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Mise Au Point D'une Technique De Diagnostic Sérologique Par Open Archive TOULOUSE Archive Ouverte ( OATAO ) OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in : http://oatao.univ-toulouse.fr/ Eprints ID : 10732 To cite this version : Lenfant, François. Mise au point d’une technique de diagnostic sérologique par immunofluorescence indirecte de la Besnoitiose bovine à Besnoitia besnoiti. Thèse d'exercice, Médecine vétérinaire, Ecole Nationale Vétérinaire de Toulouse - ENVT, 2013, 125 p. Any correspondance concerning this service should be sent to the repository administrator: [email protected]. ANNEE 2013 THESE : 2013 – TOU 3 – 4060 MISE AU POINT D’UNE TECHNIQUE DE DIAGNOSTIC PAR IMMUNOFLUORESCENCE INDIRECTE DE LA BESNOITIOSE BOVINE A BESNOITIA BESNOITI _________________ THESE pour obtenir le grade de DOCTEUR VETERINAIRE DIPLOME D’ETAT présentée et soutenue publiquement devant l’Université Paul-Sabatier de Toulouse par LENFANT François Né, le 21 avril 1987 à POITIERS (86) ___________ Directeur de thèse : M. Emmanuel LIENARD ___________ JURY PRESIDENT : M. Alexis VALENTIN Professeur à l’Université Paul-Sabatier de TOULOUSE ASSESSEURS : M. Emmanuel LIENARD Maître de Conférences à l’Ecole Nationale Vétérinaire de TOULOUSE M. Michel FRANC Professeur à l’Ecole Nationale Vétérinaire de TOULOUSE MEMBRE INVITÉ : M. Jean-Pierre ALZIEU Docteur vétérinaire au Laboratoire Vétérinaire Départementale de l’Ariège Ministère de l'Agriculture de l’Agroalimentaire et de la Fôret ECOLE NATIONALE VETERINAIRE DE TOULOUSE Directeur : M. A. MILON PROFESSEURS CLASSE EXCEPTIONNELLE M. AUTEFAGE André, Pathologie chirurgicale M. CORPET Denis, Science de l'Aliment et Technologies dans les Industries agro-alimentaires M DELVERDIER Maxence, Anatomie Pathologique M. ENJALBERT Francis, Alimentation M. EUZEBY Jean, Pathologie générale, Microbiologie, Immunologie M. FRANC Michel, Parasitologie et Maladies parasitaires M. MARTINEAU Guy, Pathologie médicale du Bétail et des Animaux de Basse-cour M. PETIT Claude, Pharmacie et Toxicologie M. REGNIER Alain, Physiopathologie oculaire M. SAUTET Jean, Anatomie M. SCHELCHER François, Pathologie médicale du Bétail et des Animaux de Basse-cour PROFESSEURS 1° CLASSE M. BERTHELOT Xavier, Pathologie de la Reproduction M. BOUSQUET-MELOU Alain, Physiologie et Thérapeutique Mme CLAUW Martine, Pharmacie-Toxicologie M. CONCORDET Didier, Mathématiques, Statistiques, Modélisation M. FOUCRAS Gilles, Pathologie des ruminants M. LEFEBVRE Hervé, Physiologie et Thérapeutique PROFESSEURS 2° CLASSE Mme BENARD Geneviève, Hygiène et Industrie des Denrées alimentaires d'Origine animale M. BERTAGNOLI Stéphane, Pathologie infectieuse Mme CHASTANT-MAILLARD Sylvie, Pathologie de la Reproduction M. DUCOS Alain, Zootechnie M. DUCOS DE LAHITTE Jacques, Parasitologie et Maladies parasitaires Mme GAYRARD-TROY Véronique, Physiologie de la Reproduction, Endocrinologie M. GUERRE Philippe, Pharmacie et Toxicologie Mme HAGEN-PICARD Nicole, Pathologie de la Reproduction M. JACQUIET Philippe, Parasitologie et Maladies Parasitaires M. LIGNEREUX Yves, Anatomie M MEYER Gilles, Pathologie des ruminants M. PICAVET Dominique, Pathologie infectieuse M. SANS Pierre, Productions animales Mme TRUMEL Catherine, Pathologie médicale des Equidés et Carnivores PROFESSEURS CERTIFIES DE L'ENSEIGNEMENT AGRICOLE Mme MICHAUD Françoise, Professeur d'Anglais M SEVERAC Benoît, Professeur d'Anglais MAITRES DE CONFERENCES HORS CLASSE M. BAILLY Jean-Denis, Hygiène et Industrie des Denrées alimentaires d'Origine animale M. BERGONIER Dominique, Pathologie de la Reproduction Mlle BOULLIER Séverine, Immunologie générale et médicale Mme BOURGES-ABELLA Nathalie, Histologie, Anatomie pathologique M. BRUGERE Hubert, Hygiène et Industrie des Denrées alimentaires d'Origine animale Mlle DIQUELOU Armelle, Pathologie médicale des Equidés et des Carnivores M. JOUGLAR Jean-Yves, Pathologie médicale du Bétail et des Animaux de Basse-cour Mme LETRON-RAYMOND Isabelle, Anatomie pathologique M. LYAZRHI Faouzi, Statistiques biologiques et Mathématiques M. MATHON Didier, Pathologie chirurgicale Mme PRIYMENKO Nathalie, Alimentation MAITRES DE CONFERENCES (classe normale) M. ASIMUS Erik, Pathologie chirurgicale Mme BENNIS-BRET Lydie, Physique et Chimie biologiques et médicales Mlle BIBBAL Delphine, Hygiène et Industrie des Denrées alimentaires d'Origine animale Mme BOUCLAINVILLE-CAMUS Christelle, Biologie cellulaire et moléculaire Mlle CADIERGUES Marie-Christine, Dermatologie M. CONCHOU Fabrice, Imagerie médicale M. CORBIERE Fabien, Pathologie des ruminants M. CUEVAS RAMOS Gabriel, Chirurgie Equine Mme DANIELS Hélène, Microbiologie-Pathologie infectieuse M. DOSSIN Olivier, Pathologie médicale des Equidés et des Carnivores Mlle FERRAN Aude, Physiologie M. GUERIN Jean-Luc, Elevage et Santé avicoles et cunicoles M. JAEG Jean-Philippe, Pharmacie et Toxicologie Mlle LACROUX Caroline, Anatomie Pathologique des animaux de rente M. LIENARD Emmanuel, Parasitologie et maladies parasitaires M. MAILLARD Renaud, Pathologie des Ruminants Mme MEYNAUD-COLLARD Patricia, Pathologie Chirurgicale M. MOGICATO Giovanni, Anatomie, Imagerie médicale M. NOUVEL Laurent, Pathologie de la reproduction Mlle PALIERNE Sophie, Chirurgie des animaux de compagnie Mlle PAUL Mathilde, Epidémiologie, gestion de la santé des élevages avicoles et porcins Mme PRADIER Sophie, Médecine interne des équidés M. RABOISSON Didier, Productions animales (ruminants) Mme TROEGELER-MEYNADIER Annabelle, Alimentation M. VOLMER Romain, Microbiologie et Infectiologie (disponibilité à cpt du 01/09/10) M. VERWAERDE Patrick, Anesthésie, Réanimation Mme WARET-SZKUTA Agnès, Production et pathologie porcine MAITRES DE CONFERENCES et AGENTS CONTRACTUELS M. BOURRET Vincent, Microbiologie et infectiologie Mme FERNANDEZ Laura, Pathologie de la reproduction ASSISTANTS D'ENSEIGNEMENT ET DE RECHERCHE CONTRACTUELS Mlle DEVIERS Alexandra, Anatomie-Imagerie M. DOUET Jean-Yves, Ophtalmologie Mlle LAVOUE Rachel, Médecine Interne Mlle PASTOR Mélanie, Médecine Interne M VERSET Michaël, Chirurgie des animaux de compagnie Remerciements Aux membres du jury de thèse A Monsieur le Professeur Alexis VALENTIN Professeur des Universités Praticien Hospitalier Zoologie et Parasitologie Qui nous fait l’honneur d’accepter de présider notre jury de thèse, Hommages respectueux. A Monsieur le Docteur Emmanuel LIENARD Maître de Conférences à l’Ecole Nationale Vétérinaire de Toulouse Parasitologie et Maladies parasitaires Qui nous a fait l’honneur d’accepter la direction de notre thèse, Pour sa disponibilité, son écoute, ses conseils, Qu’il reçoive ma profonde reconnaissance, Et mes sincères remerciements. A Monsieur le Professeur Michel FRANC Professeur de l’Ecole Nationale Vétérinaire de Toulouse Parasitologie et Maladies parasitaires Qui nous a fait l’honneur de prendre part à notre jury et son soutien à cette étude, Sincères remerciements. A Monsieur le Docteur Jean-Pierre ALZIEU Docteur vétérinaire au Laboratoire Vétérinaire Départementale de l’Ariège Qui nous fait l’honneur d’accepter d’assister à cette thèse, Pour sa contribution à ce travail, Hommages respectueux. Aux personnes ayant aidé à l’aboutissement de cette thèse A Monsieur le Professeur Philippe JACQUIET Professeur de l’Ecole Nationale Vétérinaire de Toulouse Parasitologie et Maladies parasitaires Qui m’a confié ce sujet, Pour son écoute, ses conseils, Qu’il reçoive mes sincères remerciements. Au Service de Parasitologie Et plus particulièrement Christelle GRISET et Françoise PREVOT, Pour leur aide précieuse durant ce travail de thèse. « Je crois que vous vous trompez si vous pensez que le bonheur dans la vie réside principalement dans les relations humaines. Le bonheur se trouve tout autour de nous, il est partout, il est dans tout ce que nous pouvons expérimenter. Et pour le savoir il faut changer le regard que nous portons sur les choses. » Chris McCandless A ma famille A mes parents, pour votre soutien durant ces longues années studieuses, malgré la distance qui nous sépare. Vous m’avez donné tous les moyens pour arriver au bout, je vous dois cette réussite. Merci infiniment... A ma sœur, pour ta bonne humeur et ton optimisme inconditionnels. Tu as su réussir ta vie, je suis très fier d’avoir une sœur comme toi. A ma mamie Simonne, parce que tu as toujours cru en ma réussite, je sais que tu as beaucoup prié pour moi, merci du fond du cœur. Je suis tellement fier d’avoir une super mamie comme toi. A ma mamie Jeannette et mon papy Jacques, pour votre gentillesse, pour tous les bons moments passés en votre compagnie, chez vous, chez Jean-Pierre, aux Etats-Unis, et pour tout l’amour que vous témoignez envers vos petits-enfants. A mes amis… … de longues dates A Antoine, Nicolas et Quentin, les compères, les mousquetaires, pour notre amitié sans faille depuis plus de 20 ans, pour tous ces beaux moments qu’ils nous restent à vivre ensemble. … de lycée A Ryadh, pour ton amitié vieux frère, pour nos sketchs, ta grande gueule, nos vacances à Saint-Georges-de-Didonne, nos moments de « galériens ». A Arnaud, pour ton humour, nos fous rires en cours, nos sketchs, nos soirées un peu trop arrosées à Limoges (qui parle de Ti punch ?). A Coraline, pour ta bonne humeur caractéristique, ta fraise, ta générosité, et pour nos futures aventures australiennes à Sydney. A Maxime, Kévin, Martial, pour tous les bons moments passés ensemble au lycée et depuis, et tous ceux à venir, en commençant par l’hiver prochain ! … de prépa A Frankie, pour tous les bons
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