. OF THE GENE THAT CODIFIES FOR THE pubescens (expresión génica evaluada por RT-PCR y la secuencia parcial) que fue inducido TRANSCRIPTION FACTOR VPHSFB1 por el incremento de temperatura. A partir de nuestros resultados, se podría usar a VPHSFB1 IN Vasconcellea pubescens. FIRST REPORT. BAG. Journal of Basic and como un gen marcador de respuesta a calor en especies tropicales. Applied Genetics XXX (1): 7-9. Palabras clave: Caricaceae, expresión génica, calor. Received: 12/12/2017 Accepted: 10/08/2018 INTRODUCTION Author’s approval of galley proofs: 05/14/2019 Plant heat stress transcription factors (HSFs) are essential components of the General Editor: Elsa Camadro signal transduction involved in the expression of genes responsive to this kind of abiotic stress (Nover et al., 2001). In A. thaliana 21 members of HSFs belonging DOI: 10.35407/bag.2019.XXX.01.01 to three genes classes A, B and C, have been identified (Kotaket al., 2004). Among ISSN online version: 1852-6233 these, ATHSFB1 (Class B) is necessary for the expression of heat stress inducible genes (as heat shock protein genes) that are involved in thermotolerance (Ikeda et al., 2011). Caricaceae is a family composed by six genera, two of them are Vasconcellea and Carica. The 21 species that belong to genus Vasconcellea (collectively known as highland papayas) are distributed in South America,
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