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Bacteroides Gingivalis, and Bacteroides Endodontalis in a New Genus, Porphyromonas H

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Bacteroides Gingivalis, and Bacteroides Endodontalis in a New Genus, Porphyromonas H INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Jan. 1988, p. 128-131 Vol. 38, No. 1 0020-7713/88/010128-04$02.00/0 Copyright 0 1988, International Union of Microbiological Societies Proposal for Reclassification of Bacteroides asaccharolyticus , Bacteroides gingivalis, and Bacteroides endodontalis in a New Genus, Porphyromonas H. N. SHAH1* AND M. D. COLLINS’ Department of Oral Microbiology, London Hospital Medical College, London El 2AD,l and Division of Microbiology, AFRC Institute of Food Research, Shinfeld, Reading RG2 9AT,= United Kingdom The asaccharolytic, pigmented Bacteroides, Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacte- roides endodontalis, form a group of relatively homogeneous species which differ markedly in biochemical and chemical properties from the type species of Bacteroides, Bacteroides fragilis (Castellani and Chalmers), such that they should not be retained within this genus. Therefore, we propose that Bacteroides asaccharolyticus (Holdeman and Moore) Finegold and Barnes, Bacteroides gingivalis Coykendhll, Kaczmarek and Slots, and Bacteroides endodontalis van Steenbergen, van Winkelhoff, Mayrand, Grenier and de Graaff be reclassified in a new genus, Porphyromonas, as Porphyromonas asaccharolytica comb. nov., Porphyromonas gingivalis comb. nov., and Porphyromonas endodontalis comb. nov., respectively. In Bergey ’s Manual of Determinative Bacteriology, 8th quite unrelated to the type species of the genus Bacteroides, ed. (12), the asaccharolytic, pigmented Bacteroides were Bacteroides fragilis. All of the asaccharolytic, pigmented regarded as a single homogeneous taxon, Bacteroides mela- bacteroides accumulate major levels of protoheme rather ninogenicus subsp. asaccharolyticus. As the clinical signif- than protoporphyrin when cells are cultured on blood agar icance of these microorganisms in oral cavities was recog- (3,25, 27). The three species are nonfermentative and utilize nized, extensive taxonomic studies were carried out. nitrogenous substrates such as Trypticase and Proteose Heterogeneity was first demonstrated among these bacteria Peptone as energy sources (28a, 28b). The metabolic end through studies of their deoxyribonucleic acid (DNA) base products from these substrates include significant levels of compositions and enzyme polymorphism, and two regions of n-butyric acid in addition to other volatile fatty acids (11). variation were reported (29). These findings were substanti- Furthermore, particular amino acids, such as aspartate, are ated by sodium dodecyl sulfate polypeptide patterns (34) and preferentially catabolized (28a, 28b), and strains generally DNA-DNA hybridization data (36). As a result of these studies on the asaccharolytic, pigmented strains, the group possess proteolytic activities (16,19). Unlike other members containing strains with high guanine-plus-cytosine (G+C) of the genus Bacteroides, which .contain predominantly contents (52 to 54 mol%) and an electrophoretically fast- 12-methyl-tetradecanoic acid (anteiso-C,,,, acid) as their migrating malate dehydrogenase (MDH) was reclassified as a long-chain fatty acid, the asaccharolytic, pigmented species new species, Bacteroides asaccharolyticus (7, 29). Further contain mainly 13-methyl-tetradecanoicacid (iso-C,,,, acid). differences between B. asaccharolyticus and the group of The latter taxa also contain MDH and glutamate dehy- strains with low G+C contents (46 to 48 mol%) and a drogenase but differ from the type species of the genus slow-migrating MDH (29) were revealed by an analysis of Bacteroides., B. fragilis, in lacking enzymes of the hexose tlheir lipids (26). Strains of B. asaccharolyticus possess very monophosphate shunt-pentose phosphate pathway (viz., high levels of 13-methyl-tetradecanoic acid (iso-C,,,, acid) glucose-6-phosphate dehydrogenase, 6-phosphogluconate and menaquinones with 10 isoprene units (MK-lo), whereas dehydrogenase) (27, 28). All of the asaccharolytic, pig- tlhe group of strains with low G+C contents contains signifi- mented species examined so far have DNA base composi- cantly lower levels of iso-C,,:, fatty acids and menaquinones tions within the range from 46 to 54 mol% G+C. These taxa with nine isoprene units (MK-9) (26). The latter group of also differ from other Bacteroides species studied in lacking strains was subsequently reclassified as Bacteroides gingi- diaminopimelic acid in their cell walls (8, 29). valis (4). It is generally believed that most oral, asaccharoly- We have suggested previously that the genus Bacteroides tic, black-pigmented Bacteroides strains belong to B. gingi- should be restricted to the type species, B. fragilis, and valis, whereas most nonoral or clinical isolates are B. related taxa (3, 27). These related taxa include Bacteroides asaccharolyticus strains. Recently, a third asaccharolytic distasonis, Bacteroides eggerthii, Bacteroides ovatus, Bac- species, Bacteroides endodontalis, which was isolated from teroides thetaiotaomicron, Bacteroides uniformis, and Bac- infected dental root canals, has been proposed (37). Pheno- teroides vulgatus and, more recently, Bacteroides caccae, typically, B. endodontalis appears to be more closely related Bacteroides merdae, and Bacteroides stercoris (14). The to B. asaccharolyticus than to B. gingivalis and possesses a organisms in the “B. fragilis group” have relatively low DNA base composition of ca. 50 to 51 mol% G+C (37). The G+C contents, ranging from 40 to 48 mol% (13), and the MDH of B. endodontalis migrates faster than the MDHs of B. species are relatively homogeneous biochemically (27, 28). asaccharolyticus and B. gingivalis (Shah, unpublished data). B. fragilis and related species are saccharolytic and gener- B. asaccharolyticus, B. gingivalis, and B. endodontalis ally produce acetic and succinic acids as the major end are well-defined species and on the basis of biochemical and products of glucose metabolism (11). They also differ from chemical properties form a relatively homogeneous group the asaccharolytic, pigmented Bacteroides species in pos- sessing enzymes of the hexose monophosphate shunt-pen- * Corresponding author tose phosphate pathway (27, 28) and in their cellular fatty 128 VOL. 38, 1988 NOTES 129 acids (23, 26). Furthermore, the dibasic amino acid of their Major fermentation products from BM or PYG medium peptidoglycan is meso-diaminopimelic acid (29). are n-butyric and acetic acids; lower levels of propionic, Thus, there is now overwhelming biochemical and chem- isobutyric, and isovaleric acids are also produced. Phenyla- ical evidence that the asaccharolytic, pigmented Bacteroides cetic acid is not produced. Proteolytic activity is low, but species differ so markedly from the type species of the genus gelatin liquefaction is positive and fibrinolytic activity is Bacteroides, B. fragilis, as to warrant placement in a sepa- present. Indole is produced. a-Fucosidase is produced. rate genus. Therefore, we formally propose that the species Nitrate is not reduced to nitrite. Starch and esculin are not presently designated B. asaccharolyticus (7), B. gingivalis hydrolyzed. Cells do not agglutinate sheep erythrocytes. (4), and B. endodontalis (37) be reclassified in a new genus, MDH and glutamate dehydrogenase are present; glucose-6- Porphyromonas, as Porphyromonas asaccharolytica comb, phosphate dehydrogenase and 6-phosphogluconate dehydro- nov., Porphyromonas gingivalis comb. nov., and Porphyro- genase are absent. monas endodontalis comb. nov., respectively. The cell wall peptidoglycan contains lysine; 2-keto-3- Description of Porphyromonas gen. nov. (Por.phy . deoxyoctulosonic acid is absent. The principal respiratory ro.mo’nas. Gr. adj. porphyreos purple; Gr. n. monas unit; quinones are menaquinones with 10 isoprene units. Both N. L. fem.n. Porphyromonas porphyrin cell). The descrip- nonhydroxylated and 3-hydroxylated fatty acids are present. tion below is based on our own observations and previous The nonhydroxylated fatty acids are composed of predomi- descriptions of the asaccharolytic, pigmented Bacteroides nantly iso-methyl branched types, with iso-C,,:, acid pre- species (2,-11, 16, 20, 22, 23, 26-33, 35-38, 42, 43, 45). dominating. Gram-negative, obligately anaerobic, nonsporeforming, The DNA base compositions range between 52 and 54 nonmotile rods or coccobacilli. Most cells in broth are small mol% G+C. (0.5 to 0.8 by 1.0 to 3.5 pm), but occasionally longer cells (4 The type strain is strain ATCC 25260. to 6 pm) may be formed. Colonies on blood agar plates are Description of Porphyromonas gingivalis (Coykendall, Kacz- smooth (rarely rough), shiny, convex, and 1 to 3 mm in marek and Slots) comb. nov. The description below is based diameter and darken progressively from the edge toward the on previous studies (1, 3-5, 8-11, 15-19, 21, 23, 25-29, 31, center after 6 to 10 days. Eventually the whole colony 33, 35, 36, 38, 39; G. Sundqvist, Ph.D. thesis, Umea Uni- becomes black due to protoheme production. Growth is not versity of Odontology, Umea, Sweden, 1976) and our own significantly affected by carbohydrates. Nitrogenous sub- observations. strates such as Proteose Peptone, Trypticase, and yeast Gram-negative, obligately anaerobic, nonsporeforming, extract markedly enhance growth. The optimum tempera- nonmotile rods or coccobacilli. Cells in broth are 0.5 by 1 to ture for growth is 37°C. Major fermentation products from 2 pm. Cells from solid media are coccobacilli or very short BM (29) or PYG (11) medium are n-butyric and acetic acids; rods. Colonies on blood agar plates are
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